CN103598447A - Non-antibiotic sucking pig milk replacer and preparation method - Google Patents
Non-antibiotic sucking pig milk replacer and preparation method Download PDFInfo
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Abstract
The invention discloses a non-antibiotic sucking pig milk replacer, which comprises various raw materials as follows: puffed corn, puffed soybean, plasma protein powder, steamed fish meal, soybean protein concentrate, low protein whey powder, lactose, fermented soybean meal, glucose, cane sugar, salt, anti-oxidant, lysine, threonine, methionine, tryptophan, zinc oxide, butyrin, microecology preparation, compound acidifying agent, compound enzyme preparation, compound vitamin premix and compound mineral matter premix. The invention employs synergism between butyrin, zinc oxide, acidifying agent and microecology preparation, and in the prerequisite that the antibiotics are not used, the product can help pigling to realize early stage weaning and get rid of weaning stress, and adjust sucking pig gastrointestinal tract health, control diarrhea, increase survival rate, and comprehensively improve immunity and disease resistance of sucking pig.
Description
Technical field
The invention belongs to field of fodder, relate to a kind of piglet feed, particularly a kind of antibiotic-free piglet preweaning feed and preparation method thereof.
Background technology
Animal is used the antibiotic history of existing 1 year, as the medicine for the treatment of, diseases prevention, health care and growth promoting function, animal husbandry development has been played to huge facilitation, still extensive use in animal husbandry now.But along with scientific progress and people develop improving constantly of understanding to biology, antibiotic is also day by day clear and definite to the mankind's harm and potential harm.
Abuse of antibiotics can destroy colony balance in animal intestinal; Can make bacterial drug resistance strengthen and antibody-resistant bacterium increase; While not feeding antibiotic, animal resistance is not poor, and health normal development is influenced; Cause livestock products medicament residue, residual antibiotic can slowly be gathered in human body and other plant by livestock products and environment, finally can come together in by all means human body.European Union and other developed countries limit and use and livestock products examination criteria is improved antibiotic, directly affect the outlet of China's livestock products.The cry of forbidding abuse of antibiotics resounds through all over the world, has also caused the great attention of China, but forbidding antibiotic can bring huge economic loss.China Shi Yige developing country, the animal husbandry mode of production family's raising is main, and production environment condition is poor, and the level of production is not high, strong to antibiotic dependence, thereby present stage is sought the extremely urgent thing that other material substitute antibiotics are for we.
Along with the development of domestic pig industry, the Weaning Age of piglet is also constantly shifted to an earlier date.From the wean of average 28 days of past, be advanced to gradually 21 days, even before 21 days.Thereby the time of nursing of sow that not only can shorten early weaning piglet improves the breeding potential of sow and the production cost of a year litter size, raising childbirth house utilization rate, reduction piglet, and can block the propagation of some infectious disease.
Early-weaned piglets is because digestive system development is immature, digestive ferment system is unsound, gastric acid secretion is not enough, the antibody that during wean, parent provides stops and the main immune system of self is imperfect, on disease and stress resistivity decline add be subject to physiological environment nutrition stress wait factor to affect to cause that piglet appetite is poor, indigestion, growth inhibition, efficiency of feed utilization is low, diarrhoea is even dead.
Publication number is that the Chinese invention patent application of CN1513347 was on July 21st, 2004, this patent discloses the application of tributyrin as feed addictive, and the consumption of tributyrin, according to feed gross weight meter, for 0.1%-0.2%, 0.5%-1.0% preferably.
Although tributyrin is used and can play the function that regulates Gastrointestinal Tract of Weanling Piglets as feed addictive, improve efficiency of feed utilization, but rely on separately tributyrin can not play the effect of substitute antibiotics completely, also reckon without synergy and the impact of processing technology on its function of other raw material and additive and tributyrin simultaneously.
Summary of the invention
The object of the invention is in early days to use in weanling pig feed easy to digest, easily absorb, without the raw material of ANFs; In order to reduce antibiotic use, in feed, add some and can promote the functional feedstuff additives such as intestine of young pigs is grown, enhancing immunity, the production performance that improves, control diarrhea to carry out substitute antibiotics simultaneously.
In order to realize foregoing invention object, the invention provides nonreactive piglet preweaning feed, it comprises granulate for the first time raw material and the raw material of granulating for the second time, wherein, the described raw material of granulating for the first time comprises the component of following weight portion: popcorn 35-50 part, expanded soybean 15-22 part, steam fish meal 3-5 part, FSPC 2-5 part and fermented bean dregs 1-4 part, the described raw material of granulating for the second time comprises the component of following weight portion: SDPP 2-4 part, low albumen whey powder 10-15 part, lactose 5-10 part, glucose 3-5 part, sucrose 2-5 part, salt 0.1-0.3 part, antioxidant 0.05-0.1 part, lysine 0.1-0.3 part, threonine 0.1-0.3 part, methionine 0.1-0.3 part, tryptophan 0.01-0.1 part, zinc oxide 0.1-0.3 part, tributyrin 0.1-0.4 part, probiotics 0.05-0.2 part, compound acidulant 0.1-0.4 part, complex enzyme formulation 0.05-0.2 part, complex vitamin premix 0.1-0.3 part, composite mineral matter premix 0.1-0.3 part.
Wherein, described complex enzyme formulation comprises amylase 1300U/g, protease 2500U/g, cellulase 1500U/g, pectase 2000U/g, phytase 50U/g and 1,4 beta-glucanase 3500U/g.
Wherein, described probiotics comprises bacillus subtilis, lactobacillus acidophilus, Pediococcus acidilactici, Yue Shi lactobacillus and S. cervisiae, total effective viable bacteria content>=1 * 10
9cFU/g.
Wherein, described bacillus subtilis is bacillus subtilis CGMCC No.6511, described lactobacillus acidophilus is lactobacillus acidophilus CGMCC No.6499, described Pediococcus acidilactici is Pediococcus acidilactici CGMCC No.6500, described Yue Shi lactobacillus is Yue Shi lactobacillus CGMCC No.4926, and described S. cervisiae is S. cervisiae No.6560.
Wherein, described acidulant is mainly comprised of fumaric acid, malic acid, citric acid and phosphoric acid, adopts vegetable fat coated.
Wherein, described antioxidant is one or more of L-AA, Butylated Hydroxytoluene, fourth hydroxyl methoxy benzene, tocopherol, ethoxyquinoline etc.
Wherein, in the premix of every 1kg composite mineral matter, containing 35000mg copper, 40000mg iron, 15000mg manganese, 26000mg zinc, 100mg selenium, 120mg iodine, carrier is stone flour.
Wherein, in every 1kg complex vitamin premix, contain 4,000 ten thousand IU vitamin As, 5,000,000 IU vitamin Ds
3, 35g vitamin E, 8500mg vitamin K, 6g Cobastab
1, 18g Cobastab
2, 10g vitamin B6,50mg Cobastab
12, 1500mg folic acid and 1500mg biotin, carrier is time powder and medical stone powder.
The present invention also provides the preparation method of described nonreactive piglet preweaning feed, and it comprises the steps:
Granulate for the first time: take by weight popcorn, expanded soybean, steam fish meal, FSPC, fermented bean dregs, by 0.8-1.0 sieve, each raw material is carried out to ultramicro grinding, pulverizing dynamics is 100% mistake 20-40 order, then mix 150-300 second, at 100 ℃, granulate for the first time above, raw material gelatinization degree reaches more than 90%, obtains semi-finished product;
Low temperature is granulated for the second time: according to first throwing acidulant, amino acids, complex vitamin premix, composite mineral matter premix, throw in again tributyrin, complex enzyme formulation, probiotics, finally throw in sucrose, glucose, the order of zinc oxide and other raw material is carried out the input of small powder, with the semi-finished product mixing 150-300 second of pulverizing by 0.8-1.0 sieve, pulverizing dynamics is 100% mistake 20-40 order, at steam pressure, be 0.2-0.4Mpa, pelleting temperature is under 60 ℃ of following conditions, with 2.5-3.0 aperture ring film, carrying out low temperature for the second time granulates, finally by excessively broken, sieve, the cooling finished product that obtains.
Bacillus subtilis in the present invention (Bacillus subtilis) obtains for applicant's separation from traditional zymotic fermented soya bean, its biological property is as follows: individual cells, 0.7-0.8 * 2-3 μ m bacterium colony rough surface is opaque, dirty white or micro-yellow, bacterium colony is circular, edge indentation, gram-positive bacteria; Gemma 0.6-0.9 * 1.0-1.5 μ m, form is oval to column, is positioned at thalline central authorities or slightly inclined to one side, after sporulation, thalline does not expand, aerobic bacteria.Bacillus subtilis acid resistance in the present invention is strong, bile tolerance, high temperature resistant (can tolerate the pelleting temperature of 80 ℃), afterwards after entering animal intestinal, can keep the biologically active changing, effectively suppress enteropathogenic E．Coli K88, K99 and staphylococcus aureus, promote beneficial bacterium growth.Can synthesize the enzymes such as AMS, protease, lipase, cellulase by thalline self, in alimentary canal with animal body in digestive enzymes together play a role, can improve feed nutrient digestibility, improve breeding performonce fo animals, be conducive to reduce culture-cycle and aquaculture cost.Can synthetic vitamin the multiple B family vitamin such as B1, B2, B6, nicotinic acid, for the normal growth of animal body and the performance of physiological function provide safeguard, and improve the activity of interferon and macrophage in animal body, strengthen immunity function and anti-disease ability.Bacillus subtilis of the present invention (Bacillus subtilis) has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on September 4th, 2012, be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.6511.
Lactobacillus acidophilus in the present invention (Lactobacillus acidophilus) obtains for applicant's separation from animal intestinal, can secrete antibiotin class material (acidolin, bacillus acidophilus's element, lactein), pathogenic entero becteria is produced to antagonism, the propagation that suppresses enteron aisle undesirable microorganism, adjust gut flora balance, its biological property is as follows: bacillus, two terminal circle, 0.6-0.9 * 1.5-6 μ m, with single, become two or short chain to occur; Do not move, calot, bacterium colony is coarse.Examine under a microscope, show and be wound around or microvillus filiform, from stretching out like the radiation of shadow Jun Dui center.Without distinctive pigment.Amarogentin, cellobiose, aesculin, fructose, maltose, galactolipin, lactose, sucrose, mannose, melibiose, raffinose, trehalose can be assimilated, arabinose, gluconate, mannose can not be assimilated.Homofermentation, produces DL-LACTIC ACID.At 15 ℃, do not grow, optimum growth temperature is 35 ℃-38 ℃, and growth initial pH value is 5.0-7.0, and the most suitable growth pH value is 5.5-6.0.Lactobacillus acidophilus of the present invention (Lactobacillus acidophilus) applicant has been preserved in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms on September 4th, 2012, be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.6499.
The new bacterial strain of Pediococcus acidilactici provided by the invention (Pediococcus acidilactici) obtains for applicant's separation from duck jejunal content.Biological property is as follows: cell spherical shape, and at right angle, two planes alternately divide formation tetrad shape, and general cell is raw in pairs, and single survivor is rare, does not become catenation.Gram-positive, does not move, amphimicrobian.On MRS culture medium, bacterium colony is little, is white in color.Glucose, ribose, wood sugar, galactolipin, lactose, sucrose, trehalose, fructose, aesculin, mannose, rhamnose can be utilized, but Arabinose, maltose, sucrose etc. can not be utilized; Decomposing protein not, can not hydrolyzed starch, does not reduce nitrate, does not produce indoles; Catalase reaction negative, catalase is negative.Can grow containing in 6%～8% NaCl environment, resistance to NaCl concentration 13%～20%.Growth temperature 15-45 ℃, pH4.5-9.6, it is good that to be subacidity grow when neutral pH.The new bacterial strain HT of Pediococcus acidilactici (Pediococcus acidilactici) has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 09 04th, 2012, be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.6500.
Yue Shi lactobacillus of the present invention (Lactobacillus johnsonii) CGMCCNo.4926 is that applicant screens from the Radix Polygalae Crotalarioidis of Beijing, the domestication of process hydrochloric acid in gastric juice, cholate and seed selection obtain, this strain Yue Shi lactobacillus resistance is more intense, has good acid resistance, artificial gastro-intestinal Fluid tolerance and cholate tolerance.The center preservation of this bacterial strain Yi China Committee for Culture Collection of Microorganisms's common micro-organisms, preservation date is on June 8th, 2011, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, be called for short CGMCC, preserving number is CGMCC No.4926.
Saccharomyces cerevisiae of the present invention (Saccharomy cescerevisiae) bacterial strain separation is in fresh lichee skin, called after K1, and in 2012 09 year 11 days, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.6560.Saccharomyces cerevisiae K1 of the present invention compares with existing saccharomyces cerevisiae, and the common Gram-negative in chitling road and positive bacteria are all had to inhibitory action, therefore has unexpected technique effect.And this primary yeast is easily cultivated, after fermentation, processing, viable count is higher.
Creep feed feed formula of the present invention adopts Ideal Amino Acid Pattern, and net energy system is used the nutriment of multiple absorption easy to digest, adds synthesizing amino acid and other nutritive additive, meets the nutritional need of weanling pig and improves the physiological function of piggy.
In creep feed of the present invention, use FSPC: FSPC is to remove after water-soluble or the non-protein part of alcohol dissolubility with high-quality dregs of beans, the prepared 65%(butt that contains) soy protein products of above protein.Various amino acid contents and abundant, after animal food, digestibility is high, and ANFs is extremely low, and without other peculiar smell, mouthfeel is good.
In creep feed of the present invention, use fermented bean dregs: the enzyme by microorganism secretion in sweat is more than 3000 soya-bean polypeptides of molecular weight by the Partial Protein enzymolysis in dregs of beans.Macro-molecular protein in fermented bean dregs is degraded to polypeptide, little peptide in order, reduces the antigenicity of vegetable protein; In fermentation process, produce a large amount of lactic acid, protease, vitamin etc., product has genuine milk fragrance or distinctive fermenting aroma; Be rich in lactic acid bacteria, bacillus, the probioticses such as saccharomycete.
Preferably >=99%, zinc oxide has prevention sucking pig diarrhoea and promotes the function of growth for the zinc content of zinc oxide of the present invention, acts on effectively stablely, is natural prodcuts, has natural attribute, and the bacteriostasis of zinc oxide is not found drug resistance so far.
Freshness≤25% of SDPP of the present invention; IGg content >=16%.SDPP main nutrient composition is protein, fat, carbohydrate, calcium, phosphorus and some animals essential constant and trace quantity mineral substance element, protein content is more than 70%, digestibility is more than 93%, the digestibility of the mineral elements such as iron, phosphorus, magnesium is also higher, can improve the full price of raising poultry nutritive and the balance of daily ration.In SDPP, contain abundant immune substance, immunoglobulin content reaches the more than 16% of SDPP, also contain the immune substances such as a large amount of growth factors, interferon, lysozyme, these substances are by the complete absorption of piglet, can effectively strengthen pigling immunity, improve the resistance of piglet to disease, reduce diarrhea rate and the death rate of piglet.
In the present invention, tributyrin is the energy source material that maintains intestinal epithelial cell, and it can improve intestinal villi growth, reduces the crypts degree of depth; And zinc oxide can be by maintaining intestinal epithelial cell permeability and preventing that cause of disease material or antigen from avoiding its infringement to intestinal epithelial cell to epithelial suction-operated, both synergies can well promote weanling pig intestinal microecology healthy, immunity and the nutrient absorption ability of intestinal walls resistance pathogen have been increased, thereby reach the effect of substitute antibiotics, make piglet can spend smoothly ablactation stress, avoid having loose bowels, improve immunity and the premunition of sucking pig.
The probiotics adding in the present invention contains the profitable strains such as a large amount of lactic acid bacterias and saccharomycete, and butyric acid and glyceryl 3-monobutyrate that tributyrin decomposition produces all belong to organic acid, under the synergy with acidulant, regulate the structure of intestines and stomach microorganism species, promote the breeding growth of lactic acid bacteria, suppress and stop the breeding of the harmful bacterias such as Escherichia coli, clostruidium, salmonella, β hemolytic bacterioid, the quantity that increases the dominant microfloras such as Bacillus acidi lactici, preponderates beneficial bacterium quantity.Wherein the synergy of tributyrin and acidulant, probiotics can suppress the breeding of harmful bacteria, makes intestinal flora keep balance, thereby reaches the effect of substitute antibiotics.
Adopt the raw materials such as expanded soybean, popcorn, SDPP, steam fish meal, fermented bean dregs simultaneously, in the time of balanced nutritious, improve the high palatability of sucking pig and digestibility comprehensively, sucking pig is eaten on can be fast after wean.Add a high proportion of lactose, the low albumen whey powder of low albumen, meet the needs of sucking pig to lactose.Low albumen whey powder can provide a large amount of lactose, and in piglet alimentary canal, fermentation can produce a large amount of lactic acid, reduces pH value, helps newborn digestion, suppresses the growth of malignant bacteria, and this has major significance to piglet health; The high-quality lactalbumin containing in low albumen whey powder has high digestibility, good amino acid kenel in piggy body, without the advantage of ANFs; also contain albumin and globulin (haemocyanin); enteron aisle is had to positive impact equally; immunoglobulin (Ig) particularly; enteron aisle is had to protection effect, can resist Escherichia coli.In low albumen whey powder, also contain newborn peroxidating ferment and lactoferrin, there is the function of sterilization and bacterium processed.
Do not using under antibiotic prerequisite, creep feed of the present invention can help piglet to realize early weaning and smoothly by ablactation stress, regulate sucking pig intestines and stomach healthy, controls diarrhoea, improves survival rate, improves immunity and the premunition of sucking pig comprehensively; Employing amino acid balance is theoretical, selects easy to digestly, easily absorbs, and without the raw material of ANFs, when meeting sucking pig Fast Growth nutritional need, improves palatability and the digestibility of sucking pig comprehensively, guarantees sucking pig healthy growth.
Creep feed of the present invention is prepared as first primary raw material is carried out after scientific matching, granulate for the first time and make semi-finished product, pelleting temperature is more than 100 ℃, make its complete slaking, starch burn degree reaches more than 90%, not only the ANFs in raw material can be effectively removed, raw material palatability and digestibility can also be improved.
Low temperature is granulated for the second time, other raw material of other are functional and trophism is according to first throwing acidulant, amino acids, complex vitamin premix, composite mineral matter premix, throwing in tributyrin again, complex enzyme formulation, probiotics, the order of finally throwing in sucrose, glucose, zinc oxide and other auxiliary material claims to carry out the input of small powder with trace, the error that makes to feed intake is accurate to 0.01kg; After mixing with semi-finished product, low temperature is granulated again, and pelleting temperature is below 60 ℃.Low temperature is granulated and can be reduced the nutritive loss of heat sensitivity raw material for the second time, feeding sequence can guarantee that this alkaline matter of zinc oxide does not react with other acidic materials generation acid-base neutralizations such as acidulants, make each nutritional labeling reach maximized effectiveness, finished product after secondary low temperature is granulated can reach the instant effect of 3s, and palatability is better.
The specific embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Embodiment 1 bacillus subtilis bacterium powder preparation
1, the preparation of bacillus subtilis (Bacillus subtilis) CGMCC No.6511 active bacteria formulation
1) preparation of bacillus subtilis seed liquor: test tube slant preservation of bacteria strain is seeded in the test tube that 15mL culture medium is housed, cultivates 16-20h in 37 ℃ of constant-temperature tables, rotating speed is 225rpm, treats that viable count reaches 10
7cFU/mL, is inoculated in the 250mL conical flask that 100mL seed culture medium is housed by 1%, in 37 ℃ of constant-temperature tables, cultivates 16-20h, and shaking speed is 225rpm, treats that viable count reaches 10
7cFU/mL, standby as seed liquor.
Seed culture medium is: glucose, 20.0g/L; Peptone, 10.0g/L; Dusty yeast, 4.0g/L; Sodium chloride, 5g/L; Distilled water adds to 1000mL, pH7.0 ± 0.2.
2) liquid deep layer fermenting is cultivated
Select 5L vertical digester to carry out liquid fermentation and culture.By the inoculum concentration of bacillus subtilis seed liquor, be 1%, after 20%, 37 ℃ of aerobic cultivation 20h of liquid amount, measure every milliliter of viable count in bacterium liquid, treat that viable count reaches 10
8more than CFU/mL, spore forming rate arrives 80%, and warping finishes fermented and cultured, and (defoamer is vegetable oil, addition 1%， unit: g/L).
Fermentation medium is composed as follows: peptone, 50g/L; Glucose 10g/L; Dusty yeast 17g/L; Compound sugar 3g/L; Distilled water adds to 1000mL, pH7.0 ± 0.2.
3) freeze-dried vaccine powder preparation
By zymotic fluid centrifugal, concentrate, add the production routines such as freeze drying protectant, vacuum freeze drying and the check of bacterium powder viable count, finally prepare powdery active bacteria formulation.
4) finished product detection
Get the international NY/T 1461-2007 of the powdery active bacteria formulation 1g，An method of having prepared and measure the viable count in every gram of bacterium powder, record viable count in bacterium powder and be greater than 10
8cFU/g.
The preparation of embodiment 2 lactobacillus acidophilus bacterium powder
1) preparation of lactobacillus acidophilus CGMCC No.6499 seed liquor: preservation of bacteria strain lactobacillus acidophilus in glycerine cryopreservation tube is rule in MRS agar medium flat board with transfer needle, 37 ℃ of constant temperature culture 20h.Then by the single colony inoculation growing on flat board to being equipped with in the 250mL conical flask of 100mLMRS fluid nutrient medium, 37 ℃ of constant temperature culture 20h.The cultivation of again cultured bacterium liquid being rule on MRS culture medium flat plate, after 37 ℃ of constant temperature culture 20h, by single colony inoculation to being equipped with in the 250mL conical flask of 100mLMRS fluid nutrient medium, 37 ℃ of constant temperature culture 20h.To operate above repetition 3 times, complete the activation of lactobacillus acidophilus.
The preparation of lactobacillus acidophilus seed liquor: the lactobacillus acidophilus bacterium liquid having activated is added to 5L fermentation tank with 5% inoculum concentration.Culture parameters is: temperature, 37 ℃; Mixing speed, 50rmp; PH value, 6.0 ± 0.5; Incubation time, 24h.The CaCO that adds 0.5-1% in sweat
3, regulate pH 6.2～6.5; After fermentation 8h, carry out feed supplement, add 50% glucose 200mL.
Above-mentioned lactobacillus acidophilus liquid seeds liquid is joined in 50L fermentation tank with 1% inoculum concentration, and culture parameters is: temperature, 37 ℃; Mixing speed, 50rmp; PH value, 6.0 ± 0.5; Incubation time, 20h.The CaCO that adds 0.5-1% in sweat
3, regulate pH 6.2～6.5; After fermentation 8h, carry out feed supplement, add 50% glucose 1L.
Cultivate lactobacillus acidophilus MRS Liquid Culture based formulas used as follows: peptone, 10.0g/L; Beef extract, 8.0g/L; Yeast extract, 4.0g/L; Glucose, 20.0g/L; Tween 80,1mL/L; Dipotassium hydrogen phosphate, 2.0g/L; Sodium acetate trihydrate, 5.0g/L; Triammonium citrate, 2.0g/L; Epsom salt, 0.2g/L; Manganese sulfate, 0.05g/L; PH6.2 ± 0.2.
MRS agar medium is on the basis of MRS fluid nutrient medium, to add agar 15g/L.
It is as follows that 50L fermentation tank propagation is cultivated lactobacillus acidophilus Optimal Medium formula used: peptone, 10.0g/L; Beef extract, 8.0g/L; Yeast extract, 6.0g/L; Glucose, 25.0g/L; Tween 80,1mL/L; Dipotassium hydrogen phosphate, 3.0g/L; Sodium acetate trihydrate, 5.0g/L; Triammonium citrate, 2.0g/L; Epsom salt, 0.2g/L; Manganese sulfate, 0.05g/L; PH6.2 ± 0.2.
3) freeze-dried vaccine powder preparation
By zymotic fluid centrifugal, concentrate, add the production routines such as freeze drying protectant, vacuum freeze drying and the check of bacterium powder viable count, finally prepare powdery active bacteria formulation.
4) finished product detection
Get the international GB/T 4789.35-2003 of the powdery active bacteria formulation 1g，An method of having prepared and measure the viable count in every gram of bacterium powder, and in bacterium powder, viable count is greater than 10
8cFU/g.
The preparation of embodiment 3 Pediococcus acidilactici bacterium powder
1) preparation of Pediococcus acidilactici CGMCC No.6500 seed liquor
Test tube slant preservation of bacteria strain is seeded in the anaerobism pipe that 20mL MRS fluid nutrient medium is housed, 37 ℃ of constant temperature anaerobism are cultivated 20h, treat that viable count reaches 10
7cFU/mL, is inoculated in the 250mL conical flask that 100mL MRS fluid nutrient medium is housed by 1%, and 37 ℃ of anaerobism are cultivated 20h, treat that viable count reaches 10
7cFU/mL(MRS agar medium plate count) more than, standby as seed liquor.
2) liquid deep layer fermenting is cultivated
Above-mentioned Pediococcus acidilactici liquid seeds liquid is joined in 50L fermentation tank with 1% inoculum concentration, and culture parameters is: temperature, 37 ℃; Mixing speed, 50rmp; PH value, 6.0 ± 0.5; Incubation time, 20h.The CaCO that adds the cumulative volume 0.5% that ferments in sweat
3, regulate pH at 6.2-6.5; After fermentation 8h, carry out feed supplement, add 50% glucose 1L.
Cultivate Pediococcus acidilactici MRS Liquid Culture based formulas used as follows: peptone, 10.0g/L; Beef extract, 8.0g/L; Yeast extract, 4.0g/L; Glucose, 20.0g/L; Tween 80,1mL/L; Dipotassium hydrogen phosphate, 2.0g/L; Sodium acetate trihydrate, 5.0g/L; Triammonium citrate, 2.0g/L; Epsom salt, 0.2g/L; Manganese sulfate, 0.05g/L; PH6.2 ± 0.2.
MRS agar medium is on the basis of MRS fluid nutrient medium, to add agar 15g/L.
It is as follows that 50L fermentation tank propagation is cultivated Pediococcus acidilactici Optimal Medium formula used: peptone, 10.0g/L; Beef extract, 8.0g/L; Yeast extract, 6.0g/L; Glucose, 25.0g/L; Tween 80,1mL/L; Dipotassium hydrogen phosphate, 3.0g/L; Sodium acetate trihydrate, 5.0g/L; Triammonium citrate, 2.0g/L; Epsom salt, 0.2g/L; Manganese sulfate, 0.05g/L; PH6.2 ± 0.2.
3) freeze-dried vaccine powder preparation
By zymotic fluid centrifugal, concentrate, add the production routines such as freeze drying protectant (containing 5% trehalose, 15% skimmed milk power, 4% sucrose, 1%D-arabo-ascorbic acid and 2.0% sodium glutamate), vacuum freeze drying and the check of bacterium powder viable count, finally prepare powdery active bacteria formulation.
4) finished product detection
Get the international GB/T4789.35-2003 method of the powdery active bacteria formulation 1g，An having prepared and measure the viable count in every gram of bacterium powder, and in bacterium powder, viable count is greater than 10
8cFU/g.
The preparation of embodiment 4 Yue Shi lactobacillus CGMCC No.4926 bacterium powder
The preparation of Yue Shi lactobacillus seed liquid: test tube slant preservation of bacteria strain is seeded in the anaerobism pipe that 20mL MRS culture medium is housed, 37 ℃ of constant temperature anaerobism are cultivated 20h, treat that viable count reaches 10
7cFU/mL, is inoculated in the 250mL conical flask that 100mL seed culture medium is housed by 1%, and 37 ℃ of anaerobism are cultivated 20h, treat that viable count reaches 10
7more than CFU/mL, standby as seed liquor.
Seed liquor MRS culture medium is: peptone, 10.0g/L; Beef extract, 8.0g/L; Yeast extract, 4.0g/L; Glucose, 20.0g/L; Tween 80,1mL/L; Dipotassium hydrogen phosphate, 2.0g/L; Sodium acetate trihydrate, 5.0g/L; Triammonium citrate, 2.0g/L; Epsom salt, 0.2g/L; Manganese sulfate, 0.05g/L; Distilled water adds to 1000mL, pH6.2 ± 0.2.
Liquid deep layer fermenting is cultivated
Select 100L vertical digester to carry out liquid fermentation and culture.Wherein, the inoculum concentration of Yue Shi lactobacillus seed liquid is 1%, and 65%, 37 ℃ of anaerobism of liquid amount is cultivated after 20h, measures every milliliter of viable count in bacterium liquid, treats that viable count reaches 10
8more than CFU/mL, warping finishes fermented and cultured.
Fermentation medium is composed as follows: soy peptone 50g, and glucose 10g, dusty yeast 17g, compound sugar 3g, distilled water adds to 1000mL, pH6.2 ± 0.2.
The preparation of freeze-dried vaccine powder
By zymotic fluid centrifugal, concentrate, add the production routines such as freeze drying protectant, vacuum freeze drying and the check of bacterium powder viable count, finally prepare powdery active bacteria formulation.Wherein, freeze drying protectant composed as follows: sucrose 15g, sorbierite 15g, skimmed milk power 18g, distilled water adds to 250mL; Bacterium mud (thalline after centrifugal concentrating) is 1: 125 (g/mL) with the adding proportion of freeze drying protectant, and frozen-dried protective rate reaches more than 72%.
Finished product detection
Get the international GB/T4789.35-2003 method of the powdery active bacteria formulation 1g，An having prepared and measure the viable count in every gram of bacterium powder, and in bacterium powder, viable count is greater than 10
8cFU/g.
The preparation of embodiment 5 saccharomyces cerevisiae CGMCC No.6560 bacterium powder
1) dull and stereotyped cultivation rejuvenation: the saccharomyces cerevisiae slant strains of refrigerator preservation is adopted to method streak inoculation on No. 98 plating mediums of streak inoculation, in 30 ℃ of cultivation 46h, make saccharomyces cerevisiae rejuvenation, and form single bacterium colony; Picking list bacterium colony, on No. 98 fresh slant mediums, is cultivated 36h ， Fang Ge refrigerator in 30 ℃ standby;
2) preparation of first order seed: the fresh slant strains of the cultured saccharomyces cerevisiae of step 1) is transferred in the 500mL triangular flask that 200ml98 culture medium is housed, and in 30 ℃ of cultivation 12～16h, rotating speed 150rpm, makes it in the logarithm middle and later periods, is first order seed;
3) preparation of secondary seed: by step 2) cultured saccharomyces cerevisiae seed liquor is transferred in the 2.0L triangular flask that 1.2L98 culture medium is housed, and in 30 ℃ of static cultivation 12h, is secondary seed solution;
4) preparation of zymotic fluid: secondary seed prepared by step 3) is inoculated into 15～16M is housed according to 10% inoculum concentration
3in the fermentation tank of fermentation medium, 30 ℃ of temperature, rotating speed 200rpm, tank pressure 0.05Mpa, cultivates 16h stuck fermentation, and now viable count is 1.5 * 10
9cfu/ml;
Described fermentation medium is: brown sugar 1%, soy peptone 1%, yeast extract 0.2%, sodium chloride 0.5%, magnesium sulfate 0.01%, dipotassium hydrogen phosphate 0.2%.
5) preparation of S. cervisiae powder: zymotic fluid prepared by step 4), 2000pm is centrifugal, obtains bacterium mud; add the freeze drying protectant that the weight/volume percent with bacterium mud is 10～15%; after mixing, in-25 ℃ of freeze dryings, obtain moisture <5%, viable count>=10
9the S. cervisiae powder of cfu/g;
Described freeze drying protectant be skimmed milk power, glycerine, maltodextrin according to: 2:0.5:1 ratio mixes.
Embodiment 6 nonreactive piglet preweaning feeds
The raw material of granulating for the first time: 36.4 parts of popcorns, 22 parts of expanded soybeans, 5 parts of steam fish meal, 2 parts of FSPCs, 3 parts of fermented bean dregs;
The raw material of granulating for the second time: 3 parts of SDPPs, 13 parts of low albumen whey powders, 8 parts of lactose, 2 parts of glucose, 2.5 parts of sucrose, 0.2 part of salt, 0.05 part of antioxidant, 0.3 part of lysine, 0.3 part of threonine, 0.3 part of methionine, 0.1 part of tryptophan, 0.25 part, zinc oxide, 0.35 part of tributyrin, 0.2 part of probiotics, 0.3 part of (pig treasured 500 of compound acidulant, purchased from Shanghai Niu Cuite feed technology Co., Ltd, this product is mainly by fumaric acid, malic acid, citric acid and phosphoric acid form, adopt vegetable fat to be coated with), 0.15 part of complex enzyme formulation (challenge group), 0.3 part of complex vitamin premix, 0.3 part of composite mineral matter premix.
Probiotics: the bacterium powder of embodiment 1～5 preparation is mixed to total effective viable bacteria content>=1 * 10 in preparation by weight 1:1:1:1
9cFU/g.
Complex vitamin premix: contain 4,000 ten thousand IU vitamin As, 5,000,000 IU vitamin Ds in every 1kg complex vitamin premix
3, 35g vitamin E, 8500mg vitamin K, 6g Cobastab
1, 18g Cobastab
2, 10g vitamin B6,50mg Cobastab
12, 1500mg folic acid and 1500mg biotin, carrier is time powder and medical stone powder.
Composite mineral matter premix: in the premix of every 1kg composite mineral matter,, containing 35000mg copper, 40000mg iron, 15000mg manganese, 26000mg zinc, 100mg selenium, 120mg iodine, carrier is stone flour.
Granulate for the first time: by aforesaid popcorn, expanded soybean, steam fish meal, FSPC, fermented bean dregs, by 0.8 sieve, each raw material is pulverized, pulverizing dynamics is 100% mistake 40 orders, then mix 300 seconds, at 100 ℃, granulate for the first time above, obtain semi-finished product, raw material gelatinization degree reaches more than 90%.
Low temperature is granulated for the second time: according to first throwing acidulant, amino acids, complex vitamin premix, composite mineral matter premix, throw in again tributyrin, complex enzyme formulation, probiotics, finally throw in sucrose, glucose, the order of zinc oxide and other auxiliary material is thrown in aforementioned surplus stock, the error that makes to feed intake is accurate to 0.01kg, with with the semi-finished product that 0.9 sieve is pulverized, mix 300 seconds, pulverizing dynamics is 100% mistake 30 orders, at steam pressure, be 0.4Mpa, pelleting temperature is under 60 ℃ of following conditions, with 3.0 aperture ring films, carrying out low temperature for the second time granulates, finally by excessively broken, sieve, the cooling finished product that obtains.
Embodiment 7 nonreactive piglet preweaning feeds
The raw material of granulating for the first time: 45.79 parts of popcorns, 18 parts of expanded soybeans, 3 parts of steam fish meal, 3 parts of FSPCs, 4 parts of fermented bean dregs;
The raw material of granulating for the second time: 2 parts of SDPPs, 11 parts of low albumen whey powders, 5 parts of lactose, 3.5 parts of glucose, 3 parts of sucrose, 0.1 part of salt, 0.1 part of antioxidant, 98 0.15 part of lysine, 0.2 part of threonine, 0.2 part of methionine, 0.01 part of tryptophan, 0.1 part, zinc oxide, 0.1 part of tributyrin, 0.15 part of probiotics, 0.15 part of (pig treasured 500 of compound acidulant, purchased from Shanghai Niu Cuite feed technology Co., Ltd, this product is mainly by fumaric acid, malic acid, citric acid and phosphoric acid form, adopt vegetable fat to be coated with), 0.2 part of complex enzyme formulation (challenge group), 0.15 part of complex vitamin premix, 0.1 part of composite mineral matter premix.
Probiotics: the bacterium powder of embodiment 1～5 preparation is mixed to total effective viable bacteria content>=1 * 10 in preparation by weight 1:1:1:1
9cFU/g.
Complex vitamin premix: contain 4,000 ten thousand IU vitamin As, 5,000,000 IU vitamin Ds in every 1kg complex vitamin premix
3, 35g vitamin E, 8500mg vitamin K, 6g Cobastab
1, 18g Cobastab
2, 10g vitamin B6,50mg Cobastab
12, 1500mg folic acid and 1500mg biotin, carrier is time powder and medical stone powder.
Composite mineral matter premix: in the premix of every 1kg composite mineral matter,, containing 35000mg copper, 40000mg iron, 15000mg manganese, 26000mg zinc, 100mg selenium, 120mg iodine, carrier is stone flour.
Granulate for the first time: by aforesaid popcorn, expanded soybean, steam fish meal, FSPC, fermented bean dregs, by 1.0 sieves, each raw material is pulverized, pulverizing dynamics is 100% mistake 40 orders, then mix 300 seconds, at 100 ℃, granulate for the first time above, obtain semi-finished product, raw material gelatinization degree reaches more than 90%.
Low temperature is granulated for the second time: according to first throwing acidulant, amino acids, complex vitamin premix, composite mineral matter premix, throw in again tributyrin, complex enzyme formulation, probiotics, finally throw in sucrose, glucose, the order of zinc oxide and other auxiliary material is thrown in aforementioned surplus stock, the error that makes to feed intake is accurate to 0.01kg, with with the semi-finished product that 0.8 sieve is pulverized, mix 300 seconds, pulverizing dynamics is 100% mistake 20 orders, at steam pressure, be 0.2Mpa, pelleting temperature is under 60 ℃ of following conditions, with 3.0 aperture ring films, carrying out low temperature for the second time granulates, finally by excessively broken, sieve, the cooling finished product that obtains.
Embodiment 8 nonreactive piglet preweaning feeds
The raw material of granulating for the first time: 39.5 parts of popcorns, 20.5 parts of expanded soybeans, 4 parts of steam fish meal, 3 parts of FSPCs, 2 parts of fermented bean dregs;
The raw material of granulating for the second time: 3 parts of SDPPs, 13 parts of low albumen whey powders, 7 parts of lactose, 4 parts of glucose, 2 parts of sucrose, 0.1 part of salt, 0.1 part of antioxidant, 98 0.15 part of lysine, 0.1 part of threonine, 0.2 part of methionine, 0.05 part of tryptophan, 0.25 part, zinc oxide, 0.25 part of tributyrin, 0.1 part of probiotics, 0.2 part of (pig treasured 500 of compound acidulant, purchased from Shanghai Niu Cuite feed technology Co., Ltd, this product is mainly by fumaric acid, malic acid, citric acid and phosphoric acid form, adopt vegetable fat to be coated with), 0.1 part of complex enzyme formulation (challenge group), 0.2 part of complex vitamin premix, 0.2 part of composite mineral matter premix.
Probiotics: the bacterium powder of embodiment 1～5 preparation is mixed to total effective viable bacteria content>=1 * 10 in preparation by weight 1:1:1:1
9cFU/g.
Complex vitamin premix: contain 4,000 ten thousand IU vitamin As, 5,000,000 IU vitamin Ds in every 1kg complex vitamin premix
3, 35g vitamin E, 8500mg vitamin K, 6g Cobastab
1, 18g Cobastab
2, 10g vitamin B6,50mg Cobastab
12, 1500mg folic acid and 1500mg biotin, carrier is time powder and medical stone powder.
Composite mineral matter premix: in the premix of every 1kg composite mineral matter,, containing 35000mg copper, 40000mg iron, 15000mg manganese, 26000mg zinc, 100mg selenium, 120mg iodine, carrier is stone flour.
Granulate for the first time: by aforesaid popcorn, expanded soybean, steam fish meal, FSPC, fermented bean dregs, by 0.8 sieve, each raw material is pulverized, pulverizing dynamics is 100% mistake 20 orders, then mix 300 seconds, at 100 ℃, granulate for the first time above, obtain semi-finished product, raw material gelatinization degree reaches more than 90%.
Low temperature is granulated for the second time: according to first throwing acidulant, amino acids, complex vitamin premix, composite mineral matter premix, throw in again tributyrin, complex enzyme formulation, probiotics, finally throw in sucrose, glucose, the order of zinc oxide and other auxiliary material is thrown in aforementioned surplus stock, the error that makes to feed intake is accurate to 0.01kg, with with the semi-finished product that 0.8 sieve is pulverized, mix 300 seconds, pulverizing dynamics is 100% mistake 20 orders, at steam pressure, be 0.2Mpa, pelleting temperature is under 60 ℃ of following conditions, with 2.5 aperture ring films, carrying out low temperature for the second time granulates, finally by excessively broken, sieve, the cooling finished product that obtains.
Test example 1
The present invention is applicable to the high-grade piglet preweaning feed of nonreactive of early weaned piglets, through assay, the guarantee value of its nutritional labeling is crude protein >=18, coarse ash≤8.0, crude fibre≤4.0, calcium 0.3-1.3, total phosphorus >=0.3, lysine >=1.5, moisture≤14.0, salt 0.3-1.3.
Test example 2
This test selects the sodium selenite of 160 wean in 28 days as experimental animal, observes the impact of the high-grade piglet preweaning feed of nonreactive on weaned piglets after feeding two weeks.We are divided into four groups at random by 160 weanling pigs, every group of four repetitions, control group, test 1 group, test 2 groups and test 3 groups.Wherein control group fed tradition is containing antibiotic creep feed daily ration, and nonreactive creep feed daily ration in 1 group of test example, 2 groups of test examples and test example 3 groups of above-described embodiments 6 of feeding respectively, embodiment 7 and embodiment 8, other experimental condition is all consistent.Time-division on-test another name is got the initial body weight of every pig, and pig free choice feeding every day and drinking-water, feed after two weeks according to experimental design, then take respectively the end body weight of every pig.Duration of test records the feed intake of every group of pig every day, simultaneously survival rate and the diarrhea rate of observed and recorded weanling pig.With SAS9.0, carry out data analysis, result all represents with average ± standard deviation.
Average daily ingestion amount (AFI)=feed intake/test number of days
| ? | Control group | Test 1 group | Test 2 groups | Test 3 groups | P |
| Initial body weight (kg) | 6.83±1.36 | 6.45±0.23 | 6.57±1.74 | 6.12±1.45 | P=0.08 |
| Finish body weight (kg) | 11.03±0.18 | 11.21±2.12 | 11.64±0.85 | 12.27±1.62 | P=0.12 |
| Average daily ingestion amount (g) | 394±45 | 416±60 | 434±40 | 490±33 | P=0.03 |
| Average daily gain (g) | 300±32 | 340±34 | 362±21 | 439±37 | P=0.02 |
| Feedstuff-meat ratio | 1.31±0.04 | 1.22±0.10 | 1.20±0.08 | 1.12±0.07 | P=0.02 |
| Survival rate (%) | 97.5% | 97.5% | 100% | 100% | ? |
| Diarrhea rate (%) | 12.5% | 7.5% | 5% | 2.5% | ? |
Result of the test is as follows:
Test 1 group, test 2 groups and average daily ingestion amount and the average daily gain of testing sucking pig in 3 groups and be all significantly higher than control group (p < 0.05); Wherein test 3 groups and be significantly higher than 1 group of test and 2 groups (p < 0.05) of test.Test 1 group, test 2 groups and the feedstuff-meat ratio of testing sucking pig in 3 groups all work lower than control group (p < 0.05); Wherein test 3 groups significantly lower than 1 group of test example and 2 groups of test examples (p < 0.05).Test in 3 groups of 2 groups and tests the survival rate of sucking pig higher than control group; Test 1 group, test 2 groups and the diarrhea rate of testing sucking pig in 3 groups lower than control group.
Interpretation of result: we can find out 1 group of the test of searching for food, test 2 groups and the production performance of testing the religion of nonreactive in 3 groups groove daily ration piglet and will be significantly higher than the tradition of searching for food and have anti-group from result of the test; And the production performance of testing 3 groups of piglets is best, the formula that embodiment 8 is described is optimum formula combination.
Test example 3
This test by the system of granulating by secondary low temperature the high-grade piglet preweaning feed of nonreactive and the mode system of once granulating through tradition the high-grade piglet preweaning feed of nonreactive carry out contrast test.We are divided into two groups at random by 90 28 age in days weanling pigs, every group of three repetitions, i.e. once granulation group and secondary granulation group.Once granulation group is fed and is adopted the high-grade piglet preweaning feed of nonreactive of traditional disposable granulating process processing, the high-grade piglet preweaning feed of nonreactive that secondary granulation group is fed and adopted secondary low temperature granulating process to process.Test method is with test example 2, and its result is as follows:
| ? | Once granulation group | Secondary granulation group | P |
| Initial body weight (kg) | 6.67±1.24 | 6.54±0.13 | P=0.11 |
| Finish body weight (kg) | 11.12±2.31 | 12.43±1.42 | P=0.09 |
| Average daily ingestion amount (g) | 410±36 | 484±46 | P=0.01 |
| Average daily gain (g) | 318±41 | 421±50 | P=0.02 |
| Feedstuff-meat ratio | 1.29±0.08 | 1.15±0.07 | P=0.21 |
| Survival rate (%) | 97.5% | 100% | ? |
| Diarrhea rate (%) | 6.7% | 2.2% | ? |
Result of the test is as follows:
The average daily ingestion amount of secondary granulation group sucking pig and the weight average that increases day by day are significantly greater than once granulation group (p < 0.05).The feedstuff-meat ratio of the sucking pig of secondary granulation group will be significantly lower than once granulation group (p < 0.05).The survival rate of secondary granulation group sucking pig will be higher than once granulation group and diarrhea rate lower than once granulation group.
Interpretation of result: adopt the high-grade sucking pig material of nonreactive of secondary low temperature granulation processing than adopting the high-grade piglet preweaning feed of nonreactive of traditional processing of once granulating to have better production performance, daily gain and survival rate are higher, and feedstuff-meat ratio and diarrhea rate are lower.Because adopt the processing of secondary low temperature granulating process can make feedstuff gelatinization rate reach more than 90% time can also guarantee to reduce the nutritive loss of heat sensitivity raw material, make each nutritional labeling reach maximized effectiveness, palatability is better, trophism and digestibility higher, sucking pig feed intake is large, therefore can reach best feeding effect.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (9)
1. a nonreactive piglet preweaning feed, it is characterized in that: it comprises granulate for the first time raw material and the raw material of granulating for the second time, wherein, the described raw material of granulating for the first time comprises the component of following weight portion: popcorn 35-50 part, expanded soybean 15-22 part, steam fish meal 3-5 part, FSPC 2-5 part and fermented bean dregs 1-4 part, the described raw material of granulating for the second time comprises the component of following weight portion: SDPP 2-4 part, low albumen whey powder 10-15 part, lactose 5-10 part, glucose 3-5 part, sucrose 2-5 part, salt 0.1-0.3 part, antioxidant 0.05-0.1 part, lysine 0.1-0.3 part, threonine 0.1-0.3 part, methionine 0.1-0.3 part, tryptophan 0.01-0.1 part, zinc oxide 0.1-0.3 part, tributyrin 0.1-0.4 part, probiotics 0.05-0.2 part, compound acidulant 0.1-0.4 part, complex enzyme formulation 0.05-0.2 part, complex vitamin premix 0.1-0.3 part, composite mineral matter premix 0.1-0.3 part.
2. nonreactive piglet preweaning feed according to claim 1, is characterized in that: described complex enzyme formulation comprises amylase 1300U/g, protease 2500U/g, cellulase 1500U/g, pectase 2000U/g, phytase 50U/g and 1,4 beta-glucanase 3500U/g.
3. nonreactive piglet preweaning feed according to claim 1, is characterized in that: described probiotics comprises bacillus subtilis, lactobacillus acidophilus, Pediococcus acidilactici, Yue Shi lactobacillus and S. cervisiae total effective viable bacteria content>=1 * 10
9cFU/g.
4. nonreactive piglet preweaning feed as claimed in claim 1, it is characterized in that: described bacillus subtilis is bacillus subtilis CGMCC No.6511, described lactobacillus acidophilus is lactobacillus acidophilus CGMCC No.6499, described Pediococcus acidilactici is Pediococcus acidilactici CGMCC No.6500, described Yue Shi lactobacillus is Yue Shi lactobacillus CGMCC No.4926, and described S. cervisiae is S. cervisiae No.6560.
5. nonreactive piglet preweaning feed according to claim 1, is characterized in that: described acidulant is mainly comprised of fumaric acid, malic acid, citric acid and phosphoric acid, adopts vegetable fat coated.
6. nonreactive piglet preweaning feed according to claim 1, is characterized in that: described antioxidant is one or more of L-AA, Butylated Hydroxytoluene, fourth hydroxyl methoxy benzene, tocopherol, ethoxyquinoline etc.
7. nonreactive piglet preweaning feed according to claim 1, is characterized in that: in the premix of every 1kg composite mineral matter, containing 35000mg copper, 40000mg iron, 15000mg manganese, 26000mg zinc, 100mg selenium, 120mg iodine, carrier is stone flour.
8. nonreactive piglet preweaning feed according to claim 1, is characterized in that: in every 1kg complex vitamin premix, contain 4,000 ten thousand IU vitamin As, 5,000,000 IU vitamin Ds
3, 35g vitamin E, 8500mg vitamin K, 6g Cobastab
1, 18g Cobastab
2, 10g vitamin B6,50mg Cobastab
12, 1500mg folic acid and 1500mg biotin, carrier is time powder and medical stone powder.
9. the preparation method of the nonreactive piglet preweaning feed described in claim 1～8 any one, it comprises the steps:
Granulate for the first time: take by weight popcorn, expanded soybean, steam fish meal, FSPC, fermented bean dregs, by 0.8-1.0 sieve, each raw material is carried out to ultramicro grinding, pulverizing dynamics is 100% mistake 20-40 order, then mix 150-300 second, at 100 ℃, granulate for the first time above, raw material gelatinization degree reaches more than 90%, obtains semi-finished product;
Low temperature is granulated for the second time: according to first throwing acidulant, amino acids, complex vitamin premix, composite mineral matter premix, throw in again tributyrin, complex enzyme formulation, probiotics, finally throw in sucrose, glucose, the order of zinc oxide and other raw material is carried out the input of small powder, with the semi-finished product mixing 150-300 second of pulverizing by 0.8-1.0 sieve, pulverizing dynamics is 100% mistake 20-40 order, at steam pressure, be 0.2-0.4Mpa, pelleting temperature is under 60 ℃ of following conditions, with 2.5-3.0 aperture ring film, carrying out low temperature for the second time granulates, finally by excessively broken, sieve, the cooling finished product that obtains.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6217915B1 (en) * | 1998-10-14 | 2001-04-17 | Wisconsin Alumni Research Foundation | Feed additive that increases availability of butyric acid and non-digestible oligosaccharides in the G.I. tract |
| CN1513347A (en) * | 2003-07-24 | 2004-07-21 | 侯永清 | Application os glycerin tributyrate as feed additive |
| CN101181019A (en) * | 2007-12-13 | 2008-05-21 | 济南和美华饲料有限公司 | Feedstuff for pigling replacing milk |
| CN101390576A (en) * | 2007-09-17 | 2009-03-25 | 河南商都生物技术股份有限公司 | Production method of Anthony pig granule feedstuff |
| CN102356828A (en) * | 2011-10-31 | 2012-02-22 | 湖南创新生物科技有限公司 | Piglet early-stage microbial fermented antibiotic-free feed |
-
2013
- 2013-10-16 CN CN201310484499.3A patent/CN103598447B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6217915B1 (en) * | 1998-10-14 | 2001-04-17 | Wisconsin Alumni Research Foundation | Feed additive that increases availability of butyric acid and non-digestible oligosaccharides in the G.I. tract |
| CN1513347A (en) * | 2003-07-24 | 2004-07-21 | 侯永清 | Application os glycerin tributyrate as feed additive |
| CN101390576A (en) * | 2007-09-17 | 2009-03-25 | 河南商都生物技术股份有限公司 | Production method of Anthony pig granule feedstuff |
| CN101181019A (en) * | 2007-12-13 | 2008-05-21 | 济南和美华饲料有限公司 | Feedstuff for pigling replacing milk |
| CN102356828A (en) * | 2011-10-31 | 2012-02-22 | 湖南创新生物科技有限公司 | Piglet early-stage microbial fermented antibiotic-free feed |
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