CN110810625A - Waste mushroom stick biological fermentation feed and preparation method thereof - Google Patents
Waste mushroom stick biological fermentation feed and preparation method thereof Download PDFInfo
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- CN110810625A CN110810625A CN201911170957.XA CN201911170957A CN110810625A CN 110810625 A CN110810625 A CN 110810625A CN 201911170957 A CN201911170957 A CN 201911170957A CN 110810625 A CN110810625 A CN 110810625A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Inorganic Chemistry (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to a biological fermentation feed of waste mushroom sticks and a preparation method thereof, the biological fermentation feed of the waste mushroom sticks takes the waste mushroom sticks as main raw materials, and adding corn flour, bean pulp, rice bran, vegetables, beet sugar residue, soybean probiotic preparation, fructus crataegi, herba Portulacae, radix Acanthopanacis Senticosi, herba Taraxaci and herba plantaginis, fermenting, the feed fully utilizes the components of abundant mycelium, protein, amino acid, vitamin, mineral substances and the like contained in the waste mushroom sticks, the medicinal effects of the hawthorn, the purslane, the acanthopanax, the dandelion and the plantain are exerted, the appetite of livestock is increased, the disease resistance of the livestock is improved, and finally the fermented feed has rich nutrition, sour and fragrant taste and good palatability, the feed has the characteristics of rich mycoprotein, high absorption conversion rate, high quality, harmlessness, low cost, improvement on the quality of cultured products and the like; the feed effectively utilizes agricultural wastes such as waste mushroom sticks and the like, is favorable for the development of circular agriculture, and reduces the breeding cost.
Description
Technical Field
The invention belongs to the technical field of feeds, and particularly relates to a waste mushroom stick biological fermentation feed and a preparation method thereof.
Background
In recent years, with the development of edible fungi, the fungus dregs of the edible fungi, namely the waste materials after the edible fungi are cultivated, are more and more, millions of tons of fungus dregs are produced in China every year, and the fungus dregs are considerable resources. At present, except a small part of the fungus residues of the edible fungi are used for fertilizers, most of the fungus residues are usually discarded or combusted anywhere, so that the great waste of resources is caused, air, water sources and environment are polluted, and even haze is caused.
The biological fermentation feed takes microorganism and complex enzyme as biological feed starter strains, and the feed raw materials are converted into microbial mycoprotein, bioactive small peptide amino acid, microbial active probiotics and complex enzyme preparation as an integrated biological fermentation feed. The product not only can compensate amino acid which is easy to be lacked in conventional feed, but also can quickly convert nutrient components of other raw feed materials, thereby achieving the effect of enhancing digestion, absorption and utilization. Meanwhile, the biological fermentation feed contains a large amount of amino acid, mycoprotein, probiotics and the like, the immunity of animals can be enhanced after the animals eat the biological fermentation feed, the use of antibiotics is reduced, the quality of cultured products is improved, and the comprehensive effect of the biological fermentation feed shows the application advantages and very good market prospect of the biological fermentation feed in the aspect of culture.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides the waste mushroom-stick biological fermentation feed and the preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a biological fermentation feed of waste mushroom sticks is prepared from the following raw materials in parts by weight: 20-40 parts of waste mushroom sticks, 5-15 parts of corn flour, 5-15 parts of soybean meal, 8-15 parts of rice bran, 5-15 parts of beet sugar residues, 5-15 parts of vegetables, 1-3 parts of salt for livestock raising, 5-15 parts of soybean probiotic preparation, 1-3 parts of hawthorn, 1-3 parts of purslane, 0.5-1.5 parts of acanthopanax, 0.5-1.5 parts of dandelion and 0.5-1.5 parts of plantain.
The waste mushroom stick biological fermentation feed is prepared from the following raw materials in parts by weight: 30 parts of waste mushroom sticks, 10 parts of corn flour, 10 parts of soybean meal, 11 parts of rice bran, 10 parts of beet sugar residues, 10 parts of vegetables, 2 parts of salt for livestock raising, 10 parts of soybean probiotic preparation, 2 parts of hawthorn, 2 parts of purslane, 1 part of acanthopanax, 1 part of dandelion and 1 part of plantain.
The vegetable is selected from Chinese cabbage, caulis et folium Brassicae Capitatae, herba Apii Graveolentis or fructus Lycopersici Esculenti.
The soybean probiotic preparation is prepared by preparing soybean milk from soybeans and water and then adding probiotics for fermentation.
The weight ratio of the soybeans, the probiotics and the water is 1:0.6: 10.
The probiotics is prepared from the following raw materials: 10-20 parts of bifidobacterium, 10-20 parts of lactic acid bacteria, 15-25 parts of lactobacillus, 10-20 parts of bacillus subtilis, 10-30 parts of saccharomycetes and 10-20 parts of bacillus licheniformis.
The preparation method of the soybean probiotic preparation comprises the following steps:
(1) soaking semen glycines in water for 7-9 hr, cleaning, adding water, pulping into thick soybean pulp, and finely grinding into thick soybean pulp;
(2) sterilizing the soybean thick pulp after the fine grinding, cooling to 35-40 ℃, adding probiotics, and fermenting to obtain the soybean probiotic preparation.
In the step (1), the fine grinding is performed by four times of fine grinding processes of 60 meshes, 80 meshes, 100 meshes and 120 meshes respectively.
In the step (2), the sterilization is performed at the temperature of 110-120 ℃ for 10-20min, and the fermentation is performed at the temperature of 35-40 ℃ for 48-72 h.
The preparation method of the waste mushroom stick biological fermentation feed comprises the following steps:
(a) weighing the raw materials according to the weight of the raw materials for later use;
(b) cleaning vegetables, fructus crataegi, herba Portulacae, radix Acanthopanacis Senticosi, herba Taraxaci and herba plantaginis, adding water, mixing, and pulping;
(c) crushing the waste bacteria sticks, removing impurities, and sterilizing at 110-120 ℃ for 15-20min for later use;
(d) mixing the thick paste obtained in the step (b) with a soybean probiotic preparation, adding salt for livestock raising, stirring uniformly to dissolve the salt into a mixed solution, and uniformly mixing corn flour, soybean meal and rice bran to obtain mixed powder;
(e) uniformly mixing the crushed and sterilized waste mushroom sticks in the step (c) with the beet sugar residues to prepare mixed particles, uniformly mixing the mixed particles, the mixed powder and the mixed solution, and adding a proper amount of water in the mixing process to obtain a mixed material;
(f) and (3) putting the mixed material into a container, sealing, fermenting, drying until the water content is less than 15%, and granulating to obtain the waste mushroom-stick biological fermentation feed.
The water content of the mixed material in the step (e) is 28-32%.
The fermentation in the step (f) is carried out for 3 to 5 days at a temperature of between 35 and 40 ℃.
In order to facilitate understanding of the present invention, the raw materials and the drug effects of the present invention will be further described below.
The waste mushroom sticks are waste compost after edible mushrooms are cultivated, mainly comprise mycelium residues of the edible mushrooms and structural carbohydrates such as cellulose, hemicellulose, lignin and the like which are decomposed by the edible mushrooms, contain rich hyphal proteins, amino acids, crude fat, vitamins, mineral substances, mushroom bran polysaccharide, purine, organic acids, enzymes and bioactive substances, and have high nutritional value.
The corn flour contains rich nutrients, and contains a large amount of lecithin, linoleic acid, grain alcohol, vitamin E, cellulose and the like.
The soybean meal is one of 12 animal and plant oil meal feed products such as cottonseed meal, peanut meal, rapeseed meal and the like, which has the largest yield and the widest application. As a high protein, the soybean meal is a main raw material for preparing livestock and poultry feed, and can also be used as a raw material for preparing cake food, health food, cosmetics and antibiotics.
The rice bran is mainly prepared by processing pericarp, seed coat, endosperm, aleurone layer and embryo, and is a main byproduct in rice processing. The rice bran is rich in various nutrients and physiologically active substances, and contains 15% of protein, 16% to 22% of fat, 3% to 8% of sugar, 10% of water and about 125.1KJ/g of heat on average. The main fatty acids in the fat are unsaturated fatty acids such as oleic acid and linoleic acid, and contain high amount of vitamins, plant alcohol, dietary fiber, amino acids and minerals.
The sugar residue of the beet is a byproduct generated in sugar production of the beet, has higher nutritional value, contains 10 percent of crude protein, 20 percent of crude fiber, 60 percent of nitrogen-free extract and other nutritional ingredients, and has higher sugar content, better palatability, quick digestion and absorption and high nutritional value.
The hawthorn is Rosaceae, has sour and mild taste, contains rich nutrient substances, such as maslinic acid, citric acid, calcium, iron, phosphorus and other nutrient substances, and has the functions of promoting gastric secretion and increasing enzymes in the stomach.
The purslane contains rich dihydroxyethylamine, malic acid, glucose, calcium, phosphorus, iron, vitamin E, carotene, vitamin B, vitamin C and other nutrient substances. Purslane has a prominent feature in nutrition, and the omega-3 fatty acid content of the purslane is higher than that of human and plants. Omega-3 fatty acid can inhibit the absorption of human body to cholesterol, reduce the concentration of blood cholesterol, improve the elasticity of blood vessel wall, and is very beneficial to preventing and treating cardiovascular diseases.
Acanthopanax senticosus has the functions of invigorating middle-jiao and replenishing vital essence, strengthening muscles and bones and strengthening will, is light and durable for long-term use, and can be used together with other medicines to promote appetite, strengthen strength and avoid forgetting.
The herba Taraxaci is rich in vitamin A, vitamin C and potassium, and also contains ferrum, calcium, vitamin B2, vitamin B1, magnesium, vitamin B6, folic acid and copper. The specific element content is mainly water, and each 60 g of raw dandelion leaves contains 86% of water, 1.6 g of protein, 5.3 g of carbohydrate and 108.8 kilojoules of calories.
The herba plantaginis is whole plant of herba plantaginis and herba Plantago Plantaginaceae, and has sweet taste and cold property. Has effects of promoting urination, clearing heat away, improving eyesight, and eliminating phlegm. Can be used for treating urinary obstruction, stranguria with turbid urine, leukorrhagia, hematuria, jaundice, edema, dysentery, diarrhea, epistaxis, conjunctival congestion, sore throat, cough, skin ulcer, etc.
The bifidobacteria can inhibit the growth of harmful bacteria of a human body, resist the infection of pathogenic bacteria, synthesize vitamins required by the human body, promote the absorption of the human body to mineral substances, generate organic acids such as acetic acid, propionic acid, butyric acid, lactic acid and the like to stimulate the intestinal peristalsis, promote defecation, prevent constipation, inhibit the putrefaction of the intestinal tract, purify the intestinal environment, decompose carcinogenic substances, stimulate the immune system of the human body, and have important functions in the aspects of improving the disease resistance and the like.
Lactic acid bacteria are generic terms for a class of non-spore, gram-positive bacteria in which the main product of the fermentation sugars is lactic acid. The lactic acid bacteria can not only improve the nutritive value of the food, improve the flavor of the food and improve the preservation property and the added value of the food. Can regulate normal flora in gastrointestinal tract, maintain microecological balance, and improve food digestibility and biological value.
The lactobacillus is a group of microbes which live in the organism and are beneficial to the health of a host, can organize the invasion and the permanent planting of pathogenic bacteria to the intestinal tract, inhibit the pathogenic bacteria, resist infection, maintain the microecological balance of the intestinal tract, prevent and inhibit the occurrence of tumors, enhance the immunity of the organism, promote digestion, synthesize amino acids and vitamins, reduce cholesterol, inhibit the generation of endotoxin, delay aging, resist corrosion and the like.
Bacillus subtilis is a kind of Bacillus, an aerobic bacterium, and can decompose tryptophan to form indole by using protein, various sugars and starch. Can purify and repair soil, reduce crop diseases, promote the decomposition and utilization of crop straws and mature garbage, improve the quality of crop products, have irreplaceable effects on the aspects of food safety and the like.
The yeast is a unicellular fungus and can survive in an anoxic environment, and the yeast contains rich protein, fat, sugar, B vitamins and the like, as well as enzymes, coenzymes, ribonucleic acid, sterol and metabolic intermediates, so that the nutritive value of the fermented food can be improved.
Bacillus licheniformis, a gram-positive thermophilic bacterium common in soil. Such bacteria can also be found in the feathers of birds, particularly ground-dwelling birds (e.g., bromhidae) and aquatic birds (e.g., ducks), particularly in the feathers of the chest and back thereof. The optimum temperature for enzyme secretion was 37 ℃. It may exist in the form of spores, thus resisting the harsh environment; under good environment, the growth state can exist. The bacteria can regulate dysbacteriosis to achieve therapeutic purpose, and can promote organism to produce antibacterial active substance and kill pathogenic bacteria. Can produce active resisting matter, has unique biological oxygen-taking action mechanism and can inhibit the growth and propagation of pathogenic bacteria.
The bifidobacteria, the lactic acid bacteria, the lactobacilli, the bacillus subtilis, the saccharomycetes and the bacillus licheniformis in the invention are not specially limited, and all adopt commercially available raw materials. The invention utilizes living bacteria preparations such as bifidobacterium, lactobacillus and the like to carry out microorganism ecological anaerobic fermentation, has the functions of bacteriostasis, health care and immunity, and mass propagation of bacillus subtilis, can stimulate the production of interferon in the animal body, improve the concentration of immunoglobulin in the animal body, increase the activity of macrophage and enhance the immunity and disease resistance of the animal.
Through a large number of experiments, the inventor finds that the activity of probiotics can be improved when the probiotics and soybeans are firstly prepared into the soybean probiotic preparation and fermented into the feed, and the soybean thick paste can provide energy for the survival of the probiotics.
Compared with the prior art, the invention has the beneficial effects that:
(1) the waste bacteria stick biological fermentation feed is prepared by taking waste bacteria sticks as main raw materials and adding corn flour, soybean meal, rice bran, vegetables, sugar beet residues, a soybean probiotic preparation, hawthorn, purslane, acanthopanax, dandelion, plantain and the like, and is fermented by fully utilizing the rich components of mycelia, proteins, amino acids, vitamins, minerals and the like contained in the waste bacteria sticks and playing the medicinal effects of the hawthorn, the purslane, the acanthopanax, the dandelion and the plantain to increase the appetite of livestock and improve the disease resistance of the livestock, so that the fermented feed has the characteristics of rich nutrition, sour and fragrant taste, good palatability, rich mycoprotein, high absorption and conversion rate, high quality, no harm, low cost, improvement of the quality of cultured products and the like; the feed effectively utilizes agricultural wastes such as waste fungus sticks and the like, is beneficial to the development of circular agriculture, reduces the breeding cost, changes the feed structure, improves the quality of breeding products, and is a high-quality biological fermentation feed;
(2) the probiotics added in the feed and the soybeans are prepared into the soybean probiotic preparation, so that the number of the beneficial viable bacteria can be increased, the activity of the leavening agent can be improved, the microbial diversity in intestinal tracts can be obviously improved after animals eat the feed, the intestinal immune response is promoted, the microbial balance is realized, the prepared feed has more comprehensive nutritional ingredients, and the feed conversion rate is higher.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
In the following examples 1g is represented by 1 part by weight.
Example 1
The embodiment provides a waste mushroom-stick biological fermentation feed, which is prepared from the following raw materials: 20 parts of waste mushroom sticks, 15 parts of corn flour, 5 parts of soybean meal, 15 parts of rice bran, 5 parts of beet sugar residues, 15 parts of Chinese cabbages, 1 part of salt for livestock raising, 15 parts of soybean probiotic preparation, 1 part of hawthorn, 3 parts of purslane, 0.5 part of acanthopanax, 1.5 parts of dandelion and 0.5 part of plantain.
The soybean probiotic preparation is prepared by preparing soybean milk from soybeans and water, and then adding probiotics for fermentation, wherein the probiotics comprise the following raw materials: 10 parts of bifidobacterium, 20 parts of lactic acid bacteria, 15 parts of lactobacillus, 20 parts of bacillus subtilis and 10 parts of saccharomycetes, and 20 parts of bacillus licheniformis, wherein the specific preparation method comprises the following steps:
(1) soaking soybeans in water for 7h, cleaning to remove impurities, adding water, beating into thick soybean pulp, wherein the mass ratio of dry soybeans to water is 1:3 when the soybeans are soaked, and performing fine grinding treatment for four times of 60 meshes, 80 meshes, 100 meshes and 120 meshes;
(2) sterilizing the soybean thick pulp after fine grinding for 20min at 110 ℃, cooling the sterilized soybean thick pulp to 35 ℃, adding probiotics according to the proportion, fermenting for 72h at 35 ℃, sealing and keeping in the dark at constant temperature to obtain the soybean probiotic preparation.
The preparation method of the waste mushroom-stick biological fermentation feed comprises the following steps:
(a) weighing the raw materials according to the weight of the raw materials for later use;
(b) cleaning Chinese cabbage, hawthorn, purslane, acanthopanax, dandelion and plantain, adding water, mixing and beating into thick pulp, wherein the mass ratio of the total mass of the Chinese cabbage, the hawthorn, the purslane, the acanthopanax, the dandelion and the plantain to the added water is 1: 1;
(c) mixing the thick paste with a soybean probiotic preparation, adding salt for livestock raising, stirring uniformly to dissolve the salt into a mixed solution, and uniformly mixing corn flour, soybean meal and rice bran to obtain mixed powder;
(d) uniformly mixing the crushed and sterilized waste mushroom sticks in the step (c) with the beet sugar residues to prepare mixed particles, uniformly mixing the mixed particles, the mixed powder and the mixed solution, and adding a proper amount of water in the mixing process to ensure that the water content of the mixed material is 28 percent to obtain a mixed material;
(e) and (3) putting the mixed material into a container, compacting the material in the transfer process, sealing the container after the material is completely transferred, fermenting for 5 days at 35 ℃, drying until the moisture is less than 15%, and transferring into a granulator for granulation to obtain the waste mushroom stick biological fermentation feed.
Example 2
The waste mushroom stick biological fermentation feed comprises the following raw materials: 40 parts of waste mushroom sticks, 5 parts of corn flour, 15 parts of soybean meal, 8 parts of rice bran, 15 parts of beet sugar residues, 5 parts of cabbages, 3 parts of salt for livestock raising, 5 parts of soybean probiotic preparation, 3 parts of hawthorns, 1 part of purslane, 1.5 parts of acanthopanax, 0.5 part of dandelion and 1.5 parts of plantain.
The soybean probiotic preparation is prepared by preparing soybean milk from soybeans and water, and then adding probiotics for fermentation, wherein the probiotics comprise the following raw materials: 20 parts of bifidobacterium, 10 parts of lactic acid bacteria, 25 parts of lactobacillus, 10 parts of bacillus subtilis, 30 parts of saccharomycetes and 10 parts of bacillus licheniformis, and the specific preparation method comprises the following steps:
(1) soaking soybeans in water for 8h, cleaning to remove impurities, adding water, beating into thick soybean pulp, wherein the mass ratio of dry soybeans to water is 1:3 when the soybeans are soaked, and performing fine grinding treatment for four times of 60 meshes, 80 meshes, 100 meshes and 120 meshes;
(2) sterilizing the soybean thick pulp after fine grinding at 115 ℃ for 15min, cooling the sterilized soybean thick pulp to 37.5 ℃, adding probiotics according to the proportion, fermenting at 37.5 ℃ for 60h, sealing and keeping in the dark at constant temperature to obtain the soybean probiotic preparation.
The preparation method of the waste mushroom-stick biological fermentation feed comprises the following steps:
(a) weighing the raw materials according to the weight of the raw materials for later use;
(b) cleaning cabbage, hawthorn, purslane, acanthopanax, dandelion and plantain, adding water, mixing and beating into thick slurry, wherein the mass ratio of the total mass of the cabbage, the hawthorn, the purslane, the acanthopanax, the dandelion and the plantain to the added water is 1: 1.5;
(c) mixing the thick paste with a soybean probiotic preparation, adding salt for livestock raising, stirring uniformly to dissolve the salt into a mixed solution, and uniformly mixing corn flour, soybean meal and rice bran to obtain mixed powder;
(d) uniformly mixing the crushed and sterilized waste mushroom sticks in the step (c) with the beet sugar residues to prepare mixed particles, uniformly mixing the mixed particles, the mixed powder and the mixed solution, and adding a proper amount of water in the mixing process to ensure that the water content of the mixed material is 32 percent to obtain a mixed material;
(e) and (3) putting the mixed material into a container, compacting the material in the transfer process, sealing the container after the material is completely transferred, fermenting for 4 days at 37.5 ℃, drying until the moisture is less than 15%, and transferring into a granulator for granulation to obtain the waste mushroom stick biological fermentation feed.
Example 3
The waste mushroom stick biological fermentation feed comprises the following raw materials: 30 parts of waste mushroom sticks, 10 parts of corn flour, 10 parts of soybean meal, 11 parts of rice bran, 10 parts of beet sugar residues, 10 parts of celery, 2 parts of salt for livestock raising, 10 parts of soybean probiotic preparation, 2 parts of hawthorn, 2 parts of purslane, 1 part of acanthopanax, 1 part of dandelion and 1 part of plantain.
The soybean probiotic preparation is prepared by preparing soybean milk from soybeans and water, and then adding probiotics for fermentation, wherein the probiotics comprise the following raw materials: 15 parts of bifidobacterium, 15 parts of lactic acid bacteria, 20 parts of lactobacillus, 10-20 parts of bacillus subtilis, 10-30 parts of saccharomycetes and 15 parts of bacillus licheniformis, and the specific preparation method is as follows:
(1) soaking soybeans in water for 9h, cleaning to remove impurities, adding water, beating into thick soybean pulp, wherein the mass ratio of dry soybeans to water is 1:3 when the soybeans are soaked, and performing fine grinding treatment for four times of 60 meshes, 80 meshes, 100 meshes and 120 meshes;
(2) sterilizing the soybean thick pulp after fine grinding for 10min at 120 ℃, cooling the sterilized soybean thick pulp to 40 ℃, adding probiotics according to the proportion, fermenting for 48h at 40 ℃, sealing and keeping the soybean thick pulp in a dark place at constant temperature to obtain the soybean probiotic preparation.
The preparation method of the waste mushroom-stick biological fermentation feed comprises the following steps:
(a) weighing the raw materials according to the weight of the raw materials for later use;
(b) cleaning celery, hawthorn, purslane, acanthopanax, dandelion and plantain, adding water, mixing and beating into thick slurry, wherein the mass ratio of the total mass of the celery, the hawthorn, the purslane, the acanthopanax, the dandelion and the plantain to the added water is 1: 2;
(c) mixing the thick paste with a soybean probiotic preparation, adding salt for livestock raising, stirring uniformly to dissolve the salt into a mixed solution, and uniformly mixing corn flour, soybean meal and rice bran to obtain mixed powder;
(d) uniformly mixing the crushed and sterilized waste mushroom sticks in the step (c) with the beet sugar residues to prepare mixed particles, uniformly mixing the mixed particles, the mixed powder and the mixed solution, and adding a proper amount of water in the mixing process to ensure that the water content of the mixed material is 30 percent to obtain a mixed material;
(e) and (3) putting the mixed material into a container, compacting the material in the transfer process, sealing the container after the material is completely transferred, fermenting for 3 days at 40 ℃, drying until the moisture is less than 15%, and transferring into a granulator for granulation to obtain the waste mushroom stick biological fermentation feed.
Example 4
The waste mushroom stick biological fermentation feed comprises the following raw materials: 30 parts of waste mushroom sticks, 10 parts of corn flour, 10 parts of bean pulp, 11 parts of rice bran, 10 parts of beet sugar residues, 10 parts of tomatoes, 2 parts of salt for livestock raising, 10 parts of soybean probiotic preparation, 2 parts of hawthorn, 2 parts of purslane, 1 part of acanthopanax, 1 part of dandelion and 1 part of plantain.
The soybean probiotic preparation is prepared by preparing soybean milk from soybeans and water, and then adding probiotics for fermentation, wherein the probiotics comprise the following raw materials: 15 parts of bifidobacterium, 15 parts of lactic acid bacteria, 20 parts of lactobacillus, 10-20 parts of bacillus subtilis, 12 parts of saccharomycetes and 18 parts of bacillus licheniformis, and the preparation method comprises the following steps:
(1) soaking soybeans in water for 9h, cleaning to remove impurities, adding water, beating into thick soybean pulp, wherein the mass ratio of dry soybeans to water is 1:3 when the soybeans are soaked, and performing fine grinding treatment for four times of 60 meshes, 80 meshes, 100 meshes and 120 meshes;
(2) sterilizing the soybean thick pulp after fine grinding for 10min at 120 ℃, cooling the sterilized soybean thick pulp to 40 ℃, adding probiotics according to the proportion, fermenting for 48h at 40 ℃, sealing and keeping the soybean thick pulp in a dark place at constant temperature to obtain the soybean probiotic preparation.
The preparation method of the waste mushroom-stick biological fermentation feed comprises the following steps:
(a) weighing the raw materials according to the weight of the raw materials for later use;
(b) cleaning tomato, hawthorn, purslane, acanthopanax, dandelion and plantain, adding water, mixing and beating into thick pulp, wherein the mass ratio of the total mass of the tomato, the hawthorn, the purslane, the acanthopanax, the dandelion and the plantain to the added water is 1: 2;
(c) mixing the thick paste with a soybean probiotic preparation, adding salt for livestock raising, stirring uniformly to dissolve the salt into a mixed solution, and uniformly mixing corn flour, soybean meal and rice bran to obtain mixed powder;
(d) uniformly mixing the crushed and sterilized waste mushroom sticks in the step (c) with the beet sugar residues to prepare mixed particles, uniformly mixing the mixed particles, the mixed powder and the mixed solution, and adding a proper amount of water in the mixing process to ensure that the water content of the mixed material is 30 percent to obtain a mixed material;
(e) and (3) putting the mixed material into a container, compacting the material in the transfer process, sealing the container after the material is completely transferred, fermenting for 3 days at 40 ℃, drying until the moisture is less than 15%, and transferring into a granulator for granulation to obtain the waste mushroom stick biological fermentation feed.
Comparative example 1
The biological fermentation feed provided by the comparative example does not add waste gas bacteria sticks, and other raw materials and preparation methods are the same as those of example 3.
Comparative example 2
The biological fermentation feed provided by the comparative example does not add traditional Chinese medicine components of hawthorn, purslane, acanthopanax, dandelion and plantain, and other raw materials and preparation methods are the same as those in example 3.
Test example 1
Selecting 400 test beef cattle, randomly dividing the beef cattle into 4 groups, wherein each group comprises 100 beef cattle, feeding the fermented feed of the comparative example 1 to the first group, and marking as a control group 1; feeding the fermented feed of the comparative example 2 to the second group, and marking as a control group 2; the third group was fed with commercial cattle feed (produced by Zhucheng strongly lin feeds Co., Ltd.) and designated as control group 3; the fourth group was fed with the fermented feed of example 3 of the present invention and recorded as test group; the same feed was fed to each group under the same conditions for a test period of 4 months, and the average daily gain was counted after the end of the test, with the results shown in table 1.
TABLE 1
As can be seen from Table 1, the average daily gain of beef cattle fed by the waste bacteria stick biological fermentation feed is obviously higher than that of beef cattle fed by the comparative example 1, the comparative example 2 and commercial feed, namely, the waste bacteria stick biological fermentation feed has better palatability and is easier to digest and absorb, and the traditional Chinese medicine components contained in the feed are beneficial to increasing the appetite of livestock, improving the disease resistance of the livestock, reducing the occurrence of diseases and accelerating the growth speed of the cattle.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. The waste mushroom stick biological fermentation feed is characterized by being prepared from the following raw materials in parts by weight: 20-40 parts of waste mushroom sticks, 5-15 parts of corn flour, 5-15 parts of soybean meal, 8-15 parts of rice bran, 5-15 parts of beet sugar residues, 5-15 parts of vegetables, 1-3 parts of salt for livestock raising, 5-15 parts of soybean probiotic preparation, 1-3 parts of hawthorn, 1-3 parts of purslane, 0.5-1.5 parts of acanthopanax, 0.5-1.5 parts of dandelion and 0.5-1.5 parts of plantain.
2. The waste mushroom-stick biological fermentation feed as claimed in claim 1, which is prepared from the following raw materials in parts by weight: 30 parts of waste mushroom sticks, 10 parts of corn flour, 10 parts of soybean meal, 11 parts of rice bran, 10 parts of beet sugar residues, 10 parts of vegetables, 2 parts of salt for livestock raising, 10 parts of soybean probiotic preparation, 2 parts of hawthorn, 2 parts of purslane, 1 part of acanthopanax, 1 part of dandelion and 1 part of plantain.
3. The waste mushroom-stick biofermented feed according to claim 1, wherein the vegetable is Chinese cabbage, celery or tomato.
4. The waste mushroom-stick biofermented feed according to claim 1, wherein the probiotic preparation of soybean is prepared by preparing soybean milk from soybean and water, and then adding probiotic to ferment;
the weight ratio of the soybeans, the probiotics and the water is 1:0.6: 10.
5. The waste mushroom-stick biological fermentation feed as claimed in claim 4, wherein the probiotics comprise the following raw materials in parts by weight: 10-20 parts of bifidobacterium, 10-20 parts of lactic acid bacteria, 15-25 parts of lactobacillus, 10-20 parts of bacillus subtilis, 10-30 parts of saccharomycetes and 10-20 parts of bacillus licheniformis.
6. The waste mushroom-stick biofermented feed according to claim 4, wherein the preparation method of the probiotic preparation of soybean comprises the following steps:
(1) soaking semen glycines in water for 7-9 hr, cleaning, adding water, pulping into thick soybean pulp, and finely grinding into thick soybean pulp;
(2) sterilizing the soybean thick pulp after the fine grinding, cooling to 35-40 ℃, adding probiotics, and fermenting to obtain the soybean probiotic preparation.
7. The waste mushroom-stick biofermented feed according to claim 6, wherein in the step (1), the fine refining is performed by four times of fine refining processes of 60 mesh, 80 mesh, 100 mesh and 120 mesh, respectively.
8. The waste mushroom-stick biofermentation feed as claimed in claim 6, wherein in the step (2), the sterilization is performed at 110-120 ℃ for 10-20min, and the fermentation is performed at 35-40 ℃ for 48-72 h.
9. A method for preparing a waste mushroom-stick biofermented feed according to any one of claims 1 to 8, comprising the steps of:
(a) weighing the raw materials according to the weight of the raw materials for later use;
(b) cleaning vegetables, fructus crataegi, herba Portulacae, radix Acanthopanacis Senticosi, herba Taraxaci and herba plantaginis, adding water, mixing, and pulping;
(c) crushing the waste bacteria sticks, removing impurities, and sterilizing at 110-120 ℃ for 15-20min for later use;
(d) mixing the thick paste obtained in the step (b) with a soybean probiotic preparation, adding salt for livestock raising, stirring uniformly to dissolve the salt into a mixed solution, and uniformly mixing corn flour, soybean meal and rice bran to obtain mixed powder;
(e) uniformly mixing the crushed and sterilized waste mushroom sticks in the step (c) with the beet sugar residues to prepare mixed particles, uniformly mixing the mixed particles, the mixed powder and the mixed solution, and adding a proper amount of water in the mixing process to obtain a mixed material;
(f) and (3) putting the mixed material into a container, sealing, fermenting, drying until the water content is less than 15%, and granulating to obtain the waste mushroom-stick biological fermentation feed.
10. The method for preparing the biological fermented feed of the waste mushroom sticks according to claim 8, wherein the water content of the mixed material in the step (e) is 28-32%;
the fermentation in the step (f) is carried out for 3 to 5 days at a temperature of between 35 and 40 ℃.
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