CN107007640A - A kind of production method for preventing and treating livestock and birds respiratory disease product - Google Patents
A kind of production method for preventing and treating livestock and birds respiratory disease product Download PDFInfo
- Publication number
- CN107007640A CN107007640A CN201710427501.1A CN201710427501A CN107007640A CN 107007640 A CN107007640 A CN 107007640A CN 201710427501 A CN201710427501 A CN 201710427501A CN 107007640 A CN107007640 A CN 107007640A
- Authority
- CN
- China
- Prior art keywords
- chinese herbal
- production method
- herbal medicine
- respiratory disease
- preventing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7016—Disaccharides, e.g. lactose, lactulose
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/716—Glucans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/064—Saccharomycetales, e.g. baker's yeast
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/007—Pulmonary tract; Aromatherapy
- A61K9/0073—Sprays or powders for inhalation; Aerolised or nebulised preparations generated by other means than thermal energy
- A61K9/0078—Sprays or powders for inhalation; Aerolised or nebulised preparations generated by other means than thermal energy for inhalation via a nebulizer such as a jet nebulizer, ultrasonic nebulizer, e.g. in the form of aqueous drug solutions or dispersions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Nutrition Science (AREA)
- Zoology (AREA)
- Physiology (AREA)
- Dispersion Chemistry (AREA)
- Otolaryngology (AREA)
- Pulmonology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of production method for preventing and treating livestock and birds respiratory disease product, the probiotics such as Bifidobacterium Bifidum, lactic acid bacteria, Bacillus, saccharomycete and its metabolite and Chinese herbal medicine tunning, prebiotics, respiratory tract and the one or more of of intestinal mucosa reparative factor are mixed by a certain percentage.Through the above way, what the present invention was provided prevents and treats the production method of livestock and birds respiratory disease product, the product coordinates oral administration effectively to prevent and treat livestock and birds respiratory disease by atomized medicine introducing, reduce or prevent the use of antibiotic in breeding process, suppress the generation of breathing problem by improving the health of respiratory mucosa and intestinal mucosa, improve feed digestibility, reduce aquaculture cost, the residual of antibiotic in animal product is reduced, the animal product for production green, noresidue provides technology and product support.
Description
Technical field
It is specifically a kind of the present invention relates to a kind of product processes for preventing and treating livestock and birds respiratory disease
Mixed by a certain percentage by the one or more of probiotics and its metabolite, Chinese herbal medicine, respiratory mucosa reparative factor.
Background technology
The aquaculture of China passes through the development of decades, scale, intensive degree more and more higher, with New Oversea Products kind
Introduction, circulation widens, and the increase of stocking density is that the prevalence of breathing problem creates objective condition, respiratory tract
Disease also increasingly becomes a big obstacle of aquaculture development.
1 )Feeding management be not good at or stress factor caused by respiratory diseases(Environmental factor)
a:Cold stress, such as weather cool, and thermal source breaks down, and the factor such as excessive of divulging information and interior have harmful wind or draught to cause house
Warm fusion, causes the respiratory symptoms such as sneeze, cough;
b:Dust and pernicious gas in improper ventilation, house(Ammonia, hydrogen sulfide, carbon dioxide etc.)Too high levels, if now divulging information
Bad, then harmful gas concentration is too high, stimulates respiratory mucosa, causes respiratory symptom;
c:Density is too high in pouity dwelling place
d:In band chicken spraying disinfection, the big disinfectant of excitant is used:Such as chloride class, Lysol, can stimulate respiratory tract to stick
Film, causes respiratory symptom;
Above stress factor, can all stimulate respiratory mucosa, not only cause corresponding respiratory symptom, while also making respiratory tract
The resistance of mucous membrane is reduced, now some pathogenic microorganisms(Such as bacterium, mycoplasma)It will avail oneself of the opportunity to get in, cause more serious biography
Metachromia breathing problem, now, it is invalid to throw the medicines such as antibiotic in the treatment time, it is necessary to which coordinating the improvement of environment could obtain
Good effect.
2)Respiratory diseases caused by vaccine improper use
3)Strong poison seedling repeatedly, Use out of range when, internal pathogen may be caused to accumulate, cause vaccine virus to infect
4)By pathogenic microorganism(Virus, bacterium and mycoplasma etc.)Caused respiratory diseases
Ewcastle disease, bird flu, infective bronchitis, infectious laryngotracheitis, chronic respiratory tract disease, infective rhinitis, large intestine
Bacillosis etc. has respiratory symptom performance, but their cause of disease is different.And more complicated is two kinds or two kinds
Above respiratory pathogen acts on animal respiratory simultaneously or successively, pathogenic synergy can be produced between them, than single
Pathogen associated diseases are much serious.Especially some inhibitive ability of immunity diseases, occur the chicken group of breathing problem and exist to exempt from
When epidemic disease suppresses pathogen, breathing problem can be very serious and uncontrollable.
The content of the invention
The present invention solves the technical problem of provide a kind of life for preventing and treating livestock and birds respiratory disease product
Production method, the product coordinates oral administration effectively to prevent and treat livestock and birds respiratory disease by atomized medicine introducing, reduce or
Prevent the use of antibiotic in breeding process, suppress the hair of breathing problem by improving the health of respiratory mucosa and intestinal mucosa
Life, improves feed digestibility, reduces aquaculture cost, reduces the residual of antibiotic in animal product, green, noresidue for production
Animal product provides technology and product support.
In order to solve the above technical problems, one aspect of the present invention is:It can be prevented and treated there is provided one kind
The production method of livestock and birds respiratory disease product, by probiotics and its metabolite and Chinese herbal medicine tunning, prebiotics, breathing
Road is mixed by a certain percentage with the one or more of intestinal mucosa reparative factor, and described preparation method comprises the following steps:
1)Prepare seed liquor
By the one or more in Bifidobacterium Bifidum, lactic acid bacteria, Bacillus, saccharomycete, the liquid seeds training after the corresponding sterilizing of access
Support in base, cultivating 24-72h in 24-38 DEG C obtains seed liquor;
2)Prepare probiotics fermention liquid
By the one or more in Bifidobacterium Bifidum, lactic acid bacteria, Bacillus, saccharomycete, the liquid fermentation training after the corresponding sterilizing of access
Support in base, cultivating 48-96h in 24-38 DEG C obtains zymotic fluid;
3)Prepare probiotics and its metabolite
Corresponding bacterium mud is obtained after probiotics fermention liquid is centrifuged, carrier is then added and is freeze-dried, it is ensured that be living after drying
The quantity of bacterium is in certain valid value range, and fermented supernatant fluid after centrifugation carries out being lotion after cryogenic vacuum concentration, by with
Upper method respectively obtains probiotics and dries viable bacteria powder and supernatant cream mud;
Or, it is that probiotics fermention liquid is directly carried out to cryogenic vacuum to concentrate to be cream mud;
4)Prepare Chinese herbal medicine tunning
By Chinese herbal medicine after ultramicro grinding is handled, the fermentation rich in Chinese herbal medicine effective ingredientses is obtained after probiotics fermention and is produced
Thing, is then prepared into Chinese recipe mud;
5)Preparation can carry out the preparation of atomized medicine introducing
Probiotics and its metabolite are pressed one with the one or more in Chinese herbal medicine tunning, respiratory mucosa reparative factor
Certainty ratio, which is mixed with, can carry out the preparation of atomized medicine introducing;
6)Prepare orally available preparation
By probiotics and its metabolite and Chinese herbal medicine tunning, respiratory mucosa reparative factor, intestinal mucosa reparative factor
In one or more be mixed with orally available preparation by a certain percentage;
7)Compatibility is combined with quantitative prebiotics.
In a preferred embodiment of the present invention, described probiotics fermention liquid is through 3000-6000r/min × 5-20min
Centrifugal treating.
In a preferred embodiment of the present invention, described Chinese herbal medicine uses one effective to treatment breathing problem to hit
Herbal medicine, includes but is not limited to:Radix Isatidis, gypsum, the dried rhizome of rehmannia, balloonflower root, the coptis, radix scutellariae, cape jasmine, radix scrophulariae, the capsule of weeping forsythia, two flowers, safflower,
Rheum officinale, saltcake, radix glycyrrhizae, conjunction cape jasmine, honeysuckle, myrobalan, caulis bambusae in taenian, golden cypress, the root of herbaceous peony, gypsum, saltcake, folium isatidis, Radix Et Rhizoma Rhei, Soviet Union
It is son, gingko, almond, double skins, tussilago, Chinese ephedra, cordate houttuynia, ginseng, ganoderma lucidum, honeysuckle, schizonepeta, windproof, cassia twig, loguat leaf, old
Tealeaves, Plant Soot, the wind-weed, fritillaria, balloonflower root, mulberry skin, loguat leaf, lepidium seed, the tuber of dwarf lilyturf, alstonia-leaf, Rajmahal creeper, cacumen biotae, stone green pepper
Grass, pappy shell, Verbena officinalis, kuh-seng, the tuber of pinellia, cape jasmine, Radix Angelicae Sinensis, the achene of Siberian cocklebur, the bighead atractylodes rhizome, injustice silk, radix bupleuri, fructus psoaleae, dried orange peel.
In a preferred embodiment of the present invention, fineness after described Chinese herbal medicine ultra-fine powder is broken 100-6000 mesh it
Between.
In a preferred embodiment of the present invention, described Chinese herbal medicine probiotics fermention mode is divided into solid fermentation and liquid
Fermentation.
In a preferred embodiment of the present invention, the mode of described solid fermentation is divided into aerobic fermentation, anaerobic fermentation, elder generation
It is aerobic after aerobic rear anaerobism or first anaerobism.
In a preferred embodiment of the present invention, described liquid fermentation is quantitative bacterium will to be added after Chinese herbal medicine micro mist
Culture medium accesses corresponding probiotics strain after high-temperature sterilization and carries out aerobic or anaerobic fermentation.
In a preferred embodiment of the present invention, described respiratory mucosa reparative factor can answer respiratory tract using all
Material, including but not limited to EGF.
In a preferred embodiment of the present invention, described intestinal mucosa reparative factor can repair the thing of enteron aisle using all
Matter, including but not limited to EGF.
In a preferred embodiment of the present invention, described prebiotics be oligosaccharide, polysaccharide, vegetable Chinese herbal medicine extract,
Protein hydrolysate or polyalcohol.
The beneficial effects of the invention are as follows:The production method for preventing and treating livestock and birds respiratory disease product of the present invention,
The product coordinates oral administration effectively to prevent and treat livestock and birds respiratory disease by atomized medicine introducing, reduces or prevents cultivation
During antibiotic use, suppress the generation of breathing problem by improving the health of respiratory mucosa and intestinal mucosa, improve
Feed digestibility, reduces aquaculture cost, reduces the residual of antibiotic in animal product, for production green, the animal system of noresidue
Product provide technology and product support.
Brief description of the drawings
Technical scheme in order to illustrate the embodiments of the present invention more clearly, makes required in being described below to embodiment
Accompanying drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the present invention, for
For those of ordinary skill in the art, on the premise of not paying creative work, it can also obtain other according to these accompanying drawings
Accompanying drawing, wherein:
Fig. 1 is the structure stream of a preferred embodiment of the production method that the present invention can prevent and treat livestock and birds respiratory disease product
Cheng Tu.
Embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, this area is common
All other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model that the present invention is protected
Enclose.
As shown in figure 1, the embodiment of the present invention includes:
A kind of production method for preventing and treating livestock and birds respiratory disease product, by Bifidobacterium Bifidum, lactic acid bacteria, Bacillus, ferment
The probiotics such as female bacterium and its metabolite and the one of Chinese herbal medicine tunning, prebiotics, respiratory tract and intestinal mucosa reparative factor
Kind or it is several mix by a certain percentage, described preparation method comprises the following steps:
1)Prepare seed liquor
By the one or more in Bifidobacterium Bifidum, lactic acid bacteria, Bacillus, saccharomycete, the liquid seeds training after the corresponding sterilizing of access
Support in base, cultivating 24-72h in 24-38 DEG C obtains seed liquor;
2)Prepare probiotics fermention liquid
By the one or more in Bifidobacterium Bifidum, lactic acid bacteria, Bacillus, saccharomycete, the liquid fermentation training after the corresponding sterilizing of access
Support in base, cultivating 48-96h in 24-38 DEG C obtains zymotic fluid;
3)Prepare probiotics and its metabolite
Corresponding bacterium mud is obtained after probiotics fermention liquid is centrifuged, carrier is then added and is freeze-dried, it is ensured that be living after drying
The quantity of bacterium is in certain valid value range, and fermented supernatant fluid after centrifugation carries out being lotion after cryogenic vacuum concentration, by with
Upper method respectively obtains probiotics and dries viable bacteria powder and supernatant cream mud, wherein, described probiotics fermention liquid is through 3000-
6000r/min × 5-20min centrifugal treatings;
Or, it is that probiotics fermention liquid is directly carried out to cryogenic vacuum to concentrate to be cream mud;
4)Prepare Chinese herbal medicine tunning
By Chinese herbal medicine after ultramicro grinding is handled, the fermentation rich in Chinese herbal medicine effective ingredientses is obtained after probiotics fermention and is produced
Thing, is then prepared into Chinese recipe mud, wherein, fineness after described Chinese herbal medicine ultra-fine powder is broken 100-6000 mesh it
Between;
5)Preparation can carry out the preparation of atomized medicine introducing
Probiotics and its metabolite are pressed one with the one or more in Chinese herbal medicine tunning, respiratory mucosa reparative factor
Certainty ratio, which is mixed with, can carry out the preparation of atomized medicine introducing;
6)Prepare orally available preparation
By probiotics and its metabolite and Chinese herbal medicine tunning, respiratory mucosa reparative factor, intestinal mucosa reparative factor
In one or more be mixed with orally available preparation by a certain percentage;
7)Compatibility is combined with quantitative prebiotics.
In above-mentioned, described probiotics strain is as defined in Minister Agriculture of China in microorganism fodder strain Directory Scope.
Described Chinese herbal medicine be Radix Isatidis, gypsum, the dried rhizome of rehmannia, balloonflower root, the coptis, radix scutellariae, cape jasmine, radix scrophulariae, the capsule of weeping forsythia, two flower,
Safflower, rheum officinale, saltcake, radix glycyrrhizae, conjunction cape jasmine, honeysuckle, myrobalan, caulis bambusae in taenian, golden cypress, the root of herbaceous peony, gypsum, saltcake, folium isatidis, river are big
Huang, perillaseed, gingko, almond, double skins, tussilago, Chinese ephedra, cordate houttuynia, ginseng, ganoderma lucidum, honeysuckle, schizonepeta, windproof, cassia twig, loquat
Leaf, old tea, Plant Soot, the wind-weed, fritillaria, balloonflower root, mulberry skin, loguat leaf, lepidium seed, the tuber of dwarf lilyturf, alstonia-leaf, Rajmahal creeper, cacumen biotae,
Boenninghausenia sessilicarpa, pappy shell, Verbena officinalis, kuh-seng, the tuber of pinellia, cape jasmine, Radix Angelicae Sinensis, the achene of Siberian cocklebur, the bighead atractylodes rhizome, injustice silk, radix bupleuri, fructus psoaleae, dried orange peel
Deng to treatment breathing problem effectively all Chinese herbal medicines.
Further, described Chinese herbal medicine probiotics fermention mode is divided into solid fermentation and liquid fermentation, and wherein solid is sent out
Ferment can be divided into the several ways such as aerobic after aerobic fermentation, anaerobic fermentation, first aerobic rear anaerobism, first anaerobism.Liquid fermentation is by
Quantitative bacteria culture media is added after herbal medicine micro mist corresponding probiotics strain is accessed after high-temperature sterilization and carried out aerobic or detest
Aerobe fermentation.
Wherein, described respiratory mucosa reparative factor is EGF(People's oligopeptides -1)The thing of respiratory tract can be repaired Deng all
Matter;Described intestinal mucosa reparative factor is EGF(People's oligopeptides -1)The material of enteron aisle can be repaired Deng all.
Further, described prebiotics be oligosaccharide, it is polysaccharide, vegetable Chinese herbal medicine extract, protein hydrolysate, many
First alcohol etc..
Embodiment:
Probiotics by taking lactobacillus acidophilus, bacillus subtilis, saccharomyces cerevisiae as an example, Chinese herbal medicine by taking Radix Isatidis as an example, prebiotics with
Exemplified by oligoisomaltose.
1)Prepare probiotics seed liquor
By lactobacillus acidophilus, bacillus subtilis test tube strains, in the liquid seed culture medium after the corresponding sterilizing of access, in 37 DEG C
Culture 36h obtains seed liquor;
By saccharomyces cerevisiae test tube strains, in the liquid seed culture medium after the corresponding sterilizing of access, cultivate 48h in 28 DEG C and obtain seed
Liquid.
2)Prepare zymotic fluid
By lactobacillus acidophilus seed liquor, in the liquid fermentation medium after the corresponding sterilizing of access, cultivate 72h in 37 DEG C and fermented
Liquid;
By bacillus subtilis seed liquor, in the liquid fermentation medium after the corresponding sterilizing of access, cultivate 48h in 37 DEG C and sent out
Zymotic fluid;
By saccharomyces cerevisiae seed liquor, in the liquid fermentation medium after the corresponding sterilizing of access, cultivate 48h in 28 DEG C and fermented
Liquid.
3)Prepare probiotics and its metabolite
By lactobacillus acidophilus, bacillus subtilis fermentation liquor 6000r/min × 10min centrifugal treatings, collect and add respectively after bacterium mud
Enter and be freeze-dried in the skimmed milk solution that concentration is 20%;
By fermentation by saccharomyces cerevisiae liquid 3000r/min × 5min centrifugal treatings, collect after bacterium mud added by 20% addition it is dry
In soluble starch, 37 DEG C of vacuum and low temperature drying;
Lactobacillus acidophilus, bacillus subtilis, saccharomyces cerevisiae centrifugation after fermentation supernatant are added into drying by 80% addition
In soluble starch, 37 DEG C of vacuum and low temperature drying.
4)Prepare Chinese herbal medicine tunning
By Radix Isatidis after ultramicro grinding to 380 mesh, by 1:0.5 amount adds the bacterium solution containing profitable probliotics, after stirring
37 DEG C fermentation 10 days after take out 80 DEG C dry to obtain Radix Isatidis solid fermentation thing powder;
By Radix Isatidis after ultramicro grinding to 380 mesh, added by 10% amount and contain probiotics fermention culture medium bacterium solution, through 121
DEG C × 30min sterilization treatments after, after zymotic fluid 6000r/min × 10min centrifugal treatings supernatant will be taken to be concentrated through cryogenic vacuum
To the weak solution that dry matter content is 5.0%;
By Radix Isatidis after ultramicro grinding to 380 mesh, added by 10% amount and contain probiotics fermention culture medium bacterium solution, through 121
DEG C × 30min sterilization treatments after, by the drying of 80 DEG C of zymotic fluid solid residue to moisture below 14%.
5)Prebiotics uses food-grade oligoisomaltose.
Product formula is constituted
Spray delivery is formulated:100 grams of the powder of fermented liquid supernatant containing lactobacillus acidophilus, fermentation of bacillus subtilis in per 1000ml products
100 grams of liquid supernatant powder, 200 grams of fermentation by saccharomyces cerevisiae liquid supernatant powder, Radix Isatidis tunning content are 5% weak solution 500ml, low
150 grams of IMO, adds distilled water and is settled to 1000ml.
Formulation for oral delivery:50 grams of powder containing lactobacillus acidophilus, 80 grams of bacillus subtilis powder, wine brewing in every 1000 grams of products
20 grams of yeast bacterium powder, 300 grams of oligoisomaltose.On 200 grams of bacillus subtilis fermentation liquor supernatant powder, fermentation by saccharomyces cerevisiae liquid
Clear 100 grams of powder, 50 grams of Radix Isatidis zymotic fluid solid residue, 200 grams of Radix Isatidis solid fermentation thing powder.
In summary, the production method for preventing and treating livestock and birds respiratory disease product of the invention, the product passes through
Atomized medicine introducing coordinates oral administration effectively to prevent and treat livestock and birds respiratory disease, reduces or prevents antibiosis in breeding process
The use of element, suppresses the generation of breathing problem by improving the health of respiratory mucosa and intestinal mucosa, improves feed digestibility,
Reduce aquaculture cost, reduce animal product in antibiotic residual, for production green, noresidue animal product provide technology and
Product support.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair
Equivalent structure or equivalent flow conversion that bright description is made, or directly or indirectly it is used in other related technology necks
Domain, is included within the scope of the present invention.
Claims (10)
1. a kind of production method for preventing and treating livestock and birds respiratory disease product, it is characterised in that by probiotics and its generation
Thank product and Chinese herbal medicine tunning, prebiotics, respiratory tract and intestinal mucosa reparative factor it is one or more of by a certain percentage
Mixing, described preparation method comprises the following steps:
1)Prepare seed liquor
By the one or more in Bifidobacterium Bifidum, lactic acid bacteria, Bacillus, saccharomycete, the liquid seeds training after the corresponding sterilizing of access
Support in base, cultivating 24-72h in 24-38 DEG C obtains seed liquor;
2)Prepare probiotics fermention liquid
By the one or more in Bifidobacterium Bifidum, lactic acid bacteria, Bacillus, saccharomycete, the liquid fermentation training after the corresponding sterilizing of access
Support in base, cultivating 48-96h in 24-38 DEG C obtains zymotic fluid;
3)Prepare probiotics and its metabolite
Corresponding bacterium mud is obtained after probiotics fermention liquid is centrifuged, carrier is then added and is freeze-dried, it is ensured that be living after drying
The quantity of bacterium is in certain valid value range, and fermented supernatant fluid after centrifugation carries out being lotion after cryogenic vacuum concentration, by with
Upper method respectively obtains probiotics and dries viable bacteria powder and supernatant cream mud;
Or, it is that probiotics fermention liquid is directly carried out to cryogenic vacuum to concentrate to be cream mud;
4)Prepare Chinese herbal medicine tunning
By Chinese herbal medicine after ultramicro grinding is handled, the fermentation rich in Chinese herbal medicine effective ingredientses is obtained after probiotics fermention and is produced
Thing, is then prepared into Chinese recipe mud;
5)Preparation can carry out the preparation of atomized medicine introducing
Probiotics and its metabolite are pressed one with the one or more in Chinese herbal medicine tunning, respiratory mucosa reparative factor
Certainty ratio, which is mixed with, can carry out the preparation of atomized medicine introducing;
6)Prepare orally available preparation
By probiotics and its metabolite and Chinese herbal medicine tunning, respiratory mucosa reparative factor, intestinal mucosa reparative factor
In one or more be mixed with orally available preparation by a certain percentage;
7)Compatibility is combined with quantitative prebiotics.
2. the production method according to claim 1 for preventing and treating livestock and birds respiratory disease product, it is characterised in that
Described probiotics fermention liquid is through 3000-6000r/min × 5-20min centrifugal treatings.
3. the production method according to claim 1 for preventing and treating livestock and birds respiratory disease product, it is characterised in that
Described Chinese herbal medicine uses all Chinese herbal medicines effective to treatment breathing problem, includes but is not limited to:Radix Isatidis, gypsum,
The dried rhizome of rehmannia, balloonflower root, the coptis, radix scutellariae, cape jasmine, radix scrophulariae, the capsule of weeping forsythia, two flowers, safflower, rheum officinale, saltcake, radix glycyrrhizae, close cape jasmine, honeysuckle, scold
Son, caulis bambusae in taenian, golden cypress, the root of herbaceous peony, gypsum, saltcake, folium isatidis, Radix Et Rhizoma Rhei, perillaseed, gingko, almond, double skins, tussilago, Chinese ephedra,
Cordate houttuynia, ginseng, ganoderma lucidum, honeysuckle, schizonepeta, windproof, cassia twig, loguat leaf, old tea, Plant Soot, the wind-weed, fritillaria, balloonflower root, mulberry
Skin, loguat leaf, lepidium seed, the tuber of dwarf lilyturf, alstonia-leaf, Rajmahal creeper, cacumen biotae, boenninghausenia sessilicarpa, pappy shell, Verbena officinalis, kuh-seng, the tuber of pinellia, Cape jasmine
Son, Radix Angelicae Sinensis, the achene of Siberian cocklebur, the bighead atractylodes rhizome, injustice silk, radix bupleuri, fructus psoaleae, dried orange peel.
4. the production method according to claim 1 for preventing and treating livestock and birds respiratory disease product, it is characterised in that
Fineness after described Chinese herbal medicine ultra-fine powder is broken is between 100-6000 mesh.
5. the production method according to claim 1 for preventing and treating livestock and birds respiratory disease product, it is characterised in that
Described Chinese herbal medicine probiotics fermention mode is divided into solid fermentation and liquid fermentation.
6. the production method according to claim 5 for preventing and treating livestock and birds respiratory disease product, it is characterised in that
The mode of described solid fermentation is divided into aerobic after aerobic fermentation, first anaerobic fermentation, aerobic rear anaerobism or first anaerobism.
7. the production method according to claim 5 for preventing and treating livestock and birds respiratory disease product, it is characterised in that
Described liquid fermentation is will to add quantitative bacteria culture media after Chinese herbal medicine micro mist to access corresponding benefit after high-temperature sterilization
Raw bacterium strain carries out aerobic or anaerobic fermentation.
8. the production method according to claim 1 for preventing and treating livestock and birds respiratory disease product, it is characterised in that
Described respiratory mucosa reparative factor can answer the material of respiratory tract, including but not limited to EGF using all.
9. the production method according to claim 1 for preventing and treating livestock and birds respiratory disease product, it is characterised in that
Described intestinal mucosa reparative factor can repair the material of enteron aisle, including but not limited to EGF using all.
10. the production method according to claim 1 for preventing and treating livestock and birds respiratory disease product, its feature exists
In described prebiotics is oligosaccharide, polysaccharide, vegetable Chinese herbal medicine extract, protein hydrolysate or polyalcohol.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710427501.1A CN107007640A (en) | 2017-06-08 | 2017-06-08 | A kind of production method for preventing and treating livestock and birds respiratory disease product |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710427501.1A CN107007640A (en) | 2017-06-08 | 2017-06-08 | A kind of production method for preventing and treating livestock and birds respiratory disease product |
Publications (1)
Publication Number | Publication Date |
---|---|
CN107007640A true CN107007640A (en) | 2017-08-04 |
Family
ID=59451107
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710427501.1A Pending CN107007640A (en) | 2017-06-08 | 2017-06-08 | A kind of production method for preventing and treating livestock and birds respiratory disease product |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107007640A (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109043159A (en) * | 2018-08-24 | 2018-12-21 | 广东群林牧业有限公司 | A kind of internal expelling parasite ewe feed formulation method |
CN111034855A (en) * | 2019-12-31 | 2020-04-21 | 山西大禹生物工程股份有限公司 | Composition for enhancing respiratory tract immunity of animals, method and application thereof |
CN111388527A (en) * | 2020-04-10 | 2020-07-10 | 聊城创新畜禽养殖有限公司 | Preparation for preventing and treating mycoplasma of laying hens and preparation method thereof |
CN112618620A (en) * | 2020-07-20 | 2021-04-09 | 六安恒佳生物科技有限公司 | Solid-state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls and preparation method thereof |
CN114099590A (en) * | 2021-11-02 | 2022-03-01 | 江西新天地药业有限公司 | Fermented Chinese herbal medicine preparation for preventing and treating livestock and poultry diarrhea and preparation method thereof |
CN116585370A (en) * | 2023-03-21 | 2023-08-15 | 上海交通大学医学院附属第九人民医院 | Traditional Chinese medicine composition and application thereof in preparation of medicine for treating/preventing acute upper respiratory tract infection |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102805209A (en) * | 2012-08-30 | 2012-12-05 | 湖南省微生物研究所 | Synbiotics feed additive for beasts and birds and application thereof |
CN103704483A (en) * | 2013-12-31 | 2014-04-09 | 株洲千金药业股份有限公司 | Feed fermented by utilizing traditional Chinese medicine drugs and preparation method of feed |
-
2017
- 2017-06-08 CN CN201710427501.1A patent/CN107007640A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102805209A (en) * | 2012-08-30 | 2012-12-05 | 湖南省微生物研究所 | Synbiotics feed additive for beasts and birds and application thereof |
CN103704483A (en) * | 2013-12-31 | 2014-04-09 | 株洲千金药业股份有限公司 | Feed fermented by utilizing traditional Chinese medicine drugs and preparation method of feed |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109043159A (en) * | 2018-08-24 | 2018-12-21 | 广东群林牧业有限公司 | A kind of internal expelling parasite ewe feed formulation method |
CN111034855A (en) * | 2019-12-31 | 2020-04-21 | 山西大禹生物工程股份有限公司 | Composition for enhancing respiratory tract immunity of animals, method and application thereof |
CN111388527A (en) * | 2020-04-10 | 2020-07-10 | 聊城创新畜禽养殖有限公司 | Preparation for preventing and treating mycoplasma of laying hens and preparation method thereof |
CN112618620A (en) * | 2020-07-20 | 2021-04-09 | 六安恒佳生物科技有限公司 | Solid-state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls and preparation method thereof |
CN114099590A (en) * | 2021-11-02 | 2022-03-01 | 江西新天地药业有限公司 | Fermented Chinese herbal medicine preparation for preventing and treating livestock and poultry diarrhea and preparation method thereof |
CN116585370A (en) * | 2023-03-21 | 2023-08-15 | 上海交通大学医学院附属第九人民医院 | Traditional Chinese medicine composition and application thereof in preparation of medicine for treating/preventing acute upper respiratory tract infection |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107007640A (en) | A kind of production method for preventing and treating livestock and birds respiratory disease product | |
CN102366023B (en) | Method for preparing cow feed additive containing traditional Chinese medicine active probiotics | |
CN105174493B (en) | A kind of preparation method and applications of fermented type Chinese herbal medicine improver of water quality used for aquiculture | |
CN107594155A (en) | One kind improves milk production of cow biological feed additive | |
CN106306462A (en) | Probiotic fermented feed additive for pigs | |
CN102367416B (en) | Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive | |
CN101990992B (en) | Meat duck environmentally-friendly breeding feed additive | |
CN105876129B (en) | A kind of fermentation of Chinese herbal medicine preparation of alternative antibiotic and its preparation method and application | |
CN105432994A (en) | Pig fodder capable of improving daily body weight gain rate of pig and preparation method of pig fodder | |
CN104082528B (en) | A kind of feed addictive and preparation method thereof | |
CN105661028A (en) | Environmental-friendly biological pig feed additive | |
CN105410401A (en) | Pig feed capable of improving digestibility and preparation method of pig feed | |
CN104208239B (en) | A kind of for preventing and treating the Chinese medicine composition of poultry bacillus coli, microbial ecological agent and preparation method | |
CN103039700B (en) | Feed additive for veterinary drug solid fermentation and preparation method of feed additive | |
CN105724770A (en) | Chinese herbal medicine nanometer feed additive and preparing method and application thereof | |
CN103251913B (en) | Traditional Chinese medicine composition for protecting gastrointestinal tract of livestock and preparation method thereof | |
CN105461456A (en) | Pennisetum hydridum ecological organic fertilizer prepared from sheep manure and preparation method thereof | |
CN102367420B (en) | Traditional Chinese medicine probiotic liquid culture medium used in cow forage, culturing method, and forage additive | |
CN109589361A (en) | A kind of Chinese medicine composition for improving sow fertility rate, Chinese materia medica preparation and preparation method thereof | |
CN104222522A (en) | Chicken feed capable of preventing pullorum of black-bone chicken and preparation method of chicken feed | |
CN105410402A (en) | Pig feed capable of reducing breeding cost and preparation method of pig feed | |
CN107308415A (en) | One group is used to treat Chinese medicine composition of cad pig and preparation method thereof | |
CN111616102A (en) | Ecological goat breeding method in mountainous area | |
CN106359473A (en) | Fermented bacteria liquid capable of preventing and controlling pawpaw mosaic diseases and preparation method thereof | |
CN105961829A (en) | Chinese herbal medicinal micro-ecological premixed feed and preparation method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170804 |
|
RJ01 | Rejection of invention patent application after publication |