CN112618620A - Solid-state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls and preparation method thereof - Google Patents
Solid-state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a preparation method of a solid-state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls, which comprises the following steps: 1) weighing notopterygium root, divaricate saposhnikovia root, rhizoma atractylodis, fructus aurantii, mangnolia officinalis, asarum, ligusticum chuanxiong hort, radix angelicae and liquorice according to parts by weight; mixing and making into mixed powder; adding the mixed powder obtained in the step 1) into a solid state fermentation culture medium, uniformly mixing, inoculating probiotics, placing in an environment of 36-38 ℃, fermenting for 24-48 h, and after the fermentation is finished, spray drying the fermented product to obtain a solid state fermentation traditional Chinese medicine preparation; the action mechanism of the solid-state fermentation traditional Chinese medicine additive for controlling the occurrence of poultry respiratory diseases is as follows: the fermentation formula of the traditional Chinese medicine extract has the effects of eliminating inflammation, sweating, dispelling dampness, clearing internal heat, eliminating cold stress, enhancing disease resistance and preventing diseases in the bud.
Description
Technical Field
The invention relates to the technical field of feed additives, in particular to a solid-state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls and a preparation method thereof.
Background
The causes of diseases in the respiratory tract of poultry are many. For example, in winter and spring, the temperature is low, so that a large group of poultry can generate great stress. Under stress conditions, the body resistance of poultry is reduced, and various diseases are accompanied: 1. viral infectious diseases: infectious bronchitis, infectious laryngotracheitis, newcastle disease, avian influenza; 2. bacterial infectious diseases: mycoplasma, escherichia coli, staphylococcus, and the like; 3. and (3) mould: aspergillus; 4. the condition is as follows: poor ventilation, harmful gas NH3, and the like.
Current conventional practices for controlling respiratory tract disease: firstly, the vaccine is made regularly, immune antibodies are increased, and diseases are prevented; secondly, after the immune failure occurs, a large amount of antibiotics are used, the symptomatic treatment is carried out, and the death rate is reduced. With the use of a large amount of antibiotics, the external environment deteriorates, the organism generates drug resistance, and the treatment effect is gradually reduced.
Disclosure of Invention
The invention aims to solve the technical problem of providing a solid fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls and a preparation method thereof.
In order to solve the technical problems, the technical scheme of the invention is as follows: a method for preparing a solid fermentation Chinese medicinal additive for controlling respiratory diseases of egg-laying poultry,
the method comprises the following steps:
1) weighing notopterygium root, divaricate saposhnikovia root, rhizoma atractylodis, fructus aurantii, mangnolia officinalis, asarum, ligusticum chuanxiong hort, radix angelicae and liquorice according to parts by weight; mixing and making into mixed powder;
3) adding the mixed powder obtained in the step 1) into a solid state fermentation culture medium, uniformly mixing, inoculating probiotics, placing in an environment of 36-38 ℃, fermenting for 24-48 h, and after the fermentation is finished, spray drying the fermented product to obtain the solid state fermentation traditional Chinese medicine preparation.
Preferably, the adding ratio of the mixed powder to the solid fermentation medium is 8-20 g: 1L.
Preferably, the formulation of the solid state fermentation medium is: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5 g of glucose, 1 g of soluble starch, 5 g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water.
Preferably, the probiotic is bacillus subtilis, the strain is a secondary seed liquid, and the secondary seed liquid is prepared by the following method:
activating probiotic strains, inoculating the activated probiotic strains into a seed culture medium, placing the seed culture medium in an environment with the temperature of 36-38 ℃, and statically culturing the seed culture medium for 12-24 hours to obtain a first-stage seed solution; inoculating the primary seed solution into a seed culture medium according to the inoculation amount of 2-10 vol%, and placing the seed culture medium in an environment of 36-38 ℃ for static culture for 12-24 h to obtain a secondary seed solution.
Preferably, the seed culture medium of the first-stage seed liquid is the same as that of the second-stage seed liquid, and the formula is as follows: 10 g of refined peptone, 5 g of yeast extract, 20g of glucose, 5 g of sodium acetate, 2 g of ammonium citrate, 800.1 g of tween, 0.58 g of magnesium sulfate, 0.28 g of manganese sulfate and 1000ml of distilled water.
A solid-state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls is prepared from the following raw materials in parts by weight:
35-40 parts of notopterygium root, 25-30 parts of divaricate saposhnikovia root, 35-40 parts of rhizoma atractylodis, 20-25 parts of fructus aurantii, 20-25 parts of mangnolia officinalis, 15-20 parts of asarum, 20-25 parts of ligusticum chuanxiong hort, 20-25 parts of radix angelicae and 20-25 parts of liquorice.
Preferably, the feed additive is prepared from the following raw materials in parts by weight: 35 parts of notopterygium root, 25 parts of divaricate saposhnikovia root, 35 parts of rhizoma atractylodis, 20 parts of fructus aurantii, 20 parts of mangnolia officinalis, 15 parts of asarum, 20 parts of ligusticum chuanxiong hort, 20 parts of radix angelicae and 20 parts of liquorice.
The invention has the beneficial effects that:
1) notopterygium root, rhizoma Et radix Notopterygii, being pungent and warm in flavor, is the monarch drug because it is good at dispelling wind-cold-damp pathogen on the surface. Fang Feng enters jueyin, especially dispersing wind of the whole body; the rhizoma atractylodis is taken into the sun, the dehumidifying effect is strong, and the two ingredients help notopterygium root to sweat and remove rheumatism, and are used as ministerial drugs. Asarum herb, herba asari governs entering shaoyin, dispersing cold and unblocking collaterals, especially relieving pain; bai Zhi enters yang-brightness, dispels wind and cold, and can disperse arthralgia; chuan Xiong enters shao yang mainly and dispels wind to promote qi and blood circulation; fructus Aurantii is dry immature fruit of Citrus aurantium L.of Citrus of Rutaceae and its cultivar, has slightly cold nature, bitter and sour taste, and slightly cold nature, enters lung, spleen, stomach and large intestine channels, has effects of regulating qi-flowing, relieving epigastric distention, activating stagnancy and relieving flatulence, and is mainly used for treating qi stagnation in chest and hypochondrium, fullness and pain, dyspepsia, regulating qi-flowing, relieving epigastric distention, activating qi-flowing and relieving flatulence; hou Po is bitter and pungent in flavor and warm in nature, entering spleen, stomach and large intestine meridians. The functions of drying dampness, dispersing fullness and descending qi; the five flavors are used as adjuvant drugs for assisting monarch drugs and ministerial drugs in dispelling wind, dispelling cold and removing dampness to remove exterior pathogens and clearing and activating channels and collaterals to relieve pain. Prepared licorice root, radix Glycyrrhizae Praeparata, acts as a guiding drug to harmonize the effects of the other drugs in the recipe. The combination of the herbs can induce sweating and dispel dampness, dispel arthralgia and alleviate pain, and clear interior heat.
2) The practical application proves that the additive shows better clinical effect;
enhancing the immunity of the organism: the health-care food stimulates and mobilizes the immune defense system of the organism to play the role of antivirus, and is very in line with the traditional Chinese medicine that the vital qi exists in the interior and the pathogenic factors cannot be dried. The traditional Chinese medicines have important regulation effect on the immunity of the organism, and various traditional Chinese medicines act on the organism in a multi-layer, multi-path and multi-target way, identify the organism and eliminate the non-self components, thereby maintaining the stability of the internal environment of the organism and improving the immunity of the organism.
Promoting immune organ development: the immune organ is a group of mechanisms that generate immune responses and perform immune functions, and the quality of the immune organ is closely related to the immune functions.
The function of enhancing immune cells: the antivirus of Chinese medicine has different influence on T lymphocyte, killer cell, natural killer B cell, monocyte macrophage and leukocyte etc. immunocyte.
3) The action mechanism of the solid-state fermentation traditional Chinese medicine additive for controlling the occurrence of respiratory diseases of poultry is as follows: the fermentation formula of the traditional Chinese medicine extract has the effects of eliminating inflammation, sweating, dispelling dampness, clearing internal heat, eliminating cold stress, enhancing disease resistance and preventing diseases in the bud.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a graph of the rate at which respiratory symptoms appear in each group;
FIG. 2 is a graph showing the rate of change of the appearance of symptoms of chronic respiratory disease in each group during the test period.
Detailed Description
The following is a further description with reference to specific embodiments. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
The application provides a traditional Chinese medicine additive for poultry, which is prepared from the following raw materials in parts by weight: notopterygium root, divaricate saposhnikovia root, rhizoma atractylodis, fructus aurantii, magnolia officinalis, asarum, ligusticum wallichii, radix angelicae and liquorice.
Example one
A solid-state fermentation traditional Chinese medicine additive for controlling poultry respiratory diseases is prepared from the following traditional Chinese medicine raw material extracts in parts by weight: 35 parts of notopterygium root, 25 parts of divaricate saposhnikovia root, 35 parts of rhizoma atractylodis, 20 parts of fructus aurantii, 20 parts of mangnolia officinalis, 15 parts of asarum, 20 parts of ligusticum chuanxiong hort, 20 parts of radix angelicae and 20 parts of liquorice, and fermenting the traditional Chinese medicine extracts to form fermented traditional Chinese medicine powder;
the preparation method comprises the following steps:
1) weighing 35 parts of notopterygium root, 25 parts of divaricate saposhnikovia root, 35 parts of rhizoma atractylodis, 20 parts of fructus aurantii, 20 parts of mangnolia officinalis, 15 parts of asarum, 20 parts of ligusticum chuanxiong hort, 20 parts of radix angelicae and 20 parts of liquorice according to parts by weight to obtain mixed powder;
2) adding the mixed powder obtained in the step 1) into a solid fermentation culture medium, uniformly mixing to obtain a traditional Chinese medicine solid fermentation culture medium, inoculating probiotics, fermenting at 36 ℃ for 48h, stopping fermentation, and spray drying the fermented product to obtain the solid fermentation traditional Chinese medicine preparation. The adding ratio of the mixed powder to the solid fermentation medium is 20g to 1L.
The formula of the solid fermentation medium is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5 g of glucose, 1 g of soluble starch, 5 g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water.
Test cases
In 2019, 1 month, the layer chicken breeders in the town of the horse shop in Huqiu county, Anhui province, develop feeding tests. Wherein the number 1 greenhouse stockpile is 12000 Hailan brown laying hens (210 days old), which are divided into three groups, and each group comprises 4000. Experiment 1 group added a solid state fermentation Chinese medicinal additive for controlling poultry respiratory disease in winter and spring according to 1 kg/ton feed, and used for 30 days; experiment 2, antibiotics are added into the feed according to a conventional method; test 3 group did not have any additives added and belonged to blank group. By the end of 2019, month 2, the test was complete. The results are shown in the following table and in FIG. 1 (comparative curves of the rate of occurrence of respiratory disease in each group during the test period):
test results show that the application can effectively control respiratory diseases, and has obvious difference.
Example two
The traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw material extracts in parts by weight:
notopterygium root 40, ledebouriella root 30, atractylodes rhizome 40, bitter orange 25, magnolia bark 25, asarum herb 20, ligusticum wallichii 25, dahurian angelica root 25 and licorice root 25, and the fermented traditional Chinese medicine powder is prepared by fermenting the traditional Chinese medicine extracts.
The preparation method comprises the following steps:
1) weighing traditional Chinese medicine extracts of notopterygium root 40, divaricate saposhnikovia root 30, rhizoma atractylodis 40, fructus aurantii 25, magnolia officinalis 25, asarum 20, ligusticum chuanxiong hort 25, radix angelicae 25 and liquorice 25 in parts by weight;
2) adding the mixed powder obtained in the step 1) into a solid fermentation culture medium, uniformly mixing to obtain a traditional Chinese medicine solid fermentation culture medium, inoculating probiotics, fermenting at 36 ℃ for 48h, stopping fermentation, and spray drying the fermented product to obtain the solid fermentation traditional Chinese medicine preparation. The adding ratio of the mixed powder to the solid fermentation medium is 20g to 1L.
The formula of the solid fermentation medium is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5 g of glucose, 1 g of soluble starch, 5 g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water.
Test cases
In 2018, 1 month, the breeding test was carried out by the Zhongxing Zhengzhen laying hen breeder in Huqiu county, Anhui province. 6000 Hailan brown laying hens (320 days old) in No. 1 greenhouse are diagnosed as slow breathing when severe respiratory disease symptoms (cough and sneeze) appear at the beginning of the test. The whole group is divided into three groups of 2000 pieces. Experiment 1 group added a solid state fermentation Chinese medicinal additive for controlling poultry respiratory disease in winter and spring according to 1 kg/ton feed, and used for 30 days; in experiment 2, antibiotics are added according to the conventional requirements; test 3 group did not have any additives added and belonged to blank group. By the end of 2018, month 2, the test was complete. The results are shown in the following table and in FIG. 1 (the rate of change of respiratory symptoms in each group during the test period):
the test result shows that: when the laying hen group has a slow-exhaling symptom, the slow-exhaling symptom is effectively controlled and remarkably different after the traditional Chinese medicine additive is added into the laying hen feed.
The traditional Chinese medicine additive provided by the invention has the advantages that the traditional Chinese medicine is used as a fermentation raw material, the optimized bacillus subtilis is used for carrying out biotransformation on the intermediate substance, and the traditional Chinese medicine microecological preparation which is non-toxic, green, easy to absorb and obviously enhanced in antibacterial activity is prepared, so that the drug effect is obviously improved.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.
Claims (7)
1. A preparation method of a solid state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls is characterized in that: the method comprises the following steps:
1) weighing notopterygium root, divaricate saposhnikovia root, rhizoma atractylodis, fructus aurantii, mangnolia officinalis, asarum, ligusticum chuanxiong hort, radix angelicae and liquorice according to parts by weight; mixing and making into mixed powder;
2) adding the mixed powder obtained in the step 1) into a solid state fermentation culture medium, uniformly mixing, inoculating probiotics, placing in an environment of 36-38 ℃, fermenting for 24-48 h, and after the fermentation is finished, spray drying the fermented product to obtain the solid state fermentation traditional Chinese medicine preparation.
2. The preparation method of the solid state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls according to claim 1, wherein: the adding ratio of the mixed powder to the solid fermentation medium is 8-20 g: 1L.
3. The preparation method of the solid state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls according to claim 2, wherein: the formula of the solid fermentation medium is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5 g of glucose, 1 g of soluble starch, 5 g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water.
4. The preparation method of the solid state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls according to claim 3, wherein: the probiotic is bacillus subtilis, the strain is a secondary seed liquid, and the secondary seed liquid is prepared by the following method:
activating probiotic strains, inoculating the activated probiotic strains into a seed culture medium, placing the seed culture medium in an environment with the temperature of 36-38 ℃, and statically culturing the seed culture medium for 12-24 hours to obtain a first-stage seed solution; inoculating the primary seed solution into a seed culture medium according to the inoculation amount of 2-10 vol%, and placing the seed culture medium in an environment of 36-38 ℃ for static culture for 12-24 h to obtain a secondary seed solution.
5. The preparation method of the solid state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls according to claim 4, wherein: the seed culture medium of the first-stage seed liquid is the same as that of the second-stage seed liquid, and the formula is as follows: 10 g of refined peptone, 5 g of yeast extract, 20g of glucose, 5 g of sodium acetate, 2 g of ammonium citrate, 800.1 g of tween, 0.58 g of magnesium sulfate, 0.28 g of manganese sulfate and 1000ml of distilled water.
6. The Chinese medicinal additive prepared by the preparation method of the solid state fermentation Chinese medicinal additive for controlling the respiratory diseases of the laying fowl according to the claims 1 to 4 is characterized in that: the composition is prepared from the following raw materials in parts by weight:
35-40 parts of notopterygium root, 25-30 parts of divaricate saposhnikovia root, 35-40 parts of rhizoma atractylodis, 20-25 parts of fructus aurantii, 20-25 parts of mangnolia officinalis, 15-20 parts of asarum, 20-25 parts of ligusticum chuanxiong hort, 20-25 parts of radix angelicae and 20-25 parts of liquorice.
7. The traditional Chinese medicine additive according to claim 6, characterized in that: the composition is prepared from the following raw materials in parts by weight: 35 parts of notopterygium root, 25 parts of divaricate saposhnikovia root, 35 parts of rhizoma atractylodis, 20 parts of fructus aurantii, 20 parts of mangnolia officinalis, 15 parts of asarum, 20 parts of ligusticum chuanxiong hort, 20 parts of radix angelicae and 20 parts of liquorice.
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