CN112568324A - Composite probiotic fermented traditional Chinese medicine feed additive and preparation method and application thereof - Google Patents

Composite probiotic fermented traditional Chinese medicine feed additive and preparation method and application thereof Download PDF

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Publication number
CN112568324A
CN112568324A CN202011590563.2A CN202011590563A CN112568324A CN 112568324 A CN112568324 A CN 112568324A CN 202011590563 A CN202011590563 A CN 202011590563A CN 112568324 A CN112568324 A CN 112568324A
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chinese medicine
traditional chinese
feed additive
medicine composition
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CN112568324B (en
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金顺义
高淑娟
刘淑鹤
何柏江
王清玲
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Henan Senlong Animal Health Product Co ltd
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Henan Senlong Animal Health Product Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/02—Breeding vertebrates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/169—Plantarum
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/41—Pediococcus
    • A23V2400/413—Acidilactici
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention provides a composite probiotic fermented traditional Chinese medicine feed additive, a preparation method and application thereof, and relates to the field of feed additives. The feed additive is prepared by fermenting a traditional Chinese medicine composition and compound probiotics by using a modern biological fermentation technology, wherein the traditional Chinese medicine composition comprises: radix aucklandiae, pericarpium Citri Tangerinae, fructus Zanthoxyli, Glycyrrhrizae radix, rhizoma Alpiniae Officinarum, fructus Ligustri Lucidi, radix astragali, fructus Hippophae, flos Magnoliae officinalis, Poria, and fructus Alpinae Oxyphyllae; the composite probiotics comprise: bacillus licheniformis, pediococcus acidilactici, lactobacillus plantarum and fermented glutinous rice yeast. The composite probiotic fermented traditional Chinese medicine feed additive provided by the invention can obviously improve the feed intake of broiler chickens, reduce the feed-weight ratio and has obvious weight gain effect, effectively reduces the death rate during a test, can effectively improve the serum immune antibody level and immune organ index of the broiler chickens during the test, and has the beneficial effects of enhancing the body immunity and disease resistance.

Description

Composite probiotic fermented traditional Chinese medicine feed additive and preparation method and application thereof
Technical Field
The invention relates to the technical field of feed additives, in particular to a composite probiotic fermented traditional Chinese medicine feed additive, a preparation method thereof and application thereof in growth promotion of broiler chickens.
Background
The chicken has the advantages of high protein, low fat, easy digestion and absorption and the like, and gradually becomes one of the main meats consumed by residents in China. With the continuous improvement of market demands, broiler breeding gradually moves to a large-scale and highly intensive production mode. Meanwhile, the broiler chickens are always in a high-stress and high-sensitivity state in the whole feeding period, so that the production performance and the immunity of the broiler chickens are seriously influenced. It is common to add additives into feed to promote growth of broiler chickens and prevent and treat certain diseases, and most of the existing additives are chemically synthesized drugs, antibiotics, hormones and the like, so that the weight of the broiler chickens is increased while the broiler chickens contain drug residues, and certain toxic and side effects are generated, so that the broiler chickens are harmful to human health and life safety, and environmental pollution is caused. Along with the 194 th bulletin of Ministry of agricultural agriculture, the addition of antibacterial drugs except Chinese herbal medicines is totally forbidden in the feed of China from 1 month and 1 day of 2020. Therefore, the search for non-resistant and green additives for replacing antibiotics becomes the future development direction.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides the composite probiotic fermented traditional Chinese medicine feed additive and the preparation method and the application thereof.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme:
on one hand, the invention provides a composite probiotic fermented traditional Chinese medicine feed additive which comprises the following raw materials in parts by weight: 50-65 parts of a traditional Chinese medicine composition, 0.1-1 part of composite probiotic powder, 0-10 parts of soybean meal powder, 0.1-1 part of cellulase, 0-5 parts of brown sugar and 20-40 parts of purified water.
Further, as optimization of the compound probiotic fermented traditional Chinese medicine feed additive, the additive comprises the following raw materials in parts by weight: 55-65 parts of a traditional Chinese medicine composition, 0.5-1 part of composite probiotic powder, 5-10 parts of soybean meal, 0.1-0.5 part of cellulase, 2-4 parts of brown sugar and 30-35 parts of purified water.
Further, as optimization of the compound probiotic fermented traditional Chinese medicine feed additive, the additive comprises the following raw materials in parts by weight: 62.5 parts of a traditional Chinese medicine composition, 1 part of composite probiotic powder, 8 parts of soybean meal, 0.5 part of cellulase, 3 parts of brown sugar and 35 parts of purified water.
Further, as optimization of the compound probiotic fermented traditional Chinese medicine feed additive, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 1-10 parts of costustoot, 1-10 parts of orange peel, 1-10 parts of pepper, 1-10 parts of liquorice, 1-10 parts of galangal, 1-10 parts of glossy privet fruit, 1-10 parts of astragalus mongholicus, 1-10 parts of sea buckthorn, 1-10 parts of magnolia officinalis flower, 1-10 parts of poria cocos and 1-10 parts of fructus alpiniae oxyphyllae.
Further, as optimization of the compound probiotic fermented traditional Chinese medicine feed additive, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 5-10 parts of costustoot, 5-10 parts of orange peel, 5-10 parts of pepper, 5-10 parts of liquorice, 5-10 parts of galangal, 5-10 parts of glossy privet fruit, 5-10 parts of astragalus mongholicus, 5-10 parts of sea buckthorn, 5-10 parts of magnolia officinalis flower, 5-10 parts of poria cocos and 5-10 parts of fructus alpiniae oxyphyllae.
Further, as optimization of the compound probiotic fermented traditional Chinese medicine feed additive, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 5 parts of pepper, 6 parts of liquorice, 5 parts of galangal, 10 parts of glossy privet fruit, 8 parts of astragalus membranaceus, 10 parts of sea buckthorn, 5 parts of magnolia officinalis flower, 5 parts of poria cocos and 5 parts of fructus alpiniae oxyphyllae.
Further, as optimization of the compound probiotic fermented traditional Chinese medicine feed additive, the compound probiotic powder comprises: bacillus licheniformis, pediococcus acidilactici, lactobacillus plantarum and fermented glutinous rice yeast; wherein the bacillus licheniformis: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1-1.5: 1.5-2: 0.5-1: 1.5-2;
the viable bacteria number of each strain is as follows: bacillus licheniformis is more than or equal to 1..5 multiplied by 1010CFU/g, Pediococcus acidilactici is more than or equal to 7.5 multiplied by 109CFU/g, Lactobacillus plantarum greater than or equal to 1.0 × 1010CFU/g, Saccharomyces cerevisiae ≥ 5.5 × 109CFU/g。
On the other hand, the invention also provides a preparation method of the composite probiotic fermented traditional Chinese medicine feed additive, which comprises the following steps:
(1) weighing the following raw materials in parts by weight: 50-65 parts of a traditional Chinese medicine composition, 0-10 parts of soybean meal, 0-5 parts of brown sugar, 0.1-1 part of cellulase, 0-5 parts of composite probiotic powder and 20-40 parts of purified water;
(2) dissolving the composite probiotic powder, brown sugar and cellulase in purified water, fully and uniformly stirring, and carrying out closed culture at 37 ℃ for 24 hours to obtain activated bacteria liquid for later use;
(3) cleaning and drying the traditional Chinese medicine composition, crushing and sieving the traditional Chinese medicine composition by a 60-mesh sieve, and uniformly mixing to obtain a fermentation raw material for later use;
(4) and (3) fully mixing the activated bacterium liquid obtained in the step (2) and the fermentation raw material obtained in the step (3), uniformly stirring, placing in a sealed container, performing anaerobic sealed fermentation at the fermentation temperature of 30-40 ℃, the fermentation humidity of 30-40% and the fermentation time of 3-5 days, and fermenting to obtain the compound probiotic fermented traditional Chinese medicine feed additive.
The compound strain fermented traditional Chinese medicine feed additive provided by the invention is used for broiler chicken production and is added into broiler chicken feed according to the standard of 10 kg/T.
The feed additive of the invention has the following functions of effective components:
costustoot: pungent and bitter taste, warm nature; spleen, stomach, large intestine, triple energizer and gallbladder meridian entered; has effects in promoting qi circulation, relieving pain, invigorating spleen, and promoting digestion; the traditional Chinese medicine composition is mainly used for treating gastrointestinal diseases such as spleen and stomach qi stagnation syndrome, dysentery, tenesmus and the like in clinic; modern pharmacological studies show that the costus has the effects of relieving smooth muscle spasm, reducing blood pressure, resisting bacteria and the like, and also has certain activity in the aspects of cancer resistance, immunity, anti-inflammation and the like.
Orange peel: bitter and pungent taste, warm in nature; entering lung and spleen meridians; has the effects of regulating qi, invigorating spleen, eliminating dampness, and eliminating phlegm; is mainly used for treating abdominal distention, anorexia, vomiting, diarrhea, cough, excessive phlegm, etc.; from the aspect of traditional Chinese medicine pharmacology, the orange peel has the effects of inhibiting the smooth muscle of intestines and stomach, promoting the secretion of digestive juice, benefiting gallbladder, relaxing bronchial smooth muscle and resisting shock.
Licorice root: sweet in taste and neutral in nature; entering heart, lung, spleen and stomach meridians; has effects of invigorating five internal organs, harmonizing the other drugs, nourishing kidney, and moistening lung, contains glycyrrhizic acid and liquiritin as main ingredients, and can be used for improving weakness of spleen and stomach, listlessness, debilitation, and relieving cough and asthma.
Galangal rhizome: pungent taste and hot property; spleen and stomach meridians entered; has the effects of warming stomach, relieving vomit, dispelling cold and relieving pain; is mainly used for treating abdominal psychroalgia, stomach cold emesis, belch and acid regurgitation; the traditional Chinese medicine composition is mainly used for treating digestive tract system diseases such as dyspepsia, acid regurgitation and vomiting and gastric ulcer in clinic. Modern researches have separated various effective pharmacological components from galangal, mainly comprise volatile oil, flavonoids and diarylheptanoids, and have outstanding effects in resisting oxidation, resisting ulcer, inhibiting malignant tumor proliferation, diminishing inflammation and relieving pain.
Glossy privet fruit: the nature is flat; sweet and bitter in taste; entering liver and kidney meridians; has effects in nourishing yin, prolonging life, tonifying liver and kidney, clearing away heat, improving eyesight, blackening hair and beard; the yin-tonifying Chinese herbal medicines have the effects of nourishing liver and kidney and strengthening muscles and bones, are used for treating liver and kidney yin deficiency, yin deficiency and internal heat, waist and limb weakness, kidney deficiency and spermatorrhea, can improve the functions of animal reproductive systems and improve the disease resistance of organisms;
astragalus root: sweet, slightly warm, thin and dense in flavor, entering lung and spleen meridians. The main functional indications are as follows: tonify qi, strengthen superficies, expel toxin, expel pus, induce diuresis, and promote granulation. The single action of the herb is especially effective in tonifying qi and blood and enhancing the anti-stress and immune functions of the body.
Sea-buckthorn: the medicine is prepared from fructus Hippophae, which has effects of promoting blood circulation, dispelling blood stasis, eliminating phlegm, relieving chest stuffiness, invigorating spleen and stomach, promoting fluid production, quenching thirst, clearing heat, and relieving diarrhea, and can be used for treating various diseases; can be used for treating cough with excessive phlegm, dyspepsia, abdominal pain due to dyspepsia, traumatic injury, blood stasis, and amenorrhea; the superoxide dismutase contained in fructus Hippophae can effectively exert in vivo free radical effect, improve immunity, regulate immunocompetent cells of organism, and act as immunomodulator.
Magnolia flower: bitter taste, slightly warm nature, entering spleen and stomach meridians; has the effects of regulating qi and eliminating dampness, and can be used for treating chest and gastric cavity stuffiness, fullness, and food retention.
Tuckahoe, poria cocos: sweet and light taste, mild in nature; the heart, lung, spleen and kidney meridians are entered; has the effects of promoting diuresis, eliminating dampness, invigorating spleen, and calming heart; can be used for treating edema, oliguria, phlegm and fluid retention, dizziness, palpitation, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, and insomnia; poria is rich in various chemical components, wherein triterpenes and polysaccharides are main active substances of Poria, and modern pharmacological research shows that Poria has pharmacological activities of resisting tumor, regulating immunity, resisting inflammation, resisting oxidation, resisting aging, etc.
Intelligence development: warm in nature, pungent in flavor, entering spleen and kidney meridians; has effects in warming spleen, relieving diarrhea, arresting salivation, warming kidney, stopping nocturnal emission, and reducing urination; fructus Alpinae Oxyphyllae mainly contains active ingredients such as sesquiterpene, diheptane, and flavone, and has pharmacological effects of regulating urination, resisting bacteria, tumor, and oxidative stress.
B, bacillus licheniformis: the strain is a functional feeding microorganism which is bred and bred by a modern biotechnology and a special process. After entering the intestinal tract, the live bacteria can ensure that the intestinal tract is anoxic through the oxygen deprivation biological effect and have the inhibiting effect on pathogenic bacteria such as staphylococcus, escherichia coli and the like; the product has growth promoting effect on Bacillus bifidus, Lactobacillus, Bacteroides, and Streptococcus digestans, so as to regulate intestinal dysbacteriosis and maintain intestinal microecological balance.
Pediococcus acidilactici: is a gram-positive bacterium which can produce acid and bacteriocin, can effectively regulate the health of animal gastrointestinal tracts, maintain the balance of intestinal flora, has an antagonistic effect on pathogenic microorganisms in animals, can competitively inhibit the pathogenic microorganisms, enhances the immunologic function of animal organisms, and is a novel feeding microorganism.
Lactobacillus plantarum: the strain has strong lactic acid producing capacity and relatively strong lactic acid producing bacteriocin capacity. Can effectively inhibit the growth of pathogenic bacteria, promote the peristalsis of animal intestinal tracts and improve the digestion and absorption of feed.
And (3) saccharomyces cerevisiae: also known as baker's yeast or budding yeast. The reproduction method is budding reproduction, and has the advantages of short growth cycle, strong fermentation capacity, easy large-scale culture, and rich nutritional ingredients such as various proteins, amino acids, vitamins, bioactive substances, etc. due to easy culture. The saccharomyces cerevisiae belongs to facultative anaerobe, and can consume oxygen in the gastrointestinal tract of animals after entering the gastrointestinal tract of the animals to cause an anaerobic environment, thereby promoting the propagation of beneficial flora and improving the microecological balance of the digestive tract of the animals.
(III) advantageous effects
The invention provides a compound probiotic fermented traditional Chinese medicine feed additive, a preparation method thereof and application thereof in broiler production. After the traditional Chinese medicine composition is fermented by probiotics, plant cell walls of the traditional Chinese medicine composition can be further opened, so that a pre-digestion effect is achieved, the digestion time of the medicine in vivo is reduced, and the absorption and utilization rate of the medicine is improved; meanwhile, a large amount of active beneficial bacteria and unknown growth factors can be generated in the fermentation process, and the method is more beneficial to stabilizing the micro-ecological balance and health of the intestinal tract of the organism. The composite probiotic fermented traditional Chinese medicine feed additive provided by the invention can obviously improve the feed intake of broiler chickens, reduce the feed-weight ratio and has obvious weight gain effect, effectively reduces the death rate during a test, can effectively improve the serum immune antibody level and immune organ index of the broiler chickens during the test, and has the beneficial effects of enhancing the body immunity and disease resistance. The feed additive is convenient to operate in the production process, safe and environment-friendly, is a green nonreactive product, and can be widely applied to broiler breeding.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A composite probiotic fermented traditional Chinese medicine feed additive is prepared according to the following steps:
(1) weighing the following raw materials in parts by weight: 50 parts of a traditional Chinese medicine composition, 2 parts of soybean meal, 1 part of brown sugar, 0.1 part of cellulase, 0.1 part of composite probiotic powder and 25 parts of purified water;
(2) dissolving the composite probiotic powder, brown sugar and cellulase in purified water, fully and uniformly stirring, and carrying out closed culture at 37 ℃ for 24 hours to obtain activated bacteria liquid for later use; wherein the probiotic powder comprises: bacillus licheniformis, pediococcus acidilactici, lactobacillus plantarum and fermented glutinous rice yeast; wherein the bacillus licheniformis: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1.5:1.5:0.5: 1.5; the viable bacteria number of each strain is as follows: bacillus licheniformis is more than or equal to 1..5 multiplied by 1010CFU/g, Pediococcus acidilactici is more than or equal to 7.5 multiplied by 109CFU/g, Lactobacillus plantarum greater than or equal to 1.0 × 1010CFU/g, Saccharomyces cerevisiae ≥ 5.5 × 109CFU/g。
(3) Cleaning and drying the traditional Chinese medicine composition, crushing and sieving the traditional Chinese medicine composition by a 60-mesh sieve, and uniformly mixing to obtain a fermentation raw material for later use; the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 6 parts of costustoot, 4 parts of orange peel, 2 parts of pepper, 4 parts of liquorice, 6 parts of galangal, 8 parts of glossy privet fruit, 5 parts of astragalus membranaceus, 4 parts of sea buckthorn, 2 parts of magnolia officinalis flower, 4 parts of poria cocos and 4 parts of fructus alpiniae oxyphyllae.
(4) And (3) fully mixing the activated bacterium liquid obtained in the step (2) and the fermentation raw material obtained in the step (3), uniformly stirring, placing in a sealed container, performing anaerobic sealed fermentation at the fermentation temperature of 30-40 ℃, the fermentation humidity of 30-40% and the fermentation time of 3-5 days, and fermenting to obtain the compound probiotic fermented traditional Chinese medicine feed additive.
Example 2
This example differs from example 1 in that:
the composite probiotics comprise the following strains in parts by weight: b, bacillus licheniformis: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1:2:1: 2;
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 10 parts of costustoot, 10 parts of orange peel, 10 parts of pepper, 10 parts of liquorice, 10 parts of galangal, 10 parts of glossy privet fruit, 10 parts of astragalus membranaceus, 10 parts of sea buckthorn, 1-10 parts of magnolia officinalis flower, 10 parts of poria cocos and 10 parts of fructus alpiniae oxyphyllae.
Example 3
This example differs from example 1 in that:
the composite probiotics comprise the following strains in parts by weight: b, bacillus licheniformis: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1.2:1.8:0.8: 1.2;
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 5 parts of costustoot, 5 parts of orange peel, 5 parts of pepper, 5 parts of liquorice, 5 parts of galangal, 5 parts of glossy privet fruit, 5 parts of astragalus membranaceus, 5 parts of sea buckthorn, 5 parts of magnolia officinalis flower, 5 parts of poria cocos and 5 parts of fructus alpiniae oxyphyllae.
Example 4
This example differs from example 1 in that:
the composite probiotics comprise the following strains in parts by weight: b, bacillus licheniformis: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1.5:1.5:1: 1.5;
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 5 parts of pepper, 6 parts of liquorice, 5 parts of galangal, 10 parts of glossy privet fruit, 8 parts of astragalus membranaceus, 10 parts of sea buckthorn, 5 parts of magnolia officinalis flower, 5 parts of poria cocos and 5 parts of fructus alpiniae oxyphyllae.
Example 5
This example differs from example 4 in that:
weighing the following raw materials in parts by weight: 65 parts of a traditional Chinese medicine composition, 1 part of composite probiotic powder, 10 parts of soybean meal, 1 part of cellulase, 5 parts of brown sugar and 40 parts of purified water.
Example 6
This example differs from example 4 in that:
weighing the following raw materials in parts by weight: 55 parts of a traditional Chinese medicine composition, 0.5 part of composite probiotic powder, 5 parts of soybean meal, 0.1 part of cellulase, 4 parts of brown sugar and 35 parts of purified water.
Example 7
This example differs from example 4 in that:
weighing the following raw materials in parts by weight: 62.5 parts of a traditional Chinese medicine composition, 1 part of composite probiotic powder, 8 parts of soybean meal, 0.5 part of cellulase, 3 parts of brown sugar and 35 parts of purified water.
Comparative example 1
This comparative example differs from example 7 in that:
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 5 parts of pepper, 6 parts of liquorice, 5 parts of galangal, 10 parts of glossy privet fruit, 8 parts of astragalus membranaceus, 10 parts of sea buckthorn, 5 parts of magnolia flower and 5 parts of poria cocos.
Comparative example 2
This comparative example differs from example 7 in that:
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 6 parts of liquorice, 10 parts of glossy privet fruit, 8 parts of astragalus membranaceus, 5 parts of magnolia officinalis flower, 5 parts of poria cocos and 5 parts of fructus alpiniae oxyphyllae.
Comparative example 3
This comparative example differs from example 7 in that:
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 5 parts of pepper, 5 parts of galangal, 10 parts of glossy privet fruit, 8 parts of astragalus mongholicus, 10 parts of sea buckthorn and 5 parts of fructus alpiniae oxyphyllae.
Comparative example 5
This comparative example differs from example 7 in that:
the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 5 parts of pepper, 6 parts of liquorice, 5 parts of galangal, 10 parts of sea buckthorn, 5 parts of magnolia flower, 5 parts of poria cocos and 5 parts of fructus alpiniae oxyphyllae.
Comparative example 6
This comparative example differs from example 7 in that:
the composite probiotics comprise the following strains in parts by weight: b, bacillus licheniformis: the weight ratio of the fermented glutinous rice yeast is 1.5: 1.5.
Comparative example 7
This comparative example differs from example 7 in that:
the composite probiotics comprise the following strains in parts by weight: b, bacillus licheniformis: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1.5:1.5: 1.5.
Comparative example 8
This comparative example differs from example 7 in that:
the composite probiotics comprise the following strains in parts by weight: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1.5:1: 1.5.
Comparative example 9
Cleaning and drying a traditional Chinese medicine composition, crushing and sieving the traditional Chinese medicine composition with a 60-mesh sieve to obtain the traditional Chinese medicine additive, wherein the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 5 parts of pepper, 6 parts of liquorice, 5 parts of galangal, 10 parts of glossy privet fruit, 8 parts of astragalus membranaceus, 10 parts of sea buckthorn, 5 parts of magnolia officinalis flower, 5 parts of poria cocos and 5 parts of fructus alpiniae oxyphyllae.
Example 8
The components of the feed additive are obtained by screening and researching the components according to the traditional Chinese medicine theory, the modern fermentation technology and the combination of the growth characteristics of the broilers and comparing the feeding test effects of different components on the broilers.
Test method 1
1.1 design of the experiment
500 1 daily diet avitamine broilers are selected and randomly divided into 10 groups, namely a test group, a comparative group 1 to a comparative group 9 and 50 broilers in each group, wherein the test group is fed with the fermented traditional Chinese medicine feed additive prepared in the example 7, the comparative groups 1 to 9 are fed with the feed additives prepared in the comparative examples 1 to 9, the basic daily diet and 1% of the feed additive are adopted, free feeding and free drinking are realized during the test period, the feeding environment is ensured to be consistent, and the test period is 42 days.
2 measurement of production Properties
Body weight index 42 days old 19:00, stop feeding water, 43 days old 08:00, all weighed repeatedly. Broiler feed intake was recorded in each replicate of each group per day and the average feed intake per day (ADFI), Average Daily Gain (ADG) feed-to-meat ratio (F/G) was calculated.
Statistical analysis of data
Data analysis statistical tests were performed on all data using SPSS20.0, and the results are expressed as mean. + -. standard deviation, asP<0.05) Indicating significant difference.
4 results and analysis
Table 1 shows the results of the productivity measurements of the groups of broiler chickens after 42 days of the test. As can be seen from Table 1, the test groups were higher than the control groups 1-9 in terms of daily gain and feed intake; the material weight ratio of the test groups is lower than that of the comparison groups 1-9, and when any raw material in the invention is lacked or any strain in the invention is lacked, the daily gain is improved, but the effect is not obvious. Thus, the additive formulations of the test groups are the preferred formulations of the present invention.
TABLE 1 influence of different formula feed additives on the productivity of 1-42 day-old broilers
Grouping Daily gain/g Daily average food intake/g Material to weight ratio
Test group 55.03±1.30 100.21±7.52 1.82
Comparative 1 group 54.22±1.21 99.37±6.23 1.85
Comparative 2 group 53.13±1.23 98.26±6.01 1.85
Comparative 3 group 51.33±1.09 95.37±5.98 1.86
Comparative 4 group 52.02±1.11 97.22±6.29 1.87
Comparative 5 groups 52.79±1.46 99.15±6.55 1.88
Comparative 6 group 50.83±1.18 96.55±5.77 1.90
Comparative 7 group 51.09±1.02 97.13±6.03 1.90
Comparative 8 group 51.44±1.05 98.33±6.89 1.91
Comparative 9 group 49.37±1.09 95.36±6.21 1.93
Example 9
In order to further illustrate the influence of the fermented traditional Chinese medicine feed additive on the production performance and the immunity of the broiler chicken, an animal test is provided as an illustration below. The following fermented traditional Chinese medicine feed additives of the present invention were prepared according to example 7.
Test method 1
Selecting 600 1 daily diet avitamine broilers, randomly dividing into 3 groups, namely a blank group, a control group and a test group, wherein each group has 5 repetitions, and each repetition has 40 repetitions; wherein the blank group is fed with basal diet only; adding 20% chlortetracycline hydrochloride into the basic daily ration in the control group, wherein the addition amount is 200 g/ton; the experimental group fed the fermented traditional Chinese medicine feed additive prepared in example 7, and the basic ration and 1% feed additive were adopted. In the test period, on-line flat culture is adopted, only feeding and drinking water are adopted, longitudinal negative pressure ventilation is adopted, a hot blast stove is used for heating, and the feeding management and immunization program refers to the avine broiler feeding management manual.
2 index measurement and measurement method
2.1 measurement of production Properties
Body weight index 42 days old 19:00, stop feeding water, 43 days old 08:00, all weighed repeatedly. Broiler feed intake was recorded in each replicate of each group per day and the average feed intake per day (ADFI), Average Daily Gain (ADG) feed-to-meat ratio (F/G) was calculated.
Mortality = death/start x 100%.
2.2 measurement of serum immune index and organ index
Stopping feeding water at 19:00 of 42 days old, collecting blood and slaughtering (2 in each repetition) of veins under 08:00 wings at 43 days old, measuring serum immune indexes SigA, IgM and IgA, weighing thymus, spleen and bursa of Fabricius, and calculating viscera index.
Organ index = organ weight (g)/body weight (g) × 100%
3 data statistics and analysis
Data analysis statistical tests were performed on all data using SPSS20.0, and the results are expressed as mean. + -. standard deviation, asP<0.05) Indicating significant difference.
4 results and analysis
4.1 Effect of fermented traditional Chinese medicine feed additive of the invention on broiler chicken production performance
As can be seen from table 2, the final weight of the test group was significantly increased (P < 0.05) compared to the control group; compared with the control group, the content of the compound is improved to a certain extent (P > 0.05). Compared with the blank group, the daily gain of the test group is obviously higher than that of the blank group (P < 0.05) and the control group (P < 0.05), and the difference between the blank group and the control group is not obvious. Daily food intake was not significantly different between groups (P > 0.05). Compared with the blank group, the weight ratio of the test group material is obviously higher than that of the blank group (P < 0.05) and the control group (P < 0.05), and the difference between the blank group and the control group is not obvious. The difference between mortality rates was not significant (P > 0.05) for each group.
TABLE 2 influence of the fermented Chinese medicinal feed additive on the productivity of 1-42 day-old broilers
Figure DEST_PATH_IMAGE002
Note that the data in the same row are marked with different lower case letters to indicate significant difference (P < 0.05), and the following table is the same.
4.2 Effect of fermented traditional Chinese medicine feed additive of the invention on broiler serum antibody level
As can be seen from table 3, compared with the blank group, the content of SigA in the test group is significantly higher than that in the blank group (P < 0.01), and the content of SigA in the test group is significantly higher than that in the control group (P < 0.05); the control group contained significantly higher levels of SigA than the blank self-sufficiency (P < 0.05). Compared with the blank group, the content of IgG in the test group is obviously higher than that in the blank group (P < 0.05) and than that in the control group (P > 0.05); control IgG was higher than blank (P > 0.05). Compared with the blank group, the IgM content of the test group is obviously higher than that of the blank group (P < 0.05) and that of the control group (P < 0.05), and the difference between the blank group and the control group is not obvious.
TABLE 3 influence of fermented Chinese medicinal feed additive on serum antibody level of 1-42 day-old broiler chickens
Item Blank group Control group Test group
SigA(μg/mg) 35.03±1.23c 39.17±1.28b 43.25±1.33a
IgG(μg/mg) 543.73±18.35b 602.53±23ab 645.28±25a
IgM(μg/mg) 55.07±1.86b 58.23±2.25b 63.51±2.12a
4.3 the influence of the fermented traditional Chinese medicine feed additive on the immune organ index of the broilers
As can be seen from table 4, the thymus index of the test group was significantly higher than that of the blank group (P < 0.05) compared to the blank group; the thymus index of the test group is not significantly different from that of the control group (P > 0.05); the blank group was not significantly different from the control group (P > 0.05). Thymus index, not significantly different between groups (P > 0.05). Compared with the blank group, the bursal index of the test group is obviously higher than that of the blank group; the bursal index of the test group is obviously higher than that of the control group (P < 0.05); no significant difference between blank and control groups P > 0.05).
TABLE 4 influence of fermented Chinese medicinal feed additive on immune organ index of 1-42 day-old broiler chickens
Item Blank group Control group Test group
Index of thymus 2.97±0.21b 3.41±0.21ab 3.75±0.22a
Spleen index 1.38±0.11 1.49±0.09 1.61±0.13
Bursal index 1.62±0.11b 1.83±0.12b 2.01±0.15a
According to the test results, the fermented traditional Chinese medicine feed additive disclosed by the invention can be used for effectively reducing the feed-weight ratio, increasing the daily feed intake and daily weight gain when being used for feeding broiler chickens, has a positive effect on the production of the broiler chickens, can increase the antibody level of broiler chicken serum and the immune organ index of the broiler chickens, can replace the use of antibiotics, enhances the immune function of the broiler chickens and improves the disease resistance.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims (8)

1. The composite probiotic fermented traditional Chinese medicine feed additive is characterized by comprising the following raw materials in parts by weight: 50-65 parts of a traditional Chinese medicine composition, 0.1-1 part of composite probiotic powder, 0.1-1 part of cellulase, 0-10 parts of soybean meal, 0-5 parts of brown sugar and 20-40 parts of purified water.
2. The compound probiotic fermented traditional Chinese medicine feed additive according to claim 1, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 1-10 parts of costustoot, 1-10 parts of orange peel, 1-10 parts of pepper, 1-10 parts of liquorice, 1-10 parts of galangal, 1-10 parts of glossy privet fruit, 1-10 parts of astragalus mongholicus, 1-10 parts of sea buckthorn, 1-10 parts of magnolia officinalis flower, 1-10 parts of poria cocos and 1-10 parts of fructus alpiniae oxyphyllae.
3. The compound probiotic fermented traditional Chinese medicine feed additive according to claim 1, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 5-10 parts of costustoot, 5-10 parts of orange peel, 5-10 parts of pepper, 5-10 parts of liquorice, 5-10 parts of galangal, 5-10 parts of glossy privet fruit, 5-10 parts of astragalus mongholicus, 5-10 parts of sea buckthorn, 5-10 parts of magnolia officinalis flower, 5-10 parts of poria cocos and 5-10 parts of fructus alpiniae oxyphyllae.
4. The compound probiotic fermented traditional Chinese medicine feed additive according to claim 1, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of costustoot, 10 parts of orange peel, 5 parts of pepper, 6 parts of liquorice, 5 parts of galangal, 10 parts of glossy privet fruit, 8 parts of astragalus membranaceus, 10 parts of sea buckthorn, 5 parts of magnolia officinalis flower, 5 parts of poria cocos and 5 parts of fructus alpiniae oxyphyllae.
5. The compound probiotic fermented traditional Chinese medicine feed additive according to claim 1, wherein the compound probiotic powder comprises: bacillus licheniformis, pediococcus acidilactici, lactobacillus plantarum and fermented glutinous rice yeast; wherein the bacillus licheniformis: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1-1.5: 1.5-2: 0.5-1: 1.5-2; the viable bacteria number of each strain is as follows: bacillus licheniformis is more than or equal to 1..5 multiplied by 1010CFU/g, Pediococcus acidilactici is more than or equal to 7.5 multiplied by 109CFU/g, Lactobacillus plantarum greater than or equal to 1.0 × 1010CFU/g, Saccharomyces cerevisiae ≥ 5.5 × 109CFU/g。
6. The compound probiotic fermented traditional Chinese medicine feed additive according to claim 5, wherein the ratio of Bacillus licheniformis: pediococcus acidilactici: lactobacillus plantarum: the weight ratio of the fermented glutinous rice yeast is 1.5:1.5:1: 1.5.
7. A preparation method of a compound probiotic fermented traditional Chinese medicine feed additive is characterized by comprising the following steps:
(1) weighing the following raw materials in parts by weight: 50-65 parts of a traditional Chinese medicine composition, 0-10 parts of soybean meal, 0-5 parts of brown sugar, 0.1-1 part of cellulase, 0-5 parts of composite probiotic powder and 20-40 parts of purified water;
(2) dissolving the composite probiotic powder, brown sugar and cellulase in purified water, fully and uniformly stirring, and carrying out closed culture at 37 ℃ for 24 hours to obtain activated bacteria liquid for later use;
(3) cleaning and drying the traditional Chinese medicine composition, crushing and sieving the traditional Chinese medicine composition by a 60-mesh sieve, and uniformly mixing to obtain a fermentation raw material for later use;
(4) and (3) fully mixing the activated bacterium liquid obtained in the step (2) and the fermentation raw material obtained in the step (3), uniformly stirring, placing in a sealed container, performing anaerobic sealed fermentation at the fermentation temperature of 30-40 ℃, the fermentation humidity of 30-40% and the fermentation time of 3-5 days, and fermenting to obtain the compound probiotic fermented traditional Chinese medicine feed additive.
8. The application of the compound strain fermented traditional Chinese medicine feed additive as claimed in any one of claims 1 to 5, wherein the additive is used for broiler chicken production and is added into broiler chicken feed according to the standard of 10 kg/T.
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CN106360040A (en) * 2016-08-24 2017-02-01 广东海大集团股份有限公司畜牧水产研究中心 Composite Chinese herbal medicine and probiotics feed additive for improving growth property and immuno-functions of broiler chicken
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CN113812521A (en) * 2021-09-28 2021-12-21 易之泰生物科技（龙岩）有限公司 Traditional Chinese medicine microbial inoculum and preparation method and application thereof
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