CN113812521A - Traditional Chinese medicine microbial inoculum and preparation method and application thereof - Google Patents
Traditional Chinese medicine microbial inoculum and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention provides a traditional Chinese medicine microbial inoculum and a preparation method and application thereof, wherein the microbial inoculum comprises: probiotic bacteria liquid, traditional Chinese medicine extracts, vitamin additives and trace element additives. The traditional Chinese medicine microbial inoculum can obviously increase the average daily gain and average daily feed intake of the Putian black ducks and obviously reduce the feed conversion ratio; the average egg laying number and the average quality of single eggs of the Pu Tian black ducks are obviously increased; the thymus index, the bursal index, the spleen index and the serum IgG content of the Putian black ducks are obviously increased, the hatching rate of the Putian black duck eggs is obviously improved, and the mercury, lead and cadmium content of leg muscles of the Putian black ducks is obviously reduced. The invention can also obviously increase the average daily gain and the average daily feed intake of the pigs and obviously reduce the feed conversion ratio; obviously increases the slaughtering rate and the meat purity of the pigs, obviously reduces the cooling loss rate of the carcasses, and reduces the mercury, lead and cadmium content of the hind leg muscles of the pigs.
Description
Technical Field
The invention relates to the field of animal nutrient solution or functional feed, in particular to a traditional Chinese medicine microbial inoculum and a preparation method and application thereof.
Background
Animal food such as livestock and poultry meat not only is a high-quality protein source, but also provides abundant micronutrients for human bodies. With the continuous improvement of living standard of people, the intake of animal food of residents in China also increases year by year, and heavy metal contained in meat food can cause various damages to human health. Heavy metals in food mainly come from soil, industrial three wastes, food processing and packaging and other ways, and pollutants enter human bodies through the enrichment function of food chains and are accumulated in certain organs to cause chronic poisoning. Lead, cadmium, mercury and the like are always concerned by people as common heavy metal pollutants.
The problems of the prior art are as follows: microbial fermentation animal feed is reported more and more at home and abroad, but the use effect is poor due to high production cost or unstable quality. The nutrient solution added with single microorganism can not play multiple functions, and the combination of multiple microorganisms solves the problems that whether the living environment of the microorganism needs oxygen, the fermentation temperature, the fermentation pH value, the nutrient condition required by fermentation and the storage and transportation of the microorganism nutrient solution. The microbial animal nutrient solution comprises various beneficial intestinal bacteria, trace element additives, vitamin additives, traditional Chinese medicine extracts and the like, and can improve the steady state of intestinal flora of fed animals, improve the immunity and the digestion and absorption capacity of animals and reduce the heavy metal content of meat products. In addition, the application also provides a production, preservation and use method of the nutrient solution.
Disclosure of Invention
In order to solve the technical problems, the invention provides a traditional Chinese medicine microbial inoculum and a preparation method thereof, and an application feed and a preparation method thereof. The following technical scheme is adopted:
1. a traditional Chinese medicine microbial inoculum and a preparation method and application thereof comprise: probiotic bacteria liquid, traditional Chinese medicine extracts, vitamin additives and trace element additives.
Preferably, the probiotic bacteria liquid comprises: bifidobacterium (Bifidobacterium), Lactobacillus (lactic acid bacteria), Clostridium (Clostridium), Escherichia (Escherichia), Burkholderia (Burkholderia cepacia) and Gemmium (Gemmiger).
Preferably, the herbal extract comprises: rumex, radix astragali, placenta hominis, radix astragali, radix Puerariae, radix Dipsaci, Eucommiae cortex, radix rehmanniae Preparata, semen Cuscutae, herba Taraxaci, pericarpium Citri Tangerinae, rhizoma Dioscoreae, and caulis et folium Linderae.
Preferably, the vitamin supplement comprises: compound vitamin B, vitamin C, vitamin E, vitamin D and vitamin K.
Preferably, the trace element additive comprises: iron hydroxymethanesulfinate, zinc hydroxymethanesulfinate, sodium selenite, manganese sulfate, potassium iodate and copper sulfate.
2. A traditional Chinese medicine microbial inoculum and a preparation method and an application preparation method thereof comprise the following steps:
(1) preparing bacterial liquid: bifidobacterium (Bifidobacterium), Lactobacillus (lactic acid bacteria), Clostridium (Clostridium), Escherichia (Escherichia), Burkholderia (Burkholderia cepacia) and Gemmium (Gemmiger) were purchased from North Nanobian and Shanghai Carum laboratories. The cells were streaked separately on solid LB medium and cultured at 35 ℃ for 24 hours. And picking the colonies, performing shake culture in a liquid LB culture medium for 24 hours, and storing at 4 ℃.
(2) Preparing a traditional Chinese medicine extract: liquid A: 20g of trametes versicolor, 50g of astragalus, 20g of human placenta, 50g of astragalus, 100g of kudzu root, 50g of teasel root and 100g of eucommia bark, and 2L of purified water is added. B, liquid B: adding 50g of prepared rhizome of rehmannia, 20g of dodder, 150g of dandelion, 50g of dried orange peel, 100g of Chinese yam and 50g of caulis humifuse euphorbiae into 2L of absolute ethyl alcohol, extracting for 4 hours by a Soxhlet extractor, concentrating the extracting solution to 100ml, dispersing in 500ml of water, and dissolving by ultrasonic oscillation for later use.
(3) Preparing a vitamin additive: a liquid: dissolving 0.2g of compound vitamin B in 100mL of water, shaking and mixing uniformly for later use. b, liquid: dissolving 0.5g of vitamin C in 100mL of water, shaking and mixing uniformly for later use. c, liquid: dissolving 0.3g of vitamin E and vitamin D in 100mL of vegetable oil, shaking and mixing uniformly for later use. D, liquid: dissolving 0.1g of vitamin K in 100mL of water, shaking and mixing uniformly for later use.
(4) Preparing a trace element additive: solution A: 30mg of iron hydroxymethanesulfinate (30mg/L) and 20mg of zinc hydroxymethanesulfinate (20mg/L) were added into 1L of purified water, and mixed uniformly by shaking for later use. And B, liquid B: adding 15mg sodium selenite (15mg/L) into 1L purified water, shaking and mixing well for use. And C, liquid C: 5mg of manganese sulfate (5mg/L) and 1mg of copper sulfate (1mg/L) are added into 1L of purified water, and the mixture is shaken and mixed evenly for standby. And (3) liquid D: adding 2mg of potassium iodate (2mg/L) into 1L of purified water, and shaking and mixing uniformly for later use.
3. The microbial animal nutrient solution feed obviously increases the average daily gain and the average daily feed intake, and obviously reduces the feed conversion ratio.
4. The application of the traditional Chinese medicine microbial inoculum in improving the egg yield of the black ducks in the Putian is characterized in that the average egg yield and the average quality of single eggs are remarkably increased by the microbial animal nutrient solution feed.
5. The microbial animal nutrient solution feed obviously increases thymus index, bursal disease index, spleen index and serum IgG content.
6. The application of the traditional Chinese medicine microbial inoculum in improving the hatching rate of the Pu Tian black duck eggs, and the microbial animal nutrient solution feed remarkably increases the hatching rate of the Pu Tian black duck eggs.
7. The microbial animal nutrient solution feed obviously reduces the content of mercury, lead and cadmium in leg muscles of the Putian black ducks.
8. The microbial animal nutrient solution feed obviously increases the average daily gain and the average daily feed intake of pigs and obviously reduces the feed conversion ratio.
9. The microbial animal nutrient solution feed obviously increases the slaughtering rate and the meat cleaning rate of pigs and obviously reduces the carcass cooling loss rate.
10. The application of the traditional Chinese medicine microbial inoculum in reducing the heavy metal content of the pigs is characterized in that the microbial animal nutrient solution feed obviously reduces the mercury, lead and cadmium content of the hind leg muscles of the pigs.
Has the advantages that: the traditional Chinese medicine microbial inoculum, the preparation method and the application thereof and the prepared feed can obviously increase the average daily gain and average daily feed intake of the Pu-Tian black ducks and obviously reduce the feed conversion ratio; the average egg laying number and the average quality of single eggs of the Pu Tian black ducks are obviously increased; the thymus index, the bursal index, the spleen index and the serum IgG content of the Putian black ducks are obviously increased, the hatching rate of the Putian black duck eggs is obviously improved, and the mercury, lead and cadmium content of leg muscles of the Putian black ducks is obviously reduced. The invention can also obviously increase the average daily gain and the average daily feed intake of the pigs and obviously reduce the feed conversion ratio; obviously increases the slaughtering rate and the meat purity of the pigs, obviously reduces the cooling loss rate of the carcasses, and reduces the mercury, lead and cadmium content of the hind leg muscles of the pigs.
Drawings
FIG. 1 is a diagram showing the preparation process and effect evaluation of the microbial animal nutrient solution.
Detailed description of the invention
In order to make the objects, technical solutions and advantages of the present invention more apparent, the following detailed description of the embodiments of the present invention is provided in connection with the specific embodiments. The test methods in the following examples are all conventional test methods unless otherwise specified, and the test reagents and consumables described in the following examples are all available from conventional biochemical reagents company, unless otherwise specified. In order to make the objects, technical solutions and advantages of the present invention more apparent, the following detailed description of the embodiments of the present invention is provided in connection with the specific embodiments. Examples of these preferred embodiments are illustrated in the specific examples. It should be noted that, in order to avoid obscuring the technical solutions of the present invention with unnecessary details, only the technical solutions and/or processing steps closely related to the technical solutions of the present invention are shown in the embodiments, and other details that are not relevant are omitted.
Example 1
The embodiment provides a traditional Chinese medicine microbial inoculum, which comprises: probiotic bacteria liquid, traditional Chinese medicine extracts, vitamin additives and trace element additives.
Preferably, the probiotic bacteria liquid comprises: bifidobacterium (Bifidobacterium), Lactobacillus (lactic acid bacteria), Clostridium (Clostridium), Escherichia (Escherichia), Burkholderia (Burkholderia cepacia) and Gemmium (Gemmiger).
Preferably, the herbal extract comprises: rumex, radix astragali, placenta hominis, radix astragali, radix Puerariae, radix Dipsaci, Eucommiae cortex, radix rehmanniae Preparata, semen Cuscutae, herba Taraxaci, pericarpium Citri Tangerinae, rhizoma Dioscoreae, and caulis et folium Linderae.
Preferably, the vitamin supplement comprises: compound vitamin B, vitamin C, vitamin E, vitamin D and vitamin K.
Preferably, the trace element additive comprises: iron hydroxymethanesulfinate, zinc hydroxymethanesulfinate, sodium selenite, manganese sulfate, potassium iodate and copper sulfate.
Example 2
The embodiment provides a traditional Chinese medicine microbial inoculum and a preparation method thereof, and the preparation method comprises the following steps:
(1) preparing bacterial liquid: bifidobacterium (Bifidobacterium), Lactobacillus (lactic acid bacteria), Clostridium (Clostridium), Escherichia (Escherichia), Burkholderia (Burkholderia cepacia) and Gemmium (Gemmiger) were purchased from North Nanobian and Shanghai Carum laboratories. The cells were streaked separately on solid LB medium and cultured at 35 ℃ for 24 hours. And picking the colonies, performing shake culture in a liquid LB culture medium for 24 hours, and storing at 4 ℃.
(2) Preparing a traditional Chinese medicine extract: liquid A: 20g of trametes versicolor, 50g of astragalus, 20g of human placenta, 50g of astragalus, 100g of kudzu root, 50g of teasel root and 100g of eucommia bark are added with 2L of purified water, decocted for 1h at 90 ℃ and concentrated to 500 ml. B, liquid B: adding 50g of prepared rhizome of rehmannia, 20g of dodder, 150g of dandelion, 50g of dried orange peel, 100g of Chinese yam and 50g of caulis humifuse euphorbiae into 2L of absolute ethyl alcohol, extracting for 4 hours by a Soxhlet extractor, concentrating the extracting solution to 100ml, dispersing in 500ml of water, and dissolving by ultrasonic oscillation for later use.
(3) Preparing a vitamin additive: a liquid: dissolving 0.2g of compound vitamin B in 100mL of water, shaking and mixing uniformly for later use. b, liquid: dissolving 0.5g of vitamin C in 100mL of water, shaking and mixing uniformly for later use. c, liquid: dissolving 0.3g of vitamin E and vitamin D in 100mL of vegetable oil, shaking and mixing uniformly for later use. d, liquid: dissolving 0.1g of vitamin K in 100mL of water, shaking and mixing uniformly for later use.
(4) Preparing a trace element additive: solution A: 30mg of iron hydroxymethanesulfinate (30mg/L) and 20mg of zinc hydroxymethanesulfinate (20mg/L) were added into 1L of purified water, and mixed uniformly by shaking for later use. And B, liquid B: adding 15mg sodium selenite (15mg/L) into 1L purified water, shaking and mixing well for use. And C, liquid C: 5mg of manganese sulfate (5mg/L) and 1mg of copper sulfate (1mg/L) are added into 1L of purified water, and the mixture is shaken and mixed evenly for standby. And (3) liquid D: adding 2mg of potassium iodate (2mg/L) into 1L of purified water, and shaking and mixing uniformly for later use.
Example 3
The embodiment provides a traditional Chinese medicine microbial inoculum, a preparation method thereof and application of the traditional Chinese medicine microbial inoculum in feed production improvement of Pu Tian black ducks, and the method comprises the following steps:
1. experimental methods
Selecting 1 day-old Putian black ducks, randomly dividing the Putian black ducks into 2 groups, wherein each group has 3 repetitions, and each repetition has 8 ducks and total 48 ducks. Feeding basic feed (Tongwei stock common duck feed) and purified water to the control group; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. Feeding the Putian black ducks for 60 days, and calculating average daily gain, average daily feed intake and feed-meat ratio.
The pure water containing the bacteria liquid comprises: 100ml of Bifidobacterium (Bifidobacterium), 100ml of Lactobacillus (lactic acid bacteria), 100ml of Clostridium (Clostridium), 50ml of Escherichia (Escherichia), 50ml of Burkholderia cepacia (Burkholderia cepacia) and 50ml of Gemmir (Gemmiger) were mixed in the lL purified water.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 100ml of solution A, 80ml of solution B, 50ml of solution A, 50ml of solution B, 40ml of solution C, 10ml of solution D, 10ml of solution a, 10ml of solution B, 20ml of solution C and 20ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
The data collected every day is counted on the 60 th day of the experiment, and the statistics are shown in the table 1, so that the average daily gain and the average daily feed intake of the microbial animal nutrient solution feed are obviously increased, and the feed conversion ratio is obviously reduced.
TABLE 1 microbial animal nutrient solution feed for increasing the yield of Putian black ducks
| Control group | Experimental group | |
| Average daily gain (g/d) | 3.5±1.16 | 8.9±1.36** |
| Average daily food intake (g/d) | 56.25±12.18 | 145.14±15.82** |
| Feed conversion ratio (g/g) | 2.9±1.12 | 2.3±0.98** |
Example 4
The embodiment provides an application of a traditional Chinese medicine microbial inoculum in increasing the egg yield of Putian black ducks, which comprises the following steps:
1. experimental methods
Selecting 1-day-old Putian black ducks, randomly dividing the black ducks into 2 groups, wherein each group has 3 repetitions, and each repetition has 8 ducks for total 48 ducks. Feeding basic feed (Tongwei stock common duck feed) and purified water to the control group; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and fed with traditional Chinese medicine, vitamin and microelement feed. And (5) finishing the first egg laying period after feeding the ducks for 180 days, and calculating the average egg laying number and the average quality of single eggs.
The pure water containing the bacteria liquid comprises: 100ml of Bifidobacterium (Bifidobacterium), 100ml of Lactobacillus (lactic acid bacteria), 100ml of Clostridium (Clostridium), 50ml of Escherichia (Escherichia), 50ml of Burkholderia cepacia (Burkholderia cepacia) and 50ml of Gemmir (Gemmiger) were mixed in the lL purified water.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 100ml of solution A, 80ml of solution B, 50ml of solution A, 50ml of solution B, 40ml of solution C, 10ml of solution D, 10ml of solution a, 10ml of solution B, 20ml of solution C and 20ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
Data collected every day are counted on the 180 th day of the experiment, and the statistics are shown in table 2, so that the average egg laying number and the average quality of single eggs are remarkably increased by the microbial animal nutrient solution feed.
TABLE 2 microbial animal nutrient solution feed for increasing the egg production of Putian black ducks
| Control group | Experimental group | |
| Average number of eggs (g/egg) | 8.9±2.88 | 15.63±4.22** |
| Average quality (g) of single egg | 68.68±19.12 | 89.73±11.22** |
Example 5
The embodiment provides an application of a traditional Chinese medicine microbial inoculum in improving immunity of Putian black ducks, which comprises the following steps:
1. experimental methods
Selecting 1 day-old Putian black ducks, randomly dividing the Putian black ducks into 2 groups, wherein each group has 3 repetitions, and each repetition has 8 ducks and total 48 ducks. Feeding basic feed (Tongwei stock common duck feed) and purified water to the control group; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. Feeding Putian black ducks for 60 days, taking thymus, spleen and bursa of fabricius after slaughtering, taking serum to measure IgG (the kit is built by Nanjing according to the kit instruction).
The pure water containing the bacteria liquid comprises: 100ml of Bifidobacterium (Bifidobacterium), 100ml of Lactobacillus (lactic acid bacteria), 100ml of Clostridium (Clostridium), 50ml of Escherichia (Escherichia), 50ml of Burkholderia cepacia (Burkholderia cepacia) and 50ml of Gemmir (Gemmiger) were mixed in the lL purified water.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 100ml of solution A, 80ml of solution B, 50ml of solution A, 50ml of solution B, 40ml of solution C, 10ml of solution D, 10ml of solution a, 10ml of solution B, 20ml of solution C and 20ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
The data collected every day at 60 days of the experiment were counted, and as shown in table 3, the microbial animal nutrient solution feed significantly increased thymus index, bursal index, spleen index, and serum IgG content.
TABLE 3 microbial animal nutrient solution feed for enhancing the immunity of Putian black ducks
| Control group | Experimental group | |
| Index of thymus | 0.56±0.21 | 0.59±0.27** |
| Bursal index | 0.17±0.08 | 0.42±0.02** |
| Spleen index | 0.15±0.08 | 0.25±0.07* |
| IgG(ug/ml) | 25.83±7.23 | 45.29±9.88** |
Example 6
The embodiment provides an application of a traditional Chinese medicine microbial inoculum in increasing hatching rate of Pu Tian black duck eggs, which comprises the following steps:
1. experimental methods
Selecting 1 day-old Pu Tian black ducks, randomly dividing male and female halves into 2 groups, each group has 3 repetitions, and each repetition has 8 ducks (male and female halves) for total 48 ducks. Feeding basic feed (Tongwei stock common duck feed) and purified water to the control group; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. The Putian black ducks are bred for 180 days, and 20 duck eggs are collected in each group and placed in an incubator for hatching.
The pure water containing the bacteria liquid comprises: 100ml of Bifidobacterium (Bifidobacterium), 100ml of Lactobacillus (lactic acid bacteria), 100ml of Clostridium (Clostridium), 50ml of Escherichia (Escherichia), 50ml of Burkholderia cepacia (Burkholderia cepacia) and 50ml of Gemmir (Gemmiger) were mixed in the lL purified water.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 100ml of solution A, 80ml of solution B, 50ml of solution A, 50ml of solution B, 40ml of solution C, 10ml of solution D, 10ml of solution a, 10ml of solution B, 20ml of solution C and 20ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
Statistics as shown in table 4, the microbial animal nutrient solution feed significantly increased duck egg hatchability.
TABLE 4 microbial animal nutrient solution feed increase hatching rate of Putian black duck eggs
| Control group | Experimental group | |
| Hatching rate | 0.75±0.39 | 0.95±0.78* |
Example 7
The embodiment provides an application of a traditional Chinese medicine microbial inoculum in reduction of heavy metal content of Putian black ducks, which comprises the following steps:
1. experimental methods
Selecting 1 day-old Putian black ducks, randomly dividing the Putian black ducks into 2 groups, wherein each group has 3 repetitions, and each repetition has 8 ducks and total 48 ducks. Feeding basic feed (Tongwei stock common duck feed) and purified water to the control group; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. Feeding Putian black ducks for 60 days, taking the back leg muscles after slaughtering, and sending the muscles to a Xian Jia spectral to measure the content of mercury, lead and cadmium.
The pure water containing the bacteria liquid comprises: 100ml of Bifidobacterium (Bifidobacterium), 100ml of Lactobacillus (lactic acid bacteria), 100ml of Clostridium (Clostridium), 50ml of Escherichia (Escherichia), 50ml of Burkholderia cepacia (Burkholderia cepacia) and 50ml of Gemmir (Gemmiger) were mixed in the lL purified water.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 100ml of solution A, 80ml of solution B, 50ml of solution A, 50ml of solution B, 40ml of solution C, 10ml of solution D, 10ml of solution a, 10ml of solution B, 20ml of solution C and 20ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
The data collected every day is counted on the 60 th day of the experiment, and the statistics are shown in table 5, so that the contents of mercury, lead and cadmium in the microbial animal nutrient solution feed are obviously reduced.
TABLE 5 microbial animal nutrient solution feed for reducing heavy metal content of Putian black duck
| Control group | Experimental group | |
| Mercury mg/kg | 0.064±0.004 | 0.023±0.002** |
| Lead mg/kg | 0.057±0.005 | 0.012±0.004** |
| Cadmium mg/kg | 0.023±0.003 | 0.008±0.007** |
Example 8
The embodiment provides an application of a traditional Chinese medicine microbial inoculum for improving pig yield, which comprises the following steps:
1. experimental methods
Three varieties of Duroc pigs, Italian pigs and Racumm pigs which reach the marketing standard are selected, 20 varieties of each variety are randomly divided into a control group and an experimental group, and the number of the varieties is 60 in each half. The control group was fed with basal feed (Tongwei stock common pig feed) and purified water; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. And 3 weeks in the fattening period, recording data and calculating average daily gain, average daily feed intake and feed conversion ratio.
The pure water containing the bacteria liquid comprises: in lL purified water, 200ml of Bifidobacterium (Bifidobacterium), 200ml of Lactobacillus (lactic acid bacteria), 200ml of Clostridium (Clostridium), 100ml of Escherichia (Escherichia), 100ml of Burkholderia cepacia (Burkholderia cepacia) and 100ml of Gemmir (Gemmiger) were mixed.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 200ml of solution A, 80ml of solution B, 80ml of solution A, 80ml of solution B, 50ml of solution C, 20ml of solution D, 20ml of solution a, 20ml of solution B, 30ml of solution C and 30ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
The data collected every day are counted in 3 weeks of the experiment, and the statistics are shown in table 6, so that the average daily gain and the average daily feed intake of the microbial animal nutrient solution feed are obviously increased, and the feed conversion ratio is obviously reduced.
TABLE 6 microbial animal nutrient solution feed for increasing pig yield
Example 9
The embodiment provides a traditional Chinese medicine microbial inoculum, a preparation method thereof and application of the traditional Chinese medicine microbial inoculum for improving pig carcass quality, and the application comprises the following steps:
1. experimental methods
Three varieties of Duroc pigs, Italian pigs and Racumm pigs which reach the marketing standard are selected, 20 varieties of each variety are randomly divided into a control group and an experimental group, and the number of the varieties is 60 in each half. The control group was fed with basal feed (Tongwei stock common pig feed) and purified water; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. And 3 weeks in the fattening period, recording data and calculating the dressing percentage, the net meat percentage and the carcass cooling loss rate.
The pure water containing the bacteria liquid comprises: in lL purified water, 200ml of Bifidobacterium (Bifidobacterium), 200ml of Lactobacillus (lactic acid bacteria), 200ml of Clostridium (Clostridium), 100ml of Escherichia (Escherichia), 100ml of Burkholderia cepacia (Burkholderia cepacia) and 100ml of Gemmir (Gemmiger) were mixed.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 200ml of solution A, 80ml of solution B, 80ml of solution A, 80ml of solution B, 50ml of solution C, 20ml of solution D, 20ml of solution a, 20ml of solution B, 30ml of solution C and 30ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
The data collected every day are counted in the 3 rd week of the experiment, and the statistics are shown in the table 7, so that the slaughtering rate and the meat purity of the microbial animal nutrient solution feed are obviously increased, and the carcass cooling loss rate is obviously reduced.
TABLE 7 microbial animal nutrient solution feed for increasing pig yield
Example 10
The embodiment provides an application of a traditional Chinese medicine microbial inoculum for reducing heavy metal content in pork, which comprises the following steps:
1. experimental methods
Three varieties of Duroc pigs, Italian pigs and Racumm pigs which reach the marketing standard are selected, 20 varieties of each variety are randomly divided into a control group and an experimental group, and the number of the varieties is 60 in each half. The control group was fed with basal feed (Tongwei stock common pig feed) and purified water; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. And (5) taking the retreating muscle after slaughtering to measure the heavy metal content.
The pure water containing the bacteria liquid comprises: in lL purified water, 200ml of Bifidobacterium (Bifidobacterium), 200ml of Lactobacillus (lactic acid bacteria), 200ml of Clostridium (Clostridium), 100ml of Escherichia (Escherichia), 100ml of Burkholderia cepacia (Burkholderia cepacia) and 100ml of Gemmir (Gemmiger) were mixed.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 200ml of solution A, 80ml of solution B, 80ml of solution A, 80ml of solution B, 50ml of solution C, 20ml of solution D, 20ml of solution a, 20ml of solution B, 30ml of solution C and 30ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
Statistics as shown in table 8, the microbial animal nutrient solution feed significantly reduced the heavy metal content.
TABLE 8 reduction of pig heavy metal content by microbial animal nutrient solution feed
The foregoing is directed to embodiments of the present application and it is noted that numerous modifications and adaptations may be made by those skilled in the art without departing from the principles of the present application and are intended to be within the scope of the present application.
Claims (10)
1. A traditional Chinese medicine microbial inoculum is characterized by comprising: probiotic bacteria liquid, traditional Chinese medicine extracts, vitamin additives and trace element additives;
the probiotic bacterial liquid comprises: bifidobacteria (Bifidobacterium), lactobacilli (lactic acid bacteria), clostridia (Clostridium), Escherichia (Escherichia), Burkholderia cepacia, and Gemmir (Gemmiger);
the traditional Chinese medicine extract comprises: ganoderma lucidum, radix astragali, placenta hominis, radix astragali, radix Puerariae, radix Dipsaci, cortex Eucommiae, radix rehmanniae Preparata, semen Cuscutae, herba Taraxaci, pericarpium Citri Reticulatae, rhizoma Dioscoreae, and caulis Piperis Futokadsurae;
the vitamin additive comprises: compound vitamin B, vitamin C, vitamin E, vitamin D and vitamin K;
the trace element additive comprises: iron hydroxymethanesulfinate, zinc hydroxymethanesulfinate, sodium selenite, manganese sulfate, potassium iodate and copper sulfate.
2. A preparation method of a traditional Chinese medicine microbial inoculum is characterized by comprising the following steps:
(1) preparing bacterial liquid: bifidobacterium (Bifidobacterium), Lactobacillus (lactic acid bacteria), Clostridium (Clostridium), Escherichia (Escherichia), Burkholderia (Burkholderia cepacia) and Gemmir (Gemmiger) were purchased from North Nanobian and Shanghai Yingxin laboratories, and were separately streaked on solid LB medium, cultured at 35 deg.C for 24h, picked colonies were shake-cultured in liquid LB medium for 24h, stored at 4 deg.C,
(2) preparing a traditional Chinese medicine extract: liquid A: 20g of trametes versicolor, 50g of astragalus membranaceus, 20g of human placenta, 50g of astragalus membranaceus, 100g of radix puerariae, 50g of teasel roots and 100g of eucommia ulmoides, 2L of purified water and a solution B: adding radix rehmanniae Preparata 50g, semen Cuscutae 20g, herba Taraxaci 150g, pericarpium Citri Tangerinae 50g, rhizoma Dioscoreae 100g, and caulis et folium Piperis Futokadsurae 50g into 2L anhydrous alcohol, extracting with Soxhlet extractor for 4 hr, concentrating the extractive solution to 100ml, dispersing in 500ml water, dissolving with ultrasonic oscillation for use,
(3) preparing a vitamin additive: a liquid: dissolving 0.2g of compound vitamin B in 100mL of water, shaking and uniformly mixing for later use, wherein the liquid B: dissolving 0.5g of vitamin C in 100mL of water, shaking and uniformly mixing for later use, wherein the liquid C: dissolving 0.3g of vitamin E and 0.3g of vitamin D in 100mL of vegetable oil, shaking and mixing uniformly for later use, wherein the D liquid: dissolving 0.1g of vitamin K in 100mL of water, shaking and mixing uniformly for later use,
(4) preparing a trace element additive: solution A: adding 30mg of iron hydroxymethanesulfinate (30mg/L) and 20mg of zinc hydroxymethanesulfinate (20mg/L) into 1L of purified water, shaking and mixing uniformly for later use, wherein the solution B comprises the following components: adding 15mg of sodium selenite (15mg/L) into 1L of purified water, shaking and mixing uniformly for later use, wherein the solution C is: adding 5mg of manganese sulfate (5mg/L) and 1mg of copper sulfate (1mg/L) into 1L of purified water, shaking and uniformly mixing for later use, wherein the solution D is: adding 2mg of potassium iodate (2mg/L) into 1L of purified water, and shaking and mixing uniformly for later use.
3. The application of the traditional Chinese medicine microbial inoculum for improving the yield of the dark ducks in the Pu' er field, as claimed in claim 1, wherein the microbial animal nutrient solution feed has the advantages of obviously increasing the average daily gain and the average daily feed intake and obviously reducing the feed conversion ratio.
4. The application of the traditional Chinese medicine microbial inoculum for improving the egg yield of the dark duck in the Putian, as claimed in claim 1, wherein the microbial animal nutrient solution feed remarkably increases the average egg yield and the average quality of single egg.
5. The use of the traditional Chinese medicine microbial inoculum for improving the immunity of Putian black ducks as claimed in claim 1, wherein the microbial animal nutrient solution feed significantly increases thymus index, bursa of fabricius index, spleen index and serum IgG content.
6. The use of the traditional Chinese medicine microbial inoculum for improving the hatching rate of the black duck eggs in the Tata as claimed in claim 1, wherein the microbial animal nutrient solution feed significantly increases the hatching rate of the black duck eggs in the Tata.
7. The application of the traditional Chinese medicine microbial inoculum to reduce the heavy metal content of the Putian black ducks in claim 1, wherein the microbial animal nutrient solution feed remarkably reduces the mercury, lead and cadmium content of leg muscles of the Putian black ducks.
8. The application of the traditional Chinese medicine microbial inoculum for improving the pig yield according to claim 1, characterized in that the average daily gain and average daily feed intake of the pig are remarkably increased, and the feed conversion ratio is remarkably reduced.
9. The use of the traditional Chinese medicine microbial inoculum for improving the carcass quality of pigs as claimed in claim 1, wherein the microbial animal nutrient solution feed significantly increases the slaughtering rate and the meat purity of pigs and significantly reduces the carcass cooling loss rate.
10. The application of the traditional Chinese medicine microbial inoculum to reduce the heavy metal content of pigs according to claim 1, wherein the microbial animal nutrient solution feed remarkably reduces the mercury, lead and cadmium content of hind leg muscles of pigs.
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| CN112544812A (en) * | 2020-11-23 | 2021-03-26 | 陕西石羊农业科技股份有限公司 | Mixed-strain fermentation type Chinese herbal medicine additive for laying hens in later period of laying, and preparation method and application thereof |
| CN112568324A (en) * | 2020-12-29 | 2021-03-30 | 河南森隆动物保健品有限公司 | Composite probiotic fermented traditional Chinese medicine feed additive and preparation method and application thereof |
| RU2742866C1 (en) * | 2021-01-11 | 2021-02-11 | Общество с ограниченной ответственностью Научно-исследовательский центр "БОНАКА" | Fodder probiotic supplement for cattle |
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