CN113812521A - Traditional Chinese medicine microbial inoculum and preparation method and application thereof - Google Patents
Traditional Chinese medicine microbial inoculum and preparation method and application thereof Download PDFInfo
- Publication number
- CN113812521A CN113812521A CN202111140517.7A CN202111140517A CN113812521A CN 113812521 A CN113812521 A CN 113812521A CN 202111140517 A CN202111140517 A CN 202111140517A CN 113812521 A CN113812521 A CN 113812521A
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- traditional chinese
- vitamin
- feed
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003814 drug Substances 0.000 title claims abstract description 59
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 44
- 241000894006 Bacteria Species 0.000 claims abstract description 37
- 239000011573 trace mineral Substances 0.000 claims abstract description 26
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 26
- 235000013601 eggs Nutrition 0.000 claims abstract description 25
- 241000282887 Suidae Species 0.000 claims abstract description 23
- 239000000654 additive Substances 0.000 claims abstract description 20
- 230000001965 increasing effect Effects 0.000 claims abstract description 19
- 229940088594 vitamin Drugs 0.000 claims abstract description 18
- 229930003231 vitamin Natural products 0.000 claims abstract description 18
- 235000013343 vitamin Nutrition 0.000 claims abstract description 18
- 239000011782 vitamin Substances 0.000 claims abstract description 18
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 18
- 241000272525 Anas platyrhynchos Species 0.000 claims abstract description 17
- 229910052793 cadmium Inorganic materials 0.000 claims abstract description 12
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000006243 chemical reaction Methods 0.000 claims abstract description 12
- 235000021050 feed intake Nutrition 0.000 claims abstract description 12
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229910052753 mercury Inorganic materials 0.000 claims abstract description 12
- 210000003205 muscle Anatomy 0.000 claims abstract description 11
- 230000012447 hatching Effects 0.000 claims abstract description 10
- 239000000284 extract Substances 0.000 claims abstract description 9
- 238000003307 slaughter Methods 0.000 claims abstract description 8
- 235000013372 meat Nutrition 0.000 claims abstract description 7
- 239000006041 probiotic Substances 0.000 claims abstract description 7
- 230000000529 probiotic effect Effects 0.000 claims abstract description 7
- 235000018291 probiotics Nutrition 0.000 claims abstract description 7
- 210000000952 spleen Anatomy 0.000 claims abstract description 7
- 210000001541 thymus gland Anatomy 0.000 claims abstract description 7
- 238000001816 cooling Methods 0.000 claims abstract description 6
- 210000002966 serum Anatomy 0.000 claims abstract description 6
- 210000003141 lower extremity Anatomy 0.000 claims abstract description 4
- 241000272517 Anseriformes Species 0.000 claims abstract 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 67
- 241001465754 Metazoa Species 0.000 claims description 47
- 239000008213 purified water Substances 0.000 claims description 47
- 235000015097 nutrients Nutrition 0.000 claims description 46
- 230000000813 microbial effect Effects 0.000 claims description 34
- 241000186000 Bifidobacterium Species 0.000 claims description 28
- 241000588722 Escherichia Species 0.000 claims description 28
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 28
- 238000002156 mixing Methods 0.000 claims description 28
- 241000193403 Clostridium Species 0.000 claims description 27
- 241000589513 Burkholderia cepacia Species 0.000 claims description 22
- 241001185600 Gemmiger Species 0.000 claims description 14
- 241000186660 Lactobacillus Species 0.000 claims description 14
- 235000014655 lactic acid Nutrition 0.000 claims description 14
- 239000004310 lactic acid Substances 0.000 claims description 14
- 229910001385 heavy metal Inorganic materials 0.000 claims description 13
- 229940039696 lactobacillus Drugs 0.000 claims description 13
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 12
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 12
- 230000000996 additive effect Effects 0.000 claims description 10
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 6
- 239000009636 Huang Qi Substances 0.000 claims description 6
- 229930003270 Vitamin B Natural products 0.000 claims description 6
- 229930003268 Vitamin C Natural products 0.000 claims description 6
- 229930003316 Vitamin D Natural products 0.000 claims description 6
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 claims description 6
- 229930003427 Vitamin E Natural products 0.000 claims description 6
- 229930003448 Vitamin K Natural products 0.000 claims description 6
- 235000006533 astragalus Nutrition 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 6
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 6
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 6
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 6
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 6
- PHZPHBGQLGSMCQ-UHFFFAOYSA-L hydroxymethanesulfinate iron(2+) Chemical compound [Fe+2].OCS([O-])=O.OCS([O-])=O PHZPHBGQLGSMCQ-UHFFFAOYSA-L 0.000 claims description 6
- 229940099596 manganese sulfate Drugs 0.000 claims description 6
- 239000011702 manganese sulphate Substances 0.000 claims description 6
- 235000007079 manganese sulphate Nutrition 0.000 claims description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 6
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 claims description 6
- 210000002826 placenta Anatomy 0.000 claims description 6
- JLKDVMWYMMLWTI-UHFFFAOYSA-M potassium iodate Chemical compound [K+].[O-]I(=O)=O JLKDVMWYMMLWTI-UHFFFAOYSA-M 0.000 claims description 6
- 239000001230 potassium iodate Substances 0.000 claims description 6
- 235000006666 potassium iodate Nutrition 0.000 claims description 6
- 229940093930 potassium iodate Drugs 0.000 claims description 6
- 239000011781 sodium selenite Substances 0.000 claims description 6
- 229960001471 sodium selenite Drugs 0.000 claims description 6
- 235000015921 sodium selenite Nutrition 0.000 claims description 6
- 235000019156 vitamin B Nutrition 0.000 claims description 6
- 239000011720 vitamin B Substances 0.000 claims description 6
- 235000019154 vitamin C Nutrition 0.000 claims description 6
- 239000011718 vitamin C Substances 0.000 claims description 6
- 235000019166 vitamin D Nutrition 0.000 claims description 6
- 239000011710 vitamin D Substances 0.000 claims description 6
- 150000003710 vitamin D derivatives Chemical class 0.000 claims description 6
- 235000019165 vitamin E Nutrition 0.000 claims description 6
- 229940046009 vitamin E Drugs 0.000 claims description 6
- 239000011709 vitamin E Substances 0.000 claims description 6
- 235000019168 vitamin K Nutrition 0.000 claims description 6
- 239000011712 vitamin K Substances 0.000 claims description 6
- 150000003721 vitamin K derivatives Chemical class 0.000 claims description 6
- 229940046008 vitamin d Drugs 0.000 claims description 6
- 229940046010 vitamin k Drugs 0.000 claims description 6
- CAAIULQYGCAMCD-UHFFFAOYSA-L zinc;hydroxymethanesulfinate Chemical compound [Zn+2].OCS([O-])=O.OCS([O-])=O CAAIULQYGCAMCD-UHFFFAOYSA-L 0.000 claims description 6
- 241001453380 Burkholderia Species 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 230000036039 immunity Effects 0.000 claims description 4
- 210000000582 semen Anatomy 0.000 claims description 4
- 241000123589 Dipsacus Species 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 241000222355 Trametes versicolor Species 0.000 claims description 3
- 229960000355 copper sulfate Drugs 0.000 claims description 3
- 230000010355 oscillation Effects 0.000 claims description 3
- -1 placenta hominis Substances 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 3
- 239000008158 vegetable oil Substances 0.000 claims description 3
- 210000001669 bursa of fabricius Anatomy 0.000 claims description 2
- 241000045403 Astragalus propinquus Species 0.000 claims 2
- 241001112696 Clostridia Species 0.000 claims 1
- 241000208689 Eucommia ulmoides Species 0.000 claims 1
- 240000008397 Ganoderma lucidum Species 0.000 claims 1
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims 1
- 230000017448 oviposition Effects 0.000 abstract description 5
- 241000272522 Anas Species 0.000 description 37
- 238000002474 experimental method Methods 0.000 description 22
- 230000002354 daily effect Effects 0.000 description 17
- 244000005700 microbiome Species 0.000 description 12
- 239000002245 particle Substances 0.000 description 8
- 238000003825 pressing Methods 0.000 description 8
- 230000003203 everyday effect Effects 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 241001061264 Astragalus Species 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 210000004233 talus Anatomy 0.000 description 4
- 240000000467 Carum carvi Species 0.000 description 2
- 235000005747 Carum carvi Nutrition 0.000 description 2
- 240000007371 Cuscuta campestris Species 0.000 description 2
- 235000002722 Dioscorea batatas Nutrition 0.000 description 2
- 235000006536 Dioscorea esculenta Nutrition 0.000 description 2
- 240000001811 Dioscorea oppositifolia Species 0.000 description 2
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 description 2
- 241000208688 Eucommia Species 0.000 description 2
- 244000046146 Pueraria lobata Species 0.000 description 2
- 235000010575 Pueraria lobata Nutrition 0.000 description 2
- 241000405414 Rehmannia Species 0.000 description 2
- 241000219053 Rumex Species 0.000 description 2
- 240000001949 Taraxacum officinale Species 0.000 description 2
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000012676 herbal extract Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 235000019195 vitamin supplement Nutrition 0.000 description 2
- 208000027312 Bursal disease Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 231100000739 chronic poisoning Toxicity 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 235000021316 daily nutritional intake Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Inorganic Chemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a traditional Chinese medicine microbial inoculum and a preparation method and application thereof, wherein the microbial inoculum comprises: probiotic bacteria liquid, traditional Chinese medicine extracts, vitamin additives and trace element additives. The traditional Chinese medicine microbial inoculum can obviously increase the average daily gain and average daily feed intake of the Putian black ducks and obviously reduce the feed conversion ratio; the average egg laying number and the average quality of single eggs of the Pu Tian black ducks are obviously increased; the thymus index, the bursal index, the spleen index and the serum IgG content of the Putian black ducks are obviously increased, the hatching rate of the Putian black duck eggs is obviously improved, and the mercury, lead and cadmium content of leg muscles of the Putian black ducks is obviously reduced. The invention can also obviously increase the average daily gain and the average daily feed intake of the pigs and obviously reduce the feed conversion ratio; obviously increases the slaughtering rate and the meat purity of the pigs, obviously reduces the cooling loss rate of the carcasses, and reduces the mercury, lead and cadmium content of the hind leg muscles of the pigs.
Description
Technical Field
The invention relates to the field of animal nutrient solution or functional feed, in particular to a traditional Chinese medicine microbial inoculum and a preparation method and application thereof.
Background
Animal food such as livestock and poultry meat not only is a high-quality protein source, but also provides abundant micronutrients for human bodies. With the continuous improvement of living standard of people, the intake of animal food of residents in China also increases year by year, and heavy metal contained in meat food can cause various damages to human health. Heavy metals in food mainly come from soil, industrial three wastes, food processing and packaging and other ways, and pollutants enter human bodies through the enrichment function of food chains and are accumulated in certain organs to cause chronic poisoning. Lead, cadmium, mercury and the like are always concerned by people as common heavy metal pollutants.
The problems of the prior art are as follows: microbial fermentation animal feed is reported more and more at home and abroad, but the use effect is poor due to high production cost or unstable quality. The nutrient solution added with single microorganism can not play multiple functions, and the combination of multiple microorganisms solves the problems that whether the living environment of the microorganism needs oxygen, the fermentation temperature, the fermentation pH value, the nutrient condition required by fermentation and the storage and transportation of the microorganism nutrient solution. The microbial animal nutrient solution comprises various beneficial intestinal bacteria, trace element additives, vitamin additives, traditional Chinese medicine extracts and the like, and can improve the steady state of intestinal flora of fed animals, improve the immunity and the digestion and absorption capacity of animals and reduce the heavy metal content of meat products. In addition, the application also provides a production, preservation and use method of the nutrient solution.
Disclosure of Invention
In order to solve the technical problems, the invention provides a traditional Chinese medicine microbial inoculum and a preparation method thereof, and an application feed and a preparation method thereof. The following technical scheme is adopted:
1. a traditional Chinese medicine microbial inoculum and a preparation method and application thereof comprise: probiotic bacteria liquid, traditional Chinese medicine extracts, vitamin additives and trace element additives.
Preferably, the probiotic bacteria liquid comprises: bifidobacterium (Bifidobacterium), Lactobacillus (lactic acid bacteria), Clostridium (Clostridium), Escherichia (Escherichia), Burkholderia (Burkholderia cepacia) and Gemmium (Gemmiger).
Preferably, the herbal extract comprises: rumex, radix astragali, placenta hominis, radix astragali, radix Puerariae, radix Dipsaci, Eucommiae cortex, radix rehmanniae Preparata, semen Cuscutae, herba Taraxaci, pericarpium Citri Tangerinae, rhizoma Dioscoreae, and caulis et folium Linderae.
Preferably, the vitamin supplement comprises: compound vitamin B, vitamin C, vitamin E, vitamin D and vitamin K.
Preferably, the trace element additive comprises: iron hydroxymethanesulfinate, zinc hydroxymethanesulfinate, sodium selenite, manganese sulfate, potassium iodate and copper sulfate.
2. A traditional Chinese medicine microbial inoculum and a preparation method and an application preparation method thereof comprise the following steps:
(1) preparing bacterial liquid: bifidobacterium (Bifidobacterium), Lactobacillus (lactic acid bacteria), Clostridium (Clostridium), Escherichia (Escherichia), Burkholderia (Burkholderia cepacia) and Gemmium (Gemmiger) were purchased from North Nanobian and Shanghai Carum laboratories. The cells were streaked separately on solid LB medium and cultured at 35 ℃ for 24 hours. And picking the colonies, performing shake culture in a liquid LB culture medium for 24 hours, and storing at 4 ℃.
(2) Preparing a traditional Chinese medicine extract: liquid A: 20g of trametes versicolor, 50g of astragalus, 20g of human placenta, 50g of astragalus, 100g of kudzu root, 50g of teasel root and 100g of eucommia bark, and 2L of purified water is added. B, liquid B: adding 50g of prepared rhizome of rehmannia, 20g of dodder, 150g of dandelion, 50g of dried orange peel, 100g of Chinese yam and 50g of caulis humifuse euphorbiae into 2L of absolute ethyl alcohol, extracting for 4 hours by a Soxhlet extractor, concentrating the extracting solution to 100ml, dispersing in 500ml of water, and dissolving by ultrasonic oscillation for later use.
(3) Preparing a vitamin additive: a liquid: dissolving 0.2g of compound vitamin B in 100mL of water, shaking and mixing uniformly for later use. b, liquid: dissolving 0.5g of vitamin C in 100mL of water, shaking and mixing uniformly for later use. c, liquid: dissolving 0.3g of vitamin E and vitamin D in 100mL of vegetable oil, shaking and mixing uniformly for later use. D, liquid: dissolving 0.1g of vitamin K in 100mL of water, shaking and mixing uniformly for later use.
(4) Preparing a trace element additive: solution A: 30mg of iron hydroxymethanesulfinate (30mg/L) and 20mg of zinc hydroxymethanesulfinate (20mg/L) were added into 1L of purified water, and mixed uniformly by shaking for later use. And B, liquid B: adding 15mg sodium selenite (15mg/L) into 1L purified water, shaking and mixing well for use. And C, liquid C: 5mg of manganese sulfate (5mg/L) and 1mg of copper sulfate (1mg/L) are added into 1L of purified water, and the mixture is shaken and mixed evenly for standby. And (3) liquid D: adding 2mg of potassium iodate (2mg/L) into 1L of purified water, and shaking and mixing uniformly for later use.
3. The microbial animal nutrient solution feed obviously increases the average daily gain and the average daily feed intake, and obviously reduces the feed conversion ratio.
4. The application of the traditional Chinese medicine microbial inoculum in improving the egg yield of the black ducks in the Putian is characterized in that the average egg yield and the average quality of single eggs are remarkably increased by the microbial animal nutrient solution feed.
5. The microbial animal nutrient solution feed obviously increases thymus index, bursal disease index, spleen index and serum IgG content.
6. The application of the traditional Chinese medicine microbial inoculum in improving the hatching rate of the Pu Tian black duck eggs, and the microbial animal nutrient solution feed remarkably increases the hatching rate of the Pu Tian black duck eggs.
7. The microbial animal nutrient solution feed obviously reduces the content of mercury, lead and cadmium in leg muscles of the Putian black ducks.
8. The microbial animal nutrient solution feed obviously increases the average daily gain and the average daily feed intake of pigs and obviously reduces the feed conversion ratio.
9. The microbial animal nutrient solution feed obviously increases the slaughtering rate and the meat cleaning rate of pigs and obviously reduces the carcass cooling loss rate.
10. The application of the traditional Chinese medicine microbial inoculum in reducing the heavy metal content of the pigs is characterized in that the microbial animal nutrient solution feed obviously reduces the mercury, lead and cadmium content of the hind leg muscles of the pigs.
Has the advantages that: the traditional Chinese medicine microbial inoculum, the preparation method and the application thereof and the prepared feed can obviously increase the average daily gain and average daily feed intake of the Pu-Tian black ducks and obviously reduce the feed conversion ratio; the average egg laying number and the average quality of single eggs of the Pu Tian black ducks are obviously increased; the thymus index, the bursal index, the spleen index and the serum IgG content of the Putian black ducks are obviously increased, the hatching rate of the Putian black duck eggs is obviously improved, and the mercury, lead and cadmium content of leg muscles of the Putian black ducks is obviously reduced. The invention can also obviously increase the average daily gain and the average daily feed intake of the pigs and obviously reduce the feed conversion ratio; obviously increases the slaughtering rate and the meat purity of the pigs, obviously reduces the cooling loss rate of the carcasses, and reduces the mercury, lead and cadmium content of the hind leg muscles of the pigs.
Drawings
FIG. 1 is a diagram showing the preparation process and effect evaluation of the microbial animal nutrient solution.
Detailed description of the invention
In order to make the objects, technical solutions and advantages of the present invention more apparent, the following detailed description of the embodiments of the present invention is provided in connection with the specific embodiments. The test methods in the following examples are all conventional test methods unless otherwise specified, and the test reagents and consumables described in the following examples are all available from conventional biochemical reagents company, unless otherwise specified. In order to make the objects, technical solutions and advantages of the present invention more apparent, the following detailed description of the embodiments of the present invention is provided in connection with the specific embodiments. Examples of these preferred embodiments are illustrated in the specific examples. It should be noted that, in order to avoid obscuring the technical solutions of the present invention with unnecessary details, only the technical solutions and/or processing steps closely related to the technical solutions of the present invention are shown in the embodiments, and other details that are not relevant are omitted.
Example 1
The embodiment provides a traditional Chinese medicine microbial inoculum, which comprises: probiotic bacteria liquid, traditional Chinese medicine extracts, vitamin additives and trace element additives.
Preferably, the probiotic bacteria liquid comprises: bifidobacterium (Bifidobacterium), Lactobacillus (lactic acid bacteria), Clostridium (Clostridium), Escherichia (Escherichia), Burkholderia (Burkholderia cepacia) and Gemmium (Gemmiger).
Preferably, the herbal extract comprises: rumex, radix astragali, placenta hominis, radix astragali, radix Puerariae, radix Dipsaci, Eucommiae cortex, radix rehmanniae Preparata, semen Cuscutae, herba Taraxaci, pericarpium Citri Tangerinae, rhizoma Dioscoreae, and caulis et folium Linderae.
Preferably, the vitamin supplement comprises: compound vitamin B, vitamin C, vitamin E, vitamin D and vitamin K.
Preferably, the trace element additive comprises: iron hydroxymethanesulfinate, zinc hydroxymethanesulfinate, sodium selenite, manganese sulfate, potassium iodate and copper sulfate.
Example 2
The embodiment provides a traditional Chinese medicine microbial inoculum and a preparation method thereof, and the preparation method comprises the following steps:
(1) preparing bacterial liquid: bifidobacterium (Bifidobacterium), Lactobacillus (lactic acid bacteria), Clostridium (Clostridium), Escherichia (Escherichia), Burkholderia (Burkholderia cepacia) and Gemmium (Gemmiger) were purchased from North Nanobian and Shanghai Carum laboratories. The cells were streaked separately on solid LB medium and cultured at 35 ℃ for 24 hours. And picking the colonies, performing shake culture in a liquid LB culture medium for 24 hours, and storing at 4 ℃.
(2) Preparing a traditional Chinese medicine extract: liquid A: 20g of trametes versicolor, 50g of astragalus, 20g of human placenta, 50g of astragalus, 100g of kudzu root, 50g of teasel root and 100g of eucommia bark are added with 2L of purified water, decocted for 1h at 90 ℃ and concentrated to 500 ml. B, liquid B: adding 50g of prepared rhizome of rehmannia, 20g of dodder, 150g of dandelion, 50g of dried orange peel, 100g of Chinese yam and 50g of caulis humifuse euphorbiae into 2L of absolute ethyl alcohol, extracting for 4 hours by a Soxhlet extractor, concentrating the extracting solution to 100ml, dispersing in 500ml of water, and dissolving by ultrasonic oscillation for later use.
(3) Preparing a vitamin additive: a liquid: dissolving 0.2g of compound vitamin B in 100mL of water, shaking and mixing uniformly for later use. b, liquid: dissolving 0.5g of vitamin C in 100mL of water, shaking and mixing uniformly for later use. c, liquid: dissolving 0.3g of vitamin E and vitamin D in 100mL of vegetable oil, shaking and mixing uniformly for later use. d, liquid: dissolving 0.1g of vitamin K in 100mL of water, shaking and mixing uniformly for later use.
(4) Preparing a trace element additive: solution A: 30mg of iron hydroxymethanesulfinate (30mg/L) and 20mg of zinc hydroxymethanesulfinate (20mg/L) were added into 1L of purified water, and mixed uniformly by shaking for later use. And B, liquid B: adding 15mg sodium selenite (15mg/L) into 1L purified water, shaking and mixing well for use. And C, liquid C: 5mg of manganese sulfate (5mg/L) and 1mg of copper sulfate (1mg/L) are added into 1L of purified water, and the mixture is shaken and mixed evenly for standby. And (3) liquid D: adding 2mg of potassium iodate (2mg/L) into 1L of purified water, and shaking and mixing uniformly for later use.
Example 3
The embodiment provides a traditional Chinese medicine microbial inoculum, a preparation method thereof and application of the traditional Chinese medicine microbial inoculum in feed production improvement of Pu Tian black ducks, and the method comprises the following steps:
1. experimental methods
Selecting 1 day-old Putian black ducks, randomly dividing the Putian black ducks into 2 groups, wherein each group has 3 repetitions, and each repetition has 8 ducks and total 48 ducks. Feeding basic feed (Tongwei stock common duck feed) and purified water to the control group; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. Feeding the Putian black ducks for 60 days, and calculating average daily gain, average daily feed intake and feed-meat ratio.
The pure water containing the bacteria liquid comprises: 100ml of Bifidobacterium (Bifidobacterium), 100ml of Lactobacillus (lactic acid bacteria), 100ml of Clostridium (Clostridium), 50ml of Escherichia (Escherichia), 50ml of Burkholderia cepacia (Burkholderia cepacia) and 50ml of Gemmir (Gemmiger) were mixed in the lL purified water.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 100ml of solution A, 80ml of solution B, 50ml of solution A, 50ml of solution B, 40ml of solution C, 10ml of solution D, 10ml of solution a, 10ml of solution B, 20ml of solution C and 20ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
The data collected every day is counted on the 60 th day of the experiment, and the statistics are shown in the table 1, so that the average daily gain and the average daily feed intake of the microbial animal nutrient solution feed are obviously increased, and the feed conversion ratio is obviously reduced.
TABLE 1 microbial animal nutrient solution feed for increasing the yield of Putian black ducks
| Control group | Experimental group | |
| Average daily gain (g/d) | 3.5±1.16 | 8.9±1.36** |
| Average daily food intake (g/d) | 56.25±12.18 | 145.14±15.82** |
| Feed conversion ratio (g/g) | 2.9±1.12 | 2.3±0.98** |
Example 4
The embodiment provides an application of a traditional Chinese medicine microbial inoculum in increasing the egg yield of Putian black ducks, which comprises the following steps:
1. experimental methods
Selecting 1-day-old Putian black ducks, randomly dividing the black ducks into 2 groups, wherein each group has 3 repetitions, and each repetition has 8 ducks for total 48 ducks. Feeding basic feed (Tongwei stock common duck feed) and purified water to the control group; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and fed with traditional Chinese medicine, vitamin and microelement feed. And (5) finishing the first egg laying period after feeding the ducks for 180 days, and calculating the average egg laying number and the average quality of single eggs.
The pure water containing the bacteria liquid comprises: 100ml of Bifidobacterium (Bifidobacterium), 100ml of Lactobacillus (lactic acid bacteria), 100ml of Clostridium (Clostridium), 50ml of Escherichia (Escherichia), 50ml of Burkholderia cepacia (Burkholderia cepacia) and 50ml of Gemmir (Gemmiger) were mixed in the lL purified water.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 100ml of solution A, 80ml of solution B, 50ml of solution A, 50ml of solution B, 40ml of solution C, 10ml of solution D, 10ml of solution a, 10ml of solution B, 20ml of solution C and 20ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
Data collected every day are counted on the 180 th day of the experiment, and the statistics are shown in table 2, so that the average egg laying number and the average quality of single eggs are remarkably increased by the microbial animal nutrient solution feed.
TABLE 2 microbial animal nutrient solution feed for increasing the egg production of Putian black ducks
| Control group | Experimental group | |
| Average number of eggs (g/egg) | 8.9±2.88 | 15.63±4.22** |
| Average quality (g) of single egg | 68.68±19.12 | 89.73±11.22** |
Example 5
The embodiment provides an application of a traditional Chinese medicine microbial inoculum in improving immunity of Putian black ducks, which comprises the following steps:
1. experimental methods
Selecting 1 day-old Putian black ducks, randomly dividing the Putian black ducks into 2 groups, wherein each group has 3 repetitions, and each repetition has 8 ducks and total 48 ducks. Feeding basic feed (Tongwei stock common duck feed) and purified water to the control group; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. Feeding Putian black ducks for 60 days, taking thymus, spleen and bursa of fabricius after slaughtering, taking serum to measure IgG (the kit is built by Nanjing according to the kit instruction).
The pure water containing the bacteria liquid comprises: 100ml of Bifidobacterium (Bifidobacterium), 100ml of Lactobacillus (lactic acid bacteria), 100ml of Clostridium (Clostridium), 50ml of Escherichia (Escherichia), 50ml of Burkholderia cepacia (Burkholderia cepacia) and 50ml of Gemmir (Gemmiger) were mixed in the lL purified water.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 100ml of solution A, 80ml of solution B, 50ml of solution A, 50ml of solution B, 40ml of solution C, 10ml of solution D, 10ml of solution a, 10ml of solution B, 20ml of solution C and 20ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
The data collected every day at 60 days of the experiment were counted, and as shown in table 3, the microbial animal nutrient solution feed significantly increased thymus index, bursal index, spleen index, and serum IgG content.
TABLE 3 microbial animal nutrient solution feed for enhancing the immunity of Putian black ducks
| Control group | Experimental group | |
| Index of thymus | 0.56±0.21 | 0.59±0.27** |
| Bursal index | 0.17±0.08 | 0.42±0.02** |
| Spleen index | 0.15±0.08 | 0.25±0.07* |
| IgG(ug/ml) | 25.83±7.23 | 45.29±9.88** |
Example 6
The embodiment provides an application of a traditional Chinese medicine microbial inoculum in increasing hatching rate of Pu Tian black duck eggs, which comprises the following steps:
1. experimental methods
Selecting 1 day-old Pu Tian black ducks, randomly dividing male and female halves into 2 groups, each group has 3 repetitions, and each repetition has 8 ducks (male and female halves) for total 48 ducks. Feeding basic feed (Tongwei stock common duck feed) and purified water to the control group; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. The Putian black ducks are bred for 180 days, and 20 duck eggs are collected in each group and placed in an incubator for hatching.
The pure water containing the bacteria liquid comprises: 100ml of Bifidobacterium (Bifidobacterium), 100ml of Lactobacillus (lactic acid bacteria), 100ml of Clostridium (Clostridium), 50ml of Escherichia (Escherichia), 50ml of Burkholderia cepacia (Burkholderia cepacia) and 50ml of Gemmir (Gemmiger) were mixed in the lL purified water.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 100ml of solution A, 80ml of solution B, 50ml of solution A, 50ml of solution B, 40ml of solution C, 10ml of solution D, 10ml of solution a, 10ml of solution B, 20ml of solution C and 20ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
Statistics as shown in table 4, the microbial animal nutrient solution feed significantly increased duck egg hatchability.
TABLE 4 microbial animal nutrient solution feed increase hatching rate of Putian black duck eggs
| Control group | Experimental group | |
| Hatching rate | 0.75±0.39 | 0.95±0.78* |
Example 7
The embodiment provides an application of a traditional Chinese medicine microbial inoculum in reduction of heavy metal content of Putian black ducks, which comprises the following steps:
1. experimental methods
Selecting 1 day-old Putian black ducks, randomly dividing the Putian black ducks into 2 groups, wherein each group has 3 repetitions, and each repetition has 8 ducks and total 48 ducks. Feeding basic feed (Tongwei stock common duck feed) and purified water to the control group; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. Feeding Putian black ducks for 60 days, taking the back leg muscles after slaughtering, and sending the muscles to a Xian Jia spectral to measure the content of mercury, lead and cadmium.
The pure water containing the bacteria liquid comprises: 100ml of Bifidobacterium (Bifidobacterium), 100ml of Lactobacillus (lactic acid bacteria), 100ml of Clostridium (Clostridium), 50ml of Escherichia (Escherichia), 50ml of Burkholderia cepacia (Burkholderia cepacia) and 50ml of Gemmir (Gemmiger) were mixed in the lL purified water.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 100ml of solution A, 80ml of solution B, 50ml of solution A, 50ml of solution B, 40ml of solution C, 10ml of solution D, 10ml of solution a, 10ml of solution B, 20ml of solution C and 20ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
The data collected every day is counted on the 60 th day of the experiment, and the statistics are shown in table 5, so that the contents of mercury, lead and cadmium in the microbial animal nutrient solution feed are obviously reduced.
TABLE 5 microbial animal nutrient solution feed for reducing heavy metal content of Putian black duck
| Control group | Experimental group | |
| Mercury mg/kg | 0.064±0.004 | 0.023±0.002** |
| Lead mg/kg | 0.057±0.005 | 0.012±0.004** |
| Cadmium mg/kg | 0.023±0.003 | 0.008±0.007** |
Example 8
The embodiment provides an application of a traditional Chinese medicine microbial inoculum for improving pig yield, which comprises the following steps:
1. experimental methods
Three varieties of Duroc pigs, Italian pigs and Racumm pigs which reach the marketing standard are selected, 20 varieties of each variety are randomly divided into a control group and an experimental group, and the number of the varieties is 60 in each half. The control group was fed with basal feed (Tongwei stock common pig feed) and purified water; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. And 3 weeks in the fattening period, recording data and calculating average daily gain, average daily feed intake and feed conversion ratio.
The pure water containing the bacteria liquid comprises: in lL purified water, 200ml of Bifidobacterium (Bifidobacterium), 200ml of Lactobacillus (lactic acid bacteria), 200ml of Clostridium (Clostridium), 100ml of Escherichia (Escherichia), 100ml of Burkholderia cepacia (Burkholderia cepacia) and 100ml of Gemmir (Gemmiger) were mixed.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 200ml of solution A, 80ml of solution B, 80ml of solution A, 80ml of solution B, 50ml of solution C, 20ml of solution D, 20ml of solution a, 20ml of solution B, 30ml of solution C and 30ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
The data collected every day are counted in 3 weeks of the experiment, and the statistics are shown in table 6, so that the average daily gain and the average daily feed intake of the microbial animal nutrient solution feed are obviously increased, and the feed conversion ratio is obviously reduced.
TABLE 6 microbial animal nutrient solution feed for increasing pig yield
Example 9
The embodiment provides a traditional Chinese medicine microbial inoculum, a preparation method thereof and application of the traditional Chinese medicine microbial inoculum for improving pig carcass quality, and the application comprises the following steps:
1. experimental methods
Three varieties of Duroc pigs, Italian pigs and Racumm pigs which reach the marketing standard are selected, 20 varieties of each variety are randomly divided into a control group and an experimental group, and the number of the varieties is 60 in each half. The control group was fed with basal feed (Tongwei stock common pig feed) and purified water; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. And 3 weeks in the fattening period, recording data and calculating the dressing percentage, the net meat percentage and the carcass cooling loss rate.
The pure water containing the bacteria liquid comprises: in lL purified water, 200ml of Bifidobacterium (Bifidobacterium), 200ml of Lactobacillus (lactic acid bacteria), 200ml of Clostridium (Clostridium), 100ml of Escherichia (Escherichia), 100ml of Burkholderia cepacia (Burkholderia cepacia) and 100ml of Gemmir (Gemmiger) were mixed.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 200ml of solution A, 80ml of solution B, 80ml of solution A, 80ml of solution B, 50ml of solution C, 20ml of solution D, 20ml of solution a, 20ml of solution B, 30ml of solution C and 30ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
The data collected every day are counted in the 3 rd week of the experiment, and the statistics are shown in the table 7, so that the slaughtering rate and the meat purity of the microbial animal nutrient solution feed are obviously increased, and the carcass cooling loss rate is obviously reduced.
TABLE 7 microbial animal nutrient solution feed for increasing pig yield
Example 10
The embodiment provides an application of a traditional Chinese medicine microbial inoculum for reducing heavy metal content in pork, which comprises the following steps:
1. experimental methods
Three varieties of Duroc pigs, Italian pigs and Racumm pigs which reach the marketing standard are selected, 20 varieties of each variety are randomly divided into a control group and an experimental group, and the number of the varieties is 60 in each half. The control group was fed with basal feed (Tongwei stock common pig feed) and purified water; the experimental group is a microorganism animal nutrient solution group, and is fed with bacteria-containing purified water and traditional Chinese medicine and trace element feed. And (5) taking the retreating muscle after slaughtering to measure the heavy metal content.
The pure water containing the bacteria liquid comprises: in lL purified water, 200ml of Bifidobacterium (Bifidobacterium), 200ml of Lactobacillus (lactic acid bacteria), 200ml of Clostridium (Clostridium), 100ml of Escherichia (Escherichia), 100ml of Burkholderia cepacia (Burkholderia cepacia) and 100ml of Gemmir (Gemmiger) were mixed.
The traditional Chinese medicine, vitamin and trace element feed comprises: crushing 1kg of basic feed, adding 200ml of solution A, 80ml of solution B, 80ml of solution A, 80ml of solution B, 50ml of solution C, 20ml of solution D, 20ml of solution a, 20ml of solution B, 30ml of solution C and 30ml of solution D, adding a proper amount of purified water, uniformly mixing, and re-pressing feed particles with the sizes of members by adopting a granulator.
2. Results of the experiment
Statistics as shown in table 8, the microbial animal nutrient solution feed significantly reduced the heavy metal content.
TABLE 8 reduction of pig heavy metal content by microbial animal nutrient solution feed
The foregoing is directed to embodiments of the present application and it is noted that numerous modifications and adaptations may be made by those skilled in the art without departing from the principles of the present application and are intended to be within the scope of the present application.
Claims (10)
1. A traditional Chinese medicine microbial inoculum is characterized by comprising: probiotic bacteria liquid, traditional Chinese medicine extracts, vitamin additives and trace element additives;
the probiotic bacterial liquid comprises: bifidobacteria (Bifidobacterium), lactobacilli (lactic acid bacteria), clostridia (Clostridium), Escherichia (Escherichia), Burkholderia cepacia, and Gemmir (Gemmiger);
the traditional Chinese medicine extract comprises: ganoderma lucidum, radix astragali, placenta hominis, radix astragali, radix Puerariae, radix Dipsaci, cortex Eucommiae, radix rehmanniae Preparata, semen Cuscutae, herba Taraxaci, pericarpium Citri Reticulatae, rhizoma Dioscoreae, and caulis Piperis Futokadsurae;
the vitamin additive comprises: compound vitamin B, vitamin C, vitamin E, vitamin D and vitamin K;
the trace element additive comprises: iron hydroxymethanesulfinate, zinc hydroxymethanesulfinate, sodium selenite, manganese sulfate, potassium iodate and copper sulfate.
2. A preparation method of a traditional Chinese medicine microbial inoculum is characterized by comprising the following steps:
(1) preparing bacterial liquid: bifidobacterium (Bifidobacterium), Lactobacillus (lactic acid bacteria), Clostridium (Clostridium), Escherichia (Escherichia), Burkholderia (Burkholderia cepacia) and Gemmir (Gemmiger) were purchased from North Nanobian and Shanghai Yingxin laboratories, and were separately streaked on solid LB medium, cultured at 35 deg.C for 24h, picked colonies were shake-cultured in liquid LB medium for 24h, stored at 4 deg.C,
(2) preparing a traditional Chinese medicine extract: liquid A: 20g of trametes versicolor, 50g of astragalus membranaceus, 20g of human placenta, 50g of astragalus membranaceus, 100g of radix puerariae, 50g of teasel roots and 100g of eucommia ulmoides, 2L of purified water and a solution B: adding radix rehmanniae Preparata 50g, semen Cuscutae 20g, herba Taraxaci 150g, pericarpium Citri Tangerinae 50g, rhizoma Dioscoreae 100g, and caulis et folium Piperis Futokadsurae 50g into 2L anhydrous alcohol, extracting with Soxhlet extractor for 4 hr, concentrating the extractive solution to 100ml, dispersing in 500ml water, dissolving with ultrasonic oscillation for use,
(3) preparing a vitamin additive: a liquid: dissolving 0.2g of compound vitamin B in 100mL of water, shaking and uniformly mixing for later use, wherein the liquid B: dissolving 0.5g of vitamin C in 100mL of water, shaking and uniformly mixing for later use, wherein the liquid C: dissolving 0.3g of vitamin E and 0.3g of vitamin D in 100mL of vegetable oil, shaking and mixing uniformly for later use, wherein the D liquid: dissolving 0.1g of vitamin K in 100mL of water, shaking and mixing uniformly for later use,
(4) preparing a trace element additive: solution A: adding 30mg of iron hydroxymethanesulfinate (30mg/L) and 20mg of zinc hydroxymethanesulfinate (20mg/L) into 1L of purified water, shaking and mixing uniformly for later use, wherein the solution B comprises the following components: adding 15mg of sodium selenite (15mg/L) into 1L of purified water, shaking and mixing uniformly for later use, wherein the solution C is: adding 5mg of manganese sulfate (5mg/L) and 1mg of copper sulfate (1mg/L) into 1L of purified water, shaking and uniformly mixing for later use, wherein the solution D is: adding 2mg of potassium iodate (2mg/L) into 1L of purified water, and shaking and mixing uniformly for later use.
3. The application of the traditional Chinese medicine microbial inoculum for improving the yield of the dark ducks in the Pu' er field, as claimed in claim 1, wherein the microbial animal nutrient solution feed has the advantages of obviously increasing the average daily gain and the average daily feed intake and obviously reducing the feed conversion ratio.
4. The application of the traditional Chinese medicine microbial inoculum for improving the egg yield of the dark duck in the Putian, as claimed in claim 1, wherein the microbial animal nutrient solution feed remarkably increases the average egg yield and the average quality of single egg.
5. The use of the traditional Chinese medicine microbial inoculum for improving the immunity of Putian black ducks as claimed in claim 1, wherein the microbial animal nutrient solution feed significantly increases thymus index, bursa of fabricius index, spleen index and serum IgG content.
6. The use of the traditional Chinese medicine microbial inoculum for improving the hatching rate of the black duck eggs in the Tata as claimed in claim 1, wherein the microbial animal nutrient solution feed significantly increases the hatching rate of the black duck eggs in the Tata.
7. The application of the traditional Chinese medicine microbial inoculum to reduce the heavy metal content of the Putian black ducks in claim 1, wherein the microbial animal nutrient solution feed remarkably reduces the mercury, lead and cadmium content of leg muscles of the Putian black ducks.
8. The application of the traditional Chinese medicine microbial inoculum for improving the pig yield according to claim 1, characterized in that the average daily gain and average daily feed intake of the pig are remarkably increased, and the feed conversion ratio is remarkably reduced.
9. The use of the traditional Chinese medicine microbial inoculum for improving the carcass quality of pigs as claimed in claim 1, wherein the microbial animal nutrient solution feed significantly increases the slaughtering rate and the meat purity of pigs and significantly reduces the carcass cooling loss rate.
10. The application of the traditional Chinese medicine microbial inoculum to reduce the heavy metal content of pigs according to claim 1, wherein the microbial animal nutrient solution feed remarkably reduces the mercury, lead and cadmium content of hind leg muscles of pigs.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111140517.7A CN113812521A (en) | 2021-09-28 | 2021-09-28 | Traditional Chinese medicine microbial inoculum and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111140517.7A CN113812521A (en) | 2021-09-28 | 2021-09-28 | Traditional Chinese medicine microbial inoculum and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113812521A true CN113812521A (en) | 2021-12-21 |
Family
ID=78921580
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111140517.7A Pending CN113812521A (en) | 2021-09-28 | 2021-09-28 | Traditional Chinese medicine microbial inoculum and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113812521A (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1969657A (en) * | 2005-11-25 | 2007-05-30 | 重庆市畜牧科学研究院 | Probiotic feed additive used for pig |
| CN102356822A (en) * | 2011-09-28 | 2012-02-22 | 蔡廷贵 | Fermented traditional Chinese medicine feed additive for pigs and preparation method thereof |
| CN103947905A (en) * | 2014-05-19 | 2014-07-30 | 湖南农业大学 | Heat-stress-resistant traditional Chinese medicine microbial feed additive specially used for sows, and preparation method of feed additive |
| CN104161206A (en) * | 2013-07-17 | 2014-11-26 | 河南联合英伟饲料有限公司 | Feed additive for increasing egg yield of hens and preparation method thereof |
| CN105724772A (en) * | 2016-02-24 | 2016-07-06 | 济南亿民动物药业有限公司 | Chinese herbal medicine micro-ecological compound feed additive and preparation and use method thereof |
| CN106942484A (en) * | 2017-03-10 | 2017-07-14 | 苏州顶裕生物科技有限公司 | One boar additive for microbe feedstuff and preparation method thereof |
| CN107095075A (en) * | 2017-06-26 | 2017-08-29 | 宁国市仙之居家庭农场 | It is a kind of to improve the Chinese traditional medicine composition feed and preparation method of Putian duck laying rate |
| CN109527260A (en) * | 2018-11-19 | 2019-03-29 | 山东省潍坊市农业科学院 | A kind of compound poultry bioactivity feed additive and preparation method thereof |
| RU2742866C1 (en) * | 2021-01-11 | 2021-02-11 | Общество с ограниченной ответственностью Научно-исследовательский центр "БОНАКА" | Fodder probiotic supplement for cattle |
| CN112544812A (en) * | 2020-11-23 | 2021-03-26 | 陕西石羊农业科技股份有限公司 | Mixed-strain fermentation type Chinese herbal medicine additive for laying hens in later period of laying, and preparation method and application thereof |
| CN112568324A (en) * | 2020-12-29 | 2021-03-30 | 河南森隆动物保健品有限公司 | Composite probiotic fermented traditional Chinese medicine feed additive and preparation method and application thereof |
-
2021
- 2021-09-28 CN CN202111140517.7A patent/CN113812521A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1969657A (en) * | 2005-11-25 | 2007-05-30 | 重庆市畜牧科学研究院 | Probiotic feed additive used for pig |
| CN102356822A (en) * | 2011-09-28 | 2012-02-22 | 蔡廷贵 | Fermented traditional Chinese medicine feed additive for pigs and preparation method thereof |
| CN104161206A (en) * | 2013-07-17 | 2014-11-26 | 河南联合英伟饲料有限公司 | Feed additive for increasing egg yield of hens and preparation method thereof |
| CN103947905A (en) * | 2014-05-19 | 2014-07-30 | 湖南农业大学 | Heat-stress-resistant traditional Chinese medicine microbial feed additive specially used for sows, and preparation method of feed additive |
| CN105724772A (en) * | 2016-02-24 | 2016-07-06 | 济南亿民动物药业有限公司 | Chinese herbal medicine micro-ecological compound feed additive and preparation and use method thereof |
| CN106942484A (en) * | 2017-03-10 | 2017-07-14 | 苏州顶裕生物科技有限公司 | One boar additive for microbe feedstuff and preparation method thereof |
| CN107095075A (en) * | 2017-06-26 | 2017-08-29 | 宁国市仙之居家庭农场 | It is a kind of to improve the Chinese traditional medicine composition feed and preparation method of Putian duck laying rate |
| CN109527260A (en) * | 2018-11-19 | 2019-03-29 | 山东省潍坊市农业科学院 | A kind of compound poultry bioactivity feed additive and preparation method thereof |
| CN112544812A (en) * | 2020-11-23 | 2021-03-26 | 陕西石羊农业科技股份有限公司 | Mixed-strain fermentation type Chinese herbal medicine additive for laying hens in later period of laying, and preparation method and application thereof |
| CN112568324A (en) * | 2020-12-29 | 2021-03-30 | 河南森隆动物保健品有限公司 | Composite probiotic fermented traditional Chinese medicine feed additive and preparation method and application thereof |
| RU2742866C1 (en) * | 2021-01-11 | 2021-02-11 | Общество с ограниченной ответственностью Научно-исследовательский центр "БОНАКА" | Fodder probiotic supplement for cattle |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104171434B (en) | The pig biology feed additive of develop immunitypty and raising efficiency of feed utilization | |
| CN106260504B (en) | Method for producing microbial fermentation wet feed by using beer yeast paste | |
| WO2017219498A1 (en) | Fermented soybean meal and preparation method therefor | |
| CN103416629B (en) | Pig feed additive capable of effectively improving pig immunity and promoting pig growth | |
| CN102232475B (en) | Physiological regulator for male animals and preparation method thereof | |
| CN103598483B (en) | A kind of reduce piglet stress additive for microbe feedstuff and preparation method thereof | |
| CN102823722A (en) | Preparation method of probiotic fermented feed | |
| CN107853478B (en) | Enzyme-containing Chinese herbal medicine fermented feed additive for reducing methane emission of cattle | |
| CN113812528A (en) | Plant extract additive for improving intestinal health of livestock and poultry | |
| CN111011643A (en) | Bacterium enzyme fermented traditional Chinese medicine for improving growth performance of broiler chicken and preparation method thereof | |
| CN104996841A (en) | Pig starter feed | |
| CN109805172A (en) | A kind of composite bacteria fermentation of Chinese herbal medicine feed and preparation method thereof | |
| CN100527986C (en) | Compound feedstuff additive superfines of Chinese traditional medicine, and preparation method | |
| CN111728098A (en) | Composite probiotics for natural plant fermentation, feed additive for broiler chickens and preparation method thereof | |
| CN104082528B (en) | A kind of feed addictive and preparation method thereof | |
| CN106359849B (en) | A kind of fermented feed of the microelement containing humic acid chelating and preparation method thereof | |
| CN110959748A (en) | Liquid fermented feed prepared from waste vegetables and preparation method thereof | |
| CN107006677A (en) | A kind of feed and its application rich in probiotics and active peptide | |
| CN107095011A (en) | A kind of pigling immunity that strengthens improves the feed addictive of alimentary canal function | |
| CN105410412A (en) | Green high-efficiency pig Chinese herbal medicine feed additive | |
| CN105394390A (en) | Napiergrass feed for feeding lactating sows | |
| CN112006176A (en) | Compound Chinese herbal medicine probiotic fermented feed additive for pigs | |
| CN111631304A (en) | Traditional Chinese medicine residue feed additive and preparation method and application thereof | |
| CN113317270B (en) | Feeding management method for improving production performance of ternary sows | |
| CN107041456B (en) | Fermented bean dreg feed additive and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211221 |
|
| RJ01 | Rejection of invention patent application after publication |