CN109527260A - A kind of compound poultry bioactivity feed additive and preparation method thereof - Google Patents
A kind of compound poultry bioactivity feed additive and preparation method thereof Download PDFInfo
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Abstract
The invention proposes a kind of compound poultry bioactivity feed additives and preparation method thereof, including probiotic composition, herbal component, nutrition composition.Probiotic composition includes bacillus subtilis, bacillus natto and bacillus licheniformis;Herbal component includes STEVIA REBAUDIANA, Radix Codonopsis, Rehmannia glutinosa, Prunella vulgaris, Chinese actinidia root, ramulus cinnamomi, Radix Notoginseng, Chinese yam, Rhizoma Atractylodis Macrocephalae, gynostemma pentaphylla, folium cortex eucommiae, mulberry leaf, radix glycyrrhizae preparata, Poria cocos, dried orange peel, the tuber of pinellia, Radix Astragali, ginger, jujube;Nutrition composition mainly includes mushroom, alfalfa and the mixture of sudangrass.The present invention can not only increase the nutrition content in feed;The also immunity of organisms of deep layer adjusting birds, optimizes archenteric flora ratio, improves antiviral ability;The price of deed of birds can be improved, feedstuff-egg ratio is improved up to 20%, and the death rate reduces about 10%, and laying rate improves 12%, and the hangh unit of birds, beasts and eggs significantly improves, and pouity dwelling place environment also has larger improvement, significantly improves economic benefit of aquaculture.
Description
Technical field
The present invention relates to field of feed additive technology, and in particular to a kind of compound poultry bioactivity feed additive
And preparation method thereof.
Background technique
Feed addictive improves breeding performonce fo animals to basal feed nutritive value is strengthened, and guarantees animal health, saves and raises
Expect cost, improves animal products quality etc. and have obvious effects on.Composite bioactivity feed addition proposed by the present invention
Agent has the double effects of Chinese medicine additive for microbe feedstuff and nutrition type feed additive.Both livestock and poultry autoimmunity can be improved
Ability adjusts microecological balance in animal body, reduces the generation of viral disease, avoid the abuse of antibiotic, and can be enhanced
Nutrition improves the purpose of food safety to reach raising culture efficiency.
The alimentary canal of birds is relatively short, and gut muco-membranous barrier is fragile.Compared with the isometric alimentary canal animal of pig, cattle and sheep more
It is easy the infringement by pathogenic microorganisms.Under the conditions of intensive culture, using feed addictive be prevention and treatment birds intestines problem,
Improve one of the effective means of birds production performance.The speed of growth of poultry is fast, intensive culture production in it is affected by environment compared with
Greatly, intestinal flora balance can be improved using feed addictive, reduces the incidence and the death rate of intestines problem, raising is delivered for sale
Weight reduces feedstuff-meat ratio, and can reduce the harmful gas emissions such as ammonia in henhouse, hydrogen sulfide, improves product quality.It is supported in egg fowl
Grow it is middle using feed addictive, in addition to can disease preventing and treating, improve production performance and the price of deed other than, also have to Egg Quality and change
Kind effect.
Summary of the invention
For the intestines problem for preventing and treating birds, improve the production performance of birds, the present invention provides a kind of compound poultry biology
Activated feed additive and preparation method thereof makes up the deficiency of existing feed additive technology.
A kind of technical solution of the invention is achieved in that a kind of compound poultry bioactivity feed additive, packet
It includes: nutrition composition, herbal component and probiotic composition;
The nutrition composition includes: mushroom, alfalfa and sudangrass;
The herbal component includes: STEVIA REBAUDIANA, Radix Codonopsis, Rehmannia glutinosa, Prunella vulgaris, Chinese actinidia root, ramulus cinnamomi, Radix Notoginseng, Chinese yam, white
Art, gynostemma pentaphylla, folium cortex eucommiae, mulberry leaf, radix glycyrrhizae preparata, Poria cocos, dried orange peel, the tuber of pinellia, Radix Astragali, ginger, jujube;
The probiotic composition includes: bacillus subtilis, bacillus natto and bacillus licheniformis.
As a kind of perferred technical scheme, in parts by weight;
The nutrition composition includes: 15-25 parts of mushroom, 20-30 parts of alfalfa, 20-30 parts of sudangrass;
The herbal component includes: 0.5-3 parts of STEVIA REBAUDIANA, 5-10 parts of Radix Codonopsis, 4-10 parts of Rehmannia glutinosa, Prunella vulgaris 4-10
Part, 8-12 parts of Chinese actinidia root, 8-12 parts of ramulus cinnamomi, 5-9 parts of Radix Notoginseng, 5-10 parts of Chinese yam, 5-10 parts of Rhizoma Atractylodis Macrocephalae, 4-8 parts of gynostemma pentaphylla, folium cortex eucommiae
5-10 parts of 4-8 parts, 4-8 parts of mulberry leaf, 4-8 parts of radix glycyrrhizae preparata, 5-10 parts of Poria cocos, 2.5-5 parts of dried orange peel, 2.5-5 parts of the tuber of pinellia and Radix Astragali, life
2-4 parts of ginger, 3-6 parts of jujube;
The probiotic composition is inoculum concentration 2-5wt.%, bacillus subtilis, bacillus natto, bacillus licheniformis
Weight ratio is 1-2:1-2:1-1.5.
The ingredient of raw material employed in the present invention and pharmacological action are as follows:
The nutrition composition includes: mushroom, alfalfa and sudangrass;
Mushroom: being rich in vitamin B complex, iron, potassium, provitamin D, also contains lentinan, mannitol, trehalose and bacterium
Sugar, glucose, pentosan, methylpentosan, sylvite and mineral element etc..There is good effect with preventing cold for resisting the disease
Fruit.
Alfalfa: contain 5 kinds of vitamin Bs, vitamin C, vitamin E, 10 kinds of minerals and Protoapigenone, carotenoids
The sour three kinds of distinctive nutrients of plant of element, phenolic.With antioxidation etc..
Sudangrass: containing protein abundant and certain pol, feed entirety palatability can be increased, birds can be promoted to adopt
Food.
The herbal component includes: STEVIA REBAUDIANA, Radix Codonopsis, Rehmannia glutinosa, Prunella vulgaris, Chinese actinidia root, ramulus cinnamomi, Radix Notoginseng, Chinese yam, white
Art, gynostemma pentaphylla, folium cortex eucommiae, mulberry leaf, radix glycyrrhizae preparata, Poria cocos, dried orange peel, the tuber of pinellia, Radix Astragali, ginger, jujube;
STEVIA REBAUDIANA contains flavonoids and its glycosides composition in blade, and has and quench one's thirst, diabetes, reduces hypertension, suppression
Bacterium hemostasis, analgesia, fat-reducing and beauty, nourishing Yin and promoting production of body fluid, help digest with clearing heat and detoxicating function, boost metabolism, strong body
The effect of body.
Radix Codonopsis: it is mild-natured, it is sweet in flavor, slightly sour;Returns spleen, lung channel.Tonifying middle-Jiao and Qi, strengthening spleen and tonifying lung.
Rehmannia glutinosa: property is sweet, tepor.Return liver and kidney channel.Enter blood system, the soft and moist drop of matter.Rehmannia glutinosa have heart tonifying, diuresis, it is hypoglycemic and
The effects of increasing peripheral leukocytes, enhancing immune function.With enriching yin of enriching blood, the effect of beneficial to spirit and marrow.
Prunella vulgaris: cold in nature, acrid flavour, hardship.Return liver warp, gallbladder channel.With decompression, anti-arrhythmia, anti-inflammatory, immunosupress, drop
Blood glucose, antibacterial, antiviral, anti-cell toxic action.
Chinese actinidia root: property is sour and astringent, cool.Cure mainly clearing heat and detoxicating, clearing heat and promoting diuresis, preventing tumor anticancer, dispelling wind and eliminating dampness, diuresis hemostasis,
Removing toxicity for detumescence, indigestion, vomiting, rheumatic arthralgia, treating rheumatic ostealgia, Alimentary Tract Cancer, tumor in digestive tract, large carbuncle boil, rheumatism
The diseases such as ostalgia and jaundice.
Ramulus cinnamomi: it is pungent, sweet, it is warm-natured.Return bladder meridian, the heart channel of Hang-Shaoyin, lung channel.With certain antibacterial, antivirus action;To maincenter system
System has antipyretic-antalgic, calmness and anticonvulsant effect;Have the function of apparent anti-inflammatory;It can make cardiac muscle nutritional blood flow
Increase, also has the effects that antiallergy, anticoagulation.
Radix Notoginseng: warm-natured, sweet in flavor, slight bitter, it is nontoxic, return liver, stomach meridian.With enrich blood, protect cardiovascular and cerebrovascular, liver protection, it is antitumor,
Anti-inflammatory, anti-oxidant and immunoregulation effect can enhance the phagocytosis of macrophage.
Chinese yam: property is sweet, flat, returns spleen, lung, kidney channel.Have the function of tonifying spleen nourishing the stomach, beneficial lung fluid, kidney tonifying arresting seminal emission, cures mainly spleen
Stomach weakness, loss of appetite, taste diarrhea, consumptive disease cough and asthma etc..
Rhizoma Atractylodis Macrocephalae: property hardship, sweet, warm;Returns spleen, stomach meridian.There is the effect of strengthening the spleen and replenishing qi, eliminate dampness and have diuretic effect, hidroschesis, miscarriage prevention.
Gynostemma pentaphylla: cold in nature, bitter, micro-sweet, return lung, spleen, kidney channel.Qi-restoratives, heat-clearing, removing toxic substances.For asthenia, consumptive disease is lost
Essence, leukopenia, hyperlipidemia, virus hepatitis, chronic gastroenteritis, chronic bronchitis etc..
Folium cortex eucommiae: it is warm-natured, pungent, return kidney channel, Liver Channel.Nourishing liver and kidney, strengthening the bones and muscles.For kidney deficiency and liver, have a dizzy spell, waist and knee acid
Bitterly, muscles and bones impotence.
Mulberry leaf: sweet in flavor, bitter, cold in nature, distributed in lung channel, Liver Channel.Have and resists pathogenic microorganism such as staphylococcus aureus, B-mode molten
The effect of hemorrhagic streptococcus, corynebacterium diphtheriae, Escherichia coli and Leptospira.There are also anti-inflammatory, hypoglycemic, lowering blood pressure and blood fat,
The effects of diuresis.
Radix glycyrrhizae preparata: it is warm-natured, it is sweet in flavor.Thoughts of returning home warp, lung channel, stomach meridian, the spleen channel.The primary efficacy of radix glycyrrhizae preparata is and middle emergency profit
Lung, removing toxic substances, coordinating the drug actions of a prescription.It is usually used in the benefit of qi and blood two, weakness of the spleen and the stomach, fatigue and weakness, severe palpilation, knotted pulse.
Poria cocos: it is sweet in flavor, light, it is mild-natured.Return heart, lung, spleen, kidney channel.With diuresis, antibacterial action reduces blood glucose, resists swollen
Tumor effect, increases the cytotoxic effect of macrophage, T lymphocyte, the effect of anticancer and air making-up and spleen enlivening.
Dried orange peel: acrid flavour, hardship, it is warm-natured.Converge to the spleen channel, lung channel.Qi-regulating appetizing, it is eliminating dampness and eliminating phlegm, control the spleen-stomach diseases.Belong to qi-regulating drug.It adjusts
Whole digestive system;There is certain prevention effect to artery sclerosis caused by high fat diet;It relievings asthma eliminating the phlegm;Enhance immune function;It is anti-
It is scorching;Shorten bleeding and clotting time;It can promote the digestive juice secretions such as saliva, gastric juice and eliminate enteral pneumatosis.
The tuber of pinellia: warm-natured, toxic, acrid flavour.Returns spleen, stomach meridian.It is eliminating dampness and eliminating phlegm, stopping nausea and vomiting by lowering the adverse flow of QI, dissolving lump and resolving mass.Wet phlegm and cold drink is controlled,
Vomiting, gastric disorder causing nausea, cough and phlegm, fullness in chest, phlegm and headache, dizziness and sleepless.
Radix Astragali: property is sweet, tepor.It is distributed in lung channel, the spleen channel.With enhancing body's immunity, liver protection, diuresis, anti-aging, resists and answer
Sharp, decompression and wide antibacterial action.
Ginger: property and flavor of peppery and warm has the multiple efficacies such as cold dispelling sweating, preventing phlegm from forming and stopping coughing, stomach function regulating, preventing or arresting vomiting.
Jujube: it is warm-natured, it is sweet in flavor.Tonifying spleen stomach function regulating, nourishing generate fluid adjust battalion to defend, and solve poisoning of drug.Control stomach void deficiency of food, the weak loose stool of spleen, gas
Body fluid deficiency for blood, disharmony between ying and wei, palpitation and severe palpitation.
The probiotic composition includes: bacillus subtilis, bacillus natto and bacillus licheniformis.
Bacillus licheniformis: promoting the growth of normal physiological anaerobic bacteria in enteron aisle, adjusts intestinal bacilli illness, restores intestines
Road function;There is special efficacy to enterobacterial infection.To poultry effect than more significant, formula bacillus subtilis using effect is more
It is good.
Bacillus subtilis: having the high-molecular compounds abilities such as brilliant decomposition and inversion protein, inhibits harmful intestinal tract
Bacterium and removal stench, secrete chitin and physiological activator, remove screwworm, inhibit disease.Make simultaneously yellowish pink fresh
Bright, inosinicacid deposition increases, meat taste is fine and smooth, chewy.
Bafillus natto: the quantity and activity of macrophage, enhancing immunity of organism activity can be improved.In addition can also enhance
The phagocytic activity of phagocyte.Studies have found that bafillus natto can significantly increase broiler chicken mucosa-immune function, enhance disease-resistant
Power.The stench of excreta can be eliminated, and then improves integrated environment, to reduce the generation of respiratory tract and other diseases.It can be effective
Anti-nutritional factors in removal or reduction dregs of beans.
Another technical solution of the invention is achieved in that a kind of compound poultry bioactivity feed additive
Preparation method, comprising the following steps:
Step 1, in parts by weight, 0.5-3 parts of STEVIA REBAUDIANA, 5-10 parts of Radix Codonopsis, 4-10 parts of Rehmannia glutinosa, Prunella vulgaris are successively weighed
4-10 parts, 8-12 parts of Chinese actinidia root, 8-12 parts of ramulus cinnamomi, 5-9 parts of Radix Notoginseng, 5-10 parts of Chinese yam, 5-10 parts of Rhizoma Atractylodis Macrocephalae, 4-8 parts of gynostemma pentaphylla, Du
4-8 parts of Zhong Ye, 4-8 parts of mulberry leaf, 4-8 parts of radix glycyrrhizae preparata, 5-10 parts of Poria cocos, 2.5-5 parts of dried orange peel, 2.5-5 parts of the tuber of pinellia and Radix Astragali 5-10
Part, 2-4 parts of ginger, 3-6 parts of jujube, are dissolved in 35-50 parts of water, impregnate 1.5h, decoct be mixed to get decoction mixed liquor twice;
Step 2, in parts by weight, 15-25 parts of mushroom are weighed respectively, 20-30 parts of alfalfa, 20-30 parts of sudangrass, 70
DEG C dry 6h, is processed into 1cm segment, 70 DEG C of dry 6h, obtained sample are pulverized again, are sieved, are crushed to 400
Mesh;
Step 3, step 1 and step 2 are mixed to get mixed liquor, 1wt.% salt is added and obtains culture medium, adjusts pH value
To 6.0-7.0;
Step 4, mixing probiotics is seeded in the culture medium of step 3 by inoculum concentration 2-5wt.%, bacillus subtilis,
Bacillus natto, bacillus licheniformis weight ratio be 1-2:1-2:1-1.5, seal oscillation and fermentation 60-96h;
Step 5, the fermentation liquid obtained to step 4 is centrifuged, take supernatant be added 20-30wt.% protective agent to get.
As a kind of perferred technical scheme, the protective agent is the mixture of citric acid and sodium alginate, the lemon
The weight ratio of acid and sodium alginate is 1:3.
Using above-mentioned technical proposal, the invention has the benefit that feed addictive provided by the invention includes prebiotic
Bacterium component, herbal component and nutrition composition, using bacillus subtilis, bacillus natto and bacillus licheniformis as strain,
Add STEVIA REBAUDIANA, Radix Codonopsis, Rehmannia glutinosa, Prunella vulgaris, Chinese actinidia root, ramulus cinnamomi, Radix Notoginseng, Chinese yam, Rhizoma Atractylodis Macrocephalae, gynostemma pentaphylla, folium cortex eucommiae, mulberry leaf,
Radix glycyrrhizae preparata, Poria cocos, dried orange peel, the tuber of pinellia, Radix Astragali, ginger, the mixture of Chinese herbal medicines of jujube and mushroom, alfalfa, sudangrass
Composite bioactivity feed additive is made not only can increase the content of the nutriment in feed in nutritional blend;Also
Deep layer adjusts the immunity of organisms of birds, optimizes archenteric flora ratio, improves antiviral ability, improves birds productivity
Energy.Preparation method of the present invention is easy to operate, is suitble to high-volume, large-scale production.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Case study on implementation 1:
A kind of preparation method of compound poultry bioactivity feed additive, comprising the following steps:
In parts by weight, 3 parts of STEVIA REBAUDIANA, 10 parts of Radix Codonopsis, 10 parts of Rehmannia glutinosa, 10 parts of Prunella vulgaris, Chinese actinidia root 12 are successively weighed
Part, 12 parts of ramulus cinnamomi, 9 parts of Radix Notoginseng, 10 parts of Chinese yam, 10 parts of Rhizoma Atractylodis Macrocephalae, 8 parts of gynostemma pentaphylla, 8 parts of folium cortex eucommiae, 8 parts of mulberry leaf, 8 parts of radix glycyrrhizae preparata,
10 parts of 10 parts of Poria cocos, 5 parts of dried orange peel, 5 parts of the tuber of pinellia and Radix Astragali, 4 parts of ginger, 6 parts of jujube, are dissolved in 50 parts of water, impregnate 1.5h, twice
Decoction is mixed to get decoction mixed liquor;
Step 2, in parts by weight, 25 parts of mushroom are weighed respectively, 30 parts of alfalfa, 30 parts of sudangrass, 70 DEG C of dry 6h,
It is processed into 1cm segment, 70 DEG C of dry 6h, obtained sample are pulverized again, and sieving is crushed to 400 mesh;
Step 3, step 1 and step 2 are mixed to get mixed liquor, 1wt.% salt is added and obtains culture medium, adjusts pH value
To 7.0;
Step 4, mixing probiotics is seeded in the culture medium of step 3 by inoculum concentration 5wt.%, bacillus subtilis receives
Beans bacillus, bacillus licheniformis weight ratio be 2:2:1.5, seal oscillation and fermentation 96h;
Step 5, the fermentation liquid that step 4 obtains is centrifuged, takes supernatant that 30wt.% protective agent is added, the protective agent is
The weight ratio of the mixture of citric acid and sodium alginate, the citric acid and sodium alginate is 1:3.To obtain the final product.
Composite bioactivity feed additive obtained can improve the price of deed, enhance antiviral ability, reduce
The death rate improves production performance.
In Shandong Changle, laying duck farm carries out feeding experiment, randomly selects 500 laying ducks of No. 8 canopies, weight exists
2800g-3000g.The laying duck group of No. 8 canopies is primarily present that death and culling rate is higher, Egg Quality is low, the low three major issues of the price of deed.Add
Add the composite bioactivity feed additive of the embodiment of the present invention 1,500 tests are sprayed to uniformly with 0.8% additive amount
In the daily daily ration of laying duck, fed according to normal feed mode, it is continuous feed 8 after, the price of deed is improved by 3.89 to 3.56,
The protein concentration of duck's egg obviously improves than control group, and laying rate rises 8%, and death and culling rate has dropped 5%.Continue to feed 8, raise
Material remuneration is improved to 3.44, is tested the albumen absolutely not dispersion phenomenon of duck's egg, is improved the hangh unit of duck's egg, laying rate rises
12%, death and culling rate has dropped 10%, and environment of chicken house has obtained good improvement.
Case study on implementation 2:
A kind of preparation method of compound poultry bioactivity feed additive, comprising the following steps:
In parts by weight, successively weigh 2 parts of STEVIA REBAUDIANA, 8 parts of Radix Codonopsis, 6 parts of Rehmannia glutinosa, 6 parts of Prunella vulgaris, 10 parts of Chinese actinidia root,
10 parts of ramulus cinnamomi, 7 parts of Radix Notoginseng, 7 parts of Chinese yam, 8 parts of Rhizoma Atractylodis Macrocephalae, 6 parts of gynostemma pentaphylla, 6 parts of folium cortex eucommiae, 6 parts of mulberry leaf, 6 parts of radix glycyrrhizae preparata, Poria cocos 8
Part, 4 parts of dried orange peel, 4 parts of the tuber of pinellia and 8 parts of Radix Astragali, 3 parts of ginger, 5 parts of jujube, are dissolved in 45 parts of water, impregnate 1.5h, decoct twice mixed
Conjunction obtains decocting mixed liquor;
Step 2, in parts by weight, 20 parts of mushroom are weighed respectively, 25 parts of alfalfa, 25 parts of sudangrass, 70 DEG C of dry 6h,
It is processed into 1cm segment, 70 DEG C of dry 6h, obtained sample are pulverized again, and sieving is crushed to 400 mesh;
Step 3, step 1 and step 2 are mixed to get mixed liquor, 1wt.% salt is added and obtains culture medium, adjusts pH value
To 6.5;
Step 4, mixing probiotics is seeded in the culture medium of step 3 by inoculum concentration 4wt.%, bacillus subtilis receives
Beans bacillus, bacillus licheniformis weight ratio be 1.5:1.5:1.25, seal oscillation and fermentation 72h;
Step 5, the fermentation liquid that step 4 obtains is centrifuged, takes supernatant that 30wt.% protective agent is added, the protective agent is
The weight ratio of the mixture of citric acid and sodium alginate, the citric acid and sodium alginate is 1:3.To obtain the final product.
Composite bioactivity feed additive obtained can obviously improve disease incident height, death rate height, Egg Quality
The problems such as difference, the price of deed is low, improves body's resistance to disease, improves production performance.
Carry out feeding experiment in Shandong Weifang Fangzi District breeding layer chicken field, randomly selects 400 laying hens of No. 3 canopies, body
Weight is in 1300g-1500g.That there are disease incidents is high by the laying duck group of No. 3 canopies, the death rate is high, Egg Quality is poor, and the price of deed is low
The problems such as.The composite bioactivity feed additive for adding the embodiment of the present invention 2, is uniformly sprayed to 400 with 2% additive amount
It only in the daily daily ration of test laying hen, is fed according to normal feed mode, after continuous nursing 8 days, the state of mind of laying hen is obvious
Improve, the price of deed is improved by 2.43 to 2.12, and laying rate rises 7.8%, and the death rate has dropped about 4%.Continue nursing 8
Day, the hangh unit of egg significantly improves, and the price of deed is improved to 2.01, and the laying rate for testing egg rises 11.8%, extremely
Naughty rate has dropped about 10%.Case study on implementation 3:
A kind of preparation method of compound poultry bioactivity feed additive, comprising the following steps:
In parts by weight, successively weigh 0.5 part of STEVIA REBAUDIANA, 5 parts of Radix Codonopsis, 4 parts of Rehmannia glutinosa, 4 parts of Prunella vulgaris, 8 parts of Chinese actinidia root,
8 parts of ramulus cinnamomi, 5 parts of Radix Notoginseng, 5 parts of Chinese yam, 5 parts of Rhizoma Atractylodis Macrocephalae, 4 parts of gynostemma pentaphylla, 4 parts of folium cortex eucommiae, 4 parts of mulberry leaf, 4 parts of radix glycyrrhizae preparata, Poria cocos 5
Part, 2.5 parts of dried orange peel, 2.5 parts of the tuber of pinellia and 5 parts of Radix Astragali, 2 parts of ginger, 3 parts of jujube, are dissolved in 35 parts of water, impregnate 1.5h, decoct twice
It boils and is mixed to get decoction mixed liquor;
Step 2, in parts by weight, 15 parts of mushroom are weighed respectively, 20 parts of alfalfa, 20 parts of sudangrass, 70 DEG C of dry 6h,
It is processed into 1cm segment, 70 DEG C of dry 6h, obtained sample are pulverized again, and sieving is crushed to 400 mesh;
Step 3, step 1 and step 2 are mixed to get mixed liquor, 1wt.% salt is added and obtains culture medium, adjusts pH value
To 6.0;
Step 4, mixing probiotics is seeded in the culture medium of step 3 by inoculum concentration 4wt.%, bacillus subtilis receives
Beans bacillus, bacillus licheniformis weight ratio be 1:1:1, seal oscillation and fermentation 60h;
Step 5, the fermentation liquid that step 4 obtains is centrifuged, takes supernatant that 20wt.% protective agent is added, the protective agent is
The weight ratio of the mixture of citric acid and sodium alginate, the citric acid and sodium alginate is 1:3.To obtain the final product.
Composite bioactivity feed additive obtained can obviously improve disease incident height, death rate height, Egg Quality
The problems such as difference, the price of deed is low, improves body's resistance to disease, improves production performance.
Carry out feeding experiment in Shandong Weifang Changyi City breeding layer chicken field, randomly selects 300 laying hens of No. 2 canopies, body
Weight is in 1000g-1300g.That there are disease incidents is high by the laying duck group of No. 2 canopies, the death rate is high, Egg Quality is poor, and the price of deed is low
The problems such as.The composite bioactivity feed additive for adding the embodiment of the present invention 3, is uniformly sprayed to 3.5% additive amount
It in the daily daily ration of 300 test laying hens, is fed according to normal feed mode, after continuous nursing 8 days, the state of mind of laying hen is bright
Aobvious to improve, the price of deed is improved by 2.23 to 2.05, and laying rate rises 7.5%, and the death rate has dropped about 3.5%.Continue to feed
It supports 8, the hangh unit of egg significantly improves, and the price of deed is improved to 2.00, and the laying rate for testing egg rises 11.4%,
Death and culling rate has dropped about 10%.
Acute toxicity test
The embodiment of the present invention is poultry fodder additive, therefore selects AA+ broiler chicken as experimental animal.By 60 20 age in days AA
+ broiler chicken is randomly divided into 2 groups, every group 30, weight 1100g or so, carries out acute toxicity test, fasting 12 hours before testing.It will
This feed addictive is administered by 40mL/kg single oral gavage, and control group gives same amount of normal saline, is administered 2 times within one day, dosing interval
It 7 hours, is observed continuously after administration 7 days, records broiler chicken toxic reaction and death toll.The result shows that: compared with the control group, after administration
Broiler chicken has no notable difference, and spirit, behavior, feeding etc. are normal;Also do not occur death.Therefore feed addictive of the invention is anxious
Property toxicity it is low, clinical application safety.
Long term toxicity test
The embodiment of the present invention is poultry fodder additive, therefore selects AA+ broiler chicken as experimental animal.To instruct clinical rational
It uses, carries out spice feeding and evaluate its safety on 16th.
AA+ broiler chicken 100,45~50g of weight, 100 chickens are randomly divided into high, medium and low dosage group and control group, every group
25,20%, 8%, 1% and the feeding of same amount of normal saline spice are given respectively, is fed according to normal feed mode.Continuous 16
Day, the mental status etc. of broiler chicken, 8 days, 16 bus also known as weight are observed and recorded daily, and blood is carried out to broiler chicken in 8 days and 16 days
The detection of liquid biochemical indicator.
Influence of the feed addictive to broiler health situation, mental status.Each group broiler chicken be administered during activity freely, adopt
Food, excretion and other behaviors are without exception, have no toxic reaction.It is discontinued 16 to observe and does not also occur adverse reaction.Illustrate the feeding
Feed additives not will cause obvious adverse effect to broiler chicken.
Influence of the feed addictive to broiler weight.It weighs 2 times during test, it is each to calculate separately administration 8d and drug withdrawal 16d
The average weight of group broiler chicken, body weight increase situation are shown in Table 1.
Influence (unit: g) of 1 composite bioactivity feed additive of table to broiler weight
As can be known from Table 1, each group broiler chicken weight between experimental period has increase, high dose group and middle dose group and control group
Compared to no significant difference, broiler weight, which increases, is slightly below control group or slightly above control group;Low dose group and control group ratio
Relatively there is significant difference (P > 0.05).
Influence of the feed addictive to broiler chicken blood parameters is shown in Table 2.
Influence of 2 composite bioactivity feed additive of table to broiler chicken blood parameters
The test result of each group broiler feeding feed addictive 16d and drug withdrawal 8d as can be seen from Table 2, feed period
Between, the alkaline phosphatase of middle dose group and low dose group significantly increases compared with the control group, it may be possible to physiological facilitation institute
It causes;The detection data of remaining each group is away from there was no significant difference.
By the above toxicity test data, illustrate that the feed addictive is safer, recommends to make in amount ranges in clinic
With being safe and reliable.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, although referring to aforementioned reality
Applying example, invention is explained in detail, for those skilled in the art, still can be to aforementioned each implementation
Technical solution documented by example is modified, or is replaced on an equal basis to part of technical characteristic.It is all in essence of the invention
Within refreshing and principle, made any modification, same replacement, improvement etc. be should all be included in the protection scope of the present invention.
Claims (4)
1. a kind of compound poultry bioactivity feed additive characterized by comprising nutrition composition, herbal component and
Probiotic composition;
The nutrition composition includes: mushroom, alfalfa and sudangrass;
The herbal component include: STEVIA REBAUDIANA, Radix Codonopsis, Rehmannia glutinosa, Prunella vulgaris, Chinese actinidia root, ramulus cinnamomi, Radix Notoginseng, Chinese yam, Rhizoma Atractylodis Macrocephalae,
Gynostemma pentaphylla, folium cortex eucommiae, mulberry leaf, radix glycyrrhizae preparata, Poria cocos, dried orange peel, the tuber of pinellia, Radix Astragali, ginger, jujube;
The probiotic composition includes: bacillus subtilis, bacillus natto and bacillus licheniformis.
2. a kind of compound poultry bioactivity feed additive as claimed in claim 2, which is characterized in that with parts by weight
Meter;
The nutrition composition includes: 15-25 parts of mushroom, 20-30 parts of alfalfa, 20-30 parts of sudangrass;
The herbal component includes: 0.5-3 parts of STEVIA REBAUDIANA, 5-10 parts of Radix Codonopsis, 4-10 parts of Rehmannia glutinosa, 4-10 parts of Prunella vulgaris, rattan
8-12 parts of pears root, 8-12 parts of ramulus cinnamomi, 5-9 parts of Radix Notoginseng, 5-10 parts of Chinese yam, 5-10 parts of Rhizoma Atractylodis Macrocephalae, 4-8 parts of gynostemma pentaphylla, folium cortex eucommiae 4-8
Part, 4-8 parts of mulberry leaf, 4-8 parts of radix glycyrrhizae preparata, 5-10 parts of Poria cocos, 2.5-5 parts of dried orange peel, 2.5-5 parts of the tuber of pinellia and 5-10 parts of Radix Astragali, ginger
2-4 parts, 3-6 parts of jujube;
The probiotic composition is inoculum concentration 2-5wt.%, bacillus subtilis, bacillus natto, bacillus licheniformis weight
Than for 1-2:1-2:1-1.5.
3. a kind of preparation method of compound poultry bioactivity feed additive, which comprises the following steps:
Step 1, in parts by weight, 0.5-3 parts of STEVIA REBAUDIANA, 5-10 parts of Radix Codonopsis, 4-10 parts of Rehmannia glutinosa, Prunella vulgaris 4-10 are successively weighed
Part, 8-12 parts of Chinese actinidia root, 8-12 parts of ramulus cinnamomi, 5-9 parts of Radix Notoginseng, 5-10 parts of Chinese yam, 5-10 parts of Rhizoma Atractylodis Macrocephalae, 4-8 parts of gynostemma pentaphylla, folium cortex eucommiae
5-10 parts of 4-8 parts, 4-8 parts of mulberry leaf, 4-8 parts of radix glycyrrhizae preparata, 5-10 parts of Poria cocos, 2.5-5 parts of dried orange peel, 2.5-5 parts of the tuber of pinellia and Radix Astragali, life
2-4 parts of ginger, 3-6 parts of jujube, are dissolved in 35-50 parts of water, impregnate 1.5h, decoct be mixed to get decoction mixed liquor twice;
Step 2, in parts by weight, 15-25 parts of mushroom are weighed respectively, and 20-30 parts of alfalfa, 20-30 parts of sudangrass, 70 DEG C dry
Dry 6h is processed into 1cm segment, and 70 DEG C of dry 6h, obtained sample are pulverized again, and sieving is crushed to 400 mesh;
Step 3, step 1 and step 2 are mixed to get mixed liquor, 1wt.% salt are added and obtains culture medium, adjust pH value to
6.0-7.0;
Step 4, mixing probiotics is seeded in the culture medium of step 3 by inoculum concentration 2-5wt.%, bacillus subtilis, natto
Bacillus, bacillus licheniformis weight ratio be 1-2:1-2:1-1.5, seal oscillation and fermentation 60-96h;
Step 5, the fermentation liquid obtained to step 4 is centrifuged, take supernatant be added 20-30wt.% protective agent to get.
4. a kind of preparation method of compound poultry bioactivity feed additive as claimed in claim 3, which is characterized in that
The protective agent is the mixture of citric acid and sodium alginate, and the weight ratio of the citric acid and sodium alginate is 1:3.
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