CN104886350A - Chinese herbal medicine microorganism fermentation preparation, as well as preparation method and using method thereof - Google Patents
Chinese herbal medicine microorganism fermentation preparation, as well as preparation method and using method thereof Download PDFInfo
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Abstract
The invention discloses a Chinese herbal medicine microorganism fermentation preparation, as well as a preparation method and using method thereof. The Chinese herbal medicine microorganism fermentation preparation solves the technical problem that when antibiotics are used as medicines or feed additives, defects of having drug residues, having drug-resistant strains, damaging normal flora of animal intestinal tracts, and the like exist. The Chinese herbal medicine microorganism fermentation preparation for beasts and birds is characterized by being prepared from four raw materials: traditional Chinese medicines, culture media, a bacterium solution and an auxiliary material, wherein the traditional Chinese medicines comprise the following components in parts by weight: 5.0-15 parts of astragalus membranaceus, and 1.0-10 parts of angelica sinensis; the culture media comprise the following components in parts by weight: 2.5-17.5 parts of glucose, 2.5-17.5 parts of peptone, 0.35-2.25 parts of yeast powder, 0.15-1.25 parts of dipotassium phosphate, 0.015-0.125 part of magnesium sulfate, 0.0035-0.0225 part of manganese sulfate, 0.8-5.5 parts of calcium carbonate, and 0.45-2.75 parts of sodium chloride; the bacterium solution comprises the following component in parts by volume, 3.0-10 parts of a bacterium solution of enterococcus faecalis; the auxiliary material comprises 5.0-20 parts of bran. The Chinese herbal medicine microorganism fermentation agent disclosed by the invention is green and healthy, has no toxic or side effects, and is safe and effective.
Description
Technical field
The invention belongs to feed addictive prescription design and biological fermentation field, be specifically related to a kind of Chinese herbal medicine microbial fermentation preparation and preparation method thereof.
Background technology
Feeding antibiotic is while bringing huge facilitation to animal productiong, and its drawback is also day by day remarkable, declines and medicine residual etc. as caused endogenous infection and suprainfection, the generation of drug-fast bacteria, immunity of organisms; In addition, antibiotic residue, in animal products, can destroy human gi-tract microecological balance, cause the generation of disease, the health of the infringement mankind, in addition, antibacterial medicine residue, in animal food, also can cause the Long Term Contact of people and medicine, causes the increase of drug-fast bacteria in human body.Therefore, research and develop novel Substitutes For Antibiotic to be very important.At present, the applicating and exploitation of Chinese herbal medicine in animal productiong has become a large study hotspot.
The Chinese herbal medicine resource of China is very abundant, it contains the materials such as rich in protein, carbohydrate, lipid, vitamin, trace element, and active component mainly comprises polysaccharide, alkaloids, organic acid, flavonoids, Polyphenols, glycoside, terpene, volatile oil etc.Chinese herbal medicine plays important effect in livestock and poultry health care, not only can promote growth of animal, improve animal products quality, strengthen animal body immunity, and there is the feature of low toxicity, low-residual, and himself be also nutriment, can be used as the useful supplement of feed Middle nutrition composition, meet animal body to balanced nutritious demand.
Enterococcus faecalis (
enterococcus faecalis) be gram-positive bacteria, this bacterium is classified as enterococcus spp by " the uncle Jie Shi Bacteria Identification handbook " within 1994, published.Enterococcus faecalis is present in the enteron aisle of people and most animals, is the critical bacterial populations in human body intestinal canal, can tolerates hydrochloric acid in gastric juice and bile acid, and enterococcus faecalis, as microorganism formulation, can carry out Tiny ecosystem adjustment to host gastrointestinal tract, strengthens animal immunizing power.
Utilize biofermentation technique to be discharged fast in a large number under the reaction condition of gentleness by the effective active composition in Chinese herbal medicine, produce new active component simultaneously, reduce the toxic and side effect of Chinese herbal medicine.This has played probio and Chinese herbal medicine promotes growth of animal and the double effects strengthening animal body immunity on animal productiong.
Summary of the invention
The technical problem to be solved in the present invention be antibiotic as medicine or feed addictive, there is medicament residue, occur antibody-resistant bacterium, destroy the shortcomings such as animal intestinal normal flora.For solving the problem, the present invention utilizes microbial fermentation technology, ferments to Chinese herbal medicine, provides a kind of Chinese herbal medicine microbial fermentation preparation, preparation method and using method thereof, said preparation green health, has no side effect and safe and effective.
The object of the invention is to realize in the following manner:
A kind of livestock and poultry Chinese herbal medicine microbial fermentation preparation, is characterized in that: be prepared from by following four kinds of raw materials:
One: Chinese medicine material, count with parts by weight: the Radix Astragali 5.0 ~ 15 parts, Radix Angelicae Sinensis 1.0 ~ 10.0 parts;
Two: culture medium, count with parts by weight: glucose 2.5 ~ 10.5 parts, peptone 2.5 ~ 10.5 parts, dusty yeast 0.35 ~ 1.35 part, dipotassium hydrogen phosphate 0.15 ~ 0.75 part, 0.015 ~ 0.075 part, magnesium sulfate, manganese sulfate 0.0035 ~ 0.0135 part, 0.8 ~ 3.3 part, calcium carbonate, 0.45 ~ 1.65 part, sodium chloride;
Three: bacterium liquid, count with volume parts: enterococcus faecalis bacterium liquid 3.0 ~ 10 parts;
Four: auxiliary material, 5.0 ~ 20 parts, wheat bran.
Above-mentioned livestock and poultry Chinese herbal medicine microbial fermentation preparation, is prepared from by following four kinds of raw materials: Chinese medicine material is counted with parts by weight: the Radix Astragali 8.3 parts, Radix Angelicae Sinensis 1.7 parts; Culture medium is counted with parts by weight: glucose 3.0 parts, peptone 3.0 parts, dusty yeast 0.4 part, dipotassium hydrogen phosphate 0.2 part, 0.02 part, magnesium sulfate, manganese sulfate 0.004 part, 1.0 parts, calcium carbonate, 0.5 part, sodium chloride; Bacterium liquid is counted with volume parts: enterococcus faecalis bacterium liquid 5.0 parts; Auxiliary material is counted with parts by weight: 15 parts, wheat bran.
Above-mentioned livestock and poultry Chinese herbal medicine microbial fermentation preparation, described enterococcus faecalis bacterium liquid is obtained by following steps: enterococcus faecalis is first inoculated in slant medium, cultivate 20 ~ 28h under 32 ~ 42 DEG C of conditions to activate, then the suspension that slant medium produces is joined in seed culture medium to expand and cultivate, quiescent culture 18 ~ 20 h under 32 ~ 42 DEG C of conditions is 0.7 × 10 to exponential phase bacterial concentration
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid, i.e. enterococcus faecalis bacterium liquid.
Above-mentioned livestock and poultry Chinese herbal medicine microbial fermentation preparation, described enterococcus faecalis bacterium liquid is obtained by following steps: enterococcus faecalis is first inoculated in slant medium, cultivate 24h under 37 DEG C of conditions to activate, then the suspension that slant medium produces is joined in seed culture medium to expand and cultivate, quiescent culture 18 ~ 20 h under 37 DEG C of conditions is 0.7 × 10 to exponential phase bacterial concentration
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid, i.e. enterococcus faecalis bacterium liquid.
The preparation method of said herbal medicine microbial fermentation preparation, concrete steps are as follows:
(1) take Chinese medicine material, after Chinese medicine material is pulverized, mix, add 100 ~ 500 parts of water, boil 1 ~ 3 h, obtain Chinese medicine feed liquid; Then glucose, peptone, dusty yeast, dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate, calcium carbonate, sodium chloride mixing is added; Under 110 DEG C ~ 120 DEG C conditions after high pressure steam sterilization 15 ~ 20 min, be cooled to 25 ~ 35 DEG C, obtain fermentation medium;
(2) enterococcus faecalis is first inoculated in slant medium, cultivate 20 ~ 28h under 32 ~ 42 DEG C of conditions to activate, then the suspension that slant medium produces is joined in seed culture medium to expand and cultivate, quiescent culture 18 ~ 20 h under 32 ~ 42 DEG C of conditions is 0.7 × 10 to exponential phase bacterial concentration
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid, i.e. enterococcus faecalis bacterium liquid;
(3) fermentation medium after above-mentioned steps (1) sterilizing being lowered the temperature and (2) enterococcus faecalis bacterium liquid 3.0 ~ 10 parts by volume mix, and ferment 2 ~ 5 days, control temperature is at 35 ~ 40 DEG C; After fermentation ends, by centrifugal, zymotic fluid and precipitation are separated;
(4) concentrated for the zymotic fluid in (3) rear low temperature spray drying is obtained dried powder, moisture is not higher than 10%; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, product moisture is not higher than 10%;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.0 × 10
9more than cfu/g.
The preparation method of said herbal medicine microbial fermentation preparation, after high pressure steam sterilization 15 ~ 20 min, is cooled to 25 ~ 35 DEG C, obtains fermentation medium after described step (1) mixes under 115 DEG C of conditions; Described (2) enterococcus faecalis is first inoculated in slant medium, cultivate 24 h under 37 DEG C of conditions to activate, then joined by the suspension that slant medium produces in seed culture medium to expand and cultivate, quiescent culture 18-20 h under 37 DEG C of conditions is 0.7 × 10 to exponential phase bacterial concentration
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid, i.e. enterococcus faecalis bacterium liquid.
The preparation method of said herbal medicine microbial fermentation preparation, described Chinese herbal medicine and wheat bran were through pulverizing 60 ~ 80 mesh sieves.
The using method of said herbal medicine microbial fermentation preparation, be made up of basal diet and the Chinese herbal medicine microbial fermentation preparation comprised according to any one of claim 1-3, the weight proportion of described basal diet and Chinese herbal medicine microbial fermentation preparation is 1:0.4 ~ 1.0.
In the present invention, when weight portion unit is kg, corresponding parts by volume unit is l, and when weight portion unit is g, corresponding parts by volume unit is ml.
In the present invention, the Radix Astragali, taste is sweet warm in nature, containing active ingredients such as astragalin, astragalus polyose, calycosins, can beneficial gas establishing-Yang, nourishing the blood and yin, has inhibitory action to shigella dysenteriae, hemolytic streptococcus, Diplococcus pneumopniae staphylococcus aureus etc., can strengthen animal disease resistant power and immunity; Radix Angelicae Sinensis, taste is sweet warm in nature, containing active ingredients such as Radix Angelicae Sinensis polysaccharide, forulic acid, Ligustilides, have and enrich blood and blood, effect of relaxing bowel, can significantly promote body hematopoiesis function, raise red blood cell, leucocyte and content of hemoglobin, have inhibitory action to shigella dysenteriae, typhoid bacillus, Escherichia coli, hemolytic streptococcus and Diplococcus pneumopniae etc. simultaneously.Glucose, peptone, dusty yeast, dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate, calcium carbonate, sodium chloride, be mainly beneficial bacterium growth and provide necessary nutriment.
Enterococcus faecalis used in the present invention is separated in health pig enteron aisle, has excellent probiotic properties and fermentation character, definite functions, effect is obvious, good stability, belongs to the listed bacterial classification of Ministry of Agriculture's " feed addictive kind catalogue ", can be used safely in livestock and poultry, aquatic livestock.
Relative to prior art, natural reliable, the safety and environmental protection of raw material used in the present invention, by fermenting to compound Chinese herb, effective active composition in accelerated release in vitro Chinese herbal medicine, improve Chinese herbal medicine taste, reduce the toxicity of Chinese herbal medicine, be easier to be absorbed and utilize, played the growth promoting function of probio simultaneously, the adjustment intestines and stomach ecological balance, stimulating animal appetite, promotes growth of animals or poultry, improve food conversion ratio, strengthen animal body immunity antibacterial ability.
Detailed description of the invention
That following is preparation method of the present invention:
When weight portion unit is kg in the present invention, corresponding parts by volume unit is l, and when weight portion unit is g, corresponding parts by volume unit is ml.
A livestock and poultry preparation method for Chinese herbal medicine microbial fermentation preparation, concrete steps are as follows:
(1) preparation of fermentation medium: take Chinese medicine material, count with parts by weight: the Radix Astragali 5.0 ~ 15 parts, Radix Angelicae Sinensis 1.0 ~ 10 parts; After Chinese medicine material is pulverized, mix, add 100 ~ 500 parts of water, boil 1 ~ 3 h, obtain Chinese medicine feed liquid; Then add glucose 2.5 ~ 17.5 parts, peptone 2.5 ~ 17.5 parts, dusty yeast 0.35 ~ 2.25 part, dipotassium hydrogen phosphate 0.15 ~ 1.25 part, 0.015 ~ 0.125 part, magnesium sulfate, manganese sulfate 0.0035 ~ 0.0225 part, 0.8 ~ 5.5 part, calcium carbonate, 0.45 ~ 2.75 part, sodium chloride, mix; Under 110 DEG C ~ 120 DEG C conditions after high pressure steam sterilization 15 ~ 20 min, be cooled to 25 ~ 35 DEG C, obtain fermentation medium;
(2) preparation of enterococcus faecalis bacterium liquid: enterococcus faecalis is first inoculated in slant medium, cultivate 20 ~ 28h under 32 ~ 42 DEG C of conditions to activate, then the suspension that slant medium produces is joined in seed culture medium to expand and cultivate, quiescent culture 18 ~ 20 h under 32 ~ 42 DEG C of conditions is 0.7 × 10 to exponential phase bacterial concentration
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid, i.e. enterococcus faecalis bacterium liquid;
(3) fermentation medium after above-mentioned steps (1) sterilizing being lowered the temperature and (2) enterococcus faecalis bacterium liquid 3.0 ~ 10 parts by volume mix, and ferment 2 ~ 5 days, control temperature is at 35 ~ 40 DEG C; After fermentation ends, by centrifugal, zymotic fluid and precipitation are separated;
(4) concentrated for the zymotic fluid in (3) rear low temperature spray drying is obtained dried powder, moisture is not higher than 10%; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, product moisture is not higher than 10%;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.0 × 10
9more than cfu/g.
Embodiment 1:
A kind of livestock and poultry Chinese herbal medicine microbial fermentation preparation, forms by weight primarily of following raw material: the Radix Astragali 5.0 parts, Radix Angelicae Sinensis 1.0 parts, 8 parts, wheat bran; Bacterium liquid is counted with volume parts: enterococcus faecalis bacterium liquid 3.0 parts.
Livestock and poultry Chinese herbal medicine microbial fermentation preparation preparation method in the present embodiment is:
(1) by said herbal medicine raw material removal of contamination, cross 80 mesh sieves after pulverizing, mix by weight, add 200 parts of water,
Boil 1.5h, then add glucose 6.0 parts, peptone 6.0 parts, dusty yeast 0.8 part, dipotassium hydrogen phosphate 0.4 part, 0.04 part, magnesium sulfate, manganese sulfate 0.008 part, 2.0 parts, calcium carbonate, 1.0 parts, sodium chloride, mix; Under 115 DEG C of conditions after high pressure steam sterilization 15 min, be cooled to 25 DEG C, for subsequent use;
(2) enterococcus faecalis is first inoculated in slant medium, cultivate 24 h under 37 DEG C of conditions to activate, then joined by the suspension that slant medium produces in seed culture medium to expand and cultivate, quiescent culture 20 h under 37 DEG C of conditions, the concentration to exponential phase bacterium liquid is 0.7 × 10
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid;
(3) raw material after above-mentioned steps (1) sterilizing being lowered the temperature and the first order seed bacterium liquid of (2) Liquid Culture mix, and ferment 3 days, control temperature is at 37 DEG C; After fermentation ends, centrifugal by zymotic fluid and precipitation separately;
(4) carry out low temperature spray drying after being concentrated by the zymotic fluid in (3) and obtain dried powder, moisture is 8.6% after testing; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, after testing, product moisture is 9.2%;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.0 × 10
9more than cfu/g.
Embodiment 2:
A kind of livestock and poultry Chinese herbal medicine microbial fermentation preparation, forms by weight primarily of following raw material: the Radix Astragali 7.0 parts, Radix Angelicae Sinensis 3.0 parts, 12 parts, wheat bran; Bacterium liquid is counted with volume parts: enterococcus faecalis bacterium liquid 5.0 parts.
Livestock and poultry Chinese herbal medicine microbial fermentation preparation preparation method in the present embodiment is:
(1) by each herbal raw material removal of contamination, 80 mesh sieves are crossed after pulverizing, mix by weight, add 300 parts of water, boil 2h, then add glucose 9.0 parts, peptone 9.0 parts, dusty yeast 1.2 parts, dipotassium hydrogen phosphate 0.6 part, 0.06 part, magnesium sulfate, manganese sulfate 0.012 part, 3.0 parts, calcium carbonate, 1.5 parts, sodium chloride, mix; Under 115 DEG C of conditions after high pressure steam sterilization 15 min, be cooled to 28 DEG C, for subsequent use; (2) preparation method of described enterococcus faecalis first order seed bacterium liquid is the same.
(3) raw material after above-mentioned steps (1) sterilizing being lowered the temperature and the first order seed bacterium liquid of (2) Liquid Culture mix, and ferment 4 days, control temperature is at 37 DEG C; After fermentation ends, centrifugal by zymotic fluid and precipitation separately;
(4) carry out low temperature spray drying after being concentrated by the zymotic fluid in (3) and obtain dried powder, moisture is 9.0% after testing; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, after testing, product moisture is 9.0%;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.1 × 10
9more than cfu/g.
Embodiment 3:
A kind of livestock and poultry Chinese herbal medicine microbial fermentation preparation, forms by weight primarily of following raw material: the Radix Astragali 8.3 parts, Radix Angelicae Sinensis 1.7 parts, 15 parts, wheat bran; Bacterium liquid is counted with volume parts: enterococcus faecalis bacterium liquid 7.0 parts.
Livestock and poultry Chinese herbal medicine microbial fermentation preparation preparation method in the present embodiment is:
(1) by each herbal raw material removal of contamination, 80 mesh sieves are crossed after pulverizing, mix by weight, add 300 parts of water, boil 2h, then add glucose 9.0 parts, peptone 9.0 parts, dusty yeast 1.2 parts, dipotassium hydrogen phosphate 0.6 part, 0.06 part, magnesium sulfate, manganese sulfate 0.012 part, 3.0 parts, calcium carbonate, 1.5 parts, sodium chloride, mix; Under 115 DEG C of conditions after high pressure steam sterilization 15 min, be cooled to 31 DEG C, for subsequent use; (2) preparation method of described enterococcus faecalis first order seed bacterium liquid is the same.
(3) raw material after above-mentioned steps (1) sterilizing being lowered the temperature and the first order seed bacterium liquid of (2) Liquid Culture mix, and ferment 3 days, control temperature is at 35 DEG C; After fermentation ends, centrifugal by zymotic fluid and precipitation separately;
(4) carry out low temperature spray drying after being concentrated by the zymotic fluid in (3) and obtain dried powder, moisture is 8.9% after testing; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, after testing, product moisture is 8.7%;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.3 × 10
9more than cfu/g.
Embodiment 4:
A kind of livestock and poultry Chinese herbal medicine microbial fermentation preparation, forms by weight primarily of following raw material: the Radix Astragali 10 parts, Radix Angelicae Sinensis 4.5 parts, 5 parts, wheat bran; Bacterium liquid is counted with volume parts: enterococcus faecalis bacterium liquid 10 parts.
Livestock and poultry Chinese herbal medicine microbial fermentation preparation preparation method in the present embodiment is:
(1) by each herbal raw material removal of contamination, 80 mesh sieves are crossed after pulverizing, mix by weight, add 200 parts of water, boil 2.5h, then add glucose 6.0 parts, peptone 6.0 parts, dusty yeast 0.8 part, dipotassium hydrogen phosphate 0.4 part, 0.04 part, magnesium sulfate, manganese sulfate 0.008 part, 2.0 parts, calcium carbonate, 1.0 parts, sodium chloride, mix; Under 115 DEG C of conditions after high pressure steam sterilization 15 min, be cooled to 35 DEG C, for subsequent use; (2) preparation method of described enterococcus faecalis first order seed bacterium liquid is the same.
(3) raw material after above-mentioned steps (1) sterilizing being lowered the temperature and the first order seed bacterium liquid of (2) Liquid Culture mix, and ferment 5 days, control temperature is at 39 DEG C; After fermentation ends, centrifugal by zymotic fluid and precipitation separately;
(4) carry out low temperature spray drying after being concentrated by the zymotic fluid in (3) and obtain dried powder, moisture is 9.3% after testing; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, after testing, product moisture is 9.7%;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.5 × 10
9more than cfu/g.
Embodiment 5:
A kind of livestock and poultry Chinese herbal medicine microbial fermentation preparation, forms by weight primarily of following raw material: the Radix Astragali 8.5 parts, Radix Angelicae Sinensis 3.5 parts, 20 parts, wheat bran; Bacterium liquid is counted with volume parts: enterococcus faecalis bacterium liquid 8.0 parts.
Livestock and poultry Chinese herbal medicine microbial fermentation preparation preparation method in the present embodiment is:
(1) by said herbal medicine raw material removal of contamination, cross 80 mesh sieves after pulverizing, mix by weight, add 400 parts of water,
Boil 1h, then add glucose 12 parts, peptone 12 parts, dusty yeast 1.6 parts, dipotassium hydrogen phosphate 0.8 part, 0.08 part, magnesium sulfate, manganese sulfate 0.016 part, 4.0 parts, calcium carbonate, 2.0 parts, sodium chloride, mix; Under 115 DEG C of conditions after high pressure steam sterilization 15 min, be cooled to 25 DEG C, for subsequent use;
(2) enterococcus faecalis is first inoculated in slant medium, cultivate 24 h under 37 DEG C of conditions to activate, then joined by the suspension that slant medium produces in seed culture medium to expand and cultivate, quiescent culture 20 h under 37 DEG C of conditions, the concentration to exponential phase bacterium liquid is 0.7 × 10
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid;
(3) raw material after above-mentioned steps (1) sterilizing being lowered the temperature and the first order seed bacterium liquid of (2) Liquid Culture mix, and ferment 2 days, control temperature is at 35 DEG C; After fermentation ends, centrifugal by zymotic fluid and precipitation separately;
(4) carry out low temperature spray drying after being concentrated by the zymotic fluid in (3) and obtain dried powder, moisture is 8.6% after testing; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, after testing, product moisture is 8.2%;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.3 × 10
9more than cfu/g.
Embodiment 6:
A kind of livestock and poultry Chinese herbal medicine microbial fermentation preparation, forms by weight primarily of following raw material: the Radix Astragali 9.6 parts, Radix Angelicae Sinensis 3.2 parts, 17 parts, wheat bran; Bacterium liquid is counted with volume parts: enterococcus faecalis bacterium liquid 6.0 parts.
Livestock and poultry Chinese herbal medicine microbial fermentation preparation preparation method in the present embodiment is:
(1) by said herbal medicine raw material removal of contamination, cross 80 mesh sieves after pulverizing, mix by weight, add 350 parts of water,
Boil 3h, then add glucose 10.5 parts, peptone 10.5 parts, dusty yeast 1.4 parts, dipotassium hydrogen phosphate 0.7 part, 0.07 part, magnesium sulfate, manganese sulfate 0.014 part, 3.5 parts, calcium carbonate, 1.75 parts, sodium chloride, mix; Under 115 DEG C of conditions after high pressure steam sterilization 15 min, be cooled to 25 DEG C, for subsequent use;
(2) enterococcus faecalis is first inoculated in slant medium, cultivate 24 h under 37 DEG C of conditions to activate, then joined by the suspension that slant medium produces in seed culture medium to expand and cultivate, quiescent culture 20 h under 37 DEG C of conditions, the concentration to exponential phase bacterium liquid is 0.7 × 10
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid;
(3) raw material after above-mentioned steps (1) sterilizing being lowered the temperature and the first order seed bacterium liquid of (2) Liquid Culture mix, and ferment 3 days, control temperature is at 35 DEG C; After fermentation ends, centrifugal by zymotic fluid and precipitation separately;
(4) carry out low temperature spray drying after being concentrated by the zymotic fluid in (3) and obtain dried powder, moisture is 9.2% after testing; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, after testing, product moisture is 8.6%;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.2 × 10
9more than cfu/g.
Embodiment 7:
A kind of livestock and poultry Chinese herbal medicine microbial fermentation preparation, forms by weight primarily of following raw material: the Radix Astragali 8.0 parts, Radix Angelicae Sinensis 4.0 parts, 10 parts, wheat bran; Bacterium liquid is counted with volume parts: enterococcus faecalis bacterium liquid 4.0 parts.
Livestock and poultry Chinese herbal medicine microbial fermentation preparation preparation method in the present embodiment is:
(1) by said herbal medicine raw material removal of contamination, cross 80 mesh sieves after pulverizing, mix by weight, add 250 parts of water,
Boil 2h, then add glucose 7.5 parts, peptone 7.5 parts, dusty yeast 1.0 parts, dipotassium hydrogen phosphate 0.5 part, 0.05 part, magnesium sulfate, manganese sulfate 0.01 part, 2.5 parts, calcium carbonate, 1.25 parts, sodium chloride, mix; Under 115 DEG C of conditions after high pressure steam sterilization 15 min, be cooled to 27 DEG C, for subsequent use;
(2) enterococcus faecalis is first inoculated in slant medium, cultivate 24 h under 37 DEG C of conditions to activate, then joined by the suspension that slant medium produces in seed culture medium to expand and cultivate, quiescent culture 20 h under 37 DEG C of conditions, the concentration to exponential phase bacterium liquid is 0.7 × 10
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid;
(3) raw material after above-mentioned steps (1) sterilizing being lowered the temperature and the first order seed bacterium liquid of (2) Liquid Culture mix, and ferment 3 days, control temperature is at 37 DEG C; After fermentation ends, centrifugal by zymotic fluid and precipitation separately;
(4) carry out low temperature spray drying after being concentrated by the zymotic fluid in (3) and obtain dried powder, moisture is 9.2% after testing; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, after testing, product moisture is 9.1%;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.0 × 10
9more than cfu/g.
Embodiment 8:
A kind of livestock and poultry Chinese herbal medicine microbial fermentation preparation, forms by weight primarily of following raw material: the Radix Astragali 12.0 parts, Radix Angelicae Sinensis 4.0 parts, 18 parts, wheat bran; Bacterium liquid is counted with volume parts: enterococcus faecalis bacterium liquid 3.0 parts.
Livestock and poultry Chinese herbal medicine microbial fermentation preparation preparation method in the present embodiment is:
(1) by said herbal medicine raw material removal of contamination, cross 80 mesh sieves after pulverizing, mix by weight, add 500 parts of water,
Boil 2.5h, then add glucose 15 parts, peptone 15 parts, dusty yeast 2.0 parts, dipotassium hydrogen phosphate 1.0 parts, 0.1 part, magnesium sulfate, manganese sulfate 0.02 part, 5.0 parts, calcium carbonate, 2.5 parts, sodium chloride, mix; Under 115 DEG C of conditions after high pressure steam sterilization 15 min, be cooled to 27 DEG C, for subsequent use;
(2) enterococcus faecalis is first inoculated in slant medium, cultivate 24 h under 37 DEG C of conditions to activate, then joined by the suspension that slant medium produces in seed culture medium to expand and cultivate, quiescent culture 20 h under 37 DEG C of conditions, the concentration to exponential phase bacterium liquid is 0.7 × 10
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid;
(3) raw material after above-mentioned steps (1) sterilizing being lowered the temperature and the first order seed bacterium liquid of (2) Liquid Culture mix, and ferment 4 days, control temperature is at 37 DEG C; After fermentation ends, centrifugal by zymotic fluid and precipitation separately;
(4) carry out low temperature spray drying after being concentrated by the zymotic fluid in (3) and obtain dried powder, moisture is 9.1% after testing; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, after testing, product moisture is 8.4%;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.0 × 10
9more than cfu/g.
Embodiment 9:
A kind of livestock and poultry Chinese herbal medicine microbial fermentation preparation, forms by weight primarily of following raw material: the Radix Astragali 8.0 parts, Radix Angelicae Sinensis 1 part, 6 parts, wheat bran; Bacterium liquid is counted with volume parts: enterococcus faecalis bacterium liquid 10 parts.
Livestock and poultry Chinese herbal medicine microbial fermentation preparation preparation method in the present embodiment is:
(1) by said herbal medicine raw material removal of contamination, cross 60 mesh sieves after pulverizing, mix by weight, add 100 parts of water,
Boil 1h, then add glucose 2.5 parts, peptone 2.5 parts, dusty yeast 0.35 part, dipotassium hydrogen phosphate 0.15 part, 0.015 part, magnesium sulfate, manganese sulfate 0.0035 part, 0.8 part, calcium carbonate, 0.45 part, sodium chloride, mix; Under 110 DEG C of conditions after high pressure steam sterilization 15min, be cooled to 28 DEG C, for subsequent use;
(2) enterococcus faecalis is first inoculated in slant medium, cultivate 20 h under 33 DEG C of conditions to activate, then joined by the suspension that slant medium produces in seed culture medium to expand and cultivate, quiescent culture 18 h under 32 DEG C of conditions, the concentration to exponential phase bacterium liquid is 0.7 × 10
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid;
(3) raw material after above-mentioned steps (1) sterilizing being lowered the temperature and the first order seed bacterium liquid of (2) Liquid Culture mix, and ferment 2 days, control temperature is at 35 DEG C; After fermentation ends, centrifugal by zymotic fluid and precipitation separately;
(4) carry out low temperature spray drying after being concentrated by the zymotic fluid in (3) and obtain dried powder, moisture is 8.8% after testing; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, after testing, product moisture is 9 %;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.0 × 10
9more than cfu/g.
Embodiment 10:
A kind of livestock and poultry Chinese herbal medicine microbial fermentation preparation, forms by weight primarily of following raw material: the Radix Astragali 8.3 parts, Radix Angelicae Sinensis 1.7 parts, 15 parts, wheat bran; Bacterium liquid is counted with volume parts: enterococcus faecalis bacterium liquid 5 parts.
Livestock and poultry Chinese herbal medicine microbial fermentation preparation preparation method in the present embodiment is:
(1) by said herbal medicine raw material removal of contamination, cross 70 mesh sieves after pulverizing, mix by weight, add 300 parts of water,
Boil 2h, then add glucose 9.0 parts, peptone 9.0 parts, dusty yeast 1.2 parts, dipotassium hydrogen phosphate 0.6 part, 0.06 part, magnesium sulfate, manganese sulfate 0.012 part, 3.0 parts, calcium carbonate, 1.5 parts, sodium chloride, mix; Under 115 DEG C of conditions after high pressure steam sterilization 18min, be cooled to 30 DEG C, for subsequent use;
(2) enterococcus faecalis is first inoculated in slant medium, cultivate 24 h under 35 DEG C of conditions to activate, then joined by the suspension that slant medium produces in seed culture medium to expand and cultivate, quiescent culture 19h under 37 DEG C of conditions, the concentration to exponential phase bacterium liquid is 0.7 × 10
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid;
(3) raw material after above-mentioned steps (1) sterilizing being lowered the temperature and the first order seed bacterium liquid of (2) Liquid Culture mix, and ferment 3 days, control temperature is at 37 DEG C; After fermentation ends, centrifugal by zymotic fluid and precipitation separately;
(4) carry out low temperature spray drying after being concentrated by the zymotic fluid in (3) and obtain dried powder, moisture is 8.9% after testing; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, after testing, product moisture is 9.2%;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.0 × 10
9more than cfu/g.
Embodiment 11:
A kind of livestock and poultry Chinese herbal medicine microbial fermentation preparation, forms by weight primarily of following raw material: the Radix Astragali 15 parts, Radix Angelicae Sinensis 8 parts, 19 parts, wheat bran; Bacterium liquid is counted with volume parts: enterococcus faecalis bacterium liquid 9 parts.
Livestock and poultry Chinese herbal medicine microbial fermentation preparation preparation method in the present embodiment is:
(1) by said herbal medicine raw material removal of contamination, cross 80 mesh sieves after pulverizing, mix by weight, add 500 parts of water,
Boil 3h, then add glucose 17.5 parts, peptone 17.5 parts, dusty yeast 2.25 parts, dipotassium hydrogen phosphate 1.25 parts, 0.125 part, magnesium sulfate, manganese sulfate 0.0225 part, 5.5 parts, calcium carbonate, 2.75 parts, sodium chloride, mix; Under 120 DEG C of conditions after high pressure steam sterilization 20min, be cooled to 20 DEG C, for subsequent use;
(2) enterococcus faecalis is first inoculated in slant medium, cultivate 28h under 35 DEG C of conditions to activate, then joined by the suspension that slant medium produces in seed culture medium to expand and cultivate, quiescent culture 28h under 42 DEG C of conditions, the concentration to exponential phase bacterium liquid is 0.7 × 10
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid;
(3) raw material after above-mentioned steps (1) sterilizing being lowered the temperature and the first order seed bacterium liquid of (2) Liquid Culture mix, and ferment 5 days, control temperature is at 40 DEG C; After fermentation ends, centrifugal by zymotic fluid and precipitation separately;
(4) carry out low temperature spray drying after being concentrated by the zymotic fluid in (3) and obtain dried powder, moisture is 8.7% after testing; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, after testing, product moisture is 8.9%;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.0 × 10
9more than cfu/g.
Embodiment 12:
The using method of Chinese herbal medicine microbial fermentation preparation of the present invention, is made up of basal diet and described Chinese herbal medicine microbial fermentation preparation, and the weight proportion of described basal diet and Chinese herbal medicine microbial fermentation preparation is 1:0.4 ~ 1.0.
Clinical testing
Test example 1: the application of Chinese herbal medicine microbial fermentation preparation in broiler chicken
The Chinese herbal medicine microbial fermentation preparation of this test is product prepared by embodiment 3, and 4000 1 age in days healthy AA Broiler chicks are divided into 4 groups at random, often organizes 1000.Wherein one group is control group, and commercially available Basic drawing of feeding, does not add any additive in daily ration; Test 1 ~ 3 group of Chinese herbal medicine microbial fermentation preparation of feeding in commercially available Basic drawing and embodiment 3 and be respectively 1:0.4,1:0.6,1:1 composition mixed feed according to mass ratio.When 35 age in days, often group randomly draws the close chicken of body weight 8 (often organizing repetition 4), asepticly immediately after sacrificed by exsanguination win complete spleen, thymus gland and the bursa of farbricius, blot surperficial bloodstain, reject after fat and to wet quality with electronic balance weighing, calculate the Immune Organs Index of spleen, thymus gland and the bursa of farbricius.Immune Organs Index (g/kg)=immune organ weight (g)/live body heavy (kg).Weigh in 42 ages in days, ramming material, calculate average daily ingestion amount, average daily gain and feed conversion rate; Record test of full phase chicken death condition.
As shown in Table 1 and Table 2, result of the test shows, the daily gain of test group broiler chicken and daily ingestion amount are all higher than control group broiler chicken, and feedstuff-meat ratio is lower; The broiler chicken death rate of test group is also lower than control group broiler chicken; In Immune Organs Index, the index and spleen index of control group broiler chicken, bursal index and thymus index are all higher than control group broiler chicken.Illustrate that Chinese herbal medicine microbial fermentation preparation can improve Intestine of Broiler microecological balance, improve efficiency of feed utilization, reduce feedstuff-meat ratio, strengthen the resistance against diseases of broiler chicken.
Table 1 Chinese herbal medicine microbial fermentation preparation is on the impact of growth of meat chicken performance
Table 2 Chinese herbal medicine microbial fermentation preparation is on the impact of broiler chicken Immune Organs Index
Test example 2: the application of Chinese herbal medicine microbial fermentation preparation in pig
Average 20 age in days weanling pigs are a collection of on certain pig farm, select close, the healthy piglet of body weight 600, are divided into 4 groups at random.Wherein one group is control group, and daily ration based on commercially available pig starter feed of feeding, does not add any additive in daily ration; Test 1 ~ 3 group of Chinese herbal medicine microbial fermentation preparation of feeding in commercially available basal diet and embodiment 6 and be respectively 1:0.4,1:0.6,1:1 composition compound feed for piglets according to mass ratio.Adopt identical feeding and management condition, feed 21 days, duration of test, observes the hair color of each group, records and add up the indexs such as the body weight of each group, daily ingestion amount, gain in weight, diarrhea rate.
Table 3 Chinese herbal medicine microbial fermentation preparation is on the impact of pig growth performance
as can be seen from table 3 data, test group pig hair color is better than control group, and average daily gain is also higher than control group, and the average feedstuff-meat ratio of test group is 1.27, reduces 11% than control group, and diarrhea rate is also lower than control group.Above-mentioned experiment demonstrates the present invention can improve the price of deed, improves the immunity of pig, reduces the generation of piglet diseases, significantly improves the economic benefit on pig farm.
Claims (8)
1. a livestock and poultry Chinese herbal medicine microbial fermentation preparation, is characterized in that: be prepared from by following four kinds of raw materials:
Chinese medicine material, counts with parts by weight: the Radix Astragali 5.0 ~ 15 parts, Radix Angelicae Sinensis 1.0 ~ 10 parts;
Culture medium, counts with parts by weight: glucose 2.5 ~ 17.5 parts, peptone 2.5 ~ 17.5 parts, dusty yeast 0.35 ~ 2.25 part, dipotassium hydrogen phosphate 0.15 ~ 1.25 part, 0.015 ~ 0.125 part, magnesium sulfate, manganese sulfate 0.0035 ~ 0.0225 part, 0.8 ~ 5.5 part, calcium carbonate, 0.45 ~ 2.75 part, sodium chloride;
Bacterium liquid, counts with volume parts: enterococcus faecalis bacterium liquid 3.0 ~ 10 parts;
Auxiliary material, counts with parts by weight: 5.0 ~ 20 parts, wheat bran.
2. livestock and poultry according to claim 1 Chinese herbal medicine microbial fermentation preparation, is characterized in that: be prepared from by following four kinds of raw materials: Chinese medicine material is counted with parts by weight: the Radix Astragali 8.3 parts, Radix Angelicae Sinensis 1.7 parts; Culture medium is counted with parts by weight: glucose 3.0 parts, peptone 3.0 parts, dusty yeast 0.4 part, dipotassium hydrogen phosphate 0.2 part, 0.02 part, magnesium sulfate, manganese sulfate 0.004 part, 1.0 parts, calcium carbonate, 0.5 part, sodium chloride, and bacterium liquid is counted with volume parts: enterococcus faecalis bacterium liquid 5.0 parts; Auxiliary material, counts with parts by weight: 15 parts, wheat bran.
3. livestock and poultry according to claim 1 Chinese herbal medicine microbial fermentation preparation, it is characterized in that: described enterococcus faecalis bacterium liquid is obtained by following steps: enterococcus faecalis is first inoculated in slant medium, cultivate 20 ~ 28h under 32 ~ 42 DEG C of conditions to activate, then the suspension that slant medium produces is joined in seed culture medium to expand and cultivate, quiescent culture 18 ~ 20 h under 32 ~ 42 DEG C of conditions is 0.7 × 10 to exponential phase bacterial concentration
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid, i.e. enterococcus faecalis bacterium liquid.
4. livestock and poultry according to claim 1 Chinese herbal medicine microbial fermentation preparation, it is characterized in that: described enterococcus faecalis bacterium liquid is obtained by following steps: enterococcus faecalis is first inoculated in slant medium, cultivate 24h under 37 DEG C of conditions to activate, then the suspension that slant medium produces is joined in seed culture medium to expand and cultivate, quiescent culture 18 ~ 20 h under 37 DEG C of conditions is 0.7 × 10 to exponential phase bacterial concentration
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid, i.e. enterococcus faecalis bacterium liquid.
5. the preparation method of the Chinese herbal medicine microbial fermentation preparation according to any one of claim 1-4, is characterized in that: concrete steps are as follows:
(1) take Chinese medicine material, after Chinese medicine material is pulverized, mix, add 100-500 part water, boil 1 ~ 3 h, then add glucose, peptone, dusty yeast, dipotassium hydrogen phosphate, magnesium sulfate, manganese sulfate, calcium carbonate, sodium chloride mixing; Under 110 DEG C ~ 120 DEG C conditions after high pressure steam sterilization 15 ~ 20 min, be cooled to 25 ~ 35 DEG C, obtain fermentation medium;
(2) enterococcus faecalis is first inoculated in slant medium, cultivate 20 ~ 28h under 32 ~ 42 DEG C of conditions to activate, then the suspension that slant medium produces is joined in seed culture medium to expand and cultivate, quiescent culture 18 ~ 20 h under 32 ~ 42 DEG C of conditions is 0.7 × 10 to exponential phase bacterial concentration
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid, i.e. enterococcus faecalis bacterium liquid;
(3) fermentation medium after above-mentioned steps (1) sterilizing being lowered the temperature and (2) enterococcus faecalis bacterium liquid 3.0 ~ 10 parts by volume mix, and ferment 2 ~ 5 days, control temperature is at 35 ~ 40 DEG C; After fermentation ends, by centrifugal, zymotic fluid and precipitation are separated;
(4) concentrated for the zymotic fluid in (3) rear low temperature spray drying is obtained dried powder, moisture is not higher than 10%; Precipitation in (3) mixed with wheat bran, obtain the desciccate containing enterococcus faecalis bacterium powder and zymotic fluid filter residue, product moisture is not higher than 10%;
(5) dried powder in (4) and desciccate are mixed, encapsulation, its viable count is 2.0 × 10
9more than cfu/g.
6. the preparation method of Chinese herbal medicine microbial fermentation preparation according to claim 5, is characterized in that: what described step (1) mixed after high pressure steam sterilization 15 ~ 20 min, is cooled to 25 ~ 35 DEG C, obtains fermentation medium under 115 DEG C of conditions; Described (2) enterococcus faecalis is first inoculated in slant medium, cultivate 24 h under 37 DEG C of conditions to activate, then joined by the suspension that slant medium produces in seed culture medium to expand and cultivate, quiescent culture 18 ~ 20 h under 37 DEG C of conditions is 0.7 × 10 to exponential phase bacterial concentration
8~ 2.0 × 10
8during cfu/ml, prepare first order seed bacterium liquid, i.e. enterococcus faecalis bacterium liquid.
7. the preparation method of Chinese herbal medicine microbial fermentation preparation according to claim 6, is characterized in that: described Chinese herbal medicine and wheat bran were through pulverizing 60-80 mesh sieve.
8. the using method of Chinese herbal medicine microbial fermentation preparation according to claim 1, it is characterized in that: be made up of basal diet and described Chinese herbal medicine microbial fermentation preparation, the weight proportion of described basal diet and Chinese herbal medicine microbial fermentation preparation is 1:0.4 ~ 1.0.
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