CN104982665A - Preparation method of probiotic fermentation cattle feed additive - Google Patents
Preparation method of probiotic fermentation cattle feed additive Download PDFInfo
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Abstract
The invention discloses a preparation method of a probiotic fermentation cattle feed additive. The method comprises the following steps: 1, pre-processing; 2, taking 20-40 parts of mulberry leaf, 5-10 parts of Sorghum sudanense, 10-20 parts of Eichhornia crassipes, 5-15 parts of hawthorn fruit, 10-30 parts of purslane, 10-15 parts of licorice root, 5-10 parts of sweet clover, 5-10 parts of Rheum officinale and 10-15 parts of Radix Platycodonis, mixing to obtain a mixture, adding water having same amount with the mixture, and carrying out pulp refining; 3, adding Bacillus subtilis and Aspergillus niger to the obtained pulp, and carrying out sealed fermentation to obtain a fermentation stock solution; 4, inoculating a Lactobacillus acidophilus nutrient solution into the fermentation stock solution, and fermenting to obtain a primary fermentation solution; and 5, adding sodium chloride to the primary fermentation solution, carrying out standing fermentation to obtain a secondary fermentation solution, filtering, and recovering and preserving the above obtained filtrate. The filtrate can be used in cattle feeds as an additive in order to improve the palatability; and the additive can enhance the colonization ability of beneficial bacteria in the intestinal tract, promotes digestion and absorption, enhances the immunity, improves the disease resistance of bodies, and reduces the incidence of diseases.
Description
Technical field
The invention belongs to field of feed additive technology, be specifically related to a kind of preparation method of ox feed additive of probiotics fermention.
Background technology
Along with the raising of expanding economy and people's living standard, people focus on dietetic nutrition and safety more, along with the development of Tourism in China, greatly promote the development of catering industry, equally, the demand of beef is day by day increased, and ox is in the process of feeding, feed plays vital effect, concerns the growth cycle of ox, beef items quality and feeding cost.In recent years, more problem has been there is in the raising of ox, such as, because feeding and management is improper, the each side factors such as daily ration collocation is unbalanced cause Beef Cattle Growth speed slow, Quality Beef is not good enough, milk production of cow is on the low side, the phenomenons such as disease incidence increases, for this kind of problem, market generally use antibiotic as feed addictive, although achieve good effect, but the thing followed is medicament residue problem, the resistance problems of pathogen, environmental problem, livestock and poultry alimentary tract normal flora imbalance, immunologic function of livestock and birds decline problem, serious medicament residue is produced in ox body, grievous injury ox and the mankind's is healthy.Along with antibiotics additive uses the problems brought to be seen in report, market starts occur comparatively safe Chinese herbal feed additive, some Chinese herbal medicine price costly, aquaculture cost is caused to rise, in addition, containing residues of pesticides and indefinite toxic and side effect in some Chinese herbal medicines, cause the reduction of beef quality, larger threat is formed to food security simultaneously.
Probio is the active microorganism that a class is useful to host, is to be colonizated in human body intestinal canal, reproductive system, can produce definite health efficacy thus improve host's microecological balance, plays the active beneficial microorganism general name of beneficial effect.Bacterium useful in human body, animal body or fungi mainly contain: clostridium butyricum, lactic acid bacteria, Bifidobacterium, lactobacillus acidophilus, actinomyces, saccharomycete etc.The composite reactive probio of the most powerful product of the function studied in the world at present mainly above each quasi-microorganism composition, it is widely used in bioengineering, industrial or agricultural, food security and life and health field.Therefore a kind of feed addictive of probiotics fermention is studied to replace existing antibiotic and pure Chinese medicine composition additive, very necessary to livestock and poultry cultivation.
Summary of the invention
For problems of the prior art, the object of the invention is the preparation method at the ox feed additive providing a kind of probiotics fermention.
The present invention is realized by the following technical programs:
The preparation method of the ox feed additive of described a kind of probiotics fermention, is characterized in that this preparation method is made up of following steps:
1), after getting mulberry leaves, sudangrass, water hyacinth, hawthorn, purslane, Radix Glycyrrhizae, daghestan sweet clover, rheum officinale, balloonflower root as raw material, clean, drying in the shade, pulverize, for subsequent use;
2) each raw material of step 1) process of learning from else's experience respectively mixes according to following weight portion: mulberry leaves 20-40 part, sudangrass 5-10 part, water hyacinth 10-20 part, hawthorn 5-15 part, purslane 10-30 part, Radix Glycyrrhizae 10-15 part, daghestan sweet clover 5-10 part, rheum officinale 5-10 part, balloonflower root 10-15 part, obtained compound, the water mill slurry of weight such as to add in compound, obtain slurries;
3) in step 2) add bacillus subtilis and aspergillus niger respectively in the slurries of gained, stir, sealing, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtain fermenation raw liquid;
4) inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 3-7g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, stops fermenting to obtain one time fermentation liquid;
5) in step 4) gained one time fermentation liquid, add 2-6%(w/v) sodium chloride, after fully stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, filtered by secondary fermentation liquid, filtrate is preserved under being recovered in the temperature conditions of 2-10 DEG C.
The preparation method of the ox feed additive of described a kind of probiotics fermention, is characterized in that step 2) in compound each raw material form by following parts by weight: mulberry leaves 25-35 part, sudangrass 6-9 part, water hyacinth 12-18 part, hawthorn 8-12 part, purslane 15-25 part, Radix Glycyrrhizae 10-12 part, daghestan sweet clover 6-9 part, rheum officinale 5-8 part, balloonflower root 9-15 part.
The preparation method of the ox feed additive of described a kind of probiotics fermention, is characterized in that in compound, each raw material forms by following parts by weight: mulberry leaves 30 parts, sudangrass 7 parts, water hyacinth 15 parts, hawthorn 10 parts, purslane 20 parts, 11 parts, Radix Glycyrrhizae, daghestan sweet clover 7 parts, rheum officinale 6 parts, balloonflower root 12 parts.
The preparation method of the ox feed additive of described a kind of probiotics fermention, is characterized in that total interpolation weight of bacillus subtilis and aspergillus niger in step 3) accounts for the 3-5% of slurries gross weight, and the interpolation of bacillus subtilis and aspergillus oryzae is than being 1:2.
The preparation method of the ox feed additive of described a kind of probiotics fermention, is characterized in that the inoculum concentration of lactobacillus acidophilus nutrient solution in step 4) is inoculate 5g in every 100g fermenation raw liquid.
The preparation method of the ox feed additive of described a kind of probiotics fermention, is characterized in that in step 5), the addition of sodium chloride in zymotic fluid is 4%(w/v).
The preparation method of the ox feed additive of described a kind of probiotics fermention, is characterized in that: when feeding, and the amount adding this feed addictive is 1% of ox feed total amount.
Mulberry leaves has the effect of resisting pathogenic microbes, and mulberry leaves active component all has certain inhibitory action to staphylococcus aureus, beta hemolytic streptococcus, corynebacterium diphtheriae and Escherichia coli, has antiinflammatory action, diuresis; Mulberry leaves, after fermentation process, can ensure the absorption of ox to protein, thus milk production of cow is increased, beef cattle growth is faster; After sudangrass and daghestan sweet clover mixed culture fermentation, effectively can improve content and the digestibility of crude protein.Sudangrass repeatedly can cradle Annual forage crop in 1 year, its cauline leaf quality cuts corn than green grass or young crops and Chinese sorghum is soft, good palatability, has the features such as stronger reproducibility, has bad smell when daghestan sweet clover utilizes as fresh grass, palatability is poor, therefore it extensively utilizes and is restricted, and ferments after being mixed with sudangrass by daghestan sweet clover, namely can eliminate daghestan sweet clover bad smell, both fermentation quality and nutritive value can be improved again, improve content and the digestibility of crude protein; Water hyacinth can improve the palatability of its nutritive value and feed after fermentation, has certain food calling effect and sour fragrance; Hawthorn is containing multivitamin, crataegolic acid, tartaric acid, citric acid, malic acid etc., also containing the mineral matter such as flavonoids, lactone, carbohydrate, protein, fat and calcium, phosphorus, iron, contained lipolytic enzyme can promote the digestion of fat food, promote gastric secretion and increase the functions such as stomach endoenzyme element, three short, bristly hair or beards not only can improve its nutritional labeling after fermentation, cooperatively improve the palatability of feed with other compositions; Purslane, mainly containing tea phenol, alkaloid and multivitamin etc., has desinsection removing toxic substances, blood and bacteriostasis is fallen apart in heat-clearing, can treat the infections of digestive canal such as enteritis diarrhea; After the Chinese herbal medicine fermentations such as Radix Glycyrrhizae, rheum officinale, balloonflower root, effectively can improve its active component performance, improve anti-virus ability.
Compared with existing feed addictive, the present invention has the following advantages:
(1) in ox feed, can bad smell be eliminated with the use of, sudangrass and daghestan sweet clover as additive after fermentation, improve the palatability of feed together with hawthorn;
(2) each raw material is after fermentation process, is convenient to the performance of active component pharmacological action in each raw material, and then strengthens the colonization ability of beneficial bacterium in enteron aisle, and promotion is digested and assimilated, the resistance against diseases of develop immunitypty, raising body, the reduction incidence of disease;
(3) between each material effective component, compatibility is reasonable, and effect is complementary, can produce good synergistic function;
(4) raw materials enjoy stable sources, cheap, production technology is simple, has no side effect and bad reaction.
Detailed description of the invention
By following specific embodiment, the invention will be further described.
Embodiment 1
A preparation method for the ox feed additive of probiotics fermention, after getting mulberry leaves, sudangrass, water hyacinth, hawthorn, purslane, Radix Glycyrrhizae, daghestan sweet clover, rheum officinale, balloonflower root as raw material, clean, drying in the shade, pulverizes, for subsequent use; Each raw material that pulverization process of learning from else's experience respectively is crossed mixes according to following weight portion: mulberry leaves 30 parts, sudangrass 7 parts, water hyacinth 15 parts, hawthorn 10 parts, purslane 20 parts, 11 parts, Radix Glycyrrhizae, daghestan sweet clover 7 parts, rheum officinale 6 parts, balloonflower root 12 parts, obtained compound, the water mill slurry of weight such as to add in compound, obtain slurries; In slurries, add the bacillus subtilis and aspergillus niger that account for slurries gross weight 4%, and the interpolation of bacillus subtilis and aspergillus oryzae is than being 1:2, stirs, seals, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtains fermenation raw liquid; Inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 5g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, stops fermenting to obtain one time fermentation liquid; In one time fermentation liquid, add 4%(w/v) sodium chloride, after fully stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, filtered by secondary fermentation liquid, filtrate is preserved under being recovered in the temperature conditions of 2-10 DEG C.When feeding, the amount adding this feed addictive is 1% of ox feed total amount.
Embodiment 2
A preparation method for the ox feed additive of probiotics fermention, after getting mulberry leaves, sudangrass, water hyacinth, hawthorn, purslane, Radix Glycyrrhizae, daghestan sweet clover, rheum officinale, balloonflower root as raw material, clean, drying in the shade, pulverizes, for subsequent use; Each raw material that pulverization process of learning from else's experience respectively is crossed mixes according to following weight portion: mulberry leaves 20 parts, sudangrass 10 parts, water hyacinth 10 parts, hawthorn 15 parts, purslane 10 parts, 15 parts, Radix Glycyrrhizae, daghestan sweet clover 5 parts, rheum officinale 10 parts, balloonflower root 10 parts, obtained compound, the water mill slurry of weight such as to add in compound, obtain slurries; In slurries, add the bacillus subtilis and aspergillus niger that account for slurries gross weight 3%, and the interpolation of bacillus subtilis and aspergillus oryzae is than being 1:2, stirs, seals, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtains fermenation raw liquid; Inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 4g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, stops fermenting to obtain one time fermentation liquid; In one time fermentation liquid, add 5%(w/v) sodium chloride, after fully stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, filtered by secondary fermentation liquid, filtrate is preserved under being recovered in the temperature conditions of 2-10 DEG C.When feeding, the amount adding this feed addictive is 1% of ox feed total amount.
Embodiment 3
A preparation method for the ox feed additive of probiotics fermention, after getting mulberry leaves, sudangrass, water hyacinth, hawthorn, purslane, Radix Glycyrrhizae, daghestan sweet clover, rheum officinale, balloonflower root as raw material, clean, drying in the shade, pulverizes, for subsequent use; Each raw material that pulverization process of learning from else's experience respectively is crossed mixes according to following weight portion: mulberry leaves 40 parts, sudangrass 5 parts, water hyacinth 20 parts, hawthorn 5 parts, purslane 30 parts, 10 parts, Radix Glycyrrhizae, daghestan sweet clover 10 parts, rheum officinale 5 parts, balloonflower root 15 parts, obtained compound, the water mill slurry of weight such as to add in compound, obtain slurries; In slurries, add the bacillus subtilis and aspergillus niger that account for slurries gross weight 5%, and the interpolation of bacillus subtilis and aspergillus oryzae is than being 1:2, stirs, seals, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtains fermenation raw liquid; Inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 3g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, stops fermenting to obtain one time fermentation liquid; In one time fermentation liquid, add 6%(w/v) sodium chloride, after fully stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, filtered by secondary fermentation liquid, filtrate is preserved under being recovered in the temperature conditions of 2-10 DEG C.When feeding, the amount adding this feed addictive is 1% of ox feed total amount.
Embodiment 4
A preparation method for the ox feed additive of probiotics fermention, after getting mulberry leaves, sudangrass, water hyacinth, hawthorn, purslane, Radix Glycyrrhizae, daghestan sweet clover, rheum officinale, balloonflower root as raw material, clean, drying in the shade, pulverizes, for subsequent use; Each raw material that pulverization process of learning from else's experience respectively is crossed mixes according to following weight portion: mulberry leaves 25 parts, sudangrass 9 parts, water hyacinth 12 parts, hawthorn 12 parts, purslane 15 parts, 12 parts, Radix Glycyrrhizae, daghestan sweet clover 6 parts, rheum officinale 8 parts, balloonflower root 9 parts, obtained compound, the water mill slurry of weight such as to add in compound, obtain slurries; In slurries, add the bacillus subtilis and aspergillus niger that account for slurries gross weight 3.5%, and the interpolation of bacillus subtilis and aspergillus oryzae is than being 1:2, stirs, seals, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtains fermenation raw liquid; Inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 7g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, stops fermenting to obtain one time fermentation liquid; In one time fermentation liquid, add 3%(w/v) sodium chloride, after fully stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, filtered by secondary fermentation liquid, filtrate is preserved under being recovered in the temperature conditions of 2-10 DEG C.When feeding, the amount adding this feed addictive is 1% of ox feed total amount.
Embodiment 5
A preparation method for the ox feed additive of probiotics fermention, after getting mulberry leaves, sudangrass, water hyacinth, hawthorn, purslane, Radix Glycyrrhizae, daghestan sweet clover, rheum officinale, balloonflower root as raw material, clean, drying in the shade, pulverizes, for subsequent use; Each raw material that pulverization process of learning from else's experience respectively is crossed mixes according to following weight portion: mulberry leaves 35 parts, sudangrass 6 parts, water hyacinth 18 parts, hawthorn 8 parts, purslane 25 parts, 10 parts, Radix Glycyrrhizae, daghestan sweet clover 9 parts, rheum officinale 5 parts, balloonflower root 15 parts, obtained compound, the water mill slurry of weight such as to add in compound, obtain slurries; In slurries, add the bacillus subtilis and aspergillus niger that account for slurries gross weight 4.5%, and the interpolation of bacillus subtilis and aspergillus oryzae is than being 1:2, stirs, seals, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtains fermenation raw liquid; Inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 6g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, stops fermenting to obtain one time fermentation liquid; In one time fermentation liquid, add 2%(w/v) sodium chloride, after fully stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, filtered by secondary fermentation liquid, filtrate is preserved under being recovered in the temperature conditions of 2-10 DEG C.When feeding, the amount adding this feed addictive is 1% of ox feed total amount.
Test example 1
Selected for 18 monthly ages, body weight, the male little ox 30 of about 45 jin, is divided into 6 groups at random, often organize 5, be divided into experimental group and control group, experimental group 1 is fed the feed of embodiment 1, experimental group 2 is fed the feed of embodiment 2, and experimental group 3 is fed the feed of embodiment 3, and experimental group 4 is fed the feed of embodiment 4, experimental group 5 is fed the feed of embodiment 5, experimental group 6 is control group, feeds and does not add the conventional feed of additive of the present invention, feeds 60 days, other conditions of feeding are all identical, feed and the results are shown in Table 1:
Table 1
As can be seen from Table 1, adopt feed addictive of the present invention to add in feed, feed to little ox, the indices of experimental group 1-5 is all better than control group, and growth rate is fast, and M & M obviously reduces.
Test example 2
Select the holstein cow 60 that clinical health, lactation period are identical, be divided into 6 groups at random, often organize 10,1-5 group is experimental group, 6th group is control group, and experimental group 1-5 feeds respectively and with the addition of the feed of additive of the present invention, and control group fed does not add additivated feed, feeding method is, control group only provides the conventional feed on basis, after feeding three days, stops doping 12 days, circulate successively, feed altogether 60 days.Experimental group provides the conventional feed that with the addition of additive, and other conditions and environment of feeding are identical, feeding effect part table 2:
Table 2
As can be seen from Table 2, the output of milk of 1-5 group is apparently higher than the output of milk of 6 groups, and total output of milk improves 23.8%, 18.3%, 16.0%, 7.4%, 13.8% respectively, and the incidence of disease of mammitis obviously reduces.
Claims (7)
1. a preparation method for the ox feed additive of probiotics fermention, is characterized in that this preparation method is made up of following steps:
1), after getting mulberry leaves, sudangrass, water hyacinth, hawthorn, purslane, Radix Glycyrrhizae, daghestan sweet clover, rheum officinale, balloonflower root as raw material, clean, drying in the shade, pulverize, for subsequent use;
2) each raw material of step 1) process of learning from else's experience respectively mixes according to following weight portion: mulberry leaves 20-40 part, sudangrass 5-10 part, water hyacinth 10-20 part, hawthorn 5-15 part, purslane 10-30 part, Radix Glycyrrhizae 10-15 part, daghestan sweet clover 5-10 part, rheum officinale 5-10 part, balloonflower root 10-15 part, obtained compound, the water mill slurry of weight such as to add in compound, obtain slurries;
3) in step 2) add bacillus subtilis and aspergillus niger respectively in the slurries of gained, stir, sealing, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtain fermenation raw liquid;
4) inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 3-7g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, stops fermenting to obtain one time fermentation liquid;
5) in step 4) gained one time fermentation liquid, add 2-6%(w/v) sodium chloride, after fully stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, filtered by secondary fermentation liquid, filtrate is preserved under being recovered in the temperature conditions of 2-10 DEG C.
2. the preparation method of the ox feed additive of a kind of probiotics fermention according to right 1, is characterized in that step 2) in compound each raw material form by following parts by weight: mulberry leaves 25-35 part, sudangrass 6-9 part, water hyacinth 12-18 part, hawthorn 8-12 part, purslane 15-25 part, Radix Glycyrrhizae 10-12 part, daghestan sweet clover 6-9 part, rheum officinale 5-8 part, balloonflower root 9-15 part.
3. the preparation method of the ox feed additive of a kind of probiotics fermention according to right 1 or 2, is characterized in that in compound, each raw material forms by following parts by weight: mulberry leaves 30 parts, sudangrass 7 parts, water hyacinth 15 parts, hawthorn 10 parts, purslane 20 parts, 11 parts, Radix Glycyrrhizae, daghestan sweet clover 7 parts, rheum officinale 6 parts, balloonflower root 12 parts.
4. the preparation method of the ox feed additive of a kind of probiotics fermention according to claim 1, it is characterized in that total interpolation weight of bacillus subtilis and aspergillus niger in step 3) accounts for the 3-5% of slurries gross weight, and the interpolation of bacillus subtilis and aspergillus oryzae is than being 1:2.
5. the preparation method of the ox feed additive of a kind of probiotics fermention according to claim 1, is characterized in that the inoculum concentration of lactobacillus acidophilus nutrient solution in step 4) is inoculate 5g in every 100g fermenation raw liquid.
6. the preparation method of the ox feed additive of a kind of probiotics fermention according to claim 1, is characterized in that in step 5), the addition of sodium chloride in zymotic fluid is 4%(w/v).
7. the preparation method of the ox feed additive of a kind of probiotics fermention according to claim 1, is characterized in that: when feeding, and the amount adding this feed addictive is 1% of ox feed total amount.
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| CN107058396A (en) * | 2017-05-31 | 2017-08-18 | 南宁学院 | A kind of preparation method of ox microorganism formulation |
| CN107439808A (en) * | 2017-08-05 | 2017-12-08 | 申亚农牧科技股份有限公司 | A kind of ox Chinese herbal feed additive containing compound probiotic |
| CN107594147A (en) * | 2017-09-28 | 2018-01-19 | 淮北市渭水源农业有限公司 | A kind of feed addictive for improving milk production of cow, improving lactoprotein and preparation method thereof |
| CN108719579A (en) * | 2018-05-16 | 2018-11-02 | 北京秦凰达同生物科技有限公司 | Ferment animal's diet replenishers and preparation method thereof |
| CN109845901A (en) * | 2017-11-30 | 2019-06-07 | 南宁学院 | A kind of fermented type mulberry leaf ox feed and preparation method thereof |
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