CN107058396A - A kind of preparation method of ox microorganism formulation - Google Patents
A kind of preparation method of ox microorganism formulation Download PDFInfo
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Abstract
The invention belongs to microorganism formulation technical field, especially a kind of preparation method of ox microorganism formulation, it is prepared from by preparing the steps such as zymotic fluid, culture, bacterium powder preparation, the microorganism formulation can in ox intestines and stomach amount reproduction, formed dominant microflora, promote beneficial bacterium growth and breeding, germ reproduction in Reverse transcriptase enteron aisle, reduce infringement of the pathogen to intestines and stomach, the materials such as lactic acid, hydrogen peroxide can also be produced in intestines and stomach simultaneously, can effectively suppress the activity of pathogenic bacteria；The microorganism formulation can also improve gastrointestinal function, promote the wriggling of intestines and stomach, improve utilization rate of the ox to feed, promote growing for ox；In addition the immunologic function of ox can also be improved, is built up health, the illness rate of ox is reduced, guarantee is provided for the healthy growth of ox.
Description
Technical field
The invention belongs to microorganism formulation technical field, especially a kind of preparation method of ox microorganism formulation.
Background technology
Beef is a kind of by popular food, and its beef is of high nutritive value, and it contains abundant protein, amino
Sour ratio of components pork needs closer to human body, can improve body resistance against diseases, to growing and Post operation, the people of aftercare
Lost blood and particularly suitable in terms of repair tissue, severe winter food beef in supplement, have warm stomach effect, be severe winter good merchantable brand, the traditional Chinese medical science
Think：Beef has tonifying middle-Jiao and Qi, nourishes taste, strong muscles and bones, breath wind that reduces phlegm, the function of saliva of quenching the thirst, it is adaptable to the sinking of qi of middle-jiao,
The people of body void of breathing hard, soreness of bones and muscles and anaemia prolonged illness and yellow dizzy people in face eats.
At present, most of raiser oxen carry out nutrition arrangement in breeding process to daily ration, substantially meet the growth of ox and need
Ask, but in real process, ox can not reach theoretical effect to the digestibility of feed, cause that production is slow, the rate of animals delivered to the slaughter-house is low；
This all declines to gastrointestinal function, pathogen intrusion and the factor such as irritability it is related；And due to the valency of microorganism formulation
Lattice are high, action effect is poor, therefore its application does not obtain the popularization of large area also.
The content of the invention
It is achieved particular by following technical scheme：
In order to solve the above technical problems, the present invention provides a kind of preparation method of ox microorganism formulation, including following step
Suddenly：
(1) zymotic fluid is prepared：By fowl and animal excrement and mulberry leaves according to (5.5~6.3)：1 weight proportion is mixed, and is placed in and is stirred
Mix in machine and crush, it is 70~80% to add deionized water adjustment moisture, it is 6.8~7.5 to add pH adjusting agent regulation pH value
Obtain zymotic fluid；
(2) cultivate：Microorganism fungus kind is inoculated in the zymotic fluid in step (1), it is 45~50 DEG C to control temperature, ventilation
Condition is 0.3~0.5V/V, and ferment 6~10h, then it is 120~160r/min to add nutrients to be transferred to shaking speed, and temperature is 35
Fermentation is shaken in~45 DEG C, nutrient solution is obtained；
(3) prepared by bacterium powder：By step (2) nutrient solution, 3000~400r/min centrifuges 25~30min under the conditions of 4~6 DEG C,
Dry to powder, vacuum packaging.
The pH adjusting agent is calcium carbonate, sodium carbonate and sodium hydroxide, and its mass ratio is (2~4)：1.5：1.
The microorganism fungus kind includes Paenibacillus polymyxa, actinomyces, saccharomycete and rhizopus.
The Paenibacillus polymyxa, actinomyces, the inoculum concentration of saccharomycete and rhizopus are respectively that step (1) zymotic fluid is total
5~7%, 1~3%, 2~4% and the 2~4% of amount.
The nutrients be by 10~15 parts of corn flour, 3~5 parts of glycerine, 2~4 parts of xylo-oligosaccharide, 1~3 part of glucose,
Through 100~120 DEG C of high-temperature sterilizations after 1~3 part of mixing of potassium dihydrogen phosphate, it is cooled to 35~40 DEG C and is made.
Paenibacillus polymyxa in the nutrient solution, actinomyces, the CFU numbers of saccharomycete and rhizopus be respectively 107/g,
110/g, 108/g and more than 105/g.
Step (1) zymotic fluid is 15~20KHz being inoculated with preposition 10~20s of processing in ultrasonic wave, frequency.
The ox microorganism formulation, additive capacity is the 0.1~1% of ox daily ration.
Beneficial effect of the present invention：
Microorganism formulation of the present invention is prepared from by preparing the steps such as zymotic fluid, culture, bacterium powder preparation, the microorganism system
Agent can be in ox intestines and stomach in amount reproduction, formation dominant microflora, promotion beneficial bacterium growth and breeding, Reverse transcriptase enteron aisle
Germ reproduction, reduces infringement of the pathogen to intestines and stomach, while the materials such as lactic acid, hydrogen peroxide can also be produced in intestines and stomach,
The activity of pathogenic bacteria can effectively be suppressed；The microorganism formulation can also improve gastrointestinal function, promote the wriggling of intestines and stomach, carry
High ox promotes growing for ox to the utilization rate of feed；In addition the immunologic function of ox can also be improved, is built up health, ox is reduced
Illness rate, provide guarantee for the healthy growth of ox.
Embodiment
Tuberculosis specific embodiment is limited technical scheme is further below, but claimed
Scope is not only limited to made description.
Embodiment 1
A kind of preparation method of ox microorganism formulation, comprises the following steps：
(1) zymotic fluid is prepared：By fowl and animal excrement and mulberry leaves according to 5.5：1 weight proportion is mixed, and is placed in mixer
Crush, it is 70% that addition deionized water, which adjusts moisture, add pH adjusting agent regulation pH value and obtain zymotic fluid for 6.8；
(2) cultivate：Microorganism fungus kind is inoculated in the zymotic fluid in step (1), it is 45 DEG C, aeration condition to control temperature
For 0.3V/V, ferment 6h, then it is 120r/min to add nutrients to be transferred to shaking speed, and temperature is concussion fermentation in 35 DEG C, is obtained
Nutrient solution；
(3) prepared by bacterium powder：By step (2) nutrient solution, 3000r/min centrifuges 25min under the conditions of 4 DEG C, dries to powder,
Vacuum packaging.
The pH adjusting agent is calcium carbonate, sodium carbonate and sodium hydroxide, and its mass ratio is 2：1.5：1.
The microorganism fungus kind includes Paenibacillus polymyxa, actinomyces, saccharomycete and rhizopus.
The Paenibacillus polymyxa, actinomyces, the inoculum concentration of saccharomycete and rhizopus are respectively that step (1) zymotic fluid is total
5%, 1%, 2% and the 2% of amount.
The nutrients is by 10 parts of corn flour, 3 parts of glycerine, 2 parts of xylo-oligosaccharide, 1 part of glucose, 1 part of potassium dihydrogen phosphate
Through 100 DEG C of high-temperature sterilizations after mixing, it is cooled to 35 DEG C and is made.
Paenibacillus polymyxa in the nutrient solution, actinomyces, the CFU numbers of saccharomycete and rhizopus be respectively 107/g,
110/g, 108/g and more than 105/g.
Step (1) zymotic fluid be inoculated with it is preposition 10s is handled in ultrasonic wave, frequency is 15KHz.
The ox microorganism formulation, additive capacity is the 0.1% of ox daily ration.
Embodiment 2
A kind of preparation method of ox microorganism formulation, comprises the following steps：
(1) zymotic fluid is prepared：By fowl and animal excrement and mulberry leaves according to 6.3：1 weight proportion is mixed, and is placed in mixer
Crush, it is 80% that addition deionized water, which adjusts moisture, add pH adjusting agent regulation pH value and obtain zymotic fluid for 7.5；
(2) cultivate：Microorganism fungus kind is inoculated in the zymotic fluid in step (1), it is 50 DEG C, aeration condition to control temperature
For 0.5V/V, ferment 10h, then it is 160r/min to add nutrients to be transferred to shaking speed, and temperature is concussion fermentation in 45 DEG C, is obtained
Nutrient solution；
(3) prepared by bacterium powder：By step (2) nutrient solution, 4000r/min centrifuges 30min under the conditions of 6 DEG C, dries to powder,
Vacuum packaging.
The pH adjusting agent is calcium carbonate, sodium carbonate and sodium hydroxide, and its mass ratio is 4：1.5：1.
The microorganism fungus kind includes Paenibacillus polymyxa, actinomyces, saccharomycete and rhizopus.
The Paenibacillus polymyxa, actinomyces, the inoculum concentration of saccharomycete and rhizopus are respectively that step (1) zymotic fluid is total
7%, 3%, 4% and the 4% of amount.
The nutrients is by 15 parts of corn flour, 5 parts of glycerine, 4 parts of xylo-oligosaccharide, 3 parts of glucose, 3 parts of potassium dihydrogen phosphate
Through 120 DEG C of high-temperature sterilizations after mixing, it is cooled to 40 DEG C and is made.
Paenibacillus polymyxa in the nutrient solution, actinomyces, the CFU numbers of saccharomycete and rhizopus be respectively 107/g,
110/g, 108/g and more than 105/g.
Step (1) zymotic fluid be inoculated with it is preposition 20s is handled in ultrasonic wave, frequency is 20KHz.
The ox microorganism formulation, additive capacity is the 1% of ox daily ration.
Embodiment 3
A kind of preparation method of ox microorganism formulation, comprises the following steps：
(1) zymotic fluid is prepared：By fowl and animal excrement and mulberry leaves according to 6.1：1 weight proportion is mixed, and is placed in mixer
Crush, it is 75% that addition deionized water, which adjusts moisture, add pH adjusting agent regulation pH value and obtain zymotic fluid for 7.2；
(2) cultivate：Microorganism fungus kind is inoculated in the zymotic fluid in step (1), it is 48 DEG C, aeration condition to control temperature
For 0.4V/V, ferment 7h, then it is 140r/min to add nutrients to be transferred to shaking speed, and temperature is concussion fermentation in 38 DEG C, is obtained
Nutrient solution；
(3) prepared by bacterium powder：By step (2) nutrient solution, 3500r/min centrifuges 28min under the conditions of 5 DEG C, dries to powder,
Vacuum packaging.
The pH adjusting agent is calcium carbonate, sodium carbonate and sodium hydroxide, and its mass ratio is 3：1.5：1.
The microorganism fungus kind includes Paenibacillus polymyxa, actinomyces, saccharomycete and rhizopus.
The Paenibacillus polymyxa, actinomyces, the inoculum concentration of saccharomycete and rhizopus are respectively that step (1) zymotic fluid is total
6%, 3%, 3% and the 2% of amount.
The nutrients is by 14 parts of corn flour, 4 parts of glycerine, 3 parts of xylo-oligosaccharide, 3 parts of glucose, 2 parts of potassium dihydrogen phosphate
Through 105 DEG C of high-temperature sterilizations after mixing, it is cooled to 40 DEG C and is made.
Paenibacillus polymyxa in the nutrient solution, actinomyces, the CFU numbers of saccharomycete and rhizopus be respectively 107/g,
110/g, 108/g and more than 105/g.
Step (1) zymotic fluid be inoculated with it is preposition 15s is handled in ultrasonic wave, frequency is 18KHz.
The ox microorganism formulation, additive capacity is the 0.8% of ox daily ration.
Test example 4
A kind of preparation method of ox microorganism formulation, comprises the following steps：
(1) zymotic fluid is prepared：By fowl and animal excrement and mulberry leaves according to 5.8：1 weight proportion is mixed, and is placed in mixer
Crush, it is 72% that addition deionized water, which adjusts moisture, add pH adjusting agent regulation pH value and obtain zymotic fluid for 7；
(2) cultivate：Microorganism fungus kind is inoculated in the zymotic fluid in step (1), it is 46 DEG C, aeration condition to control temperature
For 0.5V/V, ferment 9h, then it is 150r/min to add nutrients to be transferred to shaking speed, and temperature is concussion fermentation in 37 DEG C, is obtained
Nutrient solution；
(3) prepared by bacterium powder：By step (2) nutrient solution, 3800r/min centrifuges 26min under the conditions of 5 DEG C, dries to powder,
Vacuum packaging.
The pH adjusting agent is calcium carbonate, sodium carbonate and sodium hydroxide, and its mass ratio is 2：1.5：1.
The microorganism fungus kind includes Paenibacillus polymyxa, actinomyces, saccharomycete and rhizopus.
The Paenibacillus polymyxa, actinomyces, the inoculum concentration of saccharomycete and rhizopus are respectively that step (1) zymotic fluid is total
7%, 1%, 4% and the 2% of amount.
The nutrients is by 15 parts of corn flour, 3 parts of glycerine, 4 parts of xylo-oligosaccharide, 1 part of glucose, 1 part of potassium dihydrogen phosphate
Through 120 DEG C of high-temperature sterilizations after mixing, it is cooled to 40 DEG C and is made.
Paenibacillus polymyxa in the nutrient solution, actinomyces, the CFU numbers of saccharomycete and rhizopus be respectively 107/g,
110/g, 108/g and more than 105/g.
Step (1) zymotic fluid be inoculated with it is preposition 10s is handled in ultrasonic wave, frequency is 20KHz.
The ox microorganism formulation, additive capacity is the 0.7% of ox daily ration.
Embodiment 5
A kind of preparation method of ox microorganism formulation, comprises the following steps：
(1) zymotic fluid is prepared：By fowl and animal excrement and mulberry leaves according to (5.5~6.3)：1 weight proportion is mixed, and is placed in and is stirred
Mix in machine and crush, it is 70~80% to add deionized water adjustment moisture, it is 6.8~7.5 to add pH adjusting agent regulation pH value
Obtain zymotic fluid；
(2) cultivate：Microorganism fungus kind is inoculated in the zymotic fluid in step (1), it is 45~50 DEG C to control temperature, ventilation
Condition is 0.3~0.5V/V, and ferment 6~10h, then it is 120~160r/min to add nutrients to be transferred to shaking speed, and temperature is 35
Fermentation is shaken in~45 DEG C, nutrient solution is obtained；
(3) prepared by bacterium powder：By step (2) nutrient solution, 3000~400r/min centrifuges 25~30min under the conditions of 4~6 DEG C,
Dry to powder, vacuum packaging.
The pH adjusting agent is calcium carbonate, sodium carbonate and sodium hydroxide, and its mass ratio is (2~4)：1.5：1.
The microorganism fungus kind includes Paenibacillus polymyxa, actinomyces, saccharomycete and rhizopus.
The Paenibacillus polymyxa, actinomyces, the inoculum concentration of saccharomycete and rhizopus are respectively that step (1) zymotic fluid is total
5~7%, 1~3%, 2~4% and the 2~4% of amount.
The nutrients be by 10~15 parts of corn flour, 3~5 parts of glycerine, 2~4 parts of xylo-oligosaccharide, 1~3 part of glucose,
Through 100~120 DEG C of high-temperature sterilizations after 1~3 part of mixing of potassium dihydrogen phosphate, it is cooled to 35~40 DEG C and is made.
Paenibacillus polymyxa in the nutrient solution, actinomyces, the CFU numbers of saccharomycete and rhizopus be respectively 107/g,
110/g, 108/g and more than 105/g.
Step (1) zymotic fluid is 15~20KHz being inoculated with preposition 10~20s of processing in ultrasonic wave, frequency.
The ox microorganism formulation, additive capacity is the 0.1~1% of ox daily ration.
Test example 1
The microorganism formulation that wherein embodiment group is prepared using the present invention, control group uses the microorganism system that in the market is sold
Agent, is added into the daily feed of ox, is observed by 90d raising result, as a result as follows：
As seen from the experiment, the microorganism formulation prepared using the present invention program, the increased weight of ox is substantially better than pair
According to group, the conversion ratio of feed is apparently higher than control group, wherein the microorganism formulation best results prepared with the scheme of embodiment 3,90d
The increased weight 63kg of ox, feed conversion rate is 2.57.
Above-mentioned test ox in empty stomach tendon and vessel blood sampling 10mL before 91d mornings, and is placed on rush after 90d raising
In solidifying vacuum tube, then place at room temperature after 20min, serum is centrifuged out, determine IgA, IgM, IgG in serum
Content is as a result as follows：
As seen from the experiment, the immunologic function of ox, wherein blood can be effectively improved using microorganism formulation of the present invention
IgA, IgM in clear, IgG content are all remarkably higher than control group, wherein, IgA content the most obvious with the effect of the scheme of embodiment 4
57.46ng/ml is reached, IgM contents reach that 97.34 μ g/ml, IgA contents reach 437.02 μ g/ml.
Claims (8)
1. a kind of preparation method of ox microorganism formulation, it is characterised in that comprise the following steps：
(1) zymotic fluid is prepared：By fowl and animal excrement and mulberry leaves according to (5.5~6.3)：1 weight proportion is mixed, and is placed in mixer
Middle to crush, addition deionized water adjustment moisture is 70~80%, and adding pH adjusting agent regulation pH value must send out for 6.8~7.5
Zymotic fluid；
(2) cultivate：Microorganism fungus kind is inoculated in the zymotic fluid in step (1), it is 45~50 DEG C, aeration condition to control temperature
For 0.3~0.5V/V, ferment 6~10h, then it is 120~160r/min to add nutrients to be transferred to shaking speed, and temperature is 35~45
Fermentation is shaken in DEG C, nutrient solution is obtained；
(3) prepared by bacterium powder：By step (2) nutrient solution, 3000~400r/min centrifuges 25~30min under the conditions of 4~6 DEG C, dries
To powder, vacuum packaging.
2. the preparation method of ox microorganism formulation as claimed in claim 1, it is characterised in that the pH adjusting agent is carbonic acid
Calcium, sodium carbonate and sodium hydroxide, its mass ratio are (2~4)：1.5：1.
3. the preparation method of ox microorganism formulation as claimed in claim 1, it is characterised in that the microorganism fungus kind includes many
Viscous series bacillus, actinomyces, saccharomycete and rhizopus.
4. the preparation method of ox microorganism formulation as claimed in claim 1, it is characterised in that the Paenibacillus polymyxa,
The inoculum concentration of actinomyces, saccharomycete and rhizopus is respectively 5~7%, 1~3%, 2~4% and the 2 of step (1) zymotic fluid total amount
~4%.
5. the preparation method of ox microorganism formulation as claimed in claim 1, it is characterised in that the nutrients is by corn flour
10~15 parts, 3~5 parts of glycerine, 2~4 parts of xylo-oligosaccharide, 1~3 part of glucose, after the mixing of 1~3 part of potassium dihydrogen phosphate through 100~
120 DEG C of high-temperature sterilizations, are cooled to 35~40 DEG C and are made.
6. the preparation method of ox microorganism formulation as claimed in claim 1, it is characterised in that many viscous class buds in the nutrient solution
Spore bacillus, actinomyces, the CFU numbers of saccharomycete and rhizopus are respectively 107/g, 110/g, 108/g and more than 105/g.
7. the preparation method of ox microorganism formulation as claimed in claim 1, it is characterised in that step (1) zymotic fluid exists
Inoculation is preposition to handle 10~20s in ultrasonic wave, and frequency is 15~20KHz.
8. a kind of ox microorganism formulation, it is characterised in that additive capacity is the 0.1~1% of ox daily ration.
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