CN106666075A - Production technology of magnetic field assisted fermentation feed rich in natural antibacterial agent - Google Patents
Production technology of magnetic field assisted fermentation feed rich in natural antibacterial agent Download PDFInfo
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- CN106666075A CN106666075A CN201611214806.6A CN201611214806A CN106666075A CN 106666075 A CN106666075 A CN 106666075A CN 201611214806 A CN201611214806 A CN 201611214806A CN 106666075 A CN106666075 A CN 106666075A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention discloses a production technology of a magnetic field assisted fermentation feed rich in a natural antibacterial agent. Streptococcus lactis is used as a fermentation strain, raw materials of rapeseed meal, cottonseed meal and soybean meal are mixed to be used as a medium, and a solid state fermentation method is used to produce the fermentation feed rich in the natural antibacterial agent of nisin. The technical technology comprises the following steps: the basic medium is prepared, bacteria are subjected to an expanding culture, and the mixed protein feed solid state magnetic field assisted fermentation is conducted to obtain the finished products. Mixed raw material meals with different contents of proteins, carbohydrates and fats are as follows in parts by weight: 25-40 parts of rapeseed meal, 25-40 parts of the cottonseed meal and 25-50 parts of soybean meal, the materials are mixed, the mixture is inoculated for the fermentation, and the fermentation feed with the antibacterial agent of the nisin is produced by the technology. The fermentation feed is added into compound feed in a certain proportion, improves the palatability and utilization rate of the feed, also can effectively realize no addition of antibiotics, improves the feed conversion rate, and at the same time ensures the safety of the feed.
Description
Technical field
The present invention relates to a kind of production technology of fermented feed, more particularly to a kind of auxiliary of the magnetic field rich in natural antibacterial agent
Fermented feed production technology.
Background technology
Nisin Nisin is a kind of micromolecule polypeptide antibiotic substance or antibacterial produced by some streptococcus acidi lacticis
Element, molecular formula is C143H228N42O37S7, is made up of 34 amino acid residues, and Nisin can suppress most of Gram-positive
Bacterium and its growth and breeding of spore, such as staphylococcus, Streptococcus, lactic acid bar Coccuss and Clostridium and
The antibacterial of bacillus, it is particularly obvious to staphylococcus aureuses, Streptococcus hemolyticuss, bacillus botulinus effect, can be effectively
Extend the metamorphic epoch of food and feedstuff, it is nontoxic to people and animals, it is the natural antiseptic agent of high effect nontoxic.Recently Nisin is also found
Can be used to treat gastric duodenal ulcer, oral ulcer and dermatosiss, have effect to the bovine mastitis of milch cow, with medicine
Value, development prospect is wide.
In the prior art, commonly use fermented feed carries out together different bacterium using lactic acid bacteria, sporeformer bar or yeast etc.
Kind mixed fermentation, this technology is intended to make various bacterium synergism, plays effect of various microorganisms, but actually by
It is different in different bacterium optimum fermentation condition, therefore be difficult to coordinate during practical operation, usually attend to one thing and lose sight of another, and damage final fermentation
The quality of feedstuff.
The content of the invention
It is an object of the present invention to provide a kind of magnetic field assisted fermentation Feed Production Technology rich in natural antibacterial agent, by using
The process, reduces the cost of feedstuff, improves the safety of feedstuff, also improves the quality of feedstuff.
To reach above-mentioned purpose, the technical solution used in the present invention is：A kind of auxiliary of the magnetic field rich in natural antibacterial agent is sent out
Ferment Feed Production Technology, from can lactic acid producing streptostacin streptococcus acidi lactici as the bacterium that sets out, it is prefabricated by basal medium,
Thalline spreads cultivation, mixed protein feed stuff solid fermentation, obtains fermented feed.
In above-mentioned technical proposal, production craft step is：
1. dregs of rice class culture medium is prefabricated：Rapeseed cake by weight：Cottonseed meal：Bean cake is 0.8~1.5：0.8~1.5：0.8~1.5
Formula is that the dregs of rice class raw material of mixing first mixes with water, and the ratio of dregs of rice class raw material and water is 1:1~1:3 are mixed, then are made with this
On the basis of amount addition sucrose 10%~20%, KH2PO4 2%~5%, under conditions of 115 DEG C, sterilize 20 minutes, Ran Houjin
Row is crushed, and after above-mentioned dregs of rice class material mixing 40 mesh were crushed.
2. basal medium is prefabricated：By weight formula be 10 parts~12 parts of sucrose, 10 parts~12 parts of peptone, yeast
10 parts~14 parts of cream, KH2PO410 parts~15 parts, 2 parts -4 parts of NaCl, MgSO40.2 part~0.5 part, regulation pH value to 6.2~
6.8, at a temperature of 115 DEG C, sterilize 20 minutes；
(3) yeast culture：The streptococcus acidi lactici of 50 ㎎~80 ㎎ is connect with inoculating loop in 50ml~75ml basal mediums,
With the speed shaking flask of 200r/min~300r/min, cultivate 16 hours~20 hours under conditions of 30 DEG C~35 DEG C, by 5%
(volume/weight) is inoculated in dregs of rice class culture medium, and static gas wave refrigerator 48 hours~52 hours, obtain under conditions of 30 DEG C~35 DEG C
To the yeast culture dregs of rice；
(4) batch mixing：The yeast culture dregs of rice containing streptococcus acidi lactici after culture are mixed simultaneously with rapeseed cake, cottonseed meal and bean cake
Close, by weight, 10 parts~15 parts of the yeast culture dregs of rice, 25 parts~40 parts of rapeseed cake, cottonseed meal 25-40 parts, bean cake 25-50 parts must mix
Solid fermentation raw materials are closed, by solid fermentation raw materials：Water is 1：0.3~0.5 proportioning adds water, obtains solid-state fermentation culture medium；
40 mesh were crushed after above-mentioned dregs of rice class material mixing.
(5) magnetic field auxiliary solid fermentation：The raw material of above-mentioned mixing is placed in the field region of alternation, and magnetic induction 0.3T~
1.5T, frequency 50Hz~1000Hz is fermented 2 days~3 days under conditions of 30 DEG C~35 DEG C of temperature, and period stirs one every 12h
It is secondary；
(6) finished product：After the completion of fermentation, take 1g fermentation dregs of rice thing feedstuffs and be added to 9mL, in 0.02mol/L hydrochloric acid weak solutions, or
Fermented product dregs of rice feedstuff is suitably diluted with 0.02mol/L dilute hydrochloric acid (is diluted to newborn chain of its antibacterial circle diameter in 5~100IU/mL
Between the antibacterial circle diameter of bacterium peptide titer), after boiling water bath is heated 5 minutes, with 10 points of the centrifugation of rotating speed 5000g/min
Clock, takes W of supernatant agar diffusion method and determines its biological value, unit：IU/g fermentation dregs of rice things, potency now is 1600-
During 2000IU/g, as finished product.
In above-mentioned technical proposal, the dregs of rice class raw material formula by weight of mixing is 30 parts~40 parts of rapeseed cake, 30 parts of cottonseed meal
~40 parts, 40 parts~60 parts of bean cake, the wherein protein content of rapeseed cake be 32%~36%, carbohydrate content be 50%~
58%, fat content is 3%~5%；The protein content of cottonseed meal be 34%~40%, carbohydrate content be 50%~
60%, fat content is 3%~6%；The protein content of bean cake is 36%~48%, and carbohydrate content is 40%-
45%, fat content is 3%~10%.
Because above-mentioned technical proposal is used, the present invention has compared with prior art following advantages：
1. traditional antibiotic addition feedstuff is different from the present invention, using the streptococcus acidi lactici solid fermentation for producing Nisin
Mixing rapeseed cake, the feedstuff of bean cake and cottonseed meal, with natural antibacterial and technology can improve the palatability and efficiency of feed utilization of adjuvant,
Adding by a certain percentage to mixed feed can make feedstuff not use antibiotic, acidulant etc., reduce the cost of feedstuff, improve
The safety of feedstuff.
2. feed fermentation is carried out only with streptococcus acidi lactici in the present invention, improve the quality of product；
3. magnetic field has cell biocompatibility in the present invention, it can make cellular morphology, DNA, RNA, protein synthesis, across
Film transhipment, enzymatic activity and biological heredity etc. produce significant changes.The magnetic field of alternation is by the transition metal in albumen and enzyme
The function influence enzymatic activity of ion, and then affect the metabolism that enzyme and cell are participated in react.Magnetic field turns to biomembranous ion
The impact of capacity power can cause the change of some physiology and biochemical process, so as to affect the correlated process of bioelectric.It is extremely low
Frequently the target spot that the low-intensity magnetic field of (ELF) acts on cell is cell membrane first.Research discovery, reaction of the living organism to the action of a magnetic field
Middle cell membrane plays Main Function.Magnetic field can also change the CHARGE DISTRIBUTION on film surface, adjust and receive ligand binding, activation signal conduction
System, affects the electrical characteristics of ion channel on cell membrane, ultimately results in the change of cell activities, and then regulates and controls sending out for thalline
Ferment process, ensure that fermented quality, improve feed quality.
Specific embodiment
With reference to embodiment, the invention will be further described：
Embodiment one：A kind of magnetic field assisted fermentation Feed Production Technology rich in natural antibacterial agent, the mixing dregs of rice class of employing
Raw material formula by weight is 30 parts of rapeseed cake, 30 parts of cottonseed meal, 40 parts of bean cake.
Dregs of rice class culture medium is prefabricated：By weight formula is the dregs of rice class raw material and water of mixing first by 1:1 is mixed, then with
This adds sucrose 12%, KH as datum quantity2PO43%th, in 115 DEG C, sterilize 20min；Crushed after above-mentioned dregs of rice class material mixing
40 mesh.
Basal medium is prefabricated：By weight formula be 12 parts of sucrose, 10 parts of peptone, 13 parts of yeast extract, KH2PO410
Part, NaCl3 parts, MgSO40.4 part, pH 6.5 is adjusted, in 115 DEG C, sterilize 20min；
Yeast culture：The streptococcus acidi lactici of 60mg is connect with inoculating loop in 60mL basal mediums, 250r/min shaking flasks, 33
DEG C culture 18h, being inoculated in above-mentioned dregs of rice class culture medium by 5% (volume/weight), 33 DEG C of static gas wave refrigerator 52h；
Batch mixing：Dregs of rice class culture medium containing streptococcus acidi lactici after culture is mixed simultaneously with rapeseed cake, cottonseed meal and bean cake,
By weight, 12 parts of the yeast culture dregs of rice, 30 parts of rapeseed cake, 30 parts of cottonseed meal, 28 parts of bean cake；Crushed after above-mentioned dregs of rice class material mixing
40 mesh.
Magnetic field aids in solid fermentation：The raw material of above-mentioned mixing is placed in the field region of alternation, magnetic induction 1.3T, frequency
400Hz, in temperature, 35 DEG C ferment 3 days, and period is stirred once every 12h；
Finished product：After the completion of fermentation, take 1g fermentation dregs of rice thing feedstuffs and be added to 9mL, in 0.02mol/L hydrochloric acid weak solutions, or use
Fermented product dregs of rice feedstuff is suitably diluted and (is diluted to its antibacterial circle diameter 5IU/mL~100IU/mL's by 0.02mol/L dilute hydrochloric acid
Between the antibacterial circle diameter of nisin titer), after boiling water bath heating 5min, 5000g/min centrifugation 10min take supernatant
Agar diffusion method determines its biological value, unit：IU/g ferments dregs of rice thing, when potency now is 1600IU/g, as finished product.
Embodiment 2
The mixing dregs of rice class raw material formula by weight for adopting is for 10 parts of rapeseed cake, 11 parts of cottonseed meal, 12 parts of bean cake.
Dregs of rice class culture medium is prefabricated：By weight formula is the dregs of rice class raw material and water of mixing first by 1:2 are mixed, then with
This adds sucrose 16%, KH as datum quantity2PO45%th, in 115 DEG C, sterilize 20min；Crushed after above-mentioned dregs of rice class material mixing
40 mesh.
Basal medium is prefabricated：By weight formula be 12 parts of sucrose, 11 parts of peptone, 13 parts of yeast extract, KH2PO412
Part, NaCl3 parts, MgSO40.2 part, pH6.3 is adjusted, in 115 DEG C, sterilize 20min；
Yeast culture：The streptococcus acidi lactici of 74mg is connect with inoculating loop in 70mL basal mediums, 230r/min shaking flasks, 35
DEG C culture 20h, being inoculated in dregs of rice class culture medium by 5% (volume/weight), 32 DEG C of static gas wave refrigerator 46h；
Batch mixing：Dregs of rice class culture medium containing streptococcus acidi lactici after culture is mixed simultaneously with rapeseed cake, cottonseed meal and bean cake,
By weight, 15 parts of the yeast culture dregs of rice, 20 parts of rapeseed cake, 20 parts of cottonseed meal, 45 parts of bean cake；
Magnetic field aids in solid fermentation：The raw material of above-mentioned mixing is placed in the field region of alternation, magnetic induction 0.8T, frequency
100Hz, ferments 2 days in 32 DEG C, and period is stirred once every 12h；40 mesh were crushed after above-mentioned dregs of rice class material mixing.
Finished product：After the completion of fermentation, take 1g fermentation dregs of rice thing feedstuffs and be added to 9mL, in 0.02mol/L hydrochloric acid weak solutions, or use
Fermented product dregs of rice feedstuff is suitably diluted and (is diluted to newborn chain bacterium of its antibacterial circle diameter in 5~100IU/mL by 0.02mol/L dilute hydrochloric acid
Between the antibacterial circle diameter of peptide titer), after boiling water bath heating 5min, 5000g/min centrifugation 10min take W of supernatant agar expansion
Arching pushing determines its biological value, unit：IU/g ferments dregs of rice thing, when potency now is 1800IU/g, as finished product.
In the present invention, streptococcus acidi lactici is that a kind of saccharide that can decompose produces lactic acid, the probiotic bacteria of Nisin, facultative to detest
Oxide growth, has certain toleration to the sour environment in stomach.In animal body by biological antagonist, reduce pH value, prevent and
Suppress the intrusion and field planting of pathogenic bacterium, the harmful substances such as nitrous ammonia, ammonia, indole and scatol are reduced, so as to maintain intestinal just
Normal colony balance.At present, using the feed product of the streptococcus acidi lactici solid fermentation mixing rapeseed cake, bean cake and cottonseed meal for producing Nisin
There is no.The secondary material obtained after grain class or other agricultural product squeezing processing can have sour-sweet fragrance Jing after streptococcus acidi lactici fermentation
Abnormal smells from the patient, pH value declines, and can be effectively improved the palatability of these adjuvants, promotes growth of animals or poultry, puts on weight, improves food conversion ratio
And production performance.Meanwhile, streptococcus acidi lactici is to setting up and maintains the normal microecological balance of intestinal to play great role, can carry
High feed digestion utilization rate.Streptococcus acidi lactici nonspecific immunity stimulation can improve the immunocompetence of poultry.Sent out using solid-state
Ingredient of the dregs of rice class feedstuff after ferment as pigletss diet, it is also possible to reduce stress when pigletss are weaned, can strengthen
The resistances against diseases of body, reduce Incidence of Diarrhea, improve the daily gain of piglet.Antibiotic, acidulant simultaneously can be reduced to add
Dosage, reduces feed cost.This technology is utilized produces Nisin streptococcus acidi lactici solid fermentation rapeseed cake, bean cake and cottonseed meal mixed fodder,
Apply the alternating magnetic field of helmholtz coil production in sweat, obtain a kind of fermented feed with natural antibacterial and produce
Product, effectively reduce application of the antibiotic in feedstuff, while improve the economic worth of all kinds of dregs of rice class feedstuffs.
Meanwhile, compared with multiple bacteria compound fermentation feedstuff, fermentation condition is more easy to control, and nisin (Nisin) is more
Be conducive to making tunning be rich in antibiotic substance.
Claims (3)
1. a kind of magnetic field assisted fermentation Feed Production Technology rich in natural antibacterial agent, it is characterised in that：From energy lactic acid producing chain
Used as the bacterium that sets out, prefabricated by basal medium, thalline spreads cultivation the streptococcus acidi lactici of coccus element, and mixed protein feed stuff solid-state is sent out
Ferment, obtains fermented feed.
2. the magnetic field assisted fermentation Feed Production Technology rich in natural antibacterial agent according to claim 1, it is characterised in that：
Production craft step is：
1. dregs of rice class culture medium is prefabricated：Rapeseed cake by weight：Cottonseed meal：Bean cake is 0.8~1.5：0.8~1.5：0.8~1.5 formula
Dregs of rice class raw material for mixing first mixes with water, and the ratio of dregs of rice class raw material and water is 1:1~1:3 are mixed, then in this, as base
Quasi- amount addition sucrose 10%~20%, KH2PO4 2%~5%, under conditions of 115 DEG C, sterilize 20 minutes, then carry out powder
It is broken, crushed 40 mesh after above-mentioned dregs of rice class material mixing.
2. basal medium is prefabricated：By weight formula be 10 parts~12 parts of sucrose, 10 parts~12 parts of peptone, yeast extract 10
Part~14 parts, KH2PO410 parts~15 parts, 2 parts -4 parts of NaCl, MgSO40.2 part~0.5 part, pH value is adjusted to 6.2~6.8,
At a temperature of 115 DEG C, sterilize 20 minutes；
(3) yeast culture：The streptococcus acidi lactici of 50 ㎎~80 ㎎ is connect with inoculating loop in 50ml~75ml basal mediums, with
The speed shaking flask of 200r/min~300r/min, cultivates 16 hours~20 hours, by 5% (body under conditions of 30 DEG C~35 DEG C
Product/weight) be inoculated in dregs of rice class culture medium, static gas wave refrigerator 48 hours~52 hours, obtain under conditions of 30 DEG C~35 DEG C
The yeast culture dregs of rice；
(4) batch mixing：The yeast culture dregs of rice containing streptococcus acidi lactici after culture are mixed simultaneously with rapeseed cake, cottonseed meal and bean cake,
By weight, 10 parts~15 parts of the yeast culture dregs of rice, 25 parts~40 parts of rapeseed cake, cottonseed meal 25-40 parts, bean cake 25-50 parts must mix
Solid fermentation raw materials, by solid fermentation raw materials：Water is 1：0.3~0.5 proportioning adds water, obtains solid-state fermentation culture medium；On
State and crushed 40 mesh after dregs of rice class material mixing.
(5) magnetic field auxiliary solid fermentation：The raw material of above-mentioned mixing is placed in the field region of alternation, and magnetic induction 0.3T~
1.5T, frequency 50Hz~1000Hz is fermented 2 days~3 days under conditions of 30 DEG C~35 DEG C of temperature, and period stirs one every 12h
It is secondary；
(6) finished product：After the completion of fermentation, take 1g fermentation dregs of rice thing feedstuffs and be added to 9mL, in 0.02mol/L hydrochloric acid weak solutions, or use
Fermented product dregs of rice feedstuff is suitably diluted and (is diluted to newborn chain bacterium of its antibacterial circle diameter in 5~100IU/mL by 0.02mol/L dilute hydrochloric acid
Between the antibacterial circle diameter of peptide titer), after boiling water bath is heated 5 minutes, with the centrifugation 10 minutes of rotating speed 5000g/min,
Take W of supernatant agar diffusion method and determine its biological value, unit：IU/g fermentation dregs of rice things, potency now is 1600-2000IU/g
When, as finished product.
3. the magnetic field assisted fermentation Feed Production Technology rich in natural antibacterial agent according to claim 2, it is characterised in that：
The dregs of rice class raw material formula by weight of mixing be 30 parts~40 parts of rapeseed cake, 30 parts~40 parts of cottonseed meal, 40 parts~60 parts of bean cake, its
The protein content of middle rapeseed cake is 32%~36%, and carbohydrate content is 50%~58%, and fat content is 3%~5%；
The protein content of cottonseed meal is 34%~40%, and carbohydrate content is 50%~60%, and fat content is 3%~6%；Bean
The protein content of the dregs of rice is 36%~48%, and carbohydrate content is 40%-45%, and fat content is 3%~10%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108740296A (en) * | 2018-06-23 | 2018-11-06 | 山东鸿毅食品有限公司 | A kind of feed and preparation method thereof for boiler duck feeding |
| CN109486724A (en) * | 2018-12-26 | 2019-03-19 | 江苏牧之歌生态农业科技有限公司 | A kind of synchronization preparation process and its feed applications mixing probiotics leaven |
| CN111109565A (en) * | 2019-10-25 | 2020-05-08 | 新疆红旗坡农业发展集团有限公司 | Strong-fragrance probiotic low-sugar apple jam and preparation method thereof |
| CN113575684A (en) * | 2021-07-30 | 2021-11-02 | 玉林师范学院 | Preparation method of wheat seedling yoghourt |
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| CN104372061A (en) * | 2014-10-21 | 2015-02-25 | 浙江省林业科学研究院 | Preparation method of zinc-enriched liquid fermentation product of phellinus linteus |
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| CN104543371A (en) * | 2015-01-17 | 2015-04-29 | 东北农业大学 | Method for enzymolysis of soybean meal by employing gas-liquid-solid magnetically stabilized fluidized bed |
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| US20020176904A1 (en) * | 1996-07-31 | 2002-11-28 | Julien William E. | Antiprotozal composition |
| CN103642709A (en) * | 2013-08-28 | 2014-03-19 | 浙江工业大学 | Strain of Streptococcus lactis and applications in preparation of feed additives through microbial fermentation |
| CN104372060A (en) * | 2014-08-11 | 2015-02-25 | 江苏大学 | Method for preparation of oligopeptides by solid state fermentation cake |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108740296A (en) * | 2018-06-23 | 2018-11-06 | 山东鸿毅食品有限公司 | A kind of feed and preparation method thereof for boiler duck feeding |
| CN109486724A (en) * | 2018-12-26 | 2019-03-19 | 江苏牧之歌生态农业科技有限公司 | A kind of synchronization preparation process and its feed applications mixing probiotics leaven |
| CN111109565A (en) * | 2019-10-25 | 2020-05-08 | 新疆红旗坡农业发展集团有限公司 | Strong-fragrance probiotic low-sugar apple jam and preparation method thereof |
| CN113575684A (en) * | 2021-07-30 | 2021-11-02 | 玉林师范学院 | Preparation method of wheat seedling yoghourt |
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