CN104824426A - Preparation method of sheep feed additive fermented by probiotic - Google Patents
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Abstract
The invention discloses a preparation method of a sheep feed additive fermented by probiotic. The preparation method comprises the following steps: (1) taking corn bract, locust leaf, elm leaf, mulberry leaf, and alfalfa as the raw materials, washing, drying in the shade, and chopping; (2) evenly mixing the raw materials together, adding water, and grinding to prepare slurry; (3) adding bacillus subtilis and aspergillus oryzae into the slurry, stirring, sealing, and fermenting to obtain fermentation broth; (4) inoculating a lactobacillus acidophilus nutritional solution to the fermentation broth to obtain a primary fermentation broth; (5) adding sodium chloride into the primary fermentation broth, fully stirring, carrying out fermentation at a normal temperature to obtain a secondary fermentation broth, filtering, recycling the filtrate, and saving the filtrate at a temperature of 2 to 10 DEG C. The sheep feed additive can improve the palatability of feeds, strengthen the permanent planting ability of probiotics in intestines, promote the digestion and absorption, enhance the immunity and performance on resisting diseases, and reduce the morbidity; moreover, the raw material resources are stable, and the prices are cheap.
Description
Technical field
The invention belongs to field of feed additive technology, be specifically related to a kind of preparation method of sheep feed additive of probiotics fermention.
Background technology
Mutton is one of main meat of eating of China people, and mutton is more delicate than the meat of pork, and all fewer than the fat of pork and beef, cholesterol level.Relative pork, mutton protein content is more, and fat content is less.Cobastab
1, B
2, B
6and iron, zinc, selenium content rather abundant.In addition, mutton fine and tender taste, easily digests and assimilates, and eats mutton more and contributes to improving body immunity.Mutton heat is higher than beef, always by as one of autumn and winter resisting cold and the important foodstuffs of nourishing.Therefore, the development of sheep husbandry goes from strength to strength, along with the development of sheep husbandry, various sheep feed addictive constantly floods the market, quite a few employing antibiotic of existing sheep feed additive and trace element are formed, although the feed addictive of this type can prevent the generation of disease after a procedure effectively, improve breeding performonce fo animals, improve sheep raising benefit, but the Long-Time Service of antibiotics additive easily causes many pathogens to produce drug resistance, livestock and poultry alimentary tract normal flora is lacked of proper care, immunologic function of livestock and birds is declined, serious medicament residue is produced in sheep body, grievous injury sheep and the mankind's is healthy.Along with antibiotics additive uses the problems brought to be seen in report, market starts occur comparatively safe Chinese herbal feed additive, some Chinese herbal medicine price costly, aquaculture cost is caused to rise, in addition, containing residues of pesticides and indefinite toxic and side effect in some Chinese herbal medicines, cause the reduction of quality of mutton, larger threat is formed to food security simultaneously.
Probio is the active microorganism that a class is useful to host, is to be colonizated in human body intestinal canal, reproductive system, can produce definite health efficacy thus improve host's microecological balance, plays the active beneficial microorganism general name of beneficial effect.Bacterium useful in human body, animal body or fungi mainly contain: clostridium butyricum, lactic acid bacteria, Bifidobacterium, lactobacillus acidophilus, actinomyces, saccharomycete etc.The composite reactive probio of the most powerful product of the function studied in the world at present mainly above each quasi-microorganism composition, it is widely used in bioengineering, industrial or agricultural, food security and life and health field.Therefore a kind of feed addictive of probiotics fermention is studied to replace existing antibiotic and pure Chinese medicine composition additive, very necessary to livestock and poultry cultivation.
Summary of the invention
For problems of the prior art, the object of the invention is the preparation method at the sheep feed additive providing a kind of probiotics fermention.
The present invention is realized by the following technical programs:
The preparation method of the sheep feed additive of described a kind of probiotics fermention, is characterized in that this preparation method is made up of following steps:
1) after getting fresh bracteal leaf of corn, Folium sophorae, leaf of elm tree, mulberry leaves, Alfalfa as raw material, clean, drying in the shade, chopping, for subsequent use;
2) each raw material of step 1) process of learning from else's experience respectively mixes, and obtain compound, add water in compound defibrination, obtains slurries;
3) in step 2) add bacillus subtilis and aspergillus oryzae respectively in the slurries of gained, stir, sealing, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtain fermenation raw liquid;
4) inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 4-6g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, stops fermenting to obtain one time fermentation liquid;
5) in step 4) gained one time fermentation liquid, add 1-4%(w/v) sodium chloride, after fully stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, filtered by secondary fermentation liquid, filtrate is preserved under being recovered in the temperature conditions of 2-10 DEG C.
The preparation method of the sheep feed additive of described a kind of probiotics fermention, is characterized in that in step 1), each raw material is chopped into the segment of 0.5-1cm length.
The preparation method of the sheep feed additive of described a kind of probiotics fermention, is characterized in that step 2) in compound each raw material form by following parts by weight: bracteal leaf of corn 20-40 part, Folium sophorae 30-50 part, leaf of elm tree 10-30 part, mulberry leaves 5-20 part, Alfalfa 20-40 part.
The preparation method of the sheep feed additive of described a kind of probiotics fermention, is characterized in that in compound, each raw material forms by following parts by weight: bracteal leaf of corn 30-40 part, Folium sophorae 30-40 part, leaf of elm tree 20-30 part, mulberry leaves 5-15 part, Alfalfa 20-30 part.
The preparation method of the sheep feed additive of described a kind of probiotics fermention, is characterized in that in compound, each raw material forms by following parts by weight: bracteal leaf of corn 35 parts, Folium sophorae 35 parts, leaf of elm tree 25 parts, mulberry leaves 10 parts, Alfalfa 25 parts.
The preparation method of the sheep feed additive of described a kind of probiotics fermention, is characterized in that step 2) in the addition of water identical with the weight of compound.
The preparation method of the sheep feed additive of described a kind of probiotics fermention, is characterized in that total interpolation weight of bacillus subtilis and aspergillus oryzae accounts for the 2-4% of slurries gross weight, and the interpolation of bacillus subtilis and aspergillus oryzae is than being 1:1.
The preparation method of the sheep feed additive of described a kind of probiotics fermention, is characterized in that the inoculum concentration of lactobacillus acidophilus nutrient solution in step 4) is inoculate 5g in every 100g fermenation raw liquid.
The preparation method of the sheep feed additive of described a kind of probiotics fermention, is characterized in that in step 5), the addition of sodium chloride in zymotic fluid is 3%(w/v).
The preparation method of the sheep feed additive of described a kind of probiotics fermention, is characterized in that: when feeding, and the amount adding this feed addictive is 1.5% of sheep feed total amount.
Bracteal leaf of corn, also corn leafage is claimed, that the bag of corn ear outside is by part, source is wide, cheap, it contains a large amount of celluloses, a certain amount of general flavone, starch and other composition such as polysaccharide, monose, and bracteal leaf of corn, after fermentation process fermentation of the present invention, can increase substantially protein content in tunning; Folium sophorae is containing crude protein, crude fat, crude fibre, calcium, phosphorus etc., and containing abundant chlorophyll, vitamin C, the fresh leaf of Folium sophorae is after fermentation process process of the present invention, saccharified pig feed is made after coordinating with leaf of elm tree, mulberry leaves, effectively can improve the output of milk of ewe and improve the output of mutton and wool, shortening feed period; Leaf of elm tree contains higher crude protein, and NFE and considerable mineral matter and carrotene, after fermentation process of the present invention, be used from other components matching one and promote growing of sheep, improves the resistance of sheep body; Mulberry leaves has the effect of resisting pathogenic microbes, and mulberry leaves active component all has certain inhibitory action to staphylococcus aureus, beta hemolytic streptococcus, corynebacterium diphtheriae and Escherichia coli, has antiinflammatory action, diuresis; Being of high nutritive value of Alfalfa, containing rich in protein, crude fibre, vitamin and trace element.In addition, the various pigments contained in Alfalfa, have improvement result to the quality of animal product.The active material contained in clover, improves immunity of organisms as clover flavones, alfalfa saponin and Alfalfa Polysaccharide etc. have, and improves the effect of the anti-oxidant grade of body, all has facilitation to growing of animal.
Compared with existing feed addictive, the present invention has the following advantages:
(1) in sheep feed, obviously can reduce bitter taste with the use of, Folium sophorae as additive after fermentation, improve the palatability of feed together with bracteal leaf of corn;
(2) each raw material is after fermentation process, is convenient to the performance of active component pharmacological action in each raw material, and then strengthens the colonization ability of beneficial bacterium in enteron aisle, and promotion is digested and assimilated, the resistance against diseases of develop immunitypty, raising body, the reduction incidence of disease;
(3) between each material effective component, compatibility is reasonable, and effect is complementary, can produce good synergistic function;
(4) raw materials enjoy stable sources, cheap, production technology is simple, has no side effect and bad reaction.
Detailed description of the invention
By following specific embodiment, the invention will be further described.
Embodiment 1
A preparation method for the sheep feed additive of probiotics fermention, after getting fresh bracteal leaf of corn, Folium sophorae, leaf of elm tree, mulberry leaves, Alfalfa as raw material, clean, drying in the shade, is chopped into the segment of 0.5-1cm length, for subsequent use, get the bracteal leaf of corn after chopping 35 parts, Folium sophorae 35 parts, leaf of elm tree 25 parts, mulberry leaves 10 parts, Alfalfa 25 parts, compound is obtained after mixing, the water mill slurry of same weight is added in compound, obtain slurries, bacillus subtilis and the aspergillus oryzae of same weight is added respectively in slurries, both account for 3% of slurries gross weight by addition, stir after having added, sealing, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtain fermenation raw liquid, inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 5g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, termination is fermented to obtain one time fermentation liquid, with regard to 3%(w/v in one time fermentation liquid) sodium chloride, after abundant stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, secondary fermentation liquid is filtered, filtrate is preserved under reclaiming the temperature conditions with 2-10 DEG C.When feeding, in 100 parts of conventional feeds, add this feed addictive 1.5 parts, can feed after stirring.
Embodiment 2
A preparation method for the sheep feed additive of probiotics fermention, after getting fresh bracteal leaf of corn, Folium sophorae, leaf of elm tree, mulberry leaves, Alfalfa as raw material, clean, drying in the shade, is chopped into the segment of 0.5-1cm length, for subsequent use, get the bracteal leaf of corn after chopping 20 parts, Folium sophorae 50 parts, leaf of elm tree 10 parts, mulberry leaves 20 parts, Alfalfa 40 parts, compound is obtained after mixing, the water mill slurry of same weight is added in compound, obtain slurries, bacillus subtilis and the aspergillus oryzae of same weight is added respectively in slurries, both account for 2% of slurries gross weight by addition, stir after having added, sealing, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtain fermenation raw liquid, inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 6g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, termination is fermented to obtain one time fermentation liquid, with regard to 1%(w/v in one time fermentation liquid) sodium chloride, after abundant stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, secondary fermentation liquid is filtered, filtrate is preserved under reclaiming the temperature conditions with 2-10 DEG C.When feeding, in 100 parts of conventional feeds, add this feed addictive 1.5 parts, can feed after stirring.
Embodiment 3
A preparation method for the sheep feed additive of probiotics fermention, after getting fresh bracteal leaf of corn, Folium sophorae, leaf of elm tree, mulberry leaves, Alfalfa as raw material, clean, drying in the shade, is chopped into the segment of 0.5-1cm length, for subsequent use, get the bracteal leaf of corn after chopping 30 parts, Folium sophorae 40 parts, leaf of elm tree 20 parts, mulberry leaves 15 parts, Alfalfa 20 parts, compound is obtained after mixing, the water mill slurry of same weight is added in compound, obtain slurries, bacillus subtilis and the aspergillus oryzae of same weight is added respectively in slurries, both account for 4% of slurries gross weight by addition, stir after having added, sealing, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtain fermenation raw liquid, inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 4g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, termination is fermented to obtain one time fermentation liquid, with regard to 2%(w/v in one time fermentation liquid) sodium chloride, after abundant stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, secondary fermentation liquid is filtered, filtrate is preserved under reclaiming the temperature conditions with 2-10 DEG C.When feeding, in 100 parts of conventional feeds, add this feed addictive 1.5 parts, can feed after stirring.
Embodiment 4
A preparation method for the sheep feed additive of probiotics fermention, after getting fresh bracteal leaf of corn, Folium sophorae, leaf of elm tree, mulberry leaves, Alfalfa as raw material, clean, drying in the shade, is chopped into the segment of 0.5-1cm length, for subsequent use, get the bracteal leaf of corn after chopping 40 parts, Folium sophorae 30 parts, leaf of elm tree 30 parts, mulberry leaves 5 parts, Alfalfa 30 parts, compound is obtained after mixing, the water mill slurry of same weight is added in compound, obtain slurries, bacillus subtilis and the aspergillus oryzae of same weight is added respectively in slurries, both account for 3.5% of slurries gross weight by addition, stir after having added, sealing, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtain fermenation raw liquid, inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 5.5g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, termination is fermented to obtain one time fermentation liquid, with regard to 4%(w/v in one time fermentation liquid) sodium chloride, after abundant stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, secondary fermentation liquid is filtered, filtrate is preserved under reclaiming the temperature conditions with 2-10 DEG C.When feeding, in 100 parts of conventional feeds, add this feed addictive 1.5 parts, can feed after stirring.
Embodiment 5
A preparation method for the sheep feed additive of probiotics fermention, after getting fresh bracteal leaf of corn, Folium sophorae, leaf of elm tree, mulberry leaves, Alfalfa as raw material, clean, drying in the shade, is chopped into the segment of 0.5-1cm length, for subsequent use, get the bracteal leaf of corn after chopping 25 parts, Folium sophorae 45 parts, leaf of elm tree 15 parts, mulberry leaves 8 parts, Alfalfa 35 parts, compound is obtained after mixing, the water mill slurry of same weight is added in compound, obtain slurries, bacillus subtilis and the aspergillus oryzae of same weight is added respectively in slurries, both account for 2.5% of slurries gross weight by addition, stir after having added, sealing, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtain fermenation raw liquid, inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 5.5g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, termination is fermented to obtain one time fermentation liquid, with regard to 3%(w/v in one time fermentation liquid) sodium chloride, after abundant stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, secondary fermentation liquid is filtered, filtrate is preserved under reclaiming the temperature conditions with 2-10 DEG C.When feeding, in 100 parts of conventional feeds, add this feed addictive 1.5 parts, can feed after stirring.
Test example 1
Select the sheep of body weight difference within 5 jin that 120 healthy, be divided into 6 groups at random, often organize 20, the feed of experimental group 1 feeding embodiment 1, the feed of experimental group 2 feeding embodiment 2, the feed of experimental group 3 feeding embodiment 3, the feed of experimental group 4 feeding embodiment 4, the feed of experimental group 5 feeding embodiment 5, experimental group 6 is control group, the not additivated normal diet of feeding.Feeding effect is in table 1:
Table 1: feeding effect
as can be seen from Table 1, the indices of experimental group 1-5 is obviously better than control group, and the feed intake of sheep adds 20.7%, 19.0%, 15.9%, 19.3%, 17.3% respectively; Feed waste rate reduces 43.3% respectively,
37.5%, 34.4%, 42.4%, 34.7%; Daily gain improves 50%, 28.9%, 34.2%, 26.3%, 44.7% respectively; M & M obviously reduces.
Test example 2
Select healthy anosis, lambing date, parity, ewe 60 that lactation amount is close, be divided into 6 groups at random, often organize 10,1-5 group is fed the feed of embodiment 1-5 respectively, feeds for 6 groups and does not add the conventional feed of this additive, from the 10th day of on-test, start record, record 30 days altogether, every bu morning, afternoon milk for twice, and record the output of milk one by one, the results are shown in Table 2:
Table 2
As can be seen from Table 2, the output of milk of 1-5 group is apparently higher than the output of milk of 6 groups, and total output of milk improves 30.7%, 29.0%, 22.7%, 12.2%, 20.6% respectively.
Claims (10)
1. a preparation method for the sheep feed additive of probiotics fermention, is characterized in that this preparation method is made up of following steps:
1) after getting fresh bracteal leaf of corn, Folium sophorae, leaf of elm tree, mulberry leaves, Alfalfa as raw material, clean, drying in the shade, chopping, for subsequent use;
2) each raw material of step 1) process of learning from else's experience respectively mixes, and obtain compound, add water in compound defibrination, obtains slurries;
3) in step 2) add bacillus subtilis and aspergillus oryzae respectively in the slurries of gained, stir, sealing, in the temperature conditions bottom fermentation 3-10 days of 27-32 DEG C, obtain fermenation raw liquid;
4) inoculating lactobacillus acidophilus's nutrient solution in fermenation raw liquid, inoculum concentration is inoculate 4-6g nutrient solution in every 100g fermenation raw liquid, in the temperature conditions bottom fermentation 1-2 days of 32-36 DEG C, stops fermenting to obtain one time fermentation liquid;
5) in step 4) gained one time fermentation liquid, add 1-4%(w/v) sodium chloride, after fully stirring under normal temperature standing for fermentation 5-10 days, obtain secondary fermentation liquid, filtered by secondary fermentation liquid, filtrate is preserved under being recovered in the temperature conditions of 2-10 DEG C.
2. the preparation method of the sheep feed additive of a kind of probiotics fermention according to right 1, is characterized in that in step 1), each raw material is chopped into the segment of 0.5-1cm length.
3. the preparation method of the sheep feed additive of a kind of probiotics fermention according to right 1, is characterized in that step 2) in compound each raw material form by following parts by weight: bracteal leaf of corn 20-40 part, Folium sophorae 30-50 part, leaf of elm tree 10-30 part, mulberry leaves 5-20 part, Alfalfa 20-40 part.
4. the preparation method of the sheep feed additive of a kind of probiotics fermention according to right 3, is characterized in that in compound, each raw material forms by following parts by weight: bracteal leaf of corn 30-40 part, Folium sophorae 30-40 part, leaf of elm tree 20-30 part, mulberry leaves 5-15 part, Alfalfa 20-30 part.
5. the preparation method of the sheep feed additive of a kind of probiotics fermention according to right 3, is characterized in that in compound, each raw material forms by following parts by weight: bracteal leaf of corn 35 parts, Folium sophorae 35 parts, leaf of elm tree 25 parts, mulberry leaves 10 parts, Alfalfa 25 parts.
6. the preparation method of the sheep feed additive of a kind of probiotics fermention according to claim 1, is characterized in that step 2) in the addition of water identical with the weight of compound.
7. the preparation method of the sheep feed additive of a kind of probiotics fermention according to claim 1, is characterized in that total interpolation weight of bacillus subtilis and aspergillus oryzae accounts for the 2-4% of slurries gross weight, and the interpolation of bacillus subtilis and aspergillus oryzae is than being 1:1.
8. the preparation method of the sheep feed additive of a kind of probiotics fermention according to claim 1, is characterized in that the inoculum concentration of lactobacillus acidophilus nutrient solution in step 4) is inoculate 5g in every 100g fermenation raw liquid.
9. the preparation method of the sheep feed additive of a kind of probiotics fermention according to claim 1, is characterized in that in step 5), the addition of sodium chloride in zymotic fluid is 3%(w/v).
10. the preparation method of the sheep feed additive of a kind of probiotics fermention according to claim 1, is characterized in that: when feeding, and the amount adding this feed addictive is 1.5% of sheep feed total amount.
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CN105341346A (en) * | 2015-12-03 | 2016-02-24 | 合肥五粮泰生物科技股份有限公司 | Preparation method of livestock and poultry woody glycolysis feed |
CN107094990A (en) * | 2017-06-30 | 2017-08-29 | 河南世纪天缘生态科技有限公司 | A kind of wet-milling product method of Woody feed |
CN108740293A (en) * | 2018-05-21 | 2018-11-06 | 何怡江 | The method that microbial fermentation prepares Alfalfa feed |
CN108771006A (en) * | 2018-06-29 | 2018-11-09 | 怀宁县涛涛家庭农场 | A kind of probiotics fermention promotes the feed of domestic animal resistance |
CN108850528A (en) * | 2018-06-29 | 2018-11-23 | 怀宁县涛涛家庭农场 | A kind of pannage and preparation method thereof promoting pig lean meat percentage |
CN108967670A (en) * | 2018-06-12 | 2018-12-11 | 杨杰 | A kind of high-quality green sheep feed and preparation method |
CN110959748A (en) * | 2019-11-14 | 2020-04-07 | 山东鹤来生物科技有限公司 | Liquid fermented feed prepared from waste vegetables and preparation method thereof |
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Cited By (7)
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CN105341346A (en) * | 2015-12-03 | 2016-02-24 | 合肥五粮泰生物科技股份有限公司 | Preparation method of livestock and poultry woody glycolysis feed |
CN107094990A (en) * | 2017-06-30 | 2017-08-29 | 河南世纪天缘生态科技有限公司 | A kind of wet-milling product method of Woody feed |
CN108740293A (en) * | 2018-05-21 | 2018-11-06 | 何怡江 | The method that microbial fermentation prepares Alfalfa feed |
CN108967670A (en) * | 2018-06-12 | 2018-12-11 | 杨杰 | A kind of high-quality green sheep feed and preparation method |
CN108771006A (en) * | 2018-06-29 | 2018-11-09 | 怀宁县涛涛家庭农场 | A kind of probiotics fermention promotes the feed of domestic animal resistance |
CN108850528A (en) * | 2018-06-29 | 2018-11-23 | 怀宁县涛涛家庭农场 | A kind of pannage and preparation method thereof promoting pig lean meat percentage |
CN110959748A (en) * | 2019-11-14 | 2020-04-07 | 山东鹤来生物科技有限公司 | Liquid fermented feed prepared from waste vegetables and preparation method thereof |
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