CN105594993A - Antibacterial active peptide feed additive for pigs and preparing method thereof - Google Patents
Antibacterial active peptide feed additive for pigs and preparing method thereof Download PDFInfo
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Abstract
The invention relates to an antibacterial active peptide feed additive for pigs and a preparing method thereof. The additive is prepared from a component A and a component B; the component A is prepared by fermenting 300-500 parts of corn powder, 20-30 parts of apple slag, 10-15 parts of DDGS, 20-30 parts of corn protein powder and 100-200 parts of bean pulp according to parts by weight through composite fermenting zymophyte; the component B is prepared from 8-10 parts of smoked plum, 12-18 parts of common andrographis herb, 3-5 parts of rhizoma atractylodis macrocephalae, 5-7 parts of moutan bark, 1 part of pepper and 50-80 parts of rice bran according to parts by weight. According to the feed additive, natural raw materials are finely selected, and the natural, green and safe feed additive is prepared by fermenting or extracting, and compared with the traditional antibiotic medicine, the feed additive more accords with the development requirements of modern livestock husbandry and has great production value.
Description
Technical field
The invention belongs to field of fodder, be specifically related to the preparation method of the active pig's feed additive of a kind of antibacterial peptide.
Background technology
In July, 2005, the ground outburst Streptococcus suis epidemic situation causing deaths such as Ziyang, Sichuan Province, inland river, this forces people to chase after againAsk: how long the threat chain of animal abuse of antibiotics has on earth? because antibiotic feed additive can improve animal production efficiency,In raising, edible animal is widely used. 1996, the consumption of whole world antibiotic feed additive has accounted for whole feeds to be addedAdd 45.8% of agent consumption, 50% left and right of antibiotics production total output is for animal husbandry. But, due to lasting low-level feedingAntibiotic, bacterial drug resistance that antibiotic feed additive causes, livestock products medicament residue, irritated toxic reaction and " three cause "Effect waits harm day by day serious, is more and more subject to people's concern and the restriction of many countries. From present situation and development trend,In edible animal, forbidding, limit the use of antibiotic feed additive has become world's common recognition, is trend of the times.
At present, the alternative of fodder antibiotics mainly contains two kinds, one for the microbe additive of feeding be that probiotic (bacterium) changesThe ratio of kind enteric microorganism, suppresses harmful microorganism, promotes growth of animal; Its two for use feeder acidulant, improve enteron aisleThe acid-base value of content, suppresses harmful bacteria, promotes beneficial bacterium growth. But in general, above two kinds of measures are with respect to antibioticEffect still have a certain distance. Various animals have been set up in vivo the microorganism of balance in long-term natural evolution processThe flora symbiosis ecosystem, and set up a set of mechanism that maintains flora stable state in its body, antibacterial peptide is main in this mechanismOne of factor plays very important effect maintaining in normal intestinal flora balance, and may have certain with Intestinal Mucosal ImmunizationRelation. Compared with antibiotic, the bacteriostatic activity of antibacterial peptide is higher, scope of restraining fungi is wider, also has for the production performance of animalSignificantly facilitation; What is more important, antibacterial peptide can not produce any drug resistance; Therefore, develop and develop suitableAntibacterial peptide, by the best substitute that is fodder antibiotics.
Antibacterial peptide refers to be present in organism can resist external microbe infringement, and the small molecule of eliminating vivo mutations cell is manyPeptide, it is the important component part of innate immune system. Antibacterial peptide is made up of 15-45 amino acid residue conventionally, most of bandPositive charge. Multiple biology is as all contained antibacterial peptide in bacterium, insect, plant and vertebrate body. Due to antibiotic resistance andBecoming increasingly conspicuous of residue problem, as the antibiotic substitute of one, antibacterial peptide enjoys because it does not have antibiotic above-mentioned shortcomingPay close attention to. Antibiotic in herding is produced application for a long time, but its long-term use has also caused drug resistance and the serious problems such as residual. CauseThis is necessary to find similar to effects of antibiotics, and there is no the antibiotic substitute of the problems referred to above. Antibacterial peptide is in vivoA kind of antibacterial material extensively existing, is a kind of low-molecular-weight polypeptide, and Gram-positive and negative bacterium are all had to stronger killing and woundingEffect.
Although the useful effect of antibacterial peptide has obtained general confirmation, also obtain accreditation in the industry cycle, in recent years, anti-The application of bacterium peptide is still subject to larger limitation, and particularly, on the one hand, its production technology is immature, and superior strain is difficult to findProduce with industrialization, on the other hand, with high costs, be difficult to promote on a large scale. Based on this, our company through with domestic manyThe long-term joint study of well-known colleges and universities, on the basis of great many of experiments, has obtained a kind of preparation method of antibacterial peptide activated feed, andAnd specifically study the various objects of feeding, for example growth of pig, chicken, duck, ox and aquatic livestock and the custom of ingesting, poorThe further investigation of alienation, has obtained the preparation method of multiple concrete activated peptide forage, for example antibacterial peptide activity involved in the present inventionThe preparation method of pig's feed additive.
Summary of the invention
Be difficult to industrialization and the high defect of production cost in order to make up antibacterial peptide Feed Manufacturing in prior art, the present invention is intended to carryFor the preparation method of the active pig's feed additive of a kind of antibacterial peptide.
For overcoming the above problems, the present invention adopts following technical scheme:
Active pig's feed additive of a kind of antibacterial peptide and preparation method thereof, is made up of component A and B component, wherein component ABy corn flour 300-500 weight portion, pomace 20-30 weight portion, DDGS10-15 weight portion, corn protein powder 20-30Weight portion and dregs of beans 100-200 weight portion are prepared from through the fermentation of composite fermentation microbial inoculum; B component is by dark plum 8-10 weight portion,Herba Andrographitis 12-18 weight portion, bighead atractylodes rhizome 3-5 weight portion, moutan bark 5-7 weight portion, Chinese prickly ash 1 weight portion, rice bran 50-80 weightPart is prepared from; Wherein said composite bacteria agent capable is bacillus licheniformis (Bacilluslichnifarimis), bacillus pumilusAnd candida utili (Candidautilis) composition (Bacillusbrevis).
The preparation method who wherein prepares component A comprises the steps:
Step (1), takes corn flour, pomace, DDGS, corn protein powder and dregs of beans by weight, is mixedAfter pulverized 10-30 mesh sieve, then add the pure water of mixture weight 30%, stir;
Step (2), stirs in the mixture obtaining and adds the composite bacteria agent capable after activation to step (1), continues to stir, andAfter at 27-29 DEG C of fermentation 8-12 hour, in this process in mixture by air, throughput is that 15L air/100kg raisesMaterial mixture/minute; Then adjust the temperature of fermenting mixture, 35-38 DEG C fermentation 8-12 hour, in this process to mixingIn thing by air, throughput be 5L air/100kg forage mixture/minute; Next adjust the temperature of fermentate, at 42-44DEG C standing for fermentation 12-24 hour;
Step (3), the tunning that step (2) is made carries out low temperature drying, then is ground into powder, crosses 30-50 mesh sieve,Obtain component A.
Wherein the preparation method of B component comprises the steps:
Step (one), takes dark plum, Herba Andrographitis, the bighead atractylodes rhizome, moutan bark, Chinese prickly ash and rice bran by weight, and by the tree peony takingSkin and zanthoxylum powder are broken into 10-20 object powder, and the bran powder taking is broken into 50-60 object powder;
Step (two), gets the moutan bark and the Chinese prickly ash that take, the ethanol that is 75% by itself and volumetric concentration by etc. mass mixing, closeClose vessel in heating to 65-72 DEG C, extract 3-5 hour, then filter and obtain alcohol extracting filter residue and alcohol extracting filtrate; By alcohol extracting filter residue andThe dark plum, Herba Andrographitis and the bighead atractylodes rhizome that take mix, and add its mixture quality 1.5-2 water doubly, are heated to 55-60 in closed containerDEG C, extract 8-12 hour, then filter and obtain water extraction filtrate;
Step (three), the bran powder that the alcohol extracting filtrate that step (two) is made and water extraction filtrate and step () make mixes,After stirring, carry out low temperature drying, then be ground into powder, cross 30-50 mesh sieve, obtain B component.
In process of production, the component A making and B component can be mixed to rear packaging sale, can be also independent packagingSell, in the time of the actual use of user, mix.
Preferably, component A is by corn flour 400 weight portions, pomace 25 weight portions, DDGS15 weight portion, corn eggWhite powder 25 weight portions and dregs of beans 150 weight portions are prepared from through the fermentation of composite fermentation microbial inoculum.
Preferably, B component is by dark plum 9 weight portions, Herba Andrographitis 15 weight portions, the bighead atractylodes rhizome 4 weight portions, moutan bark 6 weightPart, Chinese prickly ash 1 weight portion, rice bran 80 weight portions are prepared from
Preferably, as suggestion, but not forcibly, described composite bacteria agent capable is bacillus licheniformis (BacillusLichnifarimis), bacillus pumilus (Bacilluspumilus) and candida utili (Candidautilis) are according to viable bacteriaNumber (CFU) 1:1:1 composition.
Preferably, as suggestion, but not forcibly, described bacillus licheniformis is to protect purchased from Chinese common micro-organisms bacterial classificationHide being numbered CGMCC1.1858 or being numbered the bacillus licheniformis of CGMCC1.7461 of administrative center; Described short and smallBacillus is being numbered CGMCC1.3533 or being numbered purchased from Chinese common micro-organisms culture presevation administrative centerThe bacillus pumilus of CGMCC1.2160, described candida utili is purchased from Chinese common micro-organisms culture presevation managementBeing numbered CGMCC2.1180 or being numbered the candida utili of CGMCC2.1005 of the heart.
The using method of the active pig's feed additive of antibacterial peptide involved in the present invention is: by itself and conventional feedstuff according toAfter the mass ratio of 1:50 mixes, use according to conventional feeding method; Or by feed addictive of the present invention and waterAfter mixing according to the mass ratio of 1:20, the routine that substitutes pig is drunk water.
Based on above summary of the invention, tool of the present invention has the following advantages:
The first, inventor is through repeatedly practising, and draws the best antibacterial peptide fermentation substrate formula of ferment effect and composite fermentation microbial inoculumCombination, research shows, adopts composite fermentation microbial inoculum of the present invention only on fermentation substrate of the present invention, could obtain best sending outFerment effect, antibacterial effect is best, if by itself and conventional feedstuff mixed culture fermentation, the output of antibacterial peptide is extremely low. And,In sweat, also pour into a large amount of painstaking effort of inventor, the nutritional characteristic based on each fermentation stage and growth feature,Draw eventually stepwise fermentation mode involved in the present invention, related generally to the basis of bacterial classification in first stage and the second stage of fermentationThe expansion of metabolism and bacterial classification is numerous, second and three the stage relate generally to the fermentation of the accumulation, particularly phase III of antibacterial peptide, wherein fermentThe fermentation of female bacterium has also produced certain similar fruital taste, also makes final tunning and component A have good local flavor,The cooperation of this fermentation substrate and composite bacteria agent capable and sweat control are observant, are also that applicant pours into a large amount of heartsThe achievement in research of blood does not have in report in the past yet.
The second, the present invention has growth characteristic and the susceptible illness of pig, the mechanism of learning according to Chinese medicine of veterinary medicine, and with reference to traditional Chinese medical science tradition,Selected record medicine to combine, dark plum wherein, Herba Andrographitis, as monarch drug in a prescription, mainly play the effect of sterilization, and the bighead atractylodes rhizome is minister,Moutan bark is assistant, and Chinese prickly ash is for drawing, above medicine uses according to proportioning of the present invention, has reached 1+1 > 2 synergy, it is rightResistance against diseases and immunity in pig have had great lifting, particularly, in the process of feeding, have stopped diarrhea of pigs and dysentery completelyProblem, the speed of growth of pig is more traditional feeding or the fast growth of the pig that normal diet is fed also, and meat is better. DoFor explaining, in the present invention, selected dark plum has desinsection, the convergence function of promoting the production of body fluid, decocting liquid to bacillus anthracis, staphylococcus,Pneumococcus and multiple pathomycete etc. are inhibited; Herba Andrographitis, has function clearing heat and detoxicating, removing pattogenic heat from the blood and toxic material from the body, itsBacillary dysentery is had to significant curative effect, and have no side effect and toxicity, water decoction is to pneumococcus, first streptococcus and catarrh ballBacterium is inhibited, and the illness that multiple virus infections is caused has retarding action; The bighead atractylodes rhizome, has tonifying spleen benefit stomach, eliminating dampness is closedIn effect, its water decoction has the effect that promotes that body weight increases, improves blood middle leukocytes content; Moutan bark, has heat-clearingThe function that cool blood, the row of invigorating blood circulation become silted up, Paeonol wherein has calmness, hypnosis, analgesic effect, and water decoction is to white grapes ballBacterium, hay bacillus, typhoid bacillus etc. have stronger inhibitory action, to shigella dysenteriae, Escherichia coli, Pseudomonas aeruginosa, pneumoniaCoccus, comma bacillus etc. are all inhibited, and virus is also had to certain resistance; Chinese prickly ash, has warming spleen and stomach for dispelling cold, helps digestion and killsThe function of worm. Above medicine is not had an effect simply separately in the present invention, but synergy.
The 3rd, in the present invention, antibacterial peptide feed addictive and Chinese medicine additive are used in combination, also play good cooperative effect,Antibacterial, the anti-infective effect that it reaches is far longer than expection, and it has also played the effect of fabulous promotion pig growth.
In sum, the selected natural material of feed addictive of the present invention, through fermentation or extract made a kind of natural, green,The feed addictive of safety, it compares traditional antibiotic medicine, more meets the demand of Modern Animal Husbandry development, and its productionTechnique is simple, does not need complicated equipment, and production cost is low, but it is effective, and instant effect has great productive value.Through pilot plant test, adopt feed addictive of the present invention only to increase by 2% the cost of feeding, but the direct economic benefit of cultivation canImprove 10-15% left and right, thereby feed addictive of the present invention have great social value and large market prospect.
Detailed description of the invention
Embodiment 1: the active pig's feed additive of a kind of antibacterial peptide, formed by component A and B component, wherein component A isBy corn flour 400 weight portions, pomace 25 weight portions, DDGS15 weight portion, corn protein powder 25 weight portions and dregs of beans 150Weight portion is prepared from through the fermentation of composite fermentation microbial inoculum; B component is by dark plum 9 weight portions, Herba Andrographitis 15 weight portions, the bighead atractylodes rhizome 4Weight portion, moutan bark 6 weight portions, Chinese prickly ash 1 weight portion, rice bran 80 weight portions are prepared from; Wherein said composite bacteria agent capable isBacillus licheniformis (Bacilluslichnifarimis), bacillus pumilus (Bacillusbrevis) and candida utili (CandidaUtilis) composition.
The preparation method who wherein prepares component A comprises the steps:
Step (1), takes corn flour, pomace, DDGS, corn protein powder and dregs of beans by weight, is mixedAfter pulverized 30 mesh sieves, then add the pure water of mixture weight 30%, stir;
Step (2), stirs in the mixture obtaining and adds the composite bacteria agent capable after activation to step (1), continues to stir, andAfter 28 DEG C of fermentations 12 hours, in this process in mixture by air, throughput is that 15L air/100kg feed is mixedCompound/minute; Then adjust the temperature of fermenting mixture, 35 DEG C of fermentations 8 hours, in this process, in mixture, pass through skyGas, throughput be 5L air/100kg forage mixture/minute; Next adjust the temperature of fermentate, 44 DEG C of standing for fermentation12 hours;
Step (3), the tunning that step (2) is made carries out low temperature drying, then is ground into powder, crosses 50 mesh sieves, to obtain final productComponent A.
Wherein the preparation method of B component comprises the steps:
Step (one), takes dark plum, Herba Andrographitis, the bighead atractylodes rhizome, moutan bark, Chinese prickly ash and rice bran by weight, and by the tree peony takingSkin and zanthoxylum powder are broken into 10 object powder, and the bran powder taking is broken into 50 object powder;
Step (two), gets the moutan bark and the Chinese prickly ash that take, the ethanol that is 75% by itself and volumetric concentration by etc. mass mixing, closeClose vessel in heating to 65 DEG C, extract 3 hours, then filter and obtain alcohol extracting filter residue and alcohol extracting filtrate; By alcohol extracting filter residue and takingDark plum, Herba Andrographitis and the bighead atractylodes rhizome mix, add the water of 1.5 times of its mixture qualities, in closed container, be heated to 60 DEG C, carryGet 8 hours, then filter and obtain water extraction filtrate;
Step (three), the bran powder that the alcohol extracting filtrate that step (two) is made and water extraction filtrate and step () make mixes,After stirring, carry out low temperature drying, then be ground into powder, cross 50 mesh sieves, obtain B component.
Component A and B are mixed to the active pig's feed additive of antibacterial peptide described in making.
Embodiment 2: the active pig's feed additive of a kind of antibacterial peptide, it is characterized in that, formed by component A and B component,Wherein component A is by corn flour 500 weight portions, pomace 20 weight portions, DDGS15 weight portion, corn protein powder 20 weightsAmount part and dregs of beans 100 weight portions are prepared from through the fermentation of composite fermentation microbial inoculum; B component is by dark plum 10 weight portions, Herba Andrographitis12 weight portions, the bighead atractylodes rhizome 3 weight portions, moutan bark 7 weight portions, Chinese prickly ash 1 weight portion, rice bran 80 weight portions are prepared from; ItsDescribed in composite bacteria agent capable be bacillus licheniformis (Bacilluslichnifarimis), bacillus pumilus (Bacillusbrevis) andCandida utili (Candidautilis) composition.
The preparation method who wherein prepares component A comprises the steps:
Step (1), takes corn flour, pomace, DDGS, corn protein powder and dregs of beans by weight, is mixedAfter pulverized 20 mesh sieves, then add the pure water of mixture weight 30%, stir;
Step (2), stirs in the mixture obtaining and adds the composite bacteria agent capable after activation to step (1), continues to stir, andAfter 27 DEG C of fermentations 12 hours, in this process in mixture by air, throughput is that 15L air/100kg feed is mixedCompound/minute; Then adjust the temperature of fermenting mixture, 38 DEG C of fermentations 8 hours, in this process, in mixture, pass through skyGas, throughput be 5L air/100kg forage mixture/minute; Next adjust the temperature of fermentate, 42 DEG C of standing for fermentation24 hours;
Step (3), the tunning that step (2) is made carries out low temperature drying, then is ground into powder, crosses 50 object sieves,Obtain component A.
Wherein the preparation method of B component comprises the steps:
Step (one), takes dark plum, Herba Andrographitis, the bighead atractylodes rhizome, moutan bark, Chinese prickly ash and rice bran by weight, and by the tree peony takingSkin and zanthoxylum powder are broken into 20 object powder, and the bran powder taking is broken into 50 object powder;
Step (two), gets the moutan bark and the Chinese prickly ash that take, the ethanol that is 75% by itself and volumetric concentration by etc. mass mixing, closeClose vessel in heating to 72 DEG C, extract 3 hours, then filter and obtain alcohol extracting filter residue and alcohol extracting filtrate; By alcohol extracting filter residue and takingDark plum, Herba Andrographitis and the bighead atractylodes rhizome mix, add the water of 2 times of its mixture qualities, in closed container, be heated to 55 DEG C, extract12 hours, then filter and obtain water extraction filtrate;
Step (three), the bran powder that the alcohol extracting filtrate that step (two) is made and water extraction filtrate and step () make mixes,After stirring, carry out low temperature drying, then be ground into powder, cross 50 object sieves, obtain B component.
Component A and B are mixed to the active pig's feed additive of antibacterial peptide described in making.
Embodiment 3: antibacterial, bacteriostatic experiment
In order to verify the antibacterial activity of product of the present invention, spy arranges following three contrast test groups, wherein:
Check experiment group 1 is by corn flour 400 weight portions, pomace 25 weight portions, DDGS15 weight portion, corn protein powder25 weight portions and dregs of beans 150 weight portions are prepared from through the fermentation of composite fermentation microbial inoculum; Wherein said composite bacteria agent capable is lichens gemmaBacillus (Bacilluslichnifarimis), bacillus pumilus (Bacillusbrevis) and candida utili (Candidautilis)Composition. Its preparation method comprises the steps: step (1), takes by weight corn flour, pomace, DDGS, cornAlbumen powder and dregs of beans, pulverized 30 mesh sieves after being mixed, then add the pure water of mixture weight 30%, stirs allEven; Step (2), stirs in the mixture obtaining and adds the composite bacteria agent capable after activation to step (1), continues to stir, andAfter 32 DEG C of fermentations 48 hours, in this process in mixture by air, throughput is that 15L air/100kg feed is mixedCompound/minute; Step (3), the tunning that step (2) is made carries out low temperature drying, then is ground into powder, crosses 50 mesh sieves,Obtain the product of check experiment group 1.
Check experiment group 2 is by corn flour 400 weight portions, and corn protein powder 25 weight portions and dregs of beans 150 weight portions are through compoundFerment bacteria fermentation is prepared from; Wherein said composite bacteria agent capable is bacillus licheniformis (Bacilluslichnifarimis), short and small gemmaBacillus (Bacillusbrevis) and candida utili (Candidautilis) composition. Its preparation method comprises the steps: stepSuddenly (1), takes corn flour, corn protein powder and dregs of beans by weight, pulverizes 30 mesh sieves after being mixed, and then addsThe pure water that enters mixture weight 30%, stirs; Step (2), stirs in the mixture obtaining and adds work to step (1)Composite bacteria agent capable after change, continues to stir, and then 29 DEG C of fermentations 8 hours, in this process, in mixture, passes through air,Throughput be 15L air/100kg forage mixture/minute; Then adjust the temperature of fermenting mixture, little 38 DEG C of fermentations 8Time, in this process in mixture by air, throughput be 5L air/100kg forage mixture/minute; Next adjustThe temperature of whole fermentate, 44 DEG C of standing for fermentation 12 hours; Step (3), the tunning that step (2) is made carries out lowTemperature is dry, then is ground into powder, crosses 50 mesh sieves, obtains the product of check experiment group 2.
Check experiment group 3 is the A component of the embodiment of the present invention 1.
Two test group are set, are respectively the product of the embodiment of the present invention 1,2, fetch water respectively normal temperature lixiviate embodiment 1,2 withAnd the product of check experiment group 1-3, the ratio of product and water is 1:10, normal temperature extracts 5 hours, and after then leaching liquor being filtered,Filtrate is crossed to the filter of 0.22 micron, obtain aseptic extraction filtrate, get aseptic extraction filtrate respectively to staphylococcus aureus, largeEnterobacteria, salmonella and Shigella carry out inhibition zone test, record the size of inhibition zone, and concrete outcome sees the following form 1:
Table 1 bacteriostatic test result-inhibition zone size (mm)
By above bacteriostatic test can, the product of the embodiment of the present invention 1,2 is for common livestock and poultry, aquatic products susceptible germ-golden yellowLook staphylococcus, Escherichia coli, salmonella and Shigella all have good inhibitory action, and by check experiment group3 more known with check experiment group 1 and 2, in the preparation process of component A of the present invention, fermentation substrate and fermentation parameter forThe output of tunning-antibacterial peptide and antibacterial activity have important impact, are departing under working condition of the present invention, and it is antibacterialSuccessful weakens, and from embodiment 1 and 2 and contrast test group 3 relatively, in product of the present invention, B component andBeing used in conjunction with of component A has significantly the effect of working in coordination with, strengthening for bacteriostatic activity, its bacteriostatic level is significantly better than making separatelyUse A component.
Embodiment 4: detoxification test
100 of pork pigs choosing body weight and be 30-35kg, are divided into 5 groups at random, wherein organize 1 for blank group, adopt ordinary cityThe pork pig feed of selling is fed; Group 2-3 is experimental group, adopts respectively the active pig's feed of antibacterial peptide of embodiment 1,2 to addAgent is fed after mixing according to the mass ratio of 1:50 with common commercially available pork pig feed; Group 4-5 is contrast groups, adopts respectivelyAfter mixing according to the mass ratio of 1:50 with common commercially available pork pig feed by the component A in embodiment 1, B component, raiseHello, to feed and deliver for sale to pork pig, (heating, diarrhoea etc. infect disease summation to statistics infection morbidity number of times, and 1 pig morbidity counts 11 timeInferior), diarrhoea number of times and average body weight gain rate (average weight recruitment/initial average weight * 100%). Concrete outcome sees the following form2：
Table 2 detoxification result of the test
| Infection morbidity number of times | Diarrhoea number of times | Average weight growth rate | |
| Group 1 (blank) | 121 | 78 | 119.2％ |
| Group 2 (embodiment 1) | 5 | 0 | 147.3％ |
| Group 3 (embodiment 2) | 12 | 2 | 135.7％ |
| Group 4 (component A) | 88 | 56 | 121.4％ |
| Group 5 (B component) | 67 | 48 | 119.7％ |
From the result of upper table, compare and do not add additivated situation, add additive of the present invention or embodiment 1 makesComponent A or B component all obtained the effect of improving pig anti-infection ability, and also have some improvement for growing state,But, compare group 4 and group 5, the culture efficiency of group 2-3 obviously will be got well, and its infection frequency and Incidence of Diarrhea are obviously lowIn component A or the B component of feeding separately, and be better than the two and simply superpose, illustrate that component A and B component have played collaborative increasingThe effect of effect.
Embodiment 5: actual feeding
Chosen the 3 family raisers in area, Jiangsu and Zhejiang Provinces, product of the present invention on probation, feeds from 20kg until deliver for sale, anti-through raiserFeedback, product of the present invention can significantly reduce the generation of swine disease, and particularly the generation of infection prevention disease, has reduced antibioticDeng the use of medicine, save medicine cost and the human cost of feeding, meanwhile, the upgrowth situation of pig will be got well than before, andThe average weight of delivering for sale has had certain lifting, does not use or use other additives, the economic benefit of raising before comparingImproved about 15%-22%, and through feedback, the more traditional raising of meat after butchering has had certain improvement, that butchers goes out meatRate has not improved 2-5% not etc., and drip loss obviously reduces.
Related raw material is prior art and can purchases available conventional raw material and bacterial classification above. In addition should be understood that and readingAfter foregoing of the present invention, those skilled in the art can make various changes or modifications the present invention, these equivalent form of valuesFall within equally limited range of the present invention.
Claims (4)
1. active pig's feed additive of antibacterial peptide and preparation method thereof, it is characterized in that, formed by component A and B component, wherein component A is by corn flour 300-500 weight portion, pomace 20-30 weight portion, DDGS10-15 weight portion, corn protein powder 20-30 weight portion and dregs of beans 100-200 weight portion are prepared from through the fermentation of composite fermentation microbial inoculum; B component is by dark plum 8-10 weight portion, Herba Andrographitis 12-18 weight portion, and bighead atractylodes rhizome 3-5 weight portion, moutan bark 5-7 weight portion, Chinese prickly ash 1 weight portion, rice bran 50-80 weight portion is prepared from; Wherein said composite bacteria agent capable is bacillus licheniformis, bacillus pumilus and candida utili composition;
The preparation method who wherein prepares component A comprises the steps:
Step (1), takes corn flour, pomace, DDGS, corn protein powder and dregs of beans by weight, pulverizes 10-30 mesh sieve after being mixed, and then adds the pure water of mixture weight 30%, stirs;
Step (2), stir in the mixture obtaining and add the composite bacteria agent capable after activation to step (1), continue to stir, then at about 27-29 DEG C fermentation 8-12 hour, in this process in mixture by air, throughput be 15L air/100kg forage mixture/minute; Then adjust the temperature of fermenting mixture, at about 35-38 DEG C fermentation 8-12 hour, in this process in mixture by air, throughput be 5L air/100kg forage mixture/minute; Next adjust the temperature of fermentate, at 42-44 DEG C of left and right standing for fermentation 12-24 hour;
Step (3), the tunning that step (2) is made carries out low temperature drying, then is ground into powder, crosses 30-50 mesh sieve, obtains component A;
Wherein the preparation method of B component comprises the steps:
Step (one), takes dark plum, Herba Andrographitis, the bighead atractylodes rhizome, moutan bark, Chinese prickly ash and rice bran by weight, and the moutan bark taking and zanthoxylum powder are broken into 10-20 object powder, and the bran powder taking is broken into 50-60 object powder;
Step (two), gets the moutan bark and the Chinese prickly ash that take, the ethanol that is 75% by itself and volumetric concentration by etc. mass mixing, in closed container, be heated to 65-72 DEG C of left and right, extract 3-5 hour, then filter and obtain alcohol extracting filter residue and alcohol extracting filtrate; Alcohol extracting filter residue and the dark plum taking, Herba Andrographitis and the bighead atractylodes rhizome are mixed, add its mixture quality 1.5-2 water doubly, in closed container, be heated to 55-60 DEG C of left and right, extract 8-12 hour, then filter and obtain water extraction filtrate;
Step (three), the bran powder that the alcohol extracting filtrate that step (two) is made and water extraction filtrate and step () make mixes, and carries out low temperature drying, then be ground into powder after stirring, and crosses 30-50 mesh sieve, obtains B component.
2. active pig's feed additive of antibacterial peptide according to claim 1 and preparation method thereof, it is characterized in that, component A is by corn flour 400 weight portions, pomace 25 weight portions, DDGS15 weight portion, corn protein powder 25 weight portions and dregs of beans 150 weight portions are prepared from through the fermentation of composite fermentation microbial inoculum.
3. active pig's feed additive of antibacterial peptide according to claim 3 and preparation method thereof, is characterized in that, B component is by dark plum 9 weight portions, Herba Andrographitis 15 weight portions, the bighead atractylodes rhizome 4 weight portions, moutan bark 6 weight portions, Chinese prickly ash 1 weight portion, rice bran 80 weight portions are prepared from.
4. the active pig's feed additive of antibacterial peptide claimed in claim 1 is in the application of preparing in pig feed, after active described antibacterial peptide pig's feed additive is mixed according to the mass ratio of 1:50 with conventional pig feed raw material, use according to conventional feeding method.
Priority Applications (1)
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