CN105410448A - Antibacterial peptide active chicken feed additive and preparation method thereof - Google Patents
Antibacterial peptide active chicken feed additive and preparation method thereof Download PDFInfo
- Publication number
- CN105410448A CN105410448A CN201511019405.0A CN201511019405A CN105410448A CN 105410448 A CN105410448 A CN 105410448A CN 201511019405 A CN201511019405 A CN 201511019405A CN 105410448 A CN105410448 A CN 105410448A
- Authority
- CN
- China
- Prior art keywords
- weight portion
- component
- parts
- mixture
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to an antibacterial peptide active chicken feed additive and a preparation method thereof. The feed additive is composed of a component A and a component B. The component A is composed of the following components in parts by weight: 300 to 500 parts of corn flour, 20 to 30 parts of apple residue, 10 to 15 parts of DDGS, 20 to 30 parts of corn protein powder, and 100 to 200 parts of soybean meal, and is prepared by fermenting the components mentioned above by a composite fermentation inoculum. The component B is prepared from the following components in parts by weight: 8 to 10 parts of garden burnet, 12 to 18 parts of isatis root, 8 to 12 parts of cordate houttuynia, 3 to 5 parts of gelsemium elegans, 5 to 7 parts of calendula officinalis, 1 part of radix bupleuri, and 50 to 80 parts of rice bran. The feed additive is prepared from natural raw materials through fermentation and extraction, and thus is a natural, green, and safe feed additive.
Description
Technical field
The invention belongs to field of fodder, be specifically related to the preparation method of the active additive agent for chicken feed of a kind of antibacterial peptide.
Background technology
At present, along with the raising of people's living standard, the demand of chicken is increased day by day, also promote the development of poultry industry.Because chicken, duck etc. belong to bird, liftoff life distance is near, and the probability of contact bacterium, virus etc. wants high, and the immunocompetence of poultry is well below mammal, therefore, bird more easily falls ill, in the face of poultry disease day by day occurred frequently, antibiotic becomes treatment and prophylactic first-selection.Because antibiotic feed additive can improve animal productiong efficiency, be widely used in edible animal is raised.1996, the consumption of whole world antibiotic feed additive accounted for 45.8% of whole feed addictive consumption, antibiotics production total output about 50% for animal husbandry.But, because continuous low-level is fed antibiotic, the harm such as the bacterial drug resistance that antibiotic feed additive causes, livestock products medicament residue, irritated toxic reaction and " three cause " effect are day by day serious, are more and more subject to the concern of people and the restriction of many countries.From status quo and development trend, in edible animal forbid, limit the use of antibiotic feed additive become the world common recognition, be trend of the times.
At present, the alternative of fodder antibiotics mainly contains two kinds, and one improves the ratio of enteric microorganism for feed microbe additive and probiotic (bacterium), suppresses harmful microorganism, promotes growth of animal; Its two for use feeder acidulant, improve the acid-base value of intestinal contents, suppress harmful bacteria, promote beneficial bacterium growth.But in general, above two kinds of measures still have a certain distance relative to antibiotic effect.Various animal has established the microorganism species symbiosis ecosystem of balance in vivo in long-term natural evolution process, and establish the mechanism of flora stable state in its body of a set of maintenance, antibacterial peptide is that one of Main Factors in this mechanism plays very important effect in maintenance normal intestinal flora balance, and may have certain relation with Intestinal Mucosal Immunization.Compared with antibiotic, the bacteriostatic activity of antibacterial peptide is higher, scope of restraining fungi is wider, and the production performance for animal also has obvious facilitation; What is more important, antibacterial peptide can not produce any drug resistance; Therefore, Study and Development goes out suitable antibacterial peptide, will be the best substitute of fodder antibiotics.
Antibacterial peptide refers to be present in organism can resist external microbe infringement, and eliminate the small molecule polypeptide of vivo mutations cell, it is the important component part of innate immune system.Antibacterial peptide is made up of 15-45 amino acid residue usually, and major part is positively charged.Multiple biology is as all contained antibacterial peptide in bacterium, insect, plant and vertebrate.Due to becoming increasingly conspicuous of antibiotic resistance and residue problem, as the antibiotic substitute of one, antibacterial peptide receives much concern because it does not have antibiotic above-mentioned shortcoming.Antibiotic is applied for a long time in husbandry sector, but its Long-Time Service also result in drug resistance and the serious problems such as residual.Therefore be necessary that searching is similar to effects of antibiotics, and there is no the antibiotic substitute of the problems referred to above.Antibacterial peptide is a kind of antibacterial material extensively existed in vivo, is a kind of low-molecular-weight polypeptide, all has stronger lethal effect to Gram-positive and negative bacterium.
Although the useful effect of antibacterial peptide has obtained general confirmation, also in the industry cycle in obtain accreditation, but in recent years, the application of antibacterial peptide is still subject to larger limitation, specifically, on the one hand, its production technology is immature, and superior strain is difficult to find and industrialization production, on the other hand, with high costs, be difficult to promote on a large scale.Based on this, our company is through studying with domestic many well-known colleges and universities Long-term Collaboration, on the basis of great many of experiments, obtain a kind of preparation method of antibacterial peptide activated feed, such as, and specifically have studied various object of feeding, the growth of pig, chicken, duck, ox and aquatic livestock and feeding habits, carried out the further investigation of differentiation, obtain the preparation method of multiple concrete activated peptide forage, the preparation method of the active additive agent for chicken feed of antibacterial peptide such as involved in the present invention.
Summary of the invention
Be difficult to industrialization and the high defect of production cost to make up antibacterial peptide Feed Manufacturing in prior art, the present invention aims to provide the preparation method of the active additive agent for chicken feed of a kind of antibacterial peptide.
For overcoming the above problems, the present invention adopts following technical scheme:
Active additive agent for chicken feed of a kind of antibacterial peptide and preparation method thereof, it is characterized in that, be made up of component A and B component, wherein component A is by corn flour 300-500 weight portion, pomace 20-30 weight portion, DDGS10-15 weight portion, corn protein powder 20-30 weight portion and dregs of beans 100-200 weight portion form through composite fermentation microbial inoculum fermentation is standby; B component is by garden burnet 8-10 weight portion, Radix Isatidis 12-18 weight portion, cordate houttuynia 8-12 weight portion, elegant jessamine 3-5 weight portion, pot marigold 5-7 weight portion, radix bupleuri 1 weight portion, and rice bran 50-80 weight portion is prepared from; Wherein said composite bacteria agent capable is bacillus licheniformis (Bacilluslichnifarimis), bacillus pumilus (Bacillusbrevis) and candida utili (Candidautilis) composition.
The preparation method wherein preparing component A comprises the steps:
Step (1), takes corn flour, pomace, DDGS, corn protein powder and dregs of beans by weight, pulverizes 10-30 mesh sieve after being mixed, and then adds the pure water of mixture weight 30%, stirs;
Step (2), stir in the mixture obtained to step (1) and add the composite bacteria agent capable after activation, continue to stir, then at 27-29 DEG C of fermentation 8-12 hour, in the process in mixture by air, throughput be 15L air/100kg forage mixture/minute; Then adjust the temperature of fermenting mixture, at 35-38 DEG C of fermentation 8-12 hour, in the process in mixture by air, throughput be 5L air/100kg forage mixture/minute; The temperature of following adjustment fermentate, at 42-44 DEG C of standing for fermentation 12-24 hour;
Step (3), the tunning that step (2) is obtained carries out low temperature drying, then is ground into powder, crosses 30-50 mesh sieve, obtains component A.
Wherein the preparation method of B component comprises the steps:
Step (one), takes garden burnet, Radix Isatidis, cordate houttuynia, elegant jessamine, pot marigold, radix bupleuri and rice bran by weight, and the pot marigold taken and radix bupleuri are ground into 10-20 object powder, the bran powder taken is broken into 50-60 object powder;
Step (two), gets the pot marigold and radix bupleuri that take, by itself and volumetric concentration be 75% ethanol by etc. mass mixing, in closed container, be heated to 65-72 DEG C, extract 3-5 hour, then filter obtain alcohol extracting filter residue and alcohol extracting filtrate; By alcohol extracting filter residue and the garden burnet taken, Radix Isatidis, cordate houttuynia and elegant jessamine mixing, add its mixture quality 1.5-2 water doubly, in closed container, be heated to 55-60 DEG C, extract 8-12 hour, then filter and obtain water extraction filtrate;
Step (three), by alcohol extracting filtrate obtained for step (two) and water extraction filtrate and the obtained bran powder mixing of step (), carries out low temperature drying after stirring, then is ground into powder, cross 30-50 mesh sieve, obtain B component.
In process of production, obtained component A and B component can be mixed rear packaging and sell, also can be pack sale separately, mix when user's actual use.
Preferably, component A is by corn flour 400 weight portion, pomace 25 weight portion, DDGS15 weight portion, and corn protein powder 25 weight portion and dregs of beans 150 weight portion form through composite fermentation microbial inoculum fermentation is standby.
Preferably, B component is by garden burnet 9 weight portion, Radix Isatidis 15 weight portion, cordate houttuynia 10 weight portion, elegant jessamine 4 weight portion, pot marigold 6 weight portion, radix bupleuri 1 weight portion, and rice bran 80 weight portion is prepared from.
Preferably, as suggestion, but forcibly, not described composite bacteria agent capable is bacillus licheniformis (Bacilluslichnifarimis), bacillus pumilus (Bacilluspumilus) and candida utili (Candidautilis) to be formed according to viable count (CFU) 1:1:1.
Preferably, as suggestion, but not forcibly, described bacillus licheniformis is being numbered CGMCC1.1858 or being numbered the bacillus licheniformis of CGMCC1.7461 purchased from China General Microbiological culture presevation administrative center; Described bacillus pumilus is being numbered CGMCC1.3533 or being numbered the bacillus pumilus of CGMCC1.2160 purchased from China General Microbiological culture presevation administrative center, and described candida utili is being numbered CGMCC2.1180 or being numbered the candida utili of CGMCC2.1005 purchased from China General Microbiological culture presevation administrative center.
The using method of the active additive agent for chicken feed of antibacterial peptide involved in the present invention is: after it being mixed according to the mass ratio of 1:45 with conventional feedstuff, feeding method conveniently uses; Or after being mixed according to the mass ratio of 1:15 with water by feed addictive of the present invention, the routine of alternative chicken is drunk and is used water.
Based on above summary of the invention, tool of the present invention has the following advantages:
First, inventor is through repeatedly practising, draw the combination of the antibacterial peptide fermentation substrate formula that ferment effect is best and composite fermentation microbial inoculum, research shows, adopt composite fermentation microbial inoculum of the present invention only could obtain best ferment effect on fermentation substrate of the present invention, antibacterial effect is best, if by itself and conventional feedstuff mixed culture fermentation, the output of antibacterial peptide is extremely low.And, also a large amount of painstaking effort of inventor have been poured into during the fermentation, based on nutritional characteristic and the growth feature of each fermentation stage, finally draw stepwise fermentation mode involved in the present invention, the expansion relating generally to the basic metabolism of bacterial classification and bacterial classification in first stage of fermentation and second stage is numerous, second and three the stage relate generally to the accumulation of antibacterial peptide, the particularly fermentation of phase III, wherein saccharomycetic fermentation also creates certain similar fruital taste, also final tunning and component A is made to have good local flavor, the cooperation of this fermentation substrate and composite bacteria agent capable and fermentation processes are observant, also be applicant pour into the achievement in research of a large amount of painstaking effort, do not have in report in the past yet.
Second, the present invention has growth characteristic and the susceptible illness of chicken, according to the mechanism of Chinese medicine of veterinary medicine, with reference to traditional Chinese medical science tradition, have selected record medicine to combine, garden burnet wherein, Radix Isatidis and cordate houttuynia are as monarch drug in a prescription, mainly play the effect of sterilization, elegant jessamine is minister, pot marigold is assistant, radix bupleuri is for drawing, above medicine uses according to proportioning of the present invention, reach the synergy of 1+1>2, its resistance against diseases for chicken and immunity have had great lifting, particularly in the process of feeding, stop the problem of chicken diarrhea and dysentery completely, the speed of growth of chicken is also more traditional feeds or the fast growth of chicken that normal diet is fed, and meat is better.Illustratively, garden burnet selected in the present invention has function that is clearing heat and detoxicating, cooling blood and hemostasis, there is inhibitory action to staphylococcus aureus, Pseudomonas aeruginosa, also have inhibitory action to diphtheria, dysentery, large intestine, typhoid fever and paratyphosum Bacterium, also effective to influenza A virus; Radix Isatidis, has the function of clearing heat and detoxicating, cool blood, and its water logging agent has inhibitory action to staphylococcus aureus, Escherichia coli, shigella dysenteriae, also has inhibitory action to virus; Cordate houttuynia, has effect that is clearing heat and detoxicating, inducing diuresis to reduce edema, has significant inhibitory action, also have inhibitory action to Flu-A to micrococcus catarrhalis, pneumococcus, staphylococcus aureus; Elegant jessamine, has the effect of dispeling the wind, attacking poison, detumescence, pain relieving; Pot marigold, has the function of cooling blood and hemostasis, the promoting flow of qi and blood circulation, has calm effect, has stronger inhibitory action to staphylococcus, streptococcus etc.; Radix bupleuri, in having and inducing sweat, the soothing the liver function rising sun, have significantly antipyretic, calm, ease pain, antiinflammatory action.Above medicine is not simply had an effect separately in the present invention, but synergy.
3rd, antibacterial peptide feed addictive and Chinese medicine additive are combinationally used in the present invention, also serve good cooperative effect, its antibacterial, anti-infection effect reached is far longer than expection, and it also serves the effect of fabulous promotion growth of meat chicken.
In sum, the selected natural material of feed addictive of the present invention, obtained a kind of natural, green, safe feed addictive through fermentation or extraction, it compares traditional antibiotic medicine, more meets the demand of Modern Animal Husbandry development, and its production technology is simple, do not need complicated equipment, production cost is low, but it is effective, instant effect, has great productive value.Through pilot plant test, adopt feed addictive of the present invention only to increase by the cost of feeding of 1.5%, but the direct economic benefit of cultivation can improve about 20-25%, thus, feed addictive of the present invention has great social value and large market prospect.
Detailed description of the invention
Embodiment 1: the active additive agent for chicken feed of a kind of antibacterial peptide, be made up of component A and B component, wherein component A is by corn flour 400 weight portion, pomace 25 weight portion, DDGS15 weight portion, corn protein powder 25 weight portion and dregs of beans 150 weight portion form through composite fermentation microbial inoculum fermentation is standby; B component is by garden burnet 9 weight portion, Radix Isatidis 15 weight portion, cordate houttuynia 10 parts, elegant jessamine 4 weight portion, pot marigold 6 weight portion, radix bupleuri 1 weight portion, and rice bran 80 weight portion is prepared from; Wherein said composite bacteria agent capable is bacillus licheniformis (Bacilluslichnifarimis), bacillus pumilus (Bacillusbrevis) and candida utili (Candidautilis) composition.
The preparation method wherein preparing component A comprises the steps:
Step (1), takes corn flour, pomace, DDGS, corn protein powder and dregs of beans by weight, pulverizes 30 mesh sieves after being mixed, and then adds the pure water of mixture weight 30%, stirs;
Step (2), stir in the mixture obtained to step (1) and add the composite bacteria agent capable after activation, continue to stir, then 28 DEG C of fermentations 12 hours, in the process in mixture by air, throughput be 15L air/100kg forage mixture/minute; Then adjust the temperature of fermenting mixture, 35 DEG C of fermentations 8 hours, in the process in mixture by air, throughput be 5L air/100kg forage mixture/minute; The temperature of following adjustment fermentate, 44 DEG C of standing for fermentation 12 hours;
Step (3), the tunning that step (2) is obtained carries out low temperature drying, then is ground into powder, crosses 50 mesh sieves, obtains component A.
Wherein the preparation method of B component comprises the steps:
Step (one), takes garden burnet, Radix Isatidis, cordate houttuynia, elegant jessamine, pot marigold, radix bupleuri and rice bran by weight, and the pot marigold taken and radix bupleuri are ground into 10 object powder, the bran powder taken is broken into 50 object powder;
Step (two), gets the pot marigold and radix bupleuri that take, by itself and volumetric concentration be 75% ethanol by etc. mass mixing, in closed container, be heated to 65 DEG C, extract 3 hours, then filter obtain alcohol extracting filter residue and alcohol extracting filtrate; By alcohol extracting filter residue and the garden burnet taken, Radix Isatidis, cordate houttuynia and elegant jessamine mixing, add the water of its mixture quality 1.5 times, in closed container, be heated to 60 DEG C, extract 8 hours, then filter and obtain water extraction filtrate;
Step (three), by alcohol extracting filtrate obtained for step (two) and water extraction filtrate and the obtained bran powder mixing of step (), carries out low temperature drying after stirring, then is ground into powder, cross 50 mesh sieves, obtain B component.
Component A and B is mixed the i.e. active additive agent for chicken feed of obtained described antibacterial peptide.
Embodiment 2: the active additive agent for chicken feed of a kind of antibacterial peptide, it is characterized in that, be made up of component A and B component, wherein component A is by corn flour 500 weight portion, pomace 20 weight portion, DDGS15 weight portion, corn protein powder 20 weight portion and dregs of beans 100 weight portion form through composite fermentation microbial inoculum fermentation is standby; B component is by garden burnet 10 weight portion, Radix Isatidis 12 weight portion, cordate houttuynia 9 weight portion, elegant jessamine 3 weight portion, pot marigold 7 weight portion, radix bupleuri 1 weight portion, and rice bran 80 weight portion is prepared from; Wherein said composite bacteria agent capable is bacillus licheniformis (Bacilluslichnifarimis), bacillus pumilus (Bacillusbrevis) and candida utili (Candidautilis) composition.
The preparation method wherein preparing component A comprises the steps:
Step (1), takes corn flour, pomace, DDGS, corn protein powder and dregs of beans by weight, pulverizes 20 mesh sieves after being mixed, and then adds the pure water of mixture weight 30%, stirs;
Step (2), stir in the mixture obtained to step (1) and add the composite bacteria agent capable after activation, continue to stir, then 27 DEG C of fermentations 12 hours, in the process in mixture by air, throughput be 15L air/100kg forage mixture/minute; Then adjust the temperature of fermenting mixture, 38 DEG C of fermentations 8 hours, in the process in mixture by air, throughput be 5L air/100kg forage mixture/minute; The temperature of following adjustment fermentate, 42 DEG C of standing for fermentation 24 hours;
Step (3), the tunning that step (2) is obtained carries out low temperature drying, then is ground into powder, crosses 50 mesh sieves, obtains component A.
Wherein the preparation method of B component comprises the steps:
Step (one), takes garden burnet, Radix Isatidis, cordate houttuynia, elegant jessamine, pot marigold, radix bupleuri and rice bran by weight, and the pot marigold taken and radix bupleuri are ground into 20 object powder, the bran powder taken is broken into 50 object powder;
Step (two), gets the pot marigold and radix bupleuri that take, by itself and volumetric concentration be 75% ethanol by etc. mass mixing, in closed container, be heated to 72 DEG C, extract 3 hours, then filter obtain alcohol extracting filter residue and alcohol extracting filtrate; By alcohol extracting filter residue and the garden burnet taken, Radix Isatidis, cordate houttuynia and elegant jessamine mixing, add the water of its mixture quality 2 times, in closed container, be heated to 55 DEG C, extract 12 hours, then filter and obtain water extraction filtrate;
Step (three), by alcohol extracting filtrate obtained for step (two) and water extraction filtrate and the obtained bran powder mixing of step (), carries out low temperature drying after stirring, then is ground into powder, cross 50 mesh sieves, obtain B component.
Component A and B is mixed the i.e. active additive agent for chicken feed of obtained described antibacterial peptide.
Embodiment 3: antibacterial, bacteriostatic experiment
In order to verify the antibacterial activity of product of the present invention, spy arranges following three contrast test groups, wherein:
Check experiment group 1 is by corn flour 400 weight portion, pomace 25 weight portion, DDGS15 weight portion, and corn protein powder 25 weight portion and dregs of beans 150 weight portion form through composite fermentation microbial inoculum fermentation is standby; Wherein said composite bacteria agent capable is bacillus licheniformis (Bacilluslichnifarimis), bacillus pumilus (Bacillusbrevis) and candida utili (Candidautilis) composition.Its preparation method comprises the steps: step (1), take corn flour, pomace, DDGS, corn protein powder and dregs of beans by weight, pulverized 30 mesh sieves after being mixed, and then added the pure water of mixture weight 30%, stir; Step (2), stir in the mixture obtained to step (1) and add the composite bacteria agent capable after activation, continue to stir, then 32 DEG C of fermentations 48 hours, in the process in mixture by air, throughput be 15L air/100kg forage mixture/minute; Step (3), the tunning that step (2) is obtained carries out low temperature drying, then is ground into powder, crosses 50 mesh sieves, obtains the product of check experiment group 1.
Check experiment group 2 is by corn flour 400 weight portion, and corn protein powder 25 weight portion and dregs of beans 150 weight portion form through composite fermentation microbial inoculum fermentation is standby; Wherein said composite bacteria agent capable is bacillus licheniformis (Bacilluslichnifarimis), bacillus pumilus (Bacillusbrevis) and candida utili (Candidautilis) composition.Its preparation method comprises the steps: step (1), takes corn flour, corn protein powder and dregs of beans by weight, pulverizes 30 mesh sieves after being mixed, and then adds the pure water of mixture weight 30%, stirs; Step (2), stir in the mixture obtained to step (1) and add the composite bacteria agent capable after activation, continue to stir, then 29 DEG C of fermentations 8 hours, in the process in mixture by air, throughput be 15L air/100kg forage mixture/minute; Then adjust the temperature of fermenting mixture, 38 DEG C of fermentations 8 hours, in the process in mixture by air, throughput be 5L air/100kg forage mixture/minute; The temperature of following adjustment fermentate, 44 DEG C of standing for fermentation 12 hours; Step (3), the tunning that step (2) is obtained carries out low temperature drying, then is ground into powder, crosses 50 mesh sieves, obtains the product of check experiment group 2.
Check experiment group 3 is the component A of the embodiment of the present invention 1.
Two test group are set, be respectively the product of the embodiment of the present invention 1,2, the product of water intaking normal temperature lixiviate embodiment 1,2 and check experiment group 1-3 respectively, the ratio of product and water is 1:10, normal temperature extracts 5 hours, after then leaching liquor being filtered, filtrate is crossed the filter of 0.22 micron, obtain aseptic extraction filtrate, get aseptic extraction filtrate and respectively inhibition zone test is carried out to staphylococcus aureus, Escherichia coli, salmonella and Shigella, the size of record inhibition zone, concrete outcome sees the following form 1:
Table 1 bacteriostatic test result-inhibition zone size (mm)
Can by above bacteriostatic test, the embodiment of the present invention 1, the product of 2 is for common livestock and poultry, aquatic products susceptible germ-staphylococcus aureus, Escherichia coli, salmonella and Shigella all have good inhibitory action, and more known by check experiment group 3 and check experiment group 1 and 2, in the preparation process of instant component A, fermentation substrate and fermentation parameter have important impact for the output of tunning-antibacterial peptide and antibacterial activity, departing under working condition of the present invention, its antibacterial effect obviously weakens, and comparing from embodiment 1 and 2 and contrast test group 3, in product of the present invention, B component and component A's is significantly collaborative with the use of having for bacteriostatic activity, the effect strengthened, its bacteriostatic level is significantly better than being used alone component A.
Embodiment 4: animal feeding trials
Choose Broiler chicks 500 plumage that body weight is 0.5-0.75kg, be divided into 5 groups at random, wherein organizing 1 is blank group, adopts common commercially available broiler fodder to feed; Group 2-3 is experimental group, feeds after adopting the active additive agent for chicken feed of the antibacterial peptide of embodiment 1,2 to mix according to the mass ratio of 1:45 with common commercially available broiler fodder respectively; Group 4-5 is contrast groups, component A respectively in employing embodiment 1, B component are fed after mixing according to the mass ratio of 1:45 with common commercially available broiler fodder, feed to broiler chicken and deliver for sale, statistics infection morbidity number of times (heating, diarrhoea etc. infect disease summation, and 1 plumage chicken falls ill and counts 1 time 1 time), the death rate and average weight growth rate (average weight recruitment/initial average weight * 100%).Concrete outcome sees the following form:
| Infection morbidity number of times | The death rate | Average weight growth rate | |
| Group 1 (blank) | 531 | 12％ | 210.5％ |
| Group 2 (embodiments 1) | 68 | 0％ | 343.2％ |
| Group 3 (embodiments 2) | 76 | 1％ | 335.4％ |
| Group 4 (component A) | 497 | 8％ | 221.5％ |
| Group 5 (B component) | 455 | 9％ | 239.6％ |
From the result of upper table, compare and do not add additivated situation, add additive of the present invention or the obtained component A of embodiment 1 or B component and all achieve the effect improving chicken anti-infection ability, and growing state is also had some improvement, but, compare group 4 and group 5, the culture efficiency of group 2-3 obviously will be got well, it infects frequency and is obviously less than feed separately component A or B component, the death rate will lower than component A or the B component of feeding separately, and be better than the two simple superposition, illustrate that component A and B component serve the effect of Synergistic.
Embodiment 5: actually to feed
Have chosen the 5 family raisers in area, Jiangsu and Zhejiang Provinces, product of the present invention on probation, feed from the Broiler chicks of 0.5kg until deliver for sale, feed back through raiser, product of the present invention significantly can reduce the generation of chicken disease, the particularly generation of communicable disease, decrease the use of conventional medicine and antibiotic medicine, the medicine cost and human cost of feeding are saved, simultaneously, the average weight of delivering for sale has had certain lifting, and do not use or use other additives before comparing, the economic benefit of raising improves about 20%-25%.
Raw material involved is above prior art and can purchases available convenient source and bacterial classification.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read foregoing of the present invention, these equivalent form of values fall within limited range of the present invention equally.
Claims (4)
1. active additive agent for chicken feed of antibacterial peptide and preparation method thereof, it is characterized in that, be made up of component A and B component, wherein component A is by corn flour 300-500 weight portion, pomace 20-30 weight portion, DDGS10-15 weight portion, corn protein powder 20-30 weight portion and dregs of beans 100-200 weight portion form through composite fermentation microbial inoculum fermentation is standby; B component is by garden burnet 8-10 weight portion, Radix Isatidis 12-18 weight portion, cordate houttuynia 8-12 weight portion, elegant jessamine 3-5 weight portion, pot marigold 5-7 weight portion, radix bupleuri 1 weight portion, and rice bran 50-80 weight portion is prepared from; Wherein said composite bacteria agent capable is bacillus licheniformis (Bacilluslichnifarimis), bacillus pumilus (Bacillusbrevis) and candida utili (Candidautilis) composition;
The preparation method wherein preparing component A comprises the steps:
Step (1), takes corn flour, pomace, DDGS, corn protein powder and dregs of beans by weight, pulverizes 10-30 mesh sieve after being mixed, and then adds the pure water of mixture weight 30%, stirs;
Step (2), stir in the mixture obtained to step (1) and add the composite bacteria agent capable after activation, continue to stir, then at about 27-29 DEG C fermentation 8-12 hour, in the process in mixture by air, throughput be 15L air/100kg forage mixture/minute; Then adjust the temperature of fermenting mixture, at about 35-38 DEG C fermentation 8-12 hour, in the process in mixture by air, throughput be 5L air/100kg forage mixture/minute; The temperature of following adjustment fermentate, at about 42-44 DEG C standing for fermentation 12-24 hour;
Step (3), the tunning that step (2) is obtained carries out low temperature drying, then is ground into powder, crosses 30-50 mesh sieve, obtains component A;
Wherein the preparation method of B component comprises the steps:
Step (one), takes garden burnet, Radix Isatidis, cordate houttuynia, elegant jessamine, pot marigold, radix bupleuri and rice bran by weight, and the pot marigold taken and radix bupleuri are ground into 10-20 object powder, the bran powder taken is broken into 50-60 object powder;
Step (two), gets the pot marigold and radix bupleuri that take, by itself and volumetric concentration be 75% ethanol by etc. mass mixing, in closed container, be heated to about 65-72 DEG C, extract 3-5 hour, then filter obtain alcohol extracting filter residue and alcohol extracting filtrate; By alcohol extracting filter residue and the garden burnet taken, Radix Isatidis, cordate houttuynia and elegant jessamine mixing, add its mixture quality 1.5-2 water doubly, in closed container, be heated to about 55-60 DEG C, extract 8-12 hour, then filter and obtain water extraction filtrate;
Step (three), by alcohol extracting filtrate obtained for step (two) and water extraction filtrate and the obtained bran powder mixing of step (), carries out low temperature drying after stirring, then is ground into powder, cross 30-50 mesh sieve, obtain B component.
2. active additive agent for chicken feed of antibacterial peptide according to claim 1 and preparation method thereof, it is characterized in that, component A is by corn flour 400 weight portion, pomace 25 weight portion, DDGS15 weight portion, corn protein powder 25 weight portion and dregs of beans 150 weight portion form through composite fermentation microbial inoculum fermentation is standby.
3. active additive agent for chicken feed of antibacterial peptide according to claim 3 and preparation method thereof, it is characterized in that, B component is by garden burnet 9 weight portion, Radix Isatidis 15 weight portion, cordate houttuynia 10 weight portion, elegant jessamine 4 weight portion, pot marigold 6 weight portion, radix bupleuri 1 weight portion, rice bran 80 weight portion is prepared from.
4. the application of the active additive agent for chicken feed of antibacterial peptide according to claim 1 in preparation chicken feed, after being mixed according to the mass ratio of 1:45 with conventional chicken feedstuff by active for described antibacterial peptide additive agent for chicken feed, feeding method conveniently uses.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511019405.0A CN105410448A (en) | 2015-12-30 | 2015-12-30 | Antibacterial peptide active chicken feed additive and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511019405.0A CN105410448A (en) | 2015-12-30 | 2015-12-30 | Antibacterial peptide active chicken feed additive and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105410448A true CN105410448A (en) | 2016-03-23 |
Family
ID=55489369
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511019405.0A Pending CN105410448A (en) | 2015-12-30 | 2015-12-30 | Antibacterial peptide active chicken feed additive and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105410448A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106260603A (en) * | 2016-05-14 | 2017-01-04 | 樊正梅 | A kind of processing method that pomace is modified as cake taste feed additive |
| CN106260582A (en) * | 2016-05-14 | 2017-01-04 | 樊正梅 | A kind of processing method that pomace is modified as peanut taste feed additive |
| CN108669356A (en) * | 2018-07-26 | 2018-10-19 | 石阡县毅峰农牧开发有限公司 | A kind of special Se-enriched feedstuff of chicken |
| CN108669355A (en) * | 2018-07-26 | 2018-10-19 | 石阡县毅峰农牧开发有限公司 | A kind of chicken finishing period feed and preparation method thereof |
| CN112006163A (en) * | 2020-09-29 | 2020-12-01 | 湖南芭颉生态农牧股份有限公司 | Fermented feed for chickens and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101904430A (en) * | 2009-06-05 | 2010-12-08 | 哈尔滨鼎汇饲料有限公司 | Feed for enhancing immunity of layer chicken and preparation method thereof |
| CN102630852A (en) * | 2012-05-14 | 2012-08-15 | 山东新希望六和集团有限公司 | Fermented broiler feed and preparation method thereof |
| CN104000005A (en) * | 2014-04-22 | 2014-08-27 | 淮北极速时代动物营养有限公司 | Antibacterial peptide live bacteria fermented feed and preparation method thereof |
| CN104982750A (en) * | 2015-08-06 | 2015-10-21 | 山东新希望六和集团有限公司 | Fermentation type broiler chicken mixed feed and preparing method thereof |
| CN105104772A (en) * | 2015-06-30 | 2015-12-02 | 河南双成生物科技有限公司 | Method for producing laying hen complete feed through solid enzymolysis fermentation |
-
2015
- 2015-12-30 CN CN201511019405.0A patent/CN105410448A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101904430A (en) * | 2009-06-05 | 2010-12-08 | 哈尔滨鼎汇饲料有限公司 | Feed for enhancing immunity of layer chicken and preparation method thereof |
| CN102630852A (en) * | 2012-05-14 | 2012-08-15 | 山东新希望六和集团有限公司 | Fermented broiler feed and preparation method thereof |
| CN104000005A (en) * | 2014-04-22 | 2014-08-27 | 淮北极速时代动物营养有限公司 | Antibacterial peptide live bacteria fermented feed and preparation method thereof |
| CN105104772A (en) * | 2015-06-30 | 2015-12-02 | 河南双成生物科技有限公司 | Method for producing laying hen complete feed through solid enzymolysis fermentation |
| CN104982750A (en) * | 2015-08-06 | 2015-10-21 | 山东新希望六和集团有限公司 | Fermentation type broiler chicken mixed feed and preparing method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王秀: ""中草药饲料添加剂在外向型养殖业中的应用"", 《当代畜禽养殖业》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106260603A (en) * | 2016-05-14 | 2017-01-04 | 樊正梅 | A kind of processing method that pomace is modified as cake taste feed additive |
| CN106260582A (en) * | 2016-05-14 | 2017-01-04 | 樊正梅 | A kind of processing method that pomace is modified as peanut taste feed additive |
| CN108669356A (en) * | 2018-07-26 | 2018-10-19 | 石阡县毅峰农牧开发有限公司 | A kind of special Se-enriched feedstuff of chicken |
| CN108669355A (en) * | 2018-07-26 | 2018-10-19 | 石阡县毅峰农牧开发有限公司 | A kind of chicken finishing period feed and preparation method thereof |
| CN108669355B (en) * | 2018-07-26 | 2021-12-31 | 石阡县毅峰农牧开发有限公司 | Feed for local chickens in growing period and preparation method thereof |
| CN112006163A (en) * | 2020-09-29 | 2020-12-01 | 湖南芭颉生态农牧股份有限公司 | Fermented feed for chickens and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105851492A (en) | Traditional Chinese medicine probiotic fermented feed and preparation method | |
| CN102657287B (en) | Compound feed addictive with bacteria and oxidation resisting functions | |
| CN102696884B (en) | Composite feed additive product | |
| CN105410448A (en) | Antibacterial peptide active chicken feed additive and preparation method thereof | |
| CN109362987B (en) | Poultry antibiotic-free mulberry leaf fermentation concentrated material and preparation method thereof | |
| CN103340300A (en) | Preparation method for green ecological crucian forage | |
| CN102422996B (en) | Preliminary microbial fermentation antibiotic-free feed for growth fattening pigs | |
| CN111011643A (en) | Bacterium enzyme fermented traditional Chinese medicine for improving growth performance of broiler chicken and preparation method thereof | |
| CN104247878A (en) | Compound pig feed capable of improving body immunity | |
| CN104543375A (en) | Compound fermented Chinese herbal medicine preparation for livestock and poultry and preparation method and applications thereof | |
| CN102669435A (en) | Feed additive product | |
| CN105360644A (en) | Fermented feedstuff preparation for piglets as well as preparation method and application thereof | |
| CN107853461A (en) | A kind of Rosa roxburghii slag fermentation nonreactive feed and preparation method thereof | |
| CN105360615A (en) | Fermented feed raw material preparation as well as preparation method and application thereof | |
| CN106260728A (en) | A kind of egg feedstuff improving resistance against diseases | |
| CN110292114A (en) | A kind of fermented feed that Yak Growth performance can be improved | |
| CN103829079A (en) | Green ecotype crucian carp feed | |
| CN104982665A (en) | Preparation method of probiotic fermentation cattle feed additive | |
| CN105124144A (en) | Antibiotic-free biological feed for livestock | |
| CN104686866A (en) | Decavitamin and fermented Chinese traditional medicinal herb preparation special for postpartum breeding sows and preparing method and application thereof | |
| CN102763772B (en) | Functional feed additive | |
| JP7042834B2 (en) | How to make fermented guar meal | |
| CN101874812B (en) | Composite bacteria preparation and application thereof in weight increment of immature livestock and control of diarrhea | |
| CN106721487A (en) | A kind of grouper fermentation of Chinese herbal medicine immunopotentiator | |
| CN105594993A (en) | Antibacterial active peptide feed additive for pigs and preparing method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20180614 Address after: 332200 Ruichang City, Jiujiang, Jiangxi Province, the South Garden of the Jing Kai District Applicant after: Jiangxi pastoral Wiley yuan Bio Technology Co., Ltd. Address before: 315043 003, 17-3 (17-4) 27, 3, 5, 6, century East Commercial Plaza, Jiangdong District, Ningbo, Zhejiang. Applicant before: Ningbo Jiangdong Nongru Biotechnology Co., Ltd. |
|
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20190104 Address after: 332200 Jiangxi Huamu Biotechnology Co., Ltd. Jingkai District, Ruichang City, Jiujiang City, Jiangxi Province Applicant after: Jiangxi Huamu Biotechnology Co., Ltd. Address before: 332200 Ruichang City, Jiujiang, Jiangxi Province, the South Garden of the Jing Kai District Applicant before: Jiangxi pastoral Wiley yuan Bio Technology Co., Ltd. |
|
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160323 |