CN102232467B - Livestock and poultry anti-diarrheal feed additive and preparation method thereof - Google Patents
Livestock and poultry anti-diarrheal feed additive and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a livestock and poultry anti-diarrheal feed additive and a preparation method thereof. In a technical scheme, the preparation method comprises the following steps of: smashing India quassiawood, dried ginger, licorice, costus root and the like; screening through a sieve; adding bean pulp, corn starch, bran and water to prepare a fermentation medium; adjusting the pH value, sterilizing, cooling, and inoculating for fermenting; and drying after the fermentation is finished to obtain the livestock and poultry anti-diarrheal feed additive. The livestock and poultry anti-diarrheal feed additive disclosed by the invention has the better functions of broad-spectrum bacterium resistance, virus resistance, parasite resistance, maintaining of the balance of microbial flora in the animal bodies, and can be used for effectively reducing the incidence of diarrheal of the livestock and poultry, promoting the growth of the livestock and poultry and reducing the cultivation investment; and the livestock and poultry anti-diarrheal feed additive has the characteristics of no toxic and side effects, no pollution, no residue, stable and reliable effect and the like, cannot give rise to drug tolerance, and can meet the requirement of people on safety production of the livestock and poultry products. The livestock and poultry anti-diarrheal feed additive disclosed by the invention also can be used as a veterinary drug in an increased dose manner, and has a remarkable curative effect for treating the diarrheal caused by various viruses, bacteria and parasitic infection for the livestock and poultry.
Description
Technical field
The invention belongs to feed additive field, relate to a kind of livestock and poultry anti diar rhea feed addictive and preparation method thereof.
Diarrhoea is a global problem, is perplexing for a long time the development of livestock and poultry breeding industry.This disease is popular wide, all the year round morbidity, even survive dead newborn animal or young fowl also often causes poor growth, the price of deed reduces, and causes heavy economic losses.Newborn animal (fowl) is not yet set up stable microflora because of enteron aisle, and self has a low resistance, and stimulates to external world responsively, is subject to various pathogenic microorganisms invasion and attack and various stress the impact and falls ill.
Diarrhoea not yet has effective immunization method at present, and drug therapy is still main means of prevention.For a long time, usually adopt antibacterial drug therapy on the veterinary clinic, but along with antibiotic prolonged application, produce antibody-resistant bacterium and reduced curative effect; Simultaneously a large amount of uses of these medicines cause the animal products drug residue, this serious threat mankind's health.
The purpose of this invention is to provide that a kind of effect stability is reliable, the livestock and poultry anti diar rhea feed addictive of instant effect, have antibiotic, antiviral, anti parasitic, keep in the animal body effect such as microorganism species balance, can effectively reduce the incidence of livestock and poultry diarrhea, promote growth, effectively solve prior art easily residual in the livestock and poultry body and develop immunity to drugs, bring the defectives such as hidden danger to human food security.
Another object of the present invention provides the preparation method of this livestock and poultry anti diar rhea feed addictive.
The present invention also provides a kind of feed that comprises this livestock and poultry anti diar rhea feed addictive.
To achieve these goals, the technical solution adopted in the present invention is as follows:
Described a kind of livestock and poultry anti diar rhea feed addictive, it is characterized in that the method that adopts Chinese medicine to pass through probiotics fermention obtains, wherein Chinese medicine comprises 1～10 part quassia, 1～5 part rhizoma zingiberis, 1～10 part Radix Glycyrrhizae, 1～10 part the banksia rose by weight, and probio is any one or more than one combination in saccharomycete, lactic acid bacteria, the Bacillus.
Described livestock and poultry anti diar rhea feed addictive is characterized in that described Chinese medicine comprises that also parts by weight are one or more in the granatum of 1～10 part Sophora alopecuroide, 1～10 part the Radix Astragali, 1～10 part the bark of eucommia, 1～10 part dandelion, 1～5 part kuh-seng, 1～5 part golden cypress, 1～5 part turmeric, 1～5 part akebi, 1～5 part the bighead atractylodes rhizome, 1～5 part Chinese ephedra, 1～5 part alum, 1～5 part medical stone and 1～5 part.
Described livestock and poultry anti diar rhea feed addictive is characterized in that described saccharomycete is candida utili, saccharomyces cerevisiae and/or brewer's yeast.
Described livestock and poultry anti diar rhea feed addictive is characterized in that described lactic acid bacteria comprises VREF, lactobacillus lactis, lactobacillus acidophilus, Lactobacillus casei, lactobacillus lactis and/or lactobacillus bulgaricus.
Described livestock and poultry anti diar rhea feed addictive is characterized in that described Bacillus comprises bacillus licheniformis, bacillus subtilis and/or bacillus cereus.
The preparation method of described a kind of livestock and poultry anti diar rhea feed addictive is characterized in that may further comprise the steps:
(1) Chinese medicine preliminary treatment
Choose Chinese medicine, behind 50～60 ℃ of lower oven dry 2～3h, pulverize, cross 80～120 mesh sieves, for subsequent use;
(2) preparation strain fermentating liquid
Each bacterial classification is inoculated in respectively in separately the optimum medium, cultivate under suitable condition primary seed solution, enlarge cultivation when each bacteria growing is transferred to respectively in the secondary seed fermentation tank during to exponential phase, stop when reaching exponential phase cultivating, for subsequent use;
(3) fermentation
Chinese medicine behind the crushing screening is mixed according to a certain ratio, pack in the fermentation tank, add the dregs of beans, 0～15% corn flour, 5～20% wheat brans that account for Chinese medicine gross weight 0～5%, add water to water content between 50～70%, behind immersion 12～24h, regulate between the pH value 6.0～7.5, at 121 ℃ of lower steam sterilizing 15～20min, after the cooling, add the total strain liquid that accounts for Chinese medicine gross weight 5%～20%, 28～37 ℃ fermented 36～96 hours, stirred once every 8h; After the fermentation ends, 35～50 ℃ of lower oven dry, namely get control diarrhoea feed addictive.
The preparation method of described livestock and poultry anti diar rhea feed addictive is characterized in that total strain liquid is that secondary seed solution by each bacterium is mixed and made into seed mixture liquid by equal proportion
Described a kind of feed that contains livestock and poultry anti diar rhea feed addictive is characterized in that, comprises the constituent of following part by weight: described livestock and poultry anti diar rhea feed addictive 0.02～0.2% and auxiliary material 99.98～99.8%.
The invention provides a kind of livestock and poultry anti diar rhea feed addictive and preparation method thereof, have the following advantages:
1, livestock and poultry anti diar rhea feed addictive of the present invention has preferably broad-spectrum antiseptic, antiviral, anti parasitic, keeps in the animal body effect such as microorganism species balance, can effectively reduce the incidence of livestock and poultry diarrhea, promotes growth, reduces cultivation to drop into;
2, livestock and poultry anti diar rhea feed addictive of the present invention is to adopt probio direct fermentation Chinese medicine, condition Wen Li, protected active ingredient of Chinese herbs to exempt from destruction, some active constituent content of possibility increases and produces new effective component simultaneously, and product can improve curative effect of medication, enlarges indication, reduce taking dose;
3, livestock and poultry anti diar rhea feed addictive of the present invention is to adopt probiotics fermention, can produce such as cellulase, amylase, protease etc., so that the substance of the difficult absorptions such as the cellulose in the Chinese medicine, lignin is fully degraded, Effective Component of Chinese Medicine is discharged to greatest extent, simultaneously cellulose conversion can be become compound sugar, promote the growth of beneficial bacterium in the animal gastrointestinal tract; Under the effect of the various enzymes that produce, the active ingredient in the feed is easier to be absorbed by animal simultaneously;
4, livestock and poultry anti diar rhea feed addictive of the present invention is a kind of novel fodder additive that utilizes the interaction development of Chinese medicine and probio, unrivaled advantage is arranged, and it has and has no side effect, pollution-free, noresidue, do not produce the security features such as drug resistance;
5, livestock and poultry anti diar rhea feed addictive instant effect of the present invention, effect stability are reliable, can satisfy people to the requirement of meat, egg, milk safety in production;
6, livestock and poultry anti diar rhea feed addictive of the present invention can also be used as veterinary drug by improving its using dosage, and the diarrhoea of preventing and the various viruses for the treatment of livestock and poultry, bacterium, parasitic infection cause is all had significant curative effect.
The specific embodiment
Embodiment 1
Raw material: 3 parts of rhizoma zingiberis, 2 parts of the banksia rose, 6 parts in Radix Glycyrrhizae, 3 parts of quassias
The preparation method:
(1) bacillus licheniformis is inoculated in the common nutrient broth medium, at 37 ℃, the 200rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into expansion cultivation in the secondary seed fermentation tank with inoculum concentration 5%, cultivation temperature is 30～32 ℃, and rotating speed is 200rpm, when bacterial content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
(2) S. cervisiae is inoculated in the malt extract medium, 28 ℃, the 200rpm shaking table is cultivated, and the production primary seed solution is when saccharomycete content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into expansion cultivation in the secondary seed fermentation tank with inoculum concentration 6%, cultivation temperature is 30～32 ℃, and rotating speed is 200rpm, when saccharomycete content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
(3) choose above-mentioned each raw material, behind 50～60 ℃ of lower oven dry 2～3h, pulverize, cross the 80-120 mesh sieve, for subsequent use;
(4) take by weighing raw material after the pulverizing by above-mentioned prescription, add and account for the dregs of beans of raw material gross weight 3%, 6% corn flour, 8% wheat bran, add water to water content at 50-60%, mix, at room temperature soak 24h, regulate about pH6.9, at 121 ℃ of lower steam sterilizing 20min, after the cooling, adding accounts for the Bacillus licheniformis liquid of raw material gross weight 5% and 5% S. cervisiae bacterium liquid respectively, at 30～32 ℃ of fermentation 36h, stir once every 8h; When total viable count reaches 1.0 * 10
9Stop fermentation during CFU/ml, 45 ℃ of lower vacuum drying make livestock and poultry anti diar rhea feed addictive.
Embodiment 2
Raw material: 5 parts of the banksia rose, 3 parts of rhizoma zingiberis, 8 parts in Radix Glycyrrhizae, 6 parts of quassias, 1 part of Sophora alopecuroide, 5 parts of the Radixs Astragali, 5 parts of the barks of eucommia, 3 parts of dandelions, 2 parts of kuh-sengs, 1 part in turmeric, 1 part of akebi, 1 part of the bighead atractylodes rhizome, 2 parts in alum.
The preparation method:
(1) bacillus subtilis is inoculated in the common nutrient broth medium, at 37 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into expansion cultivation in the secondary seed fermentation tank with inoculum concentration 3%, cultivation temperature is 35 ± 2 ℃, and rotating speed is 180rpm, when bacterial content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
(2) lactobacillus lactis and lactobacillus bulgaricus are inoculated into respectively in the MRS culture medium, 37 ℃, the 200rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into expansion cultivation in the secondary seed fermentation tank with inoculum concentration 5%, cultivation temperature is 35 ± 2 ℃, and rotating speed is 180rpm, when bacterial content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
(3) choose above-mentioned each raw material, behind 50～60 ℃ of lower oven dry 2～3h, pulverize, cross the 80-120 mesh sieve, for subsequent use;
(4) take by weighing raw material after the pulverizing by above-mentioned prescription, add and account for the dregs of beans of raw material gross weight 5%, 5% corn flour, 5% wheat bran, add water to water content toward 50～55%, mix, at room temperature soak 24h, regulate about pH6.6, at 121 ℃ of lower steam sterilizing 20min, after the cooling, add respectively the bacillus subtilis bacterium liquid account for raw material gross weight 5%, 5% lactobacillus lactis bacterium liquid and 5% lactobacillus bulgaricus bacterium liquid, at 35 ± 2 ℃ of fermentation 48h, stir once every 8h; When total viable count reaches 1.0 * 10
9Stop fermentation during CFU/ml, 40 ℃ of lower vacuum drying make livestock and poultry anti diar rhea feed addictive.
Embodiment 3
Raw material: 5 parts in Radix Glycyrrhizae, 3 parts of quassias, 10 parts of the banksia rose, 6 parts of rhizoma zingiberis, 2 parts of Sophora alopecuroides, 3 parts of the Radixs Astragali, 3 parts of the barks of eucommia, 4 parts of dandelions, 2 parts of golden cypresses, 1 part in alum, 2 parts in Chinese ephedra, 1 part of medical stone
The preparation method:
(1) bacillus licheniformis is inoculated in the common nutrient broth medium, at 37 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into expansion cultivation in the secondary seed fermentation tank with inoculum concentration 3%, cultivation temperature is 32～34 ℃, and rotating speed is 180rpm, when bacterial content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
(2) candida utili is inoculated in the malt extract medium, 28 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution is when saccharomycete content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into expansion cultivation in the secondary seed fermentation tank with inoculum concentration 5%, cultivation temperature is 32～34 ℃, and rotating speed is 180rpm, when saccharomycete content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
(3) VREF, Lactobacillus casei and lactobacillus bulgaricus are inoculated into respectively in the MRS culture medium, 37 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into expansion cultivation in the secondary seed fermentation tank with inoculum concentration 5%, cultivation temperature is 32～34 ℃, and rotating speed is 180rpm, when bacterial content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
(4) choose above-mentioned each raw material, behind 50～60 ℃ of lower oven dry 2～3h, pulverize, cross the 80-120 mesh sieve, for subsequent use;
(5) take by weighing material powder after the pulverizing by above-mentioned prescription, add the dregs of beans that accounts for raw material gross weight 2%, 5% corn flour, 15% wheat bran, add water to water content 55～60%, mix, at room temperature soak 24h, regulate about pH6.9, at 121 ℃ of lower steam sterilizing 20min, after the cooling, add respectively the Bacillus licheniformis liquid that accounts for raw material gross weight 2%, 2% candida utili bacterium bacterium liquid, the lactobacillus bulgaricus bacterium liquid of 2% VREF bacterium liquid and 2% Lactobacillus casei bacterium liquid and 2%, at 32～34 ℃ of fermentation 60h, stir once every 8h; When total viable count reaches 1.0 * 10
9Stop fermentation during CFU/ml, 50 ℃ of lower vacuum drying make livestock and poultry anti diar rhea feed addictive.
Embodiment 4
Raw material: 10 parts in Radix Glycyrrhizae, 8 parts of quassias, 6 parts of the banksia rose, 5 parts of rhizoma zingiberis, 1 part of Sophora alopecuroide, 8 parts of the Radixs Astragali, 10 parts of the barks of eucommia, 3 parts of dandelions, 1 part of golden cypress, 3 parts of quassias, 3 parts of kuh-sengs, 1 part of rhizoma zingiberis, 1 part in alum, 2 parts in Chinese ephedra, 1 part of medical stone, 2 parts of preparation methods of granatum:
(1) candida utili, brewer's yeast and saccharomyces cerevisiae are inoculated into respectively in the malt extract medium, 28～30 ℃, the 220rpm shaking table is cultivated, and the production primary seed solution is when saccharomycete content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into expansion cultivation in the secondary seed fermentation tank with inoculum concentration 5%, cultivation temperature is 28～30 ℃, and rotating speed is 220rpm, when saccharomycete content reaches 10
9During the CFU/ml left and right sides, stop fermentation, for subsequent use;
(2) choose above-mentioned each raw material, behind 50～60 ℃ of lower oven dry 2～3h, pulverize, cross the 80-120 mesh sieve, for subsequent use;
(3) take by weighing material powder after the pulverizing by above-mentioned prescription, add and account for the dregs of beans of Chinese medicine gross weight 4%, 10% corn flour, 10% wheat bran, add water to water content 50～55%, mix, at room temperature soak 24h, regulate about pH6.8, at 121 ℃ of lower steam sterilizing 20min, after the cooling, add respectively the candida utili bacterium bacterium liquid account for raw material gross weight 2.5%, 2.5% saccharomyces cerevisiae bacterium liquid and 2.5% S. cervisiae bacterium liquid, at 28～30 ℃ of fermentation 48h, stir once every 8h; When total viable count reaches 1.0 * 10
9Stop fermentation during CFU/ml, 50 ℃ of lower vacuum drying make livestock and poultry anti diar rhea feed addictive.
Embodiment 5
A kind of feed (chick is used) that contains livestock and poultry anti diar rhea feed addictive comprises the constituent of following percentage by weight: livestock and poultry anti diar rhea feed addictive 0.05% and auxiliary material 99.95%.Namely the addition of livestock and poultry anti diar rhea feed addictive is 500g in 1 ton of feed.Feed can specifically comprise the constituent of following parts by weight: by 0.05 part of the prepared fowl anti diar rhea feed addictive of the embodiment of the invention 1,68.00 parts of corns, 1.00 parts in inferior powder, 23.00 parts of dregs of beans, 2.00 parts of corn protein powders, 1.55 parts of greases, 1.40 parts of hydrogen calcium, 1.50 parts of stone flours, 0.40 part of salt, 0.03 part of phytase, 0.30 part of lysine, 0.30 part of methionine, 0.13 part of Choline Chloride, 0.04 part of B B-complex pre-mixing agent, 0.30 part of composite trace element pre-mixing agent.
Embodiment 6
A kind of feed (piglet is used) that contains livestock and poultry anti diar rhea feed addictive comprises the constituent of following percentage by weight: livestock and poultry anti diar rhea feed addictive 0.08% and auxiliary material 99.92%.Namely the addition of livestock and poultry anti diar rhea feed addictive is 800g in 1 ton of feed.Feed can specifically comprise the constituent of following parts by weight: by 0.08 part of the prepared livestock and poultry anti diar rhea feed addictive of the embodiment of the invention 2,62.92 parts of corns, 26.00 parts of Soybean Meaies, 8.00 parts of wheat brans, 0.90 part of stone flour, 1.00 parts of calcium monohydrogen phosphates, 0.20 part of FE-5,0.30 part of salt, 0.10 part in choline, 0.50 part of 0.5% piglet premix.
Embodiment 7
A kind of feed (chicken is used) that contains livestock and poultry anti diar rhea feed addictive comprises the constituent of following percentage by weight: livestock and poultry anti diar rhea feed addictive 0.10% and auxiliary material 99.9%.Namely the addition of livestock and poultry anti diar rhea feed addictive is 1000g in 1 ton of feed.Feed can specifically comprise the constituent of following parts by weight: by 0.10 part of the prepared livestock and poultry anti diar rhea feed addictive of the embodiment of the invention 4,58.20 parts of corns, 1.70 parts in inferior powder, 27.00 parts of dregs of beans, 7.00 parts of cotton dregs, 2.00 parts of corn protein powders, 1.00 parts of greases, 1.00 parts of hydrogen calcium, 1.20 parts of stone flours, 0.24 part of salt, 0.015 part of phytase, 0.14 part of lysine, 0.14 part of methionine, 0.10 part of Choline Chloride, 0.025 part of B B-complex pre-mixing agent, 0.14 part of composite trace element pre-mixing agent.
Livestock and poultry anti diar rhea feed addictive is as the application of veterinary drug, in the situation that livestock and poultry animal diarrhea, the use amount of above-mentioned livestock and poultry anti diar rhea feed addictive can be brought up to 0.1～1% adds in the feed, use as veterinary drug, the diarrhoea that the various viruses of livestock and poultry, bacterium, parasitic infection are caused all has significant result for the treatment of.
Below by concrete test example the beneficial effect of livestock and poultry anti diar rhea feed addictive of the present invention is described.
Test example 1
This test example is by embodiment 3 prepared livestock and poultry anti diar rhea feed addictives the effect assessment of weaned piglets and prevention diarrhoea to be tested.
1 materials and methods
1.1 the selection of experimental animal and grouping
Select the source identical, body weight close (17kg), 200 of Du who is in a good state of health * large * long three way cross market pig, be divided at random 5 processing (being designated as successively 1#, 2#, 3#, 4#, 5#), 4 repetitions are established in each processing, and each repeats 10 pigs, male and female half and half.
1.2 Test by Stages
Body weight 17～30kg stage is carried out this test.
1.3 experimental design and test daily ration
Nutritional need design corn-soybean meal basal diet according to NRC (1998) pig, in basal diet, add antibiotic and consist of 1 type daily ration, basal diet interpolation 1500ppm common Chinese preparation consists of 2 type daily rations, and (wherein the common Chinese preparation is: 5 parts in Radix Glycyrrhizae, 3 parts of quassias, 10 parts of the banksia rose, 6 parts of rhizoma zingiberis, 2 parts of Sophora alopecuroides, 3 parts of the Radixs Astragali, 3 parts of the barks of eucommia, 4 parts of dandelions, 2 parts of golden cypresses, 1 part in alum, 2 parts in Chinese ephedra, 1 part of medical stone, behind 50～60 ℃ of lower oven dry 2～3h, pulverize, cross the 80-120 mesh sieve and get final product), add respectively livestock and poultry anti diar rhea feed addictive 300ppm in the basal diet, 500ppm and 800ppm consist of 3,4,5 type daily rations are given respectively and are raised 1#, 2#, 3#, 4#, 5# processes the examination pig.Basal diet sees Table 2.
Table 1 experimental design
| Process | Additive |
| 1# | Basal diet+75ppm aureomycin+60ppm colistin sulfate |
| 2# | Basal diet+1500ppm common Chinese preparation |
| 3# | Basal diet+300ppm anti-diarrhea agents |
| 4# | Basal diet+500ppm anti-diarrhea agents |
| 5# | Basal diet+800ppm anti-diarrhea agents |
Table 2 test basal diet
1.4 daily management
The semi open model pig house, online flat foster, dry powder feed, free choice feeding and drinking-water; Conventional program immunity sterilization.The interior all mean temperatures of experimental period house are respectively 28.6 ℃, 29.3 ℃, 29.0 ℃, 27.2 ℃.
1.5 testing index and method
1.5.1 feed intake (kg/ head day): take repeating groups as unit, the inventory that accurate recording is each, the stage finishes same day, Bu Wu was after jin once fed intake, and adopted clear bag, clear channel process metering residual feed weight, determine each repeating groups food consumption, thereby calculate its feed intake.
1.5.2 daily gain (g/ head day): the second day morning of morning same day on-test, end, take repeating groups as unit, weigh on an empty stomach, by gained initial weight, last re-computation total augment weight, and then calculate daily gain.
1.5.3 feedstuff-meat ratio: by feed intake, daily gain, calculate and respectively repeat thin feedstuff-meat ratio.
1.5.4 death rate (%): take repeating groups as unit, it extremely washes in a pan the pig number accurate recording, then calculates each processed group death rate in experimental period.
2 results and analysis
Table 3 is on the * that affects of market pig (before the 30kg) production performance and diarrhea rate
* same column shoulder marking-up mother difference represents significant differences (P＜0.05), and same column shoulder marking-up parent phase is with representing difference not remarkable (P＞0.05).
By as seen from Table 3, compare with interpolation common Chinese processed group, the anti-diarrhea agents processed group of adding 800ppm can significantly improve piglet feed intake, daily gain, feed efficiency (P＜0.05), and each index of the anti-diarrhea agents processed group of interpolation 300ppm is suitable with the common Chinese preparation that adds 1500ppm; Feed intake, the daily gain of adding the anti-diarrhea agents processed group of 800ppm all increase than the antibiotic group, and diarrhea rate reduces, and feedstuff-meat ratio improves; Each index of anti-diarrhea agents processed group of adding 500ppm is suitable with the antibiotic group.Illustrate that livestock and poultry anti diar rhea feed addictive of the present invention can effectively improve the growth performance of piglet and reduce its diarrhea rate, the 500ppm anti-diarrhea agents is substitute antibiotics effectively.
Test example 2
This test example is the meat chick to be improved the effect assessment test of production performance and prevention diarrhoea by the livestock and poultry anti diar rhea feed addictive of embodiment 2 preparations.
Choose 1500 of the Luo Si 308 meat chicks of 1 age in days body condition health, be divided at random five processing (being designated as 1#, 2#, 3#, 4#, 5#), each processes 4 repetitions (being designated as A, B, C, D), and each repeats 75 chickens.49 days experimental periods, minute three phases, 0～21 age in days stage, 22～42 age in days stages, 43～49 age in days stages.Recommendation feeding standard according to this kind, nutritional need in conjunction with NRC (1994) meat chick, make three stage metabolizable energy concentration and be respectively 2.88Mcal/kg, 2.99Mcal/kg, 3.05Mcal/kg, crude protein content is 20.37%, 18.97%, 17.44% basal diet, (wherein the common Chinese preparation is: 5 parts of the banksia rose to add respectively common Chinese preparation 1000ppm in basal diet, 3 parts of rhizoma zingiberis, 8 parts in Radix Glycyrrhizae, 6 parts of quassias, 1 part of Sophora alopecuroide, 5 parts of the Radixs Astragali, 5 parts of the barks of eucommia, 3 parts of dandelions, 2 parts of kuh-sengs, 1 part in turmeric, 1 part of akebi, 1 part of the bighead atractylodes rhizome, 2 parts in alum, behind 50～60 ℃ of lower oven dry 2～3h, pulverize, cross the 80-120 mesh sieve and get final product), antibiotic Bacitracin Zinc 50ppm+ colistin sulfate 10ppm, livestock and poultry anti diar rhea feed addictive 300ppm, 500ppm, 800ppm, consist of 5 kinds of test daily rations and (be called successively 1#, 2#, 3#, 4#, the 5# daily ration), raised the chicken of above 5 processed group respectively, basal diet sees Table 4.Three-layer stereo is raised in cages, dry powder feed, and free choice feeding and drinking-water, routinely program immunity sterilization, normal condition is with weekly the chicken sterilization once, if meet strong wind weather, then wind is after at once sterilization.Adopt the hot-blast stove heating between brood time, all mean temperatures are respectively 32.7 ℃, 28.5 ℃, 27.6 ℃, 27.3 ℃, 25.6 ℃, 25.5 ℃, 25.8 ℃ in the house in 7 weeks.Second day after the off-test is weighed early morning on an empty stomach, detects the indexs such as body weight, feed intake, feedstuff-meat ratio, death rate, diarrhea rate.Experimental result sees Table 5.
Table 4 basal diet prescription
| The raising stage | The meat chicken | Chicken in the meat | The large chicken of meat |
| Corn 8% | 604.5 | 641 | 679 |
| Soybean Meal 43% | 280 | 210 | 180 |
| Cottonseed Meal 41% | 30 | 50 | 40 |
| Corn protein powder 60% | 30 | 40 | 40 |
[0098]
| Stone flour 37% | 13 | 12 | 11 |
| Calcium monohydrogen phosphate 21.5/16.5 | 18 | 14 | 13 |
| Soya-bean oil | 11 | 20 | 24 |
| Salt | 3.5 | 3 | 3 |
| 1% meat chicken premix | 10 | ||
| Chicken premix in 1% meat | 10 | ||
| The large chicken premix of l% meat | 10 | ||
| Amount to | 1000 | 1000 | 1000 |
| Fowl metabolizable energy (Mcal/kg) | 2.88 | 2.99 | 3.05 |
| Crude protein (%) | 20.37 | 18.97 | 17.44 |
| Calcium (%) | 1.01 | 0.86 | 0.79 |
| Available phosphorus (%) | 0.44 | 0.37 | 0.35 |
| Lysine (%) | 1.21 | 1.04 | 0.88 |
| Egg+cystine (%) | 0.87 | 0.76 | 0.66 |
Table 5 is on the * that affects of meat chick production performance and diarrhea rate
* between same column, shoulder marking-up parent phase represents difference not remarkable (P＞0.05) with the person, and alphabetical different persons represent difference not remarkable (P＜0.05).
Result of the test shows, add the 800ppm anti-diarrhea agents in the daily ration and can significantly improve meat chick daily gain (P＜0.05) than common Chinese preparation group and antibiotic group, the feedstuff-meat ratio of the anti-diarrhea agents group of 500ppm and 800ppm significantly is lower than common Chinese preparation group (P＜0.05), and is lower than antibiotic group (P＞0.05); The diarrhea rate of the anti-diarrhea agents group of 500ppm and 800ppm and death rate obviously are better than other each groups (P＜0.05).This illustrates that livestock and poultry anti diar rhea feed addictive of the present invention can improve the production performance of meat chick, and reduces its diarrhea rate, death rate, and effect is better than common Chinese preparation group, when addition 〉=500ppm, can replace antibiotic fully.
Test example 3
This test example is by embodiment 4 prepared livestock and poultry anti diar rhea feed addictives the effect assessment of weanling pig treatment diarrhoea to be tested.
1 materials and methods
1.1 experimental animal
120 of the weanling pigs of clinical definite trouble diarrhoea, body weight 8～15kg is divided into 2 groups at random, 60 every group.
1.2 test drug
Antibiotic: 10% Florfenicol+50% Doxycycline
Livestock and poultry anti-diarrhea agents: press the prepared livestock and poultry anti diar rhea feed addictive of embodiment 4
1.3 medication
The spice administration is admixed 10% Florfenicol 1000g/ ton+50% Doxycycline 600g/ ton and is made as the contrast daily ration in the basal diet, admix 3000g/ ton livestock and poultry anti-diarrhea agents and consist of the test daily ration in basal diet, feeds continuously 7 days.
1.4 observation index
Treatment situation: the pig's head number that stopped to suffer from diarrhoea in 3,5,7 days behind the recording medicine.
Cure rate: duration of test, the state of mind after the pig medication, appetite recover normal, without diarrhoea and dewatering symptom, and after 7 days without the recidivist, be judged to healing, calculate accordingly cure rate, cure rate=healing number/total number.
Efficient: the clinical symptoms of test pig are alleviated to some extent after medication, body weight than medication before enhancer be judged to effectively, efficient=treatment effective number/total number.
Inefficiency: what do not improve symptom of diarrhea in all 7 days is invalid, an inefficiency=number of failing to respond to any medical treatment/total number.
The death rate: dead pig number in the record duration of test 14 days, calculate the death rate, the death rate=death number/death rate.
2 result of the tests
Table 6 pair grice diarrhoea result for the treatment of
Clinical testing shows, curative effect is very fast in the antibiotic group 3 days, in 7 days treatment phases, livestock and poultry anti diar rhea feed addictive group is close with antibiotic group pig diarrhea treatment effect, after 7 days treatment phases, livestock and poultry anti diar rhea feed addictive group pig diarrhoea recurrence rate is lower than the antibiotic group, treats and observes in the full phase, livestock and poultry anti diar rhea feed addictive group pig cure rate is high, and recurrence rate is low all to be lower than the antibiotic group with the death rate.The diarrhea rate of livestock and poultry anti diar rhea feed addictive treatment weanling pig can reach more than 83%.
Claims (7)
1. livestock and poultry anti diar rhea feed addictive, it is characterized in that the method that adopts Chinese medicine to pass through probiotics fermention obtains, wherein Chinese medicine is by weight 1～10 part quassia, 1～5 part rhizoma zingiberis, 1～10 part Radix Glycyrrhizae, 1～10 part the banksia rose, and probio is any one or more than one combination in saccharomycete, lactic acid bacteria, the Bacillus.
2. livestock and poultry anti diar rhea feed addictive, it is characterized in that the method that adopts Chinese medicine to pass through probiotics fermention obtains, wherein Chinese medicine is 1～10 part quassia by weight, 1～5 part rhizoma zingiberis, 1～10 part Radix Glycyrrhizae, 1～10 part the banksia rose and to be selected from parts by weight be 1～10 part Sophora alopecuroide, 1～10 part the Radix Astragali, 1～10 part the bark of eucommia, 1～10 part dandelion, 1～5 part kuh-seng, 1～5 part golden cypress, 1～5 part turmeric, 1～5 part akebi, 1～5 part the bighead atractylodes rhizome, 1～5 part Chinese ephedra, 1～5 part alum, in the granatum of 1～5 part medical stone and 1～5 part one or more, probio is saccharomycete, lactic acid bacteria, in the Bacillus any one or more than one combinations.
3. livestock and poultry anti diar rhea feed addictive according to claim 1 and 2 is characterized in that described saccharomycete is candida utili, saccharomyces cerevisiae and/or brewer's yeast.
4. livestock and poultry anti diar rhea feed addictive according to claim 1 and 2 is characterized in that described lactic acid bacteria is VREF, lactobacillus lactis, lactobacillus acidophilus, Lactobacillus casei and/or lactobacillus bulgaricus.
5. livestock and poultry anti diar rhea feed addictive according to claim 1 and 2 is characterized in that described Bacillus is bacillus licheniformis, bacillus subtilis and/or bacillus cereus.
6. the preparation method of each described livestock and poultry anti diar rhea feed addictive according to claim 1～5 is characterized in that may further comprise the steps:
(1) Chinese medicine preliminary treatment
Choose Chinese medicine, behind 50～60 ℃ of lower oven dry 2～3h, pulverize, cross 80～120 mesh sieves, for subsequent use;
(2) preparation strain fermentating liquid
Each bacterial classification is inoculated in respectively in separately the optimum medium, cultivate under suitable condition primary seed solution, enlarge cultivation when each bacteria growing is transferred to respectively in the secondary seed fermentation tank during to exponential phase, stop when reaching exponential phase cultivating, for subsequent use;
(3) fermentation
Chinese medicine behind the crushing screening is mixed by proportioning, pack in the fermentation tank, add the dregs of beans, 0～15% corn flour, 5～20% wheat brans that account for Chinese medicine gross weight 0～5%, add water to water content between 50～70%, behind immersion 12～24h, regulate between the pH value 6.0～7.5, at 121 ℃ of lower steam sterilizing 15～20min, after the cooling, add the total strain liquid that accounts for traditional Chinese medicine quality gross weight 5%～20%, 28～37 ℃ fermented 36～96 hours, stirred once every 8h; After the fermentation ends, 35～50 ℃ of lower oven dry, namely get livestock and poultry anti diar rhea feed addictive; Wherein total strain liquid is that secondary seed solution by each bacterial classification is mixed and made into seed mixture liquid by equal proportion.
7. a feed that comprises each described livestock and poultry anti diar rhea feed addictive of claim 1～5 it is characterized in that being grouped into by the one-tenth of following part by weight: described livestock and poultry anti diar rhea feed addictive 0.02～0.2% and auxiliary material 99.8～99.98%.
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