CN110192595A - A kind of composite microorganism additive agent for milk cattle feed stuff and its preparation method and application - Google Patents
A kind of composite microorganism additive agent for milk cattle feed stuff and its preparation method and application Download PDFInfo
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- CN110192595A CN110192595A CN201910540318.1A CN201910540318A CN110192595A CN 110192595 A CN110192595 A CN 110192595A CN 201910540318 A CN201910540318 A CN 201910540318A CN 110192595 A CN110192595 A CN 110192595A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
Abstract
The invention discloses a kind of composite microorganism additive agent for milk cattle feed stuff and its preparation method and application, belong to technical field of animal.Composite microorganism additive agent for milk cattle feed stuff, the raw material including following parts by weight are prepared: 2~4 parts of 20~25 parts of bacillus subtilis microbial agent, 20~25 parts of lactic acid bacteria agent, 5~15 parts of yeast bacteria agent, 20~25 parts of protein supplement, 30~35 parts of enzyme auxiliary agent, 20~30 parts of algae replenishers and composite trace element intermixture, 35~40 parts of hawthorn powder and 30~35 parts of codonopsis pilosula powder.Complex microorganism additive provided by the invention is added in milk cow forage, can not only greatly improve the output of milk of milk cow, and also improves the quality of milk, therefore application of the complex microorganism additive in milk cow forage.
Description
Technical field
The present invention relates to technical field of animal, and in particular to a kind of composite microorganism additive agent for milk cattle feed stuff and its
Preparation method and application.
Background technique
Currently, additive for microbe feedstuff is the feed addictive of Ministry of Agriculture's approval, it is raw by improving microbial population of animal intestinal tract
State balances and plays beneficial effect, to improve a kind of product that animal health is horizontal, improves disease resistance, improves digestion power.
It is effectively to solve, disease is spread unchecked, germ drug resistance, immunocompetence decline, survival rate reduces, the effective means of culture efficiency decline.
It is the power of sustainable development of animal husbandry.
Probiotics have the advantages that its other medicine is irreplaceable, i.e. the effect of " illness is cured the disease, not sick diseases prevention, disease-free health care "
Fruit.It can be used healthy animal, to improve health conditions, and have dual regulation to diarrhea, constipation.
But microbe additive at present, ingredient is more single, and nutritional ingredient is relatively fixed.Therefore it provides a kind of compound micro-
Bio-additive enhances nutrition to milk cow comprehensively, improves milk crop and quality is asking for those skilled in the art's urgent need to resolve
Topic.
Summary of the invention
In view of this, being enriched the present invention provides a kind of composite microorganism additive agent for milk cattle feed stuff and preparation method thereof
The nutrition of milk cow forage improves the output of milk of milk cow.
To achieve the goals above, the present invention adopts the following technical scheme:
The present invention provides a kind of composite microorganism additive agent for milk cattle feed stuff, the raw material including following parts by weight is prepared into
It arrives: 20~25 parts of bacillus subtilis microbial agent, 20~25 parts of lactic acid bacteria agent, 5~15 parts of yeast bacteria agent, protein supplement 20
~25 parts, 30~35 parts of enzyme auxiliary agent, 20~30 parts of algae replenishers and 2~4 parts of composite trace element intermixture, hawthorn powder 35~
40 parts and 30~35 parts of codonopsis pilosula powder.
Preferably, the protein supplement includes the following components'mass percentage: fish protein powder 25%~45%, soybean
Powder 15%~35% and starch 25%~30%.
Preferably, the enzyme auxiliary agent includes the enzyme of following mass percent: maltose 15%~20%, cellulase
15%~25%, zytase 15%~20% and trypsase 5%~10%.
Preferably, the algae replenishers are one of kelp, microalgae and thallus laminariae or a variety of pulvis being prepared into.
Preferably, the composite trace element intermixture includes one of iron, copper, zinc, selenium, iodine, cobalt, manganese and sulphur or more
Kind.
Preferably, the raw material including following parts by weight is prepared: 23 parts of bacillus subtilis microbial agent, lactic acid bacteria agent 21
Part, 8 parts of yeast bacteria agent, 21 parts of protein supplement, 32 parts of enzyme auxiliary agent, 25 parts of algae replenishers and composite trace element intermixture
3 parts, 38 parts of hawthorn powder and 33 parts of codonopsis pilosula powder.
The present invention provides the preparation methods of the composite microorganism additive agent for milk cattle feed stuff, comprising the following steps:
(1) bacillus subtilis microbial agent, lactic acid bacteria agent, yeast bacteria agent, hawthorn powder, codonopsis pilosula powder and water are mixed, hair
Ferment culture obtains the first fermentation liquid；
(2) first fermentation liquid and algae replenishers are mixed, continues fermented and cultured, obtains the second fermentation liquid；
(3) it is carried out disinfection processing using pasteurization to second fermentation liquid, the fermentation liquid after being sterilized；
(4) fermentation liquid after enzyme auxiliary agent, protein supplement, composite trace element intermixture and the disinfection is mixed, is done
Dry concentration, obtains composite microorganism additive agent for milk cattle feed stuff.
Preferably, bacillus subtilis microbial agent, lactic acid bacteria agent, yeast bacteria agent, hawthorn powder, the gross mass of codonopsis pilosula powder and
The volume ratio of water is 1g: 5~10ml.
Preferably, it is 32~37 DEG C that step (1) fermented and cultured or step (2), which relay the temperature of continuous fermented and cultured, the hair
Ferment culture or continue fermented and cultured time be 10~14h, the fermented and cultured or continue fermented and cultured system pH be 5~
7。
The complex microorganism being prepared the present invention provides the complex microorganism additive or the preparation method adds
Add application of the agent in milk cow forage.
Composite microorganism additive agent for milk cattle feed stuff provided by the invention, including bacillus subtilis, lactic acid bacteria, yeast
Bacterium, protein supplement, enzyme auxiliary agent, algae replenishers and composite trace element intermixture, hawthorn powder and codonopsis pilosula powder.With the prior art
It compares, the composite microorganism additive agent for milk cattle feed stuff that the present invention discloses offer mixes bacteria agent by microorganism, coenzyme assists
It digests and assimilates and improves immunity of organisms；It is complete to milk cow by protein supplements, composite trace element intermixture, algae replenishers
Face supplements the nutrients；By traditional Chinese medicine ingredients such as Radix Codonopsis, hawthorn, milk cow digestive function is improved, promotes the product of milk production of cow and milk production
Matter.
The preparation method of composite microorganism additive agent for milk cattle feed stuff provided by the invention, preparation step is simple, repeatability
Good, manufacturing cycle is fast, and raw material is easy to get, and furthermore preparation method provided by the invention guarantees the safe detoxification of additive, improves
The quality of product.
Specific embodiment
The present invention provides a kind of composite microorganism additive agent for milk cattle feed stuff, the raw material including following parts by weight is prepared into
It arrives: 20~25 parts of bacillus subtilis microbial agent, 20~25 parts of lactic acid bacteria agent, 5~15 parts of yeast bacteria agent, protein supplement 20
~25 parts, 30~35 parts of enzyme auxiliary agent, 20~30 parts of algae replenishers and 2~4 parts of composite trace element intermixture, hawthorn powder 35~
40 parts and 30~35 parts of codonopsis pilosula powder.
The raw material of composite microorganism additive agent for milk cattle feed stuff provided by the invention includes bacillus subtilis microbial agent.By weight
Part meter is measured, the bacillus subtilis microbial agent is preferably 23 parts.The viable count of the bacillus subtilis microbial agent be 1~5 ×
1010CFU/g, more preferably 4 × 1010CFU/g.The present invention is not particularly limited the source of the bacillus subtilis microbial agent,
Using bacillus subtilis microbial agent known in the art.In embodiments of the present invention, the bacillus subtilis microbial agent
Purchased from Shandong Yi Tai Biotechnology Co., Ltd.The bacillus subtilis microbial agent acid and alkali-resistance, is able to maintain in acidic stomach environment
Activity, can tolerate saliva and bile attack, be in feed microbe can 100% through intestine and small intestine viable bacteria；Consumption intestines rapidly
Free oxygen in road causes enteron aisle hypoxemia, promotes to grow beneficial to anaerobic bacteria, and generate the organic acids such as lactic acid, reduces intestinal pH
Value inhibits other pathogenic bacteria to grow indirectly.The generation of disease is reduced, survival rate is improved.It is sent out in additive preparation process
When ferment culture, a large amount of multivitamins, organic acid, protease (especially alkali protease), carbohydrase, lipase, energy are generated
Complicated organic matter in degrading plant feed improves efficiency of feed utilization, prevents animal digestion not to promote to digest and assimilate
It is good, there are situations such as " feed are just " and occurs.
The raw material of composite microorganism additive agent for milk cattle feed stuff provided by the invention includes lactic acid bacteria agent.By weight
Meter, the lactic acid bacteria agent is preferably 21 parts.The viable count of the lactic acid bacteria agent is 0.5~9 × 1010CFU/g, more preferably
1~5 × 1010CFU/g, most preferably 2 × 1010CFU/g.The present invention is not particularly limited the source of the lactic acid bacteria agent,
Using lactic acid bacteria agent known in the art.In embodiments of the present invention, the lactic acid bacteria agent is micro- purchased from Guangzhou
First Biotechnology Co., Ltd.The lactic acid bacteria agent can improve milk cow intestinal environment, inhibit harmful bacteria breeding, adjust stomach
Intestinal flora balance；Improve animal immune ability, on the one hand can obvious activating macrophage phagocytosis, on the other hand due to
It can be equivalent to natural active immunity in intestinal colonisation.They can also stimulate peritoneal macrophages, induction to generate interferon, promote
Into cell division, antibody and promotion cellular immunity etc. are generated, so the non-specificity and specific immune response of body can be enhanced,
Improve the disease resistance of body；Also have and improve animal antibacterial action, has preferable inhibition to some spoilage organisms and psychrotropic bacteria
Effect controls vivotoxin, inhibits spoilage organisms growth in enteron aisle, can be used for preventing and treating diarrhea, diarrhea, enteritis, constipation and due to intestines
A variety of diseases caused by road dysfunction.
The raw material of composite microorganism additive agent for milk cattle feed stuff provided by the invention includes yeast bacteria agent.By weight
Meter, the yeast bacteria agent is preferably 8 parts.The viable count of the yeast bacteria agent is preferably 1~9 × 109CFU/g, more preferably
It is 1~5 × 109CFU/g, most preferably 2 × 109CFU/g.The present invention is not particularly limited the source of the yeast bacteria agent,
Using yeast bacteria agent known in the art.In embodiments of the present invention, the yeast bacteria agent is preced with purchased from Guangzhou agriculture
Biotechnology Co., Ltd.The yeast bacteria agent can effectively improve intestinal microflora structure, promote beneficial microorganism
Growth and breeding, the absorption for repelling pathogenic microorganism in intestinal mucosal surface are colonized；Promote growth, due to being rich in protein and B race
Vitamin, it is possible to provide protein, nucleic acid, vitamin and a variety of enzymes abundant have and provide nutritional ingredient, and it is agreeable to the taste to increase feed
Property, promote animal to the abilities of digestive and absorption of feed；Increase animal immunizing power, because being rich in manna oligosacchride, immunity of organism can be promoted
Function enhances premunition.
The raw material of composite microorganism additive agent for milk cattle feed stuff provided by the invention includes protein supplement.By weight
Meter, the protein supplement is preferably 21 parts.The protein supplement includes the following components'mass percentage: fish protein powder
25%~45%, soy meal 15%~35% and starch 25%~30%, more preferably fish protein powder 40%, 35% He of soy meal
Starch 25%.The protein supplement can increase the content of protein in feed, provide nutritional ingredient in feed.The albumen
The preparation method of replenishers mixes fish protein powder, soy meal and starch according to said ratio to obtain the final product.
The raw material of composite microorganism additive agent for milk cattle feed stuff provided by the invention includes enzyme auxiliary agent.By weight, institute
Stating enzyme preparation is 32 parts.The enzyme auxiliary agent preferably includes the enzyme of following mass percent: maltose 15%~30%, cellulose
Enzyme 15%~35%, zytase 15%-30% and trypsase 5%~20%, more preferably maltose 25%, cellulose
Enzyme 30%, zytase 30% and trypsase 15%.In the present invention, the effect of the enzyme auxiliary agent is to play enzymolysis,
The raw material decomposes of digestion will be not easy to decompose in raw material into small molecule, improve digestibility.The preparation method of the enzyme auxiliary agent
Maltose, cellulase, zytase and trypsase are mixed according to the above mentioned proportion and are prepared.
The raw material of composite microorganism additive agent for milk cattle feed stuff provided by the invention includes algae replenishers.By weight
Meter, the algae replenishers are preferably 25 parts.The algae replenishers are preferably one of kelp, microalgae and thallus laminariae or more
The pulvis that kind is prepared into.The compound amino acid abundant of the algae replenishers, fatty acid, polysaccharides, vitamin, minerals and micro-
The nutritional ingredient of the high values such as secondary element.
The raw material of composite microorganism additive agent for milk cattle feed stuff provided by the invention includes composite trace element intermixture.It presses
Parts by weight meter, the composite trace element intermixture are preferably 3 parts.The composite trace element intermixture preferably include iron, copper,
One of zinc, selenium, iodine, cobalt, manganese and sulphur are a variety of.
The raw material of composite microorganism additive agent for milk cattle feed stuff provided by the invention includes hawthorn powder.By weight, institute
Stating hawthorn powder is preferably 38 parts.The hawthorn powder, which is rich in, a large amount of tartaric acid, organic acid, hawthorn acid etc., but also not with addition
It measures excessively high, is easy to make digestive system pH value to decline, cause diarrhea, more even acid poisonings.
The raw material of composite microorganism additive agent for milk cattle feed stuff provided by the invention includes codonopsis pilosula powder.By weight, institute
Stating codonopsis pilosula powder is preferably 33 parts.The present invention is not particularly limited the source of the codonopsis pilosula powder, using party known in the art
Join powder.
The present invention provides the preparation methods of the composite microorganism additive agent for milk cattle feed stuff, comprising the following steps:
(1) bacillus subtilis microbial agent, lactic acid bacteria agent, yeast bacteria agent, hawthorn powder, codonopsis pilosula powder and water are mixed, hair
Ferment culture obtains the first fermentation liquid；
(2) first fermentation liquid and algae replenishers are mixed, continues fermented and cultured, obtains the second fermentation liquid；
(3) it is carried out disinfection processing using pasteurization to second fermentation liquid, the fermentation liquid after being sterilized；
(4) fermentation liquid after enzyme auxiliary agent, protein supplement, composite trace element intermixture and the disinfection is mixed, is done
Dry concentration, obtains composite microorganism additive agent for milk cattle feed stuff.
The present invention mixes bacillus subtilis microbial agent, lactic acid bacteria agent, yeast bacteria agent, hawthorn powder, codonopsis pilosula powder and water,
Fermented and cultured obtains the first fermentation liquid.
In the present invention, bacillus subtilis microbial agent, agent of lactic acid bacteria, yeast bacteria agent, hawthorn powder, the gross mass of codonopsis pilosula powder
Volume ratio with water is preferably 1g: 5~10ml, and more preferably 1g: 8ml.The temperature of fermented and cultured is preferably 32~37 DEG C, more excellent
It is selected as 35 DEG C.The time of the fermented and cultured is preferably 10~14h, more preferably 12h.The pH value of system when the fermented and cultured
Preferably 5~7, more preferably 6.On the one hand the fermented and cultured improves the viable count of above-mentioned three kinds of microbial inoculums and metabolite enriches
On the other hand degree is conducive to improve hawthorn powder, the stomach absorbability of codonopsis pilosula powder.
After obtaining the first fermentation liquid, the present invention mixes first fermentation liquid and algae replenishers, continues fermented and cultured,
Obtain the second fermentation liquid.
In the present invention, the time for continuing fermented and cultured is preferably 10~14h, more preferably 12h.It is described after supervention
The pH value of system is preferably 5~7 when ferment culture, and more preferably 6.The algae replenishers will by above-mentioned three kinds of microbial actions
Nutrient release, and the metabolite of microbial inoculum is enriched, a variety of nutriments abundant are provided for feed.
In the present invention, the temperature for continuing fermented and cultured is preferably 32~37 DEG C, and more preferably 35 DEG C.The fermentation
Culture or the time for continuing fermented and cultured are preferably 10~14h, more preferably 12h.The fermented and cultured continues fermented and cultured
System pH be preferably 5~7, more preferably 6.
The complex microorganism being prepared the present invention provides the complex microorganism additive or the preparation method adds
Add application of the agent in milk cow forage.
In the present invention, the addition quality of the complex microorganism additive accounts for the 1%~10% of milk cow basal feed, more
Preferably 5%.The preparation method of the milk cow forage is preferably by the complex microorganism additive of above-mentioned preparation and milk cow basis
Feed mixes to obtain the final product.The feeding method and scale of feeding of the milk cow forage are not particularly limited, using milk known in the art
The scale of feeding of ox feed.
Below with reference to embodiment to a kind of composite microorganism additive agent for milk cattle feed stuff provided by the invention and its preparation side
Method and application are described in detail, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Weigh following raw material: bacillus subtilis microbial agent 20kg, lactic acid bacteria agent 25kg, yeast bacteria agent 5kg, albumen are mended
Fill agent 25kg, enzyme auxiliary agent 30kg, algae replenishers 30kg and composite trace element intermixture 2kg, hawthorn powder 40kg and codonopsis pilosula powder
30kg.Wherein bacillus subtilis microbial agent viable count is 1 × 1010CFU/g；The viable count of lactic acid bacteria agent is 9 ×
1010CFU/g；The viable count of yeast bacteria agent is 5 × 109CFU/g.Protein supplement includes fish protein powder 35%, soy meal
35% and starch 30%.The enzyme auxiliary agent includes the enzyme of following mass percent: maltose 30%, cellulase 35%, wood
Dextranase 30% and trypsase 5%.The algae replenishers are kelp pulvis.
The preparation method of composite microorganism additive agent for milk cattle feed stuff the following steps are included:
(1) by bacillus subtilis microbial agent, lactic acid bacteria agent, yeast bacteria agent, hawthorn powder, codonopsis pilosula powder and water according to feed liquid
Than the ratio mixing for 1: 10, the fermented and cultured 14h at 32 DEG C obtains the first fermentation liquid；
(2) the first fermentation liquid and algae replenishers are mixed, continues fermented and cultured according to the method described above, obtains the second fermentation
Liquid；
(3) 25s is disinfected at 65 DEG C to second fermentation liquid using pasteurization, the fermentation after being sterilized
Liquid；
(4) fermentation liquid after enzyme auxiliary agent, protein supplement, composite trace element intermixture and the disinfection is mixed,
Dry concentration, obtains composite microorganism additive agent for milk cattle feed stuff at 40 DEG C.
Embodiment 2
Weigh following raw material: bacillus subtilis microbial agent 25kg, lactic acid bacteria agent 20kg, yeast bacteria agent 15kg, albumen
Replenishers 20kg, enzyme auxiliary agent 35kg, algae replenishers 20kg and composite trace element intermixture 4kg, hawthorn powder 35kg and Radix Codonopsis
Powder 35kg.Wherein bacillus subtilis microbial agent viable count is 5 × 1010CFU/g；The viable count of lactic acid bacteria agent is 7 ×
1010CFU/g；The viable count of yeast bacteria agent is 1 × 109CFU/g.Protein supplement includes fish protein powder 45%, soy meal
25% and starch~30%.The enzyme auxiliary agent preferably includes the enzyme of following mass percent: maltose 25%, cellulase
35%, zytase 30% and trypsase 10%.The algae replenishers are preferably microalgae pulvis.
The preparation method of composite microorganism additive agent for milk cattle feed stuff the following steps are included:
(1) by bacillus subtilis microbial agent, lactic acid bacteria agent, yeast bacteria agent, hawthorn powder, codonopsis pilosula powder and water according to feed liquid
Than the ratio mixing for 1: 5, the fermented and cultured 10h at 37 DEG C obtains the first fermentation liquid；
(2) the first fermentation liquid and algae replenishers are mixed, continues fermented and cultured according to the method described above, obtains the second fermentation
Liquid；
(3) 20s is disinfected at 70 DEG C to second fermentation liquid using pasteurization, the fermentation after being sterilized
Liquid；
(4) fermentation liquid after enzyme auxiliary agent, protein supplement, composite trace element intermixture and the disinfection is mixed,
Dry concentration, obtains composite microorganism additive agent for milk cattle feed stuff at 40 DEG C.
Embodiment 3
Weigh following raw material: bacillus subtilis microbial agent 23kg, lactic acid bacteria agent 21kg, yeast bacteria agent 8kg, albumen are mended
Fill agent 21kg, enzyme auxiliary agent 32kg, algae replenishers 25kg and composite trace element intermixture 3kg, hawthorn powder 38kg and codonopsis pilosula powder
33kg.Wherein bacillus subtilis microbial agent viable count is 4 × 1010CFU/g；The viable count of lactic acid bacteria agent is 2 × 1010CFU/
g；The viable count of yeast bacteria agent is 2 × 109CFU/g.Protein supplement includes fish protein powder 40%, soy meal 35% and starch
25%.The enzyme auxiliary agent includes the enzyme of following mass percent: maltose 25%, cellulase 30%, zytase 30%
With trypsase 15%.The algae replenishers are preferably thallus laminariae pulvis.
The preparation method of composite microorganism additive agent for milk cattle feed stuff the following steps are included:
(1) by bacillus subtilis microbial agent, lactic acid bacteria agent, yeast bacteria agent, hawthorn powder, codonopsis pilosula powder and water according to feed liquid
Than the ratio mixing for 1: 10, the fermented and cultured 12h at 35 DEG C obtains the first fermentation liquid；
(2) the first fermentation liquid and algae replenishers are mixed, continues fermented and cultured according to the method described above, obtains the second fermentation
Liquid；
(3) 25s is disinfected at 65 DEG C to second fermentation liquid using pasteurization, the fermentation after being sterilized
Liquid；
(4) fermentation liquid after enzyme auxiliary agent, protein supplement, composite trace element intermixture and the disinfection is mixed,
Dry concentration, obtains composite microorganism additive agent for milk cattle feed stuff at 40 DEG C.
Comparative example 1
Additive is prepared according to the method for embodiment 3, difference is to omit the addition of bacillus subtilis microbial agent.
Comparative example 2
Additive is prepared according to the method for embodiment 3, difference is to omit the addition of enzyme auxiliary agent.
Comparative example 3
Additive is prepared according to the method for embodiment 3, difference is to omit the addition of algae replenishers.
Embodiment 4
Composite microorganism additive agent for milk cattle feed stuff prepared by Examples 1 to 3 and comparative example 1~3 is according to mass ratio 5%
It is added in milk cow basal feed, 3 kinds of milk cow forages is made.
It is feeding object with 24 first 2 weeks away from production to 30 weeks after production milk cows of Heilungkiang dairy cow farm, at random
It is divided into 8 groups, every group 3, is numbered.Wherein the 1st group is feeding cow basal feed, and the 2nd~4 group feeds respectively containing implementation
The milk cow forage of complex microorganism additive prepared by example 1~3, the 5th~7 group of feeding prepares compound micro- containing comparative example 1~3
The milk cow forage of bio-additive, the 8th group of feeding Chinese medicine additive feed.Wherein Chinese medicine additive feed is application number
201711003893.5, it is entitled it is a kind of improve milk production of cow biological feed additive patent disclosed in composite Chinese herbal
The preparation method of feed addictive, composite Chinese herbal is: weigh Radix Codonopsis 15kg, Radix Astragali 15kg, seed of cowherb 15kg, stem pith of the rice-paper plant 6kg,
Honeysuckle 7kg, Fructus Forsythiae 6kg, dandelion 15kg, viola mandshurica 10kg, rhizoma atractylodis 6kg, Radix Glycyrrhizae 5kg.The above raw material is used into the sun
Exposure removes impurity, and being crushed to partial size is 80 mesh.Each medicinal material is mixed, is set spare at shady and cool drying.By Chinese medicine additive
Feed is added in basal feed according to 5% additive amount, obtains Chinese medicine additive feed.
The measuring method of the output of milk
The method for assessing the whole month output of milk with 3 days daily yieldings are monthly measured, records output of milk 3d in one month,
Middle minute interval 8~11 days calculates the whole month output of milk (kg) according to Formulas I
The whole month output of milk (kg)=(M1 × D1)+(M2 × D2)+(M3 × D3) Formulas I
Wherein, M1, M2, M3 are the measurement day whole day output of milk；D1, D2, D3 are to measure day interval in last time when time measurement day
Number of days.The output of milk the results are shown in Table 1.
To the milk testing lactose and dairy fat content collected every time, average lactose, the dairy fat content of each group milk cow, wherein cream
Sugar, butterfat assay respectively according to as defined in national food safety standard GB5413.5-2010 about " infant food and
About " in infant food and dairy products fatty as defined in the measuring method of lactose, sucrose in dairy products " and GB5413.3-2010
Measuring method " is detected.Lactose, dairy fat content the results are shown in Table 2.
The every cow head average daily milk production of 1 each group of table and the whole month output of milk
Average lactose content and average dairy fat content in 2 each group milk of table
By Tables 1 and 2 result it is found that complex microorganism additive provided by the invention is added in feed, make milk cow
Average daily milk production and the whole month output of milk are significantly increased with additivated blank group is not added, with control group and prior art report
The additive in road is compared and is also had a clear superiority.In terms of lactose and dairy fat content, complex microorganism additive provided by the invention
It is added in feed, substantially increases each group lactose and dairy fat content, has a clear superiority compared with other each groups.This
Show that complex microorganism additive provided by the invention not only has positive effect in terms of cow producing milk, but also also improves ox
The quality of milk.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of composite microorganism additive agent for milk cattle feed stuff, which is characterized in that the raw material including following parts by weight is prepared:
20~25 parts of bacillus subtilis microbial agent, 20~25 parts of lactic acid bacteria agent, 5~15 parts of yeast bacteria agent, protein supplement 20~
25 parts, 30~35 parts of enzyme auxiliary agent, 20~30 parts of algae replenishers and 2~4 parts of composite trace element intermixture, hawthorn powder 35~40
Part and 30~35 parts of codonopsis pilosula powder.
2. complex microorganism additive as described in claim 1, which is characterized in that the protein supplement includes following quality
The component of percentage: fish protein powder 25%~45%, soy meal 15%~35% and starch 25%~30%.
3. complex microorganism additive as described in claim 1, which is characterized in that the enzyme auxiliary agent includes following quality percentage
The enzyme of ratio: maltose 15%~20%, cellulase 15%~25%, zytase 15%~20% and trypsase 5%
~10%.
4. complex microorganism additive as described in claim 1, which is characterized in that the algae replenishers are kelp, microalgae
With one of thallus laminariae or a variety of pulvis being prepared into.
5. complex microorganism additive as described in claim 1, which is characterized in that the composite trace element intermixture includes
One of iron, copper, zinc, selenium, iodine, cobalt, manganese and sulphur are a variety of.
6. complex microorganism additive as claimed in any one of claims 1 to 5, wherein, which is characterized in that including following parts by weight
Raw material be prepared: 23 parts of bacillus subtilis microbial agent, 21 parts of lactic acid bacteria agent, 8 parts of yeast bacteria agent, protein supplement 21
Part, 32 parts of enzyme auxiliary agent, 25 parts of algae replenishers and 3 parts of composite trace element intermixture, 38 parts of hawthorn powder and 33 parts of codonopsis pilosula powder.
7. the preparation method of composite microorganism additive agent for milk cattle feed stuff described in claim 1~6 any one, feature exist
In, comprising the following steps:
(1) bacillus subtilis microbial agent, lactic acid bacteria agent, yeast bacteria agent, hawthorn powder, codonopsis pilosula powder and water are mixed, fermentation training
It supports, obtains the first fermentation liquid；
(2) first fermentation liquid and algae replenishers are mixed, continues fermented and cultured, obtains the second fermentation liquid；
(3) it is carried out disinfection processing using pasteurization to second fermentation liquid, the fermentation liquid after being sterilized；
(4) fermentation liquid after enzyme auxiliary agent, protein supplement, composite trace element intermixture and the disinfection is mixed, drying is dense
Contracting, obtains composite microorganism additive agent for milk cattle feed stuff.
8. preparation method as claimed in claim 7, which is characterized in that bacillus subtilis microbial agent, lactic acid bacteria agent, saccharomycete bacterium
Agent, hawthorn powder, the volume ratio of the gross mass of codonopsis pilosula powder and water are 1g: 5~10ml.
9. preparation method as claimed in claim 7, which is characterized in that step (1) fermented and cultured or step (2) relaying supervention ferment training
Feeding temperature be 32~37 DEG C, the fermented and cultured or continue fermented and cultured time be 10~14h, the fermented and cultured or after
The system pH of continuous fermented and cultured is 5~7.
10. being prepared described in complex microorganism additive described in claim 1~6 any one or claim 7~9 any one
Application of the complex microorganism additive that method is prepared in milk cow forage.
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Application publication date: 20190903 |