CN101190003A - High-efficiency biological active fodder additives products and producing method and application thereof - Google Patents
High-efficiency biological active fodder additives products and producing method and application thereof Download PDFInfo
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Abstract
The invention discloses a high efficiency bioactivity animal feed additive product and a production method and application thereof. The invention pertains to the field of feed additive, which more particularly relates to a high efficiency bioactivity animal feed additive product that comprises various biogenk and a production method and application thereof. The invention solves the technical problem of providing a high efficiency bioactivity animal feed additive product, and the invention solves another technical problem of providing the production method and application of the high efficiency bioactivity animal feed additive product. The ingredients of the product of the invention comprise active bacillus subtilis, active yeast, active plant lactobacillus bacteria, Beta-glucanase, xylanase, cellulose and amylase; the production method of the invention comprises cultivation preparation and compound inocula of microorganism inocula. The product of the invention can improve microecology balance of enteric canal of ruminants, enhance utilization ratio of the feed, improve milkability of milk cows and quality of the milk, improve body immunity of the milk cows, thus reducing enteric canal diseases and purifying raising environments.
Description
Technical field:
The invention belongs to feed additive field, particularly contain highly effective biological feed additive product and the production method and the application of multiple active bacteria formulation.
Background technology:
At present China's milk cow amount of livestock on hand is about more than 1,000 ten thousand, more than 900 ten thousand tons of milk yields, the milk cattle cultivating industry develop the development that also promotes and promoted China's feed and additive industry rapidly; But China's milk cow forage still rests in the simple mixing of three aniseed (being corn, wheat bran, grouts), causes single, the amino acid mismatch of protein feeds in the prescription, and the output of milk compares with American-European countries with quality that all there is a big difference.The research and development high-efficiency biological active fodder additives, for improving the level that China milk cow produces, improve the quality of milk, reduce the incidence of disease of milk cow gastrointestinal disease and mammitis, reduce the bad smell of fecaluria and the eliminating amount of fecaluria nitrogen, the effective utilization (promptly improve the utilization rate of cheap roughage, improve the utilization rate of nutriment in the feed etc.) that reduces environmental pollution and abundant raising feed resource has important application value.Popular in the market active bacteria formulation all is single bacterium basically, and be mainly used in the monogastric animal feed, the product that is used for ruminant (as milk cow) has only the single culture preparation of the 2-3 family of the U.S. and France to propagate and do breed popularization experiment in China at present, and the domestic enterprise that does not also produce similar products.Yeast, bacillus subtilis, Lactobacillus plantarum all are the probios that can be applied to feed additive industry, and various enzyme preparations that the probio metabolism produces and useful metabolism composition thereof are promoting digestion, stablize the gut flora environment, having effect preferably aspect the raising efficiency of feed utilization; Aspergillus awamori can metabolism produce the plurality of enzymes preparation that comprises dextranase, and its culture can effectively improve the effect of digesting and assimilating of feed as feed addictive.Development has scientific matching, and outstanding effect contain the strong composite feed additive of multiple beneficial microorganism and enzyme activity for promoting aquaculture, particularly milk cattle cultivating already has very important significance.
Summary of the invention:
The technical problem that the present invention solves provides a kind of high-efficiency biological active fodder additives products, and another technical problem that the present invention solves provides high-efficiency biological active fodder additives products production method and application thereof.
High living organism forage additive product consists of: bacillus subtilis 1.0～9.0 * 10 in every gram product
8Individual, saccharomycete 1.0～8.5 * 10
8Individual, Lactobacillus plantarum 1.3～8.5 * 10
8Individual, 1,4 beta-glucanase 200～250IU, zytase 50～150IU, cellulase 100～120IU, amylase 250～300IU.
The concrete production method step of product of the present invention is as follows:
1. the cultivation of microbial bacterial agent preparation: spreading cultivation also respectively, drying obtains yeast microbial inoculum, Lactobacillus plantarum agent and bacillus subtilis microbial agent, (1) bacillus subtilis microbial agent preparation: cultivate bacillus subtilis from the inclined-plane switching, seed liquor after spreading cultivation is step by step transferred in the fermentation tank, the control temperature is 28-30 ℃, ventilate and cultivated 19-24 hour, throughput is 2.0m
3/ minute; The fermentation centrifugation that finishes obtains wet thallus, adds the protective agent freeze drying and obtains microbial inoculum, and protective agent consists of 10% defatted milk, 5% lactose, 5% glycerine.
(2) saccharomycete fungicide preparation: slant strains obtains yeast starter liquid through the multistage technology that spreads cultivation of routine, and switching is gone in the fermentation tank, and the control temperature is 28 ℃, ventilates early stage to cultivating 14 hours, and throughput is controlled to be 2.0m
3/ minute, the later stage anaerobism was cultivated 15 hours; Zymotic fluid low temperature concentrates, and after carrier mixed, through the fluidized bed drying preparation, vehicle group became CaCO
340 parts, 20 parts in dextrin, 20 parts of corn protein powders.
(3) Lactobacillus plantarum agent preparation: the inclined-plane is cultivated the Lactobacillus plantarum seed liquor and is transferred in the fermentation tank, and the control temperature is 28-32 ℃, and anaerobism was cultivated 22 hours, and throughput is 2.0m
3/ minute; The fermentation centrifugation that finishes obtains wet thallus, adds the protective agent that consists of 10% defatted milk, 5% trehalose, 5% glycerine, obtains microbial inoculum by freeze drying, and moisture is lower than 10%.
(3) aspergillus awamori culture preparation: bacterial classification is cultivated, and solid fermentation is cultivated: spore liquid is inoculated in the aspergillus oryzae solid state fermentation compost, and 26-35 ℃ is cultured to mycelia and covers with compost, and the low temperature fluidized bed drying is pulverized dry thing;
2, microbial inoculum is composite: proportionally carry out above-mentioned various microbial inoculums and the agent of aspergillus awamori fermented and cultured composite, the microbial inoculum compound proportion is as follows: yeast microbial inoculum 15-25 part, Lactobacillus plantarum agent 25-40 part, bacillus subtilis microbial agent 10-25 part, aspergillus awamori culture 25-60 part.
The bacterial classification that the present invention adopts is as follows:
Bacillus subtilis (Bacillus subtilis subsp) CGMCC1.210
Saccharomyces cerevisiae (Saccharomyces cerevisiae) CGMCC2.610
Lactobacillus plantarum (Lactobacillus plantarum) CGMCC1.557
Aspergillus awamori (Aspergillus awamori) CGMCC3.350
The depositary institution of above-mentioned four kinds of bacterial classifications is Chinese common micro-organisms culture presevation administrative centers: address: No. 13 institutes of microbiology of the Chinese Academy of Sciences in Zhongguancun N 1st Lane, Beijing City; Postcode: 100080.
Enzyme activity unit IU is defined as the enzyme amount of catalysis 1 micromole's substrate conversion in 1 minute among the present invention.
Adopt microorganism plate count method to detect viable count among the present invention.
Adopting conventional enzyme activity detection method to detect enzyme among the present invention lives.
What microbial inoculum was composite among the present invention than the ratio of greater inequality example is: yeast microbial inoculum 15-20 part, Lactobacillus plantarum agent 30-40 part, bacillus subtilis microbial agent 15-25 part, aspergillus awamori culture 45-60 part.
The invention product adopts the microbial inoculum compound proportion technology of scientific research effectively to guarantee the reasonable composition and the ratio of various microbial bacterial agents in the product, simultaneously also guaranteed the composition and the effective content of enzyme preparation in the product, made that various enzymes can effectively promote digesting and assimilating of feed in milk cow is fed in the product; Compounded technology makes product significantly improve at the digestibility of milk cow forage, and the yield ratio comparison of unit consumption feed significantly improves according to having had, and output of milk cow and quality are improved and ensure.
The stable performance of invention product, safe in utilization, all there is not incompatibility with other feed addictive.Product of the present invention is stable and controllable for quality, non-environmental-pollution, and can improve immunity and can improve culture benefit.Product meets the requirement of green feed additive fully.The daily gain that is significantly improved and feed conversion rate effect.
Lactobacillus plantarum and saccharomycete have booster action to the digestion of animal gastrointestinal tract in the product of the present invention, saccharomycete growth and breeding for rumen microorganism in cud provides useful amino acid, B family vitamin and other trace element, increases the synthetic quantity of rumen microorganism protein.Lactobacillus plantarum discharges at the small intestine of milk cow, can consume the oxygen in the animal intestinal, pathogenic bacteria such as aerobic bacteria (mainly being Escherichia coli, salmonella etc.) harmful in the enteron aisle are suppressed, can also suppress the synthetic of ammonia and amine, benefit strengthens immunity, reach and improve milk cow intestines and stomach microbial ecological balance, help purpose healthy and the raising production performance; Dextranase, zytase, cellulase and the amylase of the effective dose that bacillus subtilis contains in enzyme that the enteron aisle metabolism produces and aspergillus awamori fermentation culture medium extremely helps digesting and assimilating of fiber-like feed in the ruminant feed such as milk cow, improve the microecological balance of intestine of ruminants, improve efficiency of feed utilization, improve milk production of cow, improve the quality of milk, improve the milk cow body immunity, reduce intestines problem, purify feeding environment.This feed addictive can be applicable to the milk cattle cultivating industry, can significantly improve milk crop and quality.
Product of the present invention can improve the milk cow body immunity, reduces the incidence of disease of recessive mastitis, intestines problem.At present, the recessive mastitis incidence of disease is than higher in China milk cows, in a single day milk cow suffers from mammitis just must the injection of antibiotics treatment, and the milk of having injected institute's output within the antibiotic milk cow 7 days all can not be sold (in the milk can residual antibiotic human body is had harm).Use product of the present invention can reduce the cow subclinical mastitis incidence of disease, feeding experiment shows that product of the present invention reduces the mammitis incidence of disease 70% (comparing with control group), obviously reduce the generation of mastitis for milk cows, reduce the antibiotic output of using and containing antibiotic milk, improved culturist's benefit indirectly.
Product of the present invention is to improving the level that China milk cow produces, and the effective utilization aspects such as (promptly improve the utilization rate of cheap roughage, improve the utilization rate of nutriment in the feed etc.) that fully improves feed resource all has very high value.
The present invention also is fit to feeding of other ruminants such as beef cattle, sheep.
The specific embodiment:
The following examples can make those skilled in the art more fully understand the present invention, but do not limit the present invention in any way.
Embodiment 1: high-efficiency activated feedstuff additive product
Product is composed as follows: the effective active components that contains in every gram product is: active bacillus subtilis 3.3 * 10
8Individual, active yeast 8.3 * 10
8Individual, Lactobacillus plantarum 1.3 * 10
8Individual, 1,4 beta-glucanase 200IU, zytase 50IU, cellulase 100IU, amylase 300IU.
Embodiment 2: example 1 described high-efficiency activated feedstuff additive product production method
Product processes mainly comprises the production and the complex process of microbial bacterial agent.
The production of microbial bacterial agent:
Saccharomycetic preparation method: to liquid shaking bottle, liquid shaking bottle is gone into fermentation tank through three grades of back switchings that spread cultivation and is enlarged cultivation from inclined-plane switching saccharomycete, cultivates the low temperature that finishes and concentrates, mixes with carrier, fluid bed drying process acquisition active dry yeasr microbial inoculum.
The cultivation of yeast cells: adopt the slant strains acquisition yeast cells that spreads cultivation step by step;
(1) first order seed is cultivated: the yeast slant strains is inserted in 500 ml shake flasks 100 milliliters of culture medium loading amounts, 120 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 18 hours;
(2) secondary seed is cultivated: first order seed is inserted 500 milliliters of secondary seeds according to 10% inoculum concentration shake in the bottle, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is inserted 5000 milliliters of three grades of seeds with 10% inoculum concentration shake in the bottle 1000 milliliters of culture medium loading amounts, 100 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 18 hours;
(4) first class seed pot is cultivated: it is the first class seed pot of 150L that three grades of seeds are inserted total measurement (volume) with 5% inoculum concentration, fermentation medium loading amount 100L, 30 ℃ of cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1: 0.5, tank pressure 0.05Mpa, incubation time 16 hours;
(5) fermentation tank culture: it is 3 tons of secondary seed jars that the first class seed pot bacterial classification is inserted total measurement (volume) with 5% inoculum concentration, 2 tons of fermentation medium loading amounts, 30 ℃ of condition of culture cultivation temperature, 100 rev/mins of mixing speeds, ventilation in early stage (V/V) 1: 0.5, tank pressure 0.05Mpa, early stage, incubation time was 12 hours; Later stage tank pressure 0.05Mpa, 50 rev/mins of mixing speeds, incubation time 8 hours, the yeast concentration of cultivating latter stage reaches 3.0 * 10
9Individual/m1.
(6) concentrate: zymotic fluid is concentrated in vacuo to 45% of original volume through low-temperature negative-pressure, obtains the saccharomycete concentrate.
(7) add carrier: in the saccharomycete concentrate, add the carrier that mixes, mix; The weight ratio of concentrate and carrier is 0.5-0.7: 1, and vehicle group becomes: CaCO
340 parts, 20 parts in dextrin, 20 parts of corn protein powders.
(8) drying: fluidized bed drying, 50 ℃ of baking temperatures.
Culture medium adopts 10% malt extract medium or adopts the molasses culture medium of adding inorganic salts such as ammonium sulfate, specifically cultivates production technology referring to Xiao Dongguang " production and the application technology of wine brewing active dry yeast ".
The preparation method of aspergillus awamori microbial inoculum:
Technical scheme is as follows:
Slant strains activation culture: the aspergillus awamori slant strains is transferred on the slant medium, cultivated 3 days for 27 ℃.
The solid first order seed is cultivated: picking aspergillus awamori slant strains inserts in 500 milliliters of triangular flasks that 100 gram culture mediums are housed carries out seed culture, cultivates for 30 ℃ to get final product in 3 days.
The cultivation of solid secondary seed: above-mentioned cultured consubstantiality first order seed stirring is carried out seed culture, condition of culture for adding behind the fragment in 5000 milliliters of triangular flasks that 1000 gram culture mediums are housed: 30 ℃ of cultivations got final product in 3 days.
Solid fermentation is cultivated: the secondary shake-flask seed is pulverized, added and mix the back in fermentation vat that sterilising medium is housed or the pallet and cultivate, bent material cultivation temperature is controlled at 26-35 ℃, humidity 80-90%, every 10 hours stirrings once, incubation time 6 days; Bent material culture technique commonly used is adopted in the cultivation of solid koji material; Treat that compost covers with mycelia and can finish to cultivate, culture medium is in advance through the thermophilic digestion sterilization treatment, and sterilising conditions is controlled 121 ℃ of temperature, 1 hour time.
Drying and crushing: the fermentation ends compost carries out drying on fluid bed or other drying equipments, baking temperature is controlled at 60 ℃, is dried to moisture below 10%, then solid culture medium is pulverized, and material is pulverized the aperture more than 60 orders.
Culture medium is formed: solid material: wheat bran 80%, and soya-bean cake powder 10%, cornstarch 10% adds the equivalent running water; Initial pH nature.
The preparation of bacillus subtilis microbial agent:
1. the acquisition of zymotic fluid: adopt slant strains to spread cultivation step by step and obtain fermentation of bacillus subtilis liquid;
(1) first order seed is cultivated: the bacillus subtilis slant strains is inserted in 500 ml shake flasks 100 milliliters of culture medium loading amounts, 180 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 24 hours;
(2) secondary seed is cultivated: first order seed is inserted 500 milliliters of secondary seeds according to 10% inoculum concentration shake in the bottle, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is inserted 5000 milliliters of three grades of seeds with 10% inoculum concentration shake in the bottle 1000 milliliters of culture medium loading amounts, 100 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 24 hours;
(4) first class seed pot is cultivated: it is the first class seed pot of 150L that three grades of seeds are inserted total measurement (volume) with 10% inoculum concentration, fermentation medium loading amount 100L, 28 ℃ of cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1: 0.5, tank pressure 0.05Mpa, incubation time 24 hours;
(5) fermented and cultured: it is 1.5 tons of secondary seed jars that the first class seed pot bacterial classification is inserted total measurement (volume) with 10% inoculum concentration, 1 ton of fermentation medium loading amount, 28 ℃ of condition of culture cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1: 0.5, tank pressure 0.05Mpa, incubation time 24 hours is cultivated and is finished bacteria concentration 5.0 * 10
8Individual/ml.
(6) centrifugation: adopt centrifuge to separate and obtain thalline.
(7) vacuum freeze drying: adopt drying process with atomizing to handle the acquisition dry bacteria after adding protective agent; Protective agent consists of defatted milk 10%, lactose 5%, glycerine 5%.Vacuum freeze-drying technique is write the culture presevation handbook referring to Institute of Microorganism, Academia Sinica, publishes in 1980. 610-653.
Culture medium is formed: glucose 6%, and yeast extract 1%, peptone 0.2%, CaCO3 1%, pH6.8.
The preparation method of Lactobacillus plantarum agent:
(1) first order seed is cultivated: the Lactobacillus plantarum bacterial classification is inserted in 500 ml shake flasks 100 milliliters of culture medium loading amounts, 30 ℃ of cultivation temperature, incubation time 24 hours;
(2) secondary seed is cultivated: first order seed is inserted 500 milliliters of secondary seeds according to 10% inoculum concentration shake in the bottle, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is inserted 5000 milliliters of three grades of seeds with 10% inoculum concentration shake in the bottle 1000 milliliters of culture medium loading amounts, 30 ℃ of cultivation temperature, incubation time 24 hours;
(4) first class seed pot is cultivated: it is the first class seed pot of 150L that three grades of seeds are inserted total measurement (volume) with 5% inoculum concentration, fermentation medium loading amount 100L, 30 ℃ of cultivation temperature, tank pressure 0.05Mpa, incubation time 18 hours;
(5) fermentation tank culture: it is 3 tons of secondary seed jars that the first class seed pot bacterial classification is inserted total measurement (volume) with 5% inoculum concentration, 2 tons of fermentation medium loading amounts, and 30 ℃ of condition of culture cultivation temperature, tank pressure 0.05Mpa, incubation time 24 hours is cultivated and is finished bacteria concentration 4.0 * 10
8Individual/ml.
(6) centrifugation: adopt centrifuge to separate and obtain thalline.
(7) vacuum freeze drying: add protective agent and adopt vacuum refrigeration to carry out dry treatment process, protective agent consists of the aqueous solution of defatted milk 10%, trehalose 5%, glycerine 5%.Vacuum freeze-drying technique is write culture presevation handbook, 1980.610-653. referring to Institute of Microorganism, Academia Sinica
Culture medium consists of: casein peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 0.5%, sodium acetate 0.5%, citric acid diamines 0.2%, Tween 800.1%, K2HPO40.2%, MgSO4.7H200.02%, MnSO4.H200.005%, CaCO32%, agar 1.5%, pH6.8.
Microbial inoculum is composite: above-mentioned various microbial inoculums are carried out according to following ratio composite, 25 parts of yeast microbial inoculums, 40 parts of Lactobacillus plantarum agent, 15 parts of bacillus subtilis microbial agents, 40 parts of aspergillus awamori cultures, composite microbial inoculum packing.The bacterial classification that adopts is as follows:
Bacillus subtilis (Bacillus subtilis subsp) CGMCC1.210
Saccharomyces cerevisiae (Saccharomyces cerevisiae) CGMCC2.610
Lactobacillus plantarum (Lactobacillus plantarum) CGMCC1.557
Aspergillus awamori (Aspergillus awamori) CGMCC3.350
Example 3 high-efficiency activated feedstuff additive products
Product is composed as follows: the effective active components that contains in every gram product is: active bacillus subtilis 7 * 10
8Individual, active yeast 1 * 10
8Individual, active plant lactobacillus 6 * 10
8Individual, 1,4 beta-glucanase 200IU, zytase 100IU, cellulase 120IU and amylase 250IU.
Example 4: example 3 described high-efficiency activated feedstuff additive product production methods
The basic production method is described with example 2, and wherein the microbial inoculum compound proportion is: above-mentioned various microbial inoculums are carried out according to following ratio composite, 23 parts of yeast microbial inoculums, 35 parts of Lactobacillus plantarum agent, 20 parts of bacillus subtilis microbial agents, 50 parts of aspergillus awamori cultures, composite microbial inoculum packing.
Example 5: high-efficiency activated feedstuff additive product production method
The basic production method is described with example 2, and wherein the microbial inoculum compound proportion is: above-mentioned various microbial inoculums are carried out according to following ratio composite, 20 parts of yeast microbial inoculums, 30 parts of Lactobacillus plantarum agent, 25 parts of bacillus subtilis microbial agents, 60 parts of aspergillus awamori cultures, composite microbial inoculum packing.
The experiment of example 6 product effects:
Selection and the experimental design of test ox: tested 7 days-August 19 April in 2006 and carry out in Tang Jia mountain range, Haidian District, Beijing City Guoqiang cattle farm, the random pair principle is taked in this test, with 40 oxen at random be divided into test group and control group, every group of 20 oxen, and adopt the method for counter-rotating trial design to eliminate the error that grouping causes, i.e. test was divided into for two phases, first phase test group is added the high-efficiency biological active fodder additives control group and is not added, after finishing 60 days experiment periods, control group is become test group, test group becomes control group, and for eliminating the influence of first phase group experiment, the phase of raising was designed to 10 days in advance before the second phase began.Finish and raised after date in 10 days in advance and enter second phase test, experimental period is 60 days.Every every day scale of feeding 50g.
Test method: per ten days experimental periods, measured once individual milk crop, and send milk cow center, Beijing to utilize Seris300combiFOSS milk composition analysis view to measure indexs such as butterfat percnetage in the milk and protein ratio in the morning, noon and afternoon sampling.
1: add the influence of high-efficiency biological active fodder additives to milk cow output
Result of the test sees Table 1
The variation * of table 1. test group and control group milk cow output represents P＜0.05, together down.
| Before the test | The first phase is when finishing | Than before the current period test ± | Testing comparison shines ± | Before the second phase test | Second phase off-test | Than before the current period test ± | Testing comparison shines ± | Full phase test group than control group ± | |
| Test group | 24.1 | 24.58 | 1.75 | 2.38* | 24.7 | 21.1 | -2.6 | 2.0* | 2.19* |
| Control group | 22.6 | 21.97 | -0.63 | 21.8 | 17.2 | -4.6 |
Because during on-test, the milk cow of test group and control group has all crossed the peak period, lactation rule according to milk cow, should be on a declining curve, from the result of the test of the first phase (60 days) as can be seen, owing to compare when having added high-efficiency biological active fodder additives with on-test, the output of milk of test group milk cow goes up not down, increase by 1.75 kilograms, and control group has descended 0.63 kilogram.Therefore, first phase test test group increases by 2.38 kilograms of milk yields (P＜0.05) than control group.Raised the phase in advance through 10 days after the counter-rotating intersection, enter second phase test (60 days), as can be seen from the test results, be that the test group or the milk cow of control group have all entered late lactation, the output of milk is all on a declining curve, the test group milk yield that adds high-efficiency biological active fodder additives descends 2.6 kilograms, the control group decline scope is bigger, reaches 4.6 kilograms, therefore, second phase test group is compared with control group, increases by 2.00 kilograms (P＜0.05).Full phase test group milk crop improves 2.19 kilograms than control group, and effect is obvious.
2. high-efficiency biological active fodder additives is to the influence of main milk cow composition
The results are shown in Table 2.
The test data of complete 4 months phases behind the interpolation high-efficiency biological active fodder additives, can significantly improve butterfat percnetage in the first phase test as can be seen from table 2, increases by 0.20 percentage point (P＜0.05).The Dan Congquan phase, butterfat percnetage had the trend of increase on average, simultaneously, and the trend that protein ratio also slightly increases, but difference is not remarkable.
3. use effect directly perceived
Use high-efficiency biological active fodder additives after 1 month, find that milk crop is more stable, (the group of Weiing does not decline by a big margin, because the test ox has spent peak of lactation), butterfat percnetage has also increased, and effect is fine.From the milk cow appearance, the hair of the milk cow of hello high-efficiency biological active fodder additives is bright, and appetite is good, and the mammitis incidence of disease reduces.
Table 2 high-efficiency biological active fodder additives is to the influence of test group and control group butterfat percnetage, protein ratio
3. use effect directly perceived
Use high-efficiency biological active fodder additives after 1 month, find that milk crop is more stable, (the group of Weiing does not decline by a big margin, because the test ox has spent peak of lactation), butterfat percnetage has also increased, and effect is fine.From the milk cow appearance, the hair of the milk cow of hello high-efficiency biological active fodder additives is bright, and appetite is good, and the mammitis incidence of disease reduces.
Example 7 effect tests
25 bullock tests are added product 40g by every every day in daily ration, fed 60 days, and the result shows experimental group group daily gain 972.7g, control group daily gain 746.8g, and difference is (P＜0.01) extremely significantly.After feeding 5 days, its ight soil dry with feed before compare, reduce 30%.
Claims (8)
1. a high-efficiency biological active fodder additives products is characterized in that bacillus subtilis is 1.0～9.0 * 10 in every gram product
8Individual, saccharomycete 1.0～8.5 * 10
8Individual, Lactobacillus plantarum 1.3～8.5 * 10
8Individual, 1,4 beta-glucanase 200～250IU, zytase 50～150IU, cellulase 100～120IU, amylase 250～300IU; Its production method comprises that the preparation of microbial bacterial agent and microbial inoculum are composite.
2. high-efficiency biological active fodder additives products according to claim 1 is characterized in that the composite ratio of microbial inoculum is: yeast microbial inoculum 15-25 part, Lactobacillus plantarum agent 25-40 part, bacillus subtilis microbial agent 10-25 part, aspergillus awamori culture 25-60 part.
3. high-efficiency biological active fodder additives products according to claim 1 is characterized in that every gram product contains bacillus subtilis 3.3 * 10
8Individual, saccharomycete 8.3 * 10
8Individual, Lactobacillus plantarum 1.3 * 10
8Individual, 1,4 beta-glucanase 200IU, zytase 50IU, cellulase 100IU, amylase 300IU.
4. high-efficiency biological active fodder additives products production method as claimed in claim 1 comprises the steps: the cultivation preparation of (1) microbial bacterial agent: dry yeast microbial inoculum, agent of lactic acid bacteria and the bacillus subtilis microbial agent of obtaining that spread cultivation respectively,
A hay bacillus fungicide preparation: cultivate hay bacillus from the inclined-plane switching, the fermentation tank that spreads cultivation step by step, the centrifugation interpolation protective agent freeze drying acquisition microbial inoculum that finishes ferments;
B saccharomycete fungicide preparation: slant strains concentrates through the multistage acquisition yeast fermentation broth that spreads cultivation, mixed carrier, and fluidized bed drying prepares microbial inoculum;
C Lactobacillus plantarum agent preparation: the inclined-plane is cultivated lactic acid bacteria step by step and is obtained zymotic fluid, and centrifugation obtains wet thallus, adds the protective agent that consists of 10% defatted milk, 5% trehalose, 5% glycerine, and freeze drying obtains microbial inoculum;
The preparation of d aspergillus awamori culture: bacterial classification is cultivated, and solid fermentation is cultivated, and dry thing is pulverized in low temperature drying;
(2) microbial inoculum is composite: above-mentioned various microbial inoculums are composite, and ratio is yeast microbial inoculum 15-25 part, Lactobacillus plantarum agent 25-40 part, bacillus subtilis microbial agent 10-25 part, aspergillus awamori culture 25-60 part.
5. high-efficiency biological active fodder additives products production method according to claim 3, it is characterized in that the microbial inoculum compound proportion is yeast microbial inoculum 15-20 part, Lactobacillus plantarum agent 30-40 part, bacillus subtilis microbial agent 15-25 part, aspergillus awamori culture 45-60 part.
6. according to claim 3 or 4 described high-efficiency biological active fodder additives products production methods, it is characterized in that the microbial inoculum compound proportion is 25 parts of yeast microbial inoculums, 40 parts of Lactobacillus plantarum agent, 15 parts of bacillus subtilis microbial agents, 40 parts of aspergillus awamori cultures.
7. according to claim 3 or 4 described high-efficiency biological active fodder additives products production methods, it is characterized in that the microbial inoculum compound proportion is 23 parts of yeast microbial inoculums, 35 parts of Lactobacillus plantarum agent, 20 parts of bacillus subtilis microbial agents, 50 parts of aspergillus awamori cultures.
8. the application of high-efficiency biological active fodder additives products as claimed in claim 1 in raising dairy cattle.
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