CN101244184A - Enteron modifying agent, preparation method and application thereof - Google Patents
Enteron modifying agent, preparation method and application thereof Download PDFInfo
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- CN101244184A CN101244184A CNA2007100905456A CN200710090545A CN101244184A CN 101244184 A CN101244184 A CN 101244184A CN A2007100905456 A CNA2007100905456 A CN A2007100905456A CN 200710090545 A CN200710090545 A CN 200710090545A CN 101244184 A CN101244184 A CN 101244184A
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Abstract
The invention relate to a digestive tract regulator, comprising 13 to 50 portions of culture medium containing various kinds of strain, and 1 to 9 portions of extraction from Chinese herbal medicines, and 45 to 85 portions of accessories, wherein the Chinese herbal medicines are composed of astragalus, aloe, medlar, heartleaf houtluynia, purslane or one kind of uncaria; the strain is germ bacillus named BB bacterial strain with preservation number of CGMCC No.1007. The digestive tract regulator has an advantage of being able to be used as enzyme preparation for livestock and poultry feeding and as additives for various livestock and poultry forages.
Description
Technical field
The present invention relates to a kind of enteron modifying agent and its production and use.
Background technology
Since finding that feeding antibiotic can be by influencing the enteric microorganism initial stage in the intravital field planting of host, thereby play after the somatotrophic effect, the antibiotic animal husbandry aspect that is applied in has produced huge economic benefit.But because antibiotic a large amount of uses, the negative effect of Chu Xianing also becomes increasingly conspicuous thereupon, and it is residual in animal product to show as pathogenic microorganism generation drug resistance and medicine, thereby human beings'health is produced potential harm.Therefore, the research and development of antibiotic succedaneum probiotic bacteria, enzyme preparation, acid supplement etc. become the focus of green feed additive in recent years, and wherein probiotic bacteria receives much concern.
Application number 00133695.9 discloses additive for microbe feedstuff and has carried lactobacillus viridescens, the pure bacterium of lactobacillus coprophilus, yeast, bacillus subtilis through amplification culture of culture medium, secondary amplification culture, with Testa oryzae, Testa Tritici absorption bacterium liquid by screening, heap fermentation, oven dry and reach the biology feed additive of strain quantity 8～1,000,000,000/gram.Can improve the function of livestock and poultry animal intestinal digestive system, make flora equilibrium in the intestinal, reduce the antibiotics use amount, improve the quality of animal products, improve absorption and the conversion ratio of poultry feedstuff; Save feedstuff, reduce feed cost, marketing period shifts to an earlier date, and laying rate improves, and is natural environment-friendly and green environment-friendly products.
Application number 00114573.8 discloses the preparation of the additive for farm animal feed that contains microorganism by filamentous fungi 30%, yeast 10%, and bacillus cereus 50%, lactobacillus 5%, photosynthetic bacteria 5% is formed.Preceding four kinds of bacterium respectively under 28～32 ℃ of temperature environments, solid or liquid fermentation 40～50 hours; Wherein lactobacillus was by 1: 1 adding inserts; In 35～50 ℃ of environment, dry, pulverize, sieve through 40～60 orders; Photosynthetic bacteria carries out seeing under 20 ℃ of environment of light; Adding minerals and vitamins, mix homogeneously are made.It can promote growth of animals or poultry; Improve efficiency of feed utilization, fowl poultry immune function, breeding and survival rate; Detoxification is good, can better get rid of the pollution of feces of livestock and poultry to environment.
Delivered on 2006 the 33rd the 9th phases of volume " Chinese animal and veterinary " and be entitled as " antibiotic property of Chinese herbal medicine additive and growth promotion Research on effect " literary composition, mentioned in the literary composition and used Radix Codonopsis, the Radix Astragali, Fructus Lycii, Folium Eucommiae, Massa Medicata Fermentata, 16 flavor Chinese medicine development pig's feed additives such as Fructus Crataegi, the result shows, conventional powder of this Chinese herbal medicine additive and extract formulation all can promote the probiotics in the intestine of young pigs: as the propagation of bacillus bifidus and lactobacillus, simultaneously for the harmful bacterium in the intestinal: as staphylococcus, enterobacteria has the obvious suppression effect, therefore can promote growth in piglets to grow.Bacillus cereus is a kind of as probiotic bacteria, and the effect of preparation in animal feed production that contains it is the focus that the research and production personnel of animal husbandry are paid close attention to.
Summary of the invention
The objective of the invention is to, a kind of enteron modifying agent is provided, have direct enhancing non-specific immunity function and come prevent disease thereby this enteron modifying agent can be used for regulating in the animal intestine microecological balance, promote the growth of animal effect and improve food conversion ratio and play indirectly.
Another order of the present invention is, a kind of preparation method of described enteron modifying agent is provided.
An order more of the present invention is, a kind of purposes of described enteron modifying agent is provided.
One aspect of the present invention has provided a kind of enteron modifying agent, and wherein above-mentioned regulator comprises and contains culture of strains liquid: 13～50 parts;
1～9 part of Chinese herbal medicine extract, above-mentioned Chinese herbal medicine are a kind of in the Radix Astragali, Aloe, Fructus Lycii, Herba Houttuyniae, Herba Portulacae or the Ramulus Uncariae Cum Uncis;
Adjuvant: 45～85 parts;
Above-mentioned strain is bacillus cereus (Bacillus), is called the BB bacterial strain, and preserving number is CGMCC No.1007.
This bacterial strain is called for short at China Committee for Culture Collection of Microorganisms common micro-organisms center in JIUYUE in 2003 22 days: CGMCC carries out preservation, and preserving number is CGMCC No.1007.This thalline becomes shaft-like, seldom chaining; Even dyeing.The flagellum adnation.0.7 micron * 1.4～1.7 microns of endospores; A little less than the surface colour of free spore.Spore coat equator row during sprouting.
Bacterium colony begins circularly when cultivating on culture medium, and smooth surface is opaque; Bacterium colony frills behind the later stage formation spore, becomes cream color.
The enteron modifying agent that another embodiment of the invention provides, adjuvant wherein are precipitated calcium carbonate, bean cake, Testa Tritici, fish flour.
The enteron modifying agent that another embodiment of the present invention provides, wherein above-mentioned Chinese herbal medicine are a kind of in Aloe, Herba Houttuyniae, Herba Portulacae or the Ramulus Uncariae Cum Uncis.
The enteron modifying agent that another embodiment of the present invention provides, wherein above-mentioned culture fluid are the glucose nutrient broth.
Another embodiment of the present invention has provided enteron modifying agent, and wherein above-mentioned Chinese herbal medicine extract is to obtain by the following method:
A. a kind of in the described Chinese herbal medicine sheared, cleans, obtain pending natural Chinese medicinal herb plant;
B. pending natural Chinese medicinal herb is distilled with water vapour, and the Chinese herbal medicine residue that obtains after the distillation is merged;
C. the Chinese herbal medicine residue water that will obtain after will merging decocts and extracts, and obtains extracting solution;
D. the precipitate that obtains wetting with the described extracting solution of 85% ethanol precipitation;
E. described wet precipitate is carried out spray drying and obtain described Chinese herbal medicine extract.
Enteron modifying agent provided by the invention, enteron modifying agent wherein is used to regulate the colony balance of animal alimentary canal, control intestinal balance.Enteron modifying agent can effectively be regulated and control the animal alimentary canal flora, the control intestinal tract disease, reduce diarrhea rate and fowl and animal excrement moisture, obviously improve the colony house environment, enteron modifying agent of the present invention can improve the growth performance of animal simultaneously, improve the regulator remuneration, easy to use.
Because the Chinese medicine extract composition in the enteron modifying agent of the present invention contains abundant mineral element and vitamin, help the electrolyte balance of daily ration, because the probiotics among the present invention can significantly reduce the coliform count in the intestinal, and can prevent the transfer of pathogen location, the reparation in facilitating digestion road, the microecological balance and the normal physiological function that enteric microorganism are formed again more help animal body, so enteron modifying agent of the present invention can effectively reduce coliform count, make enteric microorganism form the microecological balance that more helps animal body again and keep the intestinal microecology balance.
Another aspect of the present invention has provided a kind of method of enteron modifying agent, and wherein, described regulator prepares by following method:
A. the BB inoculation of described bacillus cereus is cultivated in culture medium, as inoculum;
B. then described inoculum is inoculated in another kind of culture medium, and adds in the described Chinese herbal medicine extract and cultivate, make culture fluid;
C. provide oxygen to described culture fluid, the culture fluid of described interpolation oxygen is incubated, and stirs simultaneously;
D. in the culture fluid that finishes insulation, add adjuvant, mix;
E. mixed culture fluid is dried, and pulverizes promptly to get described enteron modifying agent.
The invention provides another kind of method, the culture fluid that wherein adds oxygen is incubated 20～40 hours under 30～40 ℃ of temperature, and the speed that stirs is 60～100 rev/mins.
The invention provides another method, the condition of culture of BB bacterial strain in culture medium of bacillus cereus wherein is in 37 ℃ of calorstats, cultivates 3 days.
The invention provides another method, wherein another kind of culture medium is the glucose nutrient broth, and it is to be grouped into by following one-tenth: peptone 10g, Carnis Bovis seu Bubali cream 5g, sodium chloride 5g, glucose 2.5g, agar 15g, distilled water 1000ml.
The enteron modifying agent that another aspect of the present invention provides, enteron modifying agent wherein is used to regulate the colony balance of animal alimentary canal, control intestinal balance.
Preserving number in the enteron modifying agent among the present invention is the bacillus cereus (Bacillus) of CGMCC No.1007, and the BB bacterial strain has following physiology characteristic:
| Antibiotic | Minimal inhibitory concentration (μ g/ml) | Minimal bactericidal concentration (μ g/ml) |
| Kitasamycin | 3000 | 3000 |
| Quinocetone | 250 | 1000 |
| Chlortetracycline | ＞1000 | ＞1000 |
| Bacitracin zinc | 500 | ＞1000 |
| Olaquindox | 500 | 500 |
| Colistine sulfate | 250 | ＞1000 |
| Bambermycin | ＞625 | ＞625 |
| Arsanilic acid | ＞1000 | ＞1000 |
| Salinomycin | 715 | 715 |
| Virginiamycin | 65 | 200 |
| Herba Origani oil | 5000 | 11000 |
Hot resistance test result is as follows:
| Temperature (℃) | 70 | 80 | 90 | 95 |
| Survival rate (%) | 100％ | 98 | 92 | 89 |
The anti-pH of bacterial strain, cholate result of the test are as follows:
The anti-metal ion test of bacterial strain (mg/Kg)
Enteron modifying agent of the present invention, wherein preserving number is the bacillus cereus (Bacillus) of CGMCC No.1007, the BB bacterial strain, can produce lactic acid, volatile fatty acid and protein and peptide are picked anti-material, thereby suppress harmful microbe growth and breeding such as escherichia coli and invasive organism is produced the anti-effect of picking, this bacillus cereus has stronger protease, lipase and amylase activity, some pectin of degrading, carboxymethyl cellulose, complicated vegetality carbohydrate such as Poly Gal A Galacturonan, in addition, this bacillus cereus can promote gut-associated lymphoid tissue, help to keep the reaction " SBR " that these lymphoid tissues are in height, the growth of immune organ is speeded.Improve antibody horizontal, improve the ratio of splenic T, bone-marrow-derived lymphocyte, the quantity of T, bone-marrow-derived lymphocyte increases, and reduces SAC, has improved the body fluid and the cellular immune level of animal body;
Chinese medicine extraction composition among the present invention promotes the material of digesting and assimilating of enteral nutrition material, promote glucose content and total content of triglyceride rising in the piglet serum, promptly promote piglet to increase digestibility and utilization to nutrient substance in the daily ration, can reduce the nutrient matrix of intestinal back segment for the bacterial growth breeding, these Chinese medicine extraction compositions also can improve the piglet RBC number simultaneously, The Total albumen content, serum globulin content and the level that improves complement 3 and complement 4, improve humoral immunity and the cellular immunity of ablactational baby pig simultaneously, serum cholesterol content is risen.
Enteron modifying agent of the present invention can be used as the enzyme preparation of raising livestock and poultry animal, can be widely used in the feed additive of various livestock and poultry animals.
Enteron modifying agent of the present invention can improve feed digestibility and conversion ratio greatly, and can put forward the output of letting animals feed, can save feedstuff simultaneously, reduces feed cost.
The specific embodiment
Following embodiment further describes the present invention, but described embodiment only is used to illustrate the present invention rather than restriction the present invention.
Embodiment 1
The used strain of stating of present embodiment belongs to bacillus cereus (Bacillus), is called the BB bacterial strain, and in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) preservation, preserving number was CGMCC No.1007 to this bacterial strain in JIUYUE in 2003 22 days.
(BB) can obtain by biofermentation technique by described bacillus cereus.At first described Bacillus strain (BB) is cultivated, method is as follows:
Earlier with the BB inoculation of bacillus cereus in culture medium, in 37 ℃ of calorstats, cultivated 3 days, as inoculum.Described slant medium is: peptone 10g, Carnis Bovis seu Bubali cream 5g, sodium chloride 5g, glucose 2.5g, agar 15g, distilled water 1000ml.
Then inoculum is inoculated in the glucose nutrient broth, the composition of described glucose nutrient broth is: peptone 10g, Carnis Bovis seu Bubali cream 5g, sodium chloride 5g, glucose 2.5g, distilled water 1000ml, in the glucose nutrient broth, add Aloe extract 79g, make culture fluid.
Above-mentioned Aloe extract obtains by the following method: Aloe is sheared, cleans, then distill with steam, the Aloe residue that obtains after the distillation merges, Aloe residue water after will merging again decocts and extracts, obtain Aloe extraction solution, the precipitate that ethanol precipitation Aloe extraction solution with 85% obtains wetting carries out spray drying to above-mentioned wet precipitate, obtains being used for adding to the Aloe extract of glucose nutrient broth.
Oxygen is provided in culture fluid, and the culture fluid that adds oxygen is incubated 40 hours in 30 ℃, stirs in insulation, and the speed of stirring is 60 rev/mins, makes culture fluid; The bean cake that adds 0.9 times of glucose nutritional solution weight in the culture fluid that finishes insulation mixes as adjuvant, dries mixed culture fluid, and with its pulverizing, obtains enteron modifying agent.The medicament that resulting enteron modifying agent is used for colony balance in reconciling animal intestinal uses.
Embodiment 2
The preparation method of enteron modifying agent is with embodiment 1, different is, in the glucose nutrient broth, add Herba Houttuyniae extract 140g, behind the culture fluid of making, in culture fluid, add oxygen, the culture fluid that adds oxygen was 35 ℃ of insulations 30 hours, stir simultaneously, not 80 rev/mins of the speed that stirs, adjuvant in the enteron modifying agent is a precipitated calcium carbonate, the quality of precipitated calcium carbonate and glucose nutrient broth is for to mix in 3: 2 ratio, and the therapeutic agent that resulting enteron modifying agent is used for colony balance in reconciling animal intestinal uses.
Embodiment 3
The preparation method of enteron modifying agent is with embodiment 1, different is, different is, in the glucose nutrient broth, add Herba portulacae extract 184g, behind the culture fluid of making, in culture fluid, add oxygen, the culture fluid that adds oxygen was 40 ℃ of insulations 20 hours, stir simultaneously, the speed that stirs is 100 rev/mins, adjuvant in the enteron modifying agent is a Testa Tritici, and the quality of Testa Tritici and glucose nutrient broth is mixed by 6: 1 mass ratio, and the medicament that resulting regulator is used for colony balance in reconciling animal intestinal uses.
Embodiment 4
The preparation method of enteron modifying agent is with embodiment 1, different is, different is, in the glucose nutrient broth, add Ramulus Uncariae Cum Uncis extract 102g, behind the culture fluid of making, in culture fluid, add oxygen, the culture fluid that adds oxygen was 40 ℃ of insulations 20 hours, stir simultaneously, not 100 rev/mins of the speed that stirs, adjuvant in the enteron modifying agent is a Testa Tritici, and the quality of Testa Tritici and glucose nutrient broth is mixed by 1: 1 mass ratio, and the medicament that resulting enteron modifying agent is used for colony balance in reconciling animal intestinal uses.
Experimental example 1
This experimental example relates to the influence of enteron modifying agent of the present invention to piglet fertility performance and feedstuff apparent digestibility.
1 materials and methods
1.1 enzyme preparation
The present embodiment used enzyme preparation is an enteron modifying agent of the present invention.
1.2 experimental animal
Plan is selected 72 of piglets for use, body weight 15.76 ± 0.34kg, and male and female half and half is divided into 3 groups by the completely random block design, every group of 6 repetitions, each repeats 4, and the male and female subfield is fed.
1.3 test daily ration
Test is divided into 3 processing, i.e. matched group (basal diet of feeding), animal feed group 1 (basal diet of feeding+0.01% enzyme preparation) and animal feed group 2 (basal diet of feeding+0.05% enzyme preparation).The raw material of basal diet is formed and nutritional labeling sees Table 1.Cr
2O
3For measuring the external source indicator of feed digestibility.
The constituent of table 1 test daily ration
1Premix material is formed every kilogram of daily ration and is contained:: vitamin A, 5,512IU; Vitamin D
3, 2,200IU; Vitamin E, 6.1IU; Vitamin B, 12,276ug; Riboflavin, 5.5mg; D-pantothenic acid, 13.8mg; Niacin, 30.3mg; Cholinechloride, 551mg; Mn, 100mg; Fe, 100mg; Ze, 100mg; Cu, 234mg; I, 1.4mg; Se, 0.3mg; Co, 1.0mg
2Animal feed is sneaked in the premix material
3Measured value
1.4 feeding and management
Test in peace pig farm, Beijing and carry out, raised a pig free choice feeding and drinking-water 28 days.Temperature is 18 ℃ in the house, humidity 55%.Every circle has independent crib and water fountain.Weigh weekly and ramming material to calculate feed conversion rate.Test the 7th day and the 28th day, to get a pig for every group and carry out rectum and adopt excrement, the oven dry pulverizing is standby.
1.5 lab analysis
The conventional analysis of feedstuff and excrement sample is carried out assay determination according to the method that AOAC (1990) is provided.Aminoacid is measured with automatic amino acid analyzer.
1.6 data statistic analysis
Test data carries out analyzing and processing according to Duncan ' s multiple comparisons with SPSS6.0 (U.S. SPSS Inc.) software.
2 results
Table 2 enteron modifying agent of the present invention is to the influence of piglet fertility performance
As can be seen from Table 2, matched group, the average daily gain of animal feed group 1 and animal feed group 2 is respectively 0.67,0.74 and 0.69kg; Average daily ingestion amount is 1.36,1.32 and 1.38kg; Feed conversion rate is 2.04,1.91 and 2.06.Although the daily gain of full phase animal feed group 1 has improved 10% than matched group, statistical discrepancy is remarkable (p＞0.05) not.
Table 3 enteron modifying agent of the present invention is to the influence of feedstuff apparent digestibility
Annotate: with the female no identical person's significant difference (p＜0.05) of line data shoulder marking-up
Table 4 enteron modifying agent of the present invention is to the influence (Day 28) of feed amino acid apparent digestibility
Annotate: with the female no identical person's significant difference (p＜0.05) of line data shoulder marking-up.
By table 3 and table 4 as can be seen, the 7th day measured feed ingredient digestibility significant difference (p＜0.05) of test, at the 28th day, the apparent digestibility significant difference (p＜0.05) of Organic substance, crude protein and total energy.In addition, the digestibility utmost point of animal feed group 1 calcium phosphorus is higher than matched group and α-animal feed group 2 (p＜0.01) significantly.In addition, 1% enteron modifying agent that adds in the daily ration can make amino acid whose digestibility raising (p＜0.05) in the daily ration.
3 brief summaries
Add suitable enteron modifying agent in the daily ration, can improve the fertility performance of feed digestibility and piglet.
Experimental example 2
This experimental example relates to the influence of enteron modifying agent to the fertility performance of broiler.
1 materials and methods
1.1 experimental animal and feeding and management
Select 360 of 1 close age in days healthy AA broiler chicks of birth weight (public young) for use, be divided into 5 groups at random, 72 every group after stamping wing number.With reference to NRC (1994) broiler nutritional need, be basal diet with the corn-soybean meal diet, divided for 0～3 age in week, 4～6 two stages of age week raised.The experimental basis daily ration is formed and trophic level sees Table 5.
Test chicken adopts ladder to raise in cages 3 in every cage.First week warmed oneself with infrared lamp, and room temperature is controlled at 32～34 ℃, reduced by 2～3 ℃ later on weekly.0～3 week illumination every day 24h, 4～6 week illumination every day 20h.7d and 14d collunarium, eye dripping respectively pass and the immunity of Newcastle disease attenuated Seedling and fabricius bursa, and 21d and 28d drink water respectively, and immunity passes and Newcastle disease attenuated Seedling bigeminy Seedling and infectious bursa of Fabricius.Enteron modifying agent is fed with the powder form, test chicken free choice feeding and drinking-water.
1.2 EXPERIMENTAL DESIGN
The design of single-factor randomized block experiment is adopted in this test, and test is divided into 5 processing, i.e. basal diet (animal feed composition level of the present invention is 0), 1,3,6 and 10g/kg enteron modifying agent of the present invention, and each handles 8 repetitions, and each repeats 9 chickens.
1.3 sample collecting
Write down the animal dead number every day, weigh simultaneously, ramming material.Is that unit claims chicken weight respectively at 21d and 42d to repeat, and ramming material calculates the weightening finish of enteron modifying agent consumption and animal.
1.4 sample analysis
The assay determination of the conventional nutrient of daily ration is carried out according to AOAC (1995) method.Amino acid content uses automatic amino acid analyzer (Hitachi L-8800 in the enteron modifying agent, Japan) measure, most of amino acid whose mensuration are used 6mol/L HCl acid hydrolysis process, sulfur-containing amino acid (methionine and cystine) carries out pre-treatment (GB/T 15399-94) with the oxydrolysis program, tryptophan uses basic hydrolysis (4mol/L LiOH) back to measure (Shimadzu LC 10, Japan) on high performance liquid chromatography.The mensuration of fatty acid is upward carried out quantitatively as interior mark with C19:0 at gas chromatograph (HP 6890, the U.S.) with reference to Sukhija and Palmquist (1988) method.The mensuration of total energy is used oxygen bomb calorimetric instrument (Parr 1281, the U.S.).
Table 5 experimental basis daily ration is formed and trophic level (%)
1Premix material provides for every kilogram of complete feedstuff: vitamin A, 9000IU; Vitamin D
3, 1850IU; Vitamin E, 18IU; Vitamin K, 2.0mg; Vitamin B
1, 1.0mg; Riboflavin, 7.0mg; Pantothenic acid, 15.0mg; Vitamin B
6, 3.0mg; Vitamin B
12, 0.01mg; Nicotinic acid, 36.0mg; Choline chloride, 300mg; Folic acid, 0.60mg; Biotin, 0.10mg; Manganese, 80.0mg; Ferrum, 75.0mg; Zinc, 60.0mg; Copper, 4.0mg; Iodine, 0.4mg and selenium, 0.3mg.
2Measured value
1.5 data statistics
With the SPSS9.0 statistical software every data are carried out variance analysis and multiple comparisons.
2 results and discussion
2.1 enteron modifying agent of the present invention is to the influence of meat chicken production performance
Enteron modifying agent of the present invention sees Table 6 to the weightening finish of the average daily ingestion amount of broiler (ADFI), average daily gain (ADG) and enteron modifying agent than the influence of (F/G) etc.In 0～3 week, the ADG that adds 6g/kg enteron modifying agent group of the present invention is higher than other group, and the ADG of 3g/kg enteron modifying agent is minimum, and two groups of significant differences (P＜0.05); The situation of change of each test group enteron modifying agent transformation efficiency is identical with the variation of ADG, and promptly 6g/kg enteron modifying agent group F/G is minimum, and F/G is the highest for 3g/kg enteron modifying agent group, two groups of significant differences (P＜0.05).In 4～6 weeks, F/G increases and reduces along with the enteron modifying agent addition, and the F/G of 6g/kg and 10g/kg enteron modifying agent group significantly is lower than matched group (P＜0.05).In 0～6 week, the F/G that adds 6g/kg and 10g/kg enteron modifying agent group significantly is lower than matched group (P＜0.01).
Table 6 enteron modifying agent group of the present invention is to the influence of meat chicken production performance
A, bDifferent persons represent significant difference (P＜0.05) with column data shoulder mark
*The average mistake
Enteron modifying agent has obtained the confirmation of most of correlational studyes to the growth promoting function of some aquatic animals.Great majority research thinks that enteron modifying agent can promote broiler growth, increases chest muscle weight and improve efficiency of feed utilization, reduces mortality rate (Lignell etc., 1998 that the yolk sac inflammation causes; Inborr, 1998).In this research, adding 1～10g/kg enteron modifying agent of the present invention does not make significant difference to ADFI and the ADG that tests each phase broiler, but add the feed efficiency that 6g/kg and 10g/kg enteron modifying agent of the present invention can significantly improve test latter stage and full phase broiler, promptly improve 3.8% (P＜0.01) (table 6) than matched group feed efficiency.
3 brief summaries
Add enteron modifying agent of the present invention in the daily ration and can improve the feed efficiency of test latter stage and full phase broiler, 6g/kg and 10g/kg enteron modifying agent of the present invention to improve effect obvious, feed efficiency improves 3.8% than matched group.
Experimental example 3
This experimental example relates to the influence of enteron modifying agent of the present invention to the fertility performance of laying hen.
1 materials and methods
1.1 test site
Beijing China is the pay uncle of exit of valley fowl industry Co., Ltd chicken house all.
1.2 EXPERIMENTAL DESIGN and test daily ration
Select 1275 of the blue brown laying hens in 49 seas in age in week, be divided into 5 groups at random, test is established positive matched group (B, D, E), negative matched group (C) and is added 0.05% animal feed test group (A) (seeing Table 7).Establish 3 repetitions for every group, two are repeated 92 chickens, and one is repeated 91 chickens.Enteron modifying agent of the present invention is provided by the Beijing LongKe FangZhou Biology Engineering Technology center, and M100, NW, GEK are provided by certain producer respectively, is the compound micro-ecological preparation product.
Table 7 test packet design
Corn-bean cake type powdery daily ration is adopted in test, and prescription and trophic level see Table 8.
Table 8 test daily ration and trophic level
Annotate: A, B, D, E group are added enteron modifying agent, M100, NW, GEK respectively in premix material.
1.3 feeding and management
Test and carry out trial test, preliminary trial period 30d on October 1st, 2006.Formally begin test on November 1st, 2006.Raise in cages in the test chicken slope.Day feed 2 times, free choice feeding, inventory with every day hopper show a small surplus and exceed, nipple is freely drunk water.Duration of test is normally prevented epidemic, and the same time of every day is picked up egg.Evening light filling, constant 17h illumination.
1.4 test index
Write down average egg weight, feed intake, egg number, broken egg number every day.During off-test, statistics is respectively organized average egg weight, average laying rate, average egg qualification rate and feedstuff-egg ratio.
1.5 date processing
Test data adopts SPSS11.5 software to carry out SLD variance significance analysis after the EXCEL preliminary treatment.
2 results and analysis
Different additive sees Table 3 to the influence of laying hen fertility performance.
Table 9 different additive influences the result to the fertility performance of laying hen
Annotate: the alphabetical group significant difference inequality of a, b mark representative in the table, alphabetical identical group difference is not remarkable; C, d representative is the same in the table.
By table 9 as seen, average laying rate of A group (enteron modifying agent interpolation group of the present invention) and average egg weight are all higher than positive and negative matched group, and significant difference (p＜0.05) behind the statistical analysis.The broken egg situation of each group of duration of test is few, and average egg qualification rate does not all have significant difference (p＞0.05).A group feedstuff-egg ratio numerical value is obviously all low than all the other each groups.As seen the effect of enteron modifying agent performance in the laying hen material is remarkable.
3 discuss and conclusion
3.1 test from this, interpolation enteron modifying agent of the present invention is more remarkable than adding single probiotics preparation effect in the laying hen material, except the E group, because of its trophic level is lower than other four groups of diets of different nutrition level, does not compare on same foundation certainly.
3.2 this test explanation, present Chinese laying hen prescription practical situation, should the reasonable new raw material of selectivity price ratio basis on, must consider the performance of like product.
3.3 from three indexs, average egg qualification rate zero difference is remarkable, shows that microbial ecological agent has obvious effect for the qualification rate of egg, has illustrated that microbial ecological agent is remarkable for the flora regulating effect of Intestinum Gallus domesticus road and urinary system; And the significant difference result of laying rate and egg size has shown that microbial ecological agent is not only powerful from regulating on the interior environment, and different products stress be gone up difference to some extent for the raising and the immunity raising minimizing of diets of different nutrition level.
The effect of embodiment 4 enteron modifying agents in ablactational baby pig produces
This experimental example relates to the influence of enteron modifying agent of the present invention to the fertility performance of ablactational baby pig.
1 test material and method
1.1 test objective
Verify that input safe in utilization substitutes antibiotic in the daily ration to the influence of ablactational baby pig fertility performance, for its application in production practices provides reference data.
1.2 experimental animal grouping and test daily ration are formed
28 age in days Du * length * Dasanyuan hybridization 72 of ablactational baby pig (male and female half and half) are selected in test for use, are divided into 3 processing at random by body weight, sex, the similar principle of hereditary basis, and each handles 4 repetitions (hurdle), each 2 hurdle of male and female, 6 on every hurdle.The test processed group is respectively negative matched group (antibiotic-free), positive matched group (added with antibiotic chlortetracycline 0.05%, A Meila mycin 0.02%) and test group (adding enteron modifying agent of the present invention), and EXPERIMENTAL DESIGN sees Table 10.
Experimental period is 35 days, tests grouping, ear tag numbering before the examination phase.The disposable interpolation of enteron modifying agent of the present invention, addition are 0.1%; Enteron modifying agent of the present invention is provided by " Beijing LongKe FangZhou Biology Engineering Technology center ", and the test daily ration is based on total amino acids, with reference to NRC (1998) pig nutritional need recommended levels preparation basal diet.The composition and the nutritional labeling of basal diet see Table 11.
Table 10 EXPERIMENTAL DESIGN
| The test processed group | The premix material numbering | Handle repeat number | Every repetition pig number |
| Negative matched group (A) | 1 | 4 | 6 |
| Positive matched group (B) | 2 | 4 | 6 |
| Enteron modifying agent group of the present invention (C) | 3 | 4 | 6 |
1.3 feeding and management
Test is carried out on gold prospect pig farm, Beijing.A test pig free choice feeding, drinking-water, but enteron modifying agent can not add volume, in case contamination and deterioration.Anthelmintic immunity, disinfectant program carry out according to the pig farm routine.
1.4 test detects index
The 1st day examination phase and the 35th day only carry out individuality to test pig respectively and weigh; Write down the mensuration of feed consumption rate (is unit with every hurdle), diarrhoea pig's head number and immunologic cellular activity in the process of the test, calculate average daily gain, average daily ingestion amount and indexs such as feed efficiency (material anharmonic ratio) and diarrhea rate.
1.5 statistical analysis
Total data input computer carries out statistical analysis with the SPSS12.0 statistical software to data.
Table 11 piglet basal diet is formed and nutritional labeling
Premix material 1: antibiotic-free; Premix material 2: add antibiotic; Premix material 3: add enteron modifying agent
2. result of the test and analysis
2.1 fertility performance
As can be seen from Table 12, the average daily gain of enteron modifying agent group piglet of the present invention exceeds 1.5% and 3.6% respectively than antibiotic group and antibiotic-free group, and difference is remarkable (P＞0.05) not; Feedstuff-meat ratio is respectively than other two groups low 3.01% (resist) and 4.40% (nonreactive), but difference not remarkable (P＞0.05); Aspect diarrhea rate, enteron modifying agent group of the present invention is than antibiotic group height, but is lower than antibiotic-free compositions group, and three's difference is significantly (P＞0.05) not; Survival rate aspect enteron modifying agent and antibiotic group all exceed 4.3% than antibiotic-free group.From whole test, the fertility performance of enteron modifying agent group piglet is better than other two groups.
Table 12 enteron modifying agent of the present invention is to the influence of piglet fertility performance
| The enteron modifying agent group | The antibiotic group | The antibiotic-free group | |
| Average daily gain (g) | 595 | 586 | 574 |
| Feedstuff-meat ratio | 1.94 | 1.99 | 2.03 |
| Diarrhea rate (%) | 0.61 | 0.56 | 0.77 |
| Survival rate (%) | 100 | 100 | 95.8 |
2.2 Economic and Efficiency Analysis
In table 13 we as can be seen, under the identical situation of expenses such as, water power artificial at other, the hair of every piglet of enteron modifying agent group of the present invention is overcharged into 5.83 yuan and 14.10 yuan better economic benefit than antibiotic group.
Table 13 Economic and Efficiency Analysis
| Enteron modifying agent group of the present invention | The antibiotic group | The antibiotic-free group | |
| Total feed consumption (kg) | 932.5 | 947.9 | 938.1 |
| Total augment weight (kg) | 481.4 | 476.5 | 461.7 |
| Feedstuff always drops into (unit) | 1986.23 | 2037.99 | 1970.01 |
| Total output that increases weight (unit) | 8665.2 | 8577.00 | 8310.60 |
| Every capillus profit (unit) | 278.29 | 272.46 | 264.19 |
| The feedstuff unit price (unit/kg) | 2.13 | 2.15 | 2.10 |
| The piglet valency (unit/kg) | 18.00 | 18.00 | 18.00 |
3 conclusions
Enteron modifying agent among the present invention is as the compositions of a kind of bacillus cereus and Chinese herbal medicine extract, and main effect is to regulate the animal intestinal environment, improves animal body immunity, and then promotes the raising of breeding performonce fo animals.Can be used as safe input by this evidence enteron modifying agent and in piglet produces, use, and the raising of growth performance and economic benefit are better than antibiotic.
More than given embodiment be in order to further specify the specific embodiments of the invention scheme, feature of the present invention, advantage and other details are not to be used for limiting protection scope of the present invention; Any variation of being done in the present invention's design all should be among scope of the present invention.
Claims (10)
1. enteron modifying agent is characterized in that described enteron modifying agent comprises:
Contain culture of strains liquid: 13～50 parts;
1～9 part of Chinese herbal medicine extract, wherein said Chinese herbal medicine are a kind of in the Radix Astragali, Aloe, Fructus Lycii, Herba Houttuyniae, Herba Portulacae or the Ramulus Uncariae Cum Uncis;
Adjuvant: 45～85 parts;
Described strain is bacillus cereus (Bacillus), is called the BB bacterial strain, and preserving number is CGMCC No.1007.
2. regulator according to claim 1 is characterized in that adjuvant is a kind of in precipitated calcium carbonate, bean cake, Testa Tritici, the fish flour, a kind of in preferred precipitated calcium carbonate, bean cake, the Testa Tritici.
3. regulator according to claim 1 is characterized in that described Chinese herbal medicine is a kind of in Aloe, Herba Houttuyniae, Herba Portulacae or the Ramulus Uncariae Cum Uncis.
4. regulator according to claim 1 is characterized in that described culture fluid is the glucose nutrient broth.
5. regulator according to claim 1 is characterized in that described Chinese herbal medicine extract is to obtain by the following method:
A. a kind of in the described Chinese herbal medicine sheared, cleans, obtain pending natural Chinese medicinal herb plant;
B. pending natural Chinese medicinal herb is distilled with water vapour, and the Chinese herbal medicine residue that obtains after the distillation is merged;
C. the Chinese herbal medicine residue water that will obtain after will merging decocts and extracts, and obtains extracting solution;
D. the precipitate that obtains wetting with the described extracting solution of 85% ethanol precipitation;
E. described wet precipitate is carried out spray drying and obtain described Chinese herbal medicine extract.
6. a method for preparing the described enteron modifying agent of claim 1 is characterized in that, described regulator prepares by following method:
A. the BB inoculation of described bacillus cereus is cultivated in culture medium, as inoculum;
B. then described inoculum is inoculated in another kind of culture medium, and adds in the described Chinese herbal medicine extract and cultivate, make culture fluid;
C. provide oxygen to described culture fluid, the culture fluid of described interpolation oxygen is incubated, and stirs simultaneously;
D. in the culture fluid that finishes insulation, add described adjuvant, mix;
E. mixed culture fluid is dried, and pulverizes promptly to get described enteron modifying agent.
7. method according to claim 6 is characterized in that, the culture fluid of described interpolation oxygen is incubated 20～40 hours under 30～40 ℃ of temperature, and the speed that stirs is 60～100 rev/mins.
8. method according to claim 6, the condition of culture of BB bacterial strain in culture medium that it is characterized in that described bacillus cereus cultivated 3 days in 37 ℃ of calorstats.
9. method according to claim 6 is characterized in that: described another kind of culture medium is the glucose nutrient broth, and it is to be grouped into by following one-tenth: peptone 10g, Carnis Bovis seu Bubali cream 5g, sodium chloride 5g, glucose 2.5g, agar 15g, distilled water 1000ml.
10. enteron modifying agent according to claim 1 is characterized in that described enteron modifying agent is used to regulate the colony balance of animal alimentary canal, control intestinal balance.
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| CNA2007100905456A CN101244184A (en) | 2007-04-11 | 2007-04-11 | Enteron modifying agent, preparation method and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102578384A (en) * | 2011-04-01 | 2012-07-18 | 北京都润科技有限公司 | Application of bacillus subtilis and probiotic synergist to synbiotic fermentation |
| CN103168960A (en) * | 2013-03-05 | 2013-06-26 | 中山大学 | Composite immunity enhancing agent, and preparation method and application thereof |
| CN103627649A (en) * | 2013-05-21 | 2014-03-12 | 郑州牧业工程高等专科学校 | Bacillus subtilis fermentation medium |
| CN104770596A (en) * | 2015-04-07 | 2015-07-15 | 中国水产科学研究院珠江水产研究所 | Aquatic animal intestinal regulator and application thereof |
| CN105767535A (en) * | 2016-03-18 | 2016-07-20 | 刘锦花 | Feed additive for livestocks |
| CN108112776A (en) * | 2017-09-15 | 2018-06-05 | 普乐格（厦门）生物科技有限公司 | It is a kind of for microorganism formulation of animal and bird intestines injury repair and preparation method thereof |
| CN111758852A (en) * | 2020-06-16 | 2020-10-13 | 广东省农业科学院动物科学研究所 | Additive premix for improving intestinal health of broiler chickens and application thereof |
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2007
- 2007-04-11 CN CNA2007100905456A patent/CN101244184A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102578384A (en) * | 2011-04-01 | 2012-07-18 | 北京都润科技有限公司 | Application of bacillus subtilis and probiotic synergist to synbiotic fermentation |
| CN103168960A (en) * | 2013-03-05 | 2013-06-26 | 中山大学 | Composite immunity enhancing agent, and preparation method and application thereof |
| CN103168960B (en) * | 2013-03-05 | 2014-08-06 | 中山大学 | Composite immunity enhancing agent, and preparation method and application thereof |
| CN103627649A (en) * | 2013-05-21 | 2014-03-12 | 郑州牧业工程高等专科学校 | Bacillus subtilis fermentation medium |
| CN104770596A (en) * | 2015-04-07 | 2015-07-15 | 中国水产科学研究院珠江水产研究所 | Aquatic animal intestinal regulator and application thereof |
| CN105767535A (en) * | 2016-03-18 | 2016-07-20 | 刘锦花 | Feed additive for livestocks |
| CN108112776A (en) * | 2017-09-15 | 2018-06-05 | 普乐格（厦门）生物科技有限公司 | It is a kind of for microorganism formulation of animal and bird intestines injury repair and preparation method thereof |
| CN111758852A (en) * | 2020-06-16 | 2020-10-13 | 广东省农业科学院动物科学研究所 | Additive premix for improving intestinal health of broiler chickens and application thereof |
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