CN102578384A - Application of bacillus subtilis and probiotic synergist to synbiotic fermentation - Google Patents
Application of bacillus subtilis and probiotic synergist to synbiotic fermentation Download PDFInfo
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- 235000014469 Bacillus subtilis Nutrition 0.000 title claims abstract description 35
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 30
- 238000000855 fermentation Methods 0.000 title claims abstract description 8
- 230000004151 fermentation Effects 0.000 title claims abstract description 8
- 235000019722 synbiotics Nutrition 0.000 title claims abstract description 8
- 230000012010 growth Effects 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 15
- 239000001963 growth medium Substances 0.000 claims description 13
- 238000002360 preparation method Methods 0.000 claims description 11
- 239000001888 Peptone Substances 0.000 claims description 10
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- 238000000034 method Methods 0.000 claims description 10
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- 241000186000 Bifidobacterium Species 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 238000011156 evaluation Methods 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 6
- 238000011218 seed culture Methods 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- 239000011550 stock solution Substances 0.000 claims description 6
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 5
- 238000009630 liquid culture Methods 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 235000015278 beef Nutrition 0.000 claims description 4
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- 239000008103 glucose Substances 0.000 claims description 4
- 230000035755 proliferation Effects 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 238000004458 analytical method Methods 0.000 claims description 3
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 claims description 3
- 238000007865 diluting Methods 0.000 claims description 3
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- 229920001817 Agar Polymers 0.000 claims description 2
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- 239000008272 agar Substances 0.000 claims description 2
- FYGDTMLNYKFZSV-DZOUCCHMSA-N alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-D-Glcp Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@H](OC(O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-DZOUCCHMSA-N 0.000 claims description 2
- 230000003698 anagen phase Effects 0.000 claims description 2
- 239000000605 aspartame Substances 0.000 claims description 2
- 229960003438 aspartame Drugs 0.000 claims description 2
- 235000010357 aspartame Nutrition 0.000 claims description 2
- -1 aspartame oligosaccharide Chemical class 0.000 claims description 2
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 claims description 2
- 229940107187 fructooligosaccharide Drugs 0.000 claims description 2
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
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- 239000003674 animal food additive Substances 0.000 description 2
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- 238000011160 research Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to the application of bacillus subtilis and a probiotic synergist to synbiotic fermentation and provides the probiotic synergist aiming at special bacillus subtilis. The probiotic synergist is prepared by the following steps: performing synbiotic fermentation by using the probiotic synergist and the bacillus subtilis; selecting the probiotic synergists of different concentrations to conduct an experiment; and estimating the produced maximum production capacity K value and a generation time G to obtain the required probiotic synergist at proper concentration, therefore, the generation time of the bacillus subtilis can be effectively shortened and the maximum growth amount can be improved.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to application of bacillus subtilis in combination with a probiotic synergist for synbiotic fermentation.
Background
Since the discovery that the feed antibiotics can play a role in promoting growth by influencing the initial colonization of intestinal microorganisms in a host, the application of the antibiotics generates great economic benefits in the field of animal husbandry. However, due to the large use of antibiotics, the negative effects are increasingly prominent, which are shown in that pathogenic microorganisms generate drug resistance and drugs are remained in animal products, thereby causing potential harm to the health of human beings. Therefore, research and development of antibiotic substitute probiotics, enzyme preparations, acid preparations, and the like have been hot spots for green feed additives in recent years, and among them, probiotics are receiving attention. As one of the probiotics, the role of bacillus subtilis preparation in animal production is a focus of attention of research and production personnel in animal husbandry.
The bacillus subtilis preparation is the most important type of microecological preparation, can promote digestion and absorption of nutrition, generates substances such as organic acid and the like to inhibit propagation of intestinal putrefying bacteria and pathogenic bacteria, inhibits intestinal infection, relieves diarrhea, and has the functions of immunocompetence and the like.
As one of probiotics, the bacillus subtilis has the characteristics of strong stress resistance, high temperature and high pressure resistance, easiness in storage and the like, and has a focus of attention of scientific research and production personnel in animal husbandry in animal production. However, the strains produced by microecological preparations in the feed industry in China are relatively laggard, and more strains with the characteristics of strong fertility, stable performance and the like are urgently needed to be screened for production and application.
In order to solve the problems of probiotics, the probiotics synergist is used for promoting the proliferation and the strain stability of the bacillus subtilis. Currently, in the field of microbial feed additives, probiotic synergists mainly comprise substances such as oligosaccharides and polysaccharides.
Disclosure of Invention
The invention aims to provide a probiotic synergist for specific bacillus subtilis (with the number of CGMCC 1007), perform synbiotic fermentation by utilizing the probiotic synergist and the bacillus subtilis, perform experiments by selecting probiotic synergists with different concentrations, and evaluate the generated maximum production K value and the generation G value to obtain the required probiotic synergist with proper concentration, so that the generation time of the bacillus subtilis can be effectively shortened and the maximum growth amount can be increased.
To achieve the purpose of the invention, the specific Bacillus subtilis is deposited in Bacillus subtilis of China general microbiological culture Collection center at 1 st 4 th 2008, and the registration number of the collection center is as follows: CGMCC No. 1007.
The Bacillus subtilis CGMCC No.1007 is a gram-positive Bacillus and a facultative anaerobe, and a single cell is 0.7-0.8 multiplied by 2-3 microns, is uniformly colored, has no capsule, is a periphytic flagellum, and can move. Gram-positive bacteria, spores of 0.6-0.9 multiplied by 1.0-1.5 microns, oval to columnar shape, central or slightly deviated, and the bacteria do not expand after the spores are formed. The colony surface is rough and opaque, and is dirty white or yellowish.
Probiotic synergists refer to substances that selectively stimulate the growth of one or more bacteria in the host's intestinal tract, improving the health of the host without being digested by the host's gastrointestinal tract. The bifidobacterium sugar is selected as the probiotic synergist of the bacillus subtilis.
Detailed Description
The present invention will now be described in detail with reference to examples, which are intended to be illustrative, but not limiting, of the invention.
Preparing the bacillus subtilis seed solution for inoculation: bacillus subtilis (CGMCC No. 1007) is inoculated in a slant culture medium, and the formula of the slant culture medium is as follows: culturing 10g of peptone, 5g of beef extract, 5g of sodium chloride, 2.5g of glucose, 15g of agar and 1000ml of distilled water in a thermostat at 37 ℃ for 72 hours to obtain a seed culture; the seed culture is inoculated into a glucose liquid culture medium, the formula of the glucose liquid culture medium comprises 10g of peptone, 5g of beef extract, 5g of sodium chloride, 2.5g of glucose and 1000ml of distilled water, and the temperature is kept constant for 24 hours at the temperature of 37 ℃.
Preparation of probiotic standard curve: inoculating a certain amount of the above seed solution into a 150ml triangular flask (containing a certain amount of peptone water, such as 50ml), placing the triangular flask into a 37 deg.C water bath oscillator, and culturing at 90rpm for 10 hr to obtain stock solution; diluting the stock solution according to the proportion of 0%, 20%, 50%, 60%, 80% and 100%, respectively carrying out color comparison under the condition that the wavelength is 660nm, counting the number of bacteria by using a flat plate counting method, calculating an average value, measuring and recording according to the relation between the number of bacteria and an OD value, repeating for four times, then carrying out variance analysis, and calculating a regression equation.
Evaluation of probiotic synergist bifidobacterium sugar on the K value and the G value of the maximum production of bacillus subtilis: collecting (68.46g/L) of Bifidobacterium sugar mother liquor (subjected to filtration sterilization), adding into peptone water culture medium at working concentrations of 0, 2.63, 1.34, 0.68, and 0.14g/L (control, concentration 1, concentration 2, concentration 3, and concentration 4), mixing, placing into a water bath oscillator, and standing at 37 deg.C and 90rpm for 12 hr; the number of bacteria was measured by plate counting at 0 hour, OD (wavelength 660nm) was measured at 6, 8, 10 and 12 hours, the corresponding number of bacteria was obtained by substituting the standard curve equation, and the Logistic proliferation curve was calculated by 3-point method to obtain the K value (i.e., the maximum growth). Then selecting any two points in logarithmic growth phase, and fitting in formula G ═ LN (N)t)-LN(N0)/(Tt-T0) And the generation time (G value) is calculated. And performing multiple comparison on the K value and the G value by using an LSD method.
Probiotic synergist bifidosaccharide on bacillus subtilis (K10)8cfu/ml) value, G (min) value:
the K, G values were analyzed for variance using the LSD method. The result shows that different concentrations of the probiotic synergist such as the bifidobacterium sugar have different degrees of influence on the Bacillus subtilis, and the higher the concentration of the probiotic synergist in the concentration range, the more obvious the effect is.
The four experimental concentrations of the bifidobacterium sugar can remarkably or remarkably shorten the generation time of the bacillus subtilis and improve the maximum growth amount, and the concentration of 4 is the best in terms of action effect, but the application value of low concentration (concentration 1) is the greatest in consideration of comprehensive use effect and benefit. The bifidus sugar is a mixture of isomaltooligosaccharide, fructooligosaccharide, maltooligosaccharide, aspartame, etc., and a dipeptide sweetener.
The inventor combines the probiotic synergist of the bifidobacterium sugar with the bacillus subtilis to carry out synbiotic fermentation, and tests prove that the generation time of the bacillus subtilis is shortened and the maximum growth amount of the bacillus subtilis is increased.
Claims (8)
1. The application of bacillus subtilis and a probiotic synergist for synbiotic fermentation is characterized in that: evaluations were performed using different concentrations of probiotic synergists, including evaluations of the K value for the maximum growth and the G value for the generation of bacillus subtilis, thereby increasing the maximum growth and shortening the generation.
2. The use of claim 1, wherein: before the evaluation of the maximum growth K value, the inoculation preparation of the bacillus subtilis seed solution and the preparation of a probiotic standard curve are also included.
3. The use of claim 1, wherein: before the evaluation of the generation time G value, the preparation of inoculation of the bacillus subtilis seed solution and the preparation of a probiotic standard curve are also included.
4. Use according to claim 2, characterized in that: wherein,
the preparation of the bacillus subtilis seed solution inoculation is as follows:
inoculating bacillus subtilis into a slant culture medium, and culturing for 72 hours at the temperature of 37 ℃ in a thermostat to obtain a seed culture; inoculating the seed culture into a glucose liquid culture medium, and keeping the temperature at 37 ℃ for 24 hours;
the probiotic standard curve was prepared as follows:
inoculating a certain amount of the seed liquid and a certain amount of peptone water into a 150ml triangular flask, putting the triangular flask into a 37 ℃ water bath oscillator, and culturing for 10 hours under the condition of 90rpm to obtain a stock solution; diluting the stock solution according to the proportion of 0%, 20%, 50%, 60%, 80% and 100%, performing colorimetry under the condition that the wavelength is 660nm, counting the number of bacteria by using a flat plate counting method, calculating an average value, measuring and recording according to the relation between the number of bacteria and an OD value, repeating for four times, then performing variance analysis, and calculating a regression equation;
evaluation of the maximum growth amount K value:
taking 68.46g/L standard bifidobacterium sugar mother liquor as a probiotic synergist, adding the working concentrations of 0, 2.63, 1.34, 0.68 and 0.14g/L into peptone water culture medium, wherein the working concentrations are respectively set as control, concentration 1, concentration 2, concentration 3 and concentration 4, fully mixing, putting into a water bath oscillator, and standing at 37 ℃ and 90rpm for 12 hours; and measuring the number of bacteria by using a plate counting method at 0 hour, measuring OD values at 6, 8, 10 and 12 hours, measuring the wavelength to be 660nm, substituting the standard curve equation to obtain the corresponding number of bacteria, and calculating a Logistic proliferation curve by using a 3-point method to obtain the maximum growth K value.
5. Use according to claim 3, characterized in that: wherein,
the preparation of the bacillus subtilis seed solution inoculation is as follows:
inoculating bacillus subtilis into a slant culture medium, and culturing for 72 hours at the temperature of 37 ℃ in a thermostat to obtain a seed culture; inoculating the seed culture into a glucose liquid culture medium, and keeping the temperature at 37 ℃ for 24 hours;
the probiotic standard curve was prepared as follows:
inoculating a certain amount of the seed liquid and a certain amount of peptone water into a 150ml triangular flask, putting the triangular flask into a 37 ℃ water bath oscillator, and culturing for 10 hours under the condition of 90rpm to obtain a stock solution; diluting the stock solution according to the proportion of 0%, 20%, 50%, 60%, 80% and 100%, performing colorimetry under the condition that the wavelength is 660nm, counting the number of bacteria by using a flat plate counting method, calculating an average value, measuring and recording according to the relation between the number of bacteria and an OD value, repeating for four times, then performing variance analysis, and calculating a regression equation;
evaluation of the epoch G value:
taking 68.46g/L standard bifidobacterium sugar mother liquor as a probiotic synergist, adding the working concentrations of 0, 2.63, 1.34, 0.68 and 0.14g/L into peptone water culture medium, wherein the working concentrations are respectively set as control, concentration 1, concentration 2, concentration 3 and concentration 4, fully mixing, putting into a water bath oscillator, and standing at 37 ℃ and 90rpm for 12 hours; measuring the number of bacteria by using a flat plate counting method in 0 hour, measuring OD values in 6, 8, 10 and 12 hours, measuring the wavelength to be 660nm, substituting the standard curve equation to obtain the corresponding number of bacteria, and calculating a Logistic proliferation curve by using a 3-point method to obtain a maximum growth K value; then any two points in the logarithmic growth phase are selected and sleeved into a formula to obtain the generation time G value.
6. Use according to claim 4 or 5, characterized in that: the formula of the slant culture medium is as follows: 10g of peptone, 5g of beef extract, 5g of sodium chloride, 2.5g of glucose, 15g of agar and 1000ml of distilled water.
7. Use according to claim 4 or 5, characterized in that: the formula of the glucose liquid culture medium comprises 10g of peptone, 5g of beef extract, 5g of sodium chloride, 2.5g of glucose and 1000ml of distilled water.
8. Use according to claim 4 or 5, characterized in that: the said bifidus sugar is a mixture of isomaltooligosaccharide, fructo-oligosaccharide, maltooligosaccharide, aspartame oligosaccharide and a dipeptide sweetener.
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| CN2011100808170A CN102578384A (en) | 2011-04-01 | 2011-04-01 | Application of bacillus subtilis and probiotic synergist to synbiotic fermentation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114081097A (en) * | 2022-01-10 | 2022-02-25 | 北京都润科技有限公司 | Application of probiotic synergist in microbial fermentation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101244184A (en) * | 2007-04-11 | 2008-08-20 | 北京龙科方舟生物工程技术中心 | Enteron modifying agent, preparation method and application thereof |
| CN101803681A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Oligopolymerization chitosan-astragalus polysaccharide ecological feed additive |
| CN101805710A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Culture medium of plant extract radix astragali polysaccharide complex microorganism |
| CN101812419A (en) * | 2009-12-30 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Epimedium extract-oligomeric chitosan composite probiotic colony culture medium |
-
2011
- 2011-04-01 CN CN2011100808170A patent/CN102578384A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101244184A (en) * | 2007-04-11 | 2008-08-20 | 北京龙科方舟生物工程技术中心 | Enteron modifying agent, preparation method and application thereof |
| CN101803681A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Oligopolymerization chitosan-astragalus polysaccharide ecological feed additive |
| CN101805710A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Culture medium of plant extract radix astragali polysaccharide complex microorganism |
| CN101812419A (en) * | 2009-12-30 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Epimedium extract-oligomeric chitosan composite probiotic colony culture medium |
Non-Patent Citations (1)
| Title |
|---|
| 孙鸣等: "益生芽孢杆菌与益生协同剂在体外相互关系研究", 《饲料研究》, no. 1, 31 January 2009 (2009-01-31), pages 70 - 71 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114081097A (en) * | 2022-01-10 | 2022-02-25 | 北京都润科技有限公司 | Application of probiotic synergist in microbial fermentation |
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Application publication date: 20120718 |