CN111758852A - Additive premix for improving intestinal health of broiler chickens and application thereof - Google Patents
Additive premix for improving intestinal health of broiler chickens and application thereof Download PDFInfo
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Abstract
The invention discloses an additive premix for improving intestinal health of broiler chickens and application thereof, and belongs to the technical field of poultry feeds. The broiler feed additive premix consists of an additive and a carrier; wherein the additive is prepared by mixing arginine, threonine, tryptophan, isoleucine, vitamin E, curcumin, essential oil, polysaccharide, bacillus subtilis and clostridium butyricum; the carrier is corncob powder, zeolite powder or bentonite. The additive can improve oxidation resistance and digestion capacity of yellow-feathered broilers, promote growth and reproduction of beneficial bacteria in intestinal tracts, reduce oxidative stress of organisms, inhibit growth of harmful bacteria in intestinal tracts, reduce the levels of ammonia gas and bacterial toxins in the intestinal tracts, and has the effects of promoting growth and improving oxidation resistance and immune function of the intestinal tracts of the yellow-feathered broilers. The additive premix disclosed by the invention is green and safe, does not contain antibiotics, can improve the intestinal oxidation resistance and the immune function of yellow-feathered broilers, is beneficial to the healthy and sustainable development of the breeding industry and the feed industry, and is suitable for popularization and application in the market.
Description
Technical Field
The invention belongs to the technical field of poultry feed, in particular relates to an additive premix for improving intestinal health of broiler chickens and application thereof, and particularly relates to an antibiotic-free feed additive premix for improving intestinal health of yellow-feather broiler chickens and application thereof in preparation of feeds for yellow-feather broiler chickens such as Linnan yellow-feather broiler chickens, Qingyuan broilers and beard chickens.
Background
In recent years, the broiler breeding industry in China is rapidly developed, and chicken becomes the second largest meat consumer product next to pork in China. The yellow-feather broiler industry in south China, which takes two Guangdong countries as the center, is also rapidly developed, and the safe and efficient production of the yellow-feather broilers has important significance for enriching the content of animal products and improving the living standard of people. But the immunity of the yellow-feathered broilers is obviously reduced because the production performance of the yellow-feathered broilers is improved by modern genetic breeding selection at present. In the production of broiler chickens, intestinal diseases caused by virus, bacterial infection, mycotoxin, severe feeding environment and the like are very common, and antibiotics, chemical drugs and the like are frequently used, so that not only is great economic loss caused, but also difficulty is brought to the treatment of livestock and poultry, and the health of human bodies is harmed. Meanwhile, relevant laws are formulated and implemented by rural parts of agriculture, and antibiotics are prohibited from being added to the feed comprehensively in 2020. Therefore, the search for green, safe and environment-friendly feed additives to replace antibiotics has become one of the important directions of current feed research.
At present, in intensive breeding of modern broilers, yellow-feather broiler feed is mainly corn/wheat/sorghum-soybean meal-miscellaneous meal type feed, the composition of amino acids is unbalanced, and the lack of functional amino acids such as arginine and threonine affects intestinal development, so that intestinal barrier function is damaged. Therefore, functional amino acid is reasonably added into the feed in an amino acid balance mode, and meanwhile, the use of antibiotics is reduced by combining the natural plant extract and the probiotics, so that the medicine residue in vivo is reduced, the oxidation resistance and the immunity of the intestinal tracts of yellow-feathered broilers are promoted, and the stabilization of the intestinal flora has very important significance.
In conclusion, the problem to be solved by the technical personnel in the field is how to develop a green, safe and antibiotic-free feed additive premix for improving the intestinal health of broiler chickens.
Disclosure of Invention
In view of the above, the invention aims to provide a green and safe feed additive premix for improving the intestinal health of yellow-feathered broilers without antibiotics aiming at the problems in the prior art, which can relieve the oxidative stress of the intestinal tract, maintain the good growth performance, oxidation resistance and immune function of the yellow-feathered broilers and obviously improve the intestinal health of the broilers.
In order to achieve the purpose, the invention adopts the following technical scheme:
an additive premix for improving intestinal health of broiler chickens, which consists of an additive and a carrier; wherein the content of the first and second substances,
the additive is composed of the following raw materials in addition: 1.0-2.0 g/kg of arginine, 0.6-0.9 g/kg of threonine, 0.2-0.4 g/kg of tryptophan, 0.4-0.6 g/kg of isoleucine, 0.02-0.04 g/kg of vitamin E, 0.15-0.3 g/kg of curcumin, 0.15-0.3 g/kg of essential oil, 0.1-0.2 g/kg of polysaccharide, 0.3-0.6 g/kg of bacillus subtilis and 0.1-0.3 g/kg of clostridium butyricum;
the carrier is corncob powder, zeolite powder or bentonite; and the addition amount of the carrier is 40-60% of the mass of the additive premix.
Further, the above-mentioned additive raw materials are preferably as follows:
the arginine is preferably L-arginine,
the threonine is preferably L-threonine,
the tryptophan is preferably L-tryptophan,
the isoleucine is preferably L-isoleucine,
the essential oil is preferably oregano essential oil, thymol essential oil, carvacrol essential oil, cinnamaldehyde essential oil or tea tree essential oil,
the polysaccharide is preferably mushroom polysaccharide, astragalus polysaccharide or chitosan,
the Bacillus subtilis is preferably a Bacillus subtilis strain,
the clostridium butyricum is preferably clostridium butyricum.
Furthermore, the active ingredient content of the raw materials of the additive is as follows:
the content of arginine in the L-arginine is 98.5 percent;
the threonine content of the L-threonine is 98.5 percent;
the content of threonine in the L-tryptophan is 98 percent;
the content of threonine in the L-isoleucine is 98%;
the effective component content in the curcumin is 98%;
the essential oil contains 5% of effective components of origanum essential oil, thymol essential oil, carvacrol essential oil, cinnamyl phenol essential oil and tea tree essential oil;
the effective component contents of mushroom polysaccharide, astragalus polysaccharide and chitosan in the polysaccharide are 98.5%;
the effective viable count of the bacillus subtilis is 2 × 109cfu/g；
The effective viable count of the clostridium butyricum is 3 × 108cfu/g。
The effective components of the above disclosed additive raw materials have the following effects:
functional amino acids such as arginine, threonine and tryptophan can promote intestinal development and regulate intestinal immune balance; the plant extract curcumin, essential oil and polysaccharide have antibacterial, antioxidant and immunity enhancing effects; the probiotics such as bacillus subtilis, lactobacillus plantarum and the like can inhibit the growth of harmful bacteria in intestinal tracts, promote the growth of beneficial bacteria, regulate intestinal microflora and enhance the immune function.
In addition, the invention also claims the application of the additive premix for improving the intestinal health of the broiler chickens in the preparation of broiler chicken feed.
Specifically, the broiler chicken is yellow-feather broiler chicken such as Lingnan yellow-feather broiler chicken, Qingyuan chicken, beard chicken and the like.
Further, the additive premix is used for preparing the broiler feed, and the using method of the additive premix for improving intestinal health of the broiler is similar to that of other feed additive premixes, the premix is added into 4% of premix feed according to the proportion of 25-50 kg/ton, the premix is added into 20% of concentrated feed according to the proportion of 5-10 kg/ton, and the premix is added into complete formula feed according to the proportion of 1-2 kg/ton.
According to the technical scheme, compared with the prior art, the additive premix for improving the intestinal health of the broiler chicken and the application thereof provided by the invention have the following excellent effects:
(1) the preparation and application methods of the additive premix disclosed by the invention are simple and convenient, and the additive premix has the effects of no antibiotics, no toxic or side effect and no residue;
(2) the additive premix disclosed by the invention can improve intestinal digestion, oxidation resistance and immune functions of broiler chickens, promote the growth and reproduction of beneficial bacteria in intestinal tracts, reduce the oxidative stress of organisms, inhibit the growth of harmful bacteria such as escherichia coli and salmonella in the intestinal tracts, reduce the levels of ammonia gas and bacterial toxins in the intestinal tracts and is beneficial to the health of the intestinal tracts.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a 40X staining micrograph showing jejunal hematoxylin-eosin staining of 30-day-old Qingyuan hen hens in example 1 of the present invention. Wherein (a) is antibiotic control group and (b) is antibiotic-free complete mixed group; (c) is an antibiotic-free functional amino acid reduction group; (d) is antibiotic-free natural plant extract subtraction group; (e) is a probiotic bacteria reduction group without antibiotics.
Detailed Description
The technical solutions disclosed in the present invention will be clearly and completely described below with reference to the drawings and the corresponding embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention discloses a green and safe feed additive premix free of antibiotics and used for improving intestinal health of broiler chickens.
The present invention is further illustrated by the following examples for better understanding, but is not to be construed as limited thereto, and certain insubstantial modifications and adaptations of the invention by those skilled in the art based on the foregoing disclosure are intended to be included within the scope of the invention.
Example 1:
an additive premix without antibiotics for improving intestinal health of broiler chickens, wherein the dosage of additives L-arginine, L-threonine, L-tryptophan, L-isoleucine, vitamin E, curcumin, oregano essential oil, mushroom fungus polysaccharide and bacillus subtilis is respectively 1.0g/kg of L-arginine, 0.6g/kg of L-threonine, 0.2g/kg of L-tryptophan, 0.4g/kg of L-isoleucine, 20mg/kg of vitamin E, 150mg/kg of curcumin, 150mg/kg of oregano essential oil, 100mg/kg of mushroom fungus polysaccharide, 300mg/kg of bacillus subtilis and 100mg/kg of clostridium butyricum, and the carrier of the additive premix is corncob powder, zeolite powder or bentonite.
Finally, the additive premix is added into the Qingyuan partridge chicken feed according to the proportion of 1 kg/ton.
Example 2:
an additive premix without antibiotics for improving intestinal health of broiler chickens, wherein the dosage of additives L-arginine, L-threonine, L-tryptophan, L-isoleucine, vitamin E, curcumin, thymol essential oil, astragalus polysaccharide and bacillus subtilis are respectively 2.0g/kg of L-arginine, 0.9g/kg of L-threonine, 0.4g/kg of L-tryptophan, 0.6g/kg of L-isoleucine, 40mg/kg of vitamin E, 200mg/kg of curcumin, 200mg/kg of thymol essential oil, 100mg/kg of astragalus polysaccharide, 600mg/kg of bacillus subtilis and 300mg/kg of clostridium butyricum, and the carrier of the additive premix is corn cob powder, zeolite powder or bentonite.
And finally adding the additive premix into slow yellow-feather broiler feed according to the proportion of 1 kg/ton.
Example 3:
an additive premix without antibiotics for improving intestinal health of broiler chickens, wherein the dosage of additives L-arginine, L-threonine, L-tryptophan, L-isoleucine, vitamin E, curcumin, tea tree essential oil, chitin and bacillus subtilis is respectively 1.6g/kg of L-arginine, 0.8g/kg of L-threonine, 0.3g/kg of L-tryptophan, 0.5g/kg of L-isoleucine, 30mg/kg of vitamin E, 150mg/kg of curcumin, 300mg/kg of tea tree essential oil, 200mg/kg of chitin, 400mg/kg of bacillus subtilis and 200mg/kg of clostridium butyricum, and the carrier of the additive premix is corncob powder, zeolite powder or bentonite.
Finally, the additive premix is added into the feed of the beard chickens according to the proportion of 1.5 kg/ton.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
In addition, to further illustrate the effect of the additive premix prepared as disclosed in the present application on improving intestinal health of broiler chickens, the inventors conducted the following tests to evaluate:
adding the additive premix prepared above into the feed of Qingyuan partridge chickens, and carrying out the following experiments on the Qingyuan partridge chickens:
1200 Qingyuan partridge hens aged 1 day are selected and randomly divided into 5 groups, each group has 6 repetitions, and 40 chickens are respectively fed to a basic diet (a control group) containing antibiotics, a basic diet +1 kg/ton additive premix (a complete mixing test feed group) without antibiotics for improving the intestinal health of the broiler chickens, a basic diet +1 kg/ton additive premix without antibiotics for improving the intestinal health of the broiler chickens without adding functional amino acids (a functional amino acid reduction test feed group), the basic diet +1 kg/ton additive premix without antibiotics for improving the intestinal health of the broiler chickens does not contain natural plant extracts (natural plant extract reduction test feed group), and the basic diet +1 kg/ton additive premix without antibiotics for improving the intestinal health of the broiler chickens does not contain probiotics (probiotic reduction test feed group), and the test is finished when the feed is fed to 30 days of age.
The additive premix (functional amino acid reduction test material) without antibiotics for improving the intestinal health of yellow-feathered broilers is characterized in that the dosages of the additives such as vitamin E, curcumin, oregano essential oil, mushroom fungus polysaccharide and bacillus subtilis are respectively 20mg/kg of vitamin E, 150mg/kg of curcumin, 150mg/kg of oregano essential oil, 100mg/kg of mushroom fungus polysaccharide, 300mg/kg of bacillus subtilis and 300mg/kg of clostridium butyricum, and the carrier of the additive premix is corncob powder or zeolite powder or bentonite.
An additive premix (natural plant extract reduction test material) without antibiotics for improving the intestinal health of yellow-feathered broilers comprises 1.0g/kg of L-arginine, 0.6g/kg of L-threonine, 0.2g/kg of L-tryptophan, 0.4g/kg of L-isoleucine, 20mg/kg of vitamin E, 150mg/kg of curcumin, 300mg/kg of bacillus subtilis and 300mg/kg of clostridium butyricum, wherein the carrier of the additive premix is corn cob powder or zeolite powder or bentonite.
An additive premix (probiotic reduction test feed) for improving intestinal health of yellow-feathered broilers without antibiotics is disclosed, wherein the dosage of additives L-arginine, L-threonine, L-tryptophan, L-isoleucine, vitamin E, curcumin, oregano essential oil and mushroom fungus polysaccharide is respectively 1.0g/kg of L-arginine, 0.6g/kg of L-threonine, 0.2g/kg of L-tryptophan, 0.4g/kg of L-isoleucine, 20mg/kg of vitamin E, 150mg/kg of curcumin, 150mg/kg of oregano essential oil and 100mg/kg of mushroom fungus polysaccharide, and the carrier of the additive premix is corncob powder or zeolite powder or bentonite.
During the experiment, the test chicken freely takes food and water, other feeding management is carried out according to the conventional feeding management rules, each treatment group adopts corn-soybean meal-corn protein powder type basic feed, and the experimental diet preparation is carried out according to the chicken feeding standard (NY/T-33-2004).
And (4) recording the daily feed intake and the residual feed intake of each column during the test period, and slaughtering and taking jejunum tissues of the tested chickens for index analysis after the test is finished.
Average daily food intake: the weight of feed taken by each chicken per day during the test period.
Daily gain: (30 days old test chicken body weight-1 day old test chicken body weight)/test days.
The material weight ratio is as follows: daily feed intake and daily gain.
When test chickens (30 weeks old) are selected, 2 test chickens close to the average weight are selected repeatedly, and jejunum tissue samples are taken to be tested for the content of Malondialdehyde (MDA); total antioxidant activity (T-AOC), total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px).
Wherein, the analysis of the jejunum antioxidant and immune related genes comprises the following steps: extracting jejunum tissue-like RNA by using a Triol method, and measuring the expression levels of antioxidant genes GPX1, SOD1, HO-1, Nrf2, tight-chain protein genes Claudin-1, Occludin and immune-related genes IL-1 beta, IL-10, TNF-alpha, TLR4 and SIgA by using an RT-PCR method.
Pathological analysis of intestinal tissues: the fixed jejunum tissue sample is embedded in paraffin, and the prepared section is observed under a reverse microscope for pathological changes of the tissue by adopting a conventional hematoxylin-eosin staining method, and is recorded and photographed.
The test results are shown in tables 1 to 4 and FIG. 1.
TABLE 1 Effect of premix additives for improving intestinal health without antibiotics on the growth of Qingyuan partridge chickens
Note: the difference is significant when the same row is marked with a different letter (P < 0.05).
The results in table 1 show that the body weight and daily gain of test chickens at 30 days of age are remarkably increased (P <0.05) compared with the control group by adopting the complete mixed experimental diet; compared with a control group, the weight, the daily gain and the feed weight ratio of 30-day-old test chickens fed with the feed of a functional amino acid reduction group, a natural plant extract reduction group or a probiotic reduction group are not significantly different (P is more than 0.05); the weight ratio of the test chicken feed in the probiotic bacteria-depleted group is not obviously different from that in the control group (P is more than 0.05).
TABLE 2 Effect of additive premix on jejunum antioxidant enzyme genes and activities of Qingyuan partridge chickens
The results in table 2 show that compared with the control group, the mRNA expression quantity, the glutathione peroxidase activity and the total antioxidant capacity (P <0.05) of the test chicken of 30 days old are obviously improved by adopting the completely mixed test feed, and the mRNA expression quantity, the glutathione peroxidase activity and the total antioxidant capacity of the test chicken of jejunum heme oxygenase 1, the nuclear factor E2 related factor 2 and the glutathione peroxidase 1 are improved; there was no significant difference between the superoxide dismutase activity groups in the jejunum (P > 0.05). The mRNA expression quantity, glutathione peroxidase activity and total antioxidant capacity of test chickens 30 days old in the feed of the natural plant extract reduction group are not remarkably different (P is more than 0.05) from those of a control group by adopting the mRNA expression quantity, glutathione peroxidase activity and total antioxidant capacity of test chickens, namely the jejunum heme oxygenase 1, the nuclear factor E2 related factor 2 and the glutathione peroxidase 1 of the test chickens, so that the antioxidant capacity of the intestinal tracts of Qingyuan parthenon chickens is improved by adopting the completely mixed test material.
TABLE 3 influence of additive premix on the relative expression level of mRNA and the content of secretory immunoglobulin A in the jejunum tight junction protein immunocytokine of Qingyuan chicken
The results in table 3 show that compared with the control group, the feeding diet of the complete mixed test group obviously increases the relative expression quantity of the 30-day-old jejunal occludin and mRNA of the secretory immunoglobulin A and the content of the secretory immunoglobulin A, and reduces the relative expression quantity of the mRNA of the Toll-like receptor 4 and the myeloid differentiation factor 88 (P is less than 0.05); the complete mixed experimental group has no significant effect on the relative expression quantity of mRNA of jejunal interleukin-1 beta, interleukin-10 and tumor necrosis factor-alpha (P > 0.05). The relative expression quantity of mRNA and the content of secretory immunoglobulin A of the chicken occludin are obviously reduced by a functional amino acid reduction group (P < 0.05).
TABLE 4 Effect of additive premix on jejunum morphology of Qingyuan chickens
The results in table 4 show that the jejunum villus height and the muscular layer thickness of the Qingyuan partridge chickens of 30 days old are obviously increased (P is less than 0.05) by adopting the complete mixing test feed compared with the control group; while the functional amino acid depletion group tested the chickens had a significant decrease in jejunal villus height and muscle layer thickness (P < 0.05).
In addition, the jejunum hematoxylin-eosin staining microstructure of the Qingyuan far-reaching chickens of 30 days old is 40 times, as shown in fig. 1, and the microscopic result after the jejunum tissue section hematoxylin-eosin staining of fig. 1 also shows that the jejunum villi form of the test chickens in the completely mixed test material group is complete, and the jejunum villi form of the test chickens in the functional amino acid reduction group and the natural plant extract reduction group is damaged to a certain extent compared with the control group.
Therefore, the results are combined to show that the production performance, intestinal oxidation resistance and immunity of the broiler chickens adopting the completely mixed test feed are superior to those of commercial feed groups.
(II) adding the additive premix prepared in the example 2 into the feed of the slow-speed yellow-feathered broilers, and carrying out the following experiments on the slow-speed yellow-feathered broilers:
480 slow yellow-feather broiler cocks of 1 day age are selected and randomly divided into 2 groups, each group is divided into 6 groups, 40 chickens are fed to a basic feed (a control group) containing antibiotics and an additive premix (a test group) which is fed to the basic feed and contains 1 kg/ton of antibiotics and can improve the intestinal health of the broilers respectively, and the test is finished when the chickens are fed to the age of 30 days.
During the experiment, the test chicken freely takes food and water, other feeding management is carried out according to the conventional feeding management rules, each treatment group adopts corn-soybean meal-corn protein powder type basic feed, and the experimental diet preparation is carried out according to the chicken feeding standard (NY/T-33-2004).
And (4) recording the daily feed intake and the residual feed intake of each column during the test period, and slaughtering and taking jejunum tissues of the tested chickens for index analysis after the test is finished.
(III) the additive premix prepared in example 3 was added to the feed of the beard chickens, and the following experiment was performed on the beard chickens:
360 beard chicken hens aged 1 day are selected and randomly divided into 2 groups, each group is divided into 6 groups, 30 chickens are repeated in each group, the chickens are respectively fed with basic feed (a control group) and basic feed plus 1.5 kg/ton additive premix (a test group) without antibiotics for improving the intestinal health of the broiler chickens, and the test is finished when the chickens are fed to 30 days old.
During the experiment, the test chicken freely takes food and water, other feeding management is carried out according to the conventional feeding management rules, each treatment group adopts corn-soybean meal-corn protein powder type basic feed, and the experimental diet preparation is carried out according to the chicken feeding standard (NY/T-33-2004).
And (4) recording the daily feed intake and the residual feed intake of each column during the test period, and slaughtering and taking jejunum tissues of the tested chickens for index analysis after the test is finished.
The product obtained by mixing the above embodiments 2 and 3 can obtain the similar test result to that obtained in embodiment 1 by comparing the same test process with that obtained in embodiment 1, and the details are not repeated herein. And the raw materials of each component in each group of examples can be obtained from the market.
In conclusion, the invention provides the green, safe and environment-friendly feed additive premix without antibiotics for improving the intestinal health of yellow-feathered broilers, so as to relieve the oxidative stress of the intestinal tract and maintain the good growth performance, oxidation resistance and immune function of the yellow-feathered broilers. The experimental results show that the yellow-feathered broiler feed prepared by the invention has the effect of reducing the use of antibiotics, can obviously improve the intestinal health of yellow-feathered broilers, and is suitable for popularization and application in the market.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (7)
1. An additive premix for improving intestinal health of broiler chickens is characterized in that the additive premix consists of an additive and a carrier; wherein the content of the first and second substances,
the additive is composed of the following raw materials in addition: 1.0-2.0 g/kg of arginine, 0.6-0.9 g/kg of threonine, 0.2-0.4 g/kg of tryptophan, 0.4-0.6 g/kg of isoleucine, 0.02-0.04 g/kg of vitamin E, 0.15-0.3 g/kg of curcumin, 0.15-0.3 g/kg of essential oil, 0.1-0.2 g/kg of polysaccharide, 0.3-0.6 g/kg of bacillus subtilis and 0.1-0.3 g/kg of clostridium butyricum;
the carrier is corncob powder, zeolite powder or bentonite; and the addition amount of the carrier is 40-60% of the mass of the additive premix.
2. The additive premix for improving the intestinal health of broiler chickens according to claim 1,
the arginine is preferably L-arginine,
the threonine is preferably L-threonine,
the tryptophan is preferably L-tryptophan,
the isoleucine is preferably L-isoleucine,
the essential oil is preferably oregano essential oil, thymol essential oil, carvacrol essential oil, cinnamaldehyde essential oil or tea tree essential oil,
the polysaccharide is preferably mushroom polysaccharide, astragalus polysaccharide or chitin,
the Bacillus subtilis is preferably a Bacillus subtilis strain,
the clostridium butyricum is preferably clostridium butyricum.
3. Use of the additive premix for improving intestinal health of broiler chickens of claim 1 or 2 in the preparation of broiler feed.
4. The use of the additive premix for improving intestinal health of broiler chickens according to claim 3, wherein said broiler chickens are yellow-feathered broiler chickens, and said broiler chickens are yellow-feathered broiler chickens, qingyuan chickens or beard chickens in the south of the ridge.
5. The application of the additive premix for improving intestinal health of broiler chickens according to claim 3 or 4, wherein the premix is added into 4% premix feed according to the proportion of 25-50 kg/ton.
6. The application of the additive premix for improving the intestinal health of broiler chickens according to claim 3 or 4, wherein the premix is added into a 20% concentrated feed according to the proportion of 5-10 kg/ton.
7. The application of the additive premix for improving the intestinal health of broiler chickens according to claim 3 or 4, wherein the premix is added into a complete formula feed according to the proportion of 1-2 kg/ton.
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