CN113558143A - Feed additive for resisting and promoting growth, preparation method and application thereof - Google Patents
Feed additive for resisting and promoting growth, preparation method and application thereof Download PDFInfo
- Publication number
- CN113558143A CN113558143A CN202110812240.1A CN202110812240A CN113558143A CN 113558143 A CN113558143 A CN 113558143A CN 202110812240 A CN202110812240 A CN 202110812240A CN 113558143 A CN113558143 A CN 113558143A
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- Prior art keywords
- growth
- feed additive
- promoting
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Classifications
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
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Abstract
The invention relates to a substitute-antibiotic growth-promoting feed additive, a preparation method and application thereof. The feed additive for resisting bacteria and promoting growth consists of phytosterol, cholesterol, a composite probiotic agent consisting of bacillus subtilis, bacillus licheniformis and lactobacillus plantarum and a carrier. The feed additive for replacing antibiotics and promoting growth can effectively replace antibiotics, reduce inflammation, inhibit bacterial growth and improve the health level of cultured animals.
Description
[ technical field ] A method for producing a semiconductor device
The invention belongs to the technical field of animal feed. More specifically, the invention relates to a feed additive for resisting and promoting growth, a preparation method of the feed additive for resisting and promoting growth, and application of the feed additive for resisting and promoting growth.
[ background of the invention ]
According to data statistics, about 21 ten thousand tons of antibiotics are produced in China every year, about 18 ten thousand tons of antibiotics are used in China, and the quantity of the antibiotics used in livestock husbandry and feed industries is up to 9.7 ten thousand tons and accounts for about 54 percent. Since the early 50 s of the last century, antibiotics are used as feed additives in the animal husbandry industry, and for a long time, people and animals share antibacterial drugs, so that the drug resistance of animal-derived bacteria is increased, and the health of the people is threatened. Under the background, in 3 months in 2019, 194 th bulletin title issued by agricultural rural departments is deployed according to a national suppressed bacteria drug-resistant action plan (2016 + 2020) and a national suppressed animal source bacteria drug-resistant action plan (2017 + 2020), so that the safety of animal-derived food and public health in China is maintained, and the implementation of a drug feed additive quit plan is determined. 194, it is pointed out that all varieties of growth-promoting drug feed additives except traditional Chinese medicines are withdrawn from the 2020 on 1/1; from 7/1/2020, feed manufacturers stop producing commercial feeds containing growth-promoting drug feed additives (except traditional Chinese medicines).
At present, the substitute antibiotics and the growth-promoting feed additive are mainly traditional Chinese medicine feed additives, but the traditional Chinese medicine medicament has a complex formula and a higher price, the traditional Chinese medicine active substances are generally easy to be oxidized, deliquesced and inactivated, the action effect is unstable, and the breeding cost is increased, so that an efficient and stable substitute antibiotic and growth-promoting feed additive applied to the breeding industry is urgently needed.
Phytosterols were first found to exhibit excellent effects in cholesterol resistance and prevention of cardiovascular diseases, and this function was manifested in increasing slaughter and lean meat percentage in economic animals. In addition, it has been found in several studies that it also has an anti-inflammatory effect (Michael Valerio et al, phytosterides ameliorant clinical manifestations and infection in experimental animals of phytosterine encephalyitis [ J ]. Influmation Research,2011,60(5) ], bacteriostatic effect (Cao Li stand, Plumbum rubrum, Korean, Tian Zi Jing. extraction of Phytosterols from oil of Araneus and bacteriostatic effect thereof [ J ]. Natural products Research and development, 2010,22(02):334 338), anti-tumor effect (Gemma therapeutic), Journal carretes Ecol-Gil, Enrique Lerma, Journal Julve, Cristina Pons, Anna Cabr, Montsin Cocol, Emblem coli G-Gi, Enrique Nu, Stem J. J.12, Reishi, Biosterol J.3, Biocide J.J.: biological acids J.12. and Biocide J.3. origin J.12. origin J. Adrio. biological acids, protein J. Discovery Today,2002,7(14):775-778) and the effect of prolonging the growth life of animals. After 2013, the phytosterol is formally added into the catalogue of feed additive varieties (2013). Although the anti-inflammatory and bacteriostatic effects of phytosterol added alone are obvious, the effect is equivalent to that of polymyxin E (Qiabing Hu, ZHao Zhu, ShenglinFang, Yiwei Zhang, Jie Feng. phytosterides animal activity and extract anti-inflammatory activity in peptides [ J ]. Journal of the Science of Food and Agriculture,2017,97(12)), but the growth promoting effect is unstable in different experiments and is not widely applied.
The inventor aims at the problems of high cost, unstable effect, poor growth promoting effect of the formula of the phytosterol additive and the like of the traditional Chinese medicine, and finally completes the invention through a large number of experimental researches and analytical summarization on the basis of summarizing the prior art.
[ summary of the invention ]
[ problem to be solved ]
The invention aims to provide an anti-growth promoting feed additive.
The invention also aims to provide a preparation method of the substitute antibody growth-promoting feed additive.
Another object of the present invention is to provide the use of the anti-growth promoting feed additive.
[ solution ]
The invention is realized by the following technical scheme.
The invention relates to a feed additive for replacing antibiotics and promoting growth.
The composition of the substitute anti-growth-promoting feed additive is as follows: in parts by weight
According to a preferred embodiment of the present invention, the composition of the alternative anti-growth promoting feed additive is as follows: in parts by weight
According to another preferred embodiment of the present invention, the phytosterol is one or more phytosterols selected from the group consisting of beta-sitosterol, stigmasterol, campesterol and brassicasterol.
According to another preferred embodiment of the invention, the complex probiotic is composed of bacillus subtilis preparation, bacillus licheniformis preparation and lactobacillus plantarum preparation.
According to another preferred embodiment of the invention, the Bacillus subtilis is Bacillus subtilis CCTCC No.14929, CGMCC No.13714 or CGMCC No. 2434; the bacillus licheniformis is bacillus licheniformis CGMCC No.6510, CGMCC No.18048 or CGMCC No. 21267; the lactobacillus plantarum is lactobacillus plantarum CGMCC NO.12934, CGMCC NO.12554 or CGMCC NO. 16871.
According to another preferred embodiment of the invention, the viable bacteria count of the bacillus subtilis preparation, the bacillus licheniformis preparation and the lactobacillus plantarum preparation is more than 50 hundred million/g.
According to another preferred embodiment of the present invention, the carrier is one or more selected from the group consisting of silica, zeolite powder, rice hull powder, maltodextrin and starch, and has a particle size of 40 to 80 mesh.
The invention also relates to a preparation method of the anti-growth promoting feed additive. The preparation method comprises the following preparation steps:
A. preparation of composite probiotic
Mixing a bacillus subtilis preparation, a bacillus licheniformis preparation and a lactobacillus plantarum preparation to prepare a composite probiotic preparation;
B. pulverizing
Respectively crushing 20-50 parts by weight of phytosterol, 0.1-10.0 parts by weight of cholesterol, 10-30 parts by weight of the composite probiotic prepared in the step A and 10-70 parts by weight of carrier to obtain particles with the particle size of 40-80 meshes; the phytosterol is one or more phytosterols selected from beta-sitosterol, stigmasterol, campesterol or brassicasterol; the carrier is one or more carriers selected from silicon dioxide, zeolite powder, rice hull powder, maltodextrin or starch;
C. mixing
And D, pouring the phytosterol powder, the cholesterol powder, the composite probiotic agent powder and the carrier powder obtained in the step B into a mixer, and stirring and mixing for more than 10min at the stirring speed of 30-60 rpm to obtain the phytosterol anti-growth promoting feed additive which is capable of resisting and promoting growth and has the viable count of more than 50 hundred million/g of bacillus subtilis agent, bacillus licheniformis agent and lactobacillus plantarum agent.
The invention also relates to the application of the anti-growth-promoting feed additive or the anti-growth-promoting feed additive prepared by the preparation method, wherein the addition amount of the anti-growth-promoting feed additive in the feed of the fed animals is 0.2-2.0% by weight.
According to another preferred embodiment of the invention, the farm animal is a livestock farm animal, a poultry farm animal or an aquaculture animal.
The present invention will be described in more detail below.
The invention relates to a feed additive for replacing antibiotics and promoting growth.
The composition of the substitute anti-growth-promoting feed additive is as follows: in parts by weight
The basic effect of the phytosterol in the anti-growth promoting feed additive can inhibit the release of some proinflammatory factors and chemotactic factors, inhibit STAT1 signal channels, effectively reduce the infiltration of cell inflammation, inhibit the migration of transcription factors (NF-kB), enhance the cytotoxicity capability of Natural Killer (NK) cells, inhibit the generation of carcinogens and the growth, invasion and metastasis of cancer cells, effectively reduce the possibility of animal inflammation and canceration, and prolong the life of animals. The phytosterol can also effectively inhibit the growth of harmful strains such as staphylococcus aureus, salmonella, enterococcus faecium and the like, can not excessively inhibit the growth of strains such as bacillus subtilis, bacillus licheniformis, lactobacillus plantarum and the like in the composite probiotics, promotes the intestinal function, reduces the diarrhea rate and the death rate, and is an animal anti-bacterial feed formula with anti-inflammatory and bacteriostatic functions.
The phytosterol is one or more phytosterols selected from beta-sitosterol, stigmasterol, campesterol and brassicasterol.
The phytosterols used in the present invention are all commercially available products, such as those sold by Shaanxi Haas Biotechnology Ltd.
The cholesterol has the basic effect of promoting cell growth by cooperating with the phytosterol in the anti-growth promoting feed additive, so that the phytosterol can be better combined with growth hormone and ribonucleoprotein to form a growth hormone-phytosterol-ribonucleoprotein complex, the half life of the growth hormone is prolonged, and the feed conversion rate and slaughter economic index of cultured animals at each stage are obviously improved.
The cholesterol used in the present invention is a currently marketed product, such as cholesterol sold by Angel chemical technology, Inc., Anhui.
In the present invention, the complex probiotic is understood to be a complex probiotic consisting of a bacillus subtilis agent, a bacillus licheniformis agent and a lactobacillus plantarum agent.
The composite probiotics in the alternative anti-growth-promoting feed additive has the main effects of enhancing the palatability of the feed additive, having a certain food calling effect and improving the feed intake and daily gain.
The composite probiotic agent used in the invention is composed of a bacillus subtilis agent, a bacillus licheniformis agent and a lactobacillus plantarum agent, wherein the bacillus subtilis is bacillus subtilis CCTCC NO.14929, CGMCC No.13714 or CGMCC No. 2434; the bacillus licheniformis is bacillus licheniformis CGMCC No.6510, CGMCC No.18048 or CGMCC No. 21267; the lactobacillus plantarum is lactobacillus plantarum CGMCC NO.12934, CGMCC NO.12554 or CGMCC NO. 16871.
The bacillus subtilis agent, the bacillus licheniformis agent and the lactobacillus plantarum agent used in the invention are all products sold in the current market. For example, Bacillus subtilis agent sold by Luoyang Ogaku Bye Biotech Co., Ltd, Bacillus licheniformis agent sold by Jinnan Tiantianxiang Co., Ltd, and Lactobacillus plantarum agent sold by Shenyang Huaxing Biotech Co., Ltd.
Of course, other strains of Bacillus subtilis, Bacillus licheniformis, and Lactobacillus plantarum may be used herein, provided that they do not adversely affect the performance and utility of the anti-growth-promoting feed additive of the present invention, and are within the scope of the present invention.
According to the invention, in the alternative anti-growth-promoting feed additive, the viable count of the bacillus subtilis preparation, the bacillus licheniformis preparation and the lactobacillus plantarum preparation is more than 50 hundred million/gram. It is not preferable if the number of viable bacteria per microbial inoculum is less than 50 hundred million/gram because less than 50 hundred million/gram does not exert the palatability-enhancing and food calling effects.
The viable count of the bacillus subtilis preparation is detected according to a GB/T26428-.
The viable count of the bacillus licheniformis agent is detected according to the NYT1461-2007 standard detection method.
The viable count of the lactobacillus plantarum is detected according to a DB37T3405-2018 standard detection method.
In the present invention, a carrier is understood to be a substance carrying an active ingredient.
The main function of the carrier in the anti-growth promoting feed additive is to facilitate the dispersion effect of the phytosterol, cholesterol and composite probiotics in the feed.
The carrier used in the present invention is one or more carriers selected from the group consisting of silica, zeolite powder, powdered rice hull, maltodextrin, and starch.
The maltodextrin is a product sold in the market at present, such as a product sold by Shandong Nuanzai Biotech limited company.
The starch used in the present invention is, for example, resistant starch, amylopectin, amylose or alpha-starch, which are all products currently marketed, for example alpha-starch sold by Weifang telebentonite Ltd.
The silica, zeolite powder and rice hull powder carriers used in the invention are all products sold in the market at present, such as silica sold by Hefu Sheng biological products Co.
The particle size of the particles is 40-80 meshes. It is not feasible that the particle size of these carriers exceeds the stated range, since this particle size makes it possible to achieve a higher degree of mixing homogeneity, ensures homogeneity of the nutrient substance and improves the absorption rate. Too small a particle size may affect palatability, increase swallowing difficulty, even cause gastric ulcer in the fed animals, and increase power consumption and motor wear in feed processing, increasing cost.
In the present invention, when the contents of cholesterol, the complex probiotic and the carrier are within the above range, if the content of phytosterol is less than 20 parts by weight, the anti-inflammatory and growth promoting effects are insignificant; if the content of the phytosterol is more than 50 parts by weight, absorption inhibition exists when the phytosterol is ingested at a high rate, and the absorption rate cannot be improved; therefore, the content of the phytosterol is reasonable to be 20-50 parts by weight, and preferably 30-40 parts by weight;
similarly, when the contents of the phytosterol, the composite probiotic and the carrier are within the range, if the content of the cholesterol is less than 0.1 part by weight, the synergistic effect of the phytosterol cannot be achieved; if the content of cholesterol is more than 10.0 parts by weight, the cost is increased, and the high-cholesterol feed increases the risk of diseases for raising animals; therefore, the content of cholesterol is suitably 0.1 to 10.0 parts by weight, preferably 1.0 to 8.0 parts by weight;
when the contents of the phytosterol, the cholesterol and the carrier are in the range, if the content of the composite probiotic is lower than 10 parts by weight, the effect is not obvious; if the content of the composite probiotic is higher than 30 parts by weight, the composite bacteria consume free oxygen in the internal environment, so that oxygen deficiency of the intestinal tract is caused, the growth of beneficial anaerobic bacteria is promoted, organic acids such as lactic acid and the like are generated, the pH value of the intestinal tract is reduced, and the risk of diseases is increased; therefore, the content of the composite probiotic is proper to be 10 to 30 parts by weight, and preferably 16 to 24 parts by weight;
when the contents of the phytosterol, the cholesterol and the composite probiotic are in the range, if the content of the carrier is less than 10 parts by weight, the phytosterol, the cholesterol and the composite probiotic cannot be uniformly mixed; if the content of the carrier is more than 70 parts by weight, the effective substances are too low, and the effect is reduced; therefore, the content of the carrier is suitably 10 to 70 parts by weight, preferably 25 to 55 parts by weight;
preferably, the composition of the anti-growth promoting feed additive is as follows: in parts by weight
The invention also relates to a preparation method of the anti-growth promoting feed additive. The preparation method comprises the following preparation steps:
A. preparation of composite probiotic
Mixing a bacillus subtilis preparation, a bacillus licheniformis preparation and a lactobacillus plantarum preparation to prepare a composite probiotic preparation;
B. pulverizing
Respectively crushing 20-50 parts by weight of phytosterol, 0.1-10.0 parts by weight of cholesterol, 10-30 parts by weight of the composite probiotic prepared in the step A and 10-70 parts by weight of carrier to obtain particles with the particle size of 40-80 meshes; the phytosterol is one or more phytosterols selected from beta-sitosterol, stigmasterol, campesterol or brassicasterol; the carrier is one or more carriers selected from silicon dioxide, zeolite powder, rice hull powder, maltodextrin or starch;
C. mixing
And D, pouring the phytosterol powder, the cholesterol powder, the composite probiotic powder and the carrier powder obtained in the step B into a mixer, and stirring and mixing for more than 10min at the stirring speed of 30-60 rpm to obtain the phytosterol anti-growth-promoting feed additive which is capable of replacing the anti-growth of the lactobacillus plantarum and has the viable count of more than 50 hundred million/g.
The conditions of phytosterol, cholesterol, complex probiotics and carrier have been described above, and thus are not described herein again.
The crushing equipment and the stirring equipment used in the invention are all products which are commonly used in the technical field and sold in the market at present.
The invention also relates to the application of the anti-growth-promoting feed additive or the anti-growth-promoting feed additive prepared by the preparation method. On the one hand, the feed additive can effectively improve the immunity and health level of animals through the anti-inflammatory and bacteriostatic effects of the phytosterol, on the other hand, when the cholesterol is added, the phytosterol can promote the cell growth, and can be better combined with growth hormone and ribonucleoprotein to form a growth hormone-phytosterol-ribonucleoprotein complex, so that the half-life period of the growth hormone is prolonged, and the feed conversion rate and slaughter economic indexes of various stages of cultured animals are obviously improved.
The addition amount of the substitute antibiotic growth-promoting feed additive in the feed for the raised animals is 0.2-2.0% by weight. If the addition amount of the substitute antibiotic growth-promoting feed additive is less than 0.2 percent, the use effect is reduced; if the addition amount of the substitute anti-growth-promoting feed additive is higher than 2.0%, absorption inhibition exists when the feed is ingested at a high rate, the absorption rate cannot be improved, and the disease risk is increased; therefore, the addition amount of the substitute antibiotic growth promoting feed additive is preferably 0.2 to 2.0%.
According to the invention, the farm animal is a livestock farm animal, a poultry farm animal or an aquaculture animal.
The livestock breeding animals are, for example, pigs, cattle or sheep.
The poultry breeding animals are, for example, chickens, ducks or geese.
The aquatic livestock may be fish, shrimp, shellfish or crab.
[ advantageous effects ]
The invention has the beneficial effects that:
1. the feed additive for replacing antibiotics and promoting growth can effectively replace antibiotics, reduce inflammation, inhibit bacterial growth and improve the health level of cultured animals;
2. the invention combines the phytosterol and the cholesterol, so that the cholesterol activates the cell growth, and the effect of promoting the growth of the phytosterol is enhanced.
[ detailed description ] embodiments
The invention will be better understood from the following examples.
Example 1: the invention relates to a feed additive for replacing antibiotics and promoting growth
The implementation of this example is as follows:
the anti-displacement growth-promoting feed additive consists of 40 parts by weight of beta-sitosterol phytosterol, 2.0 parts by weight of cholesterol, 10 parts by weight of a composite probiotic microbial agent consisting of bacillus subtilis CCTCC No.14929 microbial agent, bacillus licheniformis CGMCC No.6510 microbial agent and lactobacillus plantarum CGMCC No.12934 microbial agent according to the weight ratio of 1:1:1 and 70 parts by weight of silicon dioxide carrier.
The substitute antibiotic growth-promoting feed additive is prepared according to the following preparation steps:
A. preparation of composite probiotics
Mixing a bacillus subtilis CCTCC No.14929 microbial inoculum, a bacillus licheniformis CGMCC No.6510 microbial inoculum and a lactobacillus plantarum CGMCC No.12934 microbial inoculum according to the weight ratio of 1:1:1 to prepare a composite probiotic agent;
B. pulverizing
Respectively crushing 40 parts by weight of beta-sitosterol phytosterol, 2.0 parts by weight of cholesterol, 10 parts by weight of the composite probiotics obtained in the step A and 70 parts by weight of silicon dioxide carrier to 40 meshes of particle size;
C. mixing
And D, pouring the beta-sitosterol phytosterol powder, the cholesterol powder, the composite probiotic powder and the silicon dioxide carrier powder obtained in the step B into a mixer, and stirring and mixing for 15min under the condition of stirring speed of 30-60 rpm to obtain the phytosterol substitute anti growth promotion feed additive with substitute anti growth promotion function, wherein the viable count of bacillus subtilis, bacillus licheniformis and lactobacillus plantarum is more than 50 hundred million/g.
Animal feeding was carried out according to the experimental procedure specified in GBZ 31813-2015.
Experimental animals: selecting 180 weaned piglets of 23 days old according to the principle of similar body weight and similar genetic basis, wherein 90 piglets are selected as boars and 90 piglets are selected as sows;
the experimental method comprises the following steps: weaned piglets were randomly divided into three groups: a blank control group, a drug control group and an experimental group, wherein each group is provided with 3 repeats, each 20 repeats is a column, and the number of boars and sows in each column is the same; the experimental period was 21 days. Wherein:
blank control group: only piglet feed was used;
drug control group: 100mg/kg polymyxin E + piglet feed;
experimental groups: 2% feed additive + piglet feed was prepared in this example.
The piglet feed is sold as piglet compound feed 851 by twins (group) GmbH
These weaned piglets were fed with a piglet feed sold as a suckling pig compound feed 851 by twins (group) GmbH
The experimental results are as follows: the experimental data and the efficacy evaluation index are shown in table 1.
Table 1: feeding effect of weaned piglets
The experimental results listed in table 1 show that compared with the blank control, the effect of the experimental group and the drug control group is similar, so that the diarrhea rate of piglets can be reduced remarkably; compared with a blank control group and a drug control group, the experimental group can effectively improve the growth performance of piglets and feed intake, and the phytosterol feed additive is more efficient and safer, can effectively improve the disease resistance of piglets and promotes the growth of piglets.
Example 2: the invention relates to a feed additive for replacing antibiotics and promoting growth
The implementation of this example is as follows:
the feed additive consists of 20 parts by weight of brassicasterol phytosterol, 0.1 part by weight of cholesterol, 16 parts by weight of composite probiotic preparation consisting of bacillus subtilis CGMCC No.13714, bacillus licheniformis CGMCC No.18048 and lactobacillus plantarum CGMCC No.12554 according to the weight ratio of 2:2:1 and 10 parts by weight of maltodextrin carrier.
The substitute antibiotic growth promoting feed additive is prepared according to the following preparation method:
A. preparation of composite probiotics
Mixing bacillus subtilis CGMCC No.13714 microbial inoculum, bacillus licheniformis CGMCC No.18048 microbial inoculum and lactobacillus plantarum CGMCC No.12554 microbial inoculum according to the weight ratio of 2:2:1 to prepare composite probiotics;
B. pulverizing
Respectively crushing 20 parts by weight of brassicasterol phytosterol, 0.1 part by weight of cholesterol, 16 parts by weight of the composite probiotic agent obtained in the step A and 10 parts by weight of maltodextrin carrier to the granularity of 80 meshes;
C. mixing
And D, pouring the rapeseed sterol phytosterol powder, the cholesterol powder, the composite probiotic powder and the maltodextrin carrier powder obtained in the step B into a mixer, and stirring and mixing for 13min at the stirring speed of 30-60 rpm to obtain the phytosterol substitute anti growth promotion feed additive with substitute anti growth promotion function, wherein the viable count of bacillus subtilis, bacillus licheniformis and lactobacillus plantarum is more than 50 hundred million/g.
According to the principle of similar body weight and genetic basic similarity, 180 white feather broilers (half of each cock and half of each hen) of 1 day old are selected and randomly divided into three groups: blank control group, drug control group and experimental group, each group has 3 replicates, each replicate 20 is a column, each column is half of cock and hen; the chickens were fed with the special daily ration for chicks, and the test period was 21 days. The experimental data and the efficacy evaluation index are shown in table 2.
Table 2: feeding effect of white feather broiler
The experimental results show that compared with a blank control, the experimental group and the drug group can effectively reduce the diarrhea rate of the chicks, obviously reduce the severity of diarrhea and obviously reduce the feeding mortality. Meanwhile, compared with a blank control group, the phytosterol formula group can obviously improve the growth performance of the chicks. The phytosterol feed additive is more efficient and safer, can effectively improve the disease resistance of the chicks and promote the growth of the chicks.
Example 3: the invention relates to a feed additive for replacing antibiotics and promoting growth
The implementation of this example is as follows:
the anti-displacement growth-promoting feed additive is composed of 30 parts by weight of stigmasterol phytosterol, 6.0 parts by weight of cholesterol, 30 parts by weight of composite probiotics and 30 parts by weight of zeolite powder carrier, wherein the composite probiotics are composed of bacillus subtilis CGMCC No.2434 microbial inoculum, bacillus licheniformis CGMCC No.21267 microbial inoculum and lactobacillus plantarum CGMCC No.16871 microbial inoculum according to the weight ratio of 3:2: 2.
The substitute antibiotic growth promoting feed additive is prepared according to the following preparation method:
A. preparation of composite probiotics
Mixing a bacillus subtilis CGMCC No.2434 microbial inoculum, a bacillus licheniformis CGMCC No.21267 microbial inoculum and a lactobacillus plantarum CGMCC No.16871 microbial inoculum according to the weight ratio of 3:2:2 to prepare a composite probiotic agent;
B. pulverizing
Respectively crushing 30 parts by weight of stigmasterol plant sterol, 6.0 parts by weight of cholesterol, 30 parts by weight of the composite probiotics obtained in the step A and 30 parts by weight of the zeolite powder carrier to obtain the granularity of 60 meshes;
C. mixing
And D, pouring the stigmasterol plant sterol powder, the cholesterol powder, the composite probiotic powder and the zeolite powder carrier powder obtained in the step B into a mixer, and stirring and mixing for 11min under the condition of stirring speed of 30-60 rpm to obtain the plant sterol substitute anti growth promotion feed additive which is used for substituting anti growth promotion and has the viable count of more than 50 hundred million/g of bacillus subtilis, bacillus licheniformis and lactobacillus plantarum.
Animal feeding experiments were performed according to the same experimental method as described in example 1, except that 50 sows of 6 months of age were selected, 10 sows per group, for an experimental period of 1 month, according to the principles of similar body weight and similar genetic basis. The experimental data and the efficacy evaluation index are shown in Table 3.
Table 3: feeding effect of 6-month-old sows
The experimental results show that compared with a blank control group and a drug control group, the experimental group can effectively improve the growth performance of the sows, improve the feed intake and daily gain, and is safe and effective.
Example 4: the invention relates to a feed additive for replacing antibiotics and promoting growth
The implementation of this example is as follows:
the feed additive consists of 50 parts by weight of campesterol phytosterol, 10.0 parts by weight of cholesterol, 24 parts by weight of composite probiotic preparation consisting of bacillus subtilis CCTCC No.14929 microbial inoculum, bacillus licheniformis CGMCC No.21267 microbial inoculum and lactobacillus plantarum CGMCC No.12554 microbial inoculum according to the weight ratio of 1:3:3 and 50 parts by weight of starch carrier.
The substitute antibiotic growth promoting feed additive is prepared according to the following preparation method:
A. preparation of composite probiotics
Mixing bacillus subtilis CCTCC No.14929 microbial inoculum, bacillus licheniformis CGMCC No.21267 microbial inoculum and lactobacillus plantarum CGMCC No.12554 microbial inoculum according to the weight ratio of 1:3:3 to prepare composite probiotics;
B. pulverizing
Respectively crushing 50 parts by weight of campesterol phytosterol, 10.0 parts by weight of cholesterol, 24 parts by weight of the composite probiotic agent obtained in the step A and 50 parts by weight of the starch carrier to 50 meshes;
C. mixing
And D, pouring the campesterol phytosterol powder, the cholesterol powder, the composite probiotic powder and the starch carrier powder obtained in the step B into a mixer, and stirring and mixing for 15min under the condition of a stirring speed of 30-60 rpm to obtain the phytosterol anti-growth promoting feed additive which has the anti-growth promoting effect and the viable count of more than 50 hundred million/g of bacillus subtilis, bacillus licheniformis and lactobacillus plantarum.
Animal feeding experiments were performed according to the experimental procedure described in example 2, except that Qingyuan chickens were used as experimental animals. The experimental data and the efficacy evaluation index are shown in Table 4.
Table 4: feeding effect of Ma chicken
The experimental result shows that compared with a blank control group and a medicine group, the experimental group can effectively reduce the diarrhea rate of the partridge, can obviously reduce the severity of diarrhea and obviously reduce the feeding mortality. Meanwhile, compared with a blank control group, the phytosterol formula group can obviously improve the growth performance of the partridge chickens. The phytosterol feed additive is more efficient and safer, can effectively improve the disease resistance of the partridge chickens and promote the growth of the partridge chickens.
Claims (10)
1. A feed additive for replacing antibiotics and promoting growth is characterized by comprising the following components: in parts by weight
2. A feed additive for resisting and promoting growth according to claim 1, characterized in that it consists of: in parts by weight
3. The resistance-promoting growth feed additive according to claim 1 or 2, wherein the phytosterol is one or more phytosterols selected from the group consisting of β -sitosterol, stigmasterol, campesterol and brassicasterol.
4. The feed additive for resisting and promoting growth according to claim 1 or 2, wherein the complex probiotic is composed of bacillus subtilis agent, bacillus licheniformis agent and lactobacillus plantarum agent.
5. The feed additive of claim 4 wherein said Bacillus subtilis is Bacillus subtilis CCTCC No.14929, CGMCC No.13714 or CGMCC No. 2434; the bacillus licheniformis is bacillus licheniformis CGMCC No.6510, CGMCC No.18048 or CGMCC No. 21267; the lactobacillus plantarum is lactobacillus plantarum CGMCC NO.12934, CGMCC NO.12554 or CGMCC NO. 16871.
6. The feed additive for replacing antibiotics and promoting growth according to claim 4, wherein the viable count of the bacillus subtilis agent, the bacillus licheniformis agent and the lactobacillus plantarum agent is more than 50 hundred million/gram.
7. The feed additive of substituting anti-growth promotion according to claim 1 or 2, characterized in that the carrier is one or more carriers selected from silica, zeolite powder, rice hull powder, maltodextrin or starch, and their particle size is 40-80 mesh.
8. The method for preparing the anti-growth-promoting feed additive according to any one of claims 1 to 7, wherein the preparation method comprises the following steps:
A. preparation of composite probiotics
Mixing a bacillus subtilis preparation, a bacillus licheniformis preparation and a lactobacillus plantarum preparation to prepare a composite probiotic preparation;
B. pulverizing
Respectively crushing 20-50 parts by weight of phytosterol, 0.1-10.0 parts by weight of cholesterol, 10-30 parts by weight of the composite probiotic prepared in the step A and 10-70 parts by weight of carrier to obtain particles with the particle size of 40-80 meshes; the phytosterol is one or more phytosterols selected from beta-sitosterol, stigmasterol, campesterol or brassicasterol; the carrier is one or more carriers selected from silicon dioxide, zeolite powder, rice hull powder, maltodextrin or starch;
C. mixing
And D, pouring the phytosterol powder, the cholesterol powder, the composite probiotic powder and the carrier powder obtained in the step B into a mixer, and stirring and mixing for more than 10min at the stirring speed of 30-60 rpm to obtain the phytosterol anti-growth-promoting feed additive which is capable of replacing the anti-growth of the lactobacillus plantarum and has the viable count of more than 50 hundred million/g.
9. Use of the anti-growth-promoting feed additive according to any one of claims 1 to 7 or the anti-growth-promoting feed additive prepared by the preparation method according to claim 8, wherein the anti-growth-promoting feed additive is added to the feed of the farm animals in an amount of 0.2 to 2.0% by weight.
10. Use according to claim 9, characterized in that the farm animal is a livestock farm animal, a poultry farm animal or an aquaculture animal.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112877257A (en) * | 2021-03-18 | 2021-06-01 | 昆明理工大学 | Bacillus licheniformis and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104664173A (en) * | 2015-03-20 | 2015-06-03 | 江门海大饲料有限公司 | Efficient environment-friendly antibiotic-free compound feed for piglets |
| CN109997960A (en) * | 2019-05-06 | 2019-07-12 | 北京绿摇饲料科技有限公司 | A kind of weanling pig Transition Materials and its application method |
-
2021
- 2021-07-19 CN CN202110812240.1A patent/CN113558143A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104664173A (en) * | 2015-03-20 | 2015-06-03 | 江门海大饲料有限公司 | Efficient environment-friendly antibiotic-free compound feed for piglets |
| CN109997960A (en) * | 2019-05-06 | 2019-07-12 | 北京绿摇饲料科技有限公司 | A kind of weanling pig Transition Materials and its application method |
Non-Patent Citations (1)
| Title |
|---|
| HU Q , ZHAO Z , FANG S , ET AL.: "Phytosterols improve immunity and exert anti‐inflammatory activity in weaned piglets", 《JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112877257A (en) * | 2021-03-18 | 2021-06-01 | 昆明理工大学 | Bacillus licheniformis and application thereof |
| CN112877257B (en) * | 2021-03-18 | 2023-04-07 | 昆明理工大学 | Bacillus licheniformis and application thereof |
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