CN112219946B - Composite microecological preparation for epidemic prevention of suckling pigs - Google Patents

Composite microecological preparation for epidemic prevention of suckling pigs Download PDF

Info

Publication number
CN112219946B
CN112219946B CN202011023246.2A CN202011023246A CN112219946B CN 112219946 B CN112219946 B CN 112219946B CN 202011023246 A CN202011023246 A CN 202011023246A CN 112219946 B CN112219946 B CN 112219946B
Authority
CN
China
Prior art keywords
percent
vitamin
composite
mixture
mixing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202011023246.2A
Other languages
Chinese (zh)
Other versions
CN112219946A (en
Inventor
余忠丽
恽辉
赵大伟
王俊青
劳泰财
崔志英
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xinjiang Xipu Biological Science & Technology Co ltd
Original Assignee
Xinjiang Xipu Biological Science & Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xinjiang Xipu Biological Science & Technology Co ltd filed Critical Xinjiang Xipu Biological Science & Technology Co ltd
Priority to CN202011023246.2A priority Critical patent/CN112219946B/en
Publication of CN112219946A publication Critical patent/CN112219946A/en
Application granted granted Critical
Publication of CN112219946B publication Critical patent/CN112219946B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Birds (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Fodder In General (AREA)

Abstract

The invention belongs to the field of composite microecologics in the field of breeding, and particularly relates to a composite microecologics for epidemic prevention of porkets. The composite microecological preparation provided by the invention comprises the following components in percentage by weight: 0.8 to 1.2 percent of ornithine, 0.5 to 1.0 percent of lysine, 0.3 to 0.5 percent of arginine, 1.2 to 1.5 percent of chitosan oligosaccharide, 0.3 to 0.6 percent of garlicin, 1 to 1.5 percent of compound vitamin, 0.3 to 0.5 percent of digestion promoter, 1.5 to 2 percent of compound microorganism strain, 0.5 to 0.8 percent of corncob meal and 90.4 to 93.6 percent of purified water. The composite microecological preparation provided by the invention can effectively improve the resistance of the suckling pigs to exogenous pathogenic bacteria, reduce the incidence of the exogenous pathogenic bacteria and contribute to the healthy growth of the suckling pigs while providing nutrient substances for the suckling pigs.

Description

Composite microecological preparation for epidemic prevention of suckling pigs
Technical Field
The invention belongs to the field of composite microecologics in the field of cultivation, and particularly relates to a composite microecologics for epidemic prevention of suckling pigs.
Background
Suckling pigs refer to piglets which have not yet been completely weaned, generally pigs born less than two months. In the process of weaning the suckling pigs, due to the fact that the suckling pigs are often influenced by factors such as hunger and stress, overfeeding is very easy to cause diarrhea, and therefore feeding is seldom carried out, on one hand, freshness of feed can be guaranteed, appetite can be increased, the appetite of the suckling pigs can be effectively stimulated, feed intake can be increased, the digestion capacity of the suckling pigs can be exercised as soon as possible, and the fastest growth speed is guaranteed. Meanwhile, clean drinking water is provided, and the trough is guaranteed to be clean. The feeding method of free feeding is adopted immediately after weaning, but the method is not beneficial to improving the feed intake of the suckling pigs.
For selecting the piglet feed, on one hand, the piglet feed needs to be considered from the surface, such as easy digestion, no diarrhea and large feed intake; on the other hand, the growth performance needs to be evaluated, and for the suckling pigs, the most important is the growth speed of the suckling pigs, the suckling pigs grow fast, and the marketing time of the whole fattening period is shortened. Therefore, the choice of piglet feed is very important for the growth of the piglets. The existing commercial suckling pig feed can ensure the growth speed of suckling pigs to a certain extent, but cannot provide enough nutrient substances for the suckling pigs, so that the suckling pigs have poor resistance, are easily interfered by diseases, and sometimes cannot even survive.
Based on this, chinese patent CN103444784B discloses a compound microecological preparation for adjuvant epidemic prevention of live pigs, which can block the transmission of pathogenic bacteria in ecological pig farms, thereby improving the disease resistance of live pig breeding, but the preparation can not block the pathogenic bacteria infection well, only reduces the transmission process, and the preparation is generally suitable for pigs after weaning completely, while the sucking pigs need to be more careful in the feed selection process due to poor self-resistance.
Meanwhile, the chinese patent application CN105285399A discloses a liquid composite microecological preparation for piglets and a preparation method thereof, wherein the composite microecological preparation is developed by strengthening the stability of the microecological preparation, especially the stability of lactic acid bacteria, by chitosan oligosaccharide aiming at the characteristics of piglet intestinal flora development and piglet feeding management, and can effectively maintain the balance of strains in vivo, but the composite microecological preparation cannot resist the infection of foreign bacteria and viruses, is easy to cause various diseases, and causes harm to the body of pigs.
In conclusion, the prior art generally lacks a microecological preparation which can be used for epidemic prevention of the suckling pigs, so that the resistance of the suckling pigs to foreign germs is improved, and the occurrence of diseases is reduced.
Disclosure of Invention
Aiming at the defects generally existing in the prior art, the invention provides a composite microecological preparation for preventing piglets from epidemic. The composite microecological preparation provided by the invention can effectively improve the resistance of the suckling pigs to exogenous pathogenic bacteria, reduce the incidence of the exogenous pathogenic bacteria and contribute to the healthy growth of the suckling pigs while providing nutrient substances for the suckling pigs.
In order to achieve the purpose, the invention adopts the technical scheme that:
a composite microecological preparation comprises the following components in percentage by weight: 0.8 to 1.2 percent of ornithine, 0.5 to 1.0 percent of lysine, 0.3 to 0.5 percent of arginine, 1.2 to 1.5 percent of chitosan oligosaccharide, 0.08 to 0.15 percent of garlicin, 1 to 1.5 percent of compound vitamin, 0.3 to 0.5 percent of digestion promoter, 1.5 to 2 percent of compound microorganism strain, 0.5 to 0.8 percent of corn cob meal and 90.85 to 93.82 percent of purified water.
Preferably, the composite microecological preparation consists of the following components in percentage by weight: 1.0% of ornithine, 0.8% of lysine, 0.4% of arginine, 1.4% of chitosan oligosaccharide, 0.1% of garlicin, 1.2% of compound vitamin, 0.4% of digestion promoter, 1.7% of compound microorganism strain, 0.6% of corncob meal and 92.4% of purified water.
Preferably, the vitamin complex comprises vitamin B2, vitamin C and vitamin D according to the weight ratio of 1-2: 7 to 10: 3-5; the digestion promoter is prepared from xylanase and pectinase according to a weight ratio of 5-8: 1 to 3.
Preferably, the vitamin complex consists of vitamin B2, vitamin C and vitamin D in a weight ratio of 1.5:9:4, preparing a composition; the digestion promoter is prepared from xylanase and pectinase according to a weight ratio of 7: 2.
Preferably, the complex microbial strain is prepared from enterococcus faecalis, pediococcus pentosaceus and bifidobacterium animalis according to the bacterial quantity ratio of 11-15: 7 to 9:2 to 5.
Preferably, the composite microbial strain is prepared from enterococcus faecalis, pediococcus pentosaceus and bifidobacterium animalis according to the bacterial quantity ratio of 13:8:3, the components are mixed.
Preferably, the enterococcus faecalis is cultured to a medium per gramThe number of bacteria in the culture medium is 3.2-3.5 multiplied by 10 8 (ii) a The pediococcus pentosaceus is cultured until the number of bacteria in each gram of culture medium reaches 2.3-2.6 multiplied by 10 8 (ii) a The animal bifidobacterium is cultured until the number of bacteria in each gram of culture medium reaches 3.6 to 3.8 multiplied by 10 8 。
The invention also provides a preparation method of the composite microecological preparation, which comprises the following steps:
s1, mixing and stirring water, ornithine, lysine, arginine and chitosan oligosaccharide uniformly to prepare a mixture I;
s2, adding allicin, compound vitamins and a digestion promoter into the mixture I obtained in the step S1, and uniformly mixing and stirring to prepare a mixture II;
and S3, mixing the strains in proportion, uniformly mixing the composite microbial strains and the corncob powder, adding the mixture into the mixture II obtained in the step S2, uniformly mixing and stirring, and drying at a low temperature of 6-10 ℃ for 15-20 min to obtain the corn cob composite microbial corn cob powder.
Preferably, the mixing and stirring conditions in the steps S1 and S2 are stirring at a rotating speed of 300-500 rpm for 20-30 min; the condition of uniform mixing and stirring in the step S3 is that the mixture is stirred for 30-40 min at the rotating speed of 80-120 rpm.
The invention also provides application of the composite microecological preparation as a feed additive in preparation of epidemic prevention feed for porkets.
When the composite microecological preparation is used as a feed additive, the composite microecological preparation is mixed with conventional feed according to the ratio of 6:94, and directly feeding the feed to suckling pigs,
in the research and development process of the inventor, the added ornithine, lysine and arginine can provide nutrient substances for the suckling pigs, the chitosan oligosaccharide has good water solubility, can improve the immunity of the suckling pigs and help to resist the pollution of bacteria, viruses and the like, and the corncob powder can make strains fully attached, so that the resistance of the strains to external germs is improved; a small amount of allicin is added to improve the taste of the feed and promote the suckling pigs to eat, and the compound vitamin provides multiple vitamins for the suckling pigs and fully provides nutrient substances for the suckling pigs; the digestion promoter promotes the digestion and absorption capacity of the suckling pigs on the feed, and reduces food waste; in addition, the inventor also unexpectedly finds that the allicin and the digestion promoter are added in proportion, so that the digestion and absorption effects of the suckling pigs on food can be effectively improved, the epidemic prevention function of the suckling pigs is improved, and the disease occurrence probability of the suckling pigs is reduced.
Compared with the prior art, the composite microecological preparation provided by the invention has the following advantages:
(1) The composite microecological preparation provided by the invention can supplement various nutrient substances for the suckling pigs, and improves the immunity of the suckling pigs;
(2) The corn cob powder is added into the composite microecological preparation provided by the invention, so that the composite strains are fully mixed, the defense capability of the strains on foreign bacteria and viruses is improved, and the occurrence probability of diseases is reduced;
(3) The composite microecological preparation provided by the invention is added with the components of a digestion promoter, garlicin and the like, so that the taste of the feed is improved, and the digestion and absorption functions of the suckling pigs are effectively improved.
Detailed Description
The present invention is further explained with reference to the following specific examples, but it should be noted that the following examples are only for explaining the present invention and are not intended to limit the present invention, and all technical solutions identical or similar to the present invention are within the protection scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are commercially available products.
The allicin is available from Wuhanji Industrial upgrading chemical Co., ltd; the ornithine, lysine and arginine can be purchased from Zhengzhou Baisite food additive limited; the corncob meal can be purchased from a company with limited responsibility of chatting on the Shengji agricultural science and technology development; the enterococcus faecalis (strain number: ATCC 29212) can be purchased from Changchang great wall biology, inc., nanchang, the culture medium is a common LB solid culture medium, can be purchased from Shanghai Zhengyu Biotechnology, inc., and has a product number of CM0007; pediococcus pentosaceus (strain number: ATCC 8081) and Bifidobacterium animalis (strain number: ATCC 25527) can be purchased from China institute of microbiology; the pediococcus pentosaceus is cultured by using an MRS culture medium, wherein the culture medium can be purchased from Qingdao Tuopus bioengineering limited company, and the product number is as follows: M0011B; the animal bifidobacterium is cultured by a bifidobacterium BS culture medium which can be purchased from Qingdao Haibo biotechnology limited company, and the product number is as follows: HB0394.
Example 1A Complex Microecological preparation
The composite microecological preparation comprises the following components in percentage by weight: 0.8% of ornithine, 0.5% of lysine, 0.3% of arginine, 1.2% of chitosan oligosaccharide, 0.08% of garlicin, 1% of compound vitamin, 0.3% of digestion promoter, 1.5% of compound microorganism strain, 0.5% of corncob meal and 93.82% of purified water; the compound vitamin is prepared from vitamin B2, vitamin C and vitamin D according to the weight ratio of 1:7:3, preparing; the digestion promoter is prepared from xylanase and pectinase according to a weight ratio of 5:1, preparing a composition; the compound microbial strain is prepared from enterococcus faecalis, pediococcus pentosaceus and bifidobacterium animalis according to the bacterial quantity ratio of 11:7:2, preparing a composition; preferably, the enterococcus faecalis is cultured to a bacteria count of 3.2 × 10/g culture medium 8 (ii) a The pediococcus pentosaceus is cultured until the number of bacteria in each gram of culture medium reaches 2.3 multiplied by 10 8 (ii) a The animal bifidobacterium is cultured until the number of bacteria in each gram of culture medium reaches 3.6 multiplied by 10 8 。
The preparation method of the composite microecological preparation comprises the following steps:
s1, mixing and stirring water, ornithine, lysine, arginine and chitosan oligosaccharide at the rotating speed of 300rpm for 20min until the mixture is uniformly mixed to prepare a mixture I;
s2, adding allicin, vitamin complex and a digestion promoter into the mixture I obtained in the step S1, mixing and stirring for 20min at the rotating speed of 300rpm, and uniformly mixing to prepare a mixture II;
and S3, mixing the strains in proportion, uniformly mixing the composite microbial strains and the corncob powder, adding the mixture into the mixture II obtained in the step S2, mixing and stirring at the rotating speed of 80rpm for 30min, uniformly mixing, and drying at the low temperature of 6 ℃ for 15min to obtain the corn cob composite microbial composite material.
Example 2A Complex Microecological preparation
The composite microecological preparation comprises the following components in percentage by weight: 1.2 percent of ornithine, 1.0 percent of lysine, 0.5 percent of arginine, 1.5 percent of chitosan oligosaccharide, 0.15 percent of garlicin, 1.5 percent of compound vitamin, 0.5 percent of digestion promoter, 2 percent of compound microorganism strain, 0.8 percent of corncob meal and 90.85 percent of purified water; the compound vitamin is prepared from vitamin B2, vitamin C and vitamin D according to the weight ratio of 2:10:5, preparing a composition; the digestion promoter is prepared from xylanase and pectinase according to a weight ratio of 8:3, preparing; the compound microbial strain is prepared from enterococcus faecalis, pediococcus pentosaceus and bifidobacterium animalis according to the bacterial quantity ratio of 15:9:5, preparing a composition; the enterococcus faecalis is cultured until the number of bacteria in each gram of culture medium reaches 3.5 multiplied by 10 8 (ii) a The pediococcus pentosaceus is cultured until the number of bacteria in each gram of culture medium reaches 2.6 multiplied by 10 8 (ii) a The animal bifidobacterium is cultured until the number of bacteria in each gram of culture medium reaches 3.8 multiplied by 10 8 。
The preparation method of the composite microecological preparation comprises the following steps:
s1, mixing and stirring water, ornithine, lysine, arginine and chitosan oligosaccharide at the rotating speed of 500rpm for 30min, and uniformly mixing to prepare a mixture I;
s2, adding allicin, vitamin complex and a digestion promoter into the mixture I obtained in the step S1, mixing and stirring for 30min at the rotating speed of 500rpm, and uniformly mixing to prepare a mixture II;
and S3, mixing the strains in proportion, uniformly mixing the composite microbial strains and the corncob powder, adding the mixture into the mixture II obtained in the step S2, mixing and stirring at the rotating speed of 120rpm for 40min, uniformly mixing, and drying at the low temperature of 10 ℃ for 20min to obtain the corn cob composite microbial composite material.
Example 3A Complex Microecological preparation
The composite microecological preparation comprises the following components in percentage by weight: 1.0% of ornithine, 0.8% of lysine, 0.4% of arginine, 1.4% of chitosan oligosaccharide, 0.1% of garlicin, 1.2% of compound vitamin, 0.4% of digestion promoter, 1.7% of compound microbial strain, 0.6% of corncob powder and 92.4% of purified water; the compound vitamin is prepared from vitamin B2, vitamin C and vitamin D according to the weight ratio1.5:9:4, preparing a composition; the digestion promoter is prepared from xylanase and pectinase according to a weight ratio of 7:2, preparing a composition; the compound microbial strain is prepared from enterococcus faecalis, pediococcus pentosaceus and bifidobacterium animalis according to the bacterial dose ratio of 13:8:3, preparing; the enterococcus faecalis is cultured until the number of bacteria in each gram of culture medium reaches 3.3 multiplied by 10 8 (ii) a The pediococcus pentosaceus is cultured until the number of bacteria in each gram of culture medium reaches 2.5 multiplied by 10 8 (ii) a The animal bifidobacterium is cultured until the number of bacteria in each gram of culture medium reaches 3.7 multiplied by 10 8 。
The invention also provides a preparation method of the composite microecological preparation, which comprises the following steps:
s1, mixing and stirring water, ornithine, lysine, arginine and chitosan oligosaccharide at the rotating speed of 400rpm for 25min, and uniformly mixing to prepare a mixture I;
s2, adding allicin, vitamin complex and a digestion promoter into the mixture I obtained in the step S1, mixing and stirring for 25min at the rotating speed of 400rpm, and uniformly mixing to prepare a mixture II;
and S3, mixing the strains in proportion, uniformly mixing the composite microbial strains and the corncob powder, adding the mixture into the mixture II obtained in the step S2, mixing and stirring at the rotating speed of 100rpm for 35min, uniformly mixing, and drying at the low temperature of 8 ℃ for 18min to obtain the corn cob powder.
Comparative example 1A composite Microecological preparation
The composite microecological preparation and the preparation method thereof are similar to those of example 3;
the difference from example 3 is that the digestion promoting agent in comparative example 1 is prepared from xylanase and pectinase in a weight ratio of 1: 1.
Comparative example 2A composite Microecological preparation
The composite microecological preparation and the preparation method thereof are similar to those of example 3;
the difference from example 3 is that the digestion promoting agent in comparative example 2 is xylanase.
Comparative example 3A Compound Microecological preparation
The composite microecological preparation and the preparation method thereof are similar to those of example 3;
the difference from example 3 is that comparative example 3 does not contain allicin.
Comparative example 4A composite Microecological preparation
The composite microecological preparation and the preparation method thereof are similar to those of example 3;
the difference from example 3 is that the multivitamin in comparative example 4 is composed of vitamin B2, vitamin C and vitamin D in a weight ratio of 1:1: 1.
Comparative example 5A composite Microecological preparation
The composite microecological preparation and the preparation method thereof are similar to those of example 3;
the difference from example 3 is that the composite microbial species in comparative example 5 is prepared from enterococcus faecalis, pediococcus pentosaceus and bifidobacterium animalis in a ratio of 1:1: 1.
Comparative example 6A composite Microecological preparation
The composite microecological preparation and the preparation method thereof are similar to those of example 3;
the difference from example 3 is that the complex microorganism strain in comparative example 6 is a strain prepared from pediococcus pentosaceus and bifidobacterium animalis in a ratio of 8:3, and (3).
Comparative example 7 composite microecological preparation
The composite microecological preparation and the preparation method thereof are similar to those of example 3;
the difference from example 3 is that the composite microbial species in comparative example 7 was prepared from enterococcus faecalis and pediococcus pentosaceus in a bacterial ratio of 13:18 of the composition.
Comparative example 8 composite microecological preparation
The composite microecological preparation and the preparation method thereof are similar to those of example 3;
the difference from example 3 is that the microbial species in comparative example 8 was enterococcus faecalis.
Test example 1 digestion and absorption test
1. Test samples: composite probiotics prepared in examples 1 to 3 and comparative examples 1 to 3; concentrated piglet nursing feed purchased from Henan and North agricultural big feed Co., ltd, license number: (2015) 01092;
2. test animals: 70 piglets which are born for half a month and have the weight of 15 +/-1 kg are averagely divided into 7 groups of 10 pigs, and the following requirements are met: each pig is healthy and has no hereditary diseases;
3. the test method comprises the following steps: the complex probiotics prepared in examples 1-3 and comparative examples 1-3 were added as feed additives to the piglet care concentrate, and the ratio of the two was 6:94, feeding 12kg of the test feed described in examples 1-3 and comparative examples 1-3 per group per day, feeding only the piglet nursing concentrated feed to the control group, renewing the feed per day, freely drinking water, continuously measuring the weight and the food intake 2 times per day for 10 days, calculating the average weight, the weight gain, the total food intake and the food utilization of the piglets in each group at the end of the test, and taking the average values as the final weight; daily gain and daily food intake were averaged over 10 animals per group.
4. And (3) test results: the specific test results are shown in Table 1.
Figure BDA0002701340580000081
As can be seen from Table 1, the daily gain of the experimental group, to which the feed prepared according to examples 1-3 of the present invention was added, was significantly increased and the average daily food consumption was not greatly different from that of the control group, indicating that the digestive absorption capacity of the piglets fed with the feed of examples of the present invention was higher, especially the daily gain of 736.42g in example 3 was the best example of the present invention, whereas the digestive absorption capacity of the comparative examples 1-4 was significantly decreased due to the removal of garlicin due to the change of the ingredients of the digestive promoter.
Test example 2 resistance test
1. Test samples: composite probiotics prepared in examples 1 to 3 and comparative examples 1 to 8; the concentrated piglet nursing feed purchased from the agricultural large feed limited company in the south and the south of Henan, the license number is as follows: (2015) 01092; alpha-hemolytic streptococcus, beta-hemolytic streptococcus available from Shanghai-Yizhao Biotechnology Ltd;
2. test animals: 120 male piglets, with body weight of 15 ± 1kg, were evenly divided into 12 groups, requiring: the health is good, and no obvious genetic disease exists;
3. the test method comprises the following steps: the complex probiotics prepared in examples 1-3 and comparative examples 1-3 were added as feed additives to the piglet care concentrate, and the ratio of the two was 6: 94; mixing alpha-hemolytic streptococcus and beta-hemolytic streptococcus according to the weight ratio of 1:1 mixing to obtain mixed bacterial powder.
The experiment group is divided into an experiment group and a control group, the experiment group is fed with feed added with the composite microecologics of the examples 1-3 and the comparative examples 1-8 every day, the control group is fed with common suckling pig nursing concentrated feed, and enough feed is fed every day, so that the suckling pigs can drink water freely and eat freely; meanwhile, 200mg of the mixed bacterial powder (completely eaten) is fed to each group of suckling pigs twice a day for 10 days continuously. After continuous 3 months, the immunopotentiation effect on suckling pigs was observed. The immune enhancement index is determined by measuring leukocytes in blood collected from pigs before and after administration of the "mixed bacterial powder"; the average value of each group is taken as the final value.
White blood cell change value = final white blood cell count-initial white blood cell count.
4. And (3) test results: the specific test results are shown in Table 2.
TABLE 2 comparison of the leukocyte counts of suckling pigs fed with different test samples
Figure BDA0002701340580000091
As can be seen from Table 2, the experimental groups fed with the feeds containing the complex probiotics prepared in examples 1-3 of the present invention all had higher white blood cell number reduction values, indicating that the resistance was strong, especially the resistance was strongest in example 3, which is the best example of the present invention, while the resistance was reduced to different degrees in comparative examples 1-8, which laterally reflects the synergistic effect among the components of the present invention.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims (7)

1. The composite microecological preparation is characterized by comprising the following components in percentage by weight: 0.8 to 1.2 percent of ornithine, 0.5 to 1.0 percent of lysine, 0.3 to 0.5 percent of arginine, 1.2 to 1.5 percent of chitosan oligosaccharide, 0.08 to 0.15 percent of garlicin, 1 to 1.5 percent of compound vitamin, 0.3 to 0.5 percent of digestion promoter, 1.5 to 2 percent of compound microorganism strain, 0.5 to 0.8 percent of corncob meal and 90.85 to 93.82 percent of purified water; the compound vitamin is prepared from vitamin B2, vitamin C and vitamin D according to the weight ratio of 1-2: 7 to 10: 3-5; the digestion promoter is prepared from xylanase and pectinase according to a weight ratio of 5-8: 1 to 3; the compound microbial strain is prepared from enterococcus faecalis, pediococcus pentosaceus and bifidobacterium animalis according to the bacterial quantity ratio of 11-15: 7 to 9: 2-5; the enterococcus faecalis is cultured until the number of bacteria in each gram of culture medium reaches 3.2-3.5 multiplied by 10 8 (ii) a The pediococcus pentosaceus is cultured until the number of bacteria in each gram of culture medium reaches 2.3-2.6 multiplied by 10 8 (ii) a The animal bifidobacterium is cultured until the number of bacteria in each gram of culture medium reaches 3.6 to 3.8 multiplied by 10 8 。
2. The composite microecological formulation according to claim 1, which comprises the following components in percentage by weight: 1.0% of ornithine, 0.8% of lysine, 0.4% of arginine, 1.4% of chitosan oligosaccharide, 0.1% of garlicin, 1.2% of compound vitamin, 0.4% of digestion promoter, 1.7% of compound microorganism strain, 0.6% of corncob meal and 92.4% of purified water.
3. The complex microecological formulation according to claim 1, wherein the complex vitamin is a mixture of vitamin B2, vitamin C and vitamin D in a weight ratio of 1.5:9:4, preparing a mixture; the digestion promoter is prepared from xylanase and pectinase according to a weight ratio of 7: 2.
4. The complex microbial inoculant according to claim 1, wherein the complex microbial inoculant consists of enterococcus faecalis, pediococcus pentosaceus and bifidobacterium animalis in a ratio of 13:8:3, and (3).
5. A method for preparing the composite microecological formulation according to any one of claims 1 to 4, comprising the steps of:
s1, mixing and stirring water, ornithine, lysine, arginine and chitosan oligosaccharide uniformly to prepare a mixture I;
s2, adding allicin, multivitamins and a digestion promoter into the mixture I obtained in the step S1, and uniformly mixing and stirring to prepare a mixture II;
and S3, mixing the strains in proportion, uniformly mixing the composite microbial strains and the corncob powder, adding the mixture into the mixture II obtained in the step S2, uniformly mixing and stirring, and drying at a low temperature of 6-10 ℃ for 15-20 min to obtain the corn cob composite microbial corn cob powder.
6. The preparation method according to claim 5, wherein the mixing and stirring conditions in steps S1 and S2 are stirring at 300-500 rpm for 20-30 min; the condition of uniform mixing and stirring in the step S3 is that the mixture is stirred for 30-40 min at the rotating speed of 80-120 rpm.
7. Use of the complex microbial ecological agent of claim 1 as a feed additive in the preparation of an epidemic prevention feed for porkets.
CN202011023246.2A 2020-09-25 2020-09-25 Composite microecological preparation for epidemic prevention of suckling pigs Active CN112219946B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202011023246.2A CN112219946B (en) 2020-09-25 2020-09-25 Composite microecological preparation for epidemic prevention of suckling pigs

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011023246.2A CN112219946B (en) 2020-09-25 2020-09-25 Composite microecological preparation for epidemic prevention of suckling pigs

Publications (2)

Publication Number Publication Date
CN112219946A CN112219946A (en) 2021-01-15
CN112219946B true CN112219946B (en) 2022-12-02

Family

ID=74108666

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202011023246.2A Active CN112219946B (en) 2020-09-25 2020-09-25 Composite microecological preparation for epidemic prevention of suckling pigs

Country Status (1)

Country Link
CN (1) CN112219946B (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104664106A (en) * 2013-11-29 2015-06-03 安琪酵母股份有限公司 Composite probiotic preparation product for piglets, and preparation method of composite probiotic preparation product
CN107772106A (en) * 2016-08-29 2018-03-09 成都施普诺生物技术有限公司 A kind of premixed pig and raccoon dog feeding-stuff and its production method

Also Published As

Publication number Publication date
CN112219946A (en) 2021-01-15

Similar Documents

Publication Publication Date Title
CN101971921B (en) Zinc-enriched inactivated lactobacillus microecological agent and preparation method thereof
CN110200136A (en) A kind of antibiotic-free, the weanling pig creep feed without homologous protein
CN104206789B (en) It is a kind of to be used to prevent mastitis for milk cows and the feed addictive of brucellosis and preparation method thereof
CN103907749A (en) Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof
CN101199558A (en) Compound probiotics for poultry and preparing method thereof
CN101878858A (en) Microecological composite growth-promoting feed additive for fish
CN102406069A (en) Fermented feed special for piglets and preparation method thereof
CN101530160B (en) Preparation method of microecological preparation of lactobacillus
WO2017012573A1 (en) Bacillus pumilus strain and microbial ecological combined agent thereof for weaned piglet
CN107853461A (en) A kind of Rosa roxburghii slag fermentation nonreactive feed and preparation method thereof
CN102370054A (en) Immune improved feed additive premix and using method thereof
CN102696917A (en) Pork pig fermented feed and preparation method thereof
CN112136965A (en) Immunity-enhancing and growth-promoting fermented Chinese herbal medicine feed additive and preparation method thereof
CN110892944A (en) Weaned pig feed additive capable of replacing antibiotics and application thereof
CN102925374B (en) Saccharomyces cerevisiae new strains, and the feed addictive containing it, batch
KR100857771B1 (en) Compositions for addition to feed for fish comprising Bacillus polyfermenticus, Bacillus licheniformis and Saccharomyces serevisiae
CN110200156A (en) It is a kind of improve feed intake piglet mixed feed additive and application
CN105285327A (en) Selenium-enriched lactic acid bacterium biological feed additive and preparation method thereof
CN106819625A (en) A kind of mandarin fish fermented feed and preparation method thereof
CN112219946B (en) Composite microecological preparation for epidemic prevention of suckling pigs
CN115211492B (en) Stress-resistant growth-promoting stress-relieving medicine for livestock and poultry, and preparation method and application thereof
CN106359992A (en) Seaweed additive aiming at diarrhea feed for piglets
CN101095465B (en) Application of low-polyxylose complicated preparation in the preparing of feedstuff that is capable of enhancing immunity of marine animals
CN109645220A (en) A kind of composite microbial feed additive and preparation method thereof
CN108419948A (en) A kind of feed promoting Zhu-si broiler intestinal health

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant