CN102370054A - Immune improved feed additive premix and using method thereof - Google Patents
Immune improved feed additive premix and using method thereof Download PDFInfo
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Abstract
The invention provides an immune improved feed additive premix, which consists of the following components in percentage by mass: 5 to 10 percent of low-molecular-weight chitosan, 5 to 15 percent of bacillus natto, 5 to 15 percent of bacillus licheniformis, 5 to 15 percent of bacillus subtilis, and 45 to 80 percent of carrier. In the using method of the immune improved feed additive premix, the mixing proportion of the immune improved feed additive premix to the feed is 0.3:1000 (kg)-1:1000 (kg). According to the method, the immunity of an animal body is regulated by using a special combination effect of the low-molecular-weight chitosan and beneficial bacteria, so that the disease resistance is improved, the production performance of an animal is improved, the growing potential of the animal is excited, and remarkable effect is obtained. By virtue of scientific matching, antibiotics are effectively replaced by the premix, drug resistance, drug tolerance, drug residues and other toxic or side effects in long-term use of the antibiotics are avoided, and sustainable development of livestock breeding and feed industry is facilitated.
Description
Technical field
The invention belongs to technical field of animal, particularly a kind of immunity improvement feedstuff additive premix compound and method for using.
Background technology
At present, the medium-term and long-term high dose of feed adds antibiotic as medicated feed additive, for guaranteeing cultivated animals health solid guarantee is provided, and has also obtained certain effect.But long-term heavy dose of antibiotic adds the potential hazard that cultivated animals itself is caused and through food the phenomenon that human health constitutes a threat to is more and more caused our attention.For at the safe and sanitary that guarantees to excite its growth potential on the healthy growth basis of cultivated animals better and guarantee animal product, seek the top priority that green, efficient, safe antibiotic substitute have become various countries Animal nutrition educational circles.
Summary of the invention
The objective of the invention is to overcome the deficiency of above-mentioned existence; A kind of immunity improvement feedstuff additive premix compound is provided and is used for animal feeding; Be used for substituting the antibiotic medicine additive; Regulate the specific immunity and the nospecific immunity of cultivated animals, improve its resistance, guarantee the health of cultivated animals and farthest excite its growth potential disease.
The objective of the invention is to accomplish through following technical scheme; A kind of immunity improvement feedstuff additive premix compound, it is made up of chitosan oligomer 5-10% (mass percent), bafillus natto 5-15% (mass percent), bacillus licheniformis 5-15% (mass percent), bacillus subtilis 5-15% (mass percent) and carrier 45-80% (mass percent).
Described chitosan oligomer is 5-10% (mass percent), and shitosan is a kind of solubility glycosaminoglycan, is obtained by the chitin deacetylase base.Chitin extensively is present in the shell of cell membrane and insect of shell, fungi (saccharomycete, filamentous fungi) of shellfish (shrimp, crab).Because shitosan has functions such as significant immunological regulation and antibacterial bacteriostatic, causes various countries scholar's concern in recent years.If along with the deep discovery of research is reduced to the molecular weight of shitosan between 1～100,000 scope; Then various functional effects can obviously improve; Chitosan oligomer promotes and conditioning agent to have the functions such as propagation of antibacterial bacteriostatic, reduction cholesterol, adjusting fat metabolism, promotion intestinal beneficial bacterium as a kind of immunity.Chitosan oligomer is with wide material sources, pure natural, safe and efficient and receive people's common concern and research, and its range of application expands to feedstuff industry rapidly.
Described bafillus natto is 5-15% (mass percent); Bafillus natto is the aerobic probio, except that tolerance general feeds granulation condition, behind the feeding animals in stomach rudiment rapidly; And at short notice the intestinal environment change is anaerobic state; The growth, the propagation that help lonely property Miyarisan and the absolute anaerobic bacterias such as property Bifidobacterium, Bacillus acidi lactici that are sick of, and then the secretion butyric acid reaches the effect of consistent pathogen with acetic acid, and can effectively suppress the growth of aerobic harmful bacterium such as enterobacteria and enterococcus in the enteron aisle; Promote the growth of host's normal intestinal flora, keep the balance of little ecology.On the other hand, bafillus natto produces useful metabolite at digestive ferment and vitamins such as animal intestinal endocrine protease, amylase, thereby improves animal to the digesting and assimilating of feed nutrient, and improves livestock and poultry feed conversion ratio and production performance.
Described bacillus licheniformis is 5-15% (mass percent); After the bacillus licheniformis viable bacteria gets into enteron aisle, pathogenic bacteria such as staphylococcus, yeast-like fungus there is antagonism, and Bifidobacterium, Bacillus acidi lactici, bacteroid, digestibility streptococcus are had the growth of promotion effect, reach therapeutic purposes thereby can adjust flora imbalance.Can impel body to produce antibacterial substance, kill pathogenic bacteria.Make the enteron aisle anoxic through taking the oxygen biological effect by force in addition, help the growth of a large amount of anaerobic bacterias, cause the enteron aisle low-oxygen environment, the growth and breedings of wholesome anaerobic bacterias such as the Bifidobacterium in the enteron aisle, Bacillus acidi lactici, bacteroid, peptostreptococcus is had facilitation; Staphylococcus, white are read pathogenic bacteria such as coccus, yeast-like fungus antagonism is then arranged.Can adjust intestinal bacilli illness through this double action, keep body intestinal microecology balance, thereby intestines problem is reached the purpose of treatment and prevention.
Described bacillus subtilis is 5-15% (mass percent); The subtilin that produces in the bacillus subtilis thalli growth process, polymyxins, nystatin, gramicidins isoreactivity material, these active materials have the obvious suppression effect to the conditioned pathogen of pathogenic bacteria or autogenous infection; Get in the body back oxygen consumed rapidly, cause the enteron aisle hypoxemia, promote useful anaerobic bacteria growth, and produce organic acid such as lactic acid, reduce the enteron aisle pH value, suppress other pathogenic bacteria growth indirectly; Stimulate growing of animal immune organ, activate T, bone-marrow-derived lymphocyte, improve immunoglobulin (Ig) and antibody horizontal, strengthen cellular immunity and humoral immune function, improve herd immunity; Bacillus subtilis thalline self synthesizes enzymes such as a-amylase, protease, lipase, cellulase, in alimentary canal with animal body in digestive enzymes together play a role; Multiple B family vitamins such as ability synthetic vitamin B1, B2, B6, nicotinic acid, the activity of interferon and macrophage in the raising animal body.
Described carrier can adopt conventional bio-additive carrier.
Described chitosan oligomer molecular weight is 1-10 ten thousand; Average fineness is at the 15-25 micron; Employed chitosan oligomer is to adopt physics mode degrade chitosan gained among the present invention; This method is directly cut off the shitosan macromolecular chain; The chitosan oligomer that this mode is degraded has and does not change original shitosan natural molecule structure, do not lose the amino on the strand, characteristics such as do not have that other impurity, molecular weight are balanced, effects such as immunological regulation and antibacterial bacteriostatic are more remarkable; Adopting its average fineness of superfine powder crushing technology can be 15 microns, has improved its absorption and utilization rate in enteron aisle greatly.
Described bafillus natto, bacillus licheniformis, bacillus subtilis contain the viable bacteria number average and are no less than 10,000,000,000/gram, gemma rate＞85%.
The mixed proportion of described immunity improvement feedstuff additive premix compound and feed is 0.3: 1000 (kilogram)-1: 1000 (kilogram).
The present invention improves physique and the premunition of animal through the immunity of using chitosan oligomer and strengthening and regulate animal self; Composite beneficial bacterium plays cooperative effect simultaneously, overcomes the defective of using single preparation; Come effectively to replace antibiotic; Thereby reach health care and growth promoting function, improve survival rate, effectively avoid animal product medicament residue problem cultivated animals; Immunity improvement feedstuff additive premix compound provided by the invention has following beneficial effect:
1, stimulates the growth of animal immune organ, effectively regulate body immune system, improve premunition;
2, in feed, add these article; Avoided using the harmful effect of antibiotic to other microbiologic populations in the animal alimentary canal; Significantly regulate the ecological balance of intestinal microflora; Can also promote beneficial microorganism metabolism in the enteron aisle, the resistance to the action of a drug that can avoid using antibiotic for a long time simultaneously and occurred, drug resistance, toxic and side effect such as residual;
3, effectively alleviate the infringement of medicine, heavy metal, alleviate the burden of liver, improve the activity of enzyme in the liver, strengthen liver detoxification and nutrition conversion function liver;
4, significantly reduce cholesterol level in the animal product, reduce abdomen fat content, improve the lean meat percentage of pig, improve meat;
5, the science compatibility of chitosan oligomer and beneficial bacterium preparation has the complementary gain effect; Can improve digestion and the absorption rate of animal, effectively excite the growth potential of animal, promote its growth feed; Improve in the increasing day by day of animal better and feed conversion rate, increase economic efficiency.
Description of drawings
Fig. 1 is that immunity improvement feedstuff additive premix compound adding feed of the present invention influences figure to the weanling pig daily gain;
Fig. 2 is that immunity improvement feedstuff additive premix compound adding feed of the present invention influences figure to the weaning pigs anharmonic ratio;
Fig. 3 is that immunity improvement feedstuff additive premix compound adding feed of the present invention influences figure to the diarrhea of weaned piglets rate;
Fig. 4 is that immunity improvement feedstuff additive premix compound adding feed of the present invention influences figure to the growing swine daily gain;
Fig. 5 be immunity improvement feedstuff additive premix compound of the present invention add feed to growing swine day grazing rate influence figure;
Fig. 6 is that immunity improvement feedstuff additive premix compound adding feed of the present invention influences figure to growing swine material anharmonic ratio;
Fig. 7 is that immunity improvement feedstuff additive premix compound adding feed of the present invention influences figure to the fryer daily gain;
Fig. 8 is that immunity improvement feedstuff additive premix compound adding feed of the present invention influences figure to the broiler fodder conversion ratio;
Fig. 9 is that immunity improvement feedstuff additive premix compound adding feed of the present invention influences figure to the broiler fodder death rate;
Figure 10 is that immunity improvement feedstuff additive premix compound of the present invention adds the growth effect table of feed to grass carp;
Figure 11 is that immunity improvement feedstuff additive premix compound adding feed of the present invention influences table to grass carp liver enzyme.
The specific embodiment
A kind of immunity improvement feedstuff additive premix compound, it is made up of chitosan oligomer 5-10% (mass percent), bafillus natto 5-15% (mass percent), bacillus licheniformis 5-15% (mass percent), bacillus subtilis 5-15% (mass percent) and carrier 45-80% (mass percent).
Described chitosan oligomer is 8% (mass percent); Chitosan oligomer promotes and conditioning agent to have the functions such as propagation of antibacterial bacteriostatic, reduction cholesterol, adjusting fat metabolism, promotion intestinal beneficial bacterium as a kind of immunity.
Described bafillus natto is 10% (mass percent); Can effectively suppress the growth of aerobic harmful bacterium such as enterobacteria and enterococcus in the enteron aisle; Promote the growth of host's normal intestinal flora; The balance that keeps little ecology can also improve animal to the digesting and assimilating of feed nutrient, and improves livestock and poultry feed conversion ratio and production performance.
Described bacillus licheniformis is 10% (mass percent); Can adjust intestinal bacilli illness, keep body intestinal microecology balance, thereby intestines problem is reached the purpose of treatment and prevention.
Described bacillus subtilis is 10% (mass percent); Get in the body back oxygen consumed rapidly, cause the enteron aisle hypoxemia, promote useful anaerobic bacteria growth, and produce organic acid such as lactic acid, reduce the enteron aisle pH value, suppress other pathogenic bacteria growth indirectly; Stimulate growing of animal immune organ, activate T, bone-marrow-derived lymphocyte, improve immunoglobulin (Ig) and antibody horizontal, strengthen cellular immunity and humoral immune function, improve herd immunity.
Described carrier can adopt conventional bio-additive carrier.
Described chitosan oligomer molecular weight is 1-10 ten thousand; Average fineness is at the 15-25 micron.
Described bafillus natto, bacillus licheniformis, bacillus subtilis contain the viable bacteria number average and are no less than 10,000,000,000/gram, gemma rate＞85%.
The mixed proportion of described immunity improvement feedstuff additive premix compound and feed is 0.3: 1000 (kilogram)-1: 1000 (kilogram).The concrete method for using of this immunity improved additives premix is: these article are mixed or spice with feed, and different animal adopts different proportionings at different physiological stages: newborn piglet 0.5～1.0: 1000 (kilogram); Growing-finishing pig 0.3～0.5: 1000 (kilograms); Sow 0.3～0.5: 1000 (kilograms); Birds 0.3～0.5: 1000 (kilogram) such as fryer, laying hen, duck; Aquatic livestock 0.3～0.5: 1000 (kilograms).
Embodiment 1: shown in Fig. 1-3: the application of immunity improvement feedstuff additive premix compound in piglet produces
This test is carried out on large-scale pig farm in Chongqing, the identical genotype of test and Selection, wean age in days be 72 of " DLY " three way cross weanling pigs of 28 ± 2 days, the research of having carried out.The control group fed basal diet is handled 1 group on the basal diet basis, adding this additive premix 0.5 ‰, handles 2 groups and on the basal diet basis, adds this additive premix 1.0 ‰, 28 days experimental periods.Result of the test shows: handle 1 group, handle 2 groups of average daily gains and improve 8% and 15% respectively than control group, the material anharmonic ratio reduces by 12% and 20% respectively, and diarrhea rate reduces by 46% and 63% respectively.
Embodiment 2: shown in Fig. 4-6: the application of immunity improvement feedstuff additive premix compound in growing swine is produced
144 70 age in days two-way cross of experiment selected pig (Rongchang County * Da Bai), be divided into 3 processing at random according to factors such as body weight, sexes, each handles 6 repetitions, and each repeats 8 pigs, and public female half and half.The control group fed basal diet is handled 1 group and on the basal diet basis, is added this additive premix 0.3 ‰, handles 2 groups and on the basal diet basis, adds this additive premix 0.5 ‰, 30 days experimental periods.Result of the test shows: handle 1 group, handle 2 groups of average daily gains and improve 24% and 36% respectively than control group, daily ingestion amount does not have notable difference, and the material anharmonic ratio reduces by 28% and 30% respectively.
Embodiment 3: shown in Fig. 7-9: the application of immunity improvement feedstuff additive premix compound in fryer is produced
Select healthy AA broiler chicks 600 plumages for use; Be divided into 3 processing at random; Each handles 8 repetitions, and processed group 1 is added this additive premix 0.3 ‰ on the basal diet basis, and processed group 2 is added this additive premix 0.5 ‰ on the basal diet basis; The plain 3.3mg/kg of the sticking bar of control group fed basal diet+Bacitracin Zinc 16.7mg/kg and sulfuric acid, experimental period is 42 days.Result of the test shows: handle 1,2 group of average daily gain and improve 7% and 16% respectively than the antibiotic control group, feed conversion rate does not have significant difference, and the death rate reduces by 6% and 8% respectively.
Embodiment 4: shown in Figure 10-11: the application of immunity improvement feedstuff additive premix compound in grass carp
Counterpoise 132 gram grass carp kinds 540 are trailed machine be divided into 3 processing (3 repetitions of every processing).Feeding respectively contains three kinds of feeds of this immunity improvement feedstuff additive premix compound 0,300g/T, 500g/T, is designated as respectively and handles 1, handles 2, handles 3.Carry out cage culture in 70 days test.The result shows: handle 3 daily gains and specific growth rate and improve respectively and reach 13.53% and 12% than handling 1; Rate of body weight gain improves (P＜0.05) than handling 1 work, and feed coefficient significantly reduces (P＜0.05).The interpolation of this feedstuff additive premix compound does not all have appreciable impact (P＞0.05) to grass carp catalase and glutamic-oxalacetic transaminease; And add 300g/T, the specific activity control group of the amount of MDA and glutamic-oxalacetic transaminease be significantly increased (P＜0.05).
In sum, can know the immunity of the present invention through using chitosan oligomer and beneficial bacterium specific combination effect to regulate animal body in conjunction with above each embodiment; Improve premunition; Improve its production performance, excite the growth potential of animal, obtained significant effect.Through scientific compatibility, thereby effectively replace antibiotic, the resistance to the action of a drug of having avoided long-term use antibiotic to be occurred, drug resistance, toxic and side effect such as residual help the sustainable development of aquaculture and feed industry.
Claims (8)
1. immunity improvement feedstuff additive premix compound is characterized in that it is made up of chitosan oligomer 5-10% (mass percent), bafillus natto 5-15% (mass percent), bacillus licheniformis 5-15% (mass percent), bacillus subtilis 5-15% (mass percent) and carrier 45-80% (mass percent).
2. immunity improvement feedstuff additive premix compound according to claim 1 is characterized in that described chitosan oligomer is 8% (mass percent).
3. immunity improvement feedstuff additive premix compound according to claim 1 is characterized in that described bafillus natto is 10% (mass percent).
4. immunity improvement feedstuff additive premix compound according to claim 1 is characterized in that described bacillus licheniformis is 10% (mass percent).
5. immunity improvement feedstuff additive premix compound according to claim 1 is characterized in that described bacillus subtilis is 10% (mass percent).
6. immunity improvement feedstuff additive premix compound according to claim 1 and 2 is characterized in that described chitosan oligomer molecular weight is 1-10 ten thousand; Average fineness is at the 15-25 micron.
7. according to claim 1 or 3 or 4 or 5 or 6 described immunity improvement feedstuff additive premix compounds, it is characterized in that described bafillus natto, bacillus licheniformis, bacillus subtilis contain the viable bacteria number average and be no less than 10,000,000,000/gram, gemma rate＞85%.
8. the method for using like the described immunity improvement of claim 1 to 5 feedstuff additive premix compound is characterized in that the mixed proportion of described immunity improvement feedstuff additive premix compound and feed is 0.3: 1000 (kilogram)-1: 1000 (kilogram).
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| CN102805209A (en) * | 2012-08-30 | 2012-12-05 | 湖南省微生物研究所 | Synbiotics feed additive for beasts and birds and application thereof |
| CN104543404A (en) * | 2015-01-17 | 2015-04-29 | 东北农业大学 | Natural compound feed additive for removing veterinary drug residues |
| CN107468714A (en) * | 2017-07-28 | 2017-12-15 | 南京农业大学 | A kind of compound formulation for the sterilization of aquatic livestock body surface and its preparation method and application |
| CN107927328A (en) * | 2017-04-26 | 2018-04-20 | 山东弘德农牧科技有限公司 | A kind of preparation method and applications of feed composite microecologic agent |
| CN108821412A (en) * | 2018-07-02 | 2018-11-16 | 河南牧业经济学院 | A kind of improver of water quality and preparation method thereof for aquaculture |
| CN110037197A (en) * | 2019-05-07 | 2019-07-23 | 合肥亚博农牧有限公司 | A kind of duck feed formula and production method |
| CN112544509A (en) * | 2020-12-10 | 2021-03-26 | 珠海市现代农业发展中心(珠海市金湾区台湾农民创业园管理委员会、珠海市农渔业科研与推广中心) | Artificial breeding method of eleutheronema tetradactylum |
| CN114097937A (en) * | 2020-08-26 | 2022-03-01 | 河北科星药业有限公司 | Feed additive for preventing diarrhea and improving immunity and preparation method and application thereof |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102805209A (en) * | 2012-08-30 | 2012-12-05 | 湖南省微生物研究所 | Synbiotics feed additive for beasts and birds and application thereof |
| CN102805209B (en) * | 2012-08-30 | 2014-05-28 | 湖南省微生物研究所 | Synbiotics feed additive for beasts and birds and application thereof |
| CN104543404A (en) * | 2015-01-17 | 2015-04-29 | 东北农业大学 | Natural compound feed additive for removing veterinary drug residues |
| CN107927328A (en) * | 2017-04-26 | 2018-04-20 | 山东弘德农牧科技有限公司 | A kind of preparation method and applications of feed composite microecologic agent |
| CN107468714A (en) * | 2017-07-28 | 2017-12-15 | 南京农业大学 | A kind of compound formulation for the sterilization of aquatic livestock body surface and its preparation method and application |
| CN107468714B (en) * | 2017-07-28 | 2020-04-14 | 南京农业大学 | Compound preparation for disinfecting body surfaces of aquatic animals and preparation method and application thereof |
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| CN110037197A (en) * | 2019-05-07 | 2019-07-23 | 合肥亚博农牧有限公司 | A kind of duck feed formula and production method |
| CN114097937A (en) * | 2020-08-26 | 2022-03-01 | 河北科星药业有限公司 | Feed additive for preventing diarrhea and improving immunity and preparation method and application thereof |
| CN112544509A (en) * | 2020-12-10 | 2021-03-26 | 珠海市现代农业发展中心(珠海市金湾区台湾农民创业园管理委员会、珠海市农渔业科研与推广中心) | Artificial breeding method of eleutheronema tetradactylum |
| CN112544509B (en) * | 2020-12-10 | 2022-05-27 | 珠海市现代农业发展中心(珠海市金湾区台湾农民创业园管理委员会、珠海市农渔业科研与推广中心) | Artificial breeding method of eleutheronema tetradactylum |
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Application publication date: 20120314 |