CN104770596A - Aquatic animal intestinal regulator and application thereof - Google Patents
Aquatic animal intestinal regulator and application thereof Download PDFInfo
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- CN104770596A CN104770596A CN201510162168.7A CN201510162168A CN104770596A CN 104770596 A CN104770596 A CN 104770596A CN 201510162168 A CN201510162168 A CN 201510162168A CN 104770596 A CN104770596 A CN 104770596A
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Abstract
The present invention discloses an aquatic animal intestinal regulator and an application thereof. The aquatic animal intestinal regulator is prepared from the following materials in parts by weight: 15-30 parts of strains, 20-40 parts of soluble starch, 20-40 parts of sugar, 10-20 parts vegetable protein powder, and 5-15 parts of small molecule organic sodium salt. The regulator can be added to feed, and can provide beneficial bacteria for intestinal after devoured by aquatic animals; the regulator also can provide a conventional carbon source and a special carbon source which has an inhibiting effect to some micro-organisms. The regulator is rich in fermentation vegetable protein of a variety of microbial metabolites, can promote colonization and breeding of beneficial microbial communities, and can suppress growth of harmful microorganisms, so aquatic animals can maintain intestinal flora balance. Experiments prove that the regulator can improve the activity and diversity of the aquatic animal intestinal microflora, increase the number of the beneficial bacteria such as Bacillus, and reduce the number of harmful bacteria such as Aeromonas.
Description
Technical field
The invention belongs to the biological technical field in aquaculture, be specifically related to a kind of aquatic products enteric modulators and application thereof.
Background technology
Enteron aisle is the forefront of body defenses, and be maximum immune organ, it has three zones for body, digests and assimilates, immune defense and nerve modulation, and have the immunocyte of nearly half to be attached on enteron aisle, the antibody sources of 80% is in enteron aisle.But compared with being equivalent to the long 5-6 of body enteron aisle doubly with terrestrial animal, the enteron aisle of aquatic livestock is relatively short, mostly is about 2 times that body is long, what have is even long than body also short.Therefore, regulate aquatic livestock intestinal health particularly important, and want targetedly.
Summary of the invention
For solving the problem, the present invention is directed to aquatic livestock enteron aisle feature, start with from gut flora regulation and control, a kind of aquatic livestock enteric modulators is provided, it can improve aquatic livestock intestinal microflora diversity and active function, it can increase the beneficial bacterium quantity such as bacillus, reduces the harmful bacteria quantity such as Aeromonas.
Object of the present invention is realized by following technical scheme: a kind of aquatic livestock enteric modulators, it is characterized in that: calculate formula by weight and comprise: bacterial classification 15-30 part, soluble starch 20-40 part, sugared 20-40 part, plant protein powder 10-20 part, small molecular organic acid sodium salt 5-15 part.
Calculate by weight, described formula comprises: bacterial classification 18-25 part, soluble starch 20-30 part, sugared 30-40 part, plant protein powder 10-15 part, small molecular organic acid sodium salt 8-12 part.
Described bacterial classification is by dregs of beans sort of quyi 0.15-0.25kg, dregs of beans 5-7kg, wheat bran 2.5-3.5kg, rice bran 0.8-1.2kg, brown sugar 0.2-0.4kg, water 6-8kg, half anaerobic fermentation is made, fermentation temperature controls at 25-35 DEG C, ferment summer 2-3 days, ferment winter 5-7 days, after fermentation, and dried and crushed.
Described bacterial classification be selected from bacillus, photosynthetic bacteria, lactic acid bacteria or saccharomycetic more than one.
Described soluble starch is selected from one or more mixing in rice starch, cornstarch, little rice starch, potato starch or starch from sweet potato.
Described sugar is one or more mixing in brown sugar, soy molasses, cane molasses or glucose.
Described vegetable protein is the bean cake powder after fermentation.
Described small molecular organic acid sodium salt is selected from one or more mixing in sodium acetate, sodium propionate, sodium butyrate or sodium lactate.
Above-mentioned aquatic livestock enteric modulators is applied to prepares aquatic animal feed additive.
Aquatic livestock enteric modulators is applied to aquatic animal feed additive, using method comprises: aquatic livestock enteric modulators mixes with the weight ratio of water by 1:8-12 by (1), half anaerobic fermentation, fermentation temperature controls at 25-35 DEG C, ferment summer 2-3 days, ferment winter 5-7 days, obtains zymotic fluid; (2) every 100kg aquatic animal feed adds zymotic fluid 2-5kg, mixes and feeds aquatic livestock.
Beneficial effect of the present invention is: after aquatic livestock swallows, bacteria group can be provided for its enteron aisle, conventional carbon source is provided simultaneously and has inhibiting extraordinary carbon source to some miniature organism, and be rich in the fermented vegetable protein of multiple-microorganism metabolite, promote that effective microorganisms makes a decision to plant to breed, suppress harmful microorganism growth, aquatic livestock is made to keep gut flora balance, experiment proof can improve aquatic livestock intestinal microflora activity and diversity, increase the beneficial bacterium quantity such as bacillus, reduce the harmful bacteria quantity such as Aeromonas.
Accompanying drawing explanation
Fig. 1 is fish body enteron aisle Aeromonas number change curve (Y-axis is bacterial population logCFU) in contrast test of the present invention;
Fig. 2 is fish body enteron aisle bacillus change curve (Y-axis is bacterial population logCFU) in contrast test of the present invention;
Fig. 3 is fish body intestinal microflora functional diversity H change curve (Y-axis is H) in contrast test of the present invention.
Detailed description of the invention
The present invention is a kind of aquatic livestock enteric modulators, calculates formula by weight and comprises: bacterial classification 15-30 part, soluble starch 20-40 part, sugared 20-40 part, plant protein powder 10-20 part, small molecular organic acid sodium salt 5-15 part.Preferably, formula comprises: bacterial classification 18-25 part, soluble starch 20-30 part, sugared 30-40 part, plant protein powder 10-15 part, small molecular organic acid sodium salt 8-12 part.Most preferred, calculate by weight, described formula comprises: bacterial classification 20 parts, soluble starch 25 parts, sugar 35 parts, plant protein powder 10 parts, small molecular organic acid sodium salt 10 parts.
Wherein, bacterial classification is by dregs of beans sort of quyi 0.15-0.25kg, dregs of beans 5-7kg, wheat bran 2.5-3.5kg, rice bran 0.8-1.2kg, brown sugar 0.2-0.4kg, water 6-8kg, half anaerobic fermentation is made, fermentation temperature controls at 25-35 DEG C, and ferment summer 2-3 days, and ferment winter 5-7 days, after fermentation, dried and crushed.Or, bacterial classification be selected from bacillus, photosynthetic bacteria, lactic acid bacteria or saccharomycetic more than one, also directly can buy the commercially available mixed bacteria including these mushrooms.Soluble starch is selected from one or more mixing in rice starch, cornstarch, little rice starch, potato starch or starch from sweet potato.Sugar is one or more mixing in brown sugar, soy molasses, cane molasses or glucose.Vegetable protein is the bean cake powder after fermentation.Small molecular organic acid sodium salt is selected from one or more mixing in sodium acetate, sodium propionate, sodium butyrate or sodium lactate.
Above-mentioned aquatic livestock enteric modulators can be applied to prepares aquatic animal feed additive.Using method comprises the following steps: aquatic livestock enteric modulators mixes with the weight ratio of water by 1:8-12 by (1), half anaerobic fermentation, and fermentation temperature controls at 25-35 DEG C, and ferment summer 2-3 days, and ferment winter 5-7 days, obtains zymotic fluid; (2) every 100kg aquatic animal feed adds zymotic fluid 2-5kg, mixes and feeds aquatic livestock.
Below in conjunction with embodiment, the present invention is further elaborated, but the present invention is not limited to this specific examples.Embodiment 1
Prepared by bacterial classification: dregs of beans sort of quyi 0.2kg, dregs of beans 6kg, wheat bran 3kg, rice bran 1kg, brown sugar 0.3kg, water 7kg, half anaerobic fermentation, and temperature is at 30 DEG C, and dry after fermentation in 3 days of fermenting, pulverized 80 mesh sieves, vacuum packaging is stand-by.
Prepared by enteric modulators: count by weight, bacterial classification 20 parts, corn soluble starch 25 parts, 35 parts, brown sugar, fermented bean dregs 10 parts, sodium propionate 10 parts.After raw material stirs, pulverized 80 mesh sieves, vacuum packaging is stand-by.
Use: take 1kg enteric modulators, add 10kg water, 5-6 month, half anaerobic fermentation 3 days.During use, every 100kg feed adds zymotic fluid 5kg and mixes hello aquatic livestock, and unspent zymotic fluid sealing is preserved, storage life 12 months.
Contrast test:
The grass carp seed rearing phase: Huangpu, Zhongshan City, Guangdong Province grass carp plant 6 mouthfuls of ponds, in June, 2014 puts spray in a suitable place to breed, July 1 started, wherein 3 mouthfuls of ponds regularly use enteric modulators, within every 10 days, use 1 time, be used in conjunction 3 days, monitoring fish body enteron aisle bacillus number, its monad number and functional diversity, result as shown in Figure 1-Figure 3 at every turn.Monitoring result shows, enteric modulators can regulate and control intestinal microflora preferably, suppresses the burst of Aeromonas to increase, improves beneficial microbe group quantity, strengthen Microbial functional diversity.
Embodiment 2
Prepared by enteric modulators: count by weight, bacterial classification (being made up of the bacillus of ratio 1:1 and photosynthetic bacteria) 25 parts, rice starch 25 parts, cane molasses 30 parts, fermented bean dregs 18 parts, acetic acid sodium salt 8 parts.After raw material stirs, pulverized 80 mesh sieves, vacuum packaging is stand-by.With embodiment 1 during use.
Embodiment 3
Prepared by enteric modulators: count by weight, bacterial classification (being made up of the bacillus of ratio 1:1:1:1, photosynthetic bacteria, lactic acid bacteria and saccharomycete) 20 parts, rice starch 20 parts, cane molasses 30 parts, fermented bean dregs 15 parts, acetic acid sodium salt 15 parts.After raw material stirs, pulverized 80 mesh sieves, vacuum packaging is stand-by.With embodiment 1 during use.
Embodiment 4
Prepared by enteric modulators: count by weight, bacterial classification (with embodiment 1) 30 parts, starch from sweet potato 40 parts, 40 parts, brown sugar, fermented bean dregs 20 parts, lactylate salt 15 parts.After raw material stirs, pulverized 80 mesh sieves, vacuum packaging is stand-by.With embodiment 1 during use.
Above-mentioned detailed description of aquatic livestock enteric modulators being carried out with reference to embodiment; illustrative instead of determinate; several embodiments can be listed according to institute's limited range, therefore in the change do not departed under general plotting of the present invention and amendment, should belong within protection scope of the present invention.
Claims (10)
1. an aquatic livestock enteric modulators, is characterized in that: calculate formula by weight and comprise: bacterial classification 15-30 part, soluble starch 20-40 part, sugared 20-40 part, plant protein powder 10-20 part, small molecular organic acid sodium salt 5-15 part.
2. aquatic livestock enteric modulators according to claim 1, is characterized in that: calculate by weight, and described formula comprises: bacterial classification 18-25 part, soluble starch 20-30 part, sugar 30-40 part, plant protein powder 10-15 part, small molecular organic acid sodium salt 8-12 part.
3. aquatic livestock enteric modulators according to claim 1, it is characterized in that: described bacterial classification is by dregs of beans sort of quyi 0.15-0.25kg, dregs of beans 5-7kg, wheat bran 2.5-3.5kg, rice bran 0.8-1.2kg, brown sugar 0.2-0.4kg, water 6-8kg, half anaerobic fermentation is made, fermentation temperature controls at 25-35 DEG C, and ferment summer 2-3 days, and ferment winter 5-7 days, after fermentation, dried and crushed.
4. aquatic livestock enteric modulators according to claim 1, is characterized in that: described bacterial classification be selected from bacillus, photosynthetic bacteria, lactic acid bacteria or saccharomycetic more than one.
5. aquatic livestock enteric modulators according to claim 1, is characterized in that: described soluble starch is selected from one or more mixing in rice starch, cornstarch, little rice starch, potato starch or starch from sweet potato.
6. aquatic livestock enteric modulators according to claim 1, is characterized in that: described sugar is one or more mixing in brown sugar, soy molasses, cane molasses or glucose.
7. aquatic livestock enteric modulators according to claim 1, is characterized in that: described vegetable protein is the bean cake powder after fermentation.
8. aquatic livestock enteric modulators according to claim 1, is characterized in that: described small molecular organic acid sodium salt is selected from one or more mixing in sodium acetate, sodium propionate, sodium butyrate or sodium lactate.
9. aquatic livestock enteric modulators described in claim 1-8 is applied to and prepares aquatic animal feed additive.
10. aquatic livestock enteric modulators is applied to aquatic animal feed additive according to claim 9, using method comprises: aquatic livestock enteric modulators mixes with the weight ratio of water by 1:8-12 by (1), half anaerobic fermentation, fermentation temperature controls at 25-35 DEG C, ferment summer 2-3 days, ferment winter 5-7 days, obtains zymotic fluid; (2) every 100kg aquatic animal feed adds zymotic fluid 2-5kg, mixes and feeds aquatic livestock.
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Cited By (5)
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| CN106036197A (en) * | 2016-05-25 | 2016-10-26 | 中国水产科学研究院黑龙江水产研究所 | Fish-meal-free sturgeon feed additive, preparation method and use method thereof |
| CN108013213A (en) * | 2018-02-07 | 2018-05-11 | 高嘉泰 | A kind of moist microorganism particle fermented feed for aquatic livestock and preparation method thereof |
| CN109275800A (en) * | 2017-07-20 | 2019-01-29 | 无锡三智生物科技有限公司 | It is a kind of for protecting the feed addictive of aquatic livestock intestinal health |
| CN110384111A (en) * | 2019-07-18 | 2019-10-29 | 无锡中顺生物技术有限公司 | A kind of synergist of lactic acid bacteria and its application |
| CN114208982A (en) * | 2021-12-31 | 2022-03-22 | 中国海洋大学 | Anti-inflammatory, growth-promoting and immunity-promoting turbot feed and preparation method thereof |
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| CN106036197A (en) * | 2016-05-25 | 2016-10-26 | 中国水产科学研究院黑龙江水产研究所 | Fish-meal-free sturgeon feed additive, preparation method and use method thereof |
| CN109275800A (en) * | 2017-07-20 | 2019-01-29 | 无锡三智生物科技有限公司 | It is a kind of for protecting the feed addictive of aquatic livestock intestinal health |
| CN108013213A (en) * | 2018-02-07 | 2018-05-11 | 高嘉泰 | A kind of moist microorganism particle fermented feed for aquatic livestock and preparation method thereof |
| CN110384111A (en) * | 2019-07-18 | 2019-10-29 | 无锡中顺生物技术有限公司 | A kind of synergist of lactic acid bacteria and its application |
| CN114208982A (en) * | 2021-12-31 | 2022-03-22 | 中国海洋大学 | Anti-inflammatory, growth-promoting and immunity-promoting turbot feed and preparation method thereof |
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