CN108013213A - A kind of moist microorganism particle fermented feed for aquatic livestock and preparation method thereof - Google Patents
A kind of moist microorganism particle fermented feed for aquatic livestock and preparation method thereof Download PDFInfo
- Publication number
- CN108013213A CN108013213A CN201810122641.2A CN201810122641A CN108013213A CN 108013213 A CN108013213 A CN 108013213A CN 201810122641 A CN201810122641 A CN 201810122641A CN 108013213 A CN108013213 A CN 108013213A
- Authority
- CN
- China
- Prior art keywords
- feed
- fermented feed
- bacterium solution
- fermentation
- aquatic livestock
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002245 particle Substances 0.000 title claims abstract description 51
- 244000144972 livestock Species 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 34
- 244000005700 microbiome Species 0.000 title claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 85
- 241000894006 Bacteria Species 0.000 claims abstract description 75
- 238000000855 fermentation Methods 0.000 claims abstract description 63
- 230000004151 fermentation Effects 0.000 claims abstract description 61
- 238000011282 treatment Methods 0.000 claims abstract description 18
- 238000005469 granulation Methods 0.000 claims abstract description 14
- 230000003179 granulation Effects 0.000 claims abstract description 14
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 9
- 238000004806 packaging method and process Methods 0.000 claims abstract description 8
- 238000005453 pelletization Methods 0.000 claims abstract description 8
- 150000001413 amino acids Chemical class 0.000 claims abstract description 4
- 238000002203 pretreatment Methods 0.000 claims abstract description 4
- 229940088594 vitamin Drugs 0.000 claims abstract description 4
- 239000011782 vitamin Substances 0.000 claims abstract description 4
- 235000013343 vitamin Nutrition 0.000 claims abstract description 4
- 229930003231 vitamin Natural products 0.000 claims abstract description 4
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 4
- 239000004615 ingredient Substances 0.000 claims description 66
- 239000000463 material Substances 0.000 claims description 58
- 235000012054 meals Nutrition 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 26
- 102000004169 proteins and genes Human genes 0.000 claims description 26
- 108090000623 proteins and genes Proteins 0.000 claims description 26
- 238000002156 mixing Methods 0.000 claims description 21
- 238000011081 inoculation Methods 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 16
- 239000002994 raw material Substances 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 15
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 14
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 14
- 235000013312 flour Nutrition 0.000 claims description 14
- 239000000306 component Substances 0.000 claims description 13
- 108010011619 6-Phytase Proteins 0.000 claims description 12
- 229940085127 phytase Drugs 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 10
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 9
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 9
- 239000011230 binding agent Substances 0.000 claims description 9
- 239000002054 inoculum Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 239000012533 medium component Substances 0.000 claims description 9
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 9
- 240000007594 Oryza sativa Species 0.000 claims description 8
- 235000007164 Oryza sativa Nutrition 0.000 claims description 8
- 108091005804 Peptidases Proteins 0.000 claims description 8
- 239000004365 Protease Substances 0.000 claims description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 8
- 229940041514 candida albicans extract Drugs 0.000 claims description 8
- 210000003746 feather Anatomy 0.000 claims description 8
- 235000013305 food Nutrition 0.000 claims description 8
- 235000013379 molasses Nutrition 0.000 claims description 8
- 239000008188 pellet Substances 0.000 claims description 8
- 235000009566 rice Nutrition 0.000 claims description 8
- 239000012138 yeast extract Substances 0.000 claims description 8
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 6
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 6
- 244000105624 Arachis hypogaea Species 0.000 claims description 6
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 6
- 235000018262 Arachis monticola Nutrition 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 6
- 241000239366 Euphausiacea Species 0.000 claims description 6
- 241000287828 Gallus gallus Species 0.000 claims description 6
- 239000004366 Glucose oxidase Substances 0.000 claims description 6
- 108010015776 Glucose oxidase Proteins 0.000 claims description 6
- 241000238371 Sepiidae Species 0.000 claims description 6
- 229910021536 Zeolite Inorganic materials 0.000 claims description 6
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 6
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 claims description 6
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 6
- 229940106157 cellulase Drugs 0.000 claims description 6
- 239000006071 cream Substances 0.000 claims description 6
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 6
- 229940116332 glucose oxidase Drugs 0.000 claims description 6
- 235000019420 glucose oxidase Nutrition 0.000 claims description 6
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 235000020232 peanut Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 6
- 229920000053 polysorbate 80 Polymers 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 6
- 235000010413 sodium alginate Nutrition 0.000 claims description 6
- 229940005550 sodium alginate Drugs 0.000 claims description 6
- 239000000661 sodium alginate Substances 0.000 claims description 6
- 239000010457 zeolite Substances 0.000 claims description 6
- 239000005996 Blood meal Substances 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- 238000000227 grinding Methods 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 4
- 230000008961 swelling Effects 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 230000033228 biological regulation Effects 0.000 claims description 3
- 238000007906 compression Methods 0.000 claims description 3
- 230000006835 compression Effects 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- 239000000945 filler Substances 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 3
- 239000002002 slurry Substances 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 3
- 239000001393 triammonium citrate Substances 0.000 claims description 3
- 235000011046 triammonium citrate Nutrition 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 2
- 210000000481 breast Anatomy 0.000 claims description 2
- 239000000796 flavoring agent Substances 0.000 claims description 2
- 235000019634 flavors Nutrition 0.000 claims description 2
- 102000039446 nucleic acids Human genes 0.000 claims description 2
- 108020004707 nucleic acids Proteins 0.000 claims description 2
- 150000007523 nucleic acids Chemical class 0.000 claims description 2
- 239000002773 nucleotide Substances 0.000 claims description 2
- 125000003729 nucleotide group Chemical group 0.000 claims description 2
- 229920001184 polypeptide Polymers 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 229910001220 stainless steel Inorganic materials 0.000 claims description 2
- 239000010935 stainless steel Substances 0.000 claims description 2
- 102000040350 B family Human genes 0.000 claims 1
- 108091072128 B family Proteins 0.000 claims 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims 1
- 235000012907 honey Nutrition 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 18
- 230000001007 puffing effect Effects 0.000 abstract description 10
- 239000012530 fluid Substances 0.000 abstract description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 4
- 150000003384 small molecules Chemical class 0.000 abstract description 4
- 108010062877 Bacteriocins Proteins 0.000 abstract description 3
- 150000001720 carbohydrates Chemical class 0.000 abstract description 3
- 229920002521 macromolecule Polymers 0.000 abstract description 3
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 3
- 239000005416 organic matter Substances 0.000 abstract description 3
- 238000010564 aerobic fermentation Methods 0.000 abstract description 2
- 238000000354 decomposition reaction Methods 0.000 abstract description 2
- 238000007654 immersion Methods 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 abstract description 2
- 239000010828 animal waste Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 49
- 235000018102 proteins Nutrition 0.000 description 24
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 21
- 230000000052 comparative effect Effects 0.000 description 19
- 238000012360 testing method Methods 0.000 description 19
- 241000238557 Decapoda Species 0.000 description 18
- 241001465754 Metazoa Species 0.000 description 17
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 15
- 239000011574 phosphorus Substances 0.000 description 15
- 229910052698 phosphorus Inorganic materials 0.000 description 15
- 239000000047 product Substances 0.000 description 14
- 230000000694 effects Effects 0.000 description 13
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 12
- 102000004190 Enzymes Human genes 0.000 description 11
- 108090000790 Enzymes Proteins 0.000 description 11
- 229940088598 enzyme Drugs 0.000 description 11
- 238000009360 aquaculture Methods 0.000 description 10
- 230000008569 process Effects 0.000 description 10
- 244000144974 aquaculture Species 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 241000192700 Cyanobacteria Species 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 239000004310 lactic acid Substances 0.000 description 6
- 235000014655 lactic acid Nutrition 0.000 description 6
- 235000019779 Rapeseed Meal Nutrition 0.000 description 5
- 229920002472 Starch Polymers 0.000 description 5
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 210000004185 liver Anatomy 0.000 description 5
- 238000010298 pulverizing process Methods 0.000 description 5
- 239000004456 rapeseed meal Substances 0.000 description 5
- 235000019698 starch Nutrition 0.000 description 5
- 239000008107 starch Substances 0.000 description 5
- 241000927735 Penaeus Species 0.000 description 4
- 238000013329 compounding Methods 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 230000007062 hydrolysis Effects 0.000 description 4
- 238000006460 hydrolysis reaction Methods 0.000 description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 description 4
- 239000011707 mineral Substances 0.000 description 4
- 235000010755 mineral Nutrition 0.000 description 4
- 235000019624 protein content Nutrition 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 3
- 230000000433 anti-nutritional effect Effects 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 210000000936 intestine Anatomy 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000012086 standard solution Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- 241000195493 Cryptophyta Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000000205 computational method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000019621 digestibility Nutrition 0.000 description 2
- 238000012851 eutrophication Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 235000015099 wheat brans Nutrition 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 229920000856 Amylose Polymers 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000238553 Litopenaeus vannamei Species 0.000 description 1
- 229920000881 Modified starch Polymers 0.000 description 1
- 239000004368 Modified starch Substances 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000037237 body shape Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000019426 modified starch Nutrition 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 235000020265 peanut milk Nutrition 0.000 description 1
- 238000011417 postcuring Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000011265 semifinished product Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/24—Animal feeding-stuffs from material of animal origin from blood
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Marine Sciences & Fisheries (AREA)
- Biochemistry (AREA)
- Inorganic Chemistry (AREA)
- Insects & Arthropods (AREA)
- Sustainable Development (AREA)
- Birds (AREA)
- Botany (AREA)
- Mycology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of moist microorganism particle fermented feed for aquatic livestock and preparation method thereof, by improved feed formula, pre-treatment, expanding treatment, pelletization treatment, fermentation process, packaging are prepared, and have the following advantages：(1) soft, elastic good, in good taste, input water body rear stability is strong, not defeated and dispersed；Increase is by feeding probability；(2) contain a large amount of beneficial microbes, macromolecular substances in feedstuff are decomposed into the amino acid of small molecule, carbohydrate, active peptide, vitamin, bacteriocin etc., promote to digest and assimilate；(3) discharge of nitrogen in animal wastes is reduced, promotes the decomposition of substrate organic matter, keeps the environment of purification of water quality；(4) granulation after feed is first puffing, more accurate combination between each component, then aerobic and anaerobic fermentation is carried out in fermentation bag after adding zymotic fluid immersion, allows beneficial bacterium that the macromolecular substances in feedstuff are decomposed into the amino acid of small molecule, carbohydrate, active peptide, vitamin, bacteriocin etc..
Description
Technical field
The present invention relates to fermentative feedstuff of microbe field, more particularly to a kind of moist microorganism particle for aquatic livestock
Fermented feed and preparation method thereof.
Background technology
Recently as the development of microorganism fermentation process, fermentative feedstuff of microbe causes aquaculture industry widely emerging
Interest, fermentative feedstuff of microbe refer under manual control condition, by the metabolic activity of microorganism, by the macromolecular complex in feed
Matter and anti-nutritional factors are decomposed or conversion, and generation is more advantageous to feed intake and what is utilized is rich in high-activity probiotics and its metabolism
The feed or raw material of product.Fermentative feedstuff of microbe has natural fermenting aroma, contains substantial amounts of beneficial bacterium.Fermented product
PH value is relatively low, 4.5 or so, contains more lactic acid and acetic acid.It is this at the same have acidulant, probiotics characteristic and
The fermentation material of similar antibiotic growth factor function, has nontoxic, noresidue, free of contamination advantage.
Fermentative feedstuff of microbe has its own unique superiority compared with conventional feed；1st, using cheap agricultural and
The forage protein raw material of light industry by-product production high quality, 2, obtain high activity beneficial microbe.3rd, new nonreactive fermentation is raised
Material solve well promote growth of animal and noresidue, it is pollution-free between contradiction.4th, current health environment-friendly type fishery is met
The requirement of development.
In the market major part fermented feed is all that agricultural or the single raw material of light industry byproduct or several raw materials are carried out
Fermented after simple compounding using anaerobism or aerobic solid-fermented technique, dry and be applied in feed after Partial fermentation
Go, for example fermented bean dregs, fermentation cotton dregs etc..Portioned product ferments after simple compounding, is applied to scene, for example：Albumen
Type fermentation material is mainly using dregs of beans as primary raw material+wheat bran+glucose+strain；The fermentation material of carbon source type mainly using wheat bran as
Primary raw material+a small amount of protein raw materials+glucose+strain.
And the fermented feed used in aquaculture at present is typically to use crop by-product as raw material, crush as powdery
Simple compounding, which carries out fermentation, afterwards becomes aquatic products powdery fermentation material, it, which feeds mode, two kinds：One kind by being stirred in powder,
VAS application -to-terminal service farm.And the most of puffing or grit not waited from 0.3mm to 3.5mm of all searching for food of aquiculture animal is raised
Material, so powdery fermented feed is difficult by aquiculture animal feeding to enteron aisle.It is to send out powdery that another, which feeds mode,
Ferment material and conventional granulates feed, which are bound together to put into water body, allows aquatic livestock to search for food, and mash feed is difficult and often
Rule pellet effectively bonds, and after especially putting into water body, powdery fermentation material scatter with conventional granulates feed quickly, aquatic livestock
The fermentation material searched for food into intestines and stomach is very limited, it is impossible to which playing fermented feed just really improves enteron aisle, improves feed digestion
The effect of absorptivity.The powdery fermented feed actually added in aquaculture process, is mostly just intended only as a tune
Water, change aquatic products to adjust Aquacultural water, is not eaten by aquatic livestocks such as fish, shrimp, crabs really, improves intestines
Road, reaches the inside and outside effect taken into account；The burden of bigger is caused to breeding water body on the contrary, so that the water ring to the aquaculture later stage
Cause ammonia nitrogen, content of nitrite high in border.Allow water quality adjustment to be operated in the cultivation later stage to be difficult to, so most of cultivation at present
Person have realized that powdery fermented feed there are the drawbacks of, simply a small amount of use cultivation early period some.And in the middle and later periods
For regulating water quality in breeding process.And most culturist can use single or compounding beneficial bacterium such as：Withered grass gemma
Bacillus, streptococcus fecalis etc..And do not have to substantially for powdery fermented feed, which forms fermented feed in aquaculture process
The difficult situation that can not be used by large area.
Also it is that bulky grain is sent out to have some producers or cultivation company to pass through simple coarse crushing with feedstuff such as corn, soya bean etc.
Water body is put into after ferment；This ferment particulate feed is for aquatic livestock, and nutrition is single, and particle size is inconsistent, after immersion
Poor water resistance, is not also prepared, feeding effect is poor according to the nutritional need of aquatic livestock and feeding custom.
How to prepare it is a kind of from formulation, feed formula and prepare process on be combined together, develop one
Kind ingests suitable for aquatic livestock, is full of nutrition, promotes the moist microorganism particle fermentation feeding value digested and assimilated to the full extent
It must study.
The content of the invention
Goal of the invention：In order to overcome defect existing in the prior art, the present invention to be protected after proposing a kind of input water body
Shape invariance in the long period is held, holding time length, is easily searched for food in time by aquiculture animal, is conducive to enteron aisle maintenance, increases
Added with bacteria group, promote to digest and assimilate, moist microorganism particle fermented feed of purpose of healthy aquaculture and preparation method thereof.
Technical solution：In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：One kind is moved for aquatic products
The moist microorganism particle fermented feed of thing, is specially graininess, its particle diameter is 0.3mm-6.0mm, calculated by mass including 4-7
The feed ingredient one, 3-6 parts of feed ingredient two and 0.02-0.05 parts of feed ingredient three of part；
Each material quality percentage is as follows in wherein described feed ingredient one：Peanut meal 20%-40%, dregs of beans 30%-
50%, rapeseed meal 5%-10%, chicken meal 0-7%, blood meal 2%-6%, hydrolyzed feather meal 1%-3%, rice bran 6%-10%；
Each material quality percentage is as follows in the feed ingredient two：Krill meal 2%-6%, yeast extract 2%-6%,
Cuttlefish cream 6%-10%, Strong flour 50%-80%, calcium dihydrogen phosphate 1%-2%, binding agent 0.1%-1%, salt 0.5%-
1%, zeolite powder 5%-14%；
Each material quality percentage is as follows in the feed ingredient three：Glucose oxidase 10%-50%, DP100 albumen
Enzyme 30%-60%, cellulase 20%-50%, phytase 20%-40%.
The Strong flour ratio calculated by mass for accounting for the fermented feed gross mass is more than 30%.Strong flour passes through
Expanding treatment improves the gelatinization degree of starch, improves the water resistance of the feed after follow-up granulation.
It is more highly preferred to, the binding agent is specially sodium alginate.
For the yeast extract using food yeast as raw material, the technique using self-dissolving, enzymolysis, separation, concentration is thin by yeast
The protein of intracellular, nucleic acid etc. are refined after being degraded and formed；Main component is polypeptide, amino acid, flavour nucleotide, B races dimension
Raw element and trace element；It is a kind of excellent natural flavouring, and ideal biological culture medium raw material and fermentation industry
In primary raw material, its effect is suitable with 8 times of yeast, can greatly improve the throughput rate and fermented product yield of strain.
It is more highly preferred to, the moisture of the fermented feed is 25%-45%.
The invention also discloses a kind of preparation method of above-mentioned moist microorganism particle fermented feed for aquatic livestock,
It is characterized by comprising following steps：
1) weigh：The preparing raw material in clean each fermented feed component is weighed by formula regulation number；
2) pre-treatment：
2-A) mix：Three kinds of fermented feed components are carried out to separate mixed processing；
Wherein feed ingredient one is mixed at room temperature using single shaft paddle formula mixer, and mixability is the change of material
Different coefficient is less than 5%；It is then sent to water droplet formula pulverizer to be crushed, powder particle diameter is spare for 50-60 mesh；
Feed ingredient two carries out mixing for standby use using horizontal-type biaxial paddle formula mixer；
Feed ingredient three carries out mixing for standby use using drum mixer；
2-B) ultramicro grinding：The feed ingredient one after crushing is weighed in proportion and feed ingredient two puts into horizontal-type biaxial paddle
Formula mixer is mixed at room temperature；Ultra micro filler bin is put into after mixing to be crushed with vertical shaft type micronizer, is crushed
Granularity reaches more than 100 mesh, obtains the mixed material A being made of feed ingredient one and feed ingredient two；
3) expanding treatment：The mixed material A input expanding treatment equipment that step 2) is obtained carries out expanding treatment：Using wet
Method plavini, it is 125-138 DEG C to control swelling temperature, and pressure is 34-37 atmospheric pressure, and moisture is controlled in 20%-25%；
4) pelletization treatment：Mixed material A after step 3) expanding treatment is pelletized in bulking equipment；Temperature 100-
140 DEG C, compression ratio 20-28, screw speed 500-800rpm, moisture 22%-26%；Pellet after granulation is as cold as
It is spare after 25-35 DEG C；
5) fermentation process：
5-A) the preparation of inoculation fermentation bacterium solution：Three kinds of EM bacterium, candida utili, lactobacillus plantarum zymophytes are prepared respectively
Liquid；According to the ratio of bacterium solution volume；Wherein by volume by the EM bacterium solutions of 30%-80%, the candida utili of 10%-30%
Bacterium solution；The lactobacillus plantarum bacterium solution of 10%-60% is uniformly mixed to obtain mixed fermentation bacterium solution in fermentation tank；By mixing in step 2)
Spare feed ingredient three is added in mixed fermentation bacterium solution according to required ratio, is uniformly mixing to obtain inoculation fermentation bacterium solution；
5-B) combined inoculation：The spare mixed material A of step 4) granulation postcooling is put into plough mixer, step 5-
A inoculation fermentation bacterium solution made from) is according to the ratio spray inoculation of the 5%-15% of mixed material A mass into plough mixer
On mixed material A finished feed；
6) pack：Moist particle is packed using one-way ventilating valve packaging bag, and the moisture of the feed after granulation exists
Between 28%-45%, packaging, which is placed in 28-32 DEG C of ferment at constant temperature workshop, ferments three days to obtain the final product.
In order to obtain optimal ferment effect, step 5-A) in the medium component and in mass prepared of EM bacterium bacterium solution
The proportioning of calculation is：Peptone 1%, molasses 10%, potassium dihydrogen phosphate 0.05%, water surplus；PH is 7.0；EM strain inoculum concentrations are
The 5% of culture medium gross mass.
Further, step 5-A) in the medium component and calculated by mass prepared of candida utili bacterium solution
Match and be：Corn pulp 0.5%, molasses 0.5%, glucose 2.0%, KH2P040.1%th, (NH4)2SO41%th, water surplus；PH is
6.0-6.5；Candida utili strain inoculum concentration is the 2% of culture medium gross mass.
Further, step 5-A) in lactobacillus plantarum bacterium solution prepare medium component and proportioning be：Beef extract
0.6%th, molasses 0.8%, potassium dihydrogen phosphate 0.2%, Triammonium citrate 0.2%, Tween 80 0.1%, water surplus, pH 6.1-
6.3；Lactobacillus plantarum strain inoculum concentration be culture medium gross mass 2%, wherein except Tween 80 percentage in terms of volume, its
His percentage of component is calculated by mass.
It is more highly preferred to, facility for granulating described in step 4) uses stainless steel ring die pelletizing equipment, and pressure roller is serration；Motor
Power main motor is six grades.
Beneficial effect：A kind of moist microorganism particle fermented feed provided by the invention and preparation method thereof, has following
Advantage：
(1) contain phytase in feed formula, reduce the additive amount of phosphorus in feed, effectively prevent due to feeding
It is surplus, the too high levels of organic matter and phosphorus in water body are caused, cause body eutrophication, cause blue algae bloom.
(2) DP100 protease is contained in feed formula, DP100 protease is a kind of hydrolyzed feather meal of Nuo Weisi companies
Protease, can be good at the anti-nutritional factors in hydrolyzed feather meal and efficient degradation dregs of beans, improve protein utilization rate, can
The usage amount of animal protein fish meal in aquatic feeds is reduced, so as to reduce feed cost, in addition improves aquatic livestock to feed
Middle protein is digested and assimilated, the nitrogen source being discharged into water body it is opposite reduce many, reduce feeding pair due to feed
Pollution caused by water quality；
(3) finished feed is soft, elasticity is suitable, and moisture is suitable, containing substantial amounts of beneficial microbe, after putting into water body
Interior shape invariance, not defeated and dispersed can be maintained for a long time in water body；And mouthfeel is suitable, there is more palatability and food calling
Property, the characteristic of aquaculture conventional feed is not only maintained, but also solve the moisture for moist particulate material of fermenting, micro- life well
The problem of thing activity and storage life.
(4) graininess increases probability of a large amount of beneficial bacteriums by aquatic livestock feeding into intestines and stomach, and its distinctive is matched somebody with somebody
Side further increases digestibility of the aquatic livestock to feed, reduces the discharge of nitrogen in excrement；And containing big in excrement
The beneficial bacterium of amount, promotes the decomposition of substrate organic matter；Keep the environment of purification of water quality；Benign promotion growth of animal and nothing are reached
Residual, it is pollution-free between two-win.
(5) feed is subjected to puffing rear granulation, makes accurate combination between each component, then added after zymotic fluid soaks and sending out
Aerobic and anaerobic fermentation is carried out in ferment bag, allows beneficial bacterium that the macromolecular substances in feedstuff are decomposed into the amino of small molecule
Acid, carbohydrate, active peptide, vitamin, bacteriocin etc..
(6) the soft particle of microbial fermentation wet type containing active microorganism is made in pellet by fermentation technique again after pelletizing
Feed, ensures that the gas that the strain in fermentation material produces in storage can be arranged in time with one-way ventilating valve bag packaging
Remove, increase the preservation service life of feed；And solve conventional feed kills beneficial microbe and enzyme during high temperature granulating
The problem of preparation isoreactivity composition.
Embodiment
With reference to embodiment, the present invention is described in further detail：
Embodiment 1：
A kind of moist microorganism particle fermented feed for aquatic livestock, is specially graininess, its particle diameter is 0.3mm-
6.0mm, moisture 30%；
The feed ingredient two and 0.02 part of feed ingredient calculated by mass for including 7 parts of one, 3.08 part of feed ingredient
Three；
Each material quality percentage is as follows in wherein described feed ingredient one：Peanut meal 22%, dregs of beans 50%, rapeseed meal
10%, chicken meal 7%, hydrolyzed feather meal 1%, rice bran 10%；With specific reference to different aquatic livestocks for protein and energy
Demand it is different, prepared；
Each material quality percentage is as follows in the feed ingredient two：Krill meal 6%, cuttlefish cream 6%, yeast extract
2%, Strong flour 72%, calcium dihydrogen phosphate 2%, binding agent sodium alginate 1%, salt 1%, zeolite powder 10%；Predominantly water
Produce animal and required mineral matter and the feedstuff with phagostimulating effect are provided, the addition of Strong flour and binding agent ensure that hair
The water resistance of ferment feed in water；
Each material quality percentage is as follows in the feed ingredient three：Glucose oxidase 20%, DP100 protease
30%th, cellulase 20%, phytase 30%；The addition of rational enzyme preparation can improve digestion of the feed in enteron aisle and inhale
Receive, improve efficiency of feed utilization.Phytase is added in feed formula, reduces the adding proportion of phosphorus in feed, so as to reduce indirectly
The content of phosphorus in water body, due to the excessive outburst cyanobacteria of phosphorus content in pre- waterproof body.
Embodiment 2：
A kind of moist microorganism particle fermented feed for aquatic livestock, is specially graininess, its particle diameter is 0.3mm-
6.0mm, moisture 35%；
The feed ingredient two and 0.05 part of feed ingredient calculated by mass for including 6 parts of one, 3.95 part of feed ingredient
Three；
Each material quality percentage is as follows in wherein described feed ingredient one：Peanut meal 30%, dregs of beans 40%, rapeseed meal
10%, chicken meal 5%, blood meal 6%, hydrolyzed feather meal 3%, rice bran 6%；With specific reference to different aquatic livestocks for protein
It is different with the demand of energy, prepared；
Each material quality percentage is as follows in the feed ingredient two：Krill meal 6%, yeast extract 2%, cuttlefish cream
10%, Strong flour 70%, calcium dihydrogen phosphate 2%, binding agent sodium alginate 1%, salt 1%, zeolite powder 8%；
Predominantly aquatic livestock provides required mineral matter and the feedstuff with phagostimulating effect, Strong flour and bonding
The addition of agent ensure that the water resistance of fermented feed in water；
Each material quality percentage is as follows in the feed ingredient three：Glucose oxidase 20%, DP100 protease
40%th, cellulase 20%, phytase 20%.The addition of rational enzyme preparation can improve digestion of the feed in enteron aisle and inhale
Receive, improve efficiency of feed utilization.Phytase is added in feed formula, reduces the adding proportion of phosphorus in feed, so as to reduce indirectly
The content of phosphorus in water body, due to the excessive outburst cyanobacteria of phosphorus content in pre- waterproof body.
Embodiment 3：
A kind of moist microorganism particle fermented feed for aquatic livestock, is specially graininess, its particle diameter is 0.3mm-
6.0mm, moisture 42%；
The feed ingredient two and 0.05 part of feed ingredient calculated by mass for including 7 parts of one, 3.95 part of feed ingredient
Three；
Each material quality percentage is as follows in wherein described feed ingredient one：Peanut meal 30%, dregs of beans 45%, rapeseed meal 5%,
Chicken meal 7%, blood meal 6%, hydrolyzed feather meal 1%, rice bran 6%；With specific reference to different aquatic livestocks for protein and energy
The demand of amount is different, is prepared；
Each material quality percentage is as follows in the feed ingredient two：Krill meal 6%, yeast extract 2%, cuttlefish cream
8%, Strong flour 72%, calcium dihydrogen phosphate 2%, binding agent sodium alginate 1%, salt 1%, zeolite powder 8%；Predominantly aquatic products
Animal provides required mineral matter and the feedstuff with phagostimulating effect, and the addition of Strong flour ensure that fermented feed in water
In water resistance；
The following glucose oxidase 20% of each material quality percentage in the feed ingredient three, DP100 protease 50%,
Cellulase 10%, phytase 20%；The addition of rational enzyme preparation can improve feed digesting and assimilating in enteron aisle, improve
Efficiency of feed utilization.Phytase is added in feed formula, reduces the adding proportion of phosphorus in feed, so as to reduce indirectly in water body
The content of phosphorus, due to the excessive outburst cyanobacteria of phosphorus content in pre- waterproof body.
Embodiment 4：
A kind of moist microorganism particle fermented feed for aquatic livestock, is specially graininess, its particle diameter is 0.3mm-
6.0mm, moisture 38%；
The feed ingredient two and 0.02 part of feed ingredient calculated by mass for including 6 parts of one, 3.98 part of feed ingredient
Three；
Each material quality percentage is as follows in wherein described feed ingredient one：Peanut meal 30%, dregs of beans 45%, rapeseed meal 5%,
Chicken meal 5%, blood meal 2%, hydrolyzed feather meal 3%, rice bran 10%；With specific reference to different aquatic livestocks for protein and energy
The demand of amount is different, is prepared；
Each material quality percentage is as follows in the feed ingredient two：Krill meal 6%, yeast extract 6%, cuttlefish cream
8%, Strong flour 68%, calcium dihydrogen phosphate 2%, binding agent sodium alginate 1%, salt 1%, zeolite powder 8%；Predominantly aquatic products
Animal provides required mineral matter and the feedstuff with phagostimulating effect, and the addition of Strong flour ensure that fermented feed in water
In water resistance；
Each material quality percentage is as follows in the feed ingredient three：Glucose oxidase 10%, DP100 protease
50%th, cellulase 20%, phytase 20%；The addition of rational enzyme preparation can improve digestion of the feed in enteron aisle and inhale
Receive, improve efficiency of feed utilization.Phytase is added in feed formula, reduces the adding proportion of phosphorus in feed, so as to reduce indirectly
The content of phosphorus in water body, due to the excessive outburst cyanobacteria of phosphorus content in pre- waterproof body.
Embodiment 5：
Using the formula of above-described embodiment 1 as preparing raw material, a kind of moist microorganism particle for aquatic livestock, which ferments, raises
The preparation method of material, includes the following steps：
1) weigh：The preparing raw material in clean each fermented feed component is weighed by formula regulation number；
2) pre-treatment：
2-A) mix：Three kinds of fermented feed components are carried out to separate mixed processing；
Wherein feed ingredient one is mixed at room temperature using single shaft paddle formula mixer, in preceding processing, to feed
The mixture homogeneity of component one is of less demanding；It is required that mixability is less than 5% for the coefficient of variation of material；Then conveying auger is used
It is delivered to water droplet formula pulverizer to be crushed, powder particle diameter is spare for 50 mesh；
Wherein the pulverizing chamber of water droplet formula pulverizer is changed to droplet-shaped by the circle of common hammer crusher, can so increase
The effective area of pulverizing chamber sieve plate, and material can be destroyed and form circulation in pulverizing chamber, be conducive to crushing rear material discharge pulverizing chamber；
In addition, the pulverizing chamber of droplet-shaped pulverizer crushes design using two-hit, while position of the hammer leaf on rotor, energy can be changed
The crushing of thick, thin, fine three kinds of formation is formed, the more common hammer crusher of yield is crushed and improves more than 15%, powder particle diameter reaches
To 50 mesh, the requirement to the primary comminution fineness of feed ingredient one is adapted to；
Feed ingredient two carries out mixing for standby use using horizontal-type biaxial paddle formula mixer；
The mixing of feed ingredient two is pre-mixed core small powder primarily directed to using Strong flour as carrier, because core
Additive amount of the heart small powder in feed is relatively small, it is necessary to ensures that it is uniformly distributed in feed, it is pre-mixed
After be then added in feed, so feed ingredient two must use mixture homogeneity higher horizontal-type biaxial paddle formula mix
Machine is mixed, and horizontal-type biaxial paddle formula mixer is a kind of efficiently short-period mixer, opposite by two direction of rotation
Rotor forms, and the paddle of multiple and different angles is welded with rotor, and on the one hand paddle drives material to be rotated along inner wall, on the one hand drive
Material or so stirs, overlapping in the infall of two rotors, forms a weightless area, in this region, no matter the shape of material,
How are size and density, can float, in instantaneous weight loss state, so that material shape is stirred into comprehensive continuous circulation, mutually
Staggeredly shear, so as to reach quick soft and uniformly mixed effect；
Feed ingredient three carries out mixing for standby use using drum mixer；
The component of feed composition three is mainly enzyme preparation, its mixture homogeneity is more demanding, it is necessary to first in drum mixer
It is added again after middle mixing.
2-B) ultramicro grinding：The feed ingredient one after crushing is weighed in proportion and feed ingredient two puts into horizontal-type biaxial paddle
Formula mixer is mixed at room temperature；Ultra micro filler bin is put into after mixing to be crushed with vertical shaft type micronizer, is crushed
Granularity reaches more than 100 mesh, obtains the mixed material A being made of feed ingredient one and feed ingredient two；
3) expanding treatment：The mixed material A that step 2) is obtained puts into expanding treatment equipment double-screw type extrusion expansion machine
Carry out expanding treatment：Using wet expansion method, it is 125-138 DEG C to control swelling temperature, and pressure is 34-37 atmospheric pressure, moisture
Control is in 20%-25%；Moisture in Feed after puffing is due to moisture evaporation, and moisture 16%-20%, temperature is in 75-85
DEG C, be cooled to 35-40 DEG C it is spare；
Feed ingredient one and feed ingredient two are puffing after mixing mainly to be combined together protein and starch matrix, raises
Not easily runed off when feeding, just protein release is come out only when the digested enzymically decomposed starch of animal body, improves protein
Potency.In addition, puffing process is also denatured protein, many anti-nutritional factors are passivated, while change protein
Tertiary structure, shortens hydrolysis time of the protein in enteron aisle, aquatic animal can be made to be more easy to digest and assimilate, and improves feed and disappears
Change absorptivity.The content of starch in the present invention reaches more than the 30% of whole feed formula at the same time, good by Swelling Functions
The gelatinization degree of starch is improved, allows amylose to generate modified starch, there is very strong water imbibition and binding function, high water suction
Property can to add the up to zymocyte liquid of as many as 30%-45% into product in subsequent technique, strong binding function, is improved
Water resistance of the fermented feed in water body.
4) pelletization treatment：Mixed material A after step 3) expanding treatment is pelletized in bulking equipment；Temperature 100-
140 DEG C, compression ratio 20-28, screw speed 500-800rpm, moisture 22%-26%；Pellet after granulation is as cold as
It is spare after 25-35 DEG C；
5) fermentation process：
5-A) the preparation of inoculation fermentation bacterium solution：Three kinds of EM bacterium, candida utili, lactobacillus plantarum zymophytes are prepared respectively
Liquid；Three kinds of strain seeds are purchased from the green world bio tech ltd in Hubei；According to the ratio of bacterium solution volume；Wherein with body
Product is counted 80% EM bacterium solutions, 10% candida utili bacterium solution；10% lactobacillus plantarum bacterium solution mixes in fermentation tank
It is uniform to obtain mixed fermentation bacterium solution；The feed ingredient three of mixing for standby use in step 2) is added into mixed fermentation bacterium solution according to required ratio
In, it is uniformly mixing to obtain inoculation fermentation bacterium solution；
Conventional aquatic feeds work in-process, which will generally pass through, strengthens the techniques such as quenched, granulation (or puffing), post curing, and
At present, mainly there are Bacillus acidi lactici, streptococcus, yeast, bud pole bacterium etc. using more microorganism formulation in feed, this is slightly raw
Thing preparation is particularly sensitive to temperature, and when puffing pelleting temperature is more than 85 DEG C, it is active by total loss；The optimum temperature of enzyme exists
35-40 DEG C, but the temperature in puffing pelletization reaches 120-150 DEG C, and (cause moisture higher in feed to be lived with high humidity
Degree), high pressure (change zymoprotein Spatial Multi-Dimensional structure and be denatured), in such a situa-tion, most of enzyme preparations activity all
It will lose totally；So the technique that the present invention adds after using is these heat sensitive components i.e. the enzyme of three kinds of feed constituent
Preparation, is proportionally added in bacterium solution after mixing, and two mixture puffings are formed in feedstuff composition one and feedstuff
Spray into again afterwards wherein.
5-B) combined inoculation：The spare mixed material A of step 4) granulation postcooling is put into plough mixer, step 5-
A inoculation fermentation bacterium solution made from) is sprayed according to the ratio of the 5%-15% of mixed material A mass by metering pump and liquid-jet device
Mist is seeded on the mixed material A in plough mixer to obtain feed semi-finished product；The operation principle of wherein plough mixer is suitable for
The mixing of viscosity or gluey additive, it is optimal selection to mix for solid-liquid and make moist particle；
Wherein, step 5-A) in EM liquid making methods it is as follows：Medium component and proportioning calculated by mass are：Albumen
Peptone 1%, molasses 10%, potassium dihydrogen phosphate 0.05%, water surplus；It will adjust and train in the fermentation tank of culture medium input 100L in proportion
The pH value for supporting base is 7.0 or so；It is passed through steam and maintains the temperature at 121 DEG C of sterilization 20min, is then cooled to 30-35 DEG C and connects
Enter EM strains, inoculum concentration 5%, keeps constant temperature to be cultivated；Zymotic fluid is represented between reaching 3.8-4.0 using pH meter detection Ph
Fermentation stability, obtains EM fermented liquids.
Candida utili bacterium solution preparation method is as follows：The medium component of preparation and proportioning calculated by mass are：It is beautiful
Rice ＆ peanut milk 0.5%, molasses 0.5%, glucose 2.0%, KH2P040.1%th, (NH4)2SO41%th, water surplus；In proportion will culture
In the fermentation tank of base input 100L, adjusting pH value is 6.0-6.5；It is passed through steam and maintains the temperature at 121 DEG C of sterilization 20min,
30 DEG C of access Bacillus subtilis strains are cooled to, inoculum concentration 2% keeps constant temperature to carry out aerobic culture, and blender initially turns
Speed is 200rpm/min.If detection pH value reaches 3.6-3.8 after culture 18h, spectrophotometer is used after bacterium solution is diluted 26 times
When the OD values of detection bacterium solution reach 0.50 ± 0.01, represent zymotic fluid fermentation stability, obtain the zymotic fluid of candida utili.
Lactobacillus plantarum bacterium solution preparation method is as follows：Medium component and proportioning are：Beef extract 0.6%, molasses 0.8%,
Potassium dihydrogen phosphate 0.2%, Triammonium citrate 0.2%, Tween 80 0.1%, water surplus；Wherein except the percentage of Tween 80 is with body
Product calculates, and the percentage of other compositions is calculated by mass.Culture medium is put into the fermentation tank of 100L in proportion, with dilute sulfuric acid tune
Section pH value is 6.1-6.3；It is passed through steam and maintains the temperature at 121 DEG C of sterilization 20min, is then cooled to 37 DEG C of access plant breasts
Bacillus species, inoculum concentration 2%, keeps constant-temperatureanaerobic anaerobic culture, agitator speed 50rpm/min.PH value is detected after culture 8h
If reaching 3.6-3.8, by bacterium solution dilute 26 times after with spectrophotometer detection bacterium solution OD values reach 0.12 ± 0.01 when, table
Show zymotic fluid fermentation stability, obtain the zymotic fluid of lactobacillus plantarum.
6) pack：Moist particle after granulation is packed using one-way ventilating valve packaging bag, ensure that strain follow-up
Fermentation process in, the gas of generation can timely be discharged by check valve, and the gas with miscellaneous bacteria of outside can not
Into in bag；The moisture of feed after granulation is microorganism and the optimum growth humidity of enzyme preparation in 28%-45%；
Packaging, which is placed in 28-32 DEG C of ferment at constant temperature workshop, ferments three days to obtain the final product.：
Embodiment 6：
Preparation method is essentially identical, and difference is the feed formula using embodiment 2, and 4-A) inoculation fermentation bacterium solution
Process for preparation in：By volume by 70% EM bacterium bacterium solutions, 20% candida utili bacterium solution；10% lactobacillus plantarum
Bacterium solution is uniformly mixed to obtain mixed fermentation bacterium solution in fermentation tank.
Embodiment 7：
Preparation method is essentially identical, and difference is the feed formula using embodiment 3, and 4-A) inoculation fermentation bacterium solution
Process for preparation in：70%EM bacterium bacterium solution by volume, 15% candida utili bacterium solution；15% lactobacillus plantarum bacterium solution
Mixed fermentation bacterium solution is uniformly mixed to obtain in fermentation tank.
Embodiment 8：
Preparation method is essentially identical, and difference is the feed formula using embodiment 2, and 4-A) inoculation fermentation bacterium solution
Process for preparation in：By volume by 75% EM bacterium bacterium solutions, 10% candida utili bacterium solution；15% lactobacillus plantarum
Bacterium solution is uniformly mixed to obtain mixed fermentation bacterium solution in fermentation tank.
Testing experiment：
A) Soak Test in water：
The finished feed that embodiment 5-8 is prepared, carries out soak test in water, while sets commercially available plain particles type
Feed (Nantong bar major company (logical prestige is controlled interest)) carries out check experiment；Test method will be specifically, will
Embodiment 5-8 is prepared feed and commercially available plain particles type feed while puts into respectively in corresponding basin,
Record initial input is 500g, pulls out, drains away the water after being soaked after input water, weighs weight A0, and records initial weight, then
Throw in backwater, take out and drain away the water respectively at 2h, 4h, 6h, 8h, 10h, weigh weight and obtain A2, A4, A6, A8, A10；With A2/A0,
A4/A0, A6/A0, A8/A0, A10/A0 keep percentage come the feed calculated respectively by the corresponding time；Specific data such as table 1
It is shown：
Table 1：Embodiment 5-8 and comparative example feed throw the contrast of water rear stability
| Percentage | Embodiment 5 | Embodiment 6 | Embodiment 7 | Embodiment 8 | Commercially available comparative example |
| 2h | 1 | 1 | 1 | 1 | 1 |
| 4h | 1 | 1 | 1 | 1 | 0.97 |
| 6h | 0.98 | 0.99 | 0.98 | 0.98 | 0.90 |
| 8h | 0.92 | 0.92 | 0.92 | 0.90 | 0.80 |
| 10h | 0.87 | 0.87 | 0.88 | 0.86 | 0.70 |
After tested, the feed stability in water that prepared by embodiment 1-4 reaches more than 10h, and commercially available plain particles type
The stability of feed is substantially in a disadvantageous position, and poor stability has been embodied in 8h.
B) fresh-keeping test in water：
The finished feed that embodiment 5-8 is prepared, carries out fresh-keeping test in water, while sets commercially available plain particles type
Feed (the Penaeus Vannmei pellet shrimp liver of big (the logical prestige is controlled interest) bio tech ltd in Nantong bar is strong) carries out control examination
Test；Test method is specifically, input is burnt accordingly respectively at the same time by the feed of embodiment 5-8 and commercially available plain particles type feed
In cup, record input amount is 100g, then respectively adds pond culture water 500mL, and it is 30 DEG C to be positioned over set temperature in insulating box
Culture.Respectively at 0h, 24h, 48h, 72h, the smell for taking out news test specimen.
Table 2：Fresh-keeping test in embodiment 5-8 and comparative example feed water
From table 2 it can be seen that fermented feed can allow beneficial bacterium to occupy excellent in the water body for being adapted to aquatic animal growth
Gesture, the growth of quick Reproduction suppression harmful bacteria, even if aquatic animal feeding is incomplete, does not have remaining fermentation material also not in water body
Eutrophication can be caused to water body, make harmful bacteria amount reproduction.And conventional feed is because without active beneficial bacterium and enzyme system
Agent, becomes the good culture medium of bacterium, in a short time, harmful bacteria is quickly bred, and causes water body harmful bacteria to surpass after putting into water body
Mark, damages to aquatic animal.
C) feed index test：
The finished feed that embodiment 5-8 is prepared, carries out feed index test, while sets commercially available common powdery hair
Ferment feed (shrimp of big (the logical prestige is controlled interest) bio tech ltd in Nantong bar is how precious) and granular pattern feed (big (the logical prestige in Nantong bar
It is holding) the Penaeus Vannmei pellet shrimp liver of bio tech ltd is strong) carry out check experiment.
Testing index：Total bacteria count content, lactic acid, pH value, soluble protein and small peptide content：
Detection method and computational methods：
I) the measure of total bacteria count content：Detection method and computational methods：
I) the measure of total bacteria count content：Carried out using colony counting method.
II) lactic acid content measures：
(1) take fermentation after fresh sample 15g be placed in 150mL beakers add be dissolved in 100mL deionized waters, in magnetic stirring apparatus
Upper extraction 30min；
(2) part extraction sample centrifugation 10min (3000r/min) is taken；
(3) supernatant 15mL is taken, adds the dilution of 30mL deionized waters to add phenolphthalein indicator four (eliminate the influence of background color)
Drop, is titrated with 0.1molNaOH standard solution, and recorded terminal consumption NaOH volumes.(terminal to solution presents pink)
(4) calculate：Lactic acid (%)=N (NaOH) × V (NaOH) × 0.09008/15 × 115/15g N (NaOH):NaOH
The concentration of standard solution；V(NaOH)：Consume NaOH standard solution volumes；0.09008：The milliequivalent of lactic acid.
III) the measure of pH value：Sample 10g is weighed in the beaker of 50mL, pipettes 15mL deionized waters, stirs 30min,
Tested with the pH value of pHS-3C types pH meter measure solution or with accurate pH test paper.
IV) Soluble Protein Contents measure：The method that dissolving index of protein is measured according to AOCSBa11-65：Weigh 20g samples
Product are in the homogenate cup of 300mL, the deionized water that measures 50mL 37+1 DEG C in homogenate cup, homogenate cup is placed on to 37 DEG C of water
In bath, soaking and stirring 5min, 10min is homogenized in interior cut type tissue refiner, and slurries are taken out from homogenate cup and move into 600mL burnings
In cup, after slurries layering, remove in 40mL supernatants injection 50mL centrifuge tubes, and centrifuged under the rotating speed of 2700r/min
10min, pipettes 15mL supernatants in kjeldahl flask, measures protein content and sample total protein content in supernatant, calculates
Dissolve the quantity of soluble protein.
V) the measure of small peptide content：Using trichloroacetic acid (TCA) method, sunk using the protein of macromolecular in TCA solution
Form sediment, remove sour insoluble protein, then the molten protein content of measure acid.External great mass of data shows the research in proteolytic
Middle measure degree of hydrolysis, adds TCA solution usually in enzymolysis liquid, is the macro-molecular protein precipitation for hydrolysis, and and small molecule
Sour molten protein ingredient, i.e. peptides and FAA leave, and the molten albumen of measure acid accounts for the content of total protein, tries to achieve degree of hydrolysis, i.e., sour molten egg
The white percentage for accounting for total protein.
Test result：It please participate in shown in table 3.
Table 3：Embodiment 5-8 and the contrast of comparative example feed index test
From table 3 it can be seen that the moist microorganism particle fermented feed of the invention prepared and conventional granulates material and fermentation
Powder is compared, and containing substantial amounts of beneficial bacterium, beneficial bacterium into digestive system occupies advantage after being searched for food by aquatic animal, suppresses harmful
The growth of bacterium makes the enteron aisle of aquatic animal more healthy, and moist microorganism particle fermented feed passes through the fermentation process of early period, feed
In partially protein be broken down into small peptide, beneficial bacterium produces substantial amounts of lactic acid in reproductive process, reduces enteron aisle acid-base value, carries
The high digestive utilization ratio of feed.Moist microorganism particle fermented feed, can be quickly by aquatile compared with the powder that ferments
Feeding, into enteron aisle, improves the enteron aisle of aquatic animal, improves the digestibility of feed, reduce the discharge of ammonia nitrogen.In excreta
Containing substantial amounts of beneficial bacterium, can be very good to improve water body.And the powder that ferments, a kind of improver of water quality can only be used as in water body
Use.And moist microorganism particle fermented feed, it can eat or improve water quality.
D index test) is cultivated：
This test purpose is that studying moist microbial fermentation pellet using the present invention cultivated aquatic animal
The influence of growth indexes and the influence to cultivation water in journey.
Subjects：The finished feed that embodiment 5-8 is prepared, while comparative example 1 is set：Commercially available common powdery fermentation
Feed (shrimp of big (the logical prestige is controlled interest) bio tech ltd in Nantong bar is how precious)
Comparative example 2：Granular pattern feed (the Penaeus Vannmei particle of big (the logical prestige is controlled interest) bio tech ltd in Nantong bar
Feed shrimp liver is strong) carry out check experiment；
Test site：Nantong Ru Dong ring agriculture Penaeus Vannmei huts farm carries out culture experiment, each breeding water body 450
Cube water body, amounts to 10 water bodys, is divided into 5 groups, every group of 2 water bodys, puts situation in a suitable place to breed and feeds ratio and is shown in Table 4：
4 shrimp seedling of table puts situation in a suitable place to breed and fermented feed feeds ratio
Result of the test：Prawn growing state is as shown in table 5：
5 litopenaeus vannamei body of table is long, body weight increase situation
In whole implementation process, it is observed that embodiment 5-8 group shrimp body colours are transparent, liver pancreas is clear-cut, and color is red
Brown, enteron aisle is full and excrement length is basically identical.And commercially available 1 shrimp body color of comparative example is deeper, part body colour is micro- red, black spots
Point is more, and liver pancreas is clear-cut, and volume is bigger than normal, and color is partially red, and enteron aisle is compared with thin and excrement length is inconsistent and color is deep.It is commercially available
2 shrimp body color of comparative example is relatively transparent, and part body colour is micro- red, and black splotch is more on crust, and enteron aisle is relatively thin and excrement length differs
Cause and color is deep.
In addition as can be seen from Table 5, prawn of the embodiment of the present invention speed of growth will be apparently higher than commercially available comparative example 1 and commercially available
Comparative example 2.The particularly second half section (30-56d) of culture experiment, group of embodiment of the present invention shrimp seedling is averaged, and body is long, body weight increase reaches
To 0.16cm/d, 0.26g/d, to when experiment at the end of, embodiment group shrimp average weight reach 9.1-9.6g/ tails (52-54 tails/
Jin, and commercially available comparative example 1,2 shrimp weight are 8.0-8.3g/ tails (60-62 tails/jin).
Feed efficiency refer to 6 data of table：
6 feed coefficient of table
| Group | Prawn harvest yield (Kg) | Total feeding volume (Kg) | Feed efficiency |
| Embodiment 5 | 765 | 653 | 117 |
| Embodiment 6 | 740 | 635 | 116 |
| Embodiment 7 | 725 | 635 | 114 |
| Embodiment 8 | 745 | 648 | 115 |
| Commercially available comparative example 1 | 637 | 640 | 99 |
| Commercially available comparative example 2 | 650 | 625 | 104 |
As can be seen from Table 6, embodiment group Feed efficiency reaches 114-116%；And commercially available comparative example 1,2 Feed efficiencies are
99-104%.
Pond water quality test is additionally carried out, data are as shown in table 7：
7 pond water quality situation of change of table
As can be seen from Table 7, embodiment group pond water quality in whole breeding process is relatively stablized, especially to prawn
Cultivation endangers larger cyanobacteria and does not break out, and main cause is to the addition of phytase in feed, reduces Phos in feed
Content, then reduces the content of phosphorus in excrement, and a large amount of beneficial bacteriums contained in feed, largely has into water body consumption
Machine matter, makes breeding water body reach bacterium algae balance.Commercially available comparative example 1 and commercially available comparative example 2, have all broken out cyanobacteria in the cultivation later stage,
Commercially available comparative example 1 is broken out more severe, and during regulating water quality, commercially available comparative example 1 and commercially available comparative example 2 have used more
More water quality improvement products maintains the stabilization of water environment.
It should be pointed out that above embodiment is only illustrative of the invention and is not intended to limit the scope of the invention,
After having read the present invention, modification of the those skilled in the art to the various equivalent forms of the present invention falls within power appended by the application
Profit requires limited range.
Claims (10)
- A kind of 1. moist microorganism particle fermented feed for aquatic livestock, it is characterised in that fermented feed is graininess, its Particle diameter is 0.3mm-6.0mm, feed ingredient two and 0.02- calculated by mass including 4-7 parts of feed ingredient one, 3-6 parts 0.05 part of feed ingredient three；Each material quality percentage is as follows in wherein described feed ingredient one：Peanut meal 20%-40%, dregs of beans 30%-50%, dish Dregs of rice 5%-10%, chicken meal 0-7%, blood meal 2%-6%, hydrolyzed feather meal 1%-3%, rice bran 6%-10%；Each material quality percentage is as follows in the feed ingredient two：Krill meal 2%-6%, yeast extract 2%-6%, cuttlefish Cream 6%-10%, Strong flour 50%-80%, calcium dihydrogen phosphate 1%-2%, binding agent 0.1%-1%, salt 0.5%-1%, Zeolite powder 5%-14%；Each material quality percentage is as follows in the feed ingredient three：Glucose oxidase 10%-50%, DP100 protease 30%-60%, cellulase 20%-50%, phytase 20%-40%.
- 2. the moist microorganism particle fermented feed according to claim 1 for aquatic livestock, it is characterised in that：It is described The Strong flour ratio calculated by mass for accounting for the fermented feed gross mass is more than 30%.
- 3. the moist microorganism particle fermented feed according to claim 1 for aquatic livestock, it is characterised in that：It is described Binding agent is specially sodium alginate.
- 4. the moist microorganism particle fermented feed according to claim 1 for aquatic livestock, it is characterised in that：It is described Yeast extract using food yeast as raw material, using self-dissolving, enzymolysis, separation, concentration technique by the protein in yeast cells, Nucleic acid etc. is refined after being degraded and formed；Main component is polypeptide, amino acid, flavour nucleotide, B family vitamin and micro member Element.
- 5. the moist microorganism particle fermented feed according to claim 1 for aquatic livestock, it is characterised in that：It is described The moisture of fermented feed is 25%-45%.
- 6. a kind of preparation method of moist microorganism particle fermented feed for aquatic livestock as claimed in claim 1, its It is characterized in that including the following steps：1) weigh：The preparing raw material in clean each fermented feed component is weighed by formula regulation number；2) pre-treatment：2-A) mix：Three kinds of fermented feed components are carried out to separate mixed processing；Wherein feed ingredient one is mixed at room temperature using single shaft paddle formula mixer, and mixability is the variation lines of material Number is less than 5%；It is then sent to water droplet formula pulverizer to be crushed, powder particle diameter is spare for 50-60 mesh；Feed ingredient two carries out mixing for standby use using horizontal-type biaxial paddle formula mixer；Feed ingredient three carries out mixing for standby use using drum mixer；2-B) ultramicro grinding：The feed ingredient one after crushing is weighed in proportion and feed ingredient two puts into horizontal-type biaxial paddle formula and mixes Conjunction machine is mixed at room temperature；Put into ultra micro filler bin after mixing to be crushed with vertical shaft type micronizer, grinding particle size Reach more than 100 mesh, obtain the mixed material A being made of feed ingredient one and feed ingredient two；3) expanding treatment：The mixed material A input expanding treatment equipment that step 2) is obtained carries out expanding treatment：It is swollen using wet method Swollen method, it is 125-138 DEG C to control swelling temperature, and pressure is 34-37 atmospheric pressure, and moisture is controlled in 20%-25%；4) pelletization treatment：Mixed material A after step 3) expanding treatment is pelletized in bulking equipment；Temperature 100-140 DEG C, compression ratio 20-28, screw speed 500-800rpm, moisture 22%-26%；Pellet after granulation is as cold as 25-35 It is spare after DEG C；5) fermentation process：5-A) the preparation of inoculation fermentation bacterium solution：Three kinds of EM bacterium, candida utili, lactobacillus plantarum zymocyte liquids are prepared respectively； According to the ratio of bacterium solution volume；Wherein by volume by the EM bacterium solutions of 30%-80%, the candida utili bacterium of 10%-30% Liquid；The lactobacillus plantarum bacterium solution of 10%-60% is uniformly mixed to obtain mixed fermentation bacterium solution in fermentation tank；Mixing in step 2) is standby Feed ingredient three is added in mixed fermentation bacterium solution according to required ratio, is uniformly mixing to obtain inoculation fermentation bacterium solution；5-B) combined inoculation：The spare mixed material A of step 4) granulation postcooling is put into plough mixer, step 5-A) system The inoculation fermentation bacterium solution obtained is according to the mixing in ratio spray inoculation to the plough mixer of the 5%-15% of mixed material A mass Finished feed is obtained in material A；6) pack：Moist particle is packed using one-way ventilating valve packaging bag, and the moisture of the feed after granulation is in 28%- Between 45%, packaging, which is placed in 28-32 DEG C of ferment at constant temperature workshop, ferments three days to obtain the final product.
- 7. the preparation method of the moist microorganism particle fermented feed according to claim 1 for aquatic livestock, it is special Sign is：Step 5-A) in EM bacterium bacterium solution prepare medium component and proportioning calculated by mass be：Peptone 1%, sugar Honey 10%, potassium dihydrogen phosphate 0.05%, water surplus；PH is 7.0；EM strains inoculum concentration is the 5% of culture medium gross mass.
- 8. the preparation method of the moist microorganism particle fermented feed according to claim 1 for aquatic livestock, it is special Sign is：Step 5-A) in candida utili bacterium solution prepare medium component and proportioning calculated by mass be：Corn Slurry 0.5%, molasses 0.5%, glucose 2.0%, KH2P040.1%th, (NH4)2SO41%th, water surplus；PH is 6.0-6.5；Produce protein Candida strain inoculum concentration is the 2% of culture medium gross mass.
- 9. the preparation method of the moist microorganism particle fermented feed according to claim 1 for aquatic livestock, it is special Sign is：Step 5-A) in lactobacillus plantarum bacterium solution prepare medium component and proportioning be：Beef extract 0.6%, molasses 0.8%th, potassium dihydrogen phosphate 0.2%, Triammonium citrate 0.2%, Tween 80 0.1%, water surplus, pH 6.1-6.3；Plant breast Bacillus species inoculum concentration be culture medium gross mass 2%, wherein except Tween 80 percentage in terms of volume, the hundred of other compositions Divide than calculated by mass.
- 10. the preparation method of the moist microorganism particle fermented feed according to claim 1 for aquatic livestock, it is special Sign is：Facility for granulating described in step 4) uses stainless steel ring die pelletizing equipment, and pressure roller is serration；Power of motor main motor is Six grades.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810122641.2A CN108013213A (en) | 2018-02-07 | 2018-02-07 | A kind of moist microorganism particle fermented feed for aquatic livestock and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810122641.2A CN108013213A (en) | 2018-02-07 | 2018-02-07 | A kind of moist microorganism particle fermented feed for aquatic livestock and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108013213A true CN108013213A (en) | 2018-05-11 |
Family
ID=62075329
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810122641.2A Pending CN108013213A (en) | 2018-02-07 | 2018-02-07 | A kind of moist microorganism particle fermented feed for aquatic livestock and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108013213A (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108142642A (en) * | 2018-03-15 | 2018-06-12 | 山东碧蓝生物科技有限公司 | A kind of nutrition immune type prawn soft pellet diet and preparation method thereof |
| CN109221810A (en) * | 2018-09-27 | 2019-01-18 | 淮安正昌饲料有限公司 | A kind of aquatic product matched feed of biofermentation |
| CN110338275A (en) * | 2019-08-05 | 2019-10-18 | 通威股份有限公司 | A kind of extrusion type biological fermentation feed and its preparation and application method |
| CN110710617A (en) * | 2019-10-11 | 2020-01-21 | 青岛尚德生物技术有限公司 | Fermented soft pellet feed for penaeus vannamei boone and preparation method and application thereof |
| CN110904003A (en) * | 2019-11-29 | 2020-03-24 | 博益德（北京）生物科技有限公司 | Compound fermentation inoculant for aquatic products and preparation method and application thereof |
| CN111406830A (en) * | 2020-04-07 | 2020-07-14 | 湖北希普生物科技有限公司 | Preparation method of compound microbial fermentation puffed feed |
| CN111567674A (en) * | 2020-05-13 | 2020-08-25 | 李春雨 | Feed processing method |
| CN111772056A (en) * | 2020-07-07 | 2020-10-16 | 深圳市澳华集团股份有限公司 | Functional fermented puffed feed for aquatic animals and production method and device thereof |
| CN111820316A (en) * | 2020-06-30 | 2020-10-27 | 新疆天康饲料有限公司 | Fermented puffed pellet feed for early stage of overwintering grass carp culture and preparation method thereof |
| CN112044584A (en) * | 2020-08-27 | 2020-12-08 | 程亮 | Probiotics draws raw materials processing apparatus |
| CN112205545A (en) * | 2020-10-23 | 2021-01-12 | 安徽天邦饲料科技有限公司 | Preparation method of liver-protecting intestine-protecting fermented penaeus vannamei granule |
| CN114651914A (en) * | 2022-03-22 | 2022-06-24 | 河南省水产科学研究院 | Special feed for comprehensive planting and breeding of rice and fish in saline-alkali water area and processing technology thereof |
| CN115176933A (en) * | 2022-06-17 | 2022-10-14 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Compound feed for marine fish fermented after granulation and preparation method thereof |
| CN117882803A (en) * | 2024-03-18 | 2024-04-16 | 四川省畜牧科学研究院 | Preparation method of organic trace element compound premix for piglets |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102422991A (en) * | 2011-10-20 | 2012-04-25 | 郑诚 | Biologically fermented puffed particle forage for pigling and preparation method thereof |
| CN104431327A (en) * | 2014-12-11 | 2015-03-25 | 天津现代天骄水产饲料股份有限公司 | Preparation method of aquatic puffed feed |
| CN104770596A (en) * | 2015-04-07 | 2015-07-15 | 中国水产科学研究院珠江水产研究所 | Aquatic animal intestinal regulator and application thereof |
| CN105211578A (en) * | 2015-11-03 | 2016-01-06 | 江苏省淡水水产研究所 | A kind of Procambius clarkii juvenile prawn environment-friendly type mixed feed and feeding method thereof |
| CN105746883A (en) * | 2016-03-03 | 2016-07-13 | 南京致润生物科技有限公司 | Active fermentation cold-granulated feed as well as production method and application thereof |
| CN106689796A (en) * | 2015-11-13 | 2017-05-24 | 江苏银宝生物科技有限公司 | Black carp biological feed formula and processing method |
-
2018
- 2018-02-07 CN CN201810122641.2A patent/CN108013213A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102422991A (en) * | 2011-10-20 | 2012-04-25 | 郑诚 | Biologically fermented puffed particle forage for pigling and preparation method thereof |
| CN104431327A (en) * | 2014-12-11 | 2015-03-25 | 天津现代天骄水产饲料股份有限公司 | Preparation method of aquatic puffed feed |
| CN104770596A (en) * | 2015-04-07 | 2015-07-15 | 中国水产科学研究院珠江水产研究所 | Aquatic animal intestinal regulator and application thereof |
| CN105211578A (en) * | 2015-11-03 | 2016-01-06 | 江苏省淡水水产研究所 | A kind of Procambius clarkii juvenile prawn environment-friendly type mixed feed and feeding method thereof |
| CN106689796A (en) * | 2015-11-13 | 2017-05-24 | 江苏银宝生物科技有限公司 | Black carp biological feed formula and processing method |
| CN105746883A (en) * | 2016-03-03 | 2016-07-13 | 南京致润生物科技有限公司 | Active fermentation cold-granulated feed as well as production method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 田建中主编: "《现代渔业养殖实用技术》", 31 December 2016, 河北科学技术出版社 * |
| 邹作宇等: "《鲟鱼高产养殖技术》", 31 July 2011, 东北林业大学出版社 * |
| 阚建全等: "《食品化学》", 30 September 2002, 中国农业大学出版社 * |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108142642A (en) * | 2018-03-15 | 2018-06-12 | 山东碧蓝生物科技有限公司 | A kind of nutrition immune type prawn soft pellet diet and preparation method thereof |
| CN109221810A (en) * | 2018-09-27 | 2019-01-18 | 淮安正昌饲料有限公司 | A kind of aquatic product matched feed of biofermentation |
| CN110338275A (en) * | 2019-08-05 | 2019-10-18 | 通威股份有限公司 | A kind of extrusion type biological fermentation feed and its preparation and application method |
| CN110710617A (en) * | 2019-10-11 | 2020-01-21 | 青岛尚德生物技术有限公司 | Fermented soft pellet feed for penaeus vannamei boone and preparation method and application thereof |
| CN110904003A (en) * | 2019-11-29 | 2020-03-24 | 博益德（北京）生物科技有限公司 | Compound fermentation inoculant for aquatic products and preparation method and application thereof |
| CN111406830A (en) * | 2020-04-07 | 2020-07-14 | 湖北希普生物科技有限公司 | Preparation method of compound microbial fermentation puffed feed |
| CN111567674A (en) * | 2020-05-13 | 2020-08-25 | 李春雨 | Feed processing method |
| CN111820316A (en) * | 2020-06-30 | 2020-10-27 | 新疆天康饲料有限公司 | Fermented puffed pellet feed for early stage of overwintering grass carp culture and preparation method thereof |
| CN111772056A (en) * | 2020-07-07 | 2020-10-16 | 深圳市澳华集团股份有限公司 | Functional fermented puffed feed for aquatic animals and production method and device thereof |
| CN112044584A (en) * | 2020-08-27 | 2020-12-08 | 程亮 | Probiotics draws raw materials processing apparatus |
| CN112044584B (en) * | 2020-08-27 | 2022-05-17 | 安徽中微微元生物科技有限公司 | Probiotics draws raw materials processing apparatus |
| CN112205545A (en) * | 2020-10-23 | 2021-01-12 | 安徽天邦饲料科技有限公司 | Preparation method of liver-protecting intestine-protecting fermented penaeus vannamei granule |
| CN114651914A (en) * | 2022-03-22 | 2022-06-24 | 河南省水产科学研究院 | Special feed for comprehensive planting and breeding of rice and fish in saline-alkali water area and processing technology thereof |
| CN114651914B (en) * | 2022-03-22 | 2024-04-19 | 河南大德生物技术有限公司 | Special feed for comprehensive planting and raising of rice and fish in saline-alkali water area and processing technology thereof |
| CN114651914B8 (en) * | 2022-03-22 | 2024-05-28 | 河南省水产科学研究院 | Special feed for comprehensive planting and raising of rice and fish in saline-alkali water area and processing technology thereof |
| CN115176933A (en) * | 2022-06-17 | 2022-10-14 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Compound feed for marine fish fermented after granulation and preparation method thereof |
| CN117882803A (en) * | 2024-03-18 | 2024-04-16 | 四川省畜牧科学研究院 | Preparation method of organic trace element compound premix for piglets |
| CN117882803B (en) * | 2024-03-18 | 2024-06-21 | 四川省畜牧科学研究院 | Preparation method of organic trace element compound premix for piglets |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108013213A (en) | A kind of moist microorganism particle fermented feed for aquatic livestock and preparation method thereof | |
| CN106173363B (en) | The method for producing cattle and sheep complete feed as raw material staged fermentation using edible fungus bran | |
| CN108244407A (en) | It is a kind of for moist microorganism particle fermented feed of aquatic livestock and preparation method thereof | |
| CN103349157B (en) | Compound feed for female parent fishes of sturgeons | |
| CN101455270B (en) | Plant fermentation feedstuff | |
| CN110338275A (en) | A kind of extrusion type biological fermentation feed and its preparation and application method | |
| CN108041298A (en) | Piglet fermented type mixed feed | |
| CN103300246A (en) | Feed for increasing milk of lactating sows and preparation method for feed | |
| CN102987140A (en) | Method for preparing novel ruminant feed by using beneficial bacteria and plant fiber | |
| CN110731417B (en) | Microbial fermentation feed for crayfish and preparation method thereof | |
| CN103385350B (en) | Soft particle milk replacer containing high fructose corn syrup and preparation thereof | |
| CN104886390A (en) | Biologically fermented pig feed and a preparation method | |
| CN105010879A (en) | Cassava residue fermentation pork pig feed and preparation method thereof | |
| CN108835403A (en) | A kind of sheep full price hybrid particles feed and preparation method thereof | |
| CN102178091A (en) | Baking type suckling pig feed before weaning and preparation method thereof | |
| CN104186939A (en) | Ruminant animal feed and preparation method thereof | |
| CN106071143B (en) | A kind of granular pattern probiotics and preparation method thereof that ruminant production performance can be improved | |
| CN103349156A (en) | Oligopeptide-rich powder-type compound feed for anguilla marmorata | |
| CN103262959B (en) | Puffing settleable mixed feed for adult fish of fugu obscurus | |
| CN106561991A (en) | Method of preparing granular feed for beef cattle by means of straw or leaf tails of sugarcane | |
| CN1969658B (en) | Biological organic bait containing ferment bacteria for bighead carp and method for preparing same | |
| CN102396652A (en) | Complex enzyme preparation for aquatic animal and preparation process thereof | |
| CN110169506A (en) | A kind of turmeric dreg fermentation pannage and preparation method thereof | |
| CN105341508A (en) | Puffing feed for carassius auratus gibelio and preparation method of puffing feed | |
| CN107467414A (en) | A kind of compound feed additive of probiotics fermention natural grain |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180511 |
|
| RJ01 | Rejection of invention patent application after publication |