CN110050877A - A kind of Heisui River gadfly slurry semisolid concentrated feed and preparation method - Google Patents
A kind of Heisui River gadfly slurry semisolid concentrated feed and preparation method Download PDFInfo
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- CN110050877A CN110050877A CN201910334083.0A CN201910334083A CN110050877A CN 110050877 A CN110050877 A CN 110050877A CN 201910334083 A CN201910334083 A CN 201910334083A CN 110050877 A CN110050877 A CN 110050877A
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- generation
- slurry
- heisui river
- semisolid
- river gadfly
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- 239000002002 slurry Substances 0.000 title claims abstract description 82
- 238000002360 preparation method Methods 0.000 title claims abstract description 47
- 241000894006 Bacteria Species 0.000 claims abstract description 112
- 241000193171 Clostridium butyricum Species 0.000 claims abstract description 73
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 61
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 59
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 58
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 58
- 241001481656 Stratiomyidae Species 0.000 claims abstract description 36
- 240000008042 Zea mays Species 0.000 claims abstract description 31
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 31
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 30
- 235000005822 corn Nutrition 0.000 claims abstract description 30
- 235000013312 flour Nutrition 0.000 claims abstract description 30
- 235000013379 molasses Nutrition 0.000 claims abstract description 17
- 239000007788 liquid Substances 0.000 claims description 120
- 239000001963 growth medium Substances 0.000 claims description 66
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 28
- 238000000855 fermentation Methods 0.000 claims description 25
- 230000004151 fermentation Effects 0.000 claims description 25
- 239000013587 production medium Substances 0.000 claims description 23
- 239000002994 raw material Substances 0.000 claims description 22
- 239000002054 inoculum Substances 0.000 claims description 21
- 229920002472 Starch Polymers 0.000 claims description 19
- 235000019698 starch Nutrition 0.000 claims description 19
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 18
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 18
- 235000013372 meat Nutrition 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000004615 ingredient Substances 0.000 claims description 15
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 241000196324 Embryophyta Species 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 10
- 238000004140 cleaning Methods 0.000 claims description 9
- 241000186660 Lactobacillus Species 0.000 claims description 8
- 235000012907 honey Nutrition 0.000 claims description 8
- 229940039696 lactobacillus Drugs 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- VLSOAXRVHARBEQ-UHFFFAOYSA-N [4-fluoro-2-(hydroxymethyl)phenyl]methanol Chemical compound OCC1=CC=C(F)C=C1CO VLSOAXRVHARBEQ-UHFFFAOYSA-N 0.000 claims description 7
- 229940099596 manganese sulfate Drugs 0.000 claims description 7
- 239000011702 manganese sulphate Substances 0.000 claims description 7
- 235000007079 manganese sulphate Nutrition 0.000 claims description 7
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 7
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 7
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 7
- 230000000284 resting effect Effects 0.000 claims description 7
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 claims description 5
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 claims description 5
- 235000012538 ammonium bicarbonate Nutrition 0.000 claims description 5
- 239000001099 ammonium carbonate Substances 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 4
- 239000006071 cream Substances 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims 1
- 239000005864 Sulphur Substances 0.000 claims 1
- 239000011777 magnesium Substances 0.000 claims 1
- 229910052749 magnesium Inorganic materials 0.000 claims 1
- 230000000050 nutritive effect Effects 0.000 abstract description 13
- 241001465754 Metazoa Species 0.000 abstract description 6
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 5
- 230000007774 longterm Effects 0.000 abstract description 5
- 238000004321 preservation Methods 0.000 abstract description 5
- 238000010438 heat treatment Methods 0.000 abstract description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 12
- 238000000034 method Methods 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 10
- 108090000765 processed proteins & peptides Proteins 0.000 description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 8
- 239000000463 material Substances 0.000 description 7
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 6
- 235000019341 magnesium sulphate Nutrition 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 239000008107 starch Substances 0.000 description 6
- 235000007926 Craterellus fallax Nutrition 0.000 description 5
- 240000007175 Datura inoxia Species 0.000 description 5
- 235000019733 Fish meal Nutrition 0.000 description 5
- 240000007594 Oryza sativa Species 0.000 description 5
- 235000007164 Oryza sativa Nutrition 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 5
- 239000004467 fishmeal Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 235000009566 rice Nutrition 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 241000251468 Actinopterygii Species 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 244000241257 Cucumis melo Species 0.000 description 3
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 3
- 229940024606 amino acid Drugs 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 238000009360 aquaculture Methods 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 3
- 239000010977 jade Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 238000012958 reprocessing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000709785 Hermetia illucens Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 229920000294 Resistant starch Polymers 0.000 description 1
- 241000190950 Rhodopseudomonas palustris Species 0.000 description 1
- 241000255632 Tabanus atratus Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229940036811 bone meal Drugs 0.000 description 1
- 239000002374 bone meal Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 235000021254 resistant starch Nutrition 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000010563 solid-state fermentation Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of Heisui River gadfly slurry semisolid concentrated feed and preparation methods, belong to fermented feed field.Aiming at the problem that the short and higher cost as the feed shelf-life of black soldier flies in the prior art, the present invention provides a kind of Heisui River gadfly slurry semisolid concentrated feed, it includes each component of following mass percent: Heisui River gadfly slurry 86~88%, lactobacillus plantarum 2 generation bacterium solution 3~4%, clostridium butyricum 2 generation bacterium solution 3~4%, saccharomycete 2 generation bacterium solution 1~2%, corn flour 1~2% and molasses 2~3%.The black soldier flies semisolid concentrated feed nutritive value is high, can promote the absorptivity of animal intestinal tract, cost is relatively low, and the feed can long-term preservation；And a kind of preparation method of Heisui River gadfly slurry semisolid concentrated feed provided by the invention, without additionally heating, it is easy to operate, so that the purposes of black soldier flies is no longer limited to worm feeding living or dries the usage mode as higher costs such as feeds, improve the utilization rate of black soldier flies.
Description
Technical field
The invention belongs to fermented feed field, more specifically to a kind of Heisui River gadfly starch semisolid concentrated feed and
Preparation method.
Background technique
Fermented feed is the hot topic of present feed industry, after undergoing microbial fermentation, improves free amino acid and small
The content of peptide, it may be difficult to which the substance directly absorbed is converted into the nutritional ingredient that animal can be absorbed, and beneficial microbe can
It plays a protective role to intestinal health, fermented feed is increasingly paid attention to by current cultivation industry；Traditional fish feed, if using
Then palatability reduces too many low value feed；If cost increases, and easily causes to bear to organs such as livers using high protein feed
Load, influences healthy fish.
It is also correspondingly improved regarding to the issue above, it is open such as Chinese Patent Application No. CN201611102672.9
Day is on June 12nd, 2018, which is related to a kind of fish fermented feed, it is mainly made of following raw material: palm kernel meal, rice
Chaff, maize peel, wheat bran, distillers ' grains and leavening.When fish of the invention is prepared with fermented feed, each feedstuff is first distinguished into powder
It is broken, it is sieved, mixing；Leavening is uniformly mixed with feedstuff mixture again；It is added the 40-60%'s of weight of material later
Water is uniformly mixed by water with feedstuff mixture；It finally fills this blend into closed container or is carried out in respiratory membrane bag
Fermentation.The present invention generates organic acid, various enzymes, amino acid, vitamin, polysaccharide isoreactivity substance by fermentation, and it is suitable to improve feed
Mouth property is green, a kind of environmentally friendly, healthy aquaculture important way.The patent is disadvantageous in that: made fermented feed
Nutritive value is lower, and the field of application is more single；And fermented feed production is relatively complicated.
For another example Chinese Patent Application No. CN201510076705.6, publication date are on May 13rd, 2015, which disclose
A kind of fermented feed.It is fermented to obtain by following raw materials through microorganism formulation is added: potato 20-25%, corn 20-18%, beans
Dregs of rice 5-6%, bone meal 2%, bean dregs 38-34%, vegetables 4-6%, melon seedling or peanut vine 10-8%, composite trace element 1%；Its
Middle microorganism formulation is the mixing of bacillus amyloliquefaciens, lactobacillus plantarum, bacillus subtilis, Rhodopseudomonas palustris
Object.The present invention passes through the raw materials such as mentioned microorganism preparation fermentation potato, bean dregs and ground melon seedling, the resistant starch in potato of degrading,
Crude fibre and protein in bean dregs, the crude fibre in ground melon seedling, improve the utilization rate of feed cheap raw material, significantly reduce
Feed cost increases culture efficiency.The patent is disadvantageous in that: fermented fodder nutrient obtained is lower.
Black soldier flies (Hermetiaillucens.L) are a kind of septic insects, because that can convert organic waste rapidly
For the high-quality protein that can cook food and feed, and used by more and more all kinds of environmental protection and feed industries exploitation, current Heisui River
Feed addition is done in the worm feeding predominantly living of the utilization mode of horsefly commercial insect and drying, wherein worm living is maximum the disadvantage is that the shelf-life
It is short, save loss greatly, the electricity charge consumption directly dried is huge, accounts for 1/4 of profit or more；But current black soldier flies commercial insect lacks
The market of mass efficient is supported, the thinking of Fodder making product is lacked, and the present invention then overcomes the black soldier flies shelf-life short and makees feed
Difficulty at high cost provides a kind of Heisui River gadfly slurry solid state fermentation concentrated feed and preparation method.
Summary of the invention
1. to solve the problems, such as
Aiming at the problem that the short and higher cost as the feed shelf-life of black soldier flies in the prior art, the present invention provides a kind of black
Stratiomyiid worm starches semisolid concentrated feed and preparation method.The feed can promote animal intestinal tract absorptivity and nutrition with higher
Value, being capable of long-term preservation；A kind of Heisui River gadfly provided by the invention starches semisolid concentrated feed preparation method, easy to operate,
Additional aids are not needed, it is at low cost without heating.
2. technical solution
To solve the above-mentioned problems, the technical solution adopted in the present invention is as follows:
A kind of Heisui River gadfly slurry semisolid concentrated feed, each component including following mass percent: Heisui River gadfly slurry 86
~88%, lactobacillus plantarum 2 generation bacterium solution 3~4%, clostridium butyricum 2 generation bacterium solution 3~4%, saccharomycete 2 generation bacterium solution 1~2%, corn
Powder 1~2% and molasses 2~3%.
Further, the culture medium of 2 generation of the lactobacillus plantarum bacterium solution and saccharomycete 2 generation bacterium solution is easy expansion culture
Base, easy expansion culture medium include the ingredient of following mass percent: molasses 2~3%, corn flour 1~2%, gruel 5~8%
With calcium carbonate 0.05~0.1%, remaining is water.
Further, the culture medium of 2 generation of the clostridium butyricum bacterium solution is production medium, and production medium includes as follows
The ingredient of mass percent: dregs of beans 1.5~2%, fermented bean dregs 0.3~0.5%, glucose 1~2%, corn flour 1~2%, chlorine
Change sodium 0.03~0.05%, calcium carbonate 0.03~0.05%, potassium dihydrogen phosphate 0.02~0.03%, dipotassium hydrogen phosphate 0.02~
0.03%, ammonium hydrogen carbonate 0.02~0.03%, magnesium sulfate 0.02~0.03%, manganese sulfate 0.01~0.02% and L-cysteine
Hydrochloride 0.02~0.03%, remaining is water.
A kind of preparation method of Heisui River gadfly slurry semisolid concentrated feed, comprising the following steps:
(1) lactobacillus plantarum 2 generation bacterium solution, clostridium butyricum 2 generation bacterium solution and saccharomycete 2 generation bacterium solution are prepared；
(2) black soldier flies larva is placed 2~3 days under field conditions (factors), by black soldier flies children after being cleaned by microwave cleaning machine
Worm breaks into worm and starches to obtain Heisui River gadfly slurry；
(3) in proportion by Heisui River gadfly slurry, corn flour, lactobacillus plantarum 2 generation bacterium solution, clostridium butyricum 2 generation bacterium solution and honey
It places after mixing in a reservoir；
(4) saccharomycete 2 generation bacterium solution is covered on above-mentioned raw materials surface, closed container ferments, i.e. black after fermentation
Stratiomyiid worm starches the preparation of semisolid concentrated feed and completes.
Further, black soldier flies larva is broken into Heisui River gadfly slurry with meat grinder in the step (2).
Further, meat grinder discharging bore is less than 2mm and the Heisui River gadfly of preparation starches the resting period no more than
12h。
Further, include the following steps: in the step (1)
The preparation of (1.1) 1 generation strain liquids: lactobacillus plantarum, clostridium butyricum and the corresponding 1 generation strain of saccharomycete are trained
Feeding base sterilizes, and corresponding strain is aseptically inoculated with after cooling, and 37 DEG C of shaking table cultures for 24 hours, obtain lactobacillus plantarum 1
For strain liquid, clostridium butyricum 1 generation strain liquid and saccharomycete 1 generation strain liquid；
The preparation of (1.2) 2 generation strain liquids: inoculated plant lactobacillus 1 generation strain liquid and yeast on easy expansion culture medium
Bacterium 1 generation strain liquid, is inoculated with 1 generation of clostridium butyricum strain liquid in production medium, and 37 DEG C of shaking table cultures obtain plant cream bar afterwards for 24 hours
Bacterium 2 generation strain liquid, clostridium butyricum 2 generation strain liquid and saccharomycete 2 generation strain liquid.
Further, lactobacillus plantarum 1 generation bacterium culture medium is MRS culture medium, saccharomycete 1 in the step (1.1)
It is PDA culture medium for bacterium culture medium, clostridium butyricum 1 generation bacterium culture medium is RCM culture medium.
Further, lactobacillus plantarum 1 generation strain liquid inoculum concentration is 5~7% in the step (1.2), 1 generation of saccharomycete
Strain liquid inoculum concentration is 1~3%, and clostridium butyricum 1 generation strain liquid inoculum concentration is 0.2%~0.3%.
Further, temperature is controlled at 35~40 DEG C during fermentation in the step (4).
3. beneficial effect
Compared with the prior art, the invention has the benefit that
(1) slurry of Heisui River gadfly made of present invention semisolid concentrated feed, which can directly pelletize, feeding or cooperates other feeds former
Commercial feed is made in material reprocessing, and the beneficial microbe in Heisui River gadfly slurry semisolid concentrated feed can promote the suction of animal intestinal tract
It produces effects rate, worm ferment albumen can be used as the good raw material of substitution fish meal, dependence of the aquaculture to fish meal be reduced, in conjunction with the jade to low price
The raw materials such as rice flour, molasses utilize, and improve feed value, greatly promote the benefit in whole process；
(2) every trophonemata in the slurry of Heisui River gadfly made of present invention semisolid concentrated feed long-term preservation in the feed
Mark does not change, butt protein content 38%~40%, wherein free amino acid/small peptide content accounting in protein content
33.5% or more, living bacteria count > 5.6*108Cfu, so that the purposes of black soldier flies is no longer limited to worm living and feeds either baking
Dry to be used as feed, so that the purposes of black soldier flies is wider, utilization rate is higher；
(3) present invention places black soldier flies larva two days under field conditions (factors), so that black soldier flies larva sufficiently discharges into enteron aisle
Interior excrement cleans black soldier flies larva using microwave cleaning machine, is finally putting into meat grinder and breaks into worm slurry, so that final Heisui River
Gadfly slurry more cleans, without other impurity or pollutant, so that the feed finally prepared is more pure；
(4) method of present invention preparation Heisui River gadfly slurry semisolid concentrated feed is easy to operate, without additional heating, therefore saves
About electric power energy and save the cost largely；Use first prepare strain liquid again in the form of relatively large inoculation give worm slurry hair
The inoculation of ferment material, can reduce living contaminants, improve finished product probiotics purity, so that the feed nutritive value finally prepared is high.
Detailed description of the invention
Fig. 1 is preparation process figure of the invention.
Specific embodiment
A kind of Heisui River gadfly slurry semisolid concentrated feed, each component including following mass percent: Heisui River gadfly slurry 86
~88%, lactobacillus plantarum 2 generation bacterium solution 3~4%, clostridium butyricum 2 generation bacterium solution 3~4%, saccharomycete 2 generation bacterium solution 1~2%, corn
Powder 1~2% and molasses 2~3%；
Heisui River gadfly slurry semisolid concentrated feed is made by following steps:
The preparation of (1.1) 1 generation strain liquids: lactobacillus plantarum, clostridium butyricum and the corresponding 1 generation strain of saccharomycete are trained
Feeding base sterilizes, and corresponding strain is aseptically inoculated with after cooling, and 37 DEG C of shaking table cultures for 24 hours, obtain lactobacillus plantarum 1
For strain liquid, clostridium butyricum 1 generation strain liquid and saccharomycete 1 generation strain liquid；
The preparation of (1.2) 2 generation strain liquids: inoculated plant lactobacillus 1 generation strain liquid and yeast on easy expansion culture medium
Bacterium 1 generation strain liquid, is inoculated with 1 generation of clostridium butyricum strain liquid in production medium, and 37 DEG C of shaking table cultures obtain plant cream bar afterwards for 24 hours
Bacterium 2 generation strain liquid, clostridium butyricum 2 generation strain liquid and saccharomycete 2 generation strain liquid；
(2) black soldier flies larva is placed 2~3 days under field conditions (factors), by black soldier flies children after being cleaned by microwave cleaning machine
Worm breaks into worm by meat grinder and starches to obtain Heisui River gadfly slurry；
(3) by Heisui River gadfly slurry, corn flour, lactobacillus plantarum 2 generation bacterium solution, clostridium butyricum 2 generation bacterium solution and honey, these are former
Material is placed in a reservoir after mixing in proportion；
(4) saccharomycete 2 generation bacterium solution is covered on above-mentioned raw materials surface, closed container carries out fermentation 4 days or more, and fermentation is completed
It is afterwards that the slurry semisolid concentrated feed preparation of Heisui River gadfly is completed.
Continue room temperature in sealed states using the slurry semisolid concentrated feed of Heisui River gadfly made of above-mentioned method to store, deposit
It puts in 6 months, regular sample detection nutritive index and viable count, initial value and the nutritive index variation such as table 1 measured of sampling for 6 times
It is shown:
1 room temperature of table, which is stored, detects nutritive index and viable count in 6 months
| pH | Moisture | Albumen | Small peptide | Living bacteria count | |
| Initially | 4.43 | 79.52% | 39.14% | 34.12% | 5.75*10^8cfu |
| 1 month | 4.31 | 78.99% | 39.16% | 34.16% | 5.38*10^8cfu |
| 2 months | 4.23 | 78.97% | 39.16% | 34.22% | 5.16*10^8cfu |
| 3 months | 4.22 | 78.92% | 39.15% | 34.28% | 4.67*10^8cfu |
| 4 months | 4.25 | 78.94% | 39.15% | 34.35% | 4.23*10^8cfu |
| 5 months | 4.19 | 78.93% | 39.14% | 34.57% | 4.01*10^8cfu |
| 6 months | 4.22 | 78.92% | 39.11% | 34.66% | 3.35*10^8cfu |
Seen from table 1, it is kept under former sealing state using the slurry semisolid concentrated feed of Heisui River gadfly made of the above method
Room temperature continues to save, and nutritive index has no significant change in 6 months, living bacteria count still on the 10 8 power orders of magnitude, depending on
Work can be with long-term preservation；Solve the problems, such as that black soldier flies product is unable to long-term preservation, so that the more application of black soldier flies
Route, improve the utilization rate of black soldier flies；Manufactured Heisui River gadfly slurry semisolid concentrated feed, which can directly pelletize, to feed or matches
It closes the reprocessing of other feedstuffs and commercial feed is made, the beneficial microbe in Heisui River gadfly slurry semisolid concentrated feed can promote
The absorption efficiency of animal intestinal tract, worm ferment albumen can be used as the good raw material of substitution fish meal, reduce dependence of the aquaculture to fish meal, and
Whole nutritive value is higher；The preparation method solves the drying of Heisui River gadfly as feed cost is high and asks without additional heating
Topic saves power cost.
The present invention is further described below combined with specific embodiments below.
Embodiment 1
A kind of Heisui River gadfly slurry semisolid concentrated feed, each component including following mass percent: Heisui River gadfly slurry
86%, lactobacillus plantarum 2 generation bacterium solution 3%, clostridium butyricum 2 generation bacterium solution 4%, saccharomycete 2 generation bacterium solution 2%, corn flour 2% and molasses
3%；Heisui River gadfly slurry semisolid concentrated feed is made by following steps:
The preparation of (1.1) 1 generation strain liquids: lactobacillus plantarum, clostridium butyricum and the corresponding 1 generation strain of saccharomycete are trained
Feeding base sterilizes, and is aseptically inoculated with corresponding above-mentioned three kinds of pure bacterium after cooling, wherein training in clostridium butyricum 1 generation strain
It supports base and is inoculated with after clostridium butyricum and be used to completely cut off air, the repeatable benefit of atoleine in media surface one layer of atoleine of covering
With not needing to be passed through inert gas, economical environment-protective；37 DEG C of shaking table cultures for 24 hours, obtain lactobacillus plantarum 1 generation strain liquid, butyric acid shuttle
Bacterium 1 generation strain liquid and saccharomycete 1 generation strain liquid；Wherein lactobacillus plantarum 1 generation bacterium culture medium is MRS culture medium, clostridium butyricum 1
It is RCM culture medium for bacterium culture medium, saccharomycete 1 generation bacterium culture medium is PDA culture medium；
The preparation of (1.2) 2 generation strain liquids: inoculated plant lactobacillus 1 generation strain liquid and yeast on easy expansion culture medium
The inoculum concentration of bacterium 1 generation strain liquid, 1 generation of the lactobacillus plantarum strain liquid is 5%, and the inoculum concentration of saccharomycete 1 generation strain liquid is
1%；1 generation of clostridium butyricum strain liquid, inoculum concentration 0.2% are inoculated in production medium；Preferably, lactobacillus plantarum 1 generation bacterium
Easy expansion culture medium where kind liquid and saccharomycete 1 generation strain liquid is placed in closed container；Clostridium butyricum 1 generation strain liquid list
Rigorous aseptic production is solely carried out in the production medium in fermentor, guarantees the purity of 2 generation of clostridium butyricum bacterium solution；Above-mentioned three kinds
37 DEG C of bacterium solution shaking table cultures obtain lactobacillus plantarum 2 generation strain liquid, clostridium butyricum 2 generation strain liquid and saccharomycete 2 generation strain afterwards for 24 hours
Liquid；
Wherein easy expansion culture medium includes the ingredient of following mass percent: molasses 2%, corn flour 1%, gruel 5%,
Calcium carbonate 0.05%, remaining is water；The easy expansion culture medium cost is lower, and meets needed for lactobacillus plantarum and saccharomycete
Nutriment；The production medium includes the ingredient of following mass percent: dregs of beans 1.5%, fermented bean dregs 0.3%, grape
Sugar 1%, corn flour 1%, sodium chloride 0.03%, calcium carbonate 0.03%, potassium dihydrogen phosphate 0.02%, dipotassium hydrogen phosphate 0.02%,
Ammonium hydrogen carbonate 0.02%, magnesium sulfate 0.02%, manganese sulfate 0.01%, L-cysteine hydrochloride 0.02%, remaining is water, the life
Production culture medium cost is lower, and nutriment is compared with horn of plenty；
(2) black soldier flies larva is placed 2~3 days under field conditions (factors), drains frass in enteron aisle；It is clear by microwave cleaning machine
Black soldier flies larva is broken into worm by meat grinder after washing to starch to obtain Heisui River gadfly slurry, it is preferred that meat grinder discharge port aperture
Lower than 2 millimeters, prevent still having worm living not broken, manufactured worm slurry should timely use, and the resting period must not exceed 12 hours；
(3) in proportion by Heisui River gadfly slurry, corn flour, lactobacillus plantarum 2 generation bacterium solution, clostridium butyricum 2 generation bacterium solution and honey
It places after mixing in a reservoir；
(4) saccharomycete 2 generation bacterium solution is covered on above-mentioned raw materials surface, closed container carries out fermentation 4 days or more, during fermentation
Temperature is controlled at 35~40 DEG C, and i.e. Heisui River gadfly slurry semisolid concentrated feed preparation is completed after fermentation.
Use Heisui River gadfly made of above-mentioned raw materials and method slurry semisolid concentrated feed nutritive index it is as follows: pH value for
4.43, moisture 79.52%, protein 39 .14%, small peptide 34.12%, living bacteria count 5.75*10^8cfu.
Embodiment 2
Substantially with embodiment 1, a kind of Heisui River gadfly slurry semisolid concentrated feed, each group including following mass percent
Point: Heisui River gadfly slurry 87%, lactobacillus plantarum 2 generation bacterium solution 3%, clostridium butyricum 2 generation bacterium solution 3%, saccharomycete 2 generation bacterium solution 2%, jade
Rice flour 2% and molasses 3%；Heisui River gadfly slurry semisolid concentrated feed is made by following steps:
The preparation of (1.1) 1 generation strain liquids: MRS culture medium, RCM culture medium and PDA culture medium are sterilized respectively, cold
But corresponding three kinds of pure bacterium are aseptically inoculated with after, wherein after clostridium butyricum 1 generation bacterium culture medium is inoculated with clostridium butyricum
It covers one layer of atoleine in media surface to be used to completely cut off air, atoleine is reusable, does not need to be passed through indifferent gas
Body, economical environment-protective；37 DEG C of shaking table cultures for 24 hours, obtain lactobacillus plantarum 1 generation strain liquid, clostridium butyricum 1 generation strain liquid and saccharomycete
1 generation strain liquid；
The preparation of (1.2) 2 generation strain liquids: inoculated plant lactobacillus 1 generation strain liquid and yeast on easy expansion culture medium
The inoculum concentration of bacterium 1 generation strain liquid, 1 generation of the lactobacillus plantarum strain liquid is 6%, and the inoculum concentration of saccharomycete 1 generation strain liquid is
2%；1 generation of clostridium butyricum strain liquid, inoculum concentration 0.25% are inoculated in production medium；Preferably, lactobacillus plantarum 1 generation bacterium
Easy expansion culture medium where kind liquid and saccharomycete 1 generation strain liquid is placed in closed container；Clostridium butyricum 1 generation strain liquid list
Rigorous aseptic production is solely carried out in the production medium in fermentor, guarantees the purity of 2 generation of clostridium butyricum bacterium solution；Above-mentioned three kinds
37 DEG C of bacterium solution shaking table cultures obtain lactobacillus plantarum 2 generation strain liquid, clostridium butyricum 2 generation strain liquid and saccharomycete 2 generation strain afterwards for 24 hours
Liquid；
Wherein easy expansion culture medium includes the ingredient of following mass percent: molasses 2.5%, corn flour 1.5%, gruel
6.5%, calcium carbonate 0.075%, remaining is water；The easy expansion culture medium cost is lower, and meets lactobacillus plantarum and yeast
Nutriment needed for bacterium；The production medium includes the ingredient of following mass percent: dregs of beans 1.75%, fermented bean dregs
0.4%, glucose 1.5%, corn flour 1.5%, sodium chloride 0.04%, calcium carbonate 0.04%, potassium dihydrogen phosphate 0.025%, phosphorus
Sour hydrogen dipotassium 0.025%, ammonium hydrogen carbonate 0.025%, magnesium sulfate 0.025%, manganese sulfate 0.015%, L-cysteine hydrochloride
0.025%, remaining is water, and cost is relatively low for the production medium, and nutriment is compared with horn of plenty；
(2) black soldier flies larva is placed 2~3 days under field conditions (factors), drains frass in enteron aisle；It is clear by microwave cleaning machine
Black soldier flies larva is broken into worm by meat grinder after washing to starch to obtain Heisui River gadfly slurry, it is preferred that meat grinder discharge port aperture
Lower than 2 millimeters, prevent still having worm living not broken, manufactured worm slurry should timely use, and the resting period must not exceed 12 hours；
(3) by Heisui River gadfly slurry, corn flour, lactobacillus plantarum 2 generation bacterium solution, clostridium butyricum 2 generation bacterium solution and honey, these are former
Material is placed in a reservoir after mixing in proportion；
(4) saccharomycete 2 generation bacterium solution is covered on above-mentioned raw materials surface, closed container carries out fermentation 4 days or more, during fermentation
Temperature is controlled at 35~40 DEG C, and i.e. Heisui River gadfly slurry semisolid concentrated feed preparation is completed after fermentation.
Use Heisui River gadfly made of above-mentioned raw materials and method slurry semisolid concentrated feed nutritive index it is as follows: pH value for
4.22, moisture 78.47%, albumen 38.88%, small peptide 33.84%, living bacteria count 5.81*10^8cfu.
Embodiment 3
Substantially with embodiment 1, a kind of Heisui River gadfly slurry semisolid concentrated feed, each group including following mass percent
Point: Heisui River gadfly slurry 88%, lactobacillus plantarum 2 generation bacterium solution 4%, clostridium butyricum 2 generation bacterium solution 3%, saccharomycete 2 generation bacterium solution 1%, jade
Rice flour 2% and molasses 2%；Heisui River gadfly slurry semisolid concentrated feed is made by following steps:
The preparation of (1.1) 1 generation strain liquids: MRS culture medium, RCM culture medium and PDA culture medium are sterilized respectively, cold
But corresponding three kinds of pure bacterium are aseptically inoculated with after, wherein after clostridium butyricum 1 generation bacterium culture medium is inoculated with clostridium butyricum
It covers one layer of atoleine in media surface to be used to completely cut off air, atoleine is reusable, does not need to be passed through indifferent gas
Body, economical environment-protective；37 DEG C of shaking table cultures for 24 hours, obtain lactobacillus plantarum 1 generation strain liquid, clostridium butyricum 1 generation strain liquid and saccharomycete
1 generation strain liquid；
The preparation of (1.2) 2 generation strain liquids: inoculated plant lactobacillus 1 generation strain liquid and yeast on easy expansion culture medium
The inoculum concentration of bacterium 1 generation strain liquid, 1 generation of the lactobacillus plantarum strain liquid is 7%, and the inoculum concentration of saccharomycete 1 generation strain liquid is
3%；1 generation of clostridium butyricum strain liquid, inoculum concentration 0.3% are inoculated in production medium；Preferably, lactobacillus plantarum 1 generation bacterium
Easy expansion culture medium where kind liquid and saccharomycete 1 generation strain liquid is placed in closed container；Clostridium butyricum 1 generation strain liquid list
Rigorous aseptic production is solely carried out in the production medium in fermentor, guarantees the purity of 2 generation of clostridium butyricum bacterium solution；Above-mentioned three kinds
37 DEG C of bacterium solution shaking table cultures obtain lactobacillus plantarum 2 generation strain liquid, clostridium butyricum 2 generation strain liquid and saccharomycete 2 generation strain afterwards for 24 hours
Liquid；
Wherein easy expansion culture medium includes the ingredient of following mass percent: molasses 3%, corn flour 2%, gruel 8%,
Calcium carbonate 0.1%, remaining is water；The easy expansion culture medium cost is lower, and meets needed for lactobacillus plantarum and saccharomycete
Nutriment；The production medium includes the ingredient of following mass percent: dregs of beans 2%, fermented bean dregs 0.5%, glucose
2%, corn flour 2%, sodium chloride 0.05%, calcium carbonate 0.05%, potassium dihydrogen phosphate 0.03%, dipotassium hydrogen phosphate 0.3%, carbonic acid
Hydrogen ammonium 0.03%, magnesium sulfate 0.03%, manganese sulfate 0.02%, L-cysteine hydrochloride 0.03%, remaining is water, production training
Supporting base, cost is relatively low, and nutriment is compared with horn of plenty；
(2) black soldier flies larva is placed 2~3 days under field conditions (factors), drains frass in enteron aisle；It is clear by microwave cleaning machine
Black soldier flies larva is broken into worm by meat grinder after washing to starch to obtain Heisui River gadfly slurry, it is preferred that meat grinder discharge port aperture
Lower than 2 millimeters, prevent still having worm living not broken, manufactured worm slurry should timely use, and the resting period must not exceed 12 hours；
(3) by Heisui River gadfly slurry, corn flour, lactobacillus plantarum 2 generation bacterium solution, clostridium butyricum 2 generation bacterium solution and honey, these are former
Material is placed in a reservoir after mixing in proportion；
(4) saccharomycete 2 generation bacterium solution is covered on above-mentioned raw materials surface, closed container carries out fermentation 4 days or more, during fermentation
Temperature is controlled at 35~40 DEG C, and i.e. Heisui River gadfly slurry semisolid concentrated feed preparation is completed after fermentation.
Use Heisui River gadfly made of above-mentioned raw materials and method slurry semisolid concentrated feed nutritive index it is as follows: pH value for
4.28, moisture 77.51%, protein 39 .21%, small peptide 34.03%, living bacteria count 6.15*10^8cfu.
Embodiment 4
Substantially with embodiment 1, a kind of Heisui River gadfly slurry semisolid concentrated feed, each group including following mass percent
Point: Heisui River gadfly slurry 86.5%, lactobacillus plantarum 2 generation bacterium solution 4%, clostridium butyricum 2 generation bacterium solution 3%, saccharomycete 2 generation bacterium solution 2%,
Corn flour 1.5% and molasses 3%；Heisui River gadfly slurry semisolid concentrated feed is made by following steps:
The preparation of (1.1) 1 generation strain liquids: MRS culture medium, RCM culture medium and PDA culture medium are sterilized respectively, cold
But corresponding three kinds of pure bacterium are aseptically inoculated with after, wherein after clostridium butyricum 1 generation bacterium culture medium is inoculated with clostridium butyricum
It covers one layer of atoleine in media surface to be used to completely cut off air, atoleine is reusable, does not need to be passed through indifferent gas
Body, economical environment-protective；37 DEG C of shaking table cultures for 24 hours, obtain lactobacillus plantarum 1 generation strain liquid, clostridium butyricum 1 generation strain liquid and saccharomycete
1 generation strain liquid；
The preparation of (1.2) 2 generation strain liquids: inoculated plant lactobacillus 1 generation strain liquid and yeast on easy expansion culture medium
The inoculum concentration of bacterium 1 generation strain liquid, 1 generation of the lactobacillus plantarum strain liquid is 7%, and the inoculum concentration of saccharomycete 1 generation strain liquid is
3%；1 generation of clostridium butyricum strain liquid, inoculum concentration 0.3% are inoculated in production medium；Preferably, lactobacillus plantarum 1 generation bacterium
Easy expansion culture medium where kind liquid and saccharomycete 1 generation strain liquid is placed in closed container；Clostridium butyricum 1 generation strain liquid list
Rigorous aseptic production is solely carried out in the production medium in fermentor, guarantees the purity of 2 generation of clostridium butyricum bacterium solution；Above-mentioned three kinds
37 DEG C of bacterium solution shaking table cultures obtain lactobacillus plantarum 2 generation strain liquid, clostridium butyricum 2 generation strain liquid and saccharomycete 2 generation strain afterwards for 24 hours
Liquid；
Wherein easy expansion culture medium includes the ingredient of following mass percent: molasses 3%, corn flour 2%, gruel 8%,
Calcium carbonate 0.1%, remaining is water；The easy expansion culture medium cost is lower, and meets needed for lactobacillus plantarum and saccharomycete
Nutriment；The production medium includes the ingredient of following mass percent: dregs of beans 2%, fermented bean dregs 0.5%, glucose
2%, corn flour 2%, sodium chloride 0.05%, calcium carbonate 0.05%, potassium dihydrogen phosphate 0.03%, dipotassium hydrogen phosphate 0.3%, carbonic acid
Hydrogen ammonium 0.03%, magnesium sulfate 0.03%, manganese sulfate 0.02%, L-cysteine hydrochloride 0.03%, remaining is water, production training
Supporting base, cost is relatively low, and nutriment is compared with horn of plenty；
(2) black soldier flies larva is placed 2~3 days under field conditions (factors), drains frass in enteron aisle；It is clear by microwave cleaning machine
Black soldier flies larva is broken into worm by meat grinder after washing to starch to obtain Heisui River gadfly slurry, it is preferred that meat grinder discharge port aperture
Lower than 2 millimeters, prevent still having worm living not broken, manufactured worm slurry should timely use, and the resting period must not exceed 12 hours；
(3) by Heisui River gadfly slurry, corn flour, lactobacillus plantarum 2 generation bacterium solution, clostridium butyricum 2 generation bacterium solution and honey, these are former
Material is placed in a reservoir after mixing in proportion；
(4) saccharomycete 2 generation bacterium solution is covered on above-mentioned raw materials surface, closed container carries out fermentation 4 days or more, during fermentation
Temperature is controlled at 35~40 DEG C, and i.e. Heisui River gadfly slurry semisolid concentrated feed preparation is completed after fermentation.
Use Heisui River gadfly made of above-mentioned raw materials and method slurry semisolid concentrated feed nutritive index it is as follows: pH value for
4.34, moisture 80.61%, albumen 38.75%, small peptide 34.26%, living bacteria count 5.94*10^8cfu.
Embodiment 5
Substantially with embodiment 1, a kind of Heisui River gadfly slurry semisolid concentrated feed, each group including following mass percent
Point: Heisui River gadfly starches 87.5%, lactobacillus plantarum 2 generation bacterium solution 3%, clostridium butyricum 2 generation bacterium solution 3%, saccharomycete 2 generation bacterium solution
1.5%, corn flour 2% and molasses 3%；Heisui River gadfly slurry semisolid concentrated feed is made by following steps:
The preparation of (1.1) 1 generation strain liquids: MRS culture medium, RCM culture medium and PDA culture medium are sterilized respectively, cold
But corresponding three kinds of pure bacterium are aseptically inoculated with after, wherein after clostridium butyricum 1 generation bacterium culture medium is inoculated with clostridium butyricum
It covers one layer of atoleine in media surface to be used to completely cut off air, atoleine is reusable, does not need to be passed through indifferent gas
Body, economical environment-protective；37 DEG C of shaking table cultures for 24 hours, obtain lactobacillus plantarum 1 generation strain liquid, clostridium butyricum 1 generation strain liquid and saccharomycete
1 generation strain liquid；
The preparation of (1.2) 2 generation strain liquids: inoculated plant lactobacillus 1 generation strain liquid and yeast on easy expansion culture medium
The inoculum concentration of bacterium 1 generation strain liquid, 1 generation of the lactobacillus plantarum strain liquid is 5%, and the inoculum concentration of saccharomycete 1 generation strain liquid is
1%；1 generation of clostridium butyricum strain liquid, inoculum concentration 0.2% are inoculated in production medium；Preferably, lactobacillus plantarum 1 generation bacterium
Easy expansion culture medium where kind liquid and saccharomycete 1 generation strain liquid is placed in closed container；Clostridium butyricum 1 generation strain liquid list
Rigorous aseptic production is solely carried out in the production medium in fermentor, guarantees the purity of 2 generation of clostridium butyricum bacterium solution；Above-mentioned three kinds
37 DEG C of bacterium solution shaking table cultures obtain lactobacillus plantarum 2 generation strain liquid, clostridium butyricum 2 generation strain liquid and saccharomycete 2 generation strain afterwards for 24 hours
Liquid；
Wherein easy expansion culture medium includes the ingredient of following mass percent: molasses 2%, corn flour 1%, gruel 5%,
Calcium carbonate 0.05%, remaining is water；The easy expansion culture medium cost is lower, and meets needed for lactobacillus plantarum and saccharomycete
Nutriment；The production medium includes the ingredient of following mass percent: dregs of beans 1.5%, fermented bean dregs 0.3%, grape
Sugar 1%, corn flour 1%, sodium chloride 0.03%, calcium carbonate 0.03%, potassium dihydrogen phosphate 0.02%, dipotassium hydrogen phosphate 0.02%,
Ammonium hydrogen carbonate 0.02%, magnesium sulfate 0.02%, manganese sulfate 0.01%, L-cysteine hydrochloride 0.02%, remaining is water, the life
Production culture medium cost is lower, and nutriment is compared with horn of plenty；
(2) black soldier flies larva is placed 2~3 days under field conditions (factors), drains frass in enteron aisle；It is clear by microwave cleaning machine
Black soldier flies larva is broken into worm by meat grinder after washing to starch to obtain Heisui River gadfly slurry, it is preferred that meat grinder discharge port aperture
Lower than 2 millimeters, prevent still having worm living not broken, manufactured worm slurry should timely use, and the resting period must not exceed 12 hours；
(3) in proportion by Heisui River gadfly slurry, corn flour, lactobacillus plantarum 2 generation bacterium solution, clostridium butyricum 2 generation bacterium solution and honey
It places after mixing in a reservoir；
(4) saccharomycete 2 generation bacterium solution is covered on above-mentioned raw materials surface, closed container carries out fermentation 4 days or more, during fermentation
Temperature is controlled at 35~40 DEG C, and i.e. Heisui River gadfly slurry semisolid concentrated feed preparation is completed after fermentation.
Use Heisui River gadfly made of above-mentioned raw materials and method slurry semisolid concentrated feed nutritive index it is as follows: pH value for
4.32, moisture 79.64%, protein 39 .05%, small peptide 33.89%, living bacteria count 6.35*10^8cfu.
The Heisui River gadfly slurry half prepared it can be seen from above-mentioned multiple embodiments using raw material of the present invention and method
Solid-state concentrated feed produces that acid is more, and PH, albumen, the content of small peptide and living bacteria count are relatively stable, can be used as extensive
The processing scheme of black soldier flies product；And the beneficial microbe in Heisui River gadfly slurry semisolid concentrated feed can promote animal intestinal tract
Absorption efficiency, can directly replace fish meal；Preparation method is simple, and cost is relatively low, so that the purposes of Heisui River gadfly obtains greatly
Extension, market value with higher.
Example of the present invention is only that preferred embodiments of the present invention will be described, not to present inventive concept and
Range is defined, and under the premise of not departing from design philosophy of the present invention, this field engineers and technicians are to technology of the invention
The various changes and improvements that scheme is made should all fall into protection scope of the present invention.
Claims (10)
1. a kind of Heisui River gadfly starches semisolid concentrated feed, it is characterised in that: each component including following mass percent: Heisui River
Gadfly slurry 86~88%, lactobacillus plantarum 2 generation bacterium solution 3~4%, clostridium butyricum 2 generation bacterium solution 3~4%, saccharomycete 2 generation bacterium solution 1~
2%, corn flour 1~2% and molasses 2~3%.
2. a kind of Heisui River gadfly according to claim 1 starches semisolid concentrated feed, it is characterised in that: the plant cream bar
The culture medium of bacterium 2 generation bacterium solution and saccharomycete 2 generation bacterium solution is easy expansion culture medium, and easy expansion culture medium includes following quality hundred
Divide the ingredient of ratio: molasses 2~3%, corn flour 1~2%, gruel 5~8% and calcium carbonate 0.05~0.1%, remaining is water.
3. a kind of Heisui River gadfly according to claim 1 starches semisolid concentrated feed, it is characterised in that: the clostridium butyricum
The culture medium of 2 generation bacterium solutions is production medium, and production medium includes the ingredient of following mass percent: dregs of beans 1.5~2%,
Fermented bean dregs 0.3~0.5%, glucose 1~2%, corn flour 1~2%, sodium chloride 0.03~0.05%, calcium carbonate 0.03~
0.05%, potassium dihydrogen phosphate 0.02~0.03%, dipotassium hydrogen phosphate 0.02~0.03%, ammonium hydrogen carbonate 0.02~0.03%, sulphur
Sour magnesium 0.02~0.03%, manganese sulfate 0.01~0.02% and L-cysteine hydrochloride 0.02~0.03%, remaining is water.
4. a kind of preparation method of Heisui River gadfly slurry semisolid concentrated feed, it is characterised in that: the following steps are included:
(1) lactobacillus plantarum 2 generation bacterium solution, clostridium butyricum 2 generation bacterium solution and saccharomycete 2 generation bacterium solution are prepared；
(2) black soldier flies larva is placed 2~3 days under field conditions (factors), beats black soldier flies larva after being cleaned by microwave cleaning machine
Adult starches to obtain Heisui River gadfly slurry；
(3) Heisui River gadfly slurry, corn flour, lactobacillus plantarum 2 generation bacterium solution, clostridium butyricum 2 generation bacterium solution and honey these raw materials are pressed
Ratio is placed in a reservoir after mixing；
(4) saccharomycete 2 generation bacterium solution is covered on above-mentioned raw materials surface, closed container ferments, after fermentation i.e. black soldier flies
Worm starches the preparation of semisolid concentrated feed and completes.
5. a kind of preparation method of Heisui River gadfly slurry semisolid concentrated feed according to claim 4, it is characterised in that: institute
It states in step (2) and black soldier flies larva is broken into Heisui River gadfly slurry with meat grinder.
6. a kind of preparation method of Heisui River gadfly slurry semisolid concentrated feed according to claim 5, it is characterised in that: twist
Meat machine discharging bore is less than 2mm and the Heisui River gadfly of preparation starches the resting period no more than 12h.
7. a kind of preparation method of Heisui River gadfly slurry semisolid concentrated feed according to claim 4, it is characterised in that: institute
It states and includes the following steps: in step (1)
The preparation of (1.1) 1 generation strain liquids: by lactobacillus plantarum, clostridium butyricum and the corresponding 1 generation bacterium culture medium of saccharomycete
It sterilizes, corresponding strain is aseptically inoculated with after cooling, 37 DEG C of shaking table cultures for 24 hours, obtain lactobacillus plantarum 1 generation bacterium
Kind liquid, clostridium butyricum 1 generation strain liquid and saccharomycete 1 generation strain liquid；
The preparation of (1.2) 2 generation strain liquids: 1 generation of inoculated plant lactobacillus 1 generation strain liquid and saccharomycete on easy expansion culture medium
Strain liquid, is inoculated with 1 generation of clostridium butyricum strain liquid in production medium, and 37 DEG C of shaking table cultures obtain 2 generation of lactobacillus plantarum afterwards for 24 hours
Strain liquid, clostridium butyricum 2 generation strain liquid and saccharomycete 2 generation strain liquid.
8. a kind of preparation method of Heisui River gadfly slurry semisolid concentrated feed according to claim 7, it is characterised in that: institute
Stating lactobacillus plantarum 1 generation bacterium culture medium in step (1.1) is MRS culture medium, and saccharomycete 1 generation bacterium culture medium is PDA culture
Base, clostridium butyricum 1 generation bacterium culture medium are RCM culture medium.
9. a kind of preparation method of Heisui River gadfly slurry semisolid concentrated feed according to claim 7, it is characterised in that: institute
Stating lactobacillus plantarum 1 generation strain liquid inoculum concentration in step (1.2) is 5~7%, and saccharomycete 1 generation strain liquid inoculum concentration is 1~3%,
Clostridium butyricum 1 generation strain liquid inoculum concentration is 0.2%~0.3%.
10. a kind of preparation method of Heisui River gadfly slurry semisolid concentrated feed according to claim 4, it is characterised in that:
Temperature is controlled at 35~40 DEG C during fermentation in the step (4).
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| CN112194520A (en) * | 2020-10-30 | 2021-01-08 | 海口海森元生物科技有限公司 | Black soldier fly compound amino acid fertilizer and preparation method thereof |
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