CN109090043A - A kind of black soldier flies larva cultural method - Google Patents
A kind of black soldier flies larva cultural method Download PDFInfo
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- CN109090043A CN109090043A CN201810854060.8A CN201810854060A CN109090043A CN 109090043 A CN109090043 A CN 109090043A CN 201810854060 A CN201810854060 A CN 201810854060A CN 109090043 A CN109090043 A CN 109090043A
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- 241001481656 Stratiomyidae Species 0.000 title claims abstract description 148
- 238000000034 method Methods 0.000 title claims abstract description 40
- 238000009395 breeding Methods 0.000 claims abstract description 47
- 230000001488 breeding effect Effects 0.000 claims abstract description 47
- 238000000855 fermentation Methods 0.000 claims abstract description 43
- 230000004151 fermentation Effects 0.000 claims abstract description 37
- 241001465754 Metazoa Species 0.000 claims abstract description 25
- 241000193171 Clostridium butyricum Species 0.000 claims abstract description 23
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 19
- 239000003864 humus Substances 0.000 claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 4
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 4
- 241000894006 Bacteria Species 0.000 claims description 54
- 210000003608 fece Anatomy 0.000 claims description 35
- 239000010871 livestock manure Substances 0.000 claims description 29
- 239000003795 chemical substances by application Substances 0.000 claims description 20
- 239000002131 composite material Substances 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims description 14
- 238000000926 separation method Methods 0.000 claims description 12
- 239000002893 slag Substances 0.000 claims description 12
- 241000193755 Bacillus cereus Species 0.000 claims description 8
- 244000063299 Bacillus subtilis Species 0.000 claims description 8
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 8
- 241000316848 Rhodococcus <scale insect> Species 0.000 claims description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 8
- 239000003818 cinder Substances 0.000 claims description 6
- 210000002700 urine Anatomy 0.000 claims description 6
- 241000235342 Saccharomycetes Species 0.000 claims description 4
- 241001655322 Streptomycetales Species 0.000 claims description 4
- 235000014655 lactic acid Nutrition 0.000 claims description 4
- 239000004310 lactic acid Substances 0.000 claims description 4
- 244000144977 poultry Species 0.000 claims description 3
- 241000187654 Nocardia Species 0.000 claims 2
- 238000004904 shortening Methods 0.000 abstract description 2
- 241000282898 Sus scrofa Species 0.000 description 39
- 150000001875 compounds Chemical class 0.000 description 12
- 230000004083 survival effect Effects 0.000 description 12
- 235000019750 Crude protein Nutrition 0.000 description 11
- 239000000463 material Substances 0.000 description 9
- 239000002245 particle Substances 0.000 description 8
- 239000002361 compost Substances 0.000 description 7
- 238000005336 cracking Methods 0.000 description 7
- 239000003895 organic fertilizer Substances 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- 241001503669 Nocardia brevicatena Species 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
- 241000194020 Streptococcus thermophilus Species 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 239000010828 animal waste Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000010813 municipal solid waste Substances 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000019629 palatability Nutrition 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 101100117236 Drosophila melanogaster speck gene Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000709334 Hermetia Species 0.000 description 1
- 108010060231 Insect Proteins Proteins 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000019577 caloric intake Nutrition 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Biomedical Technology (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
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Abstract
The invention discloses a kind of black soldier flies larva cultural method, include the following steps: that S1. configures black soldier flies breeding bait: the fowl and animal excrement after hot fermentation being mixed with wheat bran, humus, brown sugar and clostridium butyricum by following mass ratio: fowl and animal excrement: wheat bran: humus: brown sugar and clostridium butyricum 900~1000:40~60:20~40:4~5:1~3；S2. larva cultivates: accessing 1~2g black soldier flies newly hatched larvae according to every 1kg black soldier flies breeding bait, 25~35 DEG C are cultivated 6~8 days, and sub-sieve obtains black soldier flies mature larva.The method of the invention can promote the growth of black soldier flies larva and ingest, improve the depletion rate of fowl and animal excrement, while shortening black soldier flies larva cultivation cycle, improve black soldier flies protein content, obtained black soldier flies larva can be used as activated feed or protein sources feedstuff, have biggish application prospect.
Description
Technical field
The invention belongs to resources and environmental technology field, more particularly, to a kind of black soldier flies larva cultural method.
Background technique
Black soldier flies, the flat angle stratiomyiid of scientific name speck (Hermetia illucensL.), Diptera Stratiomyidae insect.In the world
Most area is all distributed, and tropical and subtropical region type is especially abundant.Wherein, septic Stratiomyidae insect can take
The animal protein feed for eating fowl and animal excrement and house refuse production high value, to carry out resource utilization.With black soldier flies larva
For, it is food with the organic matter that kitchen garbage, animal wastes, animals and plants corpse etc. rot, and food can efficiently be converted
For idiotrophic substance, while the insect protein feed of high value is produced, and easily feeding management, at low cost, had at present extensive
Utility value.
The cultural method of black soldier flies larva is usually the certain thickness foodstuff on culturing disk middle berth, then will just have been hatched
Black soldier flies larva is linked into foodstuff.The feeding speed of black soldier flies larva is very fast, and growth is rapid, when growing into certain age in days,
The method for generalling use screening separates black soldier flies larva with remaining foodstuff, i.e., in black soldier flies feeding later period food residues ratio
Relatively dry period, black soldier flies larva is collected together with remaining foodstuff, according to black soldier flies larva and remaining foodstuff particle
The suitable sieve mesh of size selection can obtain the black soldier flies larva containing a small amount of impurity by separating excessive or too small serving piece.
The black soldier flies larva separated is dried by forced air drying equipment or other drying equipments, obtains the dry worm of black soldier flies, with
Standby subsequent use.
However, current black soldier flies are raised usually only with single kitchen garbage or animal wastes, although kitchen garbage
Pass through simple pre-treatment with animal wastes, but still remained when raising black soldier flies palatability is bad, utilization rate not
The problems such as high.
Summary of the invention
The technical problem to be solved by the present invention is to overcome above-mentioned the deficiencies in the prior art, it is feeding to provide a kind of black soldier flies larva
Grow method.
Above-mentioned purpose of the invention is to give realization by the following technical programs:
A kind of black soldier flies larva cultural method, includes the following steps:
S1. black soldier flies breeding bait is configured: by the fowl and animal excrement and wheat bran, humus, brown sugar and butyric acid shuttle after hot fermentation
Bacterium is mixed by following mass ratio: fowl and animal excrement: wheat bran: humus: brown sugar and clostridium butyricum 800~1000:40~60:20~
40:4~5:1~3 control water content 60%~70%；
S2. larva cultivates: accessing 1~2g black soldier flies newly hatched larvae, 25~35 DEG C of cultures 6 according to every 1kg black soldier flies breeding bait
~8 days, sub-sieve obtained black soldier flies mature larva.
The black soldier flies breeding bait that the present invention uses is other than mass content is 85%~95% fowl and animal excrement, also containing auxiliary
Material: wheat bran, humus, brown sugar and clostridium butyricum.The addition of these auxiliary materials can promote the growth of black soldier flies larva and ingest, and make
The wear rate and nutrition conversion ratio for obtaining insect improve, and shorten the cultivation cycle of black soldier flies larva.Auxiliary material it is excessive or it is very few not
Conducive to the growth of black soldier flies larva, if auxiliary material or improper, the black soldier flies larva slow growth of auxiliary material selection is not added, worm amount is produced
Decline.And auxiliary material selected by the present invention is the optimum choice obtained after test of many times is groped, so that black soldier flies breeding bait
Gas porosity increases, good permeability, and nutritional ingredient is suitble to the growth of black soldier flies larva, produces the increase of worm amount and protein content is improved.
Therefore, the black soldier flies breeding bait material formula is also within the scope of the present invention.The formula is by following matter
The each component mixing of amount ratio is prepared: fowl and animal excrement: wheat bran: humus: brown sugar and clostridium butyricum 800~1000:40~60:
20~40:4~5:1~3.
Preferably, fowl and animal excrement adds water to 60% or more water content, obtains black soldier flies breeding bait with after auxiliary materials and mixing.It is black
It after the above-mentioned black soldier flies breeding bait of the access of stratiomyiid ovum, is cultivated at 25~35 DEG C, in the process of black soldier flies larva growth
In, culture bait can gradually fermentation calefaction, when cultivating 6~8 days, moisture is reduced to 40% or so, by black soldier flies larva and remaining
Bait collects together, excessive by separating according to the suitable sieve mesh of black soldier flies larva and the selection of remaining bait granular size
Or too small serving piece, the black soldier flies larva containing a small amount of impurity can be obtained, further sub-sieve, it is aging to can be obtained black soldier flies
Larva.
Preferably, the fowl and animal excrement is to be fermented using composite bacteria agent, and the composite bacteria agent is each by following ratio
Strain composition: bacillus subtilis: Rhodococcus sp: clostridium butyricum: saccharomycete: lactic acid bacteria: streptomycete: bacillus cereus is small more
Spore bacterium=2~5:3~8:2~7:1~4:1~6:2~5:1~3:1~4, living bacteria count are 1.0 × 109cfu/mL.It is logical
It crosses and fowl and animal excrement is fermented using the composite bacteria agent that above-mentioned compounding obtains, the content of beneficial bacterium in fowl and animal excrement can be improved,
The food ration and survival rate of black soldier flies are improved, while promoting the growth of black soldier flies larva, further shortens the training of black soldier flies larva
The period is supported, improves and produces worm amount and protein content.
It is highly preferred that the fowl and animal excrement fermentation process is that poultry excreta is carried out melt cinder separation, urine and excrement are obtained
Slag；Then excrement slag water content after control separation is added composite bacteria agent and carries out anaerobic fermentation, control fermentation humidity 60 less than 60%
~65 DEG C, 60~65 DEG C of fermentation temperature, 10~20h of fermentation time.
Preferably, fowl and animal excrement in the black soldier flies breeding bait: wheat bran: humus: brown sugar and clostridium butyricum 1000:
50:30:5:2.
Preferably, the clostridium butyricum be 200,000,000 effective actives/gram.
Preferably, the fowl and animal excrement is to be fermented using composite bacteria agent, and the composite bacteria agent is each by following ratio
Strain composition: bacillus subtilis: Rhodococcus sp: clostridium butyricum: saccharomycete: lactic acid bacteria: streptomycete: bacillus cereus is small more
Spore bacterium=4:5:4:3:3:3:2:3.
Preferably, the cultivation temperature is 30~35 DEG C.
The black soldier flies larva that the method for the invention is prepared can be used as the feed of poultry or aquaculture, therefore on
It states any black soldier flies larva cultural method and is preparing the application in activated feed or protein sources feedstuff also in the present invention
In protection scope.
Compared with prior art, the invention has the following advantages:
The present invention provides a kind of black soldier flies larva cultural method, the method can promote the growth of black soldier flies larva and ingest,
The depletion rate of fowl and animal excrement is improved, while shortening black soldier flies larva cultivation cycle, improves the conversion effect of fowl and animal excrement
Rate improves black soldier flies protein content, and obtained black soldier flies larva can be used as activated feed or protein sources feedstuff, has larger
Application prospect.
Specific embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of
It limits.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus.
Unless stated otherwise, following embodiment agents useful for same and material are commercially available.
Embodiment 1
A kind of black soldier flies larva cultural method, includes the following steps:
1, it configures black soldier flies breeding bait: the swine excrement after natural high-temperature ferments is crushed, controlling its particle size after cracking is 3
~6mm, then 50kg wheat bran, 30kg humus, 5kg brown sugar and 2kg clostridium butyricum are added into the pig manure after every 1000kg fermentation
(200,000,000 effective actives/gram), are sufficiently stirred mixing, and controlling water content is 65% to get to black soldier flies breeding bait.
2, larva cultivates: according to the ratio of every 1kg black soldier flies breeding bait access 2g black soldier flies newly hatched larvae, by black soldier flies
Newly hatched larvae is inoculated into the surface of black soldier flies breeding bait, the bait with a thickness of 5cm, 35 DEG C are used spin vibration sieve after culture 8 days
Sub-sieve is carried out to get to black soldier flies mature larva and the compost after black soldier flies are processed, it is spare.
By the every processing 10kg pig manure of this above method, about 2.0~2.5 kilograms of fresh black soldier flies mature larva can be obtained, produce
6.5~7.5 kilograms of organic fertilizer out, cultivation survival rate are about 90 %, crude protein content 56.2%.
Embodiment 2
A kind of black soldier flies larva cultural method, includes the following steps:
1, it configures black soldier flies breeding bait: the swine excrement after natural high-temperature ferments is crushed, controlling its particle size after cracking is 3
~6mm, then 40kg wheat bran, 20kg humus, 4kg brown sugar and 1kg clostridium butyricum (2 are added into the pig manure after every 800kg fermentation
Hundred million effective actives/gram), mixing is sufficiently stirred, and controlling water content is 65% to get to black soldier flies breeding bait.
2, larva cultivates: according to the ratio of every 1kg black soldier flies breeding bait access 2g black soldier flies newly hatched larvae, by black soldier flies
Newly hatched larvae is inoculated into the surface of black soldier flies breeding bait, the bait with a thickness of 5cm, 35 DEG C are used spin vibration sieve after culture 8 days
Sub-sieve is carried out to get to black soldier flies mature larva and the compost after black soldier flies are processed, it is spare.
By the every processing 10kg pig manure of this above method, about 2.2~2.8 kilograms of fresh black soldier flies mature larva can be obtained, produce
6.5~7.4 kilograms of organic fertilizer out, cultivation survival rate are about 90 %, crude protein content 56.2%.
Embodiment 3
A kind of black soldier flies larva cultural method, includes the following steps:
1, it configures black soldier flies breeding bait: the swine excrement after natural high-temperature ferments is crushed, controlling its particle size after cracking is 3
~6mm, then 60kg wheat bran, 40kg humus, 5kg brown sugar and 3kg clostridium butyricum (2 are added into the pig manure after every 900kg fermentation
Hundred million effective actives/gram), mixing is sufficiently stirred, and controlling water content is 65% to get to black soldier flies breeding bait.
2, larva cultivates: according to the ratio of every 1kg black soldier flies breeding bait access 2g black soldier flies newly hatched larvae, by black soldier flies
Newly hatched larvae is inoculated into the surface of black soldier flies breeding bait, the bait with a thickness of 5cm, 35 DEG C are used spin vibration sieve after culture 8 days
Sub-sieve is carried out to get to black soldier flies mature larva and the compost after black soldier flies are processed, it is spare.
By the every processing 10kg pig manure of this above method, about 2.1~2.6 kilograms of fresh black soldier flies mature larva can be obtained, produce
6.5~7.5 kilograms of organic fertilizer out, cultivation survival rate are about 90 %, crude protein content 55.4%.
Embodiment 4
A kind of black soldier flies larva cultural method, includes the following steps:
1, compound bacteria handles pig manure
The excreta of pig is subjected to melt cinder separation, obtains urine and excrement slag；Excrement slag water content after control separation is pressed less than 60%
The ratio of every 1kg pig manure inoculation 3g compound bacteria, accesses composite bacteria agent into pig manure and carries out anaerobic fermentation；The composite bacteria agent is by such as
Each strain of lower ratio forms: bacillus subtilis: Rhodococcus sp: clostridium butyricum: brewer's yeast: streptococcus thermophilus: white strepto-
Bacterium: bacillus cereus, micropolyspora brevicatena=4:5:4:3:3:3:2:3, living bacteria count are 1.0 × 109cfu/mL；Control
60~65 DEG C of humidity of system fermentation, 60~65 DEG C of fermentation temperature, fermentation time 20h；
2, it configures black soldier flies breeding bait: the swine excrement after compound bacteria hot fermentation being crushed, its particle size after cracking is controlled
For 3~6mm, then addition 50kg wheat bran, 30kg humus, 5kg brown sugar and 2kg butyric acid shuttle into the pig manure after every 1000kg fermentation
Bacterium (200,000,000 effective actives/gram), is sufficiently stirred mixing, and controlling water content is 65% to get to black soldier flies breeding bait.
3, larva cultivates: according to the ratio of every 1kg black soldier flies breeding bait access 2g black soldier flies newly hatched larvae, by black soldier flies
Newly hatched larvae is inoculated into the surface of black soldier flies breeding bait, the bait with a thickness of 5cm, 35 DEG C are used spin vibration sieve after culture 6 days
Sub-sieve is carried out to get to black soldier flies mature larva and the compost after black soldier flies are processed, it is spare.
By the every processing 10kg pig manure of this above method, about 2.6~2.9 kilograms of fresh black soldier flies mature larva can be obtained, produce
6.7~7.5 kilograms of organic fertilizer out, cultivation survival rate are about 100 %, crude protein content 65.8%.
Embodiment 5
A kind of black soldier flies larva cultural method, includes the following steps:
1, compound bacteria handles pig manure
The excreta of pig is subjected to melt cinder separation, obtains urine and excrement slag；Excrement slag water content after control separation is pressed less than 60%
The ratio of every 1kg pig manure inoculation 3g compound bacteria, accesses composite bacteria agent into pig manure and carries out anaerobic fermentation；The composite bacteria agent is by such as
Each strain of lower ratio forms: bacillus subtilis: Rhodococcus sp: clostridium butyricum: brewer's yeast: streptococcus thermophilus: white strepto-
Bacterium: bacillus cereus, micropolyspora brevicatena=3:5:6:2:4:3:2:2, living bacteria count are 1.0 × 109cfu/mL；Control
60~65 DEG C of humidity of system fermentation, 60~65 DEG C of fermentation temperature, fermentation time 20h；
2, it configures black soldier flies breeding bait: the swine excrement after compound bacteria hot fermentation being crushed, its particle size after cracking is controlled
For 3~6mm, then addition 50kg wheat bran, 30kg humus, 5kg brown sugar and 2kg butyric acid shuttle into the pig manure after every 1000kg fermentation
Bacterium (200,000,000 effective actives/gram), is sufficiently stirred mixing, and controlling water content is 65% to get to black soldier flies breeding bait.
3, larva cultivates: according to the ratio of every 1kg black soldier flies breeding bait access 2g black soldier flies newly hatched larvae, by black soldier flies
Newly hatched larvae is inoculated into the surface of black soldier flies breeding bait, the bait with a thickness of 5cm, 35 DEG C are used spin vibration sieve after culture 6 days
Sub-sieve is carried out to get to black soldier flies mature larva and the compost after black soldier flies are processed, it is spare.
By the every processing 10kg pig manure of this above method, about 2.4~2.8 kilograms of fresh black soldier flies mature larva can be obtained, produce
6.5~7.5 kilograms of organic fertilizer out, cultivation survival rate are about 100 %, crude protein content 61.6%.
Embodiment 6
A kind of black soldier flies larva cultural method, includes the following steps:
1, compound bacteria handles pig manure
The excreta of pig is subjected to melt cinder separation, obtains urine and excrement slag；Excrement slag water content after control separation is pressed less than 60%
The ratio of every 1kg pig manure inoculation 3g compound bacteria, accesses composite bacteria agent into pig manure and carries out anaerobic fermentation；The composite bacteria agent is by such as
Each strain of lower ratio forms: bacillus subtilis: Rhodococcus sp: clostridium butyricum: brewer's yeast: streptococcus thermophilus: white strepto-
Bacterium: bacillus cereus, micropolyspora brevicatena=2:8:2:4:1:5:1:4, living bacteria count are 1.0 × 109cfu/mL；Control
60~65 DEG C of humidity of system fermentation, 60~65 DEG C of fermentation temperature, fermentation time 20h；
2, it configures black soldier flies breeding bait: the swine excrement after compound bacteria hot fermentation being crushed, its particle size after cracking is controlled
For 3~6mm, then addition 60kg wheat bran, 40kg humus, 5kg brown sugar and 3kg butyric acid shuttle into the pig manure after every 900kg fermentation
Bacterium (200,000,000 effective actives/gram), is sufficiently stirred mixing, and controlling water content is 65% to get to black soldier flies breeding bait.
3, larva cultivates: according to the ratio of every 1kg black soldier flies breeding bait access 2g black soldier flies newly hatched larvae, by black soldier flies
Newly hatched larvae is inoculated into the surface of black soldier flies breeding bait, the bait with a thickness of 5cm, 35 DEG C are used spin vibration sieve after culture 8 days
Sub-sieve is carried out to get to black soldier flies mature larva and the compost after black soldier flies are processed, it is spare.
By the every processing 10kg pig manure of this above method, about 2.4~2.7 kilograms of fresh black soldier flies mature larva can be obtained, produce
6.5~7.5 kilograms of organic fertilizer out, cultivation survival rate are about 99 %, crude protein content 63.1%.
Embodiment 7
A kind of black soldier flies larva cultural method, includes the following steps:
1, compound bacteria handles pig manure
The excreta of pig is subjected to melt cinder separation, obtains urine and excrement slag；Excrement slag water content after control separation is pressed less than 60%
The ratio of every 1kg pig manure inoculation 3g compound bacteria, accesses composite bacteria agent into pig manure and carries out anaerobic fermentation；The composite bacteria agent is by such as
Each strain of lower ratio forms: bacillus subtilis: Rhodococcus sp: clostridium butyricum: brewer's yeast: streptococcus thermophilus: white strepto-
Bacterium: bacillus cereus, micropolyspora brevicatena=5:3:7:1:6:2:3:1, living bacteria count are 1.0 × 109cfu/mL；Control
60~65 DEG C of humidity of system fermentation, 60~65 DEG C of fermentation temperature, fermentation time 20h；
2, it configures black soldier flies breeding bait: the swine excrement after compound bacteria hot fermentation being crushed, its particle size after cracking is controlled
For 3~6mm, then addition 60kg wheat bran, 40kg humus, 5kg brown sugar and 3kg butyric acid shuttle into the pig manure after every 900kg fermentation
Bacterium (200,000,000 effective actives/gram), is sufficiently stirred mixing, and controlling water content is 65% to get to black soldier flies breeding bait.
3, larva cultivates: according to the ratio of every 1kg black soldier flies breeding bait access 2g black soldier flies newly hatched larvae, by black soldier flies
Newly hatched larvae is inoculated into the surface of black soldier flies breeding bait, the bait with a thickness of 5cm, 35 DEG C are used spin vibration sieve after culture 8 days
Sub-sieve is carried out to get to black soldier flies mature larva and the compost after black soldier flies are processed, it is spare.
By the every processing 10kg pig manure of this above method, about 2.3~2.7 kilograms of fresh black soldier flies mature larva can be obtained, produce
6.5~7.5 kilograms of organic fertilizer out, cultivation survival rate are about 99 %, crude protein content 65.7%.
Comparative example 1
Same as Example 1, only the side of black soldier flies breeding bait is different, and butyric acid shuttle is not contained in the black soldier flies breeding bait
Bacterium causes black soldier flies initial stage nutrition intake inadequate as the result is shown since palatability is poor, and slow growth needs 15 talentes can output
Mature larva causes production worm amount to fall sharply, and producing worm amount is only the 20% of embodiment 1, and survival rate is not high, and only 75%, crude protein contains
Amount is 40.5%.
Comparative example 2
Same as Example 1, only the side of black soldier flies breeding bait is different, and brown sugar, knot are not contained in the black soldier flies breeding bait
Fruit show black soldier flies initial stage caloric intake it is inadequate, slow growth, need 12 talentes can output mature larva, cause produce worm amount reduce
Obviously, producing worm amount is only the 40% of embodiment 1, and survival rate is not high, and only 80%, crude protein content 41.2%.
Comparative example 3
Same as Example 1, only the proportion of black soldier flies breeding bait is different, adds in the pig manure after specially every 1100kg fermentation
30kg wheat bran, 50kg humus, 6kg brown sugar and 5kg clostridium butyricum (200,000,000 effective actives/gram), black soldier flies nutrition as the result is shown is taken the photograph
Take not enough, slow growth, need 16 talentes can output mature larva, lead to produce worm amount and reduce obvious, producing worm amount is only embodiment 1
10%, and survival rate is low, only 60%, crude protein content 40.9%.Supposition may be because trophic structure is unreasonable and rotten
Matter too high levels are grown, tunning is caused to go mouldy, so as to cause black soldier flies ingestion difficulty, influence its growth.
Comparative example 4
Same as Example 1, only the proportion of black soldier flies breeding bait is different, adds in the pig manure after specially every 700kg fermentation
70kg wheat bran, 100kg humus, 3kg brown sugar and 0.5kg clostridium butyricum (200,000,000 effective actives/gram), black soldier flies are sought as the result is shown
Support intake not enough, slow growth, need 15 talentes can output mature larva, lead to produce worm amount and reduce obvious, it is only real for producing worm amount
The 20% of example 1 is applied, and survival rate is low, only 65%, crude protein content 45.2%.
Claims (9)
1. a kind of black soldier flies larva cultural method, which comprises the steps of:
S1. black soldier flies breeding bait is configured: by the fowl and animal excrement and wheat bran, humus, brown sugar and butyric acid shuttle after hot fermentation
Bacterium is mixed by following mass ratio: fowl and animal excrement: wheat bran: humus: brown sugar and clostridium butyricum 800~1000:40~60:20~
40:4~5:1~3 control water content 60%~70%；
S2. larva cultivates: accessing 1~2g black soldier flies newly hatched larvae, 25~35 DEG C of cultures 6 according to every 1kg black soldier flies breeding bait
~8 days, sub-sieve obtained black soldier flies mature larva, spare.
2. the method according to claim 1, wherein the fowl and animal excrement be fermented using composite bacteria agent,
The composite bacteria agent is made of each strain of following ratio: bacillus subtilis: Rhodococcus sp: clostridium butyricum: saccharomycete: lactic acid
Bacterium: streptomycete: bacillus cereus, Micropolyspora=2~5:3~8:2~7:1~4:1~6:2~5:1~3:1~4.
3. according to the method described in claim 2, it is characterized in that, the fowl and animal excrement fermentation process be by poultry excreta into
The separation of row melt cinder, obtains urine and excrement slag；Then excrement slag water content after control separation is added composite bacteria agent and carries out less than 60%
Anaerobic fermentation, 60~65 DEG C of humidity of control fermentation, 60~65 DEG C of fermentation temperature, 10~20h of fermentation time.
4. according to method described in claim 1, which is characterized in that fowl and animal excrement in the black soldier flies breeding bait: wheat bran: humic
Matter: brown sugar and clostridium butyricum 1000:50:30:5:2.
5. according to method described in claim 1, which is characterized in that the clostridium butyricum be 200,000,000 effective actives/gram.
6. according to the method described in claim 2, it is characterized in that, the fowl and animal excrement be fermented using composite bacteria agent,
The composite bacteria agent is made of each strain of following ratio: bacillus subtilis: Rhodococcus sp: clostridium butyricum: saccharomycete: lactic acid
Bacterium: streptomycete: bacillus cereus, Micropolyspora=4:5:4:3:3:3:2:3.
7. the method according to claim 1, wherein the cultivation temperature is 30~35 DEG C.
8. the method according to claim 1, wherein the fowl and animal excrement is pig manure.
9. any one of the claim 1~8 black soldier flies larva cultural method is preparing activated feed or protein sources feedstuff
In application.
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