CN103688909A - Breeding method of selenium enriched black soldier fly larvae - Google Patents
Breeding method of selenium enriched black soldier fly larvae Download PDFInfo
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Abstract
The invention provides a breeding method of selenium enriched black soldier fly larvae. Inorganic selenium or organic selenium is added into feed of the black soldier fly larvae and the concentration of selenium content in the feed of the black soldier fly larvae is controlled at 10 to 80 mg every kg. The selenium is added into the breeding feed of the black soldier fly larvae to obtain the high selenium content of black soldier fly larva products and accordingly the functional value of the black soldier fly larvae is significantly improved. The selenium is combined with protein and polysaccharide organically in the black soldier fly larvae after bioconversion and accordingly the toxic and side effect and the gastrointestinal stimulation of the chemical selenium are eliminated and the selenium can be absorbed and utilized efficiently and securely.
Description
Technical field
The present invention relates to the rich selenium cultural method of a kind of insect, specifically the rich selenium cultural method of a kind of black soldier flies larva.
Background technology
Black soldier flies larva is the comprehensive high-quality protein resource of nutrient component, contain crude protein 42%, fat 35%, be rich in the required amino acid of many animals, and amino acid forms rationally, azelon is few, be easy to digest and assimilate, simultaneously, black soldier flies larva also contains the needed trace element of multiple vital movement, it is premium animal protein feed, also be the delicious fresh food of special economic animal, feed soft-shelled turtle, wood frog, fish, shrimp, eel, swamp eel, various birds, mink etc., growth is obviously accelerated, effect of increasing production is good, it is the good feed addictive of various animals and pet, bait.Fly maggot also contains necessary other nutrient components in sufficient amount human foods, and every nutritive index is all greater than the protide nutriments such as chicken, pork, beef, soft-shelled turtle.
Selenium is a kind of multi-functional life nutritional element; can remove free radical, anti-oxidant, the powerful generation that suppresses lipid peroxide, strengthen immunologic function, protection biological cell membrane, strengthen prostate regulatory function, prevent blood clot, remove cholesterol; there is the effect identical with insulin, can obviously promote ability, the blood circulation of cellular uptake sugar to prevent skin aging, reduce that luring of carcinogen is carcinous, the cancer cell of eliminating vivo mutations is, the division that stops cancer cell and growth etc.Using inorganic selenium as selenium source, making an addition in the daily ration of fowl poultry is a kind of traditional benefit selenium method, still extensive use at present.Because the toxicity of sodium selenite is stronger, bring inconvenience to the producer and user.Organic Selenium has been compared many advantages with inorganic selenium, first its toxicity is less, and good palatability is convenient to be applied in feed; Its secondary pollutant is higher to the availability of Organic Selenium, thus can reduce the addition of dietary Se, thus reduce the pollution to environment, and the retention rate of Organic Selenium in tissue is higher than sodium selenite.Therefore, the developmental research of organic selenium source is very necessary and has realistic meaning.
At present, the cultural method of black soldier flies larva and cultivator have many patents and bibliographical information, but the rich selenium cultivation of black soldier flies larva there is not yet report.The cultivation that the report of this patent is conducive to further to promote black soldier flies larva utilizes prospect, and the scope of organic selenium source feed is expanded in exploitation, increases the economic worth of black soldier flies.
Summary of the invention
Cultural method for a kind of rich selenium black soldier flies larva of need to providing of the deficiencies in the prior art and aquaculture is provided, to improve the selenium content of black soldier flies larva, increases its nutritive value and economic worth.
Technical scheme of the present invention is: in black soldier flies larvae feeds, add Organic Selenium (Se-enriched yeast or Se-rich grain vegetables etc.) or inorganic selenium (sodium selenite, sodium selenate or selenium mineral powder etc.), selenium constituent content in control black soldier flies larvae feeds is at 10 80mg/kg, in black soldier flies larva raising groove, put into the larvae feeds that contains selenium element, inoculative proportion is 1,000 3000 larva/kg larvae feeds.
The cultural method of rich selenium black soldier flies larva of the present invention comprises:
1, in black soldier flies larvae feeds, add Organic Selenium or inorganic selenium, Organic Selenium can be Se-enriched yeast or Se-rich grain vegetables etc., inorganic selenium can be sodium selenite, sodium selenate or selenium mineral powder etc., and the selenium constituent content in control black soldier flies larvae feeds is at 10 80mg/kg;
2, described black soldier flies larva is cultivated in the following manner:
The black soldier flies adult of acquisition is put in 2m*2m*2m gauze, in gauze, place rotten flower, fruit and vegetable or fowl and animal excrement as drawing ovum matrix, keep its humidity 60 90%, above matrix, place the box of laying eggs simultaneously, controlled condition temperature is 20 40 ℃, air humidity is 40 90%, under natural light irradiation condition, stimulates and lays eggs;
It is 20 40 ℃ that collection ovum is on the same day positioned over temperature, air humidity is to hatch in 40 90% incubator, after hatching larva, in larvae cultivation basin, add moist feed to cultivate, described feed can be add Organic Selenium or inorganic selenium water content at 60 90% wheat bran, dregs of beans, rice bran, flower, fruit and vegetable, fresh fowl and animal excrement or changing food waste etc. rot, disposable feeding intake, black soldier flies larva raising density is 1,000 3000 cephalont body/kg feeds; Cultivate to sieve afterwards for 4 20 days and process the black soldier flies mature larva that obtains being rich in Organic Selenium.
The nutrient component of described black soldier flies larva is (take larva dry weight basis): crude protein content is 33.3 42.1%, crude fat 10.1 31.5%, Se content 5.5 37.2mg/kg.
Beneficial effect of the present invention is: owing to adding feed in black soldier flies larva breeding process, be mainly wheat bran, rice bran, fresh pig manure, rot flower, fruit and vegetable or changing food waste, transformed the organic matter of part contaminated environment, be conducive to environmental protection; Added selenium element simultaneously, thereby obtain rich selenium black soldier flies larva, improved significantly the function value of black soldier flies larva, and selenium element is combined and is converted into Biological Selenium with protein, polysaccharide organic in black soldier flies larva body after biological transformation, having eliminated the toxicity of chemical selenium (as sodium selenite) and stomach stimulates, make selenium can be more efficiently, be more safely absorbed and used.
Embodiment
Below the invention will be further described.
The cultural method of rich selenium black soldier flies larva of the present invention, comprising:
Example one, the rich selenium of employing wheat bran are raised
The black soldier flies adult of acquisition is put in 2m*2m*2m gauze, in gauze, place rotten flower, fruit and vegetable or fowl and animal excrement as drawing ovum matrix, keep humidity 60 90%, above matrix, place the box of laying eggs simultaneously, controlled condition temperature is 20 40 ℃, air humidity is 40 90%, under natural light irradiation condition, stimulates and lays eggs.Collect in the incubator that ovum is on the same day positioned over similarity condition and hatch, until 25 days, hatch after larva, in larvae cultivation basin, add moist feed to cultivate.The moist wheat bran of feeds utilized selection water content 60 90% adds 40mg Se-enriched yeast (in selenium element wt) by per kilogram feed simultaneously.Inoculative proportion is 1,000 2000 low instar larvae/kg larvae feeds, and larva raising conversion temperature is controlled at 20 40 ℃, and relative moisture is controlled at 40 90%, cultivates and sieves processing after 4 20 days, results polypide.
Example two adopts the rich selenium of rice bran to raise
The black soldier flies adult of acquisition is put in 2m*2m*2m gauze, in gauze, place rotten flower, fruit and vegetable or fowl and animal excrement as drawing ovum matrix, keep humidity 60 90%, above matrix, place the box of laying eggs simultaneously, controlled condition temperature is 20 40 ℃, air humidity is 40 90%, under natural light irradiation condition, stimulates and lays eggs.Collect in the incubator that ovum is on the same day positioned over similarity condition and hatch, until 25 days, hatch after larva, in larvae cultivation basin, add moist feed to cultivate.The rice bran of feeds utilized selection water content 60 90% adds 40mg Rich selenium ore powders (in selenium element wt) by per kilogram feed simultaneously.Inoculative proportion is 1,000 2000 low instar larvae/kg larvae feeds, and larva raising conversion temperature is controlled at 20 40 ℃, and relative moisture is controlled at 40 90%, cultivates and sieves processing after 4 20 days, results polypide.
Example three adopts the rich selenium of fresh pig manure to raise
The black soldier flies adult of acquisition is put in 2m*2m*2m gauze, in gauze, place rotten flower, fruit and vegetable or fowl and animal excrement as drawing ovum matrix, keep humidity 60 90%, above matrix, place the box of laying eggs simultaneously, controlled condition temperature is 20 40 ℃, air humidity is 40 90%, under natural light irradiation condition, stimulates and lays eggs.Collect in the incubator that ovum is on the same day positioned over similarity condition and hatch, until 25 days, hatch after larva, in larvae cultivation basin, add moist feed to cultivate.The fresh pig manure of feeds utilized selection water content 60 90% adds 40mg sodium selenite (in selenium element wt) by per kilogram feed simultaneously.Inoculative proportion is 1,000 2000 low instar larvae/kg larvae feeds, and larva raising conversion temperature is controlled at 20 40 ℃, and relative moisture is controlled at 40 90%, cultivates and sieves processing after 4 20 days, results polypide.
Example four adopts the rich selenium of changing food waste to raise
The black soldier flies adult of acquisition is put in 2m*2m*2m gauze, in gauze, place rotten flower, fruit and vegetable or fowl and animal excrement as drawing ovum matrix, keep humidity 60 90%, above matrix, place the box of laying eggs simultaneously, controlled condition temperature is 20 40 ℃, air humidity is 40 90%, under natural light irradiation condition, stimulates and lays eggs.Collect in the incubator that ovum is on the same day positioned over similarity condition and hatch, until 25 days, hatch after larva, in larvae cultivation basin, add moist feed to cultivate.The changing food waste of feeds utilized selection water content 60 90% adds 40mg sodium selenite (in selenium element wt) by per kilogram feed simultaneously.Inoculative proportion is 1,000 2000 low instar larvae/kg larvae feeds, and larva raising conversion temperature is controlled at 20 40 ℃, and relative moisture is controlled at 40 90%, cultivates and sieves processing after 4 20 days, results polypide.
Each nutrient component determining of the rich selenium black soldier flies of example five larva
The black soldier flies mature larva that DIFFERENT FEED source is raised carries out main nutrient composition mensuration: the crude protein determining method in polypide adopts crude protein bioassay standard GB/T6433-94 in State Standard of the People's Republic of China's feed; Methods of Ether Extract Analysis in polypide adopts the bioassay standard GB/T6433-2006/ISO6492:1999 of crude fat in State Standard of the People's Republic of China's feed; In polypide, the mensuration of selenium adopts Atomic Fluorescence Spectrometry, concrete processing procedure is: get the larva of drying at 80 ℃, be placed in nitration mixture (nitric acid: perchloric acid=3:1) on low temp. electric hot plate, heat, during suitably add nitric acid until solution is bright green liquid, continue to be heated near doing, after constant volume, measure.Rich each nutrient component of selenium black soldier flies larva sees the following form:
Claims (4)
1. a cultural method for rich selenium black soldier flies larva, is characterized in that:
(1) in black soldier flies larvae feeds, add Organic Selenium or inorganic selenium, the selenium constituent content in control black soldier flies larvae feeds is at 10 80mg/kg;
(2) described black soldier flies larva is cultivated in the following manner:
The black soldier flies adult of acquisition is put in 2m*2m*2m gauze, in gauze, place rotten flower, fruit and vegetable or fowl and animal excrement as drawing ovum matrix, keep its humidity 60 90%, above matrix, place the box of laying eggs simultaneously, controlled condition temperature is 20 40 ℃, air humidity is 40 90%, under natural light irradiation condition, stimulates and lays eggs;
It is 20 40 ℃ that collection ovum is on the same day positioned over temperature, air humidity is to hatch in 40 90% incubator, after hatching larva, adds moist feed to cultivate in larvae cultivation basin, disposable feeding intake, black soldier flies larva raising density is 1,000 3000 cephalont body/kg feeds; Cultivate to sieve afterwards for 4 20 days and process the black soldier flies mature larva that obtains being rich in Organic Selenium.
2. the cultural method of a kind of rich selenium black soldier flies larva according to claim 1, is characterized in that: described feed is water content at 60 90% wheat bran, dregs of beans, rice bran, flower, fruit and vegetable, fresh fowl and animal excrement or changing food waste rot.
3. the cultural method of a kind of rich selenium black soldier flies larva according to claim 1, is characterized in that: the Organic Selenium adding in described feed is Se-enriched yeast or Se-rich grain vegetables.
4. the cultural method of a kind of rich selenium black soldier flies larva according to claim 1, is characterized in that: the inorganic selenium adding in described feed is sodium selenite, sodium selenate or selenium mineral powder.
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Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104396887A (en) * | 2014-10-31 | 2015-03-11 | 南昌大学 | Preparation method of zinc-rich hermetia illucens larvas |
| CN104396888A (en) * | 2014-10-31 | 2015-03-11 | 南昌大学 | Method for treating copper-containing solid organic waste by using hermetia illucens larvae |
| CN105166475A (en) * | 2015-08-26 | 2015-12-23 | 广东省昆虫研究所 | Application of cyanotis arachnoids to prepare feed additive shortening growth period of hermetia illucens L. larva |
| CN105558440A (en) * | 2016-01-26 | 2016-05-11 | 华南农业大学 | Tenebrio molitor and hermetia illucens feed based on organic refuse fermentation residues |
| CN106040701A (en) * | 2016-05-30 | 2016-10-26 | 湖南卢氏环保科技有限公司 | Processing method for livestock and poultry died of illness |
| CN106107018A (en) * | 2016-06-16 | 2016-11-16 | 李迺灿 | A kind of method producing Se-enriched feedstuff additive |
| CN106305631A (en) * | 2016-08-25 | 2017-01-11 | 仇颖超 | Breeding method of high-protein Hermetia illucens pupae |
| CN106472881A (en) * | 2015-08-24 | 2017-03-08 | Cj第制糖株式会社 | For raising fodder compound of black soldier flies and application thereof |
| CN106472846A (en) * | 2015-08-24 | 2017-03-08 | Cj第制糖株式会社 | The animal feed of application black soldier flies larva |
| CN106962291A (en) * | 2017-03-02 | 2017-07-21 | 广东省生物资源应用研究所 | A kind of method for improving the biological selenium-enriched ability of black soldier flies |
| CN107549126A (en) * | 2017-10-27 | 2018-01-09 | 银川保绿特生物技术有限公司 | A kind of method that black soldier flies larva is cultivated using kitchen leftover base-material |
| CN108669383A (en) * | 2018-03-28 | 2018-10-19 | 广州飞禧特生物科技有限公司 | A kind of black soldier flies feed and preparation method thereof |
| CN108719609A (en) * | 2018-05-17 | 2018-11-02 | 华中农业大学 | A kind of Rana nigromaculata feed containing stratiomyiid mature larva and application |
| CN108740554A (en) * | 2018-04-24 | 2018-11-06 | 广州飞禧特生物科技有限公司 | A kind of swamp eel mixed feed improved food conversion ratio, improve health |
| CN109006699A (en) * | 2018-07-13 | 2018-12-18 | 江苏农牧科技职业学院 | The ecological cultivation method of black soldier flies processing pig manure |
| CN109090043A (en) * | 2018-07-30 | 2018-12-28 | 开平市华声生物科技有限公司 | A kind of black soldier flies larva cultural method |
| CN109757440A (en) * | 2019-03-12 | 2019-05-17 | 天津市植物保护研究所 | A kind of method and application using remaining meal rubbish raising black soldier flies larva |
| CN111066723A (en) * | 2019-12-17 | 2020-04-28 | 李路胜 | Circulating breeding method for selenium-rich hermetia illucens and selenium-rich chickens |
| CN111972571A (en) * | 2020-08-24 | 2020-11-24 | 普罗欧生物科技(武汉)有限公司 | Compound solid breeding feed for hermetia illucens and preparation method thereof |
| CN111972568A (en) * | 2020-08-24 | 2020-11-24 | 普罗欧生物科技(武汉)有限公司 | Strontium-rich solid breeding feed for hermetia illucens and preparation method thereof |
| CN113940314A (en) * | 2021-09-30 | 2022-01-18 | 天津农学院 | Method for improving composition of hermetia illucens body PUFAs by using linseed oil and detection method thereof |
| CN114031449A (en) * | 2021-12-15 | 2022-02-11 | 中农新科(苏州)有机循环研究院有限公司 | Selenium-rich bio-organic fertilizer based on kitchen waste and preparation method thereof |
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| CN104396887A (en) * | 2014-10-31 | 2015-03-11 | 南昌大学 | Preparation method of zinc-rich hermetia illucens larvas |
| CN104396888A (en) * | 2014-10-31 | 2015-03-11 | 南昌大学 | Method for treating copper-containing solid organic waste by using hermetia illucens larvae |
| CN106472846B (en) * | 2015-08-24 | 2020-03-20 | Cj第一制糖株式会社 | Animal feed using hermetia illucens larvae |
| KR101878020B1 (en) * | 2015-08-24 | 2018-07-13 | 씨제이제일제당 (주) | Feed composition for blacksoldier fly and use thereof |
| CN106472881A (en) * | 2015-08-24 | 2017-03-08 | Cj第制糖株式会社 | For raising fodder compound of black soldier flies and application thereof |
| CN106472846A (en) * | 2015-08-24 | 2017-03-08 | Cj第制糖株式会社 | The animal feed of application black soldier flies larva |
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| CN105166475A (en) * | 2015-08-26 | 2015-12-23 | 广东省昆虫研究所 | Application of cyanotis arachnoids to prepare feed additive shortening growth period of hermetia illucens L. larva |
| CN105166475B (en) * | 2015-08-26 | 2018-11-23 | 广东省生物资源应用研究所 | Application of the blackspiked lovegrass herb with root in the feed addictive that black soldier flies larva growth cycle is shortened in preparation |
| CN105558440B (en) * | 2016-01-26 | 2020-02-21 | 华南农业大学 | Tenebrio molitor and hermetia illucens feed based on organic garbage fermentation residues |
| CN105558440A (en) * | 2016-01-26 | 2016-05-11 | 华南农业大学 | Tenebrio molitor and hermetia illucens feed based on organic refuse fermentation residues |
| CN106040701A (en) * | 2016-05-30 | 2016-10-26 | 湖南卢氏环保科技有限公司 | Processing method for livestock and poultry died of illness |
| CN106107018A (en) * | 2016-06-16 | 2016-11-16 | 李迺灿 | A kind of method producing Se-enriched feedstuff additive |
| CN106305631A (en) * | 2016-08-25 | 2017-01-11 | 仇颖超 | Breeding method of high-protein Hermetia illucens pupae |
| CN106962291A (en) * | 2017-03-02 | 2017-07-21 | 广东省生物资源应用研究所 | A kind of method for improving the biological selenium-enriched ability of black soldier flies |
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| CN109757440A (en) * | 2019-03-12 | 2019-05-17 | 天津市植物保护研究所 | A kind of method and application using remaining meal rubbish raising black soldier flies larva |
| CN111066723A (en) * | 2019-12-17 | 2020-04-28 | 李路胜 | Circulating breeding method for selenium-rich hermetia illucens and selenium-rich chickens |
| CN111972571A (en) * | 2020-08-24 | 2020-11-24 | 普罗欧生物科技(武汉)有限公司 | Compound solid breeding feed for hermetia illucens and preparation method thereof |
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| CN113940314B (en) * | 2021-09-30 | 2024-01-09 | 天津农学院 | Method for improving composition of black soldier fly body PUFAs by using linseed oil and detection method thereof |
| CN113940314A (en) * | 2021-09-30 | 2022-01-18 | 天津农学院 | Method for improving composition of hermetia illucens body PUFAs by using linseed oil and detection method thereof |
| CN114031449A (en) * | 2021-12-15 | 2022-02-11 | 中农新科(苏州)有机循环研究院有限公司 | Selenium-rich bio-organic fertilizer based on kitchen waste and preparation method thereof |
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Application publication date: 20140402 |