KR20200071427A - Feed composition for the larva of Protaetia brevitarsis comprising barley sprout or breeding method of the larva using the same - Google Patents

Abstract

The present invention relates to a feed composition of a larva of Protaetia brevitarsis seulensis containing sprout barley and a method for breeding a larva of Protaetia brevitarsis seulensis using the same. The present invention relates to the feed composition of a larva of Protaetia brevitarsis seulensis, the composition comprising the sprout barley, and fermented sawdust which ferments a mixture including oak sawdust, wheat bran, saccharide, and an EM fermented liquid. The present invention can produce the larva of the Protaetia brevitarsis seulensis with an improved survival rate by supplying the feed composition to the larva of the Protaetia brevitarsis seulensis. The present invention can reduce an insect producing cost of a farm by shortening a growth period of the larva.

Description

Feed composition for the larva of Protaetia brevitarsis comprising barley sprout or breeding method of the larva using the same}

The present invention relates to a feed composition of a white-spotted flower larvae containing sprouted barley or a method of breeding a white-spotted flower-plained larva using the same, more specifically, the present invention relates to sprouted barley; And oak sawdust, bran, fermented sawdust containing sugars and EM; and to a feed composition of fermented white spotted flower larvae.

As four species, such as brown larvae, double-headed crickets, white spotted flower radish, and longevity beetle larvae, were registered as general food ingredients in 2016, public interest in insects increased, and mass breeding of insects has been carried out nationwide. It is urgent to set standards for safe breeding and management of edible insects so that they can be safely managed from the production stage. To this end, research on the development of food sources dedicated to edible insects containing safe and highly nutritious ingredients is necessary.

White spotted flower radish ( Protaetia brevitarsis seulensis) is called the genus "Manufacturing" or "Slugs" in traditional oriental medicine such as folk and Donguibogam. As a herbivorous insect of ~15㎜, the body is dark copper-colored, glossy, and yellowish-white patterns are scattered. It lives in Korea, China, Japan, and eastern Siberia, and is known to occur once every 1 to 2 years from April to October. Three-star larvae have an average head width of around 4.3 to 4.5 mm, a body length of 25 to 37 mm, the body of the larva is milky white, and yellow short hairs are dense throughout. Compared to the size of the body, the head size is small and the legs are not developed. The flower radishes ( Protaetia spp.) other than white-spotted flowers, such as small-flowered flowers, true-flowered flowers, black-flowered flowers, blue-flowered flowers, full-colored flowers, broad-flowered flowers, wild-flowered flowers, truly broad-flowered flowers, spotted flowers, There are flower ignorance, Manju spotted flower ignorance, and large purple tiger flower ignorance, but each is recognized as a separate insect.

The white-spotted ignorant is an insect that hatches eggs and hatches them, passes through the 1st to 3rd generation, becomes a pupa after the end, and becomes a mature adult after pupae. The development period from 1 to 3 years is about 4 to 5 months. The most important factor affecting the growth rate of young larvae is the breeding environment, which includes factors such as temperature, food, and humidity. 1-year-old grows and molts until about 10 days later and becomes a 2-year-old larva. The body becomes more and more enlarged and after about 20 days, it becomes a third-generation larva after peeling again. Three-year-old larvae eat hard for about 3 to 4 months, grow and prepare to become pupae as they begin to become species. The species can be distinguished by the color of the 3 larvae's larvae.

The white-spotted ignorance is overwintered in the state of mature larvae of the third age, and the adult insects are active during the daytime and feed on mature fruits such as peaches and pears, or juices such as corn and oak trees, and are always clustered. In addition, these caterpillars inhabit in corrosive soils rich in organic matter such as compost and haystacks.

White-spotted flower ignorance has long been used as a herbal medicine for the treatment of liver disease. In addition, it has recently attracted much attention as an insect resource for exploration and development of useful bioactive substances, production of anti-biological substances, and recovery of liver lipid metabolism damaged by excessive alcohol consumption in experiments with mice. This is known, and its usefulness has been confirmed, such as studies of thrombolytic enzymes and liver protection against liver toxicity induced by administration of carbon tetrachloride in rats.

On the other hand, as attention is focused on the medicinal properties of white-spotted flower ignorance, many people try to collect white-spotted flower ignorance in large quantities, but it is difficult to collect natural products. The larvae of white-spotted ignorance mainly inhabit the roofs of thatched houses, but these white-spotted ignorances are becoming extinct due to the roof improvement project of rural houses and the environmental pollution caused by the abuse of pesticides, and now it is hardly found in rural areas. Therefore, although interest in artificial breeding methods of white spotted ignorance is gradually increasing, systematic studies have not yet been carried out to mass-produce white spotted ignorance, and it is useful because there is a limit to satisfy the demand for rapidly increasing white spotted ignorance. Efficient utilization as a resource is difficult.

Barley sprout is a sprout vegetable that is known to contain a large amount of bioactive ingredients, and is emerging as a health functional ingredient. Sprout barley is a young barley shoot of less than 20 cm in length. It is rich in various vitamins, minerals, and proteins. It is also rich in antioxidant enzymes and contains a large amount of vitamin C, vitamin E, and beta-carotene, which are antioxidant vitamins. Efficacy of enhancing blood pressure, anti-inflammatory, antioxidant, anti-allergic, anti-ulcer, and anti-cancer effects by various functional ingredients has been reported.

Accordingly, the present inventors conducted a study on the development of a food source of corrosive edible insects by adding sprout barley, which has been proven to be effective, to the feed composition of white spotted flower larvae, which are bred a lot as edible insects. Thus, the present invention was completed by confirming that the growth promoting effect of the white spotted flower larvae is excellent.

Korean Patent Publication No. 10-2018-0062536 (Invention name: Breeding of white-spotted flower larvae containing citrus by-products and method of breeding white-spotted flower-plained larvae using the same, Applicant: Republic of Korea, Clover Park Co., Ltd. Date: 2018.06.11) Korean Registered Patent No. 10-0937152 (Invention name: Method for preparing larvae and adult feed of white spotted flower mulberry using mulberry, Applicant: Yecheon-gun, Gyeongsangbuk-do (Yecheon-gun Insect Research Institute) and 1 other person, Registration date: 2010.01.08. ) Korean Registered Patent No. 10-1184002 (Invention name: Artificial feed of flower radish, its manufacturing method and breeding method of flower radish using the same, Applicant: Republic of Korea, Date of registration: 2012.09.12.)

An object of the present invention is to provide a feed composition of a white-spotted flower larvae containing a sprout barley and a method for breeding a white-spotted flower-plain larva using the same. More specifically, the object of the present invention is a sprout barley; And oak sawdust, bran, fermented sawdust containing saccharides and EM; to provide a feed composition of fermented white spotted flower larvae.

The present invention relates to a feed composition of a white spotted flower larva larva, comprising a sprout barley.

Fermented sawdust containing oak sawdust, wheat bran, sugars, and EM may be added to the feed composition.

The feed composition is sprout barley; And oak sawdust, bran, fermented sawdust containing sugar and EM; can be prepared by mixing and fermenting.

The sprout barley and fermented sawdust are preferably mixed in a weight ratio of 1:9 to 1:19.

In addition, the present invention relates to a method for feeding white spotted flower ignorance by feeding the feed composition to white spotted flower larvae.

The white spotted flower larvae, the breeding conditions are preferably 25 to 30 ℃ temperature and 65 to 75% humidity.

Hereinafter, the present invention will be described in detail.

The sprout barley used in the present invention is a sprout of barley having 1 to 3 leaves, about 3 to 9 days old after sprouting, and refers to barley in a young cotyledon state before the main leaf comes out. It is preferable to use the sprout barley having a length of 10 to 20 cm. The sprout barley used in the present invention may be used for both undried and dried sprouts.

Oak sawdust used in the present invention may be used any of sawdust, such as oak, oak, oleander, oyster oak. The oak sawdust is preferably a size in the range of 250 mesh to 350 mesh. If the size of oak sawdust is less than 250 mesh, the caterpillars have poor feeding, and if it exceeds 350 mesh, the particles are small, which is undesirable for water retention. Oak sawdust may be used to manufacture dried wood as oak sawdust, but preferably, sawdust (raw sawdust) of raw wood is preferably used.

The wheat bran used in the present invention is the residue left after the flour is prepared, and the inner skin of the wheat is mixed about 12 to 15%. Due to its unique texture, it is often used as a food ingredient, as a raw material such as miso or yeast, and is well digested and used as a feed for livestock. It has little calories but contains a lot of minerals.

The saccharide used in the present invention is preferably one of one or more selected from those consisting of fructose, sugar, honey, and oligosaccharides. It is better to use sugar.

EM used in the present invention may be an EM stock solution or an EM active solution. EM (Effective Microorganism) is an abbreviation of a useful microbial group, and is a complex microbial preparation in which a group of 80 microorganisms, which are beneficial to humans, are cultured in combination among many microorganisms existing in nature. Prototypes constituting EM bacteria are commonly used in food fermentation, such as yeast, lactic acid bacteria, yeast, photosynthetic bacteria, actinomyces, mold fungi, etc. It is a group of microorganisms where microorganisms coexist with each other and lead to the revitalization of nature by producing antioxidant or antioxidant substances and suppressing corruption. Professor Higaderuo of Ryukyu University, the first developer of EM bacteria, named these fermentation microorganisms "useful microorganisms (EM bacteria)." EM (Effective-Microorgarnisms) used in the present invention can be either commercially available EM stock solutions (sprouts) or EM active solutions. The EM active liquid is mixed with 0.1 to 2 parts by weight of saccharides (one or more selected from fructose, sugar, honey, and oligosaccharides) in 100 parts by weight of water, and 5 to 20 parts by weight of EM stock solution in water mixed with the saccharides After mixing, it can be made by incubating in an anaerobic state for 1 to 5 days at a temperature of 29 to 35°C. More preferably, a mixture of 100 parts by weight of water, 1 part by weight of saccharides, and 20 parts by weight of EM stock solution at a temperature of 32°C Can be prepared by culturing for 3 days.

In the feed composition of the present invention, fermented sawdust fermented with a mixture containing oak sawdust, wheat bran, sugars, and EM may be added. The mixture may include 88 to 96% by weight of oak sawdust, 1 to 5% by weight of bran, 1 to 5% by weight of sugars, and 0.5 to 2% by weight of EM.

Sprout barley in the feed composition of the present invention may be included in 5 to 10% by weight. If the feed composition contains less than 5% by weight of sprout barley, the effect of enhancing the growth of white spotted flower larvae may appear slowly, and when the sprout barley exceeds 10% by weight, survival rate is reduced due to an obstacle to the growth of larvae. You may.

In order to prepare the feed composition of the present invention, fermentation may be further performed by mixing fermented sawdust and sprout barley. At this time, the fermentation conditions are preferably maintained at a temperature of 20 to 35°C for 20 to 30 days. When the temperature is less than 20°C, the microorganisms in the EM are not activated, so fermentation may not be performed well, and even if the temperature exceeds 35°C, the fermentation may not be performed well beyond conditions suitable for the growth of microorganisms. In addition, even if the fermentation period of fermented sawdust and sprout barley is less than 20 days, fermentation may not proceed well, and it may be difficult to use the feed composition because it is over-fermented for more than 30 days.

The present invention is a sprout barley; And mixing the fermented sawdust containing oak sawdust, wheat bran, saccharides and EM and feeding the feed composition of the fermented white-spotted flower larvae to the white-spotted flower-plain larvae to provide a method for breeding white-spotted flower ignorance. The breeding conditions of the white spotted flower plain are not particularly limited except for feeding the feed composition of the present invention, but as a specific example, (step 1), the eggs of the white spotted flower plain are spawned, and hatching larvae are hatched from the eggs. Obtaining steps;

(Step 2) supplying the feed composition of the white spotted flower larva larva of the present invention to the hatching larva obtained in step 1 to obtain a seed larva by breeding;

(Step 3) step of obtaining pupa by dissolving the seed larva obtained in step 2;

(Step 4) obtaining an adult by fable the pupa obtained in step 3; And,

(Step 5) inducing the fable adult obtained in step 4 to mate and spawn; may include.

When breeding white spotted flower radish in the present invention, by selecting at least one of ocher and charcoal powder, fill the feed composition of the present invention in a breeding box lined with a height of 0.5 to 3 cm on the bottom to breed white spotted flower plain fabric. Can be.

The feed composition of the white spotted flower larva of the present invention may further include micronutrients such as nutrients, preservatives, preservatives, antibiotics, vitamins, etc., which are commonly used in insect feed. The nutritional preservatives, preservatives, preservatives, antibiotics, micronutrients, etc. can be arbitrarily selected and used in an amount conventionally added to the feed by those skilled in the art.

When the feed composition of the present invention is supplied to the white spotted flower radish, the size and weight increase compared to the white spotted flower radish grown in a natural state, and the larval period decreases. Since the growth of is good, there is an effect capable of supplying high-quality white spotted flower larvae or adult immensely increased in size and weight.

In addition, the feed composition of the present invention can be used as a feed composition of other flower radish insects other than white spotted flower radish.

In the present invention, it is preferable that the temperature is 23 to 27°C as a breeding environment for white-spotted flowers, and the photoperiod is preferably 15 to 17L (light; bright) and 9 to 7D (dark).

In the present invention, the breeding box for breeding white spotted flower radish is kept clean by washing, disinfecting or exchanging it for a certain period of time to prevent larvae from becoming sick, and when moisturizing, put a tissue moistened with sterile water in a container to keep the humidity constant. It is desirable to maintain. At this time, it is preferable to maintain the humidity at 60-70% R.H. The breeding box may be any one used for breeding insect larvae or adults, but the lid has a constant hole (preferably having a hole at intervals of 3 to 5 mm), or a glass or plastic box. It is good to use. The size of the box can also be used of any size, for example, 270 (length) × 400 (horizontal) × 270 (height) (mm).

The feed composition of the present invention can be used both for feeding larvae or adult insects of white spotted radish, but for feeding adult insects, it is also possible to additionally feed the composition for adult mixed feed containing jelly and edible protein. . The composition for mixed feed for adults can be preferably fed 2-3 times a week. The composition for mixed feed for adults may contain 4 to 6 parts by weight of edible protein based on 100 parts by weight of jelly. The jelly is a food obtained by coagulating pectin, gelatin, agar, alginic acid, etc., as a material, and is prepared in a gel state by hardening sugars such as juice or sugar into gelatin and agar, and commercially available ones can also be used. have. As the edible protein, any of commonly used food ingredients such as casein, powdered milk, soy protein, soy flour, soybean meal, rice bran, rice protein, or protein food used as a feed composition can be used.

The present invention relates to a feed composition of a white-spotted flower larvae containing sprout barley, and the composition can be supplied to a white-spotted flower-blinded larva to produce white-spotted flower-blinded larvae with improved size and weight. In addition, by reducing the growth period of each larvae by stage, there is an effect of reducing the insect production cost of the farm.

1 is a photograph showing a comparison of the size of the white spotted flower larva larvae fed the feed composition containing 5% by weight of sprout barley.
Figure 2 is a photograph showing a comparison of the size of the white spotted flower larva larvae fed the feed composition containing 10% by weight of sprout barley.
Figure 3 shows the survival rate of the white spotted flower larvae according to the feed composition of the present invention.
Figure 4 shows the period for each period of the white spotted flower larvae according to the feed composition of the present invention.
5 is a graph showing the analysis of the mineral content of white spotted flower larvae according to the feed composition of the present invention.
6 is a graph showing the analysis of the amino acid content of white spotted flower larvae according to the feed composition of the present invention.

Hereinafter, a preferred embodiment of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to sufficiently convey the spirit of the present invention to those skilled in the art so that the contents introduced herein are thorough and complete.

Example 1. Preparation of feed composition

First, 93 g of oak sawdust, 3 g of wheat bran, 3 g of sugar, and 1 g of EM stock were mixed and fermented at 25° C. for 25 days to prepare fermented sawdust. The fermented sawdust and sprouted barley were mixed to prepare a feed composition, but mixed so that the sprouted barley was 5% by weight and 10% by weight, respectively. The sprout barley was used to have a length of 10 to 20 cm on the 7th day after sprouting.

After mixing, it was further fermented for 30 days in an indoor fermentation plant (25° C.) to prepare a feed composition of white spotted flower larvae.

Comparative Example 1. Preparation of feed composition containing barley

The feed composition was prepared by the method of Example 1, but was prepared using barley powder instead of sprout barley.

The barley powder was prepared by including all the barley ears, leaves and stems of the grown barley and powdering it. The feed composition was prepared by mixing with fermented sawdust so that the barley powder was 5% by weight.

Experimental Example 1. Feeding of feed composition containing sprouted barley against white spotted flower larvae

Freshly hatched white spotted larvae were selected and prepared, and 25 larvae were prepared for each control, experimental group, and comparative group. For the experiment, the caterpillars were fed into the insect breeding buckets one by one, and then fed, but only the fermented sawdust to which nothing was added to the control group was fed. In the experimental group, the sprout barley prepared in Example 1 was 5 wt% or 10 Feed composition containing 5% by weight of feed composition (whole sprout barley 5g and 10g in the total 100g feed composition, respectively, and the rest is fermented sawdust), and in the comparison group, the feed composition containing 5% by weight of barley powder prepared in Comparative Example 1 (Barley powder was 5 g in the total 100 g feed composition, and the rest was fermented sawdust).

The experiment was carried out for a maximum of 17 weeks from the time of incubation (week 0), and during the experiment, 5 ml of tertiary distilled water was sprayed on the sawdust once a week to give proper moisture to the sawdust.

-Breeding conditions: 　 temperature 26~28℃, humidity 50~60%

Experimental Example 2. Confirmation of survival rate of white spotted flower larvae

The experiment of confirming the survival rate of the white spotted flower larvae was performed for 17 weeks from the time of hatching (week 0) and is shown in FIG. 3. Referring to FIG. 3, it was confirmed that all experimental groups including the control (Control) exhibited a survival rate of 90% or more in all groups (5% by weight or 10% by weight of the feed composition containing sprout barley). In addition, although not shown in FIG. 3, the comparison group (the feed composition feed group containing 5% by weight barley powder) also showed a survival rate of 90% or more.

Experimental Example 3. Confirmation of the increase in white spotted flower larvae

The experiment was conducted in the same manner as in Experimental Example 2, and the weight gain of white spotted flower larvae was checked once every 7 days, and the results are shown in Table 1 below.

Breeding period
(week) Control (g) 5% by weight sprout barley
Feed composition (g) 10% by weight sprout barley
Feed composition (g) 5% by weight barley powder
Feed composition (g) 0 0.008±0.001 0.008±0.001 0.008±0.001 0.008±0.001 One 0.021±0.012 0.022±0.014 0.020±0.012 0.021±0.012 2 0.074±0.019 0.092±0.040 0.090±0.040 0.083±0.032 3 0.145±0.075 0.421±0.120 0.512±0.100 0.164±0.064 4 0.417±0.128 0.706±0.179 1.01±0.348 0.445±0.124 5 0.697±0.125 1.731±0.419 2.212±0.295 0.717±0.100 6 1.232±0.313 2.651±0.251 2.964±0.351 1.426±0.274 7 1.737±0.294 2.955±0.240 3.563±0.143 2.082±0.229 8 1.826±0.296 3.450±0.120 3.165±0.316 2.228±0.235 9 2.082±0.229 3.059±0.329 2.894±0.281 2.577±0.218 10 2.228±0.235 2.805±0.154 - 2.675±0.247 11 2.389±0.209 - - 2.853±0.281 12 2.446±0.198 - - 2.949±0.122 13 2.577±0.218 - - 2.972±0.269 14 2.675±0.247 - - 2.830±0.265 15 2.860±0.247 - - 2.803±0.175 16 2.895±0.338 - - - 17 2.804±0.252 - - -

Referring to Table 1, the white spotted flower larva larvae fed the feed composition containing 5% by weight and 10% by weight sprout barley were the highest gains at 3.450g and 3.563g at about 8 weeks and 7 weeks after hatching, respectively. It reached, and it was confirmed that the control (about 16 weeks after hatching, 2.895 g) grew more than 8 weeks faster. In addition, it was confirmed that the comparison group 5% by weight compared to the white spotted flower larvae fed the feed composition containing barley powder was rapidly grown more than 5 weeks.

Experimental Example 4. Confirmation of growth period for each stage of white spotted flower larvae

The growth period for each stage of the white spotted flower larvae of the experiment carried out in Experimental Example 3 was shown in FIG. 4.

Referring to FIG. 4, it was confirmed that the larval period of all auras was shorter in the white spotted flower larvae fed with the feed composition containing 5% by weight and 10% by weight of sprout barley. In particular, the larval period of the 3rd year was less than 25 days, which was shortened by more than 75% compared to the control group. In addition, although not shown in FIG. 4, it was found that the larval period of all the auras was shortened compared to the comparative group, and in particular, the 3rd larval period was shortened by 65% or more compared to the comparative group.

Through the above results, the total larval periods of white spotted flower larvae fed with feed composition containing 5% and 10% by weight sprout barley were averaged 47 days and 45 days, respectively, compared to 2.7 times or more compared to the control group (average 125 days). It was confirmed that it was 2.4 times shorter than the group (110 days on average).

Experimental Example 5. Analysis of body composition of white spotted flower larvae raised with each feed composition

White spotted flower larvae are spotlighted as raw materials that can replace various health functional foods or pharmaceutical compositions. Therefore, it is important to shorten the growth period of the white-spotted flower larvae or increase the larval growth rate, but if it occurs, such as a decrease in the content of various useful components of the white-spotted flower-larvae larvae itself, this food Or is not suitable for use as a pharmaceutical composition.

Therefore, the general crude components, minerals, amino acids and harmful components of the white spotted flower larvae fed with the feed composition including the sprout barley prepared in Example 1 were analyzed. To this end, as a condition of the feed composition prepared in Example 1 and Comparative Example 1, each caterpillar supplied with a food source was fasted for 2 days, so that all the feces in the body were discharged, and then lyophilized and powdered to prepare various components. Analysis.

Experimental Example 5-1. Analysis of the content of general crude components in the larva body according to each feed composition

The analysis of the content of general crude components was measured according to the Association of Official Analytical Chemists (AOAC). Moisture was measured at 105°C under normal pressure heating and drying, ash was 550°C dry ashing, crude fat was measured by Soxhlet method, and crude protein was measured by Kjeldhal method. It is shown in Table 2 below.

division Control (%) 5% by weight sprout barley
Feed composition (g) 10% by weight sprout barley
Feed composition (g) Moisture 71.7 72.44 68.85 Crude protein 13.16 15.88 16.52 Crude fat 0.67 1.01 0.94 Crude fiber 1.58 1.11 2.42 Crude ash 4.43 1.41 1.84 Carbohydrate 8.46 8.15 9.43

Referring to Table 2, as a result of analyzing the general crude component content, the content of crude protein (1.21 times), crude fat (1.51 times), etc. of white-spotted flower larva larvae fed a feed composition containing 5% by weight sprout barley It was higher than that, and the white spotted flower larva larva that supplied the feed composition containing 10% by weight sprout barley was also found to have higher content of crude protein (1.26 times), crude fat (1.40 times), crude fiber (1.53 times) than the control group. .

Experimental Example 5-2. Analysis of mineral content in larva body according to each feed composition

For the inorganic content analysis, a test solution was prepared according to the wet decomposition method, and analyzed by an inductively coupled plasma (ICP) spectrophotometer, and the analysis results are shown in FIG. 5.

Referring to FIG. 5, the content of 5 kinds of inorganic components in the white spotted flower larvae fed with a feed composition containing 5% by weight and 10% by weight of sprout barley increased as a whole compared to the control group, particularly including 5% by weight of sprout barley The content of copper (4.75 times), zinc (1.66 times), and potassium (1.89 times), which are components related to immune function and antioxidant activity, were high in the white spotted larvae fed the feed composition. In addition, in the white-spotted flower larva larvae that supplied a feed composition containing 10% by weight sprout barley, it was confirmed that the content of copper (3.92 times), zinc (1.58 times), and potassium (1.91 times) was higher than that of the control group. It was found that it can be used as a composition for promoting growth of flower larvae.

Experimental Example 5-3. Analysis of amino acid content in larva body according to each feed composition

As for the amino acid content analysis, 6N HCl was first added to the lyophilized powder sample to fill nitrogen and then hydrolyzed at 110° C. for 24 hours using a heating block. The hydrolyzed sample was concentrated with a rotary pressure concentrator to remove the acid, and then used as a citrate buffer. The sample used was measured through an amino acid analyzer and the results are shown in FIG. 6.

Referring to FIG. 6, the total content of 17 amino acids was increased in white spotted larvae larvae fed with a feed composition containing sprouted barley. Especially, it contains sulfur and is an important amino acid for protein structure formation. It was confirmed that the contents of cysteine and methionine, which are known to be involved in the hormonal composition, were higher in the white spotted flower larvae fed the feed composition including the sprout barley than the control group.

In addition, the content of cysteine and methionine increased by 1.46 times and 1.43 times in white-spotted flower larvae fed with a feed composition containing 5% by weight sprout barley, and white-spotted fish fed with a feed composition containing 10% by weight sprout barley It was confirmed that the content of cysteine and methionine in flower larvae increased 1.47 times and 1.46 times.

Experimental Example 5-4. Analysis of harmful components in larvae according to each feed composition

Hazardous component content analysis was carried out in the same manner as the inorganic content analysis method of Experimental Example 5-2, and the analysis results are shown in Table 3 below.

division Control 5% by weight sprout barley
Feed composition 10% by weight sprout barley
Feed composition As Non-detection Non-detection Non-detection CD Non-detection Non-detection Non-detection Pb Non-detection Non-detection Non-detection

Referring to Table 3, no harmful components (arsenic, cadmium, lead) are detected in all experimental groups including the control group, and thus the feed composition of the present invention is identified as a composition harmless to the larvae. You can judge that it is possible.

Overall, the results of the experiment showed that when the feed composition containing 5% by weight and 10% by weight of sprouted barley was fed, the survival rate was similar to that of the control group, but the size and weight of the white spotted flower larvae increased significantly and grew by age It has been shown to have a shortening effect. In addition, as a result of analysis of general crude components, minerals, and amino acids, it was confirmed that the total content of the ingredients was high in the experimental group adding sprout barley, and it was judged that it would be possible to produce white spotted flower larvae with various functions, and also the agricultural utilization of sprout barley It is expected to increase. Therefore, the addition of sprout barley induces the rapid growth of larvae and the production of healthy larvae, so that it can be used as an exclusive food source for white-spotted flower larvae in insect breeding farms, as well as to reduce the cost of breeding, as well as the competitiveness of white-spotted flower-plained farms. Will be able to improve.

Claims (7)

Feed composition of the white spotted flower radish ( Protaetia brevitarsis ) larva, comprising a sprout barley. According to claim 1,
The feed composition of the white spotted flower larvae larvae, characterized in that the addition of fermented sawdust containing oak sawdust, bran, sugar and EM. According to claim 2,
The feed composition is sprout barley; And oak sawdust, bran, fermented sawdust containing saccharides and EM; mixed and further fermented to produce a white spotted flower larvae feed composition. According to claim 3,
Fermentation conditions of the feed composition is 20 to 35 ℃ 20 to 30 days, characterized in that the white spotted flower larvae feed composition. According to claim 3,
Feed composition of the white spotted flower larva larva, characterized in that the sprout barley and fermented sawdust are mixed in a weight ratio of 1:9 to 1:19. Method for breeding white spotted flower ignorance by feeding the feed composition of claim 1 to larvae of white spotted flower larvae. The method of claim 6,
The white spotted flower larvae breeding conditions are 25 to 30 ℃ temperature and 65 to 75% humidity, characterized in that the white spotted flower cultivation method.

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