CN106962291A - A kind of method for improving the biological selenium-enriched ability of black soldier flies - Google Patents
A kind of method for improving the biological selenium-enriched ability of black soldier flies Download PDFInfo
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- CN106962291A CN106962291A CN201710121516.5A CN201710121516A CN106962291A CN 106962291 A CN106962291 A CN 106962291A CN 201710121516 A CN201710121516 A CN 201710121516A CN 106962291 A CN106962291 A CN 106962291A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a kind of method for improving the biological selenium-enriched ability of black soldier flies.It is that black soldier flies ovum is inoculated into feed to be raised, when larva length to 3 instar larvae, feed is selected using the feed containing 3 instar larvae lethal concentration of 50 inorganic seleniums as eliminating, it is inoculated with 3 instar larvaes and is raised in feed to prepupa to eliminating to select, prepupa turns into adult through pupating, sprouting wings, after after Adult worms producting eggs, complete first generation eliminates choosing, repeat the above steps, carry out next generation eliminates choosing, so circulation, which is eliminated, selects at least three from generation to generation.The method of the present invention improves ability of the black soldier flies to Se accumulation bioconversion, solve the current poor bottleneck of black soldier flies selenium-rich, the selenium-rich level for eliminating the black soldier flies of choosing is high, so as to make selenium-enriched food, medicine using selenium-rich black soldier flies as excellent additive, can commercially have good competitiveness, brought new vigor and vitality for China's functional food industry, it is also possible to improve the added value of black soldier flies.
Description
Technical field：
The invention belongs to biological selenium-enriched technical field, and in particular to a kind of method of the biological selenium-enriched ability of raising black soldier flies.
Background technology：
Selenium (Selenium) was a kind of chalcogen nonmetalloid, from 1817, and the Berzelius of Sweden is from sulfuric acid plant
This element of selenium is made in the red powder material of vitriol chamber bottom.There is selenium antioxidation, antitumaous effect, raising human body to exempt from
Epidemic disease ability, prevention of cardiovascular disease, antagonism, can resist the great function such as toxicity of harmful metal elements in human body.If
Human body selenium deficiency, is easy for suffering from Kaschin-Beck disease, Keshan disease, stomach cancer etc.；Selenium, which also has, weakens the effect that aflatoxins triggers liver cancer, suppression
Generation of breast cancer processed etc. is acted on.The problem of current countries in the world all generally existings selenium insufficiency of intake, seriously threatens human body
Health.
Inorganic selenium because of cumulative toxicity, be difficult to be absorbed and the low problem of safety coefficient, unsuitable humans and animals are directly used.
And Organic Selenium toxicity is low, Small side effects, duplication of the virus in life entity can be suppressed, and cell repair can be participated in, excited
It is more notable than inorganic selenium in immune response, it is the reliable selenium source of mankind's secure unique.
Black soldier flies (Hermtia illucens L.), English name Black soldier fly, are Diptera Stratiomyidaes
A kind of insect.Black soldier flies larva and pupa contain abundant protein and several amino acids, and protein content is high, fat is low,
Cholesterol is low, fleshy fiber rational in infrastructure is few, easy absorption and the features such as trace element abundant etc..Black soldier flies have the generation cycle
The characteristics of short, reproduction speed is fast, raising is easy.But, black soldier flies not high to the ability of Se accumulation bioconversion are to utilize Heisui River
Horsefly carries out the bottleneck of selenium-rich.
The content of the invention：
It is an object of the invention to provide a kind of method for improving the biological selenium-enriched ability of black soldier flies.
The method of the raising biological selenium-enriched ability of black soldier flies of the present invention, it is characterised in that comprise the following steps：By black soldier flies
Ovum is inoculated into feed and raised, when larva length to 3 instar larvae, with the feed containing 3 instar larvae lethal concentration of 50 inorganic seleniums
Feed is selected as eliminating, 3 instar larvaes of inoculation are raised to prepupa in feed to eliminating to select, and prepupa treats adult through pupating, sprouting wings as adult
After spawning, complete first generation eliminates choosing, repeats the above steps, and carry out next generation eliminates choosing, so circulates to eliminate and selects extremely
Few 3 generations.
Because black soldier flies 1,2 instar larvaes are to feed containing selenium sensitivity, LC50It is relatively low, it is unfavorable for black soldier flies and eliminates choosing, therefore, this hair
The bright instar larvae of use black soldier flies 3, which is proceeded by, eliminates choosing.
Eliminating for each described generation chooses used the eliminating of 3 age above larvas culture to select feed to be all with first generation
The feed of the inorganic selenium containing the lethal concentration of 50 selects feed as eliminating.
The feed of the described inorganic selenium containing the lethal concentration of 50 is that inorganic selenium is added in feed, is configured to containing various concentrations
The feed of inorganic selenium, chooses the instar larvae of black soldier flies 3 and is placed in 28 DEG C, raises 24 hours in 60%-80%RH greenhouse, obtain inorganic
24 hours LC of selenium50Value, by this LC50The concentration of value as inorganic selenium in feed concentration, be derived from containing the lethal concentration of 50 without
The feed of machine selenium.
Described circulation, which is eliminated, selects at least three to be preferably that circulation is eliminated and selects 3~30 generations from generation to generation.
Described inorganic selenium is preferably sodium selenite and/or sodium selenate.
Described feed is preferably one or more mixtures in peanut press pulp, dregs of beans, kitchen garbage and corn flour.
The water content of the feed of the described inorganic selenium containing the lethal concentration of 50 is preferably 50%~70%.
The present invention has these characteristics of drug resistance, the resistance to the action of a drug using insect, using the method for eliminating choosing, improves black soldier flies to selenium
The ability of enriched biological conversion, solves the current poor bottleneck of black soldier flies selenium-rich, and the selenium-rich level for eliminating the black soldier flies of choosing is high,
So as to make selenium-enriched food, medicine using selenium-rich black soldier flies as excellent additive, can commercially have well competing
Power is striven, is that China's functional food industry brings new vigor and vitality, it is also possible to improve the added value of black soldier flies.
Embodiment：
In order to be better understood from the essence of invention, the technology contents of the present invention are made specifically with reference to embodiment
It is bright, but present disclosure is not so limited, it is impossible to therefore and it is interpreted as the limitation to the scope of the claims of the present invention.
Embodiment 1：
Sodium selenite is added in peanut press pulp and is configured to peanut press pulp containing selenium (water content 70%), the Central Asia of peanut press pulp containing selenium selenium
The concentration of sour sodium is respectively 10,20,40,80,160,320mg/kg, choose the instar larvae of black soldier flies 3 of the same size and be measured,
30 test worms of every group of processing, 3 repetitions, are placed in 28 DEG C, in 60%~80%RH greenhouse, measure the 24h lethal concentrations of 50 (LC50)
For 43.25mg/kg.
Black soldier flies ovum is inoculated into peanut press pulp (water content 70%) and raised, is transferred when larva length to 3 instar larvae
Raised into the peanut press pulp containing selenium (water content 70%) containing 43.25mg/kg sodium selenites to prepupa, prepupa is through pupating, sprouting wings
As adult (complete 1 generation eliminate choosing), after after Adult worms producting eggs, black soldier flies ovum is inoculated into peanut press pulp (water content 70%)
Raised, (the water content of peanut press pulp containing selenium containing 43.25mg/kg sodium selenites is transferred to when larva length to 3 instar larvae
70%) raised in prepupa, prepupa turns into adult (complete 2 generation eliminate choosing) through pupating, sprouting wings, after after Adult worms producting eggs, by
Black soldier flies ovum is inoculated into peanut press pulp (water content 70%) and raised, be transferred to when larva length to 3 instar larvae containing
Raised in the peanut press pulp containing selenium (water content 70%) of 43.25mg/kg sodium selenites to prepupa, prepupa turns into through pupating, sprouting wings
Worm (complete 3 generation eliminate choosing), so circulation, which is eliminated, selects 3 generations, and the rearing conditions for eliminating choosing are 28 DEG C, 60%~80%RH.
To 5 instar larvaes and prepupa progress selenium-rich assay of the 3rd generation, using the measure side of GB5009.93-2010 selenium in food
Method is measured, and is as a result 5 instar larvaes and prepupa (weight in wet base) selenium content is respectively 4.63,5.08mg/kg.
Embodiment 2：
According to method in embodiment 1,24h LC of the instar larvae of black soldier flies 3 to sodium selenite of the 3rd generation is measured50For
47.86mg/kg.Black soldier flies ovum is inoculated into peanut press pulp (water content 70%) and raised, is turned when larva length to 3 instar larvae
It is connected in the peanut press pulp containing selenium (water content 70%) containing 47.86mg/kg sodium selenites and raises to prepupa, prepupa is through pupating, plumage
It is melted into as adult (complete 1 generation eliminate choosing), after after Adult worms producting eggs, carry out next generation eliminates choosing, and such circulate eliminates choosing
To the 20th generation, the rearing conditions for eliminating choosing are 28 DEG C, 60%~80%RH.5 instar larvaes and prepupa of 20th generation are entered
Row selenium-rich assay, is measured using the assay method of GB 5009.93-2010 selenium in food, as a result for 5 instar larvaes and
Prepupa (weight in wet base) selenium content is respectively 51.27,53.48mg/kg.
Choosing is eliminated according to the method described above to the 30th generation, and selenium-rich content is carried out to 5 instar larvaes and prepupa of the 30th generation
Determine, be measured using the assay method of GB 5009.93-2010 selenium in food, be as a result 5 instar larvaes and prepupa (weight in wet base)
Selenium content is respectively 125.31mg/kg, 133.26mg/kg.
Embodiment 3：
Sodium selenate is added in peanut press pulp and is configured to sodium selenate in peanut press pulp containing selenium (water content 50%), peanut press pulp containing selenium
Concentration be respectively 10,20,40,80,160,320mg/kg, choose the instar larvae of black soldier flies 3 of the same size and be measured, every group
30 test worms, 3 repetitions are handled, 28 DEG C is placed in, in 60%~80%RH greenhouse, measures 24h LC50For 49.11mg/kg.
Black soldier flies ovum is inoculated into peanut press pulp (water content 50%) and raised, is transferred when larva length to 3 instar larvae
Raised into the peanut press pulp containing selenium (water content 50%) containing 49.11mg/kg sodium selenates to prepupa, prepupa is through pupating, sprouting wings into
For adult (complete 1 generation eliminate choosing), after after Adult worms producting eggs, carry out next generation eliminates choosing, it is such circulate to eliminate selects 20
From generation to generation, the rearing conditions for eliminating choosing are 28 DEG C, 60%~80%RH.5 instar larvaes and prepupa to the 20th generation carry out selenium-rich and contained
It is fixed to measure, and is measured using the assay method of GB 5009.93-2010 selenium in food, is as a result that 5 instar larvaes and prepupa are (wet
Selenium content is respectively 69.23,72.48mg/kg again).
Choosing is eliminated according to the method described above to the 30th generation, and selenium-rich content is carried out to 5 instar larvaes and prepupa of the 30th generation
Determine, be measured using the assay method of GB 5009.93-2010 selenium in food, be as a result 5 instar larvaes and prepupa (weight in wet base)
Selenium content is respectively 131.78mg/kg, 140.66mg/kg.
Claims (7)
1. a kind of method for improving the biological selenium-enriched ability of black soldier flies, it is characterised in that comprise the following steps：Black soldier flies ovum is inoculated with
Raised into feed, when larva length to 3 instar larvae, using the feed containing 3 instar larvae lethal concentration of 50 inorganic seleniums as eliminating
Select feed, 3 instar larvaes of inoculation are raised to prepupa in feed to eliminating to select, and prepupa treats Adult worms producting eggs through pupating, sprouting wings as adult
Afterwards, complete first generation eliminates choosing, repeats the above steps, and carry out next generation eliminates choosing, it is such circulate to eliminate selects at least three
From generation to generation.
2. according to the method described in claim 1, it is characterised in that eliminating for each described generation chooses 3 age above larvas to train
Supporting used eliminating selects feed to be all to select feed using the feed of the inorganic selenium containing the lethal concentration of 50 of first generation as eliminating.
3. according to the method described in claim 1, it is characterised in that the feed of the inorganic selenium containing the lethal concentration of 50 is to raise
Inorganic selenium is added in material, the feed of the inorganic selenium containing various concentrations is configured to, the instar larvae of black soldier flies 3 is chosen and is placed in 28 DEG C, 60%-
Raised 24 hours in 80%RH greenhouse, obtain 24 hours LC of inorganic selenium50Value, by this LC50The concentration of value is used as nothing in feed
The concentration of machine selenium, is derived from the feed of the inorganic selenium containing the lethal concentration of 50.
4. according to the method described in claim 1, it is characterised in that described circulation, which is eliminated, to be selected at least three to eliminate for circulation from generation to generation to select 3
~30 generations.
5. according to the method described in claim 1, it is characterised in that described inorganic selenium is sodium selenite and/or sodium selenate.
6. according to the method described in claim 1, it is characterised in that described feed is peanut press pulp, dregs of beans, kitchen garbage and jade
One or more mixtures in ground rice.
7. according to the method described in claim 1, it is characterised in that the feed of the inorganic selenium containing the lethal concentration of 50 it is aqueous
Measure as 50%~70%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107821880A (en) * | 2017-11-23 | 2018-03-23 | 山东农业大学 | A kind of production method of selenium-rich drone pupae |
| CN109258576A (en) * | 2018-08-31 | 2019-01-25 | 武汉工程大学 | Method based on black soldier flies collaboration microbial degradation agricultural crop straw |
| CN111066723A (en) * | 2019-12-17 | 2020-04-28 | 李路胜 | Circulating breeding method for selenium-rich hermetia illucens and selenium-rich chickens |
| CN111109206A (en) * | 2020-02-24 | 2020-05-08 | 大连理工大学 | Preparation method of selenium-rich insect protein powder |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823547A (en) * | 2012-08-22 | 2012-12-19 | 福建省农业科学院植物保护研究所 | Drug-resistant neoseiulus amblyseius cucumeris strain |
| CN103688909A (en) * | 2013-12-20 | 2014-04-02 | 江西昌丰由由生物科技有限公司 | Breeding method of selenium enriched black soldier fly larvae |
-
2017
- 2017-03-02 CN CN201710121516.5A patent/CN106962291B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823547A (en) * | 2012-08-22 | 2012-12-19 | 福建省农业科学院植物保护研究所 | Drug-resistant neoseiulus amblyseius cucumeris strain |
| CN103688909A (en) * | 2013-12-20 | 2014-04-02 | 江西昌丰由由生物科技有限公司 | Breeding method of selenium enriched black soldier fly larvae |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107821880A (en) * | 2017-11-23 | 2018-03-23 | 山东农业大学 | A kind of production method of selenium-rich drone pupae |
| CN109258576A (en) * | 2018-08-31 | 2019-01-25 | 武汉工程大学 | Method based on black soldier flies collaboration microbial degradation agricultural crop straw |
| CN111066723A (en) * | 2019-12-17 | 2020-04-28 | 李路胜 | Circulating breeding method for selenium-rich hermetia illucens and selenium-rich chickens |
| CN111109206A (en) * | 2020-02-24 | 2020-05-08 | 大连理工大学 | Preparation method of selenium-rich insect protein powder |
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| CN106962291B (en) | 2020-12-04 |
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Address after: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Applicant after: Institute of zoology, Guangdong Academy of Sciences Address before: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Applicant before: Guangdong Institute of Applied Biological Resources |
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