CN108719609A - A kind of Rana nigromaculata feed containing stratiomyiid mature larva and application - Google Patents
A kind of Rana nigromaculata feed containing stratiomyiid mature larva and application Download PDFInfo
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- CN108719609A CN108719609A CN201810471223.4A CN201810471223A CN108719609A CN 108719609 A CN108719609 A CN 108719609A CN 201810471223 A CN201810471223 A CN 201810471223A CN 108719609 A CN108719609 A CN 108719609A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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Abstract
The invention discloses a kind of the Rana nigromaculata feed containing stratiomyiid mature larva and application, when stratiomyiid mature larva:Man-made feeds are according to 1:Feed about 41.1-42.6% containing crude protein that 1 collocation obtains, crude fat about 17.2-18.9%, lysine about 2.6%, coarse ash about 12.7-15.8%.The present invention substitutes part man-made feeds using stratiomyiid mature larva as live bait, mix into novel Rana nigromaculata feed, complete nutrients matter needed for Rana nigromaculata can be effectively provided under general cultivating condition, it is effectively guaranteed the survival rate and rate of body weight gain of Rana nigromaculata, and rate of body weight gain, specific growth rate and liveness significantly improve.Rana nigromaculata coefficient of condition, muscle crude fat, muscle crude protein content be superior to pure man-made feeds.
Description
Technical field
The invention belongs to aquatic feeds fields, and in particular to a kind of Rana nigromaculata feed containing stratiomyiid mature larva and answer
With using forage feed Rana nigromaculata provided by the invention, can making the Rana nigromaculata of artificial breeding that there is higher rate of body weight gain, specific
Growth rate and feed efficiency.
Background technology
Rana nigromaculata (Pelophylax nigromaculatus), the scientific name blackspot side pleat frog, Anura, Ranidae, the side pleat frog
Belong to, is distributed widely in China except Hainan, Taiwan, the Yunnan provinces and regions Wai Ge, is the common batrachia in field.
Rana nigromaculata is about 7-8 centimetres at the general body of the frog, and about 50-60 grams of weight reaches as high as 100 grams or so, and of the same age is female
Frog individual is more than the male frog.Tadpole period build is loose, and body is in green and with irregular dark small speckle, and tail is thinner and more delicate and tail fin
Prosperity, tail distal tip is round and narrower, and eye is in the back side of head, and eye spacing is wider, and mouth is small, and lips and teeth formula is that horny jaw is moderate, lower lip breast
A prominent row, bicker have anapophysis.It is divided into head, trunk and four limbs three parts at frog body, adult anury, torso portion is direct with head
It is connected, head can not be freely rotated.Rana nigromaculata head is triangular in shape, and long slightly larger than width, mouth is wealthy, kisses blunt circle and slightly point, closely kisses end
There are two nostrils, with nose valve, can arbitrarily be opened and closed to control gas disengaging.The male frog has a pair of of neck side external vocal sac, pipe sound compared with
Greatly, the female frog is without vocal sac, compared with male croaking of a frog sound it is small.Head top both sides are two, have upper palpebra inferior, lower eye danger top to have one layer
Translucent instant embrane, eye is round and dashes forward, and spacing is relatively narrow, and there is round eardrum at eye rear.Trunk short-wide inside has internal organs organ, end
For gonopore, reproduction and excretory function are had both.Adult frog back is bottle green, yellow green or taupe, there is irregular blackspot, abdomen
Portion is white, no spot.There is the light longitudinal ridge line that a width differs among back, by kissing the through anus in end, body dorsal surface top
There is 1 pair of thicker dorsolateral fold, there is the irregular short skin pleat of 4-6 rows between two dorsolateral folds, if if disconnected continuous, it is different in size.Rana nigromaculata four
Limb is made of two forelimbs, two hind legs.Forelimb is shorter, and referring to side has narrow velum, and hind leg is longer, is full web between toe.The male frog first refers to
Base portion is with thumb pad, for being embraced pair with the female frog during breeding.
Rana nigromaculata is as special cultivation animal, and because of its tasty mouthfeel, medicine, food are mended with using, and consumers in general and cultivation are received
The favor at family.Since the huge market demand, raiser mostly used little Chi high-density breedings, a large amount of use manually to raise in recent years
Material.This aquaculture model can make frog shortage activity, and physical function is poor, add the high nutrition of man-made feeds, lead to battalion in frog body
Surplus is supported, Metabolic stress is big, the symptoms such as generally existing fatty liver.So, the batrachia of cultivation is compared with the wild frog, meat and
Nutrition can all be deteriorated, and immunity is relatively low, be easy to suffer from the diseases of some such as " torticollis diseases ", cause largely to lose to raiser.
Invention content
The object of the present invention is to provide a kind of Rana nigromaculata feed containing stratiomyiid mature larva, utilizes the forage feed blackspot
The frog disclosure satisfy that the nutritional need of Rana nigromaculata, improves the rate of body weight gain of Rana nigromaculata and specific growth rate, enhances the liveness of Rana nigromaculata
With the resistivity to disease.
Final object of the present invention is the provision of a kind of Rana nigromaculata feed containing stratiomyiid mature larva in blackspot
Application in frog artificial breeding.
In order to achieve the above object, the present invention takes following technical measures:
A kind of Rana nigromaculata feed containing stratiomyiid mature larva, including batrachia mixed feed and stratiomyiid mature larva, batrachia
The mass ratio of mixed feed and stratiomyiid mature larva is 1:0.5-2, the quality of the stratiomyiid mature larva is in terms of dry weight;
The batrachia mixed feed, component are by weight ratio:
Every kilogram of premix ingredient contains:Lysine 80g;Methionine 18g;Threonine 25g;Tryptophan 2.6g;Biphosphate
Calcium 400g;Antioxidant 1.5g;Compound vitamin 10g;Manganese 16mg;Iodine 0.15mg;Selenium 0.1mg;Cobalt 0.2mg;Iron 2.3mg;Copper
1.1mg;Zinc 2mg, remaining is starch;
The batrachia mixed feed contains crude protein >=40.0g per 100g feeds, crude fat >=3.0g, and lysine >=
1.80g, coarse ash≤17.0g;
The stratiomyiid mature larva contains crude protein 42.1-45.2g, crude fat 31.4- based on 100g dry weights
34.8g, lysine 3.40g, coarse ash 8.3-14.6g.
Above-described feed, preferably:
The mass ratio of batrachia mixed feed and stratiomyiid mature larva is 1:1, the quality of the stratiomyiid mature larva is with dry
Restatement;The batrachia mixed feed, component are by weight ratio:
Every kilogram of premix ingredient contains:Lysine 80g;Methionine 18g;Threonine 25g;Tryptophan 2.6g;Biphosphate
Calcium 400g;Antioxidant 1.5g;Compound vitamin 10g;Manganese 16mg;Iodine 0.15mg;Selenium 0.1mg;Cobalt 0.2mg;Iron 2.3mg;Copper
1.1mg;Zinc 2mg, remaining is starch;
After batrachia mixed feed and the mixing of stratiomyiid mature larva, per 100g feeds, crude protein 41.1-42.6g, crude fat
17.2-18.9g, lysine 2.6g, coarse ash 12.7-15.8g;
In schemes described above, it is preferred that the stratiomyiid is the flat angle Minamata Wuhan strain of speck.
The application of a kind of Rana nigromaculata feed containing stratiomyiid mature larva in Rana nigromaculata is propagated artificially, including the use of this hair
Bright feed directly feeds Rana nigromaculata, or is used to prepare Rana nigromaculata feed.
Above-described application, it is preferred that when directly feeding, be satiated with food feed 2 times (07 daily:00 and 17:00);
The batrachia mixed feed is stored in dry warehouse, is the spherical little particles of diameter 3mm;Stratiomyiid mature larva is trained
It supports in 61cm × 42cm × 12cm plastic casings, polypide is about 12-15mm, uses preceding progress worm material separation;
The Rana nigromaculata is Rana nigromaculata into the frog.
Compared with prior art, the present invention has the following advantages:
First:The present invention substitutes part man-made feeds using stratiomyiid mature larva, and novel Rana nigromaculata feed is made.Stratiomyiid
Breeding is fast, and growth cycle is short, and the stratiomyiid of experiment only needs 3~4d from egg hatching to larva, and reaching mature larva only needs 12d.Water
Horsefly nutritional ingredient is complete, full of nutrition, and Major Nutrient substance is higher than general batrachia mixed feed, can effectively provide Rana nigromaculata institute
The complete nutrients matter needed, and increase the rate of body weight gain of Rana nigromaculata and specific growth rate.
Second:Stratiomyiid aquaculture cost is relatively low, can be obtained using organic wastes such as feces of livestock and poultry, kitchen garbages to cultivate
, it is a kind of very environmentally friendly insect resources.Using stratiomyiid larva substitute part man-made feeds, effectively reduce feed cost and
Aquaculture cost.Meanwhile the nutritive index of Rana nigromaculata is improved, frog crude protein content and crude fat content are above the general frog
Class mixed feed is raised.
Third:It is directly fed using stratiomyiid mature larva as live bait in this experiment, the frog is while preying on live bait, to people
The intake of work feed also relative increase, and the mobility that live bait promotes cultivation Rana nigromaculata is preyed on, make cultivation Rana nigromaculata more
Active, disease resistance is stronger, and meat is closer to wild.The promotion of cultivation Rana nigromaculata meat can also provide wild Rana nigromaculata
Source plays a protective role.
Specific implementation mode
Below in conjunction with specific embodiment and experimental data, the present invention is further illustrated.Institute in the embodiment of the present invention
Technical solution is stated, is conventional techniques if not otherwise specified, if experimental raw used or material are not specified, purchases
From common biotech firm.Stratiomyiid mature larva comes from Chao Tuo ecological agricultures Co., Ltd of Wuhan City hillside laying hen base, is speck
Flat angle Minamata Wuhan strain, is cultivated by chicken manure.
Batrachia mixed feed used in the embodiment of the present invention is:
Every kilogram of premix ingredient contains:Lysine 80g;Methionine 18g;Threonine 25g;Tryptophan 2.6g;Biphosphate
Calcium 400g;Antioxidant 1.5g;Compound vitamin 10g;Manganese 16mg;Iodine 0.15mg;Selenium 0.1mg;Cobalt 0.2mg;Iron 2.3mg;Copper
1.1mg;Zinc 2mg, remaining is starch.
Embodiment 1:
A kind of application of the Rana nigromaculata feed containing stratiomyiid mature larva in Rana nigromaculata is propagated artificially:
Control group feed 1:
Parts by weight of raw materials
Batrachia mixed feed 100
Stratiomyiid mature larva 0 (in terms of dry weight)
Control group feed contains per 100g:Crude protein 40.0g, crude fat 3.0g, lysine 1.80g, coarse ash≤17.0g.
It is the same below.
The outdoor experiment of 1m × 4m is utilized in the Wuhan City sources An Shanfu Rana nigromaculata cultivation base using the feed of the present embodiment
Pool cultivated average weight is the Rana nigromaculata of 25.0g, and each experiment pool contains 1 ㎡, and the waters of deep about 1m cultivates 100 frogs, setting
Three repetitions.
It is satiated with food and feeds 2 times (07 sooner or later daily:00 and 17:00), daily ration, feeding quantity is the 2% of weight, and according to ingesting and grow
Situation is adjusted.The collection last time feeds residual bait before feeding daily, and drying weighs to calculate food ration.
Illumination is lamp, and environment temperature is natural temperature, and terrestrial environment keeps moistening, and a water was added per 2-3 days, protects
It demonstrate,proves the water surface and is not less than 50cm.Culture-cycle is 35d.
Embodiment 2:
A kind of application of the Rana nigromaculata feed containing stratiomyiid mature larva in Rana nigromaculata is propagated artificially:
A kind of Rana nigromaculata feed containing stratiomyiid mature larva:
Ingredients weight parts
Batrachia mixed feed 75
Stratiomyiid mature larva 25 (in terms of dry weight)
Containing stratiomyiid mature larva after above-mentioned batrachia mixed feed is mixed in proportion with stratiomyiid mature larva to get one kind
Rana nigromaculata feed, Rana nigromaculata feed obtained contains per 100g:Crude protein 40.5-41.3, crude fat 10.1-10.95 rely ammonia
Acid 2.20, coarse ash 14.8-16.4.
Mode is raised and fed with embodiment 1.
Embodiment 3:
A kind of application of the Rana nigromaculata feed containing stratiomyiid mature larva in Rana nigromaculata is propagated artificially:
A kind of Rana nigromaculata feed containing stratiomyiid mature larva,:
Ingredients weight parts
Batrachia mixed feed 50
Stratiomyiid mature larva 50 (in terms of dry weight)
Rana nigromaculata feed obtained contains per 100g:Crude protein 41.1-42.6, crude fat 17.2-18.9, lysine 2.6,
Coarse ash 12.7-15.8.
Mode is raised and fed with embodiment 1.
Embodiment 4:
A kind of application of the Rana nigromaculata feed containing stratiomyiid mature larva in Rana nigromaculata is propagated artificially:
A kind of Rana nigromaculata feed containing stratiomyiid worm powder:
Ingredients weight parts
Batrachia mixed feed 25
Stratiomyiid mature larva 75 (in terms of dry weight)
Rana nigromaculata feed obtained contains per 100g:Crude protein 41.6-43.9, crude fat 24.3-26.85, lysine
3.00, coarse ash 10.5-15.2.
Mode is raised and fed with embodiment 1.
Embodiment 5:
A kind of application of the Rana nigromaculata feed containing stratiomyiid mature larva in Rana nigromaculata is propagated artificially:
Control group feed 2:
Ingredients weight parts
Batrachia mixed feed 0
Stratiomyiid mature larva 100 (in terms of dry weight)
Rana nigromaculata feed obtained contains per 100g:Crude protein 42.1-45.2, crude fat 31.4-34.8, lysine 3.4,
Coarse ash 8.3-14.6
Embodiment 6:
Embodiment 1-5 raises result
Rana nigromaculata effect is raised using above-described embodiment feed, sees Tables 1 and 2.
1 Rana nigromaculata feed of table raises Rana nigromaculata growth result
Note:With data line, different letters indicate significant difference (p<0.05), same letter indicates difference not significantly (P>
0.05) following table is same.
As it can be seen from table 1 compared with embodiment one, it is real after replacing part Rana nigromaculata feed using stratiomyiid mature larva
The average growth rate of Rana nigromaculata and average specific growth rate in example two to embodiment five is applied all to be improved.Example three reaches notable water
It is flat,.Stratiomyiid mature larva additive amount is 50.0 in the feed of embodiment three, the rate of body weight gain of the Rana nigromaculata of raising, specific growth rate
It is above embodiment two with coefficient of condition, the Rana nigromaculata of the feed raising of example IV and embodiment five, feed coefficient is minimum, and with
Example one reaches notable compared to effect.
2 Rana nigromaculata feed of table raises Rana nigromaculata nutritive index
From table 2 it can be seen that compared with embodiment one, it is real after replacing part Rana nigromaculata feed using stratiomyiid mature larva
The crude fat for applying Rana nigromaculata in example two to embodiment five is higher than example one, wherein crude fat content highest in embodiment three.Implement
Crude protein is below embodiment one in example two, example IV and embodiment five, and embodiment three is higher than embodiment one.Embodiment three
Stratiomyiid additive amount is 50.0 in feed, and the crude protein of the Rana nigromaculata of raising, crude fat content are above embodiment two, example IV
The Rana nigromaculata raised with the feed of embodiment five, and coarse ash is minimum.
Influence of the 3 stratiomyiid replacement amount of table to Rana nigromaculata frog liveness
Feed actively to come in rear 30min the food dish stratiomyiid children of feeding nearby due to lacking the evaluation criterion of liveness, therefore selecting
The Rana nigromaculata quantity of worm and man-made feeds is used as standard, and 10 or less are low, and 10-20 are medium, and 20 or more are height,
Liveness situation is shown in Table 3.Therefore the active degree of Rana nigromaculata can be promoted by substituting part man-made feeds using stratiomyiid mature larva.
Raising does not find that the disease frog, conjecture stratiomyiid can improve the anti-of the frog by the liveness of the promotion frog during two months
Sick ability.Separately the immunocompetence of the frog may be improved to a certain extent by feeding stratiomyiid.
Stratiomyiid aquaculture cost is low, and mature larva, prepupa nutritional ingredient are complete, full of nutrition, and protein content is high, also contains antibacterial
The substances such as peptide replace part Rana nigromaculata feed using stratiomyiid mature larva, can significantly improve Rana nigromaculata rate of body weight gain, specific growth
Rate and feed efficiency, and the immunity of Rana nigromaculata is improved, enhancing Rana nigromaculata can also improve the resistivity of disease as live bait
The liveness of Rana nigromaculata makes meat more preferably.
It the composite can be widely applied to the artificial breeding field of Rana nigromaculata.
Claims (5)
1. a kind of Rana nigromaculata feed containing stratiomyiid mature larva, including batrachia mixed feed and stratiomyiid mature larva, batrachia are matched
The mass ratio for closing feed and stratiomyiid mature larva is 1:0.5-2, the quality of the stratiomyiid mature larva is in terms of dry weight;
The batrachia mixed feed, component are by weight ratio:
Ingredients weight parts
Fish meal 8-12 kg
Dregs of beans 32-38 kg
Cotton dregs 14-16 kg
Flour 23-27 kg
Rapeseed meal 8-10 kg
Fish oil 0.8-1.2 kg
Salt 0.1-0.3 kg
50% choline chloride 0.08-0.12 kg
Premix 3-5kg
Every kilogram of premix ingredient contains:Lysine 80g;Methionine 18g;Threonine 25g;Tryptophan 2.6g;Calcium dihydrogen phosphate
400g;Antioxidant 1.5g;Compound vitamin 10g;Manganese 16mg;Iodine 0.15mg;Selenium 0.1mg;Cobalt 0.2mg;Iron 2.3mg;Copper
1.1mg;Zinc 2mg, remaining is starch.
2. Rana nigromaculata feed according to claim 1, it is characterised in that:The quality of batrachia mixed feed and stratiomyiid mature larva
Than being 1:1;
The batrachia mixed feed, component are by weight ratio:
Ingredients weight parts
10 kg of fish meal
35 kg of dregs of beans
15.2 kg of cotton dregs
25 kg of flour
9.5 kg of rapeseed meal
1 kg of fish oil
0.2 kg of salt
50% choline chloride, 0.1 kg
4 kg of premix
Every kilogram of premix ingredient contains:Lysine 80g;Methionine 18g;Threonine 25g;Tryptophan 2.6g;Calcium dihydrogen phosphate
400g;Antioxidant 1.5g;Compound vitamin 10g;Manganese 16mg;Iodine 0.15mg;Selenium 0.1mg;Cobalt 0.2mg;Iron 2.3mg;Copper
1.1mg;Zinc 2mg, remaining is starch.
3. Rana nigromaculata feed according to claim 1, it is characterised in that:The stratiomyiid is speck flat angle stratiomyiid Wuhan product
System.
4. application of the Rana nigromaculata feed described in claim 1 in preparing feed.
5. application of the Rana nigromaculata feed described in claim 1 in being propagated artificially in Rana nigromaculata.
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