CN102228014A - Domestication and culture method and application of hermitia illucens WH - Google Patents
Domestication and culture method and application of hermitia illucens WH Download PDFInfo
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Abstract
The invention relates to the technical field of field acquisition, artificial domestication and mass propagation of insects and in particular discloses a domestication and culture method and an application of hermitia illucens WH. The eggs and adults of Wuhan wild hermitia illucens are acquired and respectively raised and domesticated, thus obtaining the stable artificial propagation strain and creating a technology of mass raising. The hermitia illucens WH larvae raised in mass are utilized to transform pig manures, chicken manures and cattle manures respectively and accumulation of livestock and poultry manures can be reduced by more than 50% through treatment. The obtained prepupa contains 35-39% of crude protein, the protein is formed by 17 amino acids, the prepupa contains 33-35% of fat and the fat can serve as the high-quality feed ingredient and nutrition. The domestication and culture method has the following advantages and beneficial effects: the hermitia illucens larvae are utilized to transform livestock and poultry manures; the method has the advantages of simpleness and convenience in operation, deodorization, pathogenic bacteria removal, zero energy consumption, easiness in popularization and the like; and the environmental pollutants are effectively utilized and the high added value product can be obtained.
Description
Technical field
The present invention relates to field acquisition, domestication and a large amount of propagation technique field of insect, be specifically related to the domesticating and cultivating method and the application of stratiomyiid Wuhan, the flat angle of a kind of speck strain.
Background technology
1, the flat angle of speck stratiomyiid
The flat angle of speck stratiomyiid (Hermitia illucens) is a kind of insect of diptera Stratiomyidae.This build is similar to the savanna that the black insect of the gadfly originates from South America, and mainly ight soil and the corpse with animal is food.The flat angle of speck stratiomyiid all has distribution in the most of area in the torrid zone and temperate zone in the whole world, and is also very common in China.Kind surplus China known 170 in Beijing, all there is record in area such as Hebei, Fujian, Guangdong, Guangxi, Hainan, Hubei, generally occurs in May to the November in every year.
2, feces of livestock and poultry
Adjustment along with the structure of agricultural production, the tradition aquaculture transforms to novel intensive culture, the feces of livestock and poultry discharge capacity is increasing, it is estimated, the discharge capacity of China's feces of livestock and poultry in 2010 will be up to 4,500,000,000 tons, become one of very outstanding environomental pollution source, therefore, the harmless treatment of feces of livestock and poultry is paid close attention to day by day.The conventional method that feces of livestock and poultry is handled mainly contains physics method, chemical method and bioanalysis three major types.Physics method and chemical Treatment time consumption and energy consumption, poor processing effect, and produce secondary pollution; Bioanalysis is handled several methods such as lonely pond, good gas oxidation pond and compost, have that cost is low, the tunning biologically active is strong, fertilizer efficiency is high, be easy to advantages such as popularization, be considered to handle feces of livestock and poultry method preferably at present, but, bioanalysis exist long, product of transformation period can not be high-valued etc. problem.
3, insect source protein
Along with the high speed development of World Economics, the mankind have faced various crises of resource, and wherein the protein resource shortage has been current ubiquitous problem.China's protein resource shortage problem is especially serious, and China's protein feeds breach reached 3,800 ten thousand tons in 2010, according to one's analysis, will reach 4,800 ten thousand tons to the year two thousand twenty breach.Insect is a biological group maximum on the earth, studies show that, it is fast, short from generation to generation that insect has growth and breeding, raising is convenient, cost is low, advantages such as polypide is nutritious, rational in infrastructure can obtain a large amount of insect polypides in a short time, are considered to the animal protein and the new fats source of potentialization.Contain albumen 33--34% in the prepupa of stratiomyiid Wuhan, the flat angle of speck strain, fatty 33--35% is good insect protein source.
Stratiomyiid Wuhan, the flat angle of speck strain is cultured in a large number, be used for converting animal-poultry faeces, and do not appear in the newspapers as yet simultaneously as the research in insect protein source.
Summary of the invention
At the deficiencies in the prior art, the domesticating and cultivating method and the application of stratiomyiid Wuhan, the flat angle of a kind of speck strain have been the object of the present invention is to provide.Near the chicken farm, trap a kind of insect of energy converting animal-poultry faeces, eating habit characteristics according to this insect, developing a cover utilizes insect to handle the conversion process of feces of livestock and poultry, thereby fully solve the problem of environmental pollution that the ight soil accumulation of current plant causes, turn waste into wealth simultaneously, change into high-quality substituted type animal protein feed, for later practical application and theoretical research lay the first stone.
To achieve these goals, the present invention has taked following technical measures:
The domesticating and cultivating method of stratiomyiid Wuhan, the flat angle of a kind of speck strain, its step is as follows:
1, the outdoor collection that lures of wild stratiomyiid
Near the accumulation of suburbs, Wuhan has the place of chicken manure or pig manure or cow dung, fill chicken manure (having common house-fly grub to get chicken manure the best of food) with plastic basin, use the branch proping, the plastic basin of filling chicken manure is placed on the support, avoid the interference of carnivorous insects such as ant, lure the flat angle of speck stratiomyiid adult to lay eggs, the hatching back is collected larva and is taken back in the laboratory;
Or near the accumulation of suburbs, Wuhan has the place of chicken manure or pig manure or cow dung, fill chicken manure (having common house-fly grub to get chicken manure the best of food) with plastic basin, on plastic basin, overlap a plastic wire then, lure the flat angle of speck stratiomyiid adult to get food, the adult that captures taken back the laboratory and place the worm cage of 40cm * 40cm * 120cm, make its mate and oviposit.
2, the indoor domestication of wild stratiomyiid
With the larva of field acquisition and/or the larva that lures integrated worm to lay eggs to be hatched (wheat bran, alfalfa meal, corn flour add the water preparation after mixing with 5: 3: 2 weight ratio with artificial feed, make water content reach 70---80%, mass percent) at room temperature 24-30 ℃, carry out artificial feeding under the humidity 75--80%, selecting healthy and strong larva raising from the larva that survives puts in the worm cage of illumination after pupating, turn into adult,, guarantee that humidity is at 75--80% regularly toward worm cage water spray; On overcast and rainy daytime, with iodine-tungsten lamp (500W, 135 μ molm
-2S
-1) mating of irradiation stimulation adult, adult post-coitum self-sow is to laying eggs, at natural lighting or iodine-tungsten lamp (500W, 135 μ molm
-2S
-1) shine and collect worm's ovum (worm's ovum of collection can both normally be hatched) down, the raising that uses the same method of the larva of ovum hatching, domestication was raised more than 10 generations so repeatedly, obtain the larva bodily form, size unanimity, the colony of the adult bodily form, stable performance of the same size identifies, by the morphological feature to female insect and male insect, and the evaluation of larva form, be defined as the flat angle of stratiomyiid subfamily Hermetia speck, the flat angle of Stratiomyidae stratiomyiid Wuhan strain WH (Hermitia illucens WH).
The flat angle of the speck stratiomyiid Wuhan strain WH that above-mentioned domesticating and cultivating method obtains can be used for converting animal-poultry faeces to reduce the feces of livestock and poultry accumulation.Concrete application method is as follows:
The flat angle of speck stratiomyiid Wuhan strain WH is inserted in the feces of livestock and poultry according to the ratio larva of 1000/kg, when raising 40% larva and beginning to pupate, can reduce the ight soil cumulant more than 50%, the transformation efficiency height, and tangible deodorizing and fungistatic effect have been arranged, thereby reduced environmental pollution and effectively utilized waste material; And the prepupa of acquisition contains crude protein 35--39%, contains 17 seed amino acids in the protein, the fatty 33--35% of prepupa, fat is made up of abundant unsaturated fatty acid, has very high nutritive value, can be used as nutrient and healthcare products or animal protein feed after collection and processing are handled.
Compared with prior art, advantage of the present invention and beneficial effect are as follows:
(1) stratiomyiid Wuhan, the flat angle of speck strain polypide is bigger, has improved the insect protein yield.
(2) it is better that stratiomyiid Wuhan, the flat angle of speck strain is handled pig manure, chicken manure, cow dung effect.
(3) stratiomyiid Wuhan, the flat angle of speck strain larvae protein content is higher.
(4) amount of total fat of stratiomyiid Wuhan, the flat angle of speck strain larva is than the flat angle of the speck of other strains stratiomyiid larva height.
(5) stratiomyiid Wuhan, the flat angle of speck strain adapts to local weather conditions, than the easier raising of the flat angle of the speck of other strains stratiomyiid.
Embodiment
Below in conjunction with specific embodiment the inventive method is done further and to be elaborated.
Embodiment 1: luring of the flat angle of wild speck stratiomyiid collects and domestication
1, the outdoor collection that lures of wild stratiomyiid
Near near suburbs, Wuhan (South Lake, Hongshan District, Wuhan City) accumulation has the place of chicken manure or pig manure or cow dung, fill chicken manure (having common house-fly grub to get chicken manure the best of food) with plastic basin, use the branch proping, the plastic basin of filling chicken manure is placed on the support, avoid the interference of carnivorous insects such as ant, lure the flat angle of speck stratiomyiid adult to lay eggs, the hatching back is collected larva and is taken back in the laboratory;
Or have in accumulation near the place of chicken manure or pig manure or cow dung, fill chicken manure (having common house-fly grub to get chicken manure the best of food) with plastic basin, on plastic basin, overlap a plastic wire then, lure the flat angle of speck stratiomyiid adult to get food, the adult that captures taken back the laboratory and place the worm cage of 40cm * 40cm * 120cm, make its mate and oviposit.
2, the indoor domestication of wild stratiomyiid
With the larva of field acquisition and/or the larva that lures integrated worm to lay eggs to be hatched (add the water preparation after mixing with 5: 3: 2 weight ratio by wheat bran, alfalfa meal, corn flour with artificial feed, make water content reach 70--80%) at room temperature 24-30 ℃, humidity 75--80%) carries out artificial feeding under, selecting healthy and strong larva raising from the larva that survives puts in the worm cage of illumination after pupating, turn into adult, regularly, guarantee that humidity is at 75--80% toward worm cage water spray.On overcast and rainy daytime, with iodine-tungsten lamp (500W, 135 μ molm
-2S
-1) mating of irradiation stimulation adult, adult post-coitum self-sow is to laying eggs, at natural lighting or iodine-tungsten lamp (500W, 135 μ molm
-2S
-1) shine and collect worm's ovum (worm's ovum of collection can both normally be hatched) down, the raising that uses the same method of the larva of ovum hatching, domestication was raised more than 10 generations so repeatedly, obtain the larva bodily form, size unanimity, the colony of the adult bodily form, stable performance of the same size identifies, by the morphological feature to female insect and male insect, and the evaluation of larva form, be defined as the flat angle of stratiomyiid subfamily Hermetia speck, the flat angle of Stratiomyidae stratiomyiid Wuhan strain WH (Hermitia illucens WH).
To be used for following examples through the flat angle of the speck stratiomyiid Wuhan strain WH after identifying, to study its performance and application.
Embodiment 2: the biological property of stratiomyiid Wuhan, the flat angle of speck strain reaches the comparison with Guangzhou strain and Dallas's strain
1, the ovum phase
Respectively get 30 pieces of an egg of identical time product in 3 strains, be placed on respectively in three Cans, cover wet gauze, write down each pieces of an egg brooding time, the average brooding time of 30 pieces of an egg is the ovum phase.
In plastic wash-basin, adorn a small amount of artificial feed, cover one plastic wire on the plastic wash-basin, there is the hole strawboard that has of pieces of an egg to be placed on the plastic wire product, onesize plastic wash-basin of the no end of upper cover, top plastic basin covers to keep humidity and to prevent that the fly worm from entering with wet gauze.Then, it is put into temperature 24--30 ℃, its temperature is bathed, the larva of hatching is fallen on the artificial feed automatically, the artificial feed that to collect newly hatched larvae every day are placed into the larva raising chamber and cultivate, and like this, can obtain the neat larva that grows.
The ovum of the ovum phase of Dallas's strain and Wuhan strain and Guangzhou strain significant difference between the phase (F=11.68, df=2, P=0.0085), short 2--3 days of the ovum phase of Dallas's strain, and Wuhan strain and Guangzhou between the strain ovum phase difference not remarkable, see Table 1.
Table 1 speck flat angle stratiomyiid Wuhan strain and Guangzhou strain and Dallas's strain ovum phase are relatively
All data are all analyzed with SAS1998, digitized representation experiment pieces of an egg number in the bracket.Experiment number of repetition=32, larval phase
(composition is wheat bran, alfalfa meal, corn flour with artificial feed, after mixing according to 5: 3: 2 ratios, progressively add water preparation, make water content reach 70%--80%, have little water from webs, to flow out when promptly clutching and get final product with hand) the flat angle of the speck stratiomyiid of three kinds of different strains of raising.The larva of hatching forwards to respectively in the washbowl that fills a small amount of artificial feed, getting 300 larvas after three days from every basin respectively is put in 3 washbowls that fill artificial feed, when the larva about 50% became propupa, larva on average heavily was that final larva is heavy at this moment, and average life cycle is larval phase.The artificial feed of collecting newly hatched larvae are put into larva culturing room, 24--30 ℃ of indoor maintenance temperature, humidity 75--80% according to worm size in age and density, suitably replenished fresh feed every 1--3 days.In the larva raising process, larval density is too big, and competition nutrition fierceness causes malnutrition between the larva, and larval growth is slow, irregular, and pupa development is bad, and eclosion rate is low; Larval density too hour, the intact easy moldy metamorphism of food utilization, larval growth is bad, causes infecting dead.Larval density is controlled at 1000--1500 head/kilogram feed generally speaking.
Larval phase significant difference between three strains (F=23.71, df=2, P=0.0014), the larval phase of Wuhan strain, is the shortest, and Dallas's strain larval phase is than the long 5-6 of Wuhan strain days, than the long 2-3 of Guangzhou strain days, the larval phase of Dallas's strain, is the longest, and Guangzhou strain larval phase is placed in the middle.The larval phase of three female worms of strain, is identical, all than long 1-2 of larval phase days of male worm.
Body weight significant difference (the F=134.23 of mature larva between three strains, df=2, P=0.001), Wuhan strain body weight is the highest, higher by about 20% than Guangzhou strain, high nearlyer 70% than Dallas strain, Wuhan strain mature larva is obviously all big than all the other two strains, this sees Table 2 to having clear superiority in the application such as feces of livestock and poultry conversion, production high-quality insect feedstuff.
Table 2 speck flat angle stratiomyiid Wuhan strain and Guangzhou strain and the larval phase comparison of Dallas's strain
And fixed, work is not remarkable mutually because sex * kind is to any variable according to the male and female sum for significant difference.All data are all analyzed with SAS1998, digitized representation experiment borer population in the bracket, experiment number of repetition=3
3, pupa time
(composition is wheat bran, alfalfa meal, corn flour with artificial feed, after mixing according to 5: 3: 2 ratios, progressively add the water preparation, make water content reach 70---80%, having little water to flow out from webs when promptly clutching with hand gets final product), raise the three kinds of flat angle of different strain specks stratiomyiids.The larva of hatching forwards to respectively in the washbowl that fills a small amount of artificial feed, gets 300 larvas after three days respectively and be put in 3 washbowls that fill artificial feed from every basin.Be prepupal period the average time when all the larva overstrike is pupated.Be pupa time the average time that 50% left and right sides pupa is sprouted wings.The male and female of mature larva, propupa, pupa are to distinguish according to the sex of its emergenceization back adult.
When the flat angle of speck stratiomyiid larva polypide is black by little xanthochromia, promptly begin to pupate, when the larva when 10% begins blackening, stop to add fresh feed.According to the characteristic of mature larva, will raise the plastic wash-basin of mature larva, to put into and receive the pupa plastic casing, pre-meconium climbs out of from basin, pupates voluntarily in receiving the pupa box.Because pupa contains lot of energy and earns a bare living and finish emergence, so the period of the pupa flat angle stratiomyiid biomass maximum in life cycle that is whole speck.The optimum environmental condition is 27 ℃ of temperature, relative moisture 70--80% during emergence.
(P=0.0085), the pupa time of Dallas's strain, difference was not remarkable between Guangzhou strain and the Wuhan strain than short 2-3 days of all the other two strains for F=11.70, df=2 for significant difference in pupa time between three strains.There is not notable difference the pupa time of female worm and male worm.(P=0.0004), the propupa of Wuhan strain is heavily more about 43% than Dallas strain for F=36.84, df=2, and the propupa weight ratio Wuhan strain of Guangzhou strain is low, than Dallas's strain height for significant difference between three strains of the weight of propupa.The propupa of female worm is bigger than the weight of male worm, sees Table 3.
Table 3 speck flat angle stratiomyiid Wuhan strain and Guangzhou strain and Dallas's strain compare pupa time
And fixed, work is not remarkable mutually because sex * kind is to any variable according to the male and female sum for significant difference.All data are all analyzed with SAS1998, digitized representation experiment borer population in the bracket, experiment number of repetition=3
4, the adult stage
Pupa is put into 30 * 30 * 30 centimetres worm cage respectively, from 50% left and right sides pupa turn into adult to average time of 50% left and right sides adult death be the adult stage.The male and female quantity of pupa and adult is to determine according to the sex of the back adult that sprouts wings.In order to adapt to the life habit of stratiomyiid, in the worm cage, hang a little green plantss, be convenient to their mating, every female worm 200-800 grain of can laying eggs.In the worm cage, put a basin feed, the basin edge is stained with the horsehit carton in hole, so that ovum is produced in the hole.
Significantly (P=0.0005), all the other two strains of the adult weight ratio of Dallas's strain are low for F=34.74, df=2, and difference is little between Guangzhou, two strains in Wuhan for adult weight interspecific difference.Female worm all weighs than male worm in three strains.Influence between the life-span of adult also significantly is subjected to planting, the Wuhan strain life-span is more longer than all the other two strain life-spans, and Dallas's strain life-span is the shortest, and interspecific difference is all very remarkable between three strains, sees Table 4.
Table 4 speck flat angle stratiomyiid Wuhan strain and Guangzhou strain and Dallas's strain adult stage are relatively
And fixed, work is not remarkable mutually because sex * kind is to any variable according to the male and female sum for significant difference.All data are all analyzed with SAS1998, digitized representation experiment borer population in the bracket, experiment number of repetition=3
Embodiment 3: stratiomyiid Wuhan, the flat angle of speck strain, Guangzhou strain and Dallas's strain are handled the comparison of pig manure, chicken manure and cow dung conversion capability respectively
1, three strains of the flat angle of speck stratiomyiid transform the comparison of pig manure, chicken manure and cow dung larva weight
(1) the Wuhan strain transforms the comparison of pig manure, chicken manure and cow dung larva weight
When three kinds of different ight soil transform, the larva output interspecific difference that is obtained from three kinds of ight soil shows (F=734.65, df=2, P＜0.0001), the highest from the larva dry weight of chicken manure kind gained, the larva that is obtained in cow dung is minimum, the larva dry weight of gained is placed in the middle in the pig manure, the larva dry weight of gained is 2.5 times of cow dung in the chicken manure, Duos about 20% than the larva dry weight that obtains in the pig manure, sees Table 5.
(2) the Guangzhou strain transforms the comparison of pig manure, chicken manure and cow dung larva weight
Utilize the Guangzhou strain to pig manure, chicken manure, when three kinds of different ight soil of cow dung transform, the larva output interspecific difference that is obtained from three kinds of ight soil shows (F=19 81.34.df=2, P＜0.0001), the larva dry weight of gained is the highest from chicken manure, the larva that is obtained in cow dung is minimum, and the larva dry weight of gained is placed in the middle in the pig manure, and the larva dry weight of gained is 2.3 times of cow dung in the chicken manure, Duo about 25% than the larva dry weight that obtains in the pig manure, see Table 5.
(3) Dallas's strain transforms the comparison of pig manure, chicken manure and cow dung larva weight
Utilize Dallas's strain to pig manure, chicken manure, when three kinds of different ight soil of cow dung transform, the larva output interspecific difference that is obtained from three kinds of ight soil is (F=1776.89 significantly, df=2, P＜0.0001), the highest from the larva dry weight of chicken manure kind gained, the larva that is obtained in cow dung is minimum, the larva dry weight of gained is placed in the middle in the pig manure, more than the larva dry weight of gained is three times of cow dung in the chicken manure, Duo about 30%, see Table 5 than the larva dry weight that obtains in the pig manure.
(4) transform the comparison of pig manure, chicken manure and cow dung larva weight between three strains of the flat angle of speck stratiomyiid
When utilizing Wuhan strain, Guangzhou strain and Dallas's strain to transform pig manure, the significant difference between the larva output that obtains (F=68.44, df=2, P＜0.0001), the dry weight of Wuhan strain larva is the highest, and the Guangzhou strain is placed in the middle, and Dallas's strain is minimum.When utilizing Wuhan strain, Guangzhou strain and Dallas's strain to transform chicken manure, the significant difference between the larva dry weight that obtains (F=258.31, df=2, P＜0.0001), the dry weight of Wuhan strain larva is the highest, and the Guangzhou strain is placed in the middle, and Dallas's strain is minimum.When utilizing Wuhan strain, Guangzhou strain and Dallas's strain to handle cow dung, Dallas's strain transforms the dry weight of larva that cow dung obtains, significantly is lower than Wuhan strain and Guangzhou strain (F=34.69, df=2, and difference is not remarkable between Wuhan strain and the Guangzhou strain P＜0.0001).
Table 5 speck flat angle stratiomyiid Wuhan strain and Guangzhou strain and Dallas's strain transform pig manure, chicken manure
Comparison with cow dung larva weight
All data are all analyzed with SAS1998, experiment number of repetition=3
2, three strains of the flat angle of speck stratiomyiid transform pig manure, chicken manure and cow dung dry matter reduction
The Wuhan product tie up to when transforming pig manure, chicken manure, these three kinds of ight soil of cow dung, ight soil dry matter slip is respectively 53.4%, 61.7%, 57.8%, the Guangzhou product tie up to when transforming pig manure, chicken manure, these three kinds of ight soil of cow dung, and ight soil dry matter slip is respectively 28.8%, 31.8%, 34.6%.Dallas's product tie up to when transforming pig manure, chicken manure, these three kinds of ight soil of cow dung, and ight soil dry matter slip is respectively 49.7%, 56.8%, 53.6%.Same product tie up to when transforming three kinds of different ight soil, and best to the changing effect of chicken manure, the dry matter slip of chicken manure is the highest, and the changing effect of cow dung is the poorest, and the dry matter slip is minimum, sees Table 6.
Ight soil dry matter and nitrogen content reduce after table 6 speck flat angle stratiomyiid Wuhan strain and Guangzhou strain and the conversion of Dallas's strain pig manure, chicken manure and the cow dung
When three different product tie up to conversion with a kind of ight soil, the changing effect of Wuhan strain is best, the ight soil dry matter reduction of making conversion of the Wuhan strain is the highest, the transformation efficiency of Dallas's strain is the poorest, the ight soil dry matter reduction of making conversion of Dallas's strain is minimum, and the transformation efficiency of Guangzhou strain is placed in the middle, and the ight soil dry matter reduction of making conversion of the Guangzhou strain is placed in the middle.And in the time of can finding with Wuhan strain processing chicken manure, changing effect is best, and the effect that transforms cow dung with Dallas's strain is the poorest.
3, three strains of the flat angle of speck stratiomyiid transform pig manure, chicken manure and cow dung nitrogen content reduction
The Wuhan product tie up to when transforming pig manure, chicken manure, these three kinds of ight soil of cow dung, ight soil nitrogen content slip is respectively 47.9%, 51.8%, 50.6%, the Guangzhou product tie up to when transforming pig manure, chicken manure, these three kinds of ight soil of cow dung, ight soil nitrogen content slip is respectively 49.9%, 56.0%, 53.2%, Dallas's product tie up to when transforming pig manure, chicken manure, these three kinds of ight soil of cow dung, and ight soil nitrogen content slip is respectively 22.1%, 24.8%, 25.8%.Identical strain stratiomyiid is when transforming three kinds of different ight soil, and best to the changing effect of chicken manure, the nitrogen content slip of chicken manure is the highest, and the changing effect of cow dung is the poorest, and the nitrogen content slip is minimum.
When three different product tied up to conversion with a kind of ight soil, the changing effect of Wuhan strain was best, and the nitrogen content reduction is the highest, and the transformation efficiency of Dallas's strain is the poorest, and the nitrogen content reduction is minimum, and the transformation efficiency of Guangzhou strain is placed in the middle, and the nitrogen content reduction is placed in the middle.And in the time of can finding with Wuhan strain conversion chicken manure, changing effect is best, and the effect of handling cow dung with Dallas's strain is the poorest, sees Table 6.
Embodiment 4: stratiomyiid Wuhan, the flat angle of speck strain, Guangzhou strain and Dallas's strain transform after pig manure, chicken manure and the cow dung larva polypide protein content and amino acid respectively and form
1, the comparison of larvae protein content after Wuhan strain conversion pig manure, chicken manure and the cow dung
When utilizing the Wuhan strain to transform pig manure, chicken manure, cow dung, significant difference (F=5.99 between the larvae protein content that is obtained, df=2, P=0.0302), the highest from the protein content of larva that chicken manure obtains, placed in the middle from the protein content of larva that pig manure obtains, minimum from the protein content of larva that cow dung obtains, see Table 7.
2, the comparison of larvae protein content after Guangzhou strain conversion pig manure, chicken manure and the cow dung
When utilizing the Guangzhou strain to transform pig manure, chicken manure, cow dung, significant difference (F=4.63 between the protein content of the larva that obtains, df=2, P=0.0608), the highest from the protein content of larva that pig manure obtains, placed in the middle from the protein content of larva that chicken manure obtains, minimum from the protein content of larva that cow dung obtains, see Table 7.
3, the comparison of larvae protein content after Dallas's strain conversion pig manure, chicken manure and cow dung
When utilizing Dallas's strain to transform pig manure, chicken manure, cow dung, the larvae protein content interspecific difference that is obtained is (F=22.46 significantly, df=2, P=0.0016), the larva protein content that grows in the chicken manure is the highest, the larva protein content that grows in the pig manure is placed in the middle, and the larva protein content that grows in the cow dung is minimum, sees Table 7.
4, the comparison between the larvae protein content after three strains conversions of the flat angle of speck stratiomyiid pig manure, chicken manure and cow dung
When utilizing Wuhan strain, Guangzhou strain and Dallas's strain to transform pig manure, significantly (P=0.1176), and protein content difference is very not little for F=3.12, df=2 for the difference between three strain larvae protein content; When transforming chicken manure, the difference between three strain larvae protein content also not significantly (F=0.60, df=2, P=0.5797); When transforming cow dung, between three strain larvae protein content difference not significantly (F=3.08, df=2, P=0.1199).Show that the different strains of the flat angle of speck stratiomyiid handle different ight soil, gained larvae protein content difference is not remarkable.
Larva polypide total protein and protein content that the different strains of the flat angle of table 7 speck stratiomyiid transform after pig manure, chicken manure and the cow dung compare
All data are all analyzed with SAS1998,
bThe n=sample size, number of repetition=3
In sum: when Wuhan strain, Guangzhou strain and Dallas's strain transformed with a kind of ight soil, the conversion capability of Wuhan strain is apparently higher than Dallas's strain, and was also higher than Guangzhou strain; The weight of the larva that obtains when different strains transform pig manure and chicken manure, the Wuhan strain is the highest, and the Guangzhou strain is placed in the middle, and Dallas's strain is minimum; The larva weight that different strains are obtained when transforming cow dung, Wuhan strain and Guangzhou strain are higher, and Dallas's strain is minimum.This is because the Wuhan strain more adapts to natural conditions such as local climate.When different strains were handled with a kind of ight soil, the protein content of gained larva did not have notable difference, and when handling different ight soil with a kind of strain, the protein content difference of gained larva is also not obvious.
When same strain was handled different ight soil, the changing effect of chicken manure was best, and larva dry matter weight and protein content are the highest, and the changing effect of cow dung is the poorest, and dry matter weight and protein content are minimum.When finding also that simultaneously three product tie up to processing with a kind of ight soil, the changing effect of Wuhan strain is best, and dry matter weight and the protein content of larva are the highest.
5, amino acid is formed
The larvae protein amino acid that stratiomyiid Wuhan, the flat angle of speck strain, Guangzhou strain and Dallas's strain transform respectively after pig manure, chicken manure and the cow dung is formed, as can be seen from Table 8, it is little that the amino acid of larva is formed difference between three strains, and each aminoacid ingredient shared percentage difference in total amino acid content separately is little in each strain.The total amino acid content of three different strains is variant, wherein the total amino acid content of Guangzhou strain is 39.52%, the total amino acid content of Wuhan strain is 37.81%, and the total amino acid content of Dallas's strain is 35.66%, this shows that the total amino acid content of Dallas's strain is minimum.The essential amino acids content of three strains is also variant, and the essential amino acids content of Dallas's strain is minimum as can be seen from Table 8, and the Wuhan strain is placed in the middle, and the Guangzhou strain is the highest.
The amino acid of the different strain larvae proteins of the flat angle of table 8 speck stratiomyiid is formed
*, primary amino acid
Embodiment 5: larva body fat content and fatty acid that stratiomyiid Wuhan, the flat angle of speck strain, Guangzhou strain and Dallas's strain transform respectively after pig manure, chicken manure and the cow dung are formed
1, the fat content of larva after the different strains conversion of the flat angle of speck stratiomyiid pig manure, chicken manure, the cow dung
Stratiomyiid Wuhan, the flat angle of speck strain, Guangzhou strain and Dallas's strain transform pig manure, chicken manure and cow dung larva fat content respectively.As seen from Table 9, when three different strains transformed pig manure, chicken manure and cow dung, total fat mass of Wuhan strain and Guangzhou strain was apparently higher than Dallas's strain; When transforming pig manure and chicken manure, strain total fat mass in Wuhan is higher than the Guangzhou strain; Wuhan strain and the total fat mass indifference of Guangzhou strain when transforming cow dung.Three strain conversion pig manures, chicken manure and cow dung larva fat content difference are little.When transforming pig manure and cow dung, Wuhan strain fat content is the highest; When transforming chicken manure, Guangzhou strain fat content is the highest.
Larva polypide fat content and total fat are relatively after the different strains conversion of the flat angle of table 9 speck stratiomyiid pig manure, chicken manure and the cow dung
All data are all analyzed with SAS1998,
bThe n=sample size, number of repetition=3
In sum: after handling with a kind of ight soil, the amount of total fat of the flat angle of the speck of Wuhan strain stratiomyiid larva is higher than Guangzhou strain and Dallas's strain.The disposal ability that the Wuhan strain is described is apparently higher than Dallas's strain, and is also higher than Guangzhou strain, and this may be owing to the natural conditions such as weather of Wuhan strain to Wuhan Area more adapt to.
2, the fatty acid of larva fat is formed behind stratiomyiid Wuhan, the flat angle of the speck strain conversion pig manure
The fatty acid composition and the content of larva fat see Table 10 behind stratiomyiid Wuhan, the flat angle of the speck strain conversion pig manure.
The fatty acid of the different strain larva of the flat angle of table 10 speck stratiomyiid fat is formed
Result in the table shows, be not both at fatty acid composition and content and other insect: the maximum characteristics of stratiomyiid Wuhan strain larva fat are to contain polytype unsaturated fatty acid, and they are C16:1, C18:2, C18:3, C18:4, C18:1, C20:1, C20:2, C22:1.And saturated fatty acid is based on palmitic acid (C16:0), and the content of stearic acid (C18:0) is lower, and the monounsaturated fatty acids overwhelming majority forms with oleic acid (C18:1), and that content is maximum is maleic acid (C12:0).These are different relevant with caste, and the special composition of fatty acid of stratiomyiid larva fat has important application aspect health products.And the fatty acid composition of stratiomyiid Wuhan strain larva fat and content and Dallas's strain difference are little.
Claims (8)
1. the domesticating and cultivating method of stratiomyiid Wuhan, the flat angle of speck strain, its step is as follows:
(1) the outdoor collection that lures of wild stratiomyiid
Near the accumulation of suburbs, Wuhan has the place of feces of livestock and poultry, fill chicken manure with plastic basin, use the branch proping, the plastic basin of filling chicken manure is placed on the support, avoid the interference of carnivorous insects such as ant, lure the flat angle of speck stratiomyiid adult to lay eggs, the hatching back is collected larva and is taken back in the laboratory;
Or near the accumulation of suburbs, Wuhan has the place of feces of livestock and poultry, fill chicken manure with plastic basin, plastic wire of cover on plastic basin lures the flat angle of speck stratiomyiid adult to get food then, the adult that captures taken back the laboratory and place the worm cage of 40cm * 40cm * 120 cm, make its mate and oviposit;
(2) the indoor domestication of wild stratiomyiid
With the larva of field acquisition and/or the larva that lures integrated worm to lay eggs to be hatched with artificial feed at room temperature 24-30 ℃, carry out artificial feeding under the humidity 75--80%, selecting healthy and strong larva raising from the larva that survives puts in the worm cage of illumination after pupating, turn into adult, regularly, guarantee that humidity is at 75--80% toward worm cage water spray; On overcast and rainy daytime, stimulate the adult mating with the iodine-tungsten lamp irradiation, adult post-coitum self-sow is to laying eggs, under natural lighting or iodine-tungsten lamp irradiation, collect worm's ovum, the raising that uses the same method of the larva of ovum hatching, domestication was raised more than 10 generations so repeatedly, obtain the larva bodily form, size unanimity, the adult bodily form, stable performance of the same size colony, identify, by morphological feature to female insect and male insect, and the evaluation of larva form, be defined as the flat angle of stratiomyiid subfamily Hermetia speck, the flat angle of Stratiomyidae stratiomyiid Wuhan strain WH.
2. domesticating and cultivating method according to claim 1 is characterized in that: described feces of livestock and poultry is chicken manure or pig manure or cow dung.
3. domesticating and cultivating method according to claim 1 is characterized in that: described chicken manure is to have common house-fly grub to get the chicken manure of food.
4. according to arbitrary described domesticating and cultivating method among the claim 1-3, it is characterized in that: described artificial feed are that to add water after wheat bran, alfalfa meal, corn flour mix with the weight ratio of 5:3:2 formulated, and water content reaches 70-80%.
5. the application of the flat angle of the speck stratiomyiid Wuhan strain WH that domesticating and cultivating method according to claim 1 is cultured in converting animal-poultry faeces accumulates with the reduction feces of livestock and poultry.
6. application according to claim 5 is characterized in that: described feces of livestock and poultry is chicken manure or pig manure or cow dung.
7. according to claim 5 or 6 described application, it is characterized in that concrete application method is as follows: the flat angle of speck stratiomyiid Wuhan strain WH is inserted in the feces of livestock and poultry according to the ratio larva of 1000/kg, raise 40% larva and begin to pupate.
8. application according to claim 7 is characterized in that: the prepupa that obtains is collected and processes handle the back as nutrient and healthcare products or animal protein feed.
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