CN107410213A - A kind of method for propagating Monochamus alternatus artificially - Google Patents
A kind of method for propagating Monochamus alternatus artificially Download PDFInfo
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- CN107410213A CN107410213A CN201710324580.3A CN201710324580A CN107410213A CN 107410213 A CN107410213 A CN 107410213A CN 201710324580 A CN201710324580 A CN 201710324580A CN 107410213 A CN107410213 A CN 107410213A
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a kind of method for propagating Monochamus alternatus artificially, for captive breeding Monochamus alternatus under laboratory condition.Methods described includes：（1）The artificial feeding of the sub- ripe individual of Monochamus alternatus；（2）The outfit in the artificial delivery room of Monochamus alternatus；（3）The artificial pairing raising and induction breeding of Monochamus alternatus sexal maturity individual；（4）The preparation of each instar larvae man-made feeds of Monochamus alternatus；（5）The separation of the nascent larva of Monochamus alternatus obtains；（6）Alternatus Larva is transferred to the follow-up cultivation of man-made feeds；（7）Monochamus alternatus pupa time and management of sprouting wings.The method for the artificial farming Monochamus alternatus that the present invention uses, a set of system for being applied to manually cultivate the Monochamus alternatus of each growth phase is constructed in laboratory conditions, while pine consumption is reduced, significantly improve the survival rate of each growth phase of Monochamus alternatus, exclude influence of the season to Monochamus alternatus growth and breeding, shorten the growth cycle of Monochamus alternatus, form stable experiment worm sources.
Description
Technical field
The invention belongs to experimental animal to cultivate field, and in particular to a kind of method for propagating Monochamus alternatus artificially.
Background technology
Monochamus alternatus is a kind of primary pest of pine tree, widely distributed in China or even world wide.Larva moth food tree
Dry, adult gnaws bark, causes tree vigo(u)r weak, and severe patient can cause whole strain pine tree is withered to fracture.Monochamus alternatus still results in simultaneously
Effective communication media of the withered sick direct arch-criminal pine wood nematode of pine tree, pine wood nematode is by colonizing in the tracheal strips of Monochamus alternatus
Migrated with Monochamus alternatus between pine forest, the whole strain of pine tree by severe infections is withered in shape is burnt.Annual Monochamus alternatus and pine
Nematodiasis causes huge direct economic loss, therefore significantly more efficient preventing and treating Monochamus alternatus and pine nematode to Foresty industry
It is extremely urgent.
The method of the preventing and treating Monochamus alternatus used at present mainly has mass trapping, epidemic disease dowel to ruin, pesticide application and biological control
Deng wherein biological control includes Feeding behavior preventing and treating and fungus preventing and treating, and muscardine, bacillus thuringiensis etc. are to Monochamus alternatus again
There is certain toxic action, the adult and larva to Monochamus alternatus are all effective, and the harmful effect to environment prevents and treats compared with other
Means are lower.During efficient means of prevention is found, the Monochamus alternatus for using substantial amounts of each worm state is undoubtedly needed to be used for real
Test, traditional field acquisition Monochamus alternatus imago, the method for alternatus Larva is separated from epidemic disease wood to waste time and energy, and Yin Jijieyin
The more difficult Monochamus alternatus that each worm state is obtained in annual any time of element.If artificial farming pine ink in laboratory conditions can be realized
Longicorn, huge help will be brought to the research work that Monochamus alternatus is prevented and treated.
For the biological characteristics of Monochamus alternatus, using a kind of method for propagating Monochamus alternatus artificially, in laboratory condition
The lower raising for carrying out each worm state of Monochamus alternatus and breeding passage, reduces each side consumption, and solving seasonal factor influences asking for experiment
Topic, stable laboratory Monochamus alternatus population is established, provided for the work of the study on prevention of Monochamus alternatus and pine nematode strong
Ensure.
The content of the invention
It is an object of the invention to provide a kind of method for propagating Monochamus alternatus artificially, excludes seasonal factor to Monochamus alternatus
The influence of related experiment, each side consumption of field acquisition Monochamus alternatus is reduced, builds stable Monochamus alternatus Artificial Population.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of method for propagating Monochamus alternatus artificially, methods described include as follows：
（1）The artificial feeding of the sub- ripe individual of Monochamus alternatus；
（2）The outfit in the artificial delivery room of Monochamus alternatus；
（3）The artificial pairing raising and induction breeding of Monochamus alternatus sexal maturity individual；
（4）The preparation of each instar larvae man-made feeds of Monochamus alternatus；
（5）The separation of the nascent larva of Monochamus alternatus obtains；
（6）Alternatus Larva is transferred to the follow-up cultivation of man-made feeds；
（7）Monochamus alternatus pupa time and management of sprouting wings.
Comprise the following steps that：
1st, Monochamus alternatus trap trapping wild Monochamus alternatus live body is hung in Monochamus alternatus generation area pine forest；Or from epidemic disease
Aging alternatus Larva is separated in wood, 12 milliliters of EP test tubes is taken, inserts natural wood pulp paper handkerchief, add water to soak, will be aging
Alternatus Larva is positioned over temperature as 25 ~ 28 DEG C as in this pipe, and by the test tube equipped with Monochamus alternatus mature larva, humidity
Dark culturing in 65 ~ 80% insulating box, mature larva is promoted to pupate and finally sprout wings.
2nd, prepare length and width be respectively delivery room of 50 ~ 60CM, a height of 80 ~ 120CM the grenadine cage as longicorn, in cage in
Put a 500ML triangular flasks, injected clear water, using water planting in fresh pine tree branch insertion triangular flask as nutrition branch in centre；In cage
An a diameter of 5 ~ 10CM is respectively placed in interior corner, length is 15 ~ 20CM and the fresh healthy masson pine belt leather wood of sealing two ends
Duan Zuowei spawning wood.By above-mentioned Monochamus alternatus delivery room be placed in temperature be 25 ~ 28 DEG C, humidity be 65 ~ 80%, photoperiod be illumination：It is black
Secretly=12:In 12h greenhouse.
3rd, by Monochamus alternatus imago according to two female one it is male be one group, be put into ready delivery room, allow its panmixia, production
Ovum.Each delivery room is put into five to six group Monochamus alternatus imagos.One time of nutrition branch and spawning wood are changed every three days, and are being laid eggs
Time mark is carried out on wood.
4th, after the Monochamus alternatus mature larva isolated in epidemic disease wood is sprouted wings, a small size plastics goldfish jar is taken as raising
Box, paper handkerchief adds water to soak as moisturizing material on the pad of bottom；One section of 10CM of interception or so nutrition branch is put into conduct in box
The feed of new emergence subadult adult.The subadult adult of emergence is put into above-mentioned raising box, per one, box, is placed in and delivery room phase
With environment under raise seven to ten days and does the same terms with the direct Monochamus alternatus imago traped to delivery room is transferred to after sexal maturity
Mate raising, breeding.
5th, the spawning wood changed in delivery room is placed in the greenhouse of the same terms after continuing culture 12 ~ 15 days, with wood
Icking tool carefully peels off bark and xylem, takes out the nascent alternatus Larva stayed and be born in bast.
6th, the bark for the spawning wood for isolating alternatus Larva and xylem are crushed to below 40 mesh respectively, made
It is standby into xylem powder, bark fines, it is standby；Assignment configuration is into the Monochamus alternatus low instar larvae feed in 1 ~ 2 age and after 3 ages
Monochamus alternatus advanced age larvae feeds；
The Monochamus alternatus low instar larvae feed in 1 ~ 2 described age includes following raw material：45 ~ 55 parts of xylem powder, tree
90 ~ 110 parts of skin powder, 60 ~ 70 parts of one or more in corn flour, soy meal, wheat bran, edible fungus bran, benzoic acid, benzene first
2 ~ 3 parts of one or more in sour sodium, sorbic acid, potassium sorbate, dry ferment, dusty yeast, one kind or several of yeast extract kind
20 ~ 30 parts of kind, 25 ~ 35 parts of agar strip, 8 ~ 15 parts of shrimp shell meal, the Fen , Unit of sucrose 35 ~ 45 steam 280 ~ 320 parts of water；3 described ages with
Monochamus alternatus advanced age larvae feeds afterwards include following raw material：140 ~ 160 parts of xylem powder, corn flour, soy meal,
60 ~ 70 parts of one or more in wheat bran, edible fungus bran, one kind in benzoic acid, sodium benzoate, sorbic acid, potassium sorbate
Or several 2 ~ 3 parts, dry ferment, dusty yeast, 20 ~ 30 parts of the one or more of yeast extract kind, 25 ~ 35 parts of agar strip, shrimp shell
8 ~ 15 parts of powder, the Fen , Unit of sucrose 35 ~ 45 steam 280 ~ 320 parts of water.
7th, agar strip dissolving is heated into being placed in aluminum pot in addition to xylem powder and bast powder by above-mentioned, respectively
Composition is mixed as uniform paste,；45 ~ 55 parts of xylem powder, 90 ~ 110 parts of bark fines is taken to be mixed with mentioned component paste, it is cold
But insert in 12MLEP test tubes and be filled to away from the capacity of the mouth of pipe 1/3rd afterwards, capping, Monochamus alternatus low instar larvae is made and manually raises
Material.Take 140 ~ 160 parts of xylem powder to be mixed with above-mentioned composition paste, insert in 50ML centrifuge tubes and be filled to away from the mouth of pipe after cooling
At 1/3rd capacity, capping, the middle advanced age larva artificial feed that Monochamus alternatus was transferred to after 3 ages is made.Above two is artificial
Feed is put into autoclave, 121 degrees Celsius sterilizing 30 minutes after take out cooling it is standby.
8th, the tweezers sterilized in the center of the low instar larvae man-made feeds dispensed prick an aperture, will be isolated
Nascent alternatus Larva head be lowered into aperture, cover centrifugation lid, be placed in artificial with delivery room environmental condition identical
Cultivated in climate box.The feeding situation of routine observation alternatus Larva, will carefully or so 3 initial stages in age be grown to after two weeks
Alternatus Larva takes out, and completes the raising in low age stage, afterwards for above-mentioned same method by the alternatus Larva of taking-up
It is transferred in advanced age larva artificial feed and carries out follow-up cultivation, the feeding situation and growing state of routine observation Monochamus alternatus, directly
Into the man-made feeds of Monochamus alternatus it is smooth go out pupa room pupate, finally sprout wings a new generation adult,
9th, the adult newly to sprout wings is put into single cage in goldfish jar raising box to raise 7 ~ 10 days, is transferred to delivery room mating, spawning, completes
One wheel raising.It is by the operation in batches of Monochamus alternatus imago, the growth phase of Monochamus alternatus is interleaved, reach while possess each
The Monochamus alternatus of worm state and growth phase.Constantly circulation above procedure, realize the laboratory artificial cultivating of Monochamus alternatus.
The advantage of the invention is that：
1st, by using a kind of method of artificial feeding Monochamus alternatus of the present invention, with existing patent（CN 10228853
B；CN 101983580 A;The A of CN 102715377) and the article reported《Monochamus alternatus indoors artificial is raised and its biology
Learn characteristic observation》Described in the method for artificial feeding Monochamus alternatus compare, improve the utilization rate of pine, use mushroom bran, wheat
The cheap raw material such as bran, has saved feeding cost and manpower and materials, with the addition of in alternatus Larva feed appropriate
The shrimp shell composition of chitin is supplemented, also more science, the feed of adult also more meet the feeding of Monochamus alternatus to other constituents
Habit, it is more conducive to the growth of Monochamus alternatus.The passage cultivation of Monochamus alternatus is realized in laboratory conditions, it is established that stable
Longicorn population is tested, the growth cycle of Monochamus alternatus is shortened, is not influenceed by seasonal factor, accomplish that each worm can be provided at any time
State, the Monochamus alternatus of each age are used for experimental study.Greatly reduce using traditional field acquisition and separation Monochamus alternatus and produce
The consumption of raw human and material resources and financial resources, accelerates Monochamus alternatus and preventing and controlling pine wilt disease studies the process organized altogether, is forest products
Industry makes tremendous contribution.
2nd, by using the inventive method, the purifying of Monochamus alternatus strain can be carried out.With the Monochamus alternatus phase of field acquisition
Than, the Monochamus alternatus of this method raising can reduce influence of the self-contained pathogenic microorganism of longicorn to experiment as far as possible,
It is a kind of preferable experiment material.
Brief description of the drawings
Fig. 1 single cages are raised（Left figure）And delivery room（Right figure）Schematic diagram.
Fig. 2 larva artificial feeds（Left figure）And high instar larvae in being grown in feed（Right figure）Schematic diagram.
Monochamus alternatus in Fig. 3 mating（Left figure）And the female Monochamus alternatus laid eggs（Right figure）.
Embodiment
Embodiment 1
9 to during October arrive Monochamus alternatus generating region collection by Monochamus alternatus eat into food branch, trunk, by Monochamus alternatus from
Middle separation, it is placed in the EP test tubes equipped with moist tissue, it is 27 DEG C to set environment temperature, and humidity 70%, camera bellows culture is until plumage
Turn to adult.The adult just to sprout wings is placed in the raising box for being lined with moist tissue, raised with fresh branch section, is raised to after ten days
It is put into according to a two female heros for one group in ready delivery room, each delivery room is put into five groups of Monochamus alternatus imagos.Every three days more
The nutrition branch that changes in a delivery room and spawning wood, spawning wood is carried out into time mark, be placed in delivery room under the same conditions after
Continuous culture 15 days, the bark for wood of laying eggs is peelled off with icking tool, takes out the raw Monochamus alternatus low instar larvae in bast of moth, transfer into
Cultivate two weeks, then be transferred in ready Monochamus alternatus in advanced age larvae feeds in ready Monochamus alternatus low instar larvae feed
Culture is to sprouting wings, into next raising circulation.The repoductive time of Monochamus alternatus imago is staggered so that offspring's Monochamus alternatus
Growth phase staggers therewith, realizes and possesses each worm state, the Monochamus alternatus of each growth phase simultaneously.The Song Motian of the present embodiment culture
Ox eclosion rate is up to 83.5%.
The Monochamus alternatus low instar larvae feed in 1 ~ 2 age includes following raw material：50 parts of xylem powder, bark fines
100 parts, 65 parts of corn flour, 2 parts of benzoic acid, 25 parts of dry ferment, 30 parts of agar strip, 10 parts of shrimp shell meal, the Fen , Unit of sucrose 40 steaming water
300 parts；
Monochamus alternatus advanced age larvae feeds after 3 ages include following raw material：150 parts of xylem powder, soy meal 65
Part, 3 parts of sodium benzoate, 25 parts of dusty yeast, 30 parts of agar strip, 10 parts of shrimp shell meal, the Fen , Unit of sucrose 40 300 parts of water of steaming.
Embodiment 2
1st, Monochamus alternatus trap trapping wild Monochamus alternatus live body is hung in Monochamus alternatus generation area pine forest.
2nd, it is respectively delivery room of 60CM, a height of 120CM the grenadine cage as longicorn to prepare length and width, and center puts one in cage
500ML triangular flasks, injected clear water, using water planting in fresh pine tree branch insertion triangular flask as nutrition branch；The corner in cage
Respectively place an a diameter of 10CM, length is 20CM and the fresh healthy masson pine belt leather juggle of sealing two ends is as spawning
Wood.By above-mentioned Monochamus alternatus delivery room be placed in temperature be 25 DEG C, humidity 65%, photoperiod be illumination：Dark=12:12h greenhouse
It is interior.
3rd, by Monochamus alternatus imago according to two female one it is male be one group, be put into ready delivery room, allow its panmixia, production
Ovum.Each delivery room is put into six groups of Monochamus alternatus imagos.One time of nutrition branch and spawning wood are changed every three days, and on spawning wood
Carry out time mark.
4th, after the Monochamus alternatus mature larva isolated in epidemic disease wood is sprouted wings, a small size plastics goldfish jar is taken as raising
Box, paper handkerchief adds water to soak as moisturizing material on the pad of bottom；One section of 10CM of interception nutrition branch, which is put into box, is used as new plumage
Change the feed of subadult adult.The subadult adult of emergence is put into above-mentioned raising box, per one, box, is placed in and delivery room identical
Raise under environment seven to ten days and mate to being transferred to delivery room after sexal maturity and do the same terms with the Monochamus alternatus imago directly traped
Raising, breeding.
5th, the spawning wood changed in delivery room is placed in the greenhouse of the same terms after continuing culture 15 days, uses woodcut knife
Carefully bark and xylem are peeled off, take out the nascent alternatus Larva stayed and be born in bast.
6th, the bark for the spawning wood for isolating alternatus Larva and xylem are crushed to below 40 mesh respectively, made
It is standby into xylem powder, bark fines, it is standby；Assignment configuration is into the Monochamus alternatus low instar larvae feed in 1 ~ 2 age and after 3 ages
Monochamus alternatus advanced age larvae feeds；
The Monochamus alternatus low instar larvae feed in 1 ~ 2 described age includes following raw material：45 parts of xylem powder, bark powder
90 parts of end, 60 parts of soy meal, 2 parts of sorbic acid, 20 parts of dusty yeast, 25 parts of agar strip, 8 parts of shrimp shell meal, the Fen , Unit of sucrose 35 steam water
280 parts；Monochamus alternatus advanced age larvae feeds after 3 described ages include following raw material：140 parts of xylem powder,
60 parts of wheat bran, 3 parts of potassium sorbate, 20 parts of dusty yeast, 25 parts of agar strip, 8 parts of shrimp shell meal, the Fen , Unit of sucrose 35 steam 280 parts of water.
7th, agar strip dissolving will be heated into being placed in aluminum pot outside xylem powder and bast powder, each composition mixes
For uniform paste, add appropriate monovalent sulfuric acid and adjust to pH=5；Take xylem powder, bark fines and mentioned component paste
Mixing, insert in 12MLEP test tubes and be filled to away from the capacity of the mouth of pipe 1/3rd after cooling, capping, Monochamus alternatus low instar larvae is made
Man-made feeds.Take xylem powder part to be mixed with above-mentioned composition paste, insert in 50ML centrifuge tubes and be filled to away from the mouth of pipe after cooling
At 1/3rd capacity, capping, the middle advanced age larva artificial feed that Monochamus alternatus was transferred to after 3 ages is made.Above two is artificial
Feed is put into autoclave, 121 degrees Celsius sterilizing 30 minutes after take out cooling it is standby.
8th, the tweezers sterilized in the center of the low instar larvae man-made feeds dispensed prick an aperture, will be isolated
Nascent alternatus Larva head be lowered into aperture, cover centrifugation lid, be placed in artificial with delivery room environmental condition identical
Cultivated in climate box.The feeding situation of routine observation alternatus Larva, will carefully or so 3 initial stages in age be grown to after two weeks
Alternatus Larva takes out, and completes the raising in low age stage, afterwards for above-mentioned same method by the alternatus Larva of taking-up
It is transferred in advanced age larva artificial feed and carries out follow-up cultivation, the feeding situation and growing state of routine observation Monochamus alternatus, directly
Into the man-made feeds of Monochamus alternatus it is smooth go out pupa room pupate, finally sprout wings a new generation adult,
9th, the adult newly to sprout wings is put into single cage in goldfish jar raising box to raise 10 days, is transferred to delivery room mating, spawning, completes one
Wheel raising.It is by the operation in batches of Monochamus alternatus imago, the growth phase of Monochamus alternatus is interleaved, reach while possess each worm
The Monochamus alternatus of state and growth phase.Constantly circulation above procedure, realize the laboratory artificial cultivating of Monochamus alternatus.Use this reality
The Monochamus alternatus eclosion rate of example culture is applied up to 86.0%.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, it should all belong to the covering scope of the present invention.
Claims (8)
- A kind of 1. method for propagating Monochamus alternatus artificially, it is characterised in that methods described includes as follows：（1）The artificial feeding of the sub- ripe individual of Monochamus alternatus；（2）The outfit in the artificial delivery room of Monochamus alternatus；（3）The artificial pairing raising and induction breeding of Monochamus alternatus sexal maturity individual；（4）The preparation of each instar larvae man-made feeds of Monochamus alternatus；（5）The separation of the nascent larva of Monochamus alternatus obtains；（6）Alternatus Larva is transferred to the follow-up cultivation of man-made feeds；（7）Monochamus alternatus pupa time and management of sprouting wings.
- A kind of 2. method for propagating Monochamus alternatus artificially according to claim 1, it is characterised in that：Step（1）For：In pine Black longicorn generation area pine forest suspension Monochamus alternatus trap trapping wild Monochamus alternatus live body；Or separated from epidemic disease wood aging Alternatus Larva, take 12 milliliters of EP test tubes, insert natural wood pulp paper handkerchief, add water to soak, by aging alternatus Larva Temperature is positioned over as 25 ~ 28 DEG C, the constant temperature of humidity 65 ~ 80% as in this pipe, and by the test tube equipped with Monochamus alternatus mature larva Dark culturing in case, promote mature larva to pupate and finally sprout wings into adult.
- A kind of 3. method for propagating Monochamus alternatus artificially according to claim 1, it is characterised in that：The Monochamus alternatus people The outfit in work delivery room：It is respectively delivery room of 50 ~ 60CM, a height of 80 ~ 120CM the grenadine cage as longicorn to prepare length and width, in cage Put a 500ML triangular flasks, injected clear water, using water planting in fresh pine tree branch insertion triangular flask as nutrition branch in center； An a diameter of 5 ~ 10CM is respectively placed in corner in cage, length is 15 ~ 20CM and the fresh healthy masson pine belt leather of sealing two ends Juggle is as spawning wood；By above-mentioned Monochamus alternatus delivery room be placed in temperature be 25 ~ 28 DEG C, humidity be 65 ~ 80%, photoperiod be illumination： Dark=12:In 12h greenhouse.
- A kind of 4. method for propagating Monochamus alternatus artificially according to claim 1, it is characterised in that：Monochamus alternatus sexal maturity The artificial pairing of individual is raised and induction breeding is：Monochamus alternatus imago is male for one group according to two female one, it is put into ready Delivery room, allow its panmixia, spawning；Each delivery room is put into five to six group Monochamus alternatus imagos；One time of nutrition is changed every three days Branch and spawning wood, and time mark is carried out on spawning wood.
- A kind of 5. method for propagating Monochamus alternatus artificially according to claim 1, it is characterised in that：The nascent children of Monochamus alternatus After the spawning wood that the separation of worm is retrieved as to change in delivery room is placed in the greenhouse of the same terms and continues culture 12 ~ 15 days, Carefully bark and xylem are peeled off with woodcut knife, take out the nascent alternatus Larva stayed and be born in bast.
- A kind of 6. method for propagating Monochamus alternatus artificially according to claim 1, it is characterised in that：Alternatus Larva turns The follow-up cultivation for entering man-made feeds is：The bark for the spawning wood for isolating alternatus Larva and xylem are crushed to four respectively Below ten mesh, xylem powder, bark fines are prepared into, it is standby；Monochamus alternatus low instar larvae feed of the assignment configuration into 1 ~ 2 age With the Monochamus alternatus advanced age larvae feeds after 3 ages；Use in feed center in the centrifuge tube of the low instar larvae feed dispensed The tweezers of sterilization prick an aperture, and isolated nascent alternatus Larva head is lowered into aperture, covers centrifuge tube Lid, is placed in being cultivated in delivery room environmental condition identical growth cabinet；The feeding situation of routine observation alternatus Larva, two Carefully the alternatus Larva at 3 initial stages in age that grew to is taken out after week, completes the raising in low age stage, afterwards for above-mentioned same Method the alternatus Larva of taking-up be transferred in middle advanced age larvae feeds carry out follow-up cultivation, routine observation Monochamus alternatus Feeding situation and growing state, until Monochamus alternatus man-made feeds in it is smooth go out pupa room pupate, finally sprout wings a new generation Adult.
- A kind of 7. method for propagating Monochamus alternatus artificially according to claim 6, it is characterised in that：1 ~ 2 described age Monochamus alternatus low instar larvae feed includes following raw material：45 ~ 55 parts of xylem powder, 90 ~ 110 parts of bark fines are beautiful 60 ~ 70 parts of one or more in ground rice, soy meal, wheat bran, edible fungus bran, benzoic acid, sodium benzoate, sorbic acid, sorb 2 ~ 3 parts of one or more in sour potassium, dry ferment, dusty yeast, 20 ~ 30 parts of the one or more of yeast extract kind, agar strip 25 ~ 35 parts, 8 ~ 15 parts of shrimp shell meal, the Fen , Unit of sucrose 35 ~ 45 steam 280 ~ 320 parts of water；Monochamus alternatus advanced age after 3 described ages Larvae feeds include following raw material：140 ~ 160 parts of xylem powder, corn flour, soy meal, wheat bran, edible fungus bran In 60 ~ 70 parts of one or more, 2 ~ 3 parts of one or more in benzoic acid, sodium benzoate, sorbic acid, potassium sorbate, dry ferment Mother, dusty yeast, 20 ~ 30 parts of the one or more of yeast extract kind, 25 ~ 35 parts of agar strip, 8 ~ 15 parts of shrimp shell meal, sucrose 35 ~ It is 45 parts, single to steam 280 ~ 320 parts of water.
- A kind of 8. method for propagating Monochamus alternatus artificially according to claim 1, it is characterised in that：Monochamus alternatus pupa time and Emergence management is：The adult newly to sprout wings is put into single cage in raising box to raise 7 ~ 10 days, delivery room mating, spawning is transferred to, completes one Wheel raising；It is by the operation in batches of Monochamus alternatus imago, the growth phase of Monochamus alternatus is interleaved, reach while possess each worm The Monochamus alternatus of state and growth phase；Constantly circulation above procedure, realize the laboratory artificial cultivating of Monochamus alternatus.
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| CN108283253A (en) * | 2017-12-08 | 2018-07-17 | 重庆乾富科技有限公司 | A kind of feed and preparation method thereof of the bent tooth prionid larva of cultivation |
| CN109122596A (en) * | 2018-09-29 | 2019-01-04 | 中国林业科学研究院资源昆虫研究所 | A method of roundheaded borer is cultivated using agricultural crop straw |
| CN109349233A (en) * | 2018-09-29 | 2019-02-19 | 中国林业科学研究院资源昆虫研究所 | A method of roundheaded borer is cultivated using discarded bacterium bag |
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| CN108283253A (en) * | 2017-12-08 | 2018-07-17 | 重庆乾富科技有限公司 | A kind of feed and preparation method thereof of the bent tooth prionid larva of cultivation |
| CN110692600A (en) * | 2018-07-10 | 2020-01-17 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial feed for anoplophora chinensis larvae and subculturing method of anoplophora chinensis |
| CN110692600B (en) * | 2018-07-10 | 2022-04-19 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial feed for anoplophora chinensis larvae and subculturing method of anoplophora chinensis |
| CN109122596A (en) * | 2018-09-29 | 2019-01-04 | 中国林业科学研究院资源昆虫研究所 | A method of roundheaded borer is cultivated using agricultural crop straw |
| CN109349233A (en) * | 2018-09-29 | 2019-02-19 | 中国林业科学研究院资源昆虫研究所 | A method of roundheaded borer is cultivated using discarded bacterium bag |
| CN109601488A (en) * | 2018-12-28 | 2019-04-12 | 中国林业科学研究院资源昆虫研究所 | Utilize the method for sawdust cultivation roundheaded borer |
| CN111513028A (en) * | 2020-06-19 | 2020-08-11 | 广西大学 | Efficient establishing method and feeding device for experimental population of Sucus bubali |
| CN113142143A (en) * | 2021-04-19 | 2021-07-23 | 福建农林大学 | Method for breaking diapause of Monochamus alternatus diapause larvae |
| CN113907051A (en) * | 2021-12-03 | 2022-01-11 | 遵义市林业科学研究所 | Artificial breeding method of zanthoxylum bungeanum and tiger longicorn |
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