CN102715377A - Formula of feed for cultivating monochamus alternatus and preparation process - Google Patents

Formula of feed for cultivating monochamus alternatus and preparation process Download PDF

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Publication number
CN102715377A
CN102715377A CN2012102205952A CN201210220595A CN102715377A CN 102715377 A CN102715377 A CN 102715377A CN 2012102205952 A CN2012102205952 A CN 2012102205952A CN 201210220595 A CN201210220595 A CN 201210220595A CN 102715377 A CN102715377 A CN 102715377A
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monochamus alternatus
cultivating
feed
monochamus
feed formula
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林海萍
黄俊浩
郭恺
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Zhejiang A&F University ZAFU
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Zhejiang A&F University ZAFU
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Abstract

The invention provides a formula of a feed for cultivating monochamus alternatus and a preparation process and belongs to the technical field of feed processing. The formula of the feed for cultivating the monochamus alternates is characterized by containing deal powder 37-55%, fungus 2-9%, nutritional ingredients 3-11%, auxiliary materials 8-21% and a solvent 13-39% and contains various nutrient substance required by the monochamus alternates for growth and development, and the monochamus alternates which is unobviously different from monochamus alternates growing in the natural world can be cultivated artificially in a monochamus alternates laboratory. In addition the cultivating period can be shortened, the yield and the quality of the monochamus alternates can be ensured, and the formula of the feed is good in effect, simple and convenient to manufacture, safe, effective, low in cost and capable of meeting the requirements for artificial cultivation of the scientific research laboratory.

Description

A kind of feed formula and preparation technology who cultivates Monochamus alternatus
Technical field
The invention belongs to the feed processing technology field, be specifically related to a kind of feed formula and preparation technology who cultivates Monochamus alternatus.
Background technology
Monochamus alternatus is to propagate the topmost insect vector of pine wood nematode, and carrying rate is 62.5%, and on average the amount of carrying 4601-18445 bar/head reaches as high as 289000/head.The longicorn of harm Pinus has 6 kinds: Monochamus alternatus, little grey psacotheahilaris, brown stalk longicorn, red short stalk longicorn, vertebra longicorn and Song Pi ridge flower longicorn.Preceding 5 kinds of carrier that longicorn is a pine wood nematode have been proved at present.
Monochamus alternatus is got tonic is filled becomes pine wood nematode in the nutritive process communication media, and every pine wood nematode spot is found in investigation, nearly all has Monochamus alternatus to exist.Ding Yuzhou etc. discover that Monochamus alternatus population density and pine wood nematode occurring degree are proportionate, and therefore prevent and treat longicorn, and reducing insect density is prevention and the fundamental way of wiping out and treating insect pests and plant diseases pine nematode.Friend gold is reluctant to fly with thinking kind the flying of Monochamus alternatus, seldom flight after the emergence, natural diffusivity is not strong, in pure forest scope of activities in 100m, general 1 generation adult outwards be diffused in about 150m.But come Yan Xue etc. to think that Monochamus alternatus can be the most important origin mechanism in the new epidemic place of pine nematode with the air-flow long range diffusion; Must cause preventing and controlling person's great attention; And further research thinks that Monochamus alternatus is the main biotic factor that causes that pine tree is withered; In pine tree is withered, brought into play main effect, its harmfulness is considerably beyond pine wood nematode.Coming swallow to learn through the experiment confirm Monochamus alternatus is the one of the main reasons that causes that pine tree is withered, and preventing and treating Monochamus alternatus energetically is the key of protection China pine forest.The pine wood nematode volume is little; Be grown in the pin main body, can not self-dependent activity infect new host, can not have the ability of propagating with air-flow, rainwater as microorganisms such as fungi, bacteriums; In addition; Generally do not advance people's soil yet, in soil, shift the possibility of propagating very little (when having only trees ' root injured, pine wood nematode just can be invaded people's root from soil).So,, also just controlled pine nematode indirectly as long as control communication media-Monochamus alternatus.At present, the study on prevention of pine nematode is mainly carried out to Monochamus alternatus, hopes to cut off its route of transmission, reduces the population density of its communication media insect Monochamus alternatus effectively.Therefore prevent and treat Monochamus alternatus, cut off pine wood nematode to infect circulation be the major technique in the pine nematode comprehensive regulation.
For protection accounts for nearly half the pine forest health of area of woods, avoid causing society, ecology and the economic loss that can't retrieve because of pine wood nematode, should pay attention to the research and the control of Monochamus alternatus.The Monochamus alternatus laboratory cultures is a difficult problem always.The present invention relates generally to Monochamus alternatus and carries out the feed difficult problem in the ARTIFICIAL CULTURE; Solve the difficult present situation of current Monochamus alternatus laboratory ARTIFICIAL CULTURE; Be intended to seek easy, effective, stable indoor cultivation feed formula, for further culture studies and control Monochamus alternatus provide safeguard.
Summary of the invention
To the problems referred to above that exist in the prior art, the objective of the invention is to design provides a kind of feed formula of Monochamus alternatus and technical scheme of preparation technology of cultivating, its safety non-toxic, and simple and effective, easy to use, with low cost, feeding effect is good.
Described a kind of feed formula of cultivating Monochamus alternatus is characterized in that containing the following material of weight percentage: pine powder 37-55%, mushroom 2-9%, nutritional labeling 3-11%, auxiliary material 8-21%, solvent 13-39%;
Described mushroom is one or more mixtures in saccharomycete, Bacillus acidi lactici, saddle fungus, peptostreptococcus asaccharolyticus, matsutake, the bolete; Described nutritional labeling is one or more mixtures in glycine, Sodium Benzoate, sorbic acid, ammonium sulfate, sodium dihydrogen phosphate, sodium acid carbonate, the sodium acetate; Described auxiliary material is one or more mixtures in fructose, lactose, glucose, sucrose, phosphoric acid, sulfuric acid, hydrochloric acid, glacial acetic acid, formic acid, agar, xylitol, Arabic gum, the peach gum; Described solvent is one or more mixtures in distilled water, the mineral water.
Described a kind of feed formula of cultivating Monochamus alternatus is characterized in that the content of said pine powder is 39-53%, is preferably 41-50%.
Described a kind of feed formula of cultivating Monochamus alternatus, the content that it is characterized in that said mushroom is 4-8%, preferred 5-7%.
Described a kind of feed formula of cultivating Monochamus alternatus is characterized in that the content of said nutritional labeling is 4-10%, is preferably 6-9%.
Described a kind of feed formula of cultivating Monochamus alternatus is characterized in that the content of said auxiliary material is 10-19, is preferably 13-18%.
Described a kind of feed formula of cultivating Monochamus alternatus is characterized in that the content of said solvent is 15-37, is preferably 16-35%.
Described a kind of preparation technology who cultivates the feed formula of Monochamus alternatus is characterised in that may further comprise the steps:
1) take by weighing pine powder, mushroom, nutritional labeling, auxiliary material, solvent according to the said ratio of claim 1, subsequent use;
2) mixture after above-mentioned raw materials is stirred, all making beating is crushed to the 100-200 order, promptly gets the feed of Monochamus alternatus.
Above-mentioned a kind of feed formula and preparation technology who cultivates Monochamus alternatus contains the Monochamus alternatus required various nutriments that grow, and can on the ARTIFICIAL CULTURE of Monochamus alternatus laboratory, raise out and the occurring in nature growth does not have the obviously Monochamus alternatus of difference; Simultaneously can shorten cultivation cycle; Guarantee the output and the quality of Monochamus alternatus, this feed formula feeding effect is good and simple for production, safe and effective; With low cost, can be in the demand that satisfies on the ARTIFICIAL CULTURE of scientific experiment chamber.
The percentage composition that relates in the present specification unless otherwise indicated, other the weight percentage that is pure material.
The specific embodiment
Below through specific embodiment the present invention is described further, and combine the test of pesticide effectiveness to further specify beneficial effect of the present invention.
Embodiment 1
In the 500L agitated reactor, drop into 175kg distilled water; Drop into the 205kg pine powder again, add 5kg Bacillus acidi lactici, 5kg saddle fungus, 5kg peptostreptococcus asaccharolyticus, 5kg matsutake and 5kg bolete then while stirring, slowly drop into 6kg sorbic acid, 6kg ammonium sulfate, 6kg sodium dihydrogen phosphate, 6kg sodium acid carbonate and 6kg sodium acetate again; Stir half an hour; Mix, add 5kg glucose, 6kg sucrose, 6kg phosphoric acid, 6kg sulfuric acid, 6kg hydrochloric acid, 6kg glacial acetic acid, 6kg formic acid, 6kg agar, 6kg xylitol, 6kg Arabic gum and 6kg peach gum at last, the mixture after the heated and stirred dissolving fully; Making beating is crushed to 100 orders, final products.
Embodiment 2
In the 500L agitated reactor, drop into the 80kg mineral water; Drop into the 150kg pine powder again, add 5kg saccharomycete, 10kg peptostreptococcus asaccharolyticus, 10kg matsutake and 10kg bolete then while stirring, slowly drop into 5kg glycine, 10kg Sodium Benzoate, 10kg sorbic acid, 10kg ammonium sulfate, 3kg sodium dihydrogen phosphate, 3kg sodium acid carbonate and 4kg sodium acetate again; Stir half an hour; Mix, add 9kg fructose, 9kg lactose, 9kg sulfuric acid, 9kg hydrochloric acid, 9kg glacial acetic acid, 9kg formic acid, 9kg agar, 9kg xylitol, 9kg Arabic gum and 9kg peach gum at last, the mixture after the heated and stirred dissolving fully; Making beating is crushed to 200 orders, final products.
Embodiment 3
In the 500L agitated reactor, drop into 90kg distilled water and 69kg mineral water; Drop into the 210kg pine powder again, add 7kg saccharomycete, 10kg Bacillus acidi lactici, 5kg saddle fungus and 5kg bolete then while stirring, slowly drop into 4kg glycine, 10kg Sodium Benzoate, 10kg sodium dihydrogen phosphate, 5kg sodium acid carbonate and 5kg sodium acetate again; Stir half an hour; Mix, add 5kg glucose, 5kg sucrose, 10kg phosphoric acid, 10kg formic acid, 10kg agar, 10kg xylitol, 10kg Arabic gum and 10kg peach gum at last, the mixture after the heated and stirred dissolving fully; Making beating is crushed to 120 orders, final products.
Embodiment 4
In the 500L agitated reactor, drop into 138kg distilled water; Drop into the 220kg pine powder again, add 9kg saccharomycete, 4kg Bacillus acidi lactici, 4kg saddle fungus, 4kg peptostreptococcus asaccharolyticus, 4kg matsutake and 4kg bolete then while stirring, slowly drop into 8kg glycine, 5kg Sodium Benzoate, 5kg sorbic acid, 10kg ammonium sulfate and 10kg sodium acetate again; Stir half an hour; Mix, add 8kg fructose, 8kg glucose, 8kg sucrose, 8kg phosphoric acid, 8kg sulfuric acid, 5kg hydrochloric acid, 10kg glacial acetic acid, 10kg Arabic gum and 10kg peach gum at last, the mixture after the heated and stirred dissolving fully; Making beating is crushed to 140 orders, final products.
Embodiment 5
In the 500L agitated reactor, drop into the 117kg mineral water; Drop into the 230kg pine powder again, add 11kg saccharomycete, 5kg Bacillus acidi lactici, 5kg saddle fungus, 5kg peptostreptococcus asaccharolyticus and 5kg matsutake then while stirring, slowly drop into 2kg glycine, 10kg Sodium Benzoate, 10kg sorbic acid, 10kg ammonium sulfate and 10kg sodium dihydrogen phosphate again; Stir half an hour; Mix, add 5kg fructose, 5kg lactose, 5kg glucose, 5kg sucrose, 5kg phosphoric acid, 5kg sulfuric acid, 10kg hydrochloric acid, 10kg glacial acetic acid, 10kg formic acid, 10kg agar and 10kg xylitol at last, the mixture after the heated and stirred dissolving fully; Making beating is crushed to 160 orders, final products.
Embodiment 6
In the 500L agitated reactor, drop into 99kg distilled water; Drop into the 240kg pine powder again, add 3kg saccharomycete, 10kg saddle fungus, 10kg matsutake and 10kg bolete then while stirring, slowly drop into 3kg Sodium Benzoate, 10kg sorbic acid, 10kg ammonium sulfate, 10kg sodium dihydrogen phosphate and 10kg sodium acetate again; Stir half an hour; Mix, add 5kg fructose, 10kg lactose, 10kg glucose, 10kg phosphoric acid, 10kg sulfuric acid, 10kg hydrochloric acid, 10kg glacial acetic acid, 10kg xylitol and 10kg peach gum at last, the mixture after the heated and stirred dissolving fully; Making beating is crushed to 180 orders, final products.
The excellent results of the feed formula of above-mentioned Monochamus alternatus below is described through test.
Figure 2012102205952100002DEST_PATH_IMAGE001
Table 1 shows: embodiment 1-6 all has effect preferably to the raising of Monochamus alternatus, and embodiment 2 is superior to embodiment 1, and embodiment 3 is superior to embodiment 4, and embodiment 6 is superior to embodiment 5.
Figure 2012102205952100002DEST_PATH_IMAGE002
Table 2 shows: hatching all has effect preferably to embodiment 1-6 to the pine wood nematode ovum, and embodiment 2 is superior to embodiment 1, and embodiment 4 is superior to embodiment 3, and embodiment 6 is superior to embodiment 5, with control group significant difference is arranged all.

Claims (7)

1. feed formula of cultivating Monochamus alternatus is characterized in that containing the following material of weight percentage: pine powder 37-55%, mushroom 2-9%, nutritional labeling 3-11%, auxiliary material 8-21%, solvent 13-39%;
Described mushroom is one or more mixtures in saccharomycete, Bacillus acidi lactici, saddle fungus, peptostreptococcus asaccharolyticus, matsutake, the bolete; Described nutritional labeling is one or more mixtures in glycine, Sodium Benzoate, sorbic acid, ammonium sulfate, sodium dihydrogen phosphate, sodium acid carbonate, the sodium acetate; Described auxiliary material is one or more mixtures in fructose, lactose, glucose, sucrose, phosphoric acid, sulfuric acid, hydrochloric acid, glacial acetic acid, formic acid, agar, xylitol, Arabic gum, the peach gum; Described solvent is one or more mixtures in distilled water, the mineral water.
2. a kind of feed formula of cultivating Monochamus alternatus as claimed in claim 1 is characterized in that the content of said pine powder is 39-53%, is preferably 41-50%.
3. a kind of feed formula of cultivating Monochamus alternatus as claimed in claim 1, the content that it is characterized in that said mushroom is 4-8%, preferred 5-7%.
4. a kind of feed formula of cultivating Monochamus alternatus as claimed in claim 1 is characterized in that the content of said nutritional labeling is 4-10%, is preferably 6-9%.
5. a kind of feed formula of cultivating Monochamus alternatus as claimed in claim 1 is characterized in that the content of said auxiliary material is 10-19, is preferably 13-18%.
6. a kind of feed formula of cultivating Monochamus alternatus as claimed in claim 1 is characterized in that the content of said solvent is 15-37, is preferably 16-35%.
7. a kind of preparation technology who cultivates the feed formula of Monochamus alternatus as claimed in claim 1 is characterised in that may further comprise the steps:
1) take by weighing pine powder, mushroom, nutritional labeling, auxiliary material, solvent according to the said ratio of claim 1, subsequent use;
2) mixture after above-mentioned raw materials is stirred, all making beating is crushed to the 100-200 order, promptly gets the feed of Monochamus alternatus.
CN2012102205952A 2012-06-29 2012-06-29 Formula of feed for cultivating monochamus alternatus and preparation process Pending CN102715377A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103349183A (en) * 2013-07-17 2013-10-16 天津拓华生物科技有限责任公司 Feed for artificially feeding thyestilla gebleri adults and application thereof
CN107156543A (en) * 2017-05-10 2017-09-15 福建农林大学 A kind of man-made feeds of Monochamus alternatus low instar larvae and preparation method thereof
CN107410213A (en) * 2017-05-10 2017-12-01 福建农林大学 A kind of method for propagating Monochamus alternatus artificially

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101228853A (en) * 2007-01-23 2008-07-30 中国林业科学研究院森林生态环境与保护研究所 Artificial feeding and culture transfer of monochamus alternatus hope and artificial feed for larva thereof
CN101983580A (en) * 2010-10-25 2011-03-09 南京林业大学 Forage for artificial breeding of Monochamus alternatus Hope and method for preparing the same
CN102217729A (en) * 2011-04-26 2011-10-19 福建省林业科学研究院 Semi-artificial feed for anoplophora chinensis larvae and artificial feeding method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101228853A (en) * 2007-01-23 2008-07-30 中国林业科学研究院森林生态环境与保护研究所 Artificial feeding and culture transfer of monochamus alternatus hope and artificial feed for larva thereof
CN101983580A (en) * 2010-10-25 2011-03-09 南京林业大学 Forage for artificial breeding of Monochamus alternatus Hope and method for preparing the same
CN102217729A (en) * 2011-04-26 2011-10-19 福建省林业科学研究院 Semi-artificial feed for anoplophora chinensis larvae and artificial feeding method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103349183A (en) * 2013-07-17 2013-10-16 天津拓华生物科技有限责任公司 Feed for artificially feeding thyestilla gebleri adults and application thereof
CN107156543A (en) * 2017-05-10 2017-09-15 福建农林大学 A kind of man-made feeds of Monochamus alternatus low instar larvae and preparation method thereof
CN107410213A (en) * 2017-05-10 2017-12-01 福建农林大学 A kind of method for propagating Monochamus alternatus artificially

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Application publication date: 20121010