CN109548564A - A kind of pure artificial cultivation new method of termite series collybia albuminosa - Google Patents
A kind of pure artificial cultivation new method of termite series collybia albuminosa Download PDFInfo
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- CN109548564A CN109548564A CN201910022658.5A CN201910022658A CN109548564A CN 109548564 A CN109548564 A CN 109548564A CN 201910022658 A CN201910022658 A CN 201910022658A CN 109548564 A CN109548564 A CN 109548564A
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- 241000222680 Collybia Species 0.000 title claims abstract description 91
- 238000000034 method Methods 0.000 title claims abstract description 35
- 238000009395 breeding Methods 0.000 claims abstract description 87
- 230000001488 breeding effect Effects 0.000 claims abstract description 87
- 230000008569 process Effects 0.000 claims abstract description 10
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- 239000002689 soil Substances 0.000 claims description 26
- 241000894006 Bacteria Species 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 9
- 239000008188 pellet Substances 0.000 claims description 9
- 238000005286 illumination Methods 0.000 claims description 7
- 235000016709 nutrition Nutrition 0.000 claims description 7
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- 230000000694 effects Effects 0.000 description 3
- 239000004744 fabric Substances 0.000 description 3
- 241001411320 Eriogonum inflatum Species 0.000 description 2
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
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- Animal Behavior & Ethology (AREA)
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- Biodiversity & Conservation Biology (AREA)
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Abstract
The present invention relates to a kind of pure artificial cultivation new methods of termite series collybia albuminosa, by selecting fine quality, restore the wild condition of collybia albuminosa, make full use of the symbiosis of collybia albuminosa and termite, realize collybia albuminosa large-scale plantation, under the management of science, the excellent condition of breeding and existence is created for collybia albuminosa termite, improve the breeding potential of collybia albuminosa breeding termite, solves critical issue for artificial cultivation collybia albuminosa, yield is greatly improved, stay in grade and uniformly, the collybia albuminosa nutritive value and medical value planted out are high, flavor taste is good, method to obtain large-scale breeding collybia albuminosa, realize artificial cultivation collybia albuminosa.The conditions such as subenvironment temperature, the humidity grown by manual adjustment collybia albuminosa, make its few term also can fruiting, realize anti-season listing, to further increase its economic benefit;It ensure that product quality so that collybia albuminosa is grown in organic environment without using any chemical agent in planting process, reduce planting cost.
Description
Technical field
The invention belongs to wild mushroom artificial propagation techniques fields, and in particular to a kind of pure Planting of termite series collybia albuminosa
Train new method.
Background technique
Collybia albuminosa is one of four big bacterium, is treasure in edible wild bacterium.Collybia albuminosa meat thickness is big and fleshy, and matter filament is white, taste
The fresh and sweet delicious and crisp in road.Containing amino acid, protein necessary to human body, fat, also contain various vitamins and calcium, phosphorus, core yellow acid etc.
Substance.Collybia albuminosa can pure be dish, moreover it is possible to it arranges in pairs or groups with vegetables, the flesh of fish and various all kinds of delicacies, no matter fry, fry, salting down, decocting, mixing,
It is braised, roasting, stewing, it steams in clear soup or cooks soup, taste is all very fresh, is the hat in bacterium.Containing a kind of rare composition ergot in collybia albuminosa
Sterol is adjustable the balance of human hormone if human body supplements this substance in right amount, prevents the generation of a variety of chronic diseases.Chicken fir
Bacterium only southwest, a few provinces in the southeast and Taiwan some areas produce, price is higher due to its wild low output.
Collybia albuminosa not only has nutritive value abundant, while also having very high medical value, have beneficial stomach, clear mind,
The effects of controlling hemorrhoid and reducing blood lipid, also have enriching the blood to restore normal menstruation, strengthening the spleen and stomach and other effects, can be used for treating loss of appetite, endless diarrhea,
The all diseases of blood under hemorrhoid are traditional one of the medicinal fungis in China.
The growth mechanism of collybia albuminosa and most wild mushrooms has huge difference, and physiological property is very special, and collybia albuminosa is certainly
Body will not be bred, and entire breeding and growth cycle will rely on termite in the soil, in long-term evolutionary process gradually and termite
Evolving is the symbiosis ecosystem, and termite propagates the basidiospore and mycelium of collybia albuminosa, and cultured mycelia, also feeding is turned out
Collybia albuminosa bulb forms also be unable to do without the secretion of termite as food, the growth and development of another aspect collybia albuminosa bulb forms,
The secretion collybia albuminosa for having left termite is just difficult to survive, the two mutual reciprocity and mutual benefit, and commensalism is all total in an environment, group
With development, such ecological environment causes collybia albuminosa to the characteristic requirements of growth conditions, also Just because of this, collybia albuminosa breeding,
Growth rhythm till now there are no generally acknowledged result of study, just do not know where to begin yet by the artificial growth of collybia albuminosa.The fungus garden evidence of termite
Measurement contains more than 20 kinds of amino acid, and collybia albuminosa can just show unique flavor just because of in this grown on matrix, and use biography
The collybia albuminosa that the cultivation method for edible mushroom of system is cultivateeed out, due to the change of its growth substrate and growing environment, collybia albuminosa
Nutritive value, flavor, medical value can all have a greatly reduced quality.
Summary of the invention
In order to solve the above problems existing in the present technology, the present invention provides a kind of the pure artificial of termite series collybia albuminosa
New method for cultivating.It by restoring the wild environment of collybia albuminosa to greatest extent, and carries out rationalizing dense planting and management, has reached realization
Collybia albuminosa artificial growth greatly improves its yield under the premise of guaranteeing initial quality.
The technical scheme adopted by the invention is as follows:
A kind of pure artificial cultivation new method of termite series collybia albuminosa, includes the following steps:
(1) culture utensil is chosen, being laid with one layer of compost to culture utensil bottom, (compost should not press too real and too
Pine), compost moisture content is 15.5%-19.5%, one porous plate of laying above the compost, same on the porous plate
The Nutrition Soil of plate hole axis setting multi-turn and be distributed in a ring, corresponding each plate hole locations draws cave depth 5-10mm, diameter 4-5mm;
Multiple annular bulkheads are also coaxially disposed in the top of the porous plate, the annular bulkhead is located in the gap in different girth sheets holes
Between;The distance between adjacent plate hole positioned at same circle is equal, positioned at same circle multiple plate holes to adjacent annular bulkhead away from
From being equal;
(2) breeding termite is collected at different collybia albuminosa cavities mouths, dealation processing is carried out to breeding termite, after obtaining dealation
Breeding termite;
(3) above culture utensil, in the annular interface being located between two adjacent annular bulkheads, uniformly investment is shaken
The input amount of breeding termite after dealation, the breeding termite is twice of the annular interface upper plate number of perforations, described in holding
Cultivating the air humidity in utensil is 80%-90%, and the culture soil's water content is 15.5%-19.5%, and temperature is in 25-28
DEG C, culture breeding termite transplants the cave chamber formicary for the termite to reach maturity occur up to there is the termite to reach maturity respectively
It is cultivated in multiple independent utensils, and is covered with the ant of collybia albuminosa mycelium pellet in the intracavitary dispensing in the cave of the cave chamber formicary
Nest also adds spare dry ant nest the cave of the cave chamber formicary is intracavitary in the management process, after the completion of breeding, is formed multiple
The ant nest bacterium nest of forming;
(4) one layer of compost (compost should not press too real and too loose) is laid on concrete floor, in the culture
Formicary is uniformly excavated on soil, is later integrally dug out step (3) the ant nest bacterium nest respectively, and be implanted into the formicary respectively
In, the formicary is covered with the compost of 5-15cm thickness, cultivates fruiting;During management of producing mushroom, the temperature in formicary is first controlled
It is 13-20 DEG C, after being kept for 3-4 months, then controlled at 15-28 DEG C until management fruiting, holding culture soil's water content are
15.2%-19.5%.
In step (1), incubator bottom be laid with compost with a thickness of 2-20cm.
In step (1), the plate hole on the porous plate is uniformly distributed, and the aperture of each plate hole is 4- on the porous plate
5mm, the distance between adjacent aperture centers be 2-5m, the porous plate with a thickness of 1-20mm;Peripheral frame on the peripheral frame side wall
The aperture in hole is 4-5mm.
Several perpendicular bulkheads are set between two neighboring annular bulkhead, the center of each perpendicular bulkhead is respectively provided with string hole,
Two plate holes are arranged between two neighboring perpendicular bulkhead, and two plate holes are located at different circles;The purpose being arranged in this way is to avoid breeding
Ant is for a long time and remote movable in annular interface.Preferably, the perpendicular bulkhead is arranged on the middle line of two neighboring plate hole.
In step (1), the compost first carries out high-temperature sterilization;
Two girth sheets holes are set between each adjacent two annular bulkhead, and the thickness of the multiple annular bulkhead is 0.006-
1mm。
In step (2), the concrete operations that breeding termite is collected at the collybia albuminosa cavities mouth are as follows: Xiang Suoshu collybia albuminosa
Cavities mouth injects the compressed gas that humidity is 80-90%, and control intensity of illumination is by gradually dying down by force or gradually becoming strong, air pressure by weak
By gradually dying down by force or gradually becoming strong by weak, temperature is at 25-28 DEG C.It is collected and is induced using equipment, the control gentle pressure of illumination is weak
Effect is optimum state, and breeding termite is promoted to climb out of and collect out of hole.
In step (3), the separate device for carrying out the breeding culture is culture bottle.The ant for being covered with collybia albuminosa mycelium pellet
The diameter of nest is 0.5-1cm.
In step (3), the diameter of the ant nest for being covered with collybia albuminosa mycelium pellet is 1-1.5cm.
In step (3), the time of the breeding is 30-45 days.
In step (4), the compost with a thickness of 1-1.5m.
In step (4), nest the depth 45-55cm, diameter 30-50cm of the formicary.
In step (4), cultivate in the management process of fruiting, it is also thin to dispensing above the compost of the correspondence formicary position
Sawdust and dry weeds.
As the embodiment that can choose, it can according to need the size for expanding annular bulkhead or extend adjacent plate hole
The distance between hole center, when the aperture of plate hole each on the porous plate is that the distance between 4-5mm, adjacent aperture centers are
Annular interface when two girth sheets holes is set between 1-3cm, each adjacent two annular bulkhead, between two adjacent annular bulkheads
The upper quantity for launching breeding termite is twice for being the annular interface upper plate number of perforations, and the Nutrition Soil of each plate hole locations draws cave
Deep 5-10mm, diameter 4-5mm;The outside wall surface of annular bulkhead and the outside wall surface of porous plate be set as it is smooth be unfavorable for breed termite climb
Row.
It should be noted that being handled from breeding termite is collected to dealation, then cultivated in launching to culture utensil, the time gets over
Fast better, ambient air humidity 80%-90%, temperature have the air-flow environment of 2-3 grades of wind scale that can make to breed at 25-28 DEG C
Termite is more comfortable, their advantageous normal activities, and environment temperature is excessively high, humidity is too low can be such that breeding termite dysphoria strives
It stings, dispensing processing their longer mobilities of time interval after dealation processing or dealation are lower.
Furthermore it is also possible to divide group to breed for termite is bred: the breeding termite of same collybia albuminosa nest is collected in a bottle
It is trained ant king and queen places into culture in culture utensil.Specifically: the bottle wall of bottle is equipped with multiple breeding termites and cannot climb
Aperture out, to prevent breeding termite from escaping.Aperture is used to mud layer moisture content in regulating and controlling bottle, applies soil when use in aperture
Aperture is filled up, bottle one end is equipped with the bottleneck of diameter 3cm, and bottleneck has a bottle stopper, the bottle other end be equipped with it is multiple can make soldier ant,
The bar shaped aperture that worker ant climbs out of, bottle inner wall stud with the sheet glass of multiple thickness 2cm, diameter 15cm, and the multiple sheet glass is put into
Annular, each sheet glass and bottle inner wall are in 90 ° of angles, and the edge requirements of sheet glass do not stay cutting edge, and the glass plate plane is mutually flat
It goes, the vertical distance between adjacent glass sheets parallel surface is 1.5cm.
When collecting breeding termite, the spare bottle stopper of bottle is opened, the moisture content that suitable termite utilizes is painted into bottle and is existed
50% acid red mud, bottleneck is leaned against on the ant hole of collybia albuminosa nest, and the ant hole of collybia albuminosa nest is gradually reduced at setting in bottle
Volume, reduce every three days it is primary, by collybia albuminosa nest wait fly there is wing breeding termite mud cave to reduce 1/5 every time, to promote to breed
Termite crawls into the center pocket ant that breeding termite is formed in bottle and in bottle, and bottle is equipped with for observing the window for breeding termite in bottle
Mouthful, until the inside of the window is accumulated by breeding termite with mud, or when weather is suitble to, breeding termite will climb from hole
It flies away out, or sees that breeding termite wing has breakage from the window, indicate that breeding termite reaches maturity, at this moment breeding
It is placed again into this bottle after ant artificial dealation processing or after making their natural dealations, to be trained ant king and queen, described in completion
Divide group's reproductive process.
The breeding ants that culture is launched in addition find that more are gently dispersed in the available disinfection brush that a hole burrows in groups
Reach male female each one ant colony breeding and improves breeding potential.
The invention has the benefit that
The pure artificial cultivation new method of termite series collybia albuminosa of the present invention, by selecting fine quality, also jungle fowl
The wild condition of fir bacterium makes full use of the symbiosis of collybia albuminosa and termite, realizes collybia albuminosa large-scale plantation, in the pipe of science
Under reason, the excellent condition of breeding and existence is created for collybia albuminosa termite, improves the breeding potential of collybia albuminosa breeding termite, is people
Work cultivation collybia albuminosa solves critical issue, and yield is greatly improved, stay in grade and uniformly, the collybia albuminosa battalion planted out
It supports value and medical value is high, flavor taste is good, so that the method for obtaining large-scale breeding collybia albuminosa, realizes artificial cultivation chicken fir
Bacterium.The conditions such as subenvironment temperature, the humidity grown by manual adjustment collybia albuminosa, make its few term also can fruiting, realize anti-
Season listing, to further increase its economic benefit;Any chemical agent is not used in planting process, so that collybia albuminosa is raw
It grows in organic environment, ensure that product quality, also reduce planting cost.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is annular bulkhead distributed architecture schematic diagram on porous plate;
Fig. 2 is provided with the partial structural diagram of perpendicular bulkhead;
Fig. 3 is the structural schematic diagram that plate hole submits wrong formula setting on porous plate.
1- porous plate in figure, 2- annular bulkhead, 3- plate hole, 4- annular interface, 5- erect bulkhead, 6- string hole.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical solution of the present invention will be carried out below
Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work
Other embodiment belongs to the range that the present invention is protected.
Embodiment 1
The present embodiment provides a kind of pure artificial cultivation new methods of termite series collybia albuminosa, include the following steps:
(1) culture utensil is chosen, being laid with the compost that a layer thickness is 2cm to culture utensil bottom, (compost should not press
Obtain too real and too loose), compost first carries out high-temperature sterilization, and culture soil's water content is 15.5%, spreads above the compost
If a porous plate 1, as shown in Figure 1, the outline border that the porous plate 1 is close to the culture utensil is arranged, it is same on the porous plate
Axis setting multi-turn and the plate hole 3 that is distributed in a ring, the plate hole 3 on the porous plate 1 is uniformly distributed, each on the porous plate 1
The aperture of plate hole 3 is 4mm, the distance between adjacent 3 center of plate hole be 2cm, the porous plate 1 with a thickness of 1mm;
The Nutrition Soil of corresponding each plate hole locations draws cave depth 5mm, width 4mm;It is also coaxially disposed in the top of the porous plate 1
Multiple annular bulkheads 2, the annular bulkhead 2 are located among the gap in different girth sheets holes 3;Between each adjacent two annular bulkhead 2
Two girth sheets holes 3 are set, the plate hole on different circles is arranged in a staggered manner, so that the distance between plate hole and plate hole widen, it is described
The thickness of multiple annular bulkheads 2 is 80 μm;The distance between adjacent plate hole 3 positioned at same circle is equal, positioned at same circle
The distance of multiple plate hole 3 to adjacent annular bulkheads 2 is equal;
(2) breeding termite is collected at different collybia albuminosa cavities mouths, dealation processing is carried out to breeding termite, after obtaining dealation
Breeding termite;The concrete operations that breeding termite is collected at the collybia albuminosa cavities mouth are as follows: Xiang Suoshu collybia albuminosa cavities mouth
The compressed gas that humidity is 86-88% is injected, intensity of illumination is by gradually dying down by force or gradually becoming strong by weak, and air pressure is by gradually becoming by force
Weak or strong by weak gradually change, temperature is at 26-27 DEG C;
(3) it above culture utensil, in the annular interface 4 being located between two adjacent annular bulkheads 2, shakes and uniformly throws
Breeding termite after entering dealation, the input amount of the breeding termite are twice of 3 number of plate hole in the annular interface 4, are kept
Air humidity in the culture utensil is 80%-90%, and the culture soil's water content is 15.5%-19.5%, and temperature exists
25-28 DEG C, culture breeding termite is until there is the termite to reach maturity, by the cave chamber formicary for the termite to reach maturity occur difference
It is transplanted in multiple independent culture bottles and carries out breeding culture, and be 1cm cloth in the intracavitary dispensing diameter in the cave of the cave chamber formicary
The ant nest of full collybia albuminosa mycelium pellet also adds spare dry ant nest, warp the cave of the cave chamber formicary is intracavitary in the management process
After the completion of breeding in 30 days, the ant nest bacterium nest of multiple formings is formed;
(4) compost (compost should not press too real and too loose) that a layer thickness is 1m is laid on concrete floor,
Formicary, nest the depth 45cm, diameter 30cm of the formicary are uniformly excavated on the compost;
Step (3) the ant nest bacterium nest is integrally dug out respectively later, and is implanted into the formicary respectively, with 5cm thickness
Compost cover the formicary, launched above the compost of the corresponding formicary position the edible thin sawdust of suitable termite and
Dry weeds cultivate fruiting;During management of producing mushroom, it is 13-20 DEG C that the temperature in formicary, which first controls, after being kept for 3-4 months, then is controlled
Temperature processed is 15-28 DEG C until management fruiting, keeping culture soil's water content is 15.2%-19.5%.
Embodiment 2
The present embodiment provides a kind of pure artificial cultivation new methods of termite series collybia albuminosa, include the following steps:
(1) culture utensil is chosen, is laid with the compost that a layer thickness is 20cm to culture utensil bottom, compost first carries out
High-temperature sterilization, culture soil's water content are 19.5%, one porous plate 1 of laying above the compost, on the porous plate
Coaxial arrangement multi-turn and the plate hole 3 that is distributed in a ring, the plate hole 3 on the porous plate 1 is uniformly distributed, every on the porous plate 1
The aperture of a plate hole 3 is 5mm, and the distance between 3 center of adjacent holes is 5cm, the porous plate 1 with a thickness of 20mm;
The Nutrition Soil of corresponding each plate hole locations draws cave depth 10mm, width 5mm;It is also coaxially set in the top of the porous plate 1
Multiple annular bulkheads 2 are set, the annular bulkhead 2 is located among the gap in different girth sheets holes 3;Each adjacent two annular bulkhead 2 it
Between two girth sheets holes 3 are set, the thickness of the multiple annular bulkhead 2 is 200 μm;It is arranged between two neighboring annular bulkhead 2
Several perpendicular bulkheads 5, the center of the perpendicular bulkhead 5 are respectively provided with string hole 6, and the string hole 6 on the perpendicular bulkhead 5 of difference is located at same level
On.Two plate holes 3 are arranged between two neighboring perpendicular bulkhead 5, and two plate holes 3 are located at different circles (as shown in Figure 2), the string hole
Aperture be 4-5mm so that the adjacent area that breeding ants can be separated in perpendicular bulkhead is movable back and forth;
The distance between adjacent plate hole 3 positioned at same circle is equal, positioned at same circle multiple plate holes 3 to adjacent annular every
The distance of frame 2 is equal;
(2) breeding termite is collected at different collybia albuminosa cavities mouths, dealation processing is carried out to breeding termite, after obtaining dealation
Breeding termite;The concrete operations that breeding termite is collected at the collybia albuminosa cavities mouth are as follows: Xiang Suoshu collybia albuminosa cavities mouth
The compressed gas that humidity is 88-90% is injected, intensity of illumination is by gradually dying down by force or gradually becoming strong by weak, and air pressure is by gradually becoming by force
Weak or strong by weak gradually change, temperature is at 26-28 DEG C;
(3) it above culture utensil, in the annular interface 4 being located between two adjacent annular bulkheads 2, shakes and uniformly throws
Breeding termite after entering dealation, the input amount of the breeding termite are twice of 3 number of plate hole in the annular interface 4, are kept
Air humidity in the culture utensil is 80%-90%, and the culture soil's water content is 15.5%-19.5%, and temperature exists
25-28 DEG C, culture breeding termite culture breeding termite the termite to reach maturity will occur until there is the termite to reach maturity
Cave chamber formicary, which is transplanted to respectively in multiple independent culture bottles, carries out breeding culture, and in the intracavitary dispensing in cave of the cave chamber formicary
Diameter is that 1.5cm is covered with the ant nest of collybia albuminosa mycelium pellet, in the management process, the cave of the cave chamber formicary it is intracavitary also add it is standby
Dry ant nest forms the ant nest bacterium nest of multiple formings after the completion of breeding in 45 days;
(4) it is laid with the compost that a layer thickness is 1.5m on concrete floor, uniformly excavates ant on the compost
Nest, nest the depth 55cm, diameter 50cm of the formicary;
Step (3) the ant nest bacterium nest is integrally dug out respectively later, and is implanted into the formicary respectively, with 15cm thickness
Compost cover the formicary, launched above the compost of the corresponding formicary position the edible thin sawdust of suitable termite and
Dry weeds cultivate fruiting;During management of producing mushroom, it is 13-20 DEG C that the temperature in formicary, which first controls, after being kept for 3-4 months, then is controlled
Temperature processed is 15-28 DEG C until management fruiting, keeping culture soil's water content is 15.2%-19.5%.
Embodiment 3
The present embodiment provides a kind of pure artificial cultivation new methods of termite series collybia albuminosa, include the following steps:
(1) culture utensil is chosen, is laid with the compost that a layer thickness is 10cm to culture utensil bottom, compost first carries out
High-temperature sterilization, culture soil's water content are 17.5%, one porous plate 1 of laying above the compost, on the porous plate
It is coaxially disposed multi-turn and plate hole 3 arranged in a ring, the plate hole 3 on the porous plate 1 is uniformly distributed, in the present embodiment, coaxially
The plate hole 3 of the annular array of setting and adjacent annular plate hole 3 are in alternating expression setting (as shown in Figure 3), so that adjacent plate hole 3
The distance between widen, so that the breeding ants cave chamber bred in compost be made to maintain a certain distance, prevent two or two
The phenomenon that above cave chamber interconnection merges.The aperture of each plate hole 3 is 4.5mm, adjacent panels on the porous plate 1
The distance between 3 center of hole be 3cm, the porous plate 1 with a thickness of 1.5mm;
The Nutrition Soil of corresponding each plate hole locations draws cave depth 8mm, width 4.5mm, also coaxially sets in the top of the porous plate 1
Multiple annular bulkheads 2 are set, the annular bulkhead 2 is located among the gap in different girth sheets holes 3;Each adjacent two annular bulkhead 2 it
Between a girth sheets hole 3 is set, the thickness of the multiple annular bulkhead 2 is 6 μm;Between the adjacent plate hole 3 of same circle away from
From equal, the distance positioned at multiple plate holes 3 to the adjacent annular bulkhead 2 of same circle is equal;
(2) breeding termite is collected at different collybia albuminosa cavities mouths, dealation processing is carried out to breeding termite, after obtaining dealation
Breeding termite;The concrete operations that breeding termite is collected at the collybia albuminosa cavities mouth are as follows: Xiang Suoshu collybia albuminosa cavities mouth
The compressed gas that humidity is 84-88% is injected, intensity of illumination is by gradually dying down by force or gradually becoming strong by weak, and air pressure is by gradually becoming by force
Weak or strong by weak gradually change, temperature is at 26-28 DEG C;
(3) it above culture utensil, in the annular interface 4 being located between two adjacent annular bulkheads 2, shakes and uniformly throws
Breeding termite after entering dealation, the input amount of the breeding termite are twice of the 4 upper plate number of perforations of annular interface, keep institute
Stating the air humidity in culture utensil is 80%-90%, and the culture soil's water content is 15.5%-19.5%, and temperature is in 25-
28 DEG C, culture breeding termite moves the cave chamber formicary for the termite to reach maturity occur up to there is the termite to reach maturity respectively
Progress breeding culture in multiple independent culture bottles is planted, and is 1.2cm cloth in the intracavitary dispensing diameter in the cave of the cave chamber formicary
The ant nest of full collybia albuminosa mycelium pellet also adds spare dry ant nest, warp the cave of the cave chamber formicary is intracavitary in the management process
After the completion of breeding in 40 days, the ant nest bacterium nest of multiple formings is formed;
(4) it is laid with the compost that a layer thickness is 1.2m on concrete floor, uniformly excavates ant on the compost
Nest, nest the depth 50cm, diameter 40cm of the formicary;
Step (3) the ant nest bacterium nest is integrally dug out respectively later, and is implanted into the formicary respectively, with 10cm thickness
Compost cover the formicary, launched above the compost of the corresponding formicary position the edible thin sawdust of suitable termite and
Dry weeds cultivate fruiting;During management of producing mushroom, it is 13-20 DEG C that the temperature in formicary, which first controls, after being kept for 3-4 months, then is controlled
Temperature processed is 15-28 DEG C until management fruiting, keeping culture soil's water content is 15.2%-19.5%.
Embodiment 4
The present embodiment provides a kind of pure artificial cultivation new methods of termite series collybia albuminosa, include the following steps:
(1) culture utensil is chosen, is laid with the compost that a layer thickness is 10cm to culture utensil bottom, compost first carries out
High-temperature sterilization, culture soil's water content are 17.5%, one porous plate 1 of laying above the compost, on the porous plate
Coaxial arrangement multi-turn and the plate hole 3 that is distributed in a ring, the plate hole 3 on the porous plate 1 is uniformly distributed, every on the porous plate 1
The aperture of a plate hole 3 is 4.5mm, the distance between adjacent 3 center of plate hole be 4cm, the porous plate 1 with a thickness of 1.5mm;
The Nutrition Soil of corresponding each plate hole locations draws cave depth 6mm, width 4.5mm, also coaxially sets in the top of the porous plate 1
Multiple annular bulkheads 2 are set, the annular bulkhead 2 is located among the gap in different girth sheets holes 3;Each adjacent two annular bulkhead 2 it
Between a girth sheets hole 3 is set, the thickness of the multiple annular bulkhead 2 is 1mm;Between the adjacent plate hole 3 of same circle away from
From equal, the distance positioned at multiple plate holes 3 to the adjacent annular bulkhead 2 of same circle is equal;
(2) breeding termite is collected at different collybia albuminosa cavities mouths, dealation processing is carried out to breeding termite, after obtaining dealation
Breeding termite;The concrete operations that breeding termite is collected at the collybia albuminosa cavities mouth are as follows: Xiang Suoshu collybia albuminosa cavities mouth
The compressed gas that humidity is 80-85% is injected, intensity of illumination is by gradually dying down by force or gradually becoming strong by weak, and air pressure is by gradually becoming by force
Weak or strong by weak gradually change, temperature is at 25-26 DEG C;
(3) it above culture utensil, in the annular interface 4 being located between two adjacent annular bulkheads 2, shakes and uniformly throws
Breeding termite after entering dealation, the input amount of the breeding termite are twice of the 4 upper plate number of perforations of annular interface, keep institute
Stating the air humidity in culture utensil is 80%-90%, and the culture soil's water content is 15.5%-19.5%, and temperature is in 25-
28 DEG C, culture breeding termite moves the cave chamber formicary for the termite to reach maturity occur up to there is the termite to reach maturity respectively
Progress breeding culture in multiple independent culture bottles is planted, and is 1.2cm cloth in the intracavitary dispensing diameter in the cave of the cave chamber formicary
The ant nest of full collybia albuminosa mycelium pellet also adds spare dry ant nest, warp the cave of the cave chamber formicary is intracavitary in the management process
After the completion of breeding in 40 days, the ant nest bacterium nest of multiple formings is formed;
(4) it is laid with the compost that a layer thickness is 1.2m on concrete floor, uniformly excavates ant on the compost
Nest, nest the depth 50cm, diameter 40cm of the formicary;
Step (3) the ant nest bacterium nest is integrally dug out respectively later, and is implanted into the formicary respectively, with 10cm thickness
Compost cover the formicary, launched above the compost of the corresponding formicary position the edible thin sawdust of suitable termite and
Dry weeds cultivate fruiting;During management of producing mushroom, it is 13-20 DEG C that the temperature in formicary, which first controls, after being kept for 3-4 months, then is controlled
Temperature processed is 15-28 DEG C until management fruiting, keeping culture soil's water content is 15.2%-19.5%.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of pure artificial cultivation new method of termite series collybia albuminosa, which comprises the steps of:
(1) culture utensil to be chosen, is laid with one layer of compost to culture utensil bottom, compost moisture content is 15.5%-19.5%,
A porous plate is laid with above the compost, the plate hole for being coaxially disposed multi-turn on the porous plate and being distributed in a ring is right
The Nutrition Soil of each plate hole locations is answered to draw cave depth 5-10mm, diameter 4-5mm;It is also coaxially disposed in the top of the porous plate multiple
Annular bulkhead, the annular bulkhead are located among the gap in different girth sheets holes;Positioned at the distance between the adjacent plate hole of same circle
Equal, the distance positioned at multiple plate holes to the adjacent annular bulkhead of same circle is equal;
(2) breeding termite is collected at different collybia albuminosa cavities mouths, and dealation processing is carried out to breeding termite, it is numerous after obtaining dealation
Grow termite;
(3) above culture utensil, in the annular interface being located between two adjacent annular bulkheads, uniformly investment dealation is shaken
Breeding termite afterwards, the input amount of the breeding termite are twice of the annular interface upper plate number of perforations, keep the culture
Air humidity in utensil is 80%-90%, and the culture soil's water content is 15.5%-19.5%, temperature at 25-28 DEG C,
The cave chamber formicary for the termite to reach maturity occur is transplanted to more by culture breeding termite respectively up to there is the termite to reach maturity
Breeding culture is carried out in a separate device, and is covered with the ant nest of collybia albuminosa mycelium pellet in the intracavitary dispensing in the cave of the cave chamber formicary,
In the management process, spare dry ant nest is also added the cave of the cave chamber formicary is intracavitary, after the completion of breeding, form multiple formings
Ant nest bacterium nest;
(4) it is laid with one layer of compost on concrete floor, formicary is uniformly excavated on the compost, later by step (3)
The ant nest bacterium nest is integrally dug out respectively, and is implanted into the formicary respectively, covers the ant with the compost of 5-15cm thickness
Nest cultivates fruiting;During management of producing mushroom, it is 13-20 DEG C that the temperature in formicary, which first controls, after being kept for 3-4 months, then controls temperature
Degree is for 15-28 DEG C until management fruiting, keeping culture soil's water content is 15.2%-19.5%.
2. the pure artificial cultivation new method of termite series collybia albuminosa according to claim 1, which is characterized in that step (1)
In, culture utensil bottom be laid with compost with a thickness of 2-20cm.
3. the pure artificial cultivation new method of termite series collybia albuminosa according to claim 1, which is characterized in that step (1)
In, the plate hole on the porous plate is uniformly distributed, and the aperture of each plate hole is 4-5mm on the porous plate, in adjacent plate hole
The distance between heart be 2-5cm, the porous plate with a thickness of 1-20mm;
Several perpendicular bulkheads are set between two neighboring annular bulkhead, and the center of each perpendicular bulkhead is respectively provided with string hole, adjacent
Two plate holes are arranged between two perpendicular bulkheads, and two plate holes are located at different circles.
4. the pure artificial cultivation new method of termite series collybia albuminosa according to claim 1, which is characterized in that step (1)
In, the compost first carries out high-temperature sterilization;
Two girth sheets holes are set between each adjacent two annular bulkhead, and the thickness of the multiple annular bulkhead is 0.006-1mm.
5. the pure artificial cultivation new method of termite series collybia albuminosa according to claim 1, which is characterized in that step (2)
In, the concrete operations that breeding termite is collected at the collybia albuminosa cavities mouth are as follows: Xiang Suoshu collybia albuminosa cavities mouth injects humidity
For the compressed gas of 80-90%, intensity of illumination is by gradually dying down by force or gradually becoming strong by weak, and air pressure is by gradually dying down by force or by weak
Gradually become strong, temperature is at 25-28 DEG C.
6. the pure artificial cultivation new method of termite series collybia albuminosa according to claim 1, which is characterized in that step (3)
In, the separate device for carrying out the breeding culture is culture bottle;
The diameter of the ant nest for being covered with collybia albuminosa mycelium pellet is 0.5-1cm.
7. the pure artificial cultivation new method of termite series collybia albuminosa according to claim 1, which is characterized in that step (3)
In, the time of the breeding is 30-45 days.
8. the pure artificial cultivation new method of termite series collybia albuminosa according to claim 1, which is characterized in that step (4)
In, the compost with a thickness of 1-1.5m.
9. the pure artificial cultivation new method of termite series collybia albuminosa according to claim 1, which is characterized in that step (4)
In, nest the depth 45-55cm, diameter 30-50cm of the formicary.
10. the pure artificial cultivation new method of termite series collybia albuminosa according to claim 1, which is characterized in that step (4)
In, it cultivates in the management process of fruiting, also to the thin sawdust of dispensing and dry weeds above the compost of the correspondence formicary position.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110810132A (en) * | 2019-12-05 | 2020-02-21 | 吉林农业大学 | Fungus cultivation device and application thereof in black fungus planting |
CN112715274A (en) * | 2020-12-28 | 2021-04-30 | 昆明绿耳生物科技有限责任公司 | Termite-termitomyces albuminosus symbiotic seedling and preparation method thereof |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102783363A (en) * | 2012-08-30 | 2012-11-21 | 张学西 | Artificial breeding method of termite mushroom |
CN206100991U (en) * | 2016-08-30 | 2017-04-19 | 云南秋成农业科技有限公司 | Collybia albuminosa artificial propagation device |
CN206100993U (en) * | 2016-08-30 | 2017-04-19 | 云南秋成农业科技有限公司 | A collybia albuminosa artificial breeding section of thick bamboo |
CN107493972A (en) * | 2017-09-26 | 2017-12-22 | 张学西 | A kind of pure artificial cultivation method of termite series collybia albuminosa |
-
2019
- 2019-01-10 CN CN201910022658.5A patent/CN109548564A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102783363A (en) * | 2012-08-30 | 2012-11-21 | 张学西 | Artificial breeding method of termite mushroom |
CN206100991U (en) * | 2016-08-30 | 2017-04-19 | 云南秋成农业科技有限公司 | Collybia albuminosa artificial propagation device |
CN206100993U (en) * | 2016-08-30 | 2017-04-19 | 云南秋成农业科技有限公司 | A collybia albuminosa artificial breeding section of thick bamboo |
CN107493972A (en) * | 2017-09-26 | 2017-12-22 | 张学西 | A kind of pure artificial cultivation method of termite series collybia albuminosa |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110810132A (en) * | 2019-12-05 | 2020-02-21 | 吉林农业大学 | Fungus cultivation device and application thereof in black fungus planting |
CN110810132B (en) * | 2019-12-05 | 2021-08-03 | 吉林农业大学 | Fungus cultivation device and application thereof in black fungus planting |
CN112715274A (en) * | 2020-12-28 | 2021-04-30 | 昆明绿耳生物科技有限责任公司 | Termite-termitomyces albuminosus symbiotic seedling and preparation method thereof |
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