CN101731183A - Odontothrips loti feeding method - Google Patents
Odontothrips loti feeding method Download PDFInfo
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- CN101731183A CN101731183A CN200810180442A CN200810180442A CN101731183A CN 101731183 A CN101731183 A CN 101731183A CN 200810180442 A CN200810180442 A CN 200810180442A CN 200810180442 A CN200810180442 A CN 200810180442A CN 101731183 A CN101731183 A CN 101731183A
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Abstract
The invention provides an odontothrips loti feeding method, relating to the insect feeding field. The existing odontothrips loti feeding method has the disadvantages that odontothrips loti is low in survival rate, poor in vigour, small in quantity and serious in generation overlapping, adults with high vigour in the same instar are difficult to obtain, and technological operation is complex. In the invention, odontothrips loti female is directly placed on blade to lay eggs, moist water sucking material is used for enveloping petiole and dropping water to the water sucking material at regular intervals, so as to keep the blade to be alive, the blade is placed into a climatic chamber, massive nymphs are obtained through hatching, and then massive adults are obtained through feeding on nymph and pupa. The method of the invention has high hatching rate and survival rate and can obtain massive odontothrips lotis of all kinds of stages which are uniform in polypide and high in vigour. The method is simple and convenient in operation, only requiring keeping the medicago blade to be alive by dropping water and regulating the humidity of cotton layer at the bottom of inset feeding bottle. The invention also has the advantages of available material, low apparatus construction cost and saving cost.
Description
Technical field
The present invention relates to the raising method of a kind of insect, relate in particular to the raising method of a kind of Birdfoot tooth thrips.
Background technology
The thrips class pest of whole world harm clover has kind more than 20, and from different places, the kind difference.In China northern clover producing region, based on Birdfoot tooth thrips, China's alfalfa variety is reached more than 90% by the generally rate that thrips endangers at present.Birdfoot tooth thrips polypide is small, just incubates the nymph body and is about and is 0.2-0.4mm, and wide about 0.1mm becomes polypide to be about and is 1-2mm, the wide about 0.4mm of body; It grows very fast, and it is all very short that each worm attitude is gone through the phase, and generation overlap is serious, and fertility is strong, and a breeding generation only needs about 25 days.The suitableeest mean temperature that Birdfoot tooth thrips grows breeding is 20-25 ℃, and relative moisture is 60-70%.Food alfalfa plants tender tissue is got in Birdfoot tooth thrips happiness, often ovum is produced in the young leaves that does not launch, and treats that ovum hatching teleonymph just gets food in young leaves, and the lighter causes the upper blade distortion, and weight person clover blade and bud dry up, caducous.Seedling stage and branch phase harm lobus cardiacus and tender leaf; Bloom and productive phase the main harm floral organ, in spending, smash pollen to pieces, destroy column cap.The lobus cardiacus of thrips harm and tender leaf are along with leaf growth, both sides begin to curl gradually, shrinkage, flavescence, and killed plant is short and small, undergrowth, thrips harm can cause the alfalfa hay production loss more than 14.9%, it is about 32.84% that crude protein content decline 8.76%-11.56%, crude fat descend, and carotin descends 18.74%, calcium reduces 53.85%-70%, phosphorus reduces 15.79%, and the plant total amino acid content of being injured descends 36.35%, has increased by 12.18% and raw fiber is the highest.This shows that thrips has a strong impact on the output and the quality of clover grass, is present China clover, especially the insect of the numerous kind of tool danger of clover.How to prevent and treat the thrips insect pest effectively, become one of important topic of China's alfalfa industry development.
Because Birdfoot tooth thrips has that development duration is short, fertility is strong, generation overlap is serious, individuality is little, easily hidden, to characteristics such as insecticide very easily develop immunity to drugs, so be difficult to obtain ideal control effect with single prophylactico-therapeutic measures.Utilizing the kind resistance to insects to control its harm, is to explore one of important channel that fundamentally solves thrips harm.But, can not satisfy a large amount of worms source of research needs, thereby limit the further investigation of the anti-thrips of clover because the breeding technology of Birdfoot tooth thrips is still unresolved.
At present, obtain the worm source and mainly take the method for nature collection and artificial feeding, naturally gather and promptly directly obtain a large amount of Birdfoot tooth thrips adult, nymph in the clover land for growing field crops, but this class insect portability and transmitted virus disease, and then influence result of study, extremely small, fragile because of the thrips nymph in addition, and generation overlap is serious, and a large amount of worm unanimities in age of very difficult acquisition, the polypide that vigor is high are studied.Rearing method comprises concave slide method for breeding, penicillin bottle method for breeding, potted plant clover method for breeding.The concave slide method for breeding is to be involutory together with two concave slides, places no worm clover fresh leaf fritter in every pair of concave slide, places the ovum of thrips on the leaf piece, concave slide is fixed with rubber band, be placed in the porcelain basin, the porcelain basin put into climatic cabinate raise, changed blade every 2～3 days.Be that concave surface with two concave slides is involutory and forms the environment of a sealing in the concave slide method for breeding, provide the environment of a safety sealing to raise alfalfa thrips, and prevent that alfalfa thrips from escaping, this is its advantage.But the activity space in the concave slide is little, and blade is breathed the high humidity that causes, and the thrips vigor is low, survival rate is low.Thrips escapes easily when changing blade in addition, especially one age nymph, movable fast, faint, be difficult for during escape catching the method operating difficulties.
The penicillin bottle method for breeding is a place mat thin layer absorbent cotton in the penicillin bottle, covers the filter paper with end diameter on absorbent cotton, adds suitable quantity of water, seals bottleneck, prevents the thrips escape with 100 orders 5cm * 5cm gauze net cover rubber band.Put into one in thrips ovum and fresh clover blade in every bottle, on filter paper at the bottom of the bottle, add suitable quantity of water, keep humidity in the bottle with the 2ml syringe.Seal bottleneck with the gauze net cover rubber band, put into the porcelain basin, in 25 ℃ of climatic cabinates, raise, changed blade every 2-3 days.The advantage of penicillin bottle method for breeding is its material cheapness that is easy to get, the survival rate height of thrips, and shortcoming is operation inconvenience, especially changes blade, seal the envelope of getting of gauze, and every bottle can only be raised a small amount of insect population.
Potted plant clover method for breeding is a potted plant clover under no worm environment, connects worm in the branch phase and raises.This method can obtain the high Birdfoot tooth thrips of a large amount of vigor near natural conditions, but because Birdfoot tooth thrips belongs to the microminiature insect, catch difficulty, and generation overlap is serious, is difficult to obtain the polypide of worm unanimity in age.
Concave slide method for breeding, penicillin bottle method for breeding, potted plant clover method for breeding are mainly used in the research of the biological property aspect of Birdfoot tooth thrips, few for need worm amount, research as about 100, these methods are feasible, but all there is operation inconvenience, the lethality height of Birdfoot tooth thrips is difficult to obtain insect population in enormous quantities (about 1000 s') shortcoming.
Therefore, solving the problem that prior art exists, realize the mass rearing of thrips, obtain a large amount of worm unanimities in age, do not carry viral Birdfoot tooth thrips worm source, is the prerequisite of launching the research of kind resistance to insects.
The creativity and innovation content
The raising method that the purpose of this invention is to provide a kind of Birdfoot tooth thrips, operation inconvenience, the number of animals raised that the existing raising method of solution exists is few, the thrips survival rate is low, the problem of vigor difference, and adopts and support worm ware, foster worm bottle for raising instrument, and be with low cost.
For achieving the above object, method of the present invention in turn includes the following steps:
(1) acquisition of ovum and hatching
1. in foster worm ware, place the multi-disc cloverleaf, the female worm of Birdfoot tooth thrips is placed on the blade lay eggs;
2. rest the worm ware then and place climate box;
3. remove female worm after 1 day, treat the ovum hatching;
4. the ovum hatching obtains nymph after 4-5 days;
(2) raising of nymph and pupa
1. the nymph with step (1) gained moves in the foster worm bottle;
2. rest the worm bottle then and place climate box, and in time put into young leaves according to the blade freshness;
3. 5-7 days teleonymphs begin to pupate, and keep ambient humidity this moment;
4. pupa is sprouted wings into adult after 4-5 days;
(3) raising of adult
1. the adult of step (2) gained is shifted out former foster worm bottle and be placed on the blade, blade is moved into new supporting in the worm bottle;
2. will newly support the worm bottle then and place climate box, and in time put into young leaves according to the blade freshness.
For keeping the blade vigor, wrap petiole in this raising method with moistening absorbent material, moistening in the feeding process to the absorbent material maintenance of dripping.
For preventing the thrips escape, add a cover preservative film at foster worm ware mouth, foster worm bottleneck, on preservative film, prick a plurality of apertures.
For keeping supporting humidity in the worm bottle, shop one deck hygroscopic material covers filter paper on hygroscopic material at the bottom of foster worm bottle, splashes into sterile water, keeps hygroscopic material moistening.
The condition that climate box is set is temperature 23.5-25.5 ℃, and relative moisture is 65-70%, photoperiod L: D=14: 10h.
This raising method is mainly used in rear cattle corner piece tooth thrips in batches.
The present invention has following beneficial effect:
1, the incubation rate of thrips ovum of the present invention is more than 95%, the raising survival rate of thrips nymph is more than 90%, the survival rate of pupa is more than 80%, eclosion rate is more than 60%, each foster worm bottle can obtain the Birdfoot tooth thrips adult more than 80, and each worm attitude polypide of the thrips of acquisition is neat, vigor is high.
2, this raising method is easy and simple to handle, and the moisture absorption layer humidity that vigor that only need keep the clover blade by dripping in the feeding process and adjusting are supported at the bottom of the worm bottle bottle gets final product.
3, this raising method uses and supports the worm ware, foster worm bottle is made the raising instrument, has advantage with low cost.
Specific embodiment
The following examples are used for explaining in more detail the present invention, but do not limit the present invention.
Embodiment 1
(1) acquisition of ovum and hatching
1. placing the multi-disc cloverleaf in the foster worm ware of diameter 90mm, is that the cotton balls of 10mm wraps petiole with diameter, the female worm of Birdfoot tooth thrips is placed on the blade by 2/sheet lay eggs, and adds a cover preservative film in foster worm ware, with No. 1 insect pinprick a plurality of apertures is arranged on the preservative film;
2. rest the worm ware then and place climate box, the climate box condition setting is 25.5 ℃, relative moisture 65%, and the photoperiod is 14: 10h, keep moistening with dropper to the cotton balls 0.2ml that drips in the feeding process, cotton balls fully contacts all the time with petiole;
3. remove female worm after 1 day, treat the ovum hatching;
4. the ovum hatching obtains nymph after 4-5 days;
(2) raising of nymph and pupa
1. at the bottom of being the foster worm bottle of 10cm, diameter spreads the absorbent cotton that a layer thickness is 2mm, on absorbent cotton, cover filter paper, the sterile water that splashes into 1ml is moistening, the nymph of step (1) gained is connected in blade, the foster worm bottle of cotton balls immigration, 10 every bottle, support the worm bottle and seal with preservative film, preservative film has a plurality of apertures with No. 1 insect pinprick.
2. rest the worm bottle then and place climate box, the climate box condition is identical with step (1), keep moistening to each cotton balls 0.2ml that drips every day in the feeding process, and according to the blade freshness young leaves is put into and to be supported the worm bottle, treats that nymph is transferred to automatically to remove old leaf on the young leaves again.
3. 5-7 days teleonymphs begin to pupate, and reduce cotton balls this moment drips to each cotton balls 0.1ml every day, in time changes blade according to the blade freshness;
4. pupa is sprouted wings into adult after 4-5 days;
(3) raising of adult
1. place blade new supporting in the worm bottle, the adult of step (2) gained is shifted out former foster worm bottle be placed on the blade by 2/sheet, blade is moved into new supporting in the worm bottle, it is identical with former foster worm bottle specification newly to support the worm bottle, add a cover preservative film at foster worm bottleneck, on preservative film with No. 1 a plurality of apertures of insect pinprick;
2. will newly support the worm bottle then and place climate box, it is identical with step (1) that climate box is provided with condition, and in time put into young leaves according to the blade freshness.
Embodiment 2
(1) acquisition of ovum and hatching
1. in the foster worm ware of diameter 150mm, place the multi-disc cloverleaf, wrap petiole, the female worm of Birdfoot tooth thrips is placed on the blade by 3/sheet lay eggs, in foster worm ware, add a cover preservative film, with No. 1 insect pinprick a plurality of apertures are arranged on the preservative film with moistening sponge;
2. rest the worm ware then and place climate box, the climate box condition setting is 23.5 ℃, relative moisture 70%, and the photoperiod is 14: 10h, dripping to sponge with dropper in the feeding process keeps moistening, and sponge fully contacts all the time with petiole;
3. remove female worm after 1 day, treat the ovum hatching;
4. the ovum hatching obtains nymph after 4-5 days;
(2) raising of nymph and pupa
1. at the bottom of being the foster worm bottle of 10cm, diameter spreads the foamed plastics that a layer thickness is 3mm, on foamed plastics, cover filter paper, the sterile water that splashes into 1ml is moistening, the nymph of step (1) gained is connected in blade, the foster worm bottle of cotton balls immigration, 10 every bottle, in foster worm ware, add a cover preservative film, with No. 1 insect pinprick a plurality of apertures are arranged on the preservative film.
2. rest the worm bottle then and place climate box, the climate box condition is identical with step (1), the 0.2ml that drips on every part of sponge keeps moistening every day in the feeding process, and according to the blade freshness young leaves is put into and to be supported the worm bottle, treats that nymph is transferred to automatically to remove old leaf on the young leaves again.
3. 5-7 days teleonymphs begin to pupate, and reduce sponge this moment drips to each cotton balls 0.1ml every day, in time changes blade according to the blade freshness;
4. pupa is sprouted wings into adult after 4-5 days;
(3) raising of adult
1. place blade new supporting in the worm bottle, the adult of step (2) gained is shifted out former foster worm bottle be placed on the blade by 2-3 head/sheet, blade is moved into new supporting in the worm bottle, it is identical with former foster worm bottle specification newly to support the worm bottle, add a cover preservative film at foster worm bottleneck, on preservative film with No. 1 a plurality of apertures of insect pinprick;
2. will newly support the worm bottle then and place climate box, it is identical with step (1) that climate box is provided with condition, and in time put into young leaves according to the blade freshness.
Embodiment 3
(1) acquisition of ovum and hatching
1. placing the multi-disc cloverleaf in the foster worm ware of diameter 90mm, is that the cotton balls of 10mm wraps petiole with diameter, the female worm of Birdfoot tooth thrips is placed on the blade by 3/sheet lay eggs, and adds a cover preservative film in foster worm ware, with No. 1 insect pinprick a plurality of apertures is arranged on the preservative film;
2. rest the worm ware then and place climate box, the climate box condition setting is 24.5 ℃, relative moisture 70%, and the photoperiod is 14: 10h, keep moistening with dropper to the cotton balls 0.2ml that drips in the feeding process, cotton balls fully contacts all the time with petiole;
3. remove female worm after 1 day, treat the ovum hatching;
4. the ovum hatching obtains nymph after 4-5 days;
(2) raising of nymph and pupa
1. at the bottom of being the foster worm bottle of 10cm, diameter spreads the absorbent cotton that a layer thickness is 3mm, on absorbent cotton, cover filter paper, the sterile water that splashes into 1ml is moistening, the nymph of step (1) gained is connected in blade, the foster worm bottle of cotton balls immigration, 10 every bottle, support the worm bottle and seal with preservative film, preservative film has a plurality of apertures with No. 1 insect pinprick.
2. rest the worm bottle then and place climate box, the climate box condition is identical with step (1), keep moistening to each cotton balls 0.2ml that drips every day in the feeding process, and according to the blade freshness young leaves is put into and to be supported the worm bottle, treats that nymph is transferred to automatically to remove old leaf on the young leaves again.
3. 5-7 days teleonymphs begin to pupate, and reduce cotton balls this moment drips to each cotton balls 0.1ml every day, in time changes blade according to the blade freshness;
4. pupa is sprouted wings into adult after 4-5 days;
(3) raising of adult
1. place blade new supporting in the worm bottle, the adult of step (2) gained is shifted out former foster worm bottle be placed on the blade by 2-3 head/sheet, blade is moved into new supporting in the worm bottle, it is identical with former foster worm bottle specification newly to support the worm bottle, add a cover preservative film at foster worm bottleneck, on preservative film with No. 1 a plurality of apertures of insect pinprick;
2. will newly support the worm bottle then and place climate box, it is identical with step (1) that climate box is provided with condition, and in time put into young leaves according to the blade freshness.
Claims (7)
1. the raising method of a Birdfoot tooth thrips is characterized in that comprising the steps:
(1) acquisition of ovum and hatching
1. in foster worm ware, place the multi-disc cloverleaf, the female worm of Birdfoot tooth thrips is placed on the blade lay eggs;
2. rest the worm ware then and place climate box;
3. remove female worm after 1 day, treat the ovum hatching;
4. the ovum hatching obtains nymph after 4-5 days;
(2) raising of nymph and pupa
1. the nymph with step (1) gained moves in the foster worm bottle;
2. rest the worm bottle then and place climate box, and in time put into young leaves according to the blade freshness;
3. 5-7 days teleonymphs begin to pupate, and keep ambient humidity this moment;
4. pupa is sprouted wings into adult after 4-5 days;
(3) raising of adult
1. the adult of step (2) gained is shifted out former foster worm bottle and be placed on the blade, blade is moved into new supporting in the worm bottle;
2. will newly support the worm bottle then and place climate box, and in time put into young leaves according to the blade freshness.
2. the raising method of a kind of Birdfoot tooth thrips according to claim 1 is characterized in that wrapping petiole with moistening absorbent material, and dripping to absorbent material in the feeding process keeps moistening.
3. the raising method of a kind of Birdfoot tooth thrips according to claim 1 and 2, its feature is added a cover preservative film at foster worm ware mouth, foster worm bottleneck, pricks a plurality of apertures on preservative film.
4. according to the raising method of claim 1 or 2 described a kind of Birdfoot tooth thrips, spread one deck absorbent material at the bottom of it is characterized in that supporting the worm bottle in the step (2), on absorbent material, cover filter paper, splash into sterile water, keep absorbent material moistening.
5. the raising method of a kind of Birdfoot tooth thrips according to claim 3 is spread one deck absorbent material at the bottom of it is characterized in that supporting the worm bottle in the step (2), covers filter paper on absorbent material, splashes into sterile water, keeps absorbent material moistening.
6. the raising method of a kind of Birdfoot tooth thrips according to claim 5 is characterized in that absorbent material is absorbent cotton or foamed plastics or sponge.
7. the raising method of a kind of Birdfoot tooth thrips according to claim 1, the acquisition and the raising stage of hatching stage or nymph and pupa or the raising stage of adult that it is characterized in that ovum, it is temperature 23.5-25.5 ℃ that climate box is provided with condition, relative moisture is 65-70%, photoperiod L: D=14: 10h.
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| CN200810180442A CN101731183A (en) | 2008-11-27 | 2008-11-27 | Odontothrips loti feeding method |
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| CN102524188A (en) * | 2012-01-20 | 2012-07-04 | 福建省农业科学院植物保护研究所 | Insect breeding bottom and breeding method of gynaikothrips ficorum |
| CN102550493A (en) * | 2012-01-17 | 2012-07-11 | 甘肃农业大学 | Method for artificial infestation of Odontothrips loti on alfalfa |
| CN103004698A (en) * | 2012-11-27 | 2013-04-03 | 天津滨海国际花卉科技园区股份有限公司 | Method for measuring influence of humidity on scirtothrips dorsalis population |
| CN103053477A (en) * | 2012-12-26 | 2013-04-24 | 中国农业科学院郑州果树研究所 | Scale breeding method of Scolothrips takahashii |
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| CN104872068A (en) * | 2015-05-26 | 2015-09-02 | 中国热带农业科学院环境与植物保护研究所 | Indoor insectary and culture method for thrips hawaiiensis |
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| CN102550493A (en) * | 2012-01-17 | 2012-07-11 | 甘肃农业大学 | Method for artificial infestation of Odontothrips loti on alfalfa |
| CN102524188A (en) * | 2012-01-20 | 2012-07-04 | 福建省农业科学院植物保护研究所 | Insect breeding bottom and breeding method of gynaikothrips ficorum |
| CN103004698A (en) * | 2012-11-27 | 2013-04-03 | 天津滨海国际花卉科技园区股份有限公司 | Method for measuring influence of humidity on scirtothrips dorsalis population |
| CN103053477A (en) * | 2012-12-26 | 2013-04-24 | 中国农业科学院郑州果树研究所 | Scale breeding method of Scolothrips takahashii |
| CN103053477B (en) * | 2012-12-26 | 2014-01-22 | 中国农业科学院郑州果树研究所 | Scale breeding method of Scolothrips takahashii |
| CN103355257A (en) * | 2013-07-15 | 2013-10-23 | 云南大学 | Breeding method and breeding box for insect larvae |
| CN104012472B (en) * | 2014-03-12 | 2016-08-17 | 云南农业大学 | The indoor feeding method of thrips |
| CN104012472A (en) * | 2014-03-12 | 2014-09-03 | 云南农业大学 | Indoor breeding method of thrips |
| CN103975903B (en) * | 2014-06-03 | 2015-12-30 | 周利平 | The raising method of a kind of butterfly section hairstreak |
| CN103975903A (en) * | 2014-06-03 | 2014-08-13 | 周利平 | Method for breeding lycaenidae butterflies |
| CN104782575A (en) * | 2014-12-09 | 2015-07-22 | 青岛农业大学 | Method for collecting and feeding tobacco thrips |
| CN104872068A (en) * | 2015-05-26 | 2015-09-02 | 中国热带农业科学院环境与植物保护研究所 | Indoor insectary and culture method for thrips hawaiiensis |
| CN104872068B (en) * | 2015-05-26 | 2018-11-16 | 中国热带农业科学院环境与植物保护研究所 | Worm device and method for breeding are supported in a kind of Huang chest thrips room |
| CN105393993A (en) * | 2015-12-28 | 2016-03-16 | 北京市农林科学院 | Method for artificial mass-reproduction of Echinothrips americanus Morgan |
| CN110432231A (en) * | 2019-09-05 | 2019-11-12 | 中国农业科学院植物保护研究所 | The method of single head raising South America tomato leaf miner |
| CN110432231B (en) * | 2019-09-05 | 2021-05-25 | 中国农业科学院植物保护研究所 | Method for breeding Nanmei tomato leaf miner with single head |
| CN111466344A (en) * | 2020-04-23 | 2020-07-31 | 广西大学 | Method for continuously feeding thrips |
| CN113273547A (en) * | 2021-05-24 | 2021-08-20 | 吉林农业大学 | Breeding device and breeding method for cynara scolymus |
| CN118140877A (en) * | 2024-05-11 | 2024-06-07 | 云南千淇生物科技有限公司 | Breeding device and breeding method for breeding Lepidius crassipes |
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Application publication date: 20100616 |