CN111466344A - Method for continuously feeding thrips - Google Patents
Method for continuously feeding thrips Download PDFInfo
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- CN111466344A CN111466344A CN202010326526.4A CN202010326526A CN111466344A CN 111466344 A CN111466344 A CN 111466344A CN 202010326526 A CN202010326526 A CN 202010326526A CN 111466344 A CN111466344 A CN 111466344A
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- thrips
- ima
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- cage
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- 241001414989 Thysanoptera Species 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 24
- 241000238631 Hexapoda Species 0.000 claims abstract description 45
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 40
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 40
- 230000032669 eclosion Effects 0.000 claims abstract description 27
- 230000012447 hatching Effects 0.000 claims abstract description 21
- 230000019617 pupation Effects 0.000 claims abstract description 20
- 239000002689 soil Substances 0.000 claims description 14
- 235000013601 eggs Nutrition 0.000 claims description 13
- 238000009395 breeding Methods 0.000 claims description 9
- 230000001488 breeding effect Effects 0.000 claims description 9
- 230000017448 oviposition Effects 0.000 claims description 9
- 238000007789 sealing Methods 0.000 claims description 9
- 238000002474 experimental method Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 239000012780 transparent material Substances 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 3
- 235000013305 food Nutrition 0.000 claims description 3
- 230000000630 rising effect Effects 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 2
- 241000927584 Frankliniella occidentalis Species 0.000 description 22
- 230000009286 beneficial effect Effects 0.000 description 4
- 238000004166 bioassay Methods 0.000 description 4
- 230000006378 damage Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000189579 Thripidae Species 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 208000032544 Cicatrix Diseases 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 241000016010 Tomato spotted wilt orthotospovirus Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000037387 scars Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention belongs to the technical field of insect feeding, and particularly relates to a method for continuously feeding thrips, which comprises the following steps of (1) L ima bean cultivation, (2) thrips feeding, (3) thrips pupation and hatching, (4) thrips eclosion.
Description
Technical Field
The invention belongs to the technical field of insect feeding, and particularly relates to a method for continuously feeding thrips.
Background
Frankliniella occidentalis, also known as alfalfa thrips, belongs to the order Thysanoptera, thrips family Thripidae. The insect was native to north america, was first discovered in 1955 in hawaii, and was once the most common thrips in california in the united states. Since the 80 s of the 20 th century, it became a strong species with increased resistance to various environments and pesticides, and thus gradually expanded outward.
The frankliniella occidentalis has great harm to crops. The insect feeds on the stem, leaf, flower and fruit of the plant by a rasping type oral organ, so that the petal is discolored, the leaf is shriveled, the stem and the fruit form scars, and finally the plant is withered, and various viruses including tomato spotted wilt virus are spread. It is known that this insect has caused 50-90% reduction in tomato production in Hawaii, USA.
In order to research the prevention and control method of Frankliniella occidentalis, a large amount of Frankliniella occidentalis larvae are generally required to be used in laboratory bioassay, so that the research of a method for continuously feeding Frankliniella occidentalis is particularly important.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the method for continuously feeding the thrips, which is beneficial to feeding a large number of regular and consistent experimental insects and plays an important role in indoor bioassay experiments.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for continuously feeding thrips comprises the following steps:
(1) l cultivation of ima bean, selecting L ima bean with plump seeds, no insect population and no mildew, planting in a greenhouse flowerpot, watering and fertilizing every day until L ima bean grows to blossom and pod for use;
(2) breeding thrips, namely picking up L ima bean pods 10-15, taking the bean pods back to a laboratory, placing the bean pods in an insect cage, placing the bean pods in an egg-laying pupation hatching device with the bottom of 5cm in diameter, the mouth of 8cm in diameter and the height of 8cm, releasing 100 heads of adult thrips into the insect cage, and allowing the thrips to take food and lay eggs on L ima beans;
(3) after the thrips lay eggs for 2 days, catching up the thrips imagoes on L ima beans, taking out L ima beans and a spawning pupation hatching device, independently placing the spawning pupation hatching device in another clean insect cage, marking the spawning date, and covering the cup mouth with a spun yarn net;
(4) placing a pupation and egg laying hatching device with 10-15L ima beans in the original thrips breeding cage, and allowing the thrips imago to lay eggs on L ima beans;
(5) repeating the operation in the step (3) after spawning for 2 days;
(6) repeating the operation in the step (4);
(7) the thrips eggs laid on L ima beans are hatched and then directly raised on L ima beans, and are used for experiments after the thrips eggs are developed to a certain age;
(8) before the thrips pupate, paving a layer of fine soil at the bottom of a spawning pupation hatching device, and waiting for the thrips to fall into the soil and pupate;
(9) transferring the thrips pupae and fine soil at the bottom of the cup to a western flower thrips eclosion device;
(10) rising from the bottom to the top of the device after the thrips eclosion;
(11) collecting the top adult thrips, transferring the top adult thrips to a thrips adult placing cage, and continuously performing the operations in the steps (1) - (6);
(12) continuing to operate according to steps (7) - (11).
Preferably, the laboratory conditions in step (2) are: the temperature is 21 +/-2 ℃, and the humidity is 60%.
Preferably, the insect cage is a hollow structure with an opening at one end and a sealing plate sealing the opening; the front side surface of the insect cage is provided with a sleeve which is communicated with the inside of the insect cage, and the position of the sleeve, which is close to the insect cage, is tied up by a cotton rope to seal the sleeve.
Preferably, the spawning pupation hatching device comprises a cup body, a filter screen and a cup bottom, wherein the cup body is of a hollow structure with openings at the upper end and the lower end, and the filter screen is arranged in the cup body and is close to the lower end of the cup body; the cup bottom is of a hollow structure with an opening at the upper end, the upper end of the cup bottom is detachably connected to the lower end of the cup body, and sandy soil is placed in the hollow structure of the cup bottom.
Preferably, the eclosion device comprises an eclosion cylinder and a collecting bottle, the eclosion cylinder is of a hollow structure with an opening at the lower end and a connecting hole at the upper end, and the lower end of the eclosion cylinder can be detachably connected with the cup bottom; the bottle mouth of the collecting bottle is detachably connected with the connecting hole.
Preferably, the insect cage is rectangular, one end of the sealing plate is hinged to the insect cage, and the other end of the sealing plate is connected to the insect cage through mutually matched buckles.
Preferably, the size of the insect cage is 30cm × 30cm × 20 cm.
Preferably, the upper end of the cup bottom is in uniform threaded connection with the lower end of the cup body, the lower end of the eclosion cylinder is in uniform threaded connection with the cup bottom, and the bottle mouth of the collecting bottle is in uniform threaded connection with the connecting hole.
Preferably, the cup bottom is made of opaque materials.
Preferably, the eclosion cylinder is made of opaque materials, and the insect raising cage and the collecting bottle are made of transparent materials.
Preferably, the sleeve is 100-120 meshes, and the filter screen is 40-50 meshes.
Compared with the prior art, the invention has the following beneficial effects:
(1) the method provided by the invention is beneficial to breeding a large number of regular experimental thrips and plays an important role in indoor bioassay experiments.
(2) The method of the invention is convenient for taking and use, and has little damage to the thrips body when transferring the thrips.
Drawings
FIG. 1 is a schematic view of the overall structure of the insect cage of the present invention;
FIG. 2 is a schematic diagram of the overall structure of the egg-laying pupation hatching device of the present invention;
FIG. 3 is a cross-sectional view of FIG. 2;
FIG. 4 is a schematic view of the feathering apparatus of the present invention shown in connection with the bottom of a cup;
FIG. 5 is an exploded view of FIG. 4;
FIG. 6 is a cross-sectional view of FIG. 4;
description of the main reference numerals:
1-insect cage, 11-sealing plate, 12-oversleeve and 13-fastener; 2-spawning and pupating hatching device, 21-cup body, 22-filter screen, 23-cup bottom and 231-sandy soil; 3-eclosion device, 31-eclosion cylinder, 311-connecting hole, 32-collecting bottle.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. It is intended that all modifications or alterations to the methods, procedures or conditions of the present invention be made without departing from the spirit or essential characteristics thereof.
Example 1：
A method for continuously feeding thrips comprises the following steps:
(1) l cultivation of ima bean, selecting L ima bean with plump seeds, no insect population and no mildew, planting in a greenhouse flowerpot, watering and fertilizing every day until L ima bean grows to blossom and pod for use;
(2) breeding of Frankliniella occidentalis, picking up L ima bean pods 10-15, bringing the pods back to a laboratory (temperature 21 +/-2 ℃, humidity 60%), placing the pods in an insect cage (30cm × 30cm × 20cm), placing the pods in an egg-laying pupation incubation device with the bottom of diameter 5cm, mouth diameter of 8cm and height of 8cm, releasing 100 heads of Frankliniella occidentalis adults in the insect cage, and allowing Frankliniella occidentalis to take food and lay eggs on L ima beans;
(3) after the western flower thrips lay eggs for 2 days, thrips imagoes on L ima beans are driven out, L ima beans and an egg-laying pupation hatching device are taken out and are independently placed in a clean insect breeding cage, the egg laying date is marked, and the cup mouth is covered by a spun yarn net;
(4) placing a pupation and pupation hatching device with 10-15L ima beans in the original insect cage of the Frankliniella occidentalis, and allowing the Frankliniella occidentalis adults to lay eggs on L ima beans;
(5) repeating the operation in the step (3) after spawning for 2 days;
(6) repeating the operation in the step (4);
(7) after hatching, the western flower thrips laid on L ima beans are directly raised on L ima beans, and are used for experiments after the western flower thrips are developed to a certain age;
(8) before the western flower thrips pupate, a layer of fine soil is paved at the bottom of the spawning pupation hatching device, and the western flower thrips pupate after falling soil;
(9) transferring the western flower thrips pupae and the fine soil at the bottom of the cup to a western flower thrips eclosion device;
(10) rising from the bottom to the top of the device after the frankliniella occidentalis eclosion;
(11) collecting the top western flower thrips imagoes, transferring the western flower thrips imagoes to an imago breeding cage, and continuously carrying out the operations in the steps (1) to (6);
(12) continuing to operate according to steps (7) - (11).
As shown in fig. 1, an apparatus for continuously feeding thrips comprises: insect feeding cage 1, spawning pupation hatching apparatus 2 and eclosion device 3, wherein: the insect-raising cage 1 is a hollow structure with an opening at one end and the opening is sealed by a sealing plate 11; the front side surface of the insect cage is provided with a sleeve 12, the sleeve 12 is communicated with the inside of the insect cage 1, and the position of the sleeve 1 close to the insect cage 1 is tied up by a thread rope (not shown in the attached drawing) to seal the sleeve 1; in this embodiment, insect cage 1 is the rectangle, and closing plate 11 one end articulates on insect cage 1, and the other end is connected on insect cage 1 through mutually supporting buckle 14, and the hinge among the articulated mode can use prior art realizes. In this embodiment, the insect cage 1 is made of transparent material.
Referring to fig. 2-3, the spawning and pupating hatching device 2 comprises a cup body 21, a filter screen 22 and a cup bottom 23, wherein the cup body 21 is a hollow structure with openings at the upper end and the lower end, and the filter screen 22 is arranged in the cup body 21 and close to the lower end of the cup body 21; the cup bottom 23 is a hollow structure with an opening at the upper end, the upper end of the cup bottom 23 is detachably connected with the lower end of the cup body 21, and sandy soil 231 is placed in the hollow structure of the cup bottom 23; the cup bottom is made of opaque material; the moisture of the sandy soil is 15-20%.
Referring to attached 4-6, the eclosion device 3 comprises an eclosion cylinder 31 and a collecting bottle 32, the eclosion cylinder 31 is of a hollow structure with an opening at the lower end and a connecting hole 311 at the upper end, and the lower end of the eclosion cylinder 31 can be detachably connected with the cup bottom 23; the mouth of the collecting bottle 32 is detachably connected with the connecting hole 311; in this embodiment, the eclosion cylinder 31 is made of opaque material, and the collection bottle 32 is made of transparent material.
In addition, in the embodiment, the upper end of the cup bottom 23 is uniformly connected with the lower end of the cup body 21, the lower end of the eclosion cylinder 31 is connected with the cup bottom 23, and the bottle mouth of the collecting bottle 32 is uniformly connected with the connecting hole 311 in a threaded manner. The oversleeve 12 is 100-120 meshes, and the filter screen 22 is 40-50 meshes.
In conclusion, the method provided by the invention is beneficial to breeding a large number of regular and consistent experimental thrips, and plays an important role in indoor bioassay experiments; the method of the invention is convenient for taking and use, and has little damage to the thrips body when transferring the thrips.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (9)
1. A method for continuously feeding thrips is characterized by comprising the following steps:
(1) l cultivation of ima bean, selecting L ima bean with plump seeds, no insect population and no mildew, planting in a greenhouse flowerpot, watering and fertilizing every day until L ima bean grows to blossom and pod for use;
(2) breeding thrips, namely picking up L ima bean pods 10-15, taking the bean pods back to a laboratory, placing the bean pods in an insect cage, placing the bean pods in an egg-laying pupation hatching device with the bottom of 5cm in diameter, the mouth of 8cm in diameter and the height of 8cm, releasing 100 heads of adult thrips into the insect cage, and allowing the thrips to take food and lay eggs on L ima beans;
(3) after the thrips lay eggs for 2 days, catching up the thrips imagoes on L ima beans, taking out L ima beans and a spawning pupation hatching device, independently placing the spawning pupation hatching device in another clean insect cage, marking the spawning date, and covering the cup mouth with a spun yarn net;
(4) placing a pupation and egg laying hatching device with 10-15L ima beans in the original thrips breeding cage, and allowing the thrips imago to lay eggs on L ima beans;
(5) repeating the operation in the step (3) after spawning for 2 days;
(6) repeating the operation in the step (4);
(7) the thrips eggs laid on L ima beans are hatched and then directly raised on L ima beans, and are used for experiments after the thrips eggs are developed to a certain age;
(8) before the thrips pupate, paving a layer of fine soil at the bottom of a spawning pupation hatching device, and waiting for the thrips to fall into the soil and pupate;
(9) transferring the thrips pupae and the fine soil at the bottom of the cup to a thrips eclosion device;
(10) rising from the bottom to the top of the eclosion device after the eclosion of the thrips;
(11) collecting the top adult thrips, transferring the top adult thrips to a thrips adult placing cage, and continuously performing the operations in the steps (1) - (6);
(12) continuing to operate according to steps (7) - (11).
2. The method for continuously feeding thrips according to claim 1, wherein the laboratory conditions in the step (2) are: the temperature is 21 +/-2 ℃, and the humidity is 60%.
3. The method for continuously feeding thrips according to claim 1, wherein said insect cage is a hollow structure with an open end and a sealing plate sealing the open end; the front side surface of the insect cage is provided with a sleeve which is communicated with the inside of the insect cage, and the position of the sleeve, which is close to the insect cage, is tied up by a cotton rope to seal the sleeve;
the egg-laying pupation hatching device comprises a cup body, a filter screen and a cup bottom, wherein the cup body is of a hollow structure with openings at the upper end and the lower end, and the filter screen is arranged in the cup body and is close to the lower end of the cup body; the cup bottom is of a hollow structure with an opening at the upper end, the upper end of the cup bottom is detachably connected to the lower end of the cup body, and sandy soil is placed in the hollow structure of the cup bottom;
the eclosion device comprises an eclosion cylinder and a collecting bottle, the eclosion cylinder is of a hollow structure with an opening at the lower end and a connecting hole at the upper end, and the lower end of the eclosion cylinder can be detachably connected with the cup bottom; the bottle mouth of the collecting bottle is detachably connected with the connecting hole.
4. A method for continuously feeding thrips according to claim 3, wherein said insect cage is rectangular, one end of said sealing plate is hinged to said insect cage, and the other end is connected to said insect cage by means of a snap fit.
5. A method for continuously feeding thrips according to claim 3, wherein said cage has a size of 30cm × 30cm × 20 cm.
6. A method for continuously feeding thrips according to claim 3, wherein the connection mode of the upper end of the cup bottom and the lower end of the cup body, the lower end of the eclosion cylinder and the cup bottom, and the bottle mouth of the collection bottle and the connection hole are evenly threaded.
7. A method for continuously feeding thrips according to claim 3, wherein said cup bottom is made of opaque material.
8. A method for continuously feeding thrips according to claim 3, wherein said eclosion cartridge is made of opaque material, and said insect cage and said collection bottle are made of transparent material.
9. The method for continuously feeding thrips according to claim 3, wherein said cuff is 100-120 mesh and said strainer is 40-50 mesh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010326526.4A CN111466344A (en) | 2020-04-23 | 2020-04-23 | Method for continuously feeding thrips |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010326526.4A CN111466344A (en) | 2020-04-23 | 2020-04-23 | Method for continuously feeding thrips |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113273547A (en) * | 2021-05-24 | 2021-08-20 | 吉林农业大学 | Breeding device and breeding method for cynara scolymus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113273547A (en) * | 2021-05-24 | 2021-08-20 | 吉林农业大学 | Breeding device and breeding method for cynara scolymus |
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Inventor after: Ren Liyun Inventor after: Zhang Zhuanghong Inventor after: Wei Jiguang Inventor after: Zhu Yijian Inventor after: He Longfei Inventor after: Wang Aiqin Inventor after: Yuan Gaoqing Inventor before: Ren Liyun Inventor before: Zhang Zhuanghong Inventor before: Zhu Yijian Inventor before: He Longfei Inventor before: Wang Aiqin Inventor before: Yuan Gaoqing |