CN112205358A - Indoor population propagation method for spodoptera frugiperda - Google Patents
Indoor population propagation method for spodoptera frugiperda Download PDFInfo
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- CN112205358A CN112205358A CN202011043074.5A CN202011043074A CN112205358A CN 112205358 A CN112205358 A CN 112205358A CN 202011043074 A CN202011043074 A CN 202011043074A CN 112205358 A CN112205358 A CN 112205358A
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- 241000256251 Spodoptera frugiperda Species 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 31
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention belongs to the field of artificial propagation of insects, and particularly relates to an indoor population propagation method of spodoptera frugiperda, which comprises two stages of larva breeding, pupa breeding and adult breeding, wherein in the larva breeding stage, eggs are placed on wheat tender leaves of a wheat tender seedling tray for hatching, the wheat tender seedling tray is replaced once when the eggs are hatched to 3-4 instars, and the spodoptera frugiperda is hatched into pupae at the root of the wheat and then transferred to an adult breeding device for breeding.
Description
Technical Field
The invention belongs to the field of artificial propagation of insects, and particularly relates to an indoor population propagation method of Spodoptera frugiperda.
Background
Spodoptera frugiperdaSpodoptera frugiperda(Smith) belongs to the Lepidoptera noctuidae family, and is a migratory pest with extremely strong migratory ability. The insect is native to the tropical and subtropical regions of the americas, was first discovered in africa in 2016 (1) and rapidly spread to approximately 44 countries in south of sahara, causing severe yield loss in corn. Move into india in 2018 in 5 months, and further spread to southeast asian countries such as srilanka, bangla, thailand, burma, etc. A large insect source is formed in Burma in the end of 2018, and is found for the first time in 1 month in 2019 in Jiangcheng county in Yunnan province of China, so that the insect has been migrated and spread to 1518 counties in 26 province of China, and great influence is caused on food safety in China.
The Spodoptera frugiperda bred indoors all year round can be cultured into sensitive populations sensitive to medicaments due to no contact with pesticides for a long time, and research materials are provided for researching the drug resistance of insects and mechanisms generated by the insects and biological control methods (such as toxicity measurement of hot biocontrol bacteria in the current research) so as to provide important theoretical basis for guiding prediction and comprehensive prevention and control of the insects in the field. At present the indoor raising device of little spaces such as multi-purpose plastic box of raising of meadow spodoptera raises in the artificial climate case, and host plant uses maize leaf, kernel of corn or artificial feed etc. to be main more, can appear following problem at the raising in-process:
1) the spodoptera frugiperda who raises in little spaces such as plastic box is owing to be in the artificial feeding condition for a long time, especially the spodoptera frugiperda who feeds with the fodder, and the degeneration phenomenon appears in the population easily, mainly has: the survival rate is reduced, the development duration is increased, the service life of adults is short, the reproductive capacity is reduced and the like;
2) the Spodoptera frugiperda is fed by the self-planted corn leaves which do not use the insecticide, and the corn is not easy to obtain in partial areas due to the seasonality of the growth of the corn, and the growth speed of the corn is low, so that the time of at least 3 weeks is needed from the germination acceleration of the corn seeds to the availability of the corn leaves, and the period is long;
3) when the corn is fed by fresh corn grains purchased in the market, firstly, chemical agents cannot be guaranteed not to be applied, so that the indoor population can generate drug resistance, and the sensitive population is not easy to culture;
when the corn leaves, the corn kernels and the feed are used for breeding spodoptera frugiperda in small spaces such as plastic boxes, the spodoptera frugiperda is easy to lose water and wither, dry and mildew, and the like, so that the leaves, the corn kernels or the feed need to be frequently replaced in the larva breeding process (generally, the leaves are replaced every 1-2 days, and the leaves are replaced every half day when the water loss is serious), a large amount of time needs to be consumed, and frequent operation easily causes interference on the growth and development of the larvae.
Disclosure of Invention
The invention mainly aims to provide an indoor population breeding method for spodoptera frugiperda, which aims to solve the problems in the background technology.
In order to achieve the above purpose, the invention provides the following technical scheme:
an indoor population breeding method of Spodoptera frugiperda comprises two stages of larva breeding, pupa breeding and adult breeding, and is characterized in that the larva breeding stage comprises the following steps:
(1) and (3) wheat tender seedling cultivation: preparing wheat seeds and a seedling raising plate, soaking the seeds for 8-12 hours by using clear water, then flatly paving the soaked seeds on an upper layer plate of the seedling raising plate, putting the clear water in a lower layer plate, and keeping the water not to be diffused through the upper layer plate until the tender leaves grow to 8-15 cm;
(2) laying eggs: reducing the water content in the cultured seedling raising plate of the wheat young seedling to 2-3mm, keeping the water content, placing the seedling raising plate in an insect cage, placing the spodoptera frugiperda egg blocks on the wheat young leaves, and allowing the egg grains to freely hatch;
(3) pupating: and (3) after 6-7 days of incubation of the larvae, when the larvae are 3-4 instars, replacing the seedling raising trays for wheat tender seedlings, reducing the number of spodoptera frugiperda larvae in each seedling raising tray, removing water in the seedling raising trays, enabling the larvae of 4-5 instars to penetrate into the lower tray, taking the roots of the wheat, and pupating the roots.
Furthermore, the length and width of each seedling raising plate is 33cm x 25cm, 200-300 eggs can be placed in each seedling raising plate during egg laying, and 50 larvae of 4 years old are placed in each plate during pupation.
Further, the indoor temperature is not lower than 20 ℃, and the artificial climate box can be placed in winter for feeding, and the conditions are as follows: the illumination period L is 16h to 8h, the temperature is 27 +/-1 ℃, and the humidity is 70-80%.
Further, in the pupa and adult breeding stage, pupae pupated at the same day of the wheat root are picked out, after male and female are distinguished, the pupae and the male are placed into an adult breeding device according to the male-female ratio of 3:3, after the adult eclosion, a group of absorbent cotton balls are taken and dipped with 10% of honey water, the cotton balls are placed into the adult breeding device by tweezers, and after the adult spawns, egg masses in the adult breeding device are carefully cut off by scissors for incubation.
Furthermore, the insect-raising cage comprises a frame, wherein the frame consists of 12 rods, the length, the width and the height of the frame are 75cm, 33cm and 20cm, the frame is covered by a 200-mesh gauze, and hardboards or tin foil paper are placed at the bottoms of ridges.
The invention has the following advantages:
1. according to the biological habit and the characteristics of the spodoptera frugiperda larvae, the invention greatly improves the original feeding method, forms a set of feeding method suitable for maintaining the population adaptability and the sensitive population quantity of the spodoptera frugiperda, greatly simplifies the operation and saves a large amount of time.
2. The breeding device suitable for the spodoptera frugiperda larvae is used, the breeding method is matched, the breeding process is closer to the field environment, the larvae of 1-3 years old eat the wheat tender seedlings, the larvae of 4-5 years old dig into the roots of the wheat, eat the wheat roots and pupate at the roots, the indoor population degradation speed is greatly slowed down, the development fitness in the spodoptera frugiperda chamber is improved, the service life of adults is prolonged, and the reproductive capacity is improved.
3. The number of stable indoor sensitive populations can be maintained, so that the indoor sensitive populations keep unified population biological characteristics, stable sensitive populations are provided for related researches such as drug resistance of spodoptera frugiperda, conventional drug susceptibility tests usually require multiple generations of populations with the same characters, the population degeneration speed is high by using a conventional feeding method and only 20 generations can be propagated, and then a new egg block needs to be collected in a field to cause a certain error in the test, and by using the technical scheme provided by the invention, the population degeneration speed can be delayed, and the populations with the same characters can be continuously obtained to about 40 generations;
4. wheat young seedlings (living plants) are selected to feed the spodoptera frugiperda larvae, so that the time consumed by operations such as feed replacement is reduced, the manual interference of frequent feed replacement and blade replacement on the larvae is greatly reduced, the growth and development of the spodoptera frugiperda larvae are facilitated, the whole larvae are cultured to pupate, only about half a month is needed, and only one wheat young seedling raising tray needs to be replaced in the middle;
5. the wheat tender seedling is bred indoors by using the wheat seeds, the period is short, the method is simple, only the wheat tender seedling is required to be cultivated by using clear water, the method is basically not limited by seasons, and food can be provided for the greedy larvae all the year round.
Drawings
FIG. 1 is a statistical chart comparing the development history of larvae and pupae raised by two raising methods;
FIG. 2 is a statistical graph comparing the survival rate, pupation rate and emergence rate of larvae reared by two rearing methods;
FIG. 3 is a statistical chart comparing the egg laying amount of female spodoptera frugiperda bred by two breeding methods.
Detailed Description
To further clarify the objects, technical solutions and advantages of the present application, the present invention will be further described with reference to the following examples, which include, but are not limited to, the following examples. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Example 1
1. Larva raising device:
custom insect cage, frame 12 rods: length, width, height =75cm, 33cm, 20cm (adjustable as required), and is covered by a 200-mesh gauze cage to prevent larvae from escaping; hardboard or tinfoil paper of the same size can be put into the bottom of cage the inside, prevents and treats that the larva crushes the gauze.
2. And (3) wheat tender seedling cultivation:
purchasing wheat seeds and matched seedling raising trays (the length and the width are about 33cm and 25 cm), and putting 3 seedling raising trays into one breeding cage;
soaking wheat in clear water for about 8 h;
the seedling raising plate is divided into an upper layer and a lower layer, clear water is placed in the lower layer plate, soaked wheat seeds are placed on the upper layer plate, the wheat seeds are evenly paved in the upper layer plate, then the upper layer plate is placed in the lower layer plate, and moisture is prevented from overflowing the upper layer plate.
Placing the seedling raising plate into the rearing cage for cultivation to prevent other insects such as fruit flies and the like from eating the seedlings; after wheat seeds germinate (about 3 days from soaking to germination), roots penetrate through the small holes in the upper layer disc to the lower layer disc to absorb water; allowing the wheat seeds to grow and germinate until young leaves grow, and using the young leaves to grow to about 10cm to be used for raising Spodoptera frugiperda larvae (about 3 days are needed from germination to young leaves growing to 10 cm); clear water is continuously put into the lower layer plate in the middle, and the water surface does not overflow the upper layer plate; because only clear water is used for cultivating wheat seedlings, the wheat leaves generally do not exceed 15cm, the mesh of the upper layer disc of the seedling cultivation disc is large enough to enable 3-4 instar larvae to creep from the upper layer disc to the lower layer disc, and the wheat can also be spread flat for germination;
the tender leaves can be continuously supplied to the spodoptera frugiperda larvae for eating for about 6-8 days, and then gradually wither due to the feeding of the spodoptera frugiperda; therefore, the wheat seedlings can be raised once every 5 to 7 days according to the requirements of spodoptera frugiperda larvae, so that the sufficient and uninterrupted food sources and the freshness of the spodoptera frugiperda larvae are ensured;
3. the breeding method of spodoptera frugiperda larvae comprises the following steps:
before the spodoptera frugiperda egg blocks are placed on wheat tender leaves, a part of clear water in a lower layer disc is firstly poured, and only about 2-3mm of clear water is left to supply water to the roots of the wheat;
placing the spodoptera frugiperda egg blocks on wheat tender leaves, wherein 200-300 eggs can be placed in each plate, and allowing the eggs to automatically hatch; after the larvae are hatched, the larvae are fed on tender leaves of wheat by themselves; keeping a small amount of water (about 2-3 mm) in a lower layer plate of the seedling culture plate so as to maintain the growth requirement of wheat;
after the larvae are hatched for about 6-7 days, the larvae grow to 3-4 years old, at the moment, wheat in the seedling-raising tray gradually withers after being eaten by spodoptera frugiperda, a large amount of excrement of the spodoptera frugiperda is accumulated in the tray, and the larvae have self-killing habits after 4 years old, so that the seedling-raising tray needs to be replaced, and the number of spodoptera frugiperda larvae in each seedling-raising tray is reduced;
preparing a wheat seedling raising tray with new tender leaves, and completely pouring clean water in a lower tray; taking the fed seedling raising tray out of the feeding cage, taking out 4-instar spodoptera frugiperda larvae, and putting the larvae into a wheat seedling raising tray with new tender leaves, wherein 50 larvae are put in each tray. Besides eating wheat leaves, larvae of 4-5 years old can dig into the lower-layer disc, eat wheat roots and pupate at the roots. Clear water does not need to be supplemented into the seedling raising plate at the stage, because the old larvae can feed at the roots of the wheat, and if the clear water is added, the larvae can not breathe.
4. The breeding environmental conditions are as follows:
generally, if the indoor temperature is not lower than 20 ℃, the rearing cage (together with the wheat and spodoptera frugiperda larvae) can be kept at the room temperature for rearing;
if the room temperature is too low in winter, the artificial climate box can be placed for feeding, and the conditions are as follows: the illumination period L is 16h to 8h, the temperature is 27 +/-1 ℃, and the humidity is 70-80%.
5. Raising method for pupae and imagoes of Spodoptera frugiperda
The adult feeder is manufactured as follows: and pricking holes on the wall of the transparent plastic water cup by using an injector with a needle head so as to ventilate. The mouth of each of the two disposable transparent plastic cups is aligned to form a feeding space, one of the two disposable transparent plastic cups is used as a bottom (lower part), and the other one is used as a top (upper part); cutting a round hole at the top of the upper plastic cup, wherein the diameter of the round hole is about 2mm larger than that of the finger-shaped tube, the round piece is not completely cut off, 0.5cm is reserved to be connected with the plastic cup, and the round piece is adhered to the cup by using an adhesive tape; two transparent adhesive tapes with the width of 2cm and the length of about 3cm are respectively and longitudinally adhered to the left end and the right end of the joint of the cup mouths, and the specific manufacturing and feeding method can refer to an indoor feeding device and a feeding method for Spodoptera frugiperda in patent CN 202010279282.9.
Picking pupae pupated at the same day of the wheat root, and putting the pupae pupated at the same day into a self-made adult feeder according to a male-female ratio of 3:3 after distinguishing male and female;
after the imagoes are eclosized, a group of absorbent cotton balls are dipped in 10% of honey water and put into a plastic cup below the balls by using tweezers; to cotton ball replenishment moisture every day, regularly inspect the condition of laying eggs of female worm, if the discovery is laid eggs, then get a new adult feeder, open the round mouth at two feeder tops (the feeder of having laid eggs is under, new feeder is last), the hole is fixed to the hole, pat gently and has laid eggs the plastic cup bottom, because Spodoptera frugiperda adult has the habit of flying upwards, consequently in the adult will fly into the new feeder in the top, continue the mating and lay eggs. The egg mass on the wall of the feeder cup that has spawned is carefully cut with scissors for incubation.
Comparison of experiments
Comparing the wheat feeding method with the common corn kernel feeding method (the specific feeding method is referred to patent CN 202010279282.9), it is obvious from figure 1 that the development duration of Spodoptera frugiperda in the feeding method is obviously shorter than that of the common feeding method, wherein the generation development duration is shortened by 8 days compared with that of the common feeding method, which shows that the Spodoptera frugiperda fed by the feeding method can complete a generation more quickly. Note: asterisks indicate significant differences between the two feeding methods (P < 0.05), as follows.
As is obvious from figure 2, the breeding method of the present patent can significantly improve the survival rate, pupation rate and eclosion rate of the larvae, wherein the survival rate of the larvae is improved by 50% compared with the common breeding method, and the eclosion rate of the pupae is improved by 22.4%.
As is obvious from the figure 3, the average single-female egg laying amount of the spodoptera frugiperda female imagoes bred by the method is obviously higher than that of a common breeding method, and is improved by 89.27 percent compared with that of the common breeding method.
As shown in table 1, the lethal intermediate concentration LC50 of spodoptera frugiperda fed with corn kernels was about 1-fold higher than LC50 fed with wheat leaves, and spodoptera frugiperda fed with corn kernels may have a certain deviation in the accuracy of the new drug susceptibility test results due to the uncontrollable feed source.
TABLE 1 virulence determination for Spodoptera frugiperda 3 rd larvae using corn kernels and wheat leaves
Claims (5)
1. An indoor population breeding method of Spodoptera frugiperda comprises two stages of larva breeding, pupa breeding and adult breeding, and is characterized in that the larva breeding stage comprises the following steps:
(1) and (3) wheat tender seedling cultivation: preparing wheat seeds and a seedling raising plate, soaking the seeds for 8-12 hours by using clear water, then flatly paving the soaked seeds on an upper layer plate of the seedling raising plate, putting the clear water in a lower layer plate, and keeping the water not to be diffused through the upper layer plate until the tender leaves grow to 8-15 cm;
(2) laying eggs: reducing the water content in the cultured seedling raising plate of the wheat young seedling to 2-3mm, keeping the water content, placing the seedling raising plate in an insect cage, placing the spodoptera frugiperda egg blocks on the wheat young leaves, and allowing the egg grains to freely hatch;
(3) pupating: and (3) after 6-7 days of incubation of the larvae, when the larvae are 3-4 instars, replacing the seedling raising trays for wheat tender seedlings, reducing the number of spodoptera frugiperda larvae in each seedling raising tray, removing water in the seedling raising trays, enabling the larvae of 4-6 instars to penetrate into the lower tray, taking the roots of the wheat, and pupating the roots.
2. The indoor population propagation method for spodoptera frugiperda as claimed in claim 1, wherein the length and width of each seedling tray is 33cm x 25cm, 300-500 eggs can be placed on each seedling tray when eggs are placed, and 50 larvae of 4 ages are placed on each seedling tray when the seedling trays are changed when the larvae reach 3-4 ages.
3. The method for indoor population propagation of spodoptera frugiperda as claimed in claim 1, wherein the indoor temperature is not lower than 20 ℃, and the spodoptera frugiperda can be raised in a climatic chamber in winter, provided that: the illumination period L is 16h to 8h, the temperature is 27 +/-1 ℃, and the humidity is 70-80%.
4. The method of claim 1, wherein pupae pupated at the same day of wheat roots are picked up during the feeding stage of pupae and adults, and then placed into an adult feeder according to a male-female ratio of 3:3, after the adults eclosion, a batch of absorbent cotton balls are dipped in 10% honey water and placed into the adult feeder by tweezers, and after the adults lay eggs, the egg masses in the adult feeder are carefully cut off by scissors and incubated.
5. The method of claim 1, wherein the cage comprises a frame consisting of 12 rods, and has a length, a width and a height of 75cm x 33cm x 20cm, and is covered with a 200 mesh screen, and cardboard or tinfoil is placed on the bottom of the cage.
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| CN113728981A (en) * | 2021-09-24 | 2021-12-03 | 广东省农业科学院植物保护研究所 | Method for researching selective influence of volatile matter on oviposition behavior of spodoptera frugiperda |
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| CN113826588A (en) * | 2021-10-21 | 2021-12-24 | 广西壮族自治区农业科学院 | Indoor breeding method for spodoptera litura lateral groove bombesi |
| CN113826588B (en) * | 2021-10-21 | 2023-03-14 | 广西壮族自治区农业科学院 | Indoor breeding method for spodoptera litura lateral groove bombesi |
| CN114557321A (en) * | 2022-03-08 | 2022-05-31 | 广东海洋大学 | Method for breeding Spodoptera frugiperda in large quantity by taking pasture as food |
| CN114711198A (en) * | 2022-04-27 | 2022-07-08 | 中国热带农业科学院环境与植物保护研究所 | Method for breeding Spodoptera frugiperda by wheat hydroponic seedling disk grouping |
| CN114766435A (en) * | 2022-04-27 | 2022-07-22 | 中国热带农业科学院环境与植物保护研究所 | Method for artificially feeding spodoptera frugiperda larvae by using water culture seedling tray of cereal seeds |
| CN114885902A (en) * | 2022-05-30 | 2022-08-12 | 温州科技职业学院 | Breeding method of Spodoptera frugiperda |
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