CN113826588B - Indoor breeding method for spodoptera litura lateral groove bombesi - Google Patents
Indoor breeding method for spodoptera litura lateral groove bombesi Download PDFInfo
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- 238000009395 breeding Methods 0.000 title abstract description 26
- 241000256251 Spodoptera frugiperda Species 0.000 claims abstract description 60
- 241000238631 Hexapoda Species 0.000 claims abstract description 43
- 241000209094 Oryza Species 0.000 claims abstract description 39
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 39
- 235000009566 rice Nutrition 0.000 claims abstract description 39
- 238000009331 sowing Methods 0.000 claims abstract description 6
- 238000012258 culturing Methods 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 230000000384 rearing effect Effects 0.000 claims description 15
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Abstract
The invention belongs to the technical field of natural enemy insect breeding, and particularly relates to an indoor breeding method of spodoptera litura lateral groove calluses, which comprises the following steps: (1) sowing and raising seedlings of rice seeds for later use; (2) Inoculating spodoptera frugiperda or spodoptera litura larvae on the bred rice seedlings; (3) Putting the rice seedlings together with spodoptera frugiperda larvae or prodenia litura larvae into an insect cage, and culturing indoors; (4) Inoculating the spodoptera litura lateral groove bombus female and male bombus, and parasitizing the spodoptera litura lateral groove bombus; (5) removing the spodoptera litura lateral sulcus; (6) The parasitic larvae to the parasitic old larvae in the breeding step (4) drill out the walls of the host bodies, and the silking, the cocooning and the pupation are carried out; (7) Picking out the spodoptera litura lateral groove cocoon, putting into a finger-shaped pipe, and waiting for emergence; (8) And (4) repeating the steps (1) to (7) to perform subculture on the spodoptera litura lateral sulcus. The invention realizes the indoor artificial propagation of the spodoptera litura lateral groove braconid wasps.
Description
Technical Field
The invention belongs to the technical field of natural enemy insect breeding, and particularly relates to an indoor breeding method of spodoptera litura lateral groove calluses.
Background
Spodoptera litura Fabricius, spodoptera Spodoptera frugiperda (Smith), spodoptera exigua Hubner and Spodoptera cinerea (Boisdivala) belong to Lepidoptera (Lepidotera) Noctuidae (Noctuidae), are malignant agricultural pests distributed all over the world, have the characteristics of wide distribution, poor feeding, strong fertility, high destructiveness and the like, and the fact that larvae bite the plant can cause holes or defects in plant leaves and cause death of the plant when the plant is serious, even loss of death, and great economic loss and serious social influence on agricultural production. The existing method for preventing and controlling noctuidae pests still mainly uses chemical prevention and control, but the problems of pesticide residue, ecological environment pollution, high pest resistance and the like caused by using a large amount of chemical pesticide are gradually highlighted.
The Spodoptera litura lateral groove hornet Microplitis prodenia Rao & Kurian belongs to Hymenoptera Braconidae, is the dominant parasitic wasp of Spodoptera litura, spodoptera litura, spodoptera frugiperda, spodoptera exigua and other Spodoptera frugiperda pests, and can effectively inhibit the increase of the population quantity of the pests. The bee is widely distributed in southern China and southeast Asia, the annual parasitic rate is 22.31%, the highest parasitic rate can reach 62.8%, the food intake of hosts can be rapidly reduced after the host is parasitic, the host can die finally, the damage degree of pests to host plants is greatly reduced, the continuous effect can be generated in the field, the application prospect is wide, and the quality safety of agricultural products and the safety of ecological environment are facilitated. In view of the strong pest control capability and wide development and application prospects of spodoptera litura lateral cocoon bees, domestic scholars have developed biological and application ecological researches on the bees, but at present, the researches on artificial breeding technology of the bees and patents are not found.
Disclosure of Invention
The invention aims to solve the technical problems and provides an indoor breeding method of spodoptera litura lateral groove cocoons, which solves the technical problem that the spodoptera litura lateral groove cocoons cannot be bred manually in the prior art.
The technical scheme of the invention is as follows:
an indoor breeding method of spodoptera litura lateral cocoon bee comprises the following preparation steps:
(1) Soaking rice seeds in water for accelerating germination, sowing and raising seedlings after germination, and keeping the rice seedlings for later use when the rice seedlings grow to 8-12 cm;
(2) Inoculating 1-to 2-instar spodoptera frugiperda or spodoptera litura larvae on the bred rice seedlings;
(3) Putting the rice seedlings and Spodoptera frugiperda or Spodoptera litura larvae into an insect cage, and putting the insect cage in an indoor culture chamber for culturing at the temperature of 27 +/-2 ℃ and the photoperiod of L: D =14 and at the relative humidity of 70 +/-5%;
(4) 5 pairs of eclosion-mated spodoptera litura lateral groove coids are inoculated into the insect rearing cage, meanwhile, honey water is put into the insect rearing cage to provide nutrition for the spodoptera litura lateral groove coids, so that the spodoptera litura lateral groove coids parasitize spodoptera frugiperda or spodoptera litura larvae;
(5) Removing the spodoptera litura lateral groove cocoons to other insect cages after 48 hours, and allowing the spodoptera litura lateral groove cocoons to continuously parasitize spodoptera litura or spodoptera litura larvae until the spodoptera litura lateral groove cocoons die;
(6) Continuously breeding the spodoptera frugiperda or spodoptera litura larvae parasitized in the step (4) by using rice seedlings until the spodoptera frugiperda or spodoptera litura larvae grow to 4 years old, removing the spodoptera frugiperda or spodoptera litura larvae which are not parasitized, breeding the successfully parasitized spodoptera frugiperda or spodoptera litura larvae to spodoptera litura lateral sulcus mature larvae to drill out a host wall, spinning and cocooning for pupation;
(7) Picking out the spodoptera litura lateral groove cocoon bee cocoons, putting the spodoptera litura lateral groove cocoon bee cocoons into a container, and waiting for emergence of the spodoptera litura lateral groove cocoon cocoons;
(8) And (5) repeating the steps (1) - (7) and carrying out subculture on the spodoptera litura lateral groove cocoons.
In order to shorten the germination time of rice, preferably, in the step (1) of the present invention, the rice seeds are soaked in water for 3-5 hours for germination. The method uses the rice to feed spodoptera frugiperda or spodoptera litura larvae, is more favorable for parasitizing spodoptera litura lateral sulcus and is also favorable for the growth of spodoptera litura lateral sulcus larvae.
In order to reduce the time for transplanting rice and improve the survival rate of rice seedlings, in step (1) of the invention, seedling cups with the upper caliber of 7cm, the bottom diameter of 7cm and the height of 8cm are preferably used for seeding and raising.
In order to enable the spodoptera litura lateral cocoon bee to be parasitized better and obtain a better parasitizing effect, preferably, in the step (2), the accession quantity of spodoptera frugiperda or spodoptera litura larvae is 90-110.
For convenience of large-scale feeding and operation, preferably, in the step (3) of the present invention, the specification of the insect cage is 30cm × 30cm × 30cm.
In order to provide nutrition for the spodoptera litura lateral cocoon bees and improve the parasitic number of the spodoptera litura lateral cocoon bees, the mass concentration of the honey water in the step (4) is preferably 10%, and the cotton balls are dipped with the cotton balls.
In order to increase the adult rate of the spodoptera litura lateral cocoon bee, preferably, in the step (7) of the invention, the container for containing the spodoptera litura lateral cocoon bee cocoons is a finger-shaped pipe.
Due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the spodoptera litura lateral groove cocoon bee host larvae bred by the method have high survival rate, high pupation rate and emergence rate, short larval stage and emergence time and long adult survival time, and lay a foundation for artificial mass propagation of spodoptera litura lateral groove cocoon bees.
2. The spodoptera litura lateral groove cocoon bee bred by the method is not easy to degrade in species, and can stably provide enough spodoptera litura lateral groove cocoon bee sources, so that the requirement of developing various entomology researches is met.
3. According to the method, spodoptera frugiperda or Spodoptera litura with the age of 1 to 2 is used as an intermediate host, and the parasitic characteristics of Spodoptera litura lateral sulcus cocoons are utilized to obtain the Spodoptera litura lateral sulcus cocoons in an indoor artificial environment, so that indoor artificial propagation of the Spodoptera litura lateral sulcus cocoons is realized, and a solid foundation is laid for field control application of the Spodoptera litura lateral sulcus cocoons.
Drawings
FIG. 1 is a diagram showing Spodoptera frugiperda parasitized in the third generation in example 3 of the present invention.
FIG. 2 is a graph showing third generation time-old larvae drilled out from Spodoptera frugiperda body walls in example 3 of the present invention.
FIG. 3 is a diagram showing Spodoptera frugiperda parasitized in the third generation in example 3 of the present invention.
FIG. 4 is a diagram of adult Spodoptera litura lateral groove Chouioia cunea in example 3 of the present invention.
FIG. 5 is a female-male mating chart of Spodoptera litura lateral groove cocoon bee in example 3 of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
An indoor breeding method of spodoptera litura lateral cocoon bee comprises the following steps:
(1) Soaking rice seeds in water for 3h for germination acceleration, sowing and raising seedlings by using a seedling raising square cup with an upper caliber of 7cm, a bottom diameter of 7cm and a height of 8cm after germination, and keeping the rice seedlings for later use when the rice seedlings grow to 12 cm;
(2) Inoculating 90 larvae of spodoptera frugiperda or spodoptera litura with the ages of 1 st to 2 nd on the bred rice seedlings;
(3) Putting the rice seedlings and the spodoptera litura larvae into an insect cage with the specification of 30cm multiplied by 30cm, and putting the insect cage into a room for cultivation at the temperature of 27 +/-2 ℃ and the photoperiod of L: D =14 and with the relative humidity of 70 +/-5%;
(4) 5 pairs of adult prodenia litura scolii brood imagoes which are mated after eclosion are placed in the insect rearing cage, meanwhile, honey water with the mass concentration of 10% is placed in the insect rearing cage, absorbent cotton is fully dipped and placed in the insect rearing cage, and the prodenia litura lateral sulcus brood imagoes parasitize prodenia litura larvae;
(5) Removing the spodoptera litura lateral groove cocoons to other insect cages after 50h, and continuously parasitizing spodoptera litura larvae until the larvae die;
(6) Continuously breeding the parasitized spodoptera litura larvae in the step (4) by using rice seedlings until the spodoptera litura larvae grow to 4 years old, removing the parasitized spodoptera litura larvae, breeding the parasitized spodoptera litura larvae to the spodoptera litura lateral groove coilia larvae successfully to drill out the body walls of the spodoptera litura larvae, spinning and cocooning to pupate;
(7) Picking out the spodoptera litura lateral groove cocoon, putting into a finger-shaped pipe, and waiting for emergence;
(8) And (5) repeating the steps (1) to (7) and carrying out subculture on the spodoptera litura lateral sulcus.
Example 2
An indoor raising method of spodoptera litura lateral groove braconid bees comprises the following steps:
(1) Soaking rice seeds in water for 4h for accelerating germination, sowing and raising seedlings by using a seedling raising square cup with an upper caliber of 7cm, a bottom caliber of 7cm and a height of 8cm after germination, and standing by when rice seedlings grow to 10 cm;
(2) Inoculating 100 larvae of Spodoptera frugiperda of 1-2 instars to the bred rice seedlings;
(3) Putting the rice seedlings and spodoptera frugiperda larvae into an insect cage with the specification of 30cm multiplied by 30cm, and putting the insect cage in a room for cultivation at the temperature of 27 +/-2 ℃, the photoperiod of L: D =14 and the relative humidity of 70 +/-5%;
(4) 5 pairs of adult prodenia litura lateral groove coids which are mated after eclosion are placed in an insect breeding cage, meanwhile, honey water with the mass concentration of 10% is placed in the insect breeding cage, and a cotton wool ball is dipped and fully placed in the insect breeding cage, so that the prodenia litura lateral groove coids parasitize the spodoptera frugiperda;
(5) Removing the Spodoptera litura lateral groove cocoons to other insect cages after 48 hours, and allowing the Spodoptera litura lateral groove cocoons to continuously parasitize Spodoptera frugiperda until the Spodoptera litura lateral groove cocoons die;
(6) Continuously breeding the parasitized spodoptera frugiperda in the step (4) by using rice seedlings until spodoptera frugiperda larvae grow to 4 years old, removing the non-parasitized spodoptera frugiperda larvae, breeding the parasitized spodoptera frugiperda larvae successfully until parasitic wasp larvae drill out a host body wall, spinning and cocooning for pupation;
(7) Picking out the spodoptera litura lateral groove cocoon, putting into a finger-shaped pipe, and waiting for emergence;
(8) And (5) repeating the steps (1) to (7) and carrying out subculture on the spodoptera litura lateral sulcus.
Example 3
An indoor breeding method of spodoptera litura lateral cocoon bee comprises the following steps:
(1) Soaking rice seeds in water for 5h for accelerating germination, sowing and raising seedlings by using a seedling raising square cup with an upper caliber of 7cm, a bottom diameter of 7cm and a height of 8cm after germination, and keeping the rice seedlings for later use when the rice seedlings grow to 8 cm;
(2) Inoculating 110 spodoptera frugiperda larvae of which the 1 st age is not older than 2 th age to the bred rice seedlings;
(3) Putting the rice seedlings and spodoptera frugiperda larvae into an insect cage with the specification of 30cm multiplied by 30cm, and putting the insect cage in a room for cultivation at the temperature of 27 +/-2 ℃, the photoperiod of L: D =14 and the relative humidity of 70 +/-5%;
(4) 5 pairs of adult prodenia litura lateral groove coids which are mated after eclosion are placed in an insect raising cage, meanwhile, honey water with the mass concentration of 10% is placed in the insect raising cage, and a cotton wool ball is dipped and fully placed in the insect raising cage, so that the prodenia litura lateral groove coids parasitize spodoptera frugiperda larvae;
(5) Removing the Spodoptera litura lateral groove cocoons to other insect cages after 46h, and allowing the Spodoptera litura lateral groove cocoons to continuously parasitize Spodoptera frugiperda larvae until the Spodoptera litura lateral groove cocoons die;
(6) Continuously breeding the parasitized spodoptera frugiperda larvae in the step (4) by using rice seedlings until the spodoptera frugiperda larvae grow to 4 years old, removing the parasitized spodoptera frugiperda larvae, successfully breeding the parasitized spodoptera frugiperda larvae to prodenia litura lateral sulcus mature larvae to drill out a host wall, spinning and cocooning for pupation;
(7) Picking out the spodoptera litura lateral groove cocoon, putting into a finger-shaped pipe, and waiting for emergence;
(8) And (5) repeating the steps (1) to (7) and carrying out subculture on the spodoptera litura lateral sulcus.
Comparative example 1
The difference from example 2 is: the procedure of example 1 was repeated except that the rice seeds were changed to corn seeds.
Comparative example 2
An indoor breeding method of spodoptera litura lateral cocoon bee comprises the following steps:
(1) Inoculating 100 Spodoptera frugiperda of 1-2 years bred by artificial feed into an insect rearing cage of 30cm × 30cm × 30cm, and culturing the insect rearing cage in an indoor environment with the temperature of 27 + -2 ℃, the photoperiod of L: D =14 and the relative humidity of 70 + -5%;
(2) 5 pairs of adult prodenia litura lateral groove coids which are mated after eclosion are placed in an insect raising cage, meanwhile, honey water with the mass concentration of 10% is placed in the insect raising cage, and a cotton wool ball is dipped and fully placed in the insect raising cage, so that the prodenia litura lateral groove coids parasitize spodoptera frugiperda larvae;
(3) Removing the spodoptera litura lateral groove cocoons to other insect cages after 48 hours, and allowing the spodoptera litura lateral groove cocoons to continuously parasitize spodoptera frugiperda larvae until the spodoptera litura lateral groove cocoons die;
(4) Continuously feeding the parasitized spodoptera frugiperda larvae in the step (2) by using artificial feed until the spodoptera frugiperda larvae grow to 4 years old, removing the parasitized spodoptera frugiperda larvae, successfully feeding the parasitized spodoptera frugiperda larvae to prodenia litura lateral sulcus mature larvae to drill out the walls of host bodies, spinning and cocooning for pupation;
(5) Picking out the spodoptera litura lateral groove cocoon, putting into a finger-shaped pipe, and waiting for emergence;
(6) And (5) repeating the steps (1) to (5) and carrying out subculture on the spodoptera litura lateral sulcus.
Test example:
3 generations of spodoptera litura lateral cocoon bees were continuously reared according to the methods of examples 1 to 3 and comparative examples 1 to 2, under the same conditions, adult insects collected in the field were allowed to lay eggs to eclosion adult insects as the first generation, and then were continuously reared for 3 generations, with the results shown in tables 1 and 2.
Survival rate of host larvae = number of larvae at 4 instars/total number of larvae inoculated × 100%
Pupation rate = pupation number/parasitic larva number 100%
Eclosion rate = eclosion insect count/pupation insect count 100%
TABLE 1 Effect of the present invention on the survival rate, pupation rate and emergence rate of larvae of Spodoptera litura lateral sulcus
Note: different lower case letters in table 1 indicate significant differences between different feeding methods of the same generation (p < 0.05).
From the data in table 1, it can be seen that there is no difference significance (p > 0.05) in the survival rate, pupation rate, and emergence rate of spodoptera litura lateral-groove wasps of examples 1-3, and when spodoptera litura lateral-groove wasps are bred by the methods of examples 1-3 and comparative example 1, the survival rate, pupation rate, and emergence rate of host larvae of examples 1-3 are higher than those of comparative example 1, which shows that the spodoptera litura or spodoptera litura larvae edible plants have significant influence on the survival rate, pupation rate, and emergence rate of spodoptera litura lateral-groove wasps, and when the spodoptera litura or spodoptera litura larvae edible plants are changed, the survival rate, pupation rate, and emergence rate of spodoptera litura lateral-groove wasps are significantly influenced, and that the spodoptera litura seedling feeding or feeding of spodoptera litura seedlings of the invention is favorable for improving the survival rate, pupation rate, emergence rate, and emergence rate of spodoptera litura lateral-groove wasps. The differences between examples 1-3 and comparative example 2 are significant (p < 0.05), which indicates that the spodoptera frugiperda or spodoptera litura larvae are fed with artificial feed, when spodoptera litura lateral cocoon bee parasitizes on the spodoptera frugiperda or spodoptera litura larvae, the survival rate, pupation rate and eclosion rate of the spodoptera litura lateral cocoon bee larvae are reduced, indicates that the food of the spodoptera frugiperda or spodoptera litura larvae influences the physique of the spodoptera frugiperda or spodoptera litura larvae, and also can cause the survival rate, pupation rate and eclosion rate of the spodoptera litura lateral cocoon bee larvae to be reduced when the physique of the spodoptera frugiperda or spodoptera litura larvae is changed.
As can be further seen from the data in table 1, when the spodoptera litura lateral sulcus wasps are bred by the method in examples 1 to 3, the survival rate, pupation rate and emergence rate of the spodoptera litura lateral sulcus wasps in examples 1 to 3 are not significantly different (p is greater than 0.05), which indicates that the spodoptera litura lateral sulcus wasps are not easy to deteriorate in species, and can stably provide enough sources of the spodoptera litura lateral sulcus wasps, thereby meeting the requirements for developing various entomology researches.
TABLE 2 Effect of the invention on the Law stage, emergence time and adult survival time of Laoderma litura lateral groove Chouioia larvae of different generations
Note: different lower case letters in table 2 indicate significant differences between different feeding methods of the same generation (p < 0.05).
From the data in table 2, it can be seen that the spodoptera litura lateral groove wasp larvae have no difference significance (p > 0.05) in the course of period, emergence time and adult time of the larvae, when the spodoptera litura lateral groove wasps are bred by the methods of examples 1-3 and comparative example 1, the examples 1-3 have difference significance (p < 0.05) with the comparative example 1, and the examples 1-3 are superior to the comparative example 1 in the course of period, emergence time and adult time of the host larvae, which shows that the spodoptera litura or spodoptera litura larvae edible plants have significant effects on the spodoptera litura lateral groove wasp larvae period, emergence time and adult time, when the spodoptera litura or spodoptera litura edible plants are changed, the spodoptera litura lateral groove wasp larvae period, emergence time and adult time are significantly affected, which shows that the spodoptera litura lateral groove wasp larvae period, emergence time and adult time are significantly affected when the spodoptera litura edible plants are changed, which shows that the seedling feeding or spodoptera litura lateral groove wasps are beneficial to shorten the feeding period, and shorten the cost of the spodoptera litura. The differences between examples 1-3 and comparative example 2 are significant (p < 0.05), which shows that spodoptera frugiperda or spodoptera litura larvae are fed with artificial feed, when spodoptera litura lateral cocoon bee parasitizes on spodoptera frugiperda or spodoptera litura larvae, the spodoptera litura lateral cocoon larvae have the course period, the emergence time is shortened, and the adult survival time is prolonged, which shows that the food of spodoptera frugiperda or spodoptera litura larvae influences the constitution, and when the constitution of spodoptera frugiperda or spodoptera litura larvae changes, the course period, the emergence time and the adult survival time of the spodoptera litura lateral cocoon bee larvae are also influenced.
From the data in table 2, it can be seen that when the spodoptera litura lateral cocoon bee is bred by the method in examples 1-3, the spodoptera litura lateral cocoon bee larvae have no significant difference (p is more than 0.05) in the calendar stage, emergence time and emergence time, which indicates that the spodoptera litura lateral cocoon bee species is not easy to degrade, the life parameters are superior to those of the existing feed breeding, and mass, year-round and subculture breeding can be realized.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (7)
1. An indoor raising method for spodoptera litura lateral cocoon bee is characterized by comprising the following steps:
(1) Soaking rice seeds in water for accelerating germination, sowing and raising seedlings after germination, and keeping the rice seedlings for later use when the rice seedlings grow to 8-12 cm;
(2) Inoculating Spodoptera frugiperda of 1-2 years old to the bred rice seedlings;
(3) Putting the rice seedlings and spodoptera frugiperda into an insect cage, and culturing the insect cage indoors at the temperature of 27 +/-2 ℃ and the photoperiod of L: D =14 and at the relative humidity of 70 +/-5%;
(4) 5 pairs of eclosion-mated spodoptera litura lateral groove coids are inoculated into the insect rearing cage, meanwhile, honey water is put into the insect rearing cage to provide nutrition for the spodoptera litura lateral groove coids, so that the spodoptera litura lateral groove coids parasitize the Spodoptera frugiperda;
(5) 48 5363 and removing Spodoptera litura lateral cocoon bee to other insect cages after h, and allowing the Spodoptera litura lateral cocoon bee to continuously parasitize Spodoptera frugiperda until the Spodoptera litura is dead;
(6) Continuously raising the parasitized spodoptera frugiperda in the step (4) by rice seedlings until the spodoptera frugiperda grows to be 4-year old, removing the parasitized spodoptera frugiperda, successfully raising the parasitized spodoptera frugiperda to spodoptera litura lateral cocoon bee mature larvae to drill out a host wall, spinning, cocooning and pupating;
(7) Picking out the spodoptera litura lateral groove cocoon, putting into a container, and waiting for emergence;
(8) And (5) repeating the steps (1) - (7) and carrying out subculture on the spodoptera litura lateral groove cocoons.
2. The indoor rearing method of spodoptera litura lateral cocoon bee as claimed in claim 1, characterized by comprising: in the step (1), the rice seeds are soaked in water for 3-5h to accelerate germination.
3. The indoor rearing method of spodoptera litura lateral cocoon bee as claimed in claim 1, characterized by comprising: in the step (1), seedling raising cups with the upper caliber of 7cm, the bottom diameter of 7cm and the height of 8cm are used for seeding and raising seedlings.
4. The indoor rearing method of spodoptera litura lateral cocoon bee as claimed in claim 1, characterized by comprising: in the step (2), the inoculation amount of Spodoptera frugiperda is 90-110.
5. The indoor rearing method of spodoptera litura lateral cocoon bee as claimed in claim 1, characterized in that: in the step (3), the specification of the insect cage is 30cm multiplied by 30cm.
6. The indoor rearing method of spodoptera litura lateral cocoon bee as claimed in claim 1, characterized by comprising: and (4) the mass concentration of the honey water in the step (4) is 10%, and the honey water is dipped with absorbent cotton balls.
7. The indoor rearing method of spodoptera litura lateral cocoon bee as claimed in claim 1, characterized in that: in the step (7), the container for holding the spodoptera litura lateral groove cocoon bee cocoons is a finger-shaped pipe.
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| CN112205358A (en) * | 2020-09-28 | 2021-01-12 | 云南农业大学 | Indoor population propagation method for spodoptera frugiperda |
| CN113317091A (en) * | 2021-07-06 | 2021-08-31 | 吉林农业大学 | Method for breeding Spodoptera frugiperda advantage natural enemy gloeosporioides |
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| CN112205358A (en) * | 2020-09-28 | 2021-01-12 | 云南农业大学 | Indoor population propagation method for spodoptera frugiperda |
| CN113317091A (en) * | 2021-07-06 | 2021-08-31 | 吉林农业大学 | Method for breeding Spodoptera frugiperda advantage natural enemy gloeosporioides |
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