CN113826587B - Indoor breeding method for adelphocoris suturalis - Google Patents
Indoor breeding method for adelphocoris suturalis Download PDFInfo
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- CN113826587B CN113826587B CN202111224798.4A CN202111224798A CN113826587B CN 113826587 B CN113826587 B CN 113826587B CN 202111224798 A CN202111224798 A CN 202111224798A CN 113826587 B CN113826587 B CN 113826587B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
Abstract
The invention belongs to the technical field of insect feeding, and particularly relates to an indoor feeding method of adelphocoris suturalis, which comprises the following steps: sowing corn seeds, and keeping corn seedlings for later use; (2) Putting corn seedlings into an insect cage, inoculating mated male and female adults of the lygus spinosus in the oviposition stage, replacing a corn seedling pot after the lygus spinosus lays eggs for 48 hours, transferring an old corn seedling pot containing the egg mass of the lygus spinosus into another insect cage, and incubating and culturing the egg mass indoors until nymphs are incubated; (4) Inoculating 2-3-instar spodoptera frugiperda larvae into an insect cage when the nymphs of the adelphocoris chinensis grow to 2 instar; (5) Feeding adult larvae of Spodoptera frugiperda with 4-5 years old when the nymphs of the stinkbug grow to 4 years old; (6) And (5) repeating the steps (1) to (5), and carrying out subculture feeding on the lateral stinkbugs. The stinkbug breeding method has the advantages that the spawning amount of adults is large, the survival rate of the adults in each development stage is high, the large-scale propagation of the natural enemy insect stinkbug is favorably realized, and the field release amount is improved.
Description
Technical Field
The invention belongs to the technical field of insect breeding, and particularly relates to an indoor breeding method for lygus lucorum.
Background
The adelphocoris spinosus (Andrallus spinosus) belongs to the family of Hemiptera Yidusamiidae, is widely distributed in tropical and warm-temperate regions of the world, including China, philippines, japan, malaysia, indonesia, vietnam, bengal and the like, is a natural predatory natural enemy of larvae of pests of Lepidoptera, coleoptera, hymenoptera and the like, and has extremely strong pest control capability and wide development and application prospects. At present, students in China rarely develop the biological and applied ecology research of the lygus lucorum, and no research and report of the artificial breeding technology of the lygus lucorum exists. In view of this, it is necessary to provide an indoor raising method of the adelphocoris suturalis.
Disclosure of Invention
The invention aims to solve the technical problems and provides an indoor breeding method of the adelphocoris chinensis.
The technical scheme of the invention is as follows:
an indoor raising method of the lateral stinkbug comprises the following steps:
(1) Soaking corn seeds in clear water for accelerating germination, sowing after germination, and growing 3-5 true leaves of corn seedlings for later use;
(2) Putting corn seedlings into an insect cage, inoculating 10 pairs of mated male and female adults of the lygus spinosus in the oviposition stage, feeding the larvae with Spodoptera frugiperda, allowing the lygus spinosus to mate and spawn freely, replacing a corn seedling pot after the lygus spinosus spawns for 48 hours, and transferring an old corn seedling pot containing the egg mass of the lygus spinosus into another insect cage;
(3) Placing the insect cage containing the egg blocks in an indoor incubator with the temperature of 27 +/-1 ℃, the photoperiod of 10D and the relative humidity of 70 +/-5% to incubate and culture the egg blocks until the nymphs hatch, and watering the corn seedlings regularly;
(4) After nymphs grow to 2 instars, inoculating spodoptera frugiperda larvae of 2 to 3 instars into the insect rearing cage;
(5) Feeding the imago with Spodoptera frugiperda larvae of 4-5 instar when nymphs grow to 4 instar;
(6) And (5) repeating the steps (1) to (5), and carrying out subculture feeding on the lateral stinkbugs.
The nymphs hatched from the lygus lucorum primarily take nutrition from eggs, the nymphs hatched from 1 st age mainly take corn seedling juice, and the corn seedling juice can provide moisture and nutrient substances for the nymphs hatched from 1 st age, so that survival, growth and development of the nymphs hatched from 1 st age are facilitated.
In order to ensure that the corn seeds germinate more quickly, in the step (1) of the invention, the corn seeds are soaked in clear water for 3-4 h and then taken out for germination acceleration.
In order to make the corn seeds germinate faster and to improve the survival rate of the nymphs, the liquid for soaking the seeds is clear water.
In order to avoid repeated secondary transplanting, the sown corn seedlings are directly transplanted into an insect cage, in the step (1), corn seeds are sown in a seedling raising pot, the sowing amount of the corn seedlings is 48-55 plants/pot, and the sowing depth is 2cm.
In order to promote the growth and development of the lygus laterosus, the number ratio of the lygus laterosus nymphs to the spodoptera frugiperda larvae of 2-3 years in the step (4) of the invention is preferably 1. When the preys are too many, the stinkbug can be discarded after killing the preys, so that food waste is caused, food is easy to rot, maggots or bacteria are bred, and the growth and development of the stinkbug are influenced; the small number of the preys and the adelphocoris suturalis can not obtain sufficient nutrition, which causes the conditions of slow growth and development of the adelphocoris suturalis, weak individuals, reduced egg laying amount and the like.
In order to promote the growth and development of the lygus laterosus, the number ratio of the lygus laterosus nymphs to the spodoptera frugiperda larvae of 4-5 years in the step (5) of the invention is preferably 1. When the preys are too many, the stinkbug can be discarded after killing the preys, so that food waste is caused, food is easy to rot, maggots or bacteria are bred, and the growth and development of the stinkbug are influenced; the small number of the preys and the adelphocoris suturalis can not obtain sufficient nutrition, which causes the conditions of slow growth and development of the adelphocoris suturalis, weak individuals, reduced egg laying amount and the like. For convenience of operation and feeding, the specification of the insect raising cage is preferably 30cm × 30cm × 30cm.
Due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the invention adopts the corn seedling as the oviposition substrate and the food-borne host plant, the corn raw material is easy to obtain, the cost is low, the preservation is easy, and the manual operation is reduced; the method for feeding the adelphocoris suturalis has the advantages of large egg laying amount, short development duration, long survival time of adults and high survival rate of adults in each development stage, is favorable for propagating a large number of natural enemy insect adelphocoris suturalis, and improves the field release amount.
2. According to the method, spodoptera frugiperda is used as a prey insect to feed the lateral lygus bugs, after subculture, the seed properties are not degraded, and indoor artificial propagation of the predatory natural enemy lateral lygus bugs is realized.
Drawings
Fig. 1 is a block diagram of a third generation temporal stinkbug egg in embodiment 1 of the present invention.
FIG. 2 is a diagram of the nymphae of the newly hatched side-stinkbug in the third generation of the example 1 of the present invention.
Fig. 3 is a diagram of the third generation time stinkbug 1-year nymph of the invention embodiment 1.
Fig. 4 is a diagram of 2-year nymphs of third generation temporal stinkbug in embodiment 1 of the invention.
Fig. 5 is a 3-year nymph map of third generation temporal stinkbug in embodiment 1 of the present invention.
Fig. 6 is a diagram of 4-year nymph of third generation temporal stinkbug in embodiment 1 of the present invention.
Fig. 7 is a diagram of adult third generation temporal stinkbug in the embodiment 1 of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example 1
(1) Soaking corn seeds in clear water for 3.5h, taking out the corn seeds for accelerating germination, sowing the corn seeds in a seedling raising basin (with the upper caliber of 14.5cm, the bottom diameter of 10cm and the height of 12.5 cm) after germination, wherein the sowing depth is 2cm, the average sowing amount of corn seedlings is 50 plants/basin, and the corn seedlings grow 3-5 true leaves for later use;
(2) Putting 1 pot of corn seedlings into an insect cage (the insect cage is 30cm multiplied by 30 cm), inoculating 10 pairs of mated male and female adult lygus laterosus in an oviposition period, feeding the adult lygus laterosus with Spodoptera frugiperda larvae, allowing the lygus laterosus to mate and spawn freely, changing a corn seedling pot after the lygus laterosus spawns for 48 hours, and transferring an old corn seedling pot containing the lygus laterosus spawn blocks into another insect cage;
(3) Placing the insect cage containing the egg blocks in an indoor hatching culture system with the temperature of 27 +/-1 ℃, the photoperiod of 14L;
(4) After the nymphs grow to 2 years, inoculating 2-3-year spodoptera frugiperda larvae into the insect rearing cage, wherein the number ratio of the nymphs of the lygus spinosus to the 2-3-year spodoptera frugiperda larvae is 1;
(5) Feeding adults with larvae of Spodoptera frugiperda of 4-5 years when the nymphs grow to 4 years, wherein the number ratio of the nymphs of the lygus spinosus to the larvae of Spodoptera frugiperda of 4-5 years is 1;
(6) And (5) repeating the steps (1) to (5), and carrying out subculture feeding on the lateral stinkbugs.
Example 2
(1) Soaking corn seeds in clear water for 3h, taking out for accelerating germination, and after germination, sowing the corn seeds in a seedling raising pot (with the upper caliber of 14.5cm, the bottom diameter of 10cm and the height of 12.5 cm), wherein the sowing depth is 2cm, the average sowing amount of corn seedlings is 48 plants/pot, and the corn seedlings grow 3-5 true leaves for later use;
(2) Putting 1 pot of corn seedlings into an insect cage (the insect cage is 30cm multiplied by 30 cm), inoculating 10 pairs of male and female adults of the lateral stinkbug which are paired and in the oviposition stage, feeding the male and female adults with Spodoptera frugiperda larvae, enabling the lateral stinkbug to mate and lay eggs freely, changing a corn seedling pot after the lateral stinkbug lays eggs for 48 hours, and transferring an old corn seedling pot containing the egg mass of the lateral stinkbug to another insect cage;
(3) Placing the insect cage containing the egg masses in an indoor hatching culture room with the temperature of 27 +/-1 ℃, the photoperiod of 14L;
(4) After the nymphs grow to 2 years, inoculating 2-3 years Spodoptera frugiperda larvae into the insect rearing cage, wherein the number ratio of the nymphs of the lateral stinkbug to the 2-3 years Spodoptera frugiperda larvae is 1;
(5) When nymphs grow to 4 th instar, feeding adults with larvae of Spodoptera frugiperda of 4-5 th instar, wherein the number ratio of the nymphs of Linus spinosus to the larvae of Spodoptera frugiperda of 4-5 th instar is 1;
(6) And (5) repeating the steps (1) to (5), and carrying out subculture feeding on the lateral stinkbugs.
Example 3
(1) Soaking corn seeds in clear water for 4h, taking out for accelerating germination, and after germination, sowing the corn seeds in a seedling raising pot (with the upper caliber of 14.5cm, the bottom diameter of 10cm and the height of 12.5 cm), wherein the sowing depth is 2cm, the average sowing amount of the corn seedlings is 55 plants/pot, and the corn seedlings grow 3-5 true leaves for later use;
(2) Putting 1 pot of corn seedlings into an insect cage (the insect cage is 30cm multiplied by 30 cm), inoculating 10 pairs of male and female adults of the lateral stinkbug which are paired and in the oviposition stage, feeding the male and female adults with Spodoptera frugiperda larvae, enabling the lateral stinkbug to mate and lay eggs freely, changing a corn seedling pot after the lateral stinkbug lays eggs for 48 hours, and transferring an old corn seedling pot containing the egg mass of the lateral stinkbug to another insect cage;
(3) Placing the insect cage containing the egg masses in an indoor hatching culture room with the temperature of 27 +/-1 ℃, the photoperiod of 14L;
(4) After the nymphs grow to 2 years, inoculating 2-3 years Spodoptera frugiperda larvae into the insect rearing cage, wherein the number ratio of the nymphs of the lateral stinkbug to the 2-3 years Spodoptera frugiperda larvae is 1;
(5) When nymphs grow to 4 th instar, feeding adults with larvae of Spodoptera frugiperda of 4-5 th instar, wherein the number ratio of the nymphs of Linus spinosus to the larvae of Spodoptera frugiperda of 4-5 th instar is 1;
(6) And (5) repeating the steps (1) to (5), and carrying out subculture on the lateral stinkbug.
Comparative example 1
The difference from example 1 is: changing the corn seedlings into rice seedlings. The rest is the same as example 1.
Comparative example 2
The difference from example 1 is: the corn seeds are changed into peanut seeds. The rest is the same as example 1.
Comparative example 3
The difference from example 1 is: changing the corn seeds into sorghum seeds. The rest is the same as example 1.
Comparative example 4
The difference from example 1 is: the number ratio of the nymphs of the adelphocoris suturalis and the larvae of the spodoptera frugiperda of 2-3 years is 1. The rest is the same as example 1.
Comparative example 5
The difference from example 1 is: the number ratio of the nymphs of the adelphocoris spinosus to the larvae of the Spodoptera frugiperda of 2-3 years is 1. The rest is the same as example 1.
Test examples
Continuously breeding 3 generations of the stinkbugs serissoides according to the breeding methods of the examples 1-3 and the comparative examples 1-5, continuously breeding the 3 generations of the stinkbugs serissoides under the same other conditions, taking the first generation of the field from the egg laying of the collected adults to the eclosion adults, and counting the egg laying amount, the period from the egg laying to the adult, the survival time of the adults and the survival rate of each development stage when the third generation is carried out, wherein the results are shown in tables 1 and 2. Wherein:
nymph survival = nymph number at 2 ages/nymph total 100%
Survival rate of old nymphs = 5/2 × 100%
Eclosion rate = eclosion number/5-instar nymph 100%
TABLE 1 Effect of the method of the invention on the oviposition amount, developmental duration and survival time of adults of the lateral stinkbug
| Egg laying amount (granule) | Development history/d | Survival time of adults/d | |
| Example 1 | 87.00±2.65a | 35.00±1.00b | 16.33±0.58a |
| Example 2 | 82.00±2.65a | 35.00±1.00b | 15.00±1.00a |
| Example 3 | 84.67±2.89a | 34.00±2.00b | 15.67±0.58a |
| Comparative example 1 | 53.00±2.00c | 41.00±1.00a | 11.00±1.00b |
| Comparative example 2 | 62.67±2.08bc | 41.00±1.73a | 9.00±1.00c |
| Comparative example 3 | 61.67±8.50bc | 39.67±2.08a | 10.00±1.00bc |
| Comparative example 4 | 59.33±8.50bc | 39.33±1.53a | 10.33±1.53bc |
| Comparative example 5 | 66.00±7.00b | 40.33±1.53a | 11.00±1.00b |
Note: the different lower case letters in Table 1 indicate significant differences between the different feeding methods (p < 0.05).
As can be seen from Table 1, there was no significant difference (p > 0.05) in the egg laying amount, the developmental stage and the adult survival time between examples 1 to 3, but significant difference (p < 0.05) from comparative examples 1 to 5. According to the data of the comparative examples 1-3, in the method, the oviposition amount of the adelphocoris suturalis on the opposite side of the oviposition substrate and the food-source host plant, the development duration is shortened, the influence on the survival time of the adult is obviously prolonged, and the oviposition amount of the adelphocoris suturalis not increased by any oviposition substrate and food-source host plant, the development duration is shortened, and the survival time of the adult is prolonged; as can be seen from the data of comparative examples 4 to 5, the amount of the Spodoptera frugiperda larvae put in the method has a significant influence on the oviposition amount, the developmental stage and the survival time of adults of the stinkbug, and the effect of the method can be obtained by not any amount of the Spodoptera frugiperda larvae put in the method. In addition, as can be seen from the data in table 1, the adelphocoris spinosus bred by the method still has excellent vital sign parameters after 3 generations of culture, which shows that the sex of the adelphocoris spinosus bred by the method is not easy to degenerate, and lays a foundation for the mass propagation of natural enemy insect adelphocoris spinosus.
Table 2. Conditions of the present invention that affect the survival rate, survival rate and emergence rate of the nymphs of the lateral stinkbug
Note: in Table 2, different lower case letters indicate significant differences between the different feeding methods (p < 0.05).
As can be seen from Table 2, it can be seen from Table 1 that there was no significant difference (p > 0.05) between examples 1-3 in terms of the survival rate of nymphs, the survival rate of old nymphs and the emergence rate, while there was a significant difference (p < 0.05) from comparative examples 1-5. According to the data of the comparative examples 1 to 3, the oviposition substrate and the food-source host plant have obvious influence on the survival rate, the survival rate and the emergence rate of the nymphs of the lateral lygus bugs, and not any oviposition substrate and food-source host plant can improve the survival rate, the survival rate and the emergence rate of the nymphs of the lateral lygus bugs; as can be seen from the data of comparative examples 4 to 5, the amount of the Spodoptera frugiperda larvae put in the method of the present invention has a significant effect on the survival rate of the nymphs of the Linesus tarsalis, the survival rate of the advanced nymphs and the emergence rate, and the effect of the present invention can be obtained with no amount of the Spodoptera frugiperda larvae put in. In addition, as can be seen from the data in table 2, after 3 generations of culture, still higher nymph survival rate, advanced nymph survival rate and emergence rate still exist, further illustrating that the present invention can complete the propagation of indoor stinkbug.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (5)
1. An indoor raising method of adelphocoris suturalis is characterized by comprising the following steps:
(1) Soaking corn seeds in clear water for accelerating germination, sowing after germination, and growing 3-5 true leaves on corn seedlings for later use;
(2) Putting corn seedlings into an insect cage, inoculating 10 pairs of mated male and female adults of the lygus spinosus in the oviposition stage, feeding the larvae with Spodoptera frugiperda, allowing the lygus spinosus to mate and spawn freely, replacing a corn seedling pot after the lygus spinosus spawns for 48 hours, and transferring an old corn seedling pot containing the egg mass of the lygus spinosus into another insect cage;
(3) Placing the insect cage containing the egg masses in an indoor hatching culture system with the temperature of 27 +/-1 ℃, the photoperiod of 14L and the relative humidity of 70 +/-5 percent to hatch the egg masses until nymphs hatch, and watering the corn seedlings regularly;
(4) After nymphs grow to 2 years, inoculating Spodoptera frugiperda larvae of 2-3 years into the insect rearing cage;
(5) Feeding the imago with Spodoptera frugiperda larvae of 4-5 instar when nymphs grow to 4 instar;
(6) Repeating the steps (1) to (5), and carrying out subculture feeding on the lateral stinkbug;
in the step (4), the number ratio of the lygus laterosus nymphs to the spodoptera frugiperda larvae of 2-3 years is 1.
2. The indoor raising method of lygus lucorum according to claim 1, characterized by: in the step (1), the corn seeds are soaked in clear water for 3-4 h and then taken out for germination acceleration.
3. The indoor raising method of adelphocoris spinosa as claimed in claim 1, wherein: in the step (1), the corn seeds are sown in a seedling raising pot, the sowing quantity of the corn seedlings is 48-55 plants/pot, and the sowing depth is 2cm.
4. The indoor raising method of adelphocoris spinosa as claimed in claim 1, wherein: in the step (5), the number ratio of the nymphs of the adelphocoris spinosa to the larvae of the 4-5 th Spodoptera frugiperda is 1.
5. The indoor raising method of adelphocoris spinosa as claimed in claim 1, wherein: the specifications of the insect cage are 30cm multiplied by 30cm.
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