CN103168751A - Expanding propagation method for aphidius gifuensis ashmeads - Google Patents
Expanding propagation method for aphidius gifuensis ashmeads Download PDFInfo
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Abstract
The invention discloses an expanding propagation method for aphidius gifuensis ashmeads. The method comprises the following steps: inoculating aphidius gifuensis ashmead females which are about to spawn on rape seedlings with myzus persicaes, conducting aphidius gifuensis ashmead cultivation, collecting the aphidius gifuensis ashmeads, and achieving expanding propagation. According to the expanding propagation method for the aphidius gifuensis ashmeads, myzus persicaes serve as hosts of aphidius gifuensis ashmead propagation, optimized aphidius gifuensis ashmead inoculating ratio, optimized inoculating opportunity, aphidius gifuensis ashmead collecting are involved, a parasitic wasp expanding propagation technology of being high-efficiency, economical and easy and convenient to apply is formed through repeated exploration and improvement, and therefore the purposes of biological preventing and treating injurious insects are achieved.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of method that expands numerous aphidius gifuensis, particularly a kind of method that expands numerous aphidius gifuensis take the cigarette aphid as the host.
Background technology
Aphid is one of insect the most disruptive on the earth.The aphid that has been found that at present always has approximately 4400 kinds of 10 sections, and wherein nearly 250 kinds is for agriculture and forestry and the serious insect of horticulture harm.Often be clustered in the positions such as blade, tender stem, bud, terminal bud, suck food water by thorn, make leaf-shrinkage, curling, lopsided, cause the mechanical damage of plant nutrition loss and organ, in addition, aphid can be propagated viral diseases of plants, and the honeydew of its secretion can grow the causal organisms such as various plants fungus and bacterium, causes the generation of plant disease and popular.Aphid also has extremely strong instinct of reproduction, the aphid of some kinds has unique and complex modes of reproduction, i.e. parthenogenesis, and such reproduction circulation is continued until whole summer, environmental condition just can breed for 1 generation in suitable every 5 days, approximately can breed many generations in 20-40 days.Therefore a female worm can produce hundreds of millions of aphids in spring after hatching.For example, it is female that cabbage aphid (Brevicoryne brassicae) can breed for 41 generations, if all survive, can reach 1.5 * 10
27Individual offspring.
For the particularly harm of economic crops of plant, people spend a large amount of manpower and materials and attempt to control causing harm of aphid due to aphid.Wherein effective measures are a lot, for example use the chemical insecticide of internal-suction type, just can kill fast aphid.In addition, occurring in nature has a large amount of Natural Enemies of Insects predations or parasitizes aphid, and some bacteriums, fungi and virus infection aphid are also arranged.The natural enemy insect kind of aphid is a lot, and ladybug, syrphus fly, aphid cocoon honeybee, food aphid cecidomyiia, aphis lion, Chrysopa and spider etc. are arranged.Wherein aphid cocoon honeybee is the parasitic enemy insect of a special parasitic aphid of class, and very remarkable to the effect of control naturally of aphid, it is bringing into play irreplaceable important function in agriculture and forestry are produced.Utilize anti-the eliminating aphis of aphid cocoon honeybee to be conducive to reduce the use of chemical pesticide, protection of the environment is kept the ecological balance and protection bio-diversity, and is significant to the agricultural sustainable development implementation.
Aphidius gifuensis Aphidius gifuensis is strong with its fecundity, and natural parasitic rate is high, and the generation cycle is short, and strong adaptability also is easy to the merits such as artificial propagation and becomes one of natural enemy insect that in Aphidiidae, value is very high.This honeybee mainly is distributed in China's Mainland, Taiwan, Korea S, the east Asias such as Japan area, natural control to the host aphid is stronger, its host range is extensive, can parasitic cigarette aphid, radish aphid, little English grain aphid, soybean aphid, cotten aphid, and eggplant without the net aphid etc., especially remarkable to the control efficiency of cigarette aphid.At vega, aphidius gifuensis is generally 20%～60%, Gao Zheke to the parasitic rate of cigarette aphid and reaches 89.16%.In recent years, aphidius gifuensis has been subject to both domestic and external showing great attention to, and it expands numerous and application has obtained carrying out widely, and has obtained in actual applications fabulous control effect.
In view of the good biological and ecological methods to prevent plant disease, pests, and erosion ability of aphidius gifuensis, sum up the efficient multiplication technique of screening, produce in a large number the natural enemy insect product, be significant for the aphid class pest in prevention and control China vegetables, gardening, agricultural, forestry.In recent years, we are in conjunction with aphidius gifuensis auxology, alimentology, physiology and the insect control is done harm to merit rating, carried out the vast propagation technological invention research of aphidius gifuensis, around numerous honeybee host screening, best inoculation proportion, connect honeybee crucial opportunity, become the sport technique segment such as honeybee collection storage, through repeatedly exploring and improving, form efficient, economic, easy parasitic wasp multiplication technique, improved parasitic wasp output, promoted the biocontrol efficacy of aphid to promote.
In recent years, see the units such as Yunnan Academy of Tobacco Agricultural Science, Yunan Tobacco Company Yuxi Branch, Qujing City Co., Tobacco Co., Yunnan Prov., Yunnan Tobacco Co., Ltd., Dali Branch, Honghe company of Yunnan Province tobacco company, Yunnan Provine Tobacco Science Inst. and expand numerous relevant patent of invention and utility model patent about aphidius gifuensis.Above-mentioned unit mainly adopts in small-sized temperature canopy, first plant tobacco, again access the cigarette aphid, access aphidius gifuensis again, and reclaim the multiplication technique route of aphidius gifuensis adult.Such invention mainly is applicable to utilize tobacco to expand numerous parasitic wasp for the carrier plant in warm canopy at south China (particularly Yunnan Province).
In the practice of expanding numerous aphidius gifuensis, we find that above-mentioned technology is unsuitable for the northern area of China, and main manifestations is: the first, the tobacco growing cycle long, connect aphid and often need the above time of 90d, the human and material resources that needs are a large amount of from being seeded into; The second, tobacco growing need to be than large space, and the individual plant tobacco can grow to 2 meters high, needs the spacing of 1*1 rice to arrange tobacco cultivation, needs larger area to expand numerous place; The 3rd, tobacco leaf has viscosity, is unfavorable for the parasitic wasp collection; The 4th, be gathered into honeybee, be unfavorable for product storage and transportation.
In sum, be badly in need of at present a kind of above shortcoming that overcomes, the suitable technology that expands numerous aphidius gifuensis in the northern area of China, thus satisfy the needs of cigarette aphid biological control in our northern agricultural production.
Summary of the invention
The purpose of this invention is to provide a kind of method that expands numerous aphidius gifuensis.
The method of the numerous aphidius gifuensis of expansion provided by the present invention specifically can comprise the steps: to inoculate aphidius gifuensis queen bee to be laid eggs with the Brassica Napus Seedling of cigarette aphid, goes out honeybee and cultivates, and collects aphidius gifuensis, realizes expanding numerous.
In described method, the ratio of the inoculation quantity of aphidius gifuensis queen bee described to be laid eggs and described cigarette aphid quantity is 1:130 to 1:190, as 1:130 to 1:160, or 1:160 to 1:190.
In one embodiment of the invention, the ratio of the inoculation quantity of aphidius gifuensis queen bee described to be laid eggs and described cigarette aphid quantity is specially 1:130,1:160 or 1:190.
In said method, describedly go out the condition of culture that honeybee cultivates and be: daytime, (6:00 is to 20:00) temperature was 25 ± 1 ℃, and night, (20:00 is to 6:00 next day) temperature was 20 ± 1 ℃, relative moisture RH65%～70%, photoperiod L: D=14h: 10h.
In said method, the time was 4-14 days, as 4-8 days or 10-14 days.
Concrete, in described method,, describedly go out the incubation time that honeybee cultivates and can be 4-8 days (as 5 days) when collecting the stiff aphid of described aphidius gifuensis when described collection aphidius gifuensis; When described collection aphidius gifuensis becomes honeybee for collecting described aphidius gifuensis, described go out incubation time that honeybee cultivates can be 10-14 days (as 11 days) in certain temperature range, aphidius gifuensis is along with rising shortening growth cycle of temperature, and therefore, the breeding cycle also can correspondingly shorten.
In said method, described Brassica Napus Seedling with the cigarette aphid satisfies following condition: on the described Brassica Napus Seedling of every strain with cigarette aphid as described in 3700-4200 head (concrete as 3704 or 4120).
In the present invention, the preparation method of described plant seedlings with the cigarette aphid comprises the steps: that inoculating female cigarette aphid to be laid eggs to described Brassica Napus Seedling becomes aphid, inoculum density is that female cigarette aphid to be laid eggs becomes aphid as described in the described Brassica Napus Seedling of every strain inoculation 50-70 head (as 50), cultivate (as cultivating 7d) as described in every strain on Brassica Napus Seedling with 3700-4200 cigarette aphid (the cigarette aphid quantity on the blade that newly grows is in the training period disregarded), obtain described Brassica Napus Seedling with the cigarette aphid.
In described method, when inoculating described female cigarette aphid wait laying eggs and becoming aphid, described Brassica Napus Seedling is the Brassica Napus Seedling of 7-8 sheet true leaf (as 7 true leaves).At this moment, described Brassica Napus Seedling is specially growth 27-30, as the Brassica Napus Seedling of growing 27 days; The described growth Brassica Napus Seedling of 27-30 days is that rape seed is after planting cultivated the Brassica Napus Seedling that obtained in 27-30 days, is denoted as the 1st day the day of planting seed.
In said method, the described growth Brassica Napus Seedling of 27 days prepares according to the method that comprises following a and b step:
A, with the planting seed of rape, cultivate to height of seedling be 10cm;
The time of described cultivation is specially 14 days, will sow day to be denoted as the 1st day;
B, step a gained Brassica Napus Seedling is transplanted in the nutritive cube that contains culture matrix, continues to cultivate to the Brassica Napus Seedling that obtains with 7-8 sheet true leaf;
Above-mentioned with step a gained Brassica Napus Seedling be transplanted to the nutritive cube that contains culture matrix (be specially according to 2 described Brassica Napus Seedlings of every alms bowl in 12cm * 12cm) and transplant,
Above-mentioned culture matrix is specially the mixture that the peat composed of rotten mosses and vermiculite mix with volume ratio 2 ︰ 1;
The time that described continuation is cultivated is specially 13 days
The condition of various described cultivations all is specially 28 ℃ ± 1 ℃ of day temperature, 22 ± 1 ℃ of nights, relative moisture 65%-70%, photoperiod L: D=14h: 10h.
In described method, described collection aphidius gifuensis specifically can be collects the stiff aphid of described aphidius gifuensis, also can be the described aphidius gifuensis of collection and becomes honeybee.
When collecting the stiff aphid of described aphidius gifuensis, carry out according to the operation of following A; When the described aphidius gifuensis of collection becomes honeybee, carry out according to the operation of following B:
A will soak in water 10-15 minute through the described Brassica Napus Seedling blade with stiff aphid that goes out after honeybee is cultivated, and the described stiff aphid of shaking simultaneously to come off, and collect described stiff aphid;
B is placed in the container of openend sealing with the described Brassica Napus Seedling blade with stiff aphid through going out after honeybee is cultivated, and described container is placed in the collection beehive, provide light above described collection beehive, after the stiff aphid of described aphidius gifuensis sprouts wings, open the opening of described container, utilize described aphidius gifuensis to become the phototaxis of honeybee, described aphidius gifuensis to become honeybee automatically to enter the collection beehive and complete collection.
In said method, described rape is specially rape variety green bright and beautiful No. one.
The method of the numerous aphidius gifuensis of expansion provided by the present invention, convenient, economical, be easy to normalizing operation, but rapid, high volume expands numerous aphidius gifuensis, reaches the purpose that the insect biology is prevented.Advantage of the present invention is: (1) is with low cost: the seeding propagation link that technology adopts, rape is general types, expands numerous matrix (vermiculite) and can repeatedly use, and only needs to get final product through solar exposure.The nutritive cube that each link adopts, gauze etc. are all low value product, easily buy and can process voluntarily.(2) space availability ratio is high: present technique is not limit and is expanded numerous space, to the production line that expands numerous natural enemy, little of easy greenhouse and plastic greenhouse, all can be used to expand numerous aphidius gifuensis natural enemy product greatly.(3) be convenient to the batch production operation: present technique relates to the links that expands numerous aphidius gifuensis, all can intensively operate, and is convenient to batch production production.(4) stiff aphid and become honeybee automatically to collect, and can be respectively collect stiff aphid for the difference of prevention and control object, become honeybee, discharge fast, save a large amount of artificial material resources.
Embodiment
The experimental technique that uses in following embodiment is conventional method if no special instructions.
In following embodiment, material used, reagent etc., if no special instructions, all can obtain from commercial channels.
In following embodiment, photoperiod Lx:Dy represents light irradiation time x hour every day, dark duration y hour, and x+y=24.
Cigarette aphid (Myzus persicae): be recorded in " treasure once. China's cigarette aphid study on prevention general introduction. Agriculture of Anhui science, 2011,39 (2): 826-827. " in a literary composition, the public can obtain from Plant Protection institute, Chinese Academy of Agricultral Sciences.Environment matters 3d can breed for 1 generation.
Aphidius gifuensis (Aphidius gifuensis): be recorded in " Bi Zhangbao; Ji Zhengrui. aphidius gifuensis Aphidius gifuensis Ashmead biological study. Jouranl of Agricultural University of Hebei; 1993; 16 (2): 1-8. " in a literary composition, the public can obtain from Plant Protection institute, Chinese Academy of Agricultral Sciences.
Expand numerous demand for satisfying, the conservation population that need set up cigarette aphid and aphidius gifuensis, the suitable conservation environmental condition of this two population is: 25 ± 1 ℃ of day temperatures, 20 ± 1 ℃ of nocturnal temperatures, relative moisture RH65%～70%, photoperiod L: D=14h: 10h.Regularly carry out rejuvenation from field acquisition original inhabitants population.
Green bright and beautiful No. one of rape variety: can buy from Beijing middle peasant hundred standing grain development in science and technology Co., Ltds.
Embodiment 1, utilize the cigarette aphid to expand the method for numerous aphidius gifuensis
One, the cultivation of seedling
1, the seedling kind is selected
Through screening, determine to expand numerous the most suitable with rape.Kind is green bright and beautiful No. one.Select color just, large, full, the regular shape of particle, homogeneous seed.
2, seedling is numerous soon
The quick propagating and cultivating condition: daytime 28 ± 1 ℃ of (6:00 is to 20:00) temperature, night 22 ± 1 ℃ of (20:00 is to 6:00 next day) temperature, relative moisture 65%～70%, photoperiod L: D=14h: 10h.
1) vernalization sowing: the seedling fostering in the sealing isolation is indoor, put into composite soil (peat composed of rotten mosses and vermiculite are with volume ratio 2 ︰ 1 mixings) in 72 hole seedling culture hole plates, be about 3/4 of every pore volume, then be sprinkled into rape seed (every hole is 4～5 seeds approximately) and cover one deck Nutrition Soil (not being advisable just to have rape seed) on seed in seedling culture hole plate, seedling culture hole plate is put into pallet and pour into suitable quantity of water with Nutrition Soil wetting evenly (must guard against water can not be too much, prevents that seed from rotting).
2) and the time-division alms bowl: seed germination and height of seedling move on to Brassica campestris L seedling in the nutritive cube that diameter is 9cm after being 10cm left and right (about 14d, will sow day be denoted as the 1st day), cultivate 2, every alms bowl with composite soil (peat composed of rotten mosses and vermiculite are with volume ratio 2 ︰ 1 mixings).
3) fast numerous guarantee: Brassica campestris L seedling can grow to 7～8 true leaves in 13d after minute alms bowl.
4) leafy carrier connects worm: when Brassica campestris L seedling grows to 7～8 true leaves, namely can be used for next link.
Two, host's expansion is numerous
1, the host selects:
Select host---the cigarette aphid of aphidius gifuensis advantage parasitism.
2, conservation is kept: i.e. the foundation of conservation population with keep.
Expand numerous demand for satisfying, need set up the conservation population of cigarette aphid, the conservation environmental condition is: daytime, (6:00 is to 20:00) temperature was 25 ± 1 ℃, and night, (20:00 is to 6:00 next day) temperature was 20 ± 1 ℃, relative moisture RH65%～70%, photoperiod L: D=14h: 10h.Regularly carry out rejuvenation from field acquisition original inhabitants population.
3, inoculation
1) selection of inoculation time
Test material: rape (a green bright and beautiful kind), cigarette aphid;
Test method: in the climatic cabinate (south of the River, Ningbo, the RXZ-500C type) interior daytime 25 ± 1 ℃ of (6:00 is to 20:00) temperature, night, (20:00 is to 6:00 next day) temperature was 20 ± 1 ℃, relative moisture RH65%～70%, photoperiod L: D=14h: under the 10h condition, cultivate the cigarette aphid of rape and later stage access.After the rape true leaf fully launched, selecting the true number of blade was that the Brassica Napus Seedling of the different phase of 2-10 is tested, 50 of every strain rape access aphids, again through 7 days continuous culture, with the aphid amount on 1 true leaf of counter random measurement, estimation aphid total amount, and calculate single leaf and carry the aphid amount.On the blade that newly grows during this period, Aphed population is disregarded.Repeat 20 times.
Result of the test: as shown in table 1, along with the true number of blade of Brassica Napus Seedling increases, it is in rising trend that the complete stool rape carries the aphid total amount, and aphid amount rising trend is remarkable in advance, and it is stable that stage keeps.Carry aspect the aphid amount at average single leaf, fluctuating range is less, fluctuates between 410-530 head/leaf.Consider production cost, raise the efficiency, produce and to adopt when 7-8 sheet true leaf fully launches, the access aphid, through the 7d continuous culture, the aphid total amount can reach the 3700-4200 head, namely maintains 4000 head/plants of rape levels, single leaf carries the aphid amount and maintains 500 left and right, and is more satisfactory.
Table 1 connects aphid time test result
2) connect the selection of host's amount
Test material: rape (a green bright and beautiful kind), cigarette aphid;
Test method: in the climatic cabinate (south of the River, Ningbo, the RXZ-500C type) interior daytime 25 ± 1 ℃ of (6:00 is to 20:00) temperature, night, (20:00 is to 6:00 next day) temperature was 20 ± 1 ℃, relative moisture RH65%～70%, photoperiod L: D=14h: under the 10h condition, cultivate the cigarette aphid of rape and later stage access.After the 7th true leaf of rape fully launched, every strain rape access aphid connect the borer population amount and is respectively 10 of every strains, 30,50,70,90,110,150, then through 7 days continuous culture, calculates total aphid amount of carrying.With the aphid amount on 1 true leaf of counter random measurement, estimation aphid total amount is calculated single leaf and is carried the aphid amount, and the aphid build is observed.On the blade that newly grows during this period, Aphed population is disregarded.Repeat 20 times.
Result of the test: connect aphid quantity Choice tests result as shown in table 2, therefrom as seen, when connecing the aphid amount and being 50 head/plants, after the 7d continuous culture, the aphid sum reaches 3980, and single leaf carries 568.9 of aphid amounts; When connecing the aphid amount and be 70 head/plants, the aphid sum reaches 4000, and single leaf carries the aphid amount and is reached for 571.7, but the aphid individuality diminishes, and aphid cocoon honeybee has not been liked parasitism and laid eggs; When connecing the aphid amount and further being elevated to 90 head/plants, the aphid sum reaches 4200, and single leaf carries the aphid amount and increases to 600.When connecing the aphid amount and raising again, the aphid sum maintains the scale of 4200 head/plants substantially, single leaf carries the worm amount and maintains higher level, except the aphid build significantly diminishes, a large amount of alatae also occurred, presented the aphid Population excessive, rape host's nutrition supply is not enough, alatae borer population amount increases, and the flight phenomenon of migrating occurs.Consider production cost, expand the factor such as numerous efficient, in productions, definite borer population amount that connects take 50 head/plants as good, also can suitably increase inoculation quantity, is advisable with 50-70 head/plant.
Table 2 connects aphid quantity and selects result of the test
4, batch expands numerous
At interval of cultivating as stated above seedling 2 weeks, saturatedly connecing worm, with its batch production that realizes relay, satisfy the prevention and control requirement, guarantee Product supply.
Three, numerous honeybee
1, the suitableeest the determining of honeybee ratio that connect
Test material: rape (a green bright and beautiful kind), cigarette aphid and aphidius gifuensis;
Test method: in the climatic cabinate (south of the River, Ningbo, the RXZ-500C type) interior daytime 25 ± 1 ℃ of (6:00 is to 20:00) temperature, night, (20:00 is to 6:00 next day) temperature was 20 ± 1 ℃, relative moisture RH65%～70%, photoperiod L: D=14h: under the 10h condition, cultivate cigarette aphid and the aphidius gifuensis of rape and later stage access.After the 7th true leaf of rape fully launches, 50 of every strain rape access aphids, again through 7 days continuous culture, this moment, aphis population quantity reached 4000 head/plants, the aphidius gifuensis queen bee that just sprouts wings is placed in mixed population 1d, after completing mating, in the honeybee aphid ratio access aphidius gifuensis queen bee (completed mating, stalwartness and waited to lay eggs) of 1:100,1:130,1:160,1:190,1:220,1:250, namely distinguish every strain Brassica campestris L seedling access 40,31,25,21,18,16 aphidius gifuensis queen bees (completed mating, stalwartness and waited to lay eggs) again.Calculate the stiff aphid quantity of aphidius gifuensis after cultivating 5d.With the stiff aphid amount on 1 true leaf of counter random measurement, the estimation total amount, and calculate single honeybee contribution rate (single honeybee contribution rate=stiff aphid sum/connect honeybee number).On the blade that newly grows during this period, stiff aphid number is disregarded.Repeat 20 times.
Result of the test: connect honeybee quantity result of the test such as table 3, therefrom visible with the increase that connects honeybee quantity, the stiff aphid quantity of aphidius gifuensis is in rising trend and tend to be steady gradually, and single honeybee contribution rate is the downward trend again that first rises.This be mainly due to aphidius gifuensis in the behavior of parasitic aphid, exist terebra to sound out process, generally will be through the repeated multiple times aphid terebra be selected, after judging that this aphid meets the filial generation nutritional need, just lay eggs in the aphid body, if aphidius gifuensis density is too high, terebra is frequent repeatedly to aphid, the mechanical damage that forms will cause more cigarette aphid dead, and the aphidius gifuensis of having laid eggs in this aphid body can not be completed growth course, has affected the formation of stiff aphid.From stiff aphid quantitative index, honeybee aphid ratio is between 1:100-160, and stiff aphid quantity maintains the level of 900 head/plants, reaches higher level; From the angle of single honeybee contribution rate, honeybee aphid ratio is the interval of 1:160-250, and single honeybee contribution rate maintains the higher level of 35 stiff aphid/honeybees.The comprehensive reality of producing when honeybee aphid ratio is 1:160, had both been brought into play the parasitic usefulness of parasitic wasp the best, and the stiff aphid quantity of formation is also maximum, is Utopian inoculation proportion.
The best inoculation proportion screening test of table 3 result
Result of comprehensive above each test, expands numerous aphidius gifuensis best of breed and be: be that the Brassica Napus Seedling (7-8 sheet true leaf)+50 female cigarette aphids to be laid eggs of every strain rape inoculation of 27d becomes aphid to cultivate 7d+ to connect the honeybee ratio and become honeybee for the female aphidius gifuensis to be laid eggs of 1:160 vegetative period.This combination can be saved numerous honeybee cost greatly, can promote large-scale breeding and even the commercialization production of aphidius gifuensis.
2, the breeding cycle determines
When the indoor propagation aphidius gifuensis, from plantation host plant rape, inoculation host cigarette aphid, inoculation aphidius gifuensis to becoming bee eclosion, need altogether the 44d left and right.From the inoculation aphidius gifuensis to gathering in the crops into honeybee, whole numerous honeybee cycle is approximately 11d, sees table 4 for details.In certain temperature range, aphidius gifuensis is along with rising shortening growth cycle of temperature, and therefore, the breeding cycle also can correspondingly shorten.
The table 4 aphidius gifuensis production cycle
| Production procedure | Time (my god) |
| Cultivate Brassica campestris L seedling | 1～27 |
| Inoculation cigarette aphid | 28 |
| The Brassica campestris L seedling of cigarette aphid has been inoculated in management | 29-33 |
| The inoculation aphidius gifuensis | 34 |
| The growth of aphidius gifuensis | 35-43 |
| Collect aphidius gifuensis and become honeybee | 44 |
Four, automatically collecting
The product-collecting technology can be divided into two classes by the difference of collecting the worm attitude: a class is for collecting stiff aphid, and a class is to be gathered into honeybee, takes respectively different collection methods.Owing to being gathered into the honeybee survival life-span short, be unfavorable for the storage of natural enemy insect product, packing, long distance transportation, also be not easy to the field and discharge, so the present invention provides the method for collecting for stiff aphid simultaneously.
The method of collecting stiff aphid (carrying out in 4-8 days) after connecing honeybee is simpler, also is convenient to make the pupa card.Method is to collect semiarid rape leaf, insert in the tank that covers gauze, inundation 10～15 minutes, during light shaking, the stiff aphid of aphidius gifuensis can come off from blade, and floats on the upper strata, uses bamboo strainer to pull out and gets final product.The required time of the visual market of the stiff aphid of collection gained to the aphidius gifuensis product takes the circumstances into consideration to adjust temperature, to accelerate or the delayed development progress, controls the time that it turns into aphidius gifuensis.
As need, adult is collected, can utilize the aphidius gifuensis adult that the habit of assembling, becoming light is arranged upward, the stiff aphid that sprouts wings is soon inserted in small-sized cage together with rape leaf, cage is inverted funnel-form, encloses with lighttight black cloth, and opening is left in the top.Before collecting, opening is tightened, inserted in the collection beehive, and in one of overhung incandescent lamp.After a large amount of adult eclosions, can open incandescent lamp, utilize aphidius gifuensis to become the phototaxis of honeybee, make it to be gathered in opening part, untie the opening of tightening in good time, aphidius gifuensis becomes honeybee automatically to enter the collection beehive.
Claims (10)
1. a method that expands numerous aphidius gifuensis, comprise the steps: to inoculate aphidius gifuensis queen bee to be laid eggs with the Brassica Napus Seedling of cigarette aphid, goes out honeybee and cultivate, and collects aphidius gifuensis, realizes expanding numerous.
2. method according to claim 2, it is characterized in that: in described method, the ratio of the inoculation quantity of aphidius gifuensis queen bee described to be laid eggs and described cigarette aphid quantity is 1:130 to 1:190.
3. method according to claim 1 and 2 is characterized in that: in described method, described to go out the incubation time that honeybee cultivates be 4-14 days.
4. arbitrary described method according to claim 1-3, it is characterized in that: described Brassica Napus Seedling with the cigarette aphid satisfies following condition: on the described Brassica Napus Seedling of every strain with 3700-4200 described cigarette aphid.
5. arbitrary described method according to claim 1-6, it is characterized in that: in described method, described Brassica Napus Seedling with the cigarette aphid is to obtain according to the method that comprises the steps: inoculate female cigarette aphid to be laid eggs to described Brassica Napus Seedling and become aphid, inoculum density is that 50-70 female cigarette aphid described to be laid eggs of the described Brassica Napus Seedling inoculation of every strain becomes aphid, be cultured on the described Brassica Napus Seedling of every strain with 3700-4200 cigarette aphid, obtain described Brassica Napus Seedling with the cigarette aphid.
6. method according to claim 5 is characterized in that: in described method, when inoculating described female cigarette aphid wait laying eggs and becoming aphid, described Brassica Napus Seedling is the Brassica Napus Seedling of 7-8 sheet true leaf.
7. according to claim 5 or 6 described methods is characterized in that: in described method, when inoculating described female cigarette aphid wait laying eggs and becoming aphid, described Brassica Napus Seedling is the growth Brassica Napus Seedling of 27-30 days; The described growth Brassica Napus Seedling of 27-30 days is that rape seed is after planting cultivated the Brassica Napus Seedling that obtained in 27-30 days, is denoted as the 1st day the day of planting seed.
8. arbitrary described method according to claim 1-7 is characterized in that: in described method, described collection aphidius gifuensis is for collecting the stiff aphid of described aphidius gifuensis, or collects described aphidius gifuensis and become honeybee.
9. method according to claim 8 is characterized in that: the stiff aphid of the described aphidius gifuensis of described collection is following A; It is following B that the described aphidius gifuensis of described collection becomes honeybee:
A will soak in water 10-15 minute through the described Brassica Napus Seedling blade with stiff aphid that goes out after honeybee is cultivated, and the described stiff aphid of shaking simultaneously to come off, and collect described stiff aphid;
B is placed in the container of openend sealing with the described Brassica Napus Seedling blade with stiff aphid through going out after honeybee is cultivated, and described container is placed in the collection beehive, provide light above described collection beehive, after the stiff aphid of described aphidius gifuensis sprouts wings, open the opening of described container, utilize described aphidius gifuensis to become the phototaxis of honeybee, described aphidius gifuensis to become honeybee automatically to enter the collection beehive and complete collection.
10. arbitrary described method according to claim 1-9, it is characterized in that: described rape is green bright and beautiful No. of rape variety.
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