CN103168751A - Expanding propagation method for aphidius gifuensis ashmeads - Google Patents

Abstract

The invention discloses a method for multiplying aphids aphids. The method for propagating and multiplying Aphidia persicae provided by the present invention comprises the steps of: inoculating the rapeseed seedlings with A. persicae with A. persicae females waiting to lay eggs, carrying out bee culture, collecting A. persicae, and realizing Multiply. The present invention uses Aphis persicae as the host of bee reproduction, focuses on the technical links such as optimum inoculation ratio, timing of inoculation, collection of A. Quickly and massively multiply the cocoon wasps to achieve the purpose of biological prevention of pests.

Description

A kind of method for multiplying Aphids aphids

technical field

The invention belongs to the field of biotechnology, and relates to a method for multiplying the Aphidia persicae, in particular to a method for multiplying the Aphidia persicae as a host.

Background technique

Aphids are one of the most destructive pests on earth. There are about 4,400 species of aphids that have been found in 10 families, of which about 250 species are serious pests for agriculture, forestry and horticulture. Often clustered in leaves, tender stems, flower buds, terminal buds, etc., sucking plant juice through thorns, causing leaves to shrink, curl, and deform, causing plant nutrition loss and mechanical damage to organs. In addition, aphids can spread plant virus diseases, The honeydew it secretes can breed a variety of plant fungi and bacteria and other pathogenic organisms, causing the occurrence and prevalence of plant diseases. Aphids also have a very strong reproductive instinct. Some species of aphids have a unique and complex reproductive method, namely parthenogenesis. This reproductive cycle lasts until the whole summer. When the environmental conditions are suitable, one generation can be reproduced every 5 days, about 20 -40 days to be able to reproduce multiple generations. So a single female can produce hundreds of millions of aphids after hatching in the spring. For example, the cabbage aphid (Brevicoryne brassicae) is capable of producing 41 generations of females, reaching 1.5 × ¹⁰²⁷ offspring if all survive.

Due to the harm of aphids to plants, especially commercial crops, people spend a lot of manpower and material resources trying to control the damage caused by aphids. There are many effective measures, such as the use of systemic chemical insecticides, which can quickly kill aphids. In addition, there are a large number of insect natural enemies that prey on or parasitize aphids in nature, and some bacteria, fungi, and viruses infect aphids. There are many kinds of natural enemies of aphids, such as ladybugs, hoverflies, philodendrons, phiridid gall midges, aphid lions, lacewings and spiders. Among them, the aphid wasp is a kind of parasitic natural enemy insect that specializes in parasitic aphids. Its natural control effect on aphids is extremely significant, and it plays an irreplaceable important role in agricultural and forestry production. Controlling aphids by using Aphididae is beneficial to reduce the use of chemical pesticides, protect the environment, maintain ecological balance and protect biodiversity, which is of great significance to the implementation of sustainable agricultural development strategies.

Aphidius gifuensis has become one of the most valuable natural enemy insects in the family Aphidius gifuensis because of its strong fecundity, high natural parasitism rate, short generation cycle, strong adaptability and easy artificial reproduction. The bee is mainly distributed in mainland China, Taiwan, South Korea, Japan and other eastern Asian regions. It has a strong natural control over host aphids and has a wide range of hosts. Aphids, and eggplant netless aphids, etc., are particularly effective in controlling aphids. In tobacco fields, the parasitism rate of Aphids persicae to A. persicae is usually 20% to 60%, and the highest one can reach 89.16%. In recent years, Aphids aphidis has been highly concerned at home and abroad, and its propagation and application have been widely carried out, and excellent control effects have been achieved in practical applications.

In view of the excellent biocontrol ability of Aphids aphidis, it is of great significance to summarize and screen high-efficiency propagation techniques and mass-produce natural enemy insect products for the prevention and control of aphid pests in vegetables, horticulture, agriculture, and forestry in my country. In recent years, we have carried out research on the invention of large-scale multiplication technology of A. Proportion, critical timing of bee inoculation, collection and storage of adult bees, etc., after repeated exploration and improvement, an efficient, economical and simple parasitic bee propagation technology has been formed, which has increased the production of parasitoid bees and promoted the biological control ability of aphids.

In recent years, we have seen Yunnan Tobacco Agricultural Science Research Institute, Yunnan Tobacco Company Yuxi City Company, Yunnan Tobacco Company Qujing City Company, Yunnan Tobacco Company Dali State Company, Yunnan Tobacco Company Honghe State Company, Yunnan Tobacco Scientific Research Invention patents and utility model patents related to the multiplication of Aphids persicae by the institute and other units. The above-mentioned units mainly adopt the expansion technical route of planting tobacco in a small greenhouse, then inserting Aphids aphids, then inserting A. This type of invention is mainly applicable to multiplying parasitic wasps in greenhouses using tobacco as a carrier plant in southern my country (especially in Yunnan Province).

In the practice of propagating Aphids persicae, we found that the above-mentioned techniques are not suitable for the northern regions of my country. The main manifestations are: first, the tobacco growth cycle is long, and it often takes more than 90 days from sowing to inoculation of aphids, requiring a lot of manpower and material resources ; Second, the growth of tobacco requires a large space, and a single tobacco plant can grow to a height of 2 meters. To arrange tobacco cultivation with a spacing of 1*1 meter, a large area of propagation site is required; third, tobacco leaves are sticky, It is not conducive to the collection of parasitic bees; fourth, the collection of bees is not conducive to product storage and transportation.

To sum up, there is an urgent need for a technology that can overcome the above shortcomings and is suitable for the propagation of Aphids persicae in northern my country, so as to meet the needs of biological control of Aphis persica in northern agricultural production.

Contents of the invention

The purpose of the present invention is to provide a method for multiplying the Aphidius aphidis.

The method for multiplying Aphidia persicae provided by the present invention may specifically include the following steps: inoculate the rapeseed seedlings with A. persicae with the female bee of A. persicae to be laid eggs, carry out bee culture, and collect C. persicae , to achieve multiplication.

In the method, the ratio of the number of inoculated females of the Aphididae to be laid eggs to the number of the aphid is 1:130 to 1:190, such as 1:130 to 1:160, or 1: 160 to 1:190.

In one embodiment of the present invention, the ratio of the inoculated number of females of A. persicae to be laid eggs to the number of persimia is specifically 1:130, 1:160 or 1:190.

In the above method, the culture conditions for bee culture are: daytime (6:00 to 20:00) temperature 25±1°C, nighttime (20:00 to next day 6:00) temperature 20±1°C, Relative humidity RH65%～70%, photoperiod L:D=14h:10h.

In the above method, the time is 4-14 days, such as 4-8 days or 10-14 days.

Specifically, in the method, when the collection of the Aphididae is to collect the Aphidia perianth, the cultivation time for the bee culture can be 4-8 days (such as 5 days); When the collection of the Aphids persicae is to collect the adult bees of the A. persicae, the cultivation time for the bee culture can be 10-14 days (such as 11 days) in a certain temperature range, and the cocoons of A. As the temperature rises, the bee's development cycle shortens, so the reproductive cycle will also shorten accordingly.

In the above method, the rapeseed seedlings with aphids meet the following conditions: each plant of the rapeseed seedlings bears 3700-4200 (specifically, 3704 or 4120) of the rapeseeds.

In the present invention, the preparation method of the plant seedlings with aphids includes the following steps: inoculate the rapeseed seedlings with female aphids to be laid eggs, and the inoculation density is 50-70 per plant of the rapeseed seedlings. head (such as 50) of the female aphids to be laid eggs, cultured (such as cultured for 7d) until each rape seedling has 3700-4200 heads of aphids (on the newly grown leaves during the cultivation period) negligible the number of aphids), to obtain the rapeseed seedlings with aphids.

In the method, when the female aphids to be laid eggs are inoculated, the rapeseed seedlings are rapeseed seedlings with 7-8 true leaves (such as 7 true leaves). At this time, the rape seedlings are specifically 27-30 days of growth, such as rape seedlings of 27 days of growth; Diary for Day 1.

In the above method, the rapeseed seedlings grown for 27 days are prepared according to the method comprising the following steps a and b:

A, the seed sowing of rapeseed is cultivated to a seedling height of 10cm;

The time of described cultivating is specifically 14 days, and the sowing diary is regarded as the first day;

b. Transplanting the rapeseed seedlings obtained in step a into a nutrient bowl containing the culture medium, and continuing to cultivate until the rapeseed seedlings with 7-8 true leaves are obtained;

Transplanting the rapeseed seedlings obtained in step a above into a nutrient pot (12cm×12cm) containing a culture medium is specifically transplanting 2 rapeseed seedlings per pot,

The above-mentioned culture substrate is specifically a mixture of peat and vermiculite in a volume ratio of 2:1;

The time to continue cultivating is specifically 13 days

The cultivation conditions mentioned above are specifically the daytime temperature of 28°C±1°C, nighttime temperature of 22±1°C, relative humidity of 65%-70%, and photoperiod L:D=14h:10h.

In the method, the collection of the A. persicae may specifically be the collection of the A. persicae dead aphid, or the collection of the A. persinum adult bee.

When collecting the dead aphids of the aphids, proceed according to the following operation of A; when collecting the aphids of the aphids, proceed according to the operation of the following B:

A Soak the rapeseed seedling blades with aphids cultured by the bees in water for 10-15 minutes, and shake at the same time to shed the aphids, and collect the aphids;

B put the rapeseed seedling blade with the scorpion aphid after the bees have been cultured in the container with the open end closed, and place the container in the beehive, provide bright light above the beehive, and wait for the beehive After the dead aphids of A. persicae have emerged, open the opening of the container, and utilize the phototaxis of the adult C. persicae, and the adult bees of A. persiminae automatically enter the beehive to complete collection.

In the above method, the rapeseed is specifically a rapeseed variety Lujin No. 1.

The method for multiplying the Aphididae provided by the present invention is convenient, economical, and easy to standardize operation, and can quickly and massively multiply the Aphidias, so as to achieve the purpose of biological prevention of pests. The advantages of the present invention are: (1) Low cost: in the seedling multiplication process adopted by the technology, rape is a commonly used variety, and the multiplication substrate (vermiculite) can be used for many times, and only needs to be exposed to sunlight. The nutritional bowls and gauze nets used in each link are all low-value products, which are easy to buy and can be processed by themselves. (2) High space utilization rate: This technology does not limit the expansion space, as large as the production line for the expansion of natural enemies, as small as simple greenhouses and plastic sheds, can be used to multiply the natural enemy products of Aphids aphids. (3) Facilitate factory operation: This technology involves every link in the expansion and propagation of Aphids persicae, which can be operated intensively and is convenient for factory production. (4) The dead aphids and adult bees can be collected automatically, and can be collected according to different prevention and control objects, and released quickly, saving a lot of manual and material resources.

Detailed ways

The experimental methods used in the following examples are conventional methods unless otherwise specified.

The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

In the following examples, the photoperiod Lx:Dy means x hours of light and y hours of darkness per day, x+y=24.

Myzus persicae: Recorded in the article "Zeng Yu. An Overview of Research on the Control of Myzus persicae in my country. Anhui Agricultural Sciences, 2011, 39(2): 826-827." The public can obtain it from the Institute of Plant Protection, Chinese Academy of Agricultural Sciences . Environmental matters 3d can breed 1 generation.

Aphidius gifuensis: recorded in "Bi Zhangbao, Ji Zhengrui. Biological research on Aphidius gifuensis Ashmead. Journal of Hebei Agricultural University, 1993,16(2):1-8." The public can download from China Acquired from the Institute of Plant Protection, Academy of Agricultural Sciences.

In order to meet the needs of expansion, it is necessary to establish a conservation population of Aphids persicae and Aphis persicae. The suitable environmental conditions for conservation of these two populations are: daytime temperature 25±1°C, night temperature 20±1°C, relative humidity RH65% ~70%, photoperiod L:D=14h:10h. Indigenous populations are regularly collected from the wild for rejuvenation.

Rapeseed variety Lvjin No.1: can be purchased from Beijing Zhongnong Baihe Technology Development Co., Ltd.

Embodiment 1, utilize the method for multiplying Aphidia persicae

1. Cultivation of seedlings

1. Selection of seedling varieties

After screening, it was determined that rapeseed was the most suitable for multiplication. The variety is Lvjin No.1. Choose seeds that are positive in color, large in size, plump, regular in shape, and uniform in texture.

2. Rapid propagation of seedlings

Rapid propagation conditions: daytime (6:00 to 20:00) temperature 28±1°C, night (20:00 to next day 6:00) temperature 22±1°C, relative humidity 65% to 70%, photoperiod L :D=14h:10h.

1) Pregermination and sowing: In a closed and isolated seedling cultivation room, put mixed soil (peat and vermiculite mixed at a volume ratio of 2:1) in a 72-hole seedling tray, about 3/4 of the volume of each hole, Then sprinkle rapeseed seeds (about 4 to 5 seeds per hole) into the seedling tray and cover the seeds with a layer of nutrient soil (it is better to just submerge the rapeseed), put the seedling tray into the tray and water it. Add an appropriate amount of water to moisten the nutrient soil evenly (avoid too much water to prevent the seeds from rotting).

2) Divide the pots in time: After the seeds germinate and the height of the seedlings is about 10cm (about 14 days, take the planting diary as the first day), move the rapeseed seedlings to a nutrient pot with a diameter of 9cm, mix soil (peat and vermiculite and Volume ratio 2:1 mixed) cultivation, 2 trees per pot.

3) Guarantee of rapid propagation: Rapeseed seedlings can grow to 7-8 true leaves within 13 days after being divided into pots.

4) Multi-leaf carrier inoculation: When rape seedlings grow to 7-8 true leaves, they can be used in the next step.

2. Expansion of the host

1. Host selection:

Select the dominant parasitic host of Aphids persicae - A. persicae.

2. Species preservation and maintenance: that is, the establishment and maintenance of conservation populations.

In order to meet the needs of expansion, it is necessary to establish a conservation population of aphids. The environmental conditions for conservation are: daytime (6:00 to 20:00) temperature 25±1°C, night time (20:00 to 6:00 the next day) Temperature 20±1℃, relative humidity RH65%～70%, photoperiod L:D=14h:10h. Indigenous populations are regularly collected from the wild for rejuvenation.

3. Vaccination

1) Selection of inoculation time

Test materials: rapeseed (Lvjin No. 1 variety), aphids;

Test method: In an artificial climate chamber (Ningbo Jiangnan, RXZ-500C type), the temperature during the day (6:00 to 20:00) is 25±1°C, and the temperature at night (20:00 to 6:00 the next day) is 20±1°C , relative humidity RH65%～70%, photoperiod L:D=14h:10h, cultivate rapeseed and aphids inserted later. After the true leaves of rapeseed are fully unfolded, rapeseed seedlings at different stages with 2-10 true leaves are selected for experimentation. Each rapeseed plant is inoculated with 50 aphids, and then cultivated continuously for 7 days, and the number of true leaves on 1 true leaf is randomly measured by a counter. The amount of aphids, estimate the total amount of aphids, and calculate the amount of aphids on a single leaf. The number of aphids on newly grown leaves during this period was not counted. Repeat 20 times.

Test results: As shown in Table 1, with the increase in the number of true leaves of rapeseed seedlings, the total amount of aphids carried by the whole rapeseed showed an upward trend. In terms of the average single leaf aphid load, the fluctuation range is small, fluctuating between 410-530 aphids/leaf. Considering the production cost and improving efficiency, it can be adopted in production that when the 7th to 8th true leaves are fully expanded, aphids can be inserted. After 7 days of continuous cultivation, the total number of aphids can reach 3700-4200, that is, it can be maintained at 4000 per plant The level of rapeseed and the amount of aphids on a single leaf are maintained at about 500 heads, which is ideal.

Table 1 The results of the time test for picking up aphids

2) Selection of the number of hosts

Test materials: rapeseed (Lvjin No. 1 variety), aphids;

Test method: In an artificial climate chamber (Ningbo Jiangnan, RXZ-500C type), the temperature during the day (6:00 to 20:00) is 25±1°C, and the temperature at night (20:00 to 6:00 the next day) is 20±1°C , relative humidity RH65%～70%, photoperiod L:D=14h:10h, cultivate rapeseed and aphids inserted later. After the seventh true leaf of rapeseed is fully unfolded, each rapeseed plant is inoculated with aphids, and the number of inoculated insects is 10, 30, 50, 70, 90, 110 and 150 per plant, and after another 7 days For continuous culture, calculate the total load of aphids. Randomly measure the amount of aphids on a true leaf with a counter, estimate the total amount of aphids, calculate the amount of aphids on a single leaf, and observe the size of aphids. The number of aphids on newly grown leaves during this period was not counted. Repeat 20 times.

Test results: The results of the selective test on the number of aphids are shown in Table 2. It can be seen that when the amount of aphids is 50 per plant, after 7 days of continuous cultivation, the total number of aphids reaches 3980, and the amount of aphids on a single leaf is 568.9; When the number of aphids was 70 per plant, the total number of aphids reached 4,000, and the amount of aphids carried on a single leaf reached 571.7, but the individual aphids became smaller, and the aphid wasps did not like to parasitize and lay eggs; the number of aphids further increased to 90 The total number of aphids reached 4200 per plant, and the number of aphids carried on a single leaf increased to 600. When the amount of aphids inoculated increases again, the total number of aphids is basically maintained at 4200 per plant, while the single leaf insect load is maintained at a relatively high level. In addition to the significantly smaller size of aphids, a large number of winged aphids also appeared. It showed that the number of aphids was too large, the nutrient supply of the rapeseed host was insufficient, the number of winged aphids increased, and the phenomenon of migrating and fleeing appeared. Taking production cost, multiplication efficiency and other factors into consideration, it is determined that the number of inoculated insects is 50 per plant, and the number of inoculated insects can be increased appropriately, and 50-70 per plant is suitable.

Table 2 Aphid number selection test results

4. Batch multiplication

The seedlings are cultivated and inoculated with the above-mentioned method at intervals of 2 weeks, so as to realize relay batch production, meet the requirements of prevention and control, and ensure the supply of products.

3. Fanfeng

1. Determination of the most suitable honeycomb ratio

Test materials: rapeseed (Lvjin No. 1 variety), aphids and aphids;

Test method: In an artificial climate chamber (Ningbo Jiangnan, RXZ-500C type), the temperature during the day (6:00 to 20:00) is 25±1°C, and the temperature at night (20:00 to 6:00 the next day) is 20±1°C , relative humidity RH65% ~ 70%, photoperiod L: D = 14h: 10h conditions, cultivate rapeseed and later inoculation of aphids and aphids. After the 7th true leaf of rapeseed was fully unfolded, 50 aphids were inserted into each rapeseed, and then cultivated continuously for 7 days. At this time, the population of aphids reached 4000 heads/plant. After the mixed population is 1d, after the mating is completed, the bee aphid ratios of 1:100, 1:130, 1:160, 1:190, 1:220, and 1:250 are inserted into the female bee of Aphidida macifera (mating has been completed, Robust and ready to lay eggs), that is, each rapeseed seedling was connected with 40, 31, 25, 21, 18, and 16 females of Aphidida immobilis (mating has been completed, robust and ready to lay eggs). After 5 days of culture, the number of Aphids aphids was counted. Randomly measure the amount of aphids on a true leaf with a counter, estimate the total amount, and calculate the contribution rate of a single bee (contribution rate of a single bee = total number of aphids/number of bees received). During this period, there were countless aphids on newly grown leaves. Repeat 20 times.

Test results: The test results of the number of received bees are shown in Table 3, from which it can be seen that with the increase of the number of received bees, the number of Aphids aphids aphids increased and gradually stabilized, and the contribution rate of single bees first increased and then decreased. This is mainly due to the process of stinging and probing of the parasitic aphids in the behavior of the Aphids aphididae. Generally, it is necessary to repeatedly sting and select the aphids to determine that the aphids meet the nutritional needs of the offspring before laying eggs in the aphids. , if the density of aphids wasps is too high, the aphids will be repeatedly stinged frequently, and the mechanical damage will cause more aphids to die, and the aphids that have laid eggs in the aphids will not be able to complete the development process, affecting Formation of dead aphids. From the perspective of the number of bee aphids, the ratio of bee aphids is between 1:100-160, and the number of bee aphids is maintained at a level of 900 heads/plant, reaching a relatively high level; from the perspective of single bee contribution rate, the ratio of bee aphids is In the interval of 1:160-250, the contribution rate of a single bee remained at a relatively high level of 35 aphids/bee. Comprehensive production practice, when the ratio of bee aphids is 1:160, not only the best parasitic performance of parasitic wasps is exerted, but also the number of dead aphids is the largest, which is the ideal ratio of bee inoculation.

Table 3 The results of the screening test for the optimal ratio of receiving bees

Based on the results of the above experiments, the best combination for the propagation of Aphids persicae is: rapeseed seedlings (7-8 true leaves) with a growth period of 27 days + 50 female adult aphids of A. 7d+The female Aphids persicae wasps waiting to lay eggs with a ratio of receiving bees of 1:160 became bees. The combination can greatly save the cost of breeding bees, and can promote the large-scale reproduction and even commercial production of the Aphidius aphidis.

2. Determination of the reproductive cycle

When breeding Aphids persicae indoors, it takes about 44 days from planting host plant Rapeseed, inoculating host A. From the time of inoculation of Aphids persicae to the harvest of adult bees, the entire bee reproduction cycle is about 11 days, see Table 4 for details. Within a certain temperature range, the developmental period of Aphidius aphidida shortens with the increase of temperature, so the reproductive period will be shortened accordingly.

Table 4 Production cycle of Aphids aphids

Production Process time (days) Cultivation of rape seedlings 1～27 Inoculation with aphids 28 Managing Rapeseed Seedlings Inoculated with Aphids 29-33 Aphids aphids 34 Development of Aphids aphids 35-43 Collecting Aphids aphids 44

4. Automatic collection

The product collection technology can be divided into two categories according to the different states of the collected insects: one is to collect dead aphids, and the other is to collect adult bees, and different collection methods are adopted respectively. Since the collected bees have a short survival life, it is not conducive to the storage, packaging, and long-distance transportation of natural enemy insect products, and it is not convenient to release them in the field. Therefore, the present invention also provides a method for collecting aphids.

The method of collecting dead aphids (4-8 days after receiving bees) is relatively simple, and it is also convenient for making pupa cards. The method is to collect semi-dry rapeseed leaves, put them into a water tank covered with gauze, soak in the water for 10-15 minutes, shake gently during this period, and the Aphids aphids will fall off the leaves and float on the upper layer. The fence can be removed. The collected aphids can adjust the temperature according to the demand time of the market for the products of the Aphididae, so as to speed up or delay the development progress, and control the time for the eclosion of the aphids to become the A.

If you need to collect adults, you can take advantage of the habit of gathering upwards and phototaxis by the adults of Aphidius persicae, and put the fast-emergent aphids together with rape leaves into a small cage, which is in the shape of an inverted funnel, surrounded by Opaque black cloth with an opening at the top. Tighten the opening before collecting, put it into the beehive, and hang an incandescent lamp above it. After a large number of adults emerge, the incandescent lamp can be turned on, and the phototaxis of the adult wasps can be used to gather them at the opening, and the tight openings can be untied in time, and the adult bees will automatically enter the beehive.

Claims (10)

1. A method for expanding and propagating Aphidia persicae, comprising the steps of inoculating the rapeseed seedlings with Aphisia persicae to inoculate the C. persicae female honeybee to be laid eggs, carrying out bee cultivation, collecting A.sia persicae, and realizing expansion complex. 2. The method according to claim 2, characterized in that: in the method, the ratio of the number of inoculated females of the Aphididae to be laid eggs to the number of the aphids is 1:130 to 1: 190. 3. The method according to claim 1 or 2, characterized in that: in the method, the culture time for the bee culture is 4-14 days. 4. The method according to any one of claims 1-3, characterized in that: the rape seedlings with aphids meet the following conditions: each rape seedling has 3700-4200 aphids . 5. The method according to any one of claims 1-6, characterized in that: in the method, the rapeseed seedlings with aphids are obtained according to the method comprising the steps of: inoculating to the rapeseed seedlings For the female aphids to be laid eggs, the inoculation density is to inoculate 50-70 female aphids to be laid in each rapeseed seedling, and cultivate until each rapeseed seedling has 3700-4200 A. nigella, to obtain the rapeseed seedlings with A. nigella. 6. The method according to claim 5, characterized in that: in the method, when inoculating the female aphids to be laid eggs, the rape seedlings are 7-8 true leaf rape seedlings. 7. The method according to claim 5 or 6, characterized in that: in the method, when inoculating the adult aphids of the female aphids to be laid eggs, the rapeseed seedlings are 27-30 days old rapeseed seedlings; The rapeseed seedlings grown for 27-30 days are rapeseed seedlings cultivated 27-30 days after the rape seeds are sown, and the date of seed sowing is recorded as the first day. 8. The method according to any one of claims 1-7, characterized in that: in the method, the collection of the Aphidia persicae is to collect the A. Bees become bees. 9. The method according to claim 8, characterized in that: the collection of the dead aphid of the aphids is as follows A; the collection of the aphids of the aphids is the following B: A Soak the rapeseed seedling blades with aphids cultured by the bees in water for 10-15 minutes, and shake at the same time to shed the aphids, and collect the aphids; B put the rapeseed seedling blade with the scorpion aphid after the bees have been cultured in the container with the open end closed, and place the container in the beehive, provide bright light above the beehive, and wait for the beehive After the dead aphids of A. persicae have emerged, open the opening of the container, and utilize the phototaxis of the adult C. persicae, and the adult bees of A. persiminae automatically enter the beehive to complete collection. 10. The method according to any one of claims 1-9, characterized in that: the rapeseed is Lujin No. 1 rapeseed variety.