CN106852301A - It is a kind of to carry out the method that aphidius gifuensis over wintering is bred using winter rape - Google Patents

It is a kind of to carry out the method that aphidius gifuensis over wintering is bred using winter rape Download PDF

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Publication number
CN106852301A
CN106852301A CN201710059739.3A CN201710059739A CN106852301A CN 106852301 A CN106852301 A CN 106852301A CN 201710059739 A CN201710059739 A CN 201710059739A CN 106852301 A CN106852301 A CN 106852301A
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CN
China
Prior art keywords
aphidius gifuensis
aphid
rape
bred
winter
Prior art date
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Pending
Application number
CN201710059739.3A
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Chinese (zh)
Inventor
钟秋瓒
张超群
邵雪莲
申昌优
肖先仪
杨庆根
陈荣华
刘毅
刘润生
饶文平
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GANZHOU BRANCH JIANGXI TOBACCO CO
JIANGXI TOBACCO LEAF SCIENCE RESEARCH INSTITUTE
Ganzhou tobacco science research institute
Original Assignee
GANZHOU BRANCH JIANGXI TOBACCO CO
JIANGXI TOBACCO LEAF SCIENCE RESEARCH INSTITUTE
Ganzhou tobacco science research institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by GANZHOU BRANCH JIANGXI TOBACCO CO, JIANGXI TOBACCO LEAF SCIENCE RESEARCH INSTITUTE, Ganzhou tobacco science research institute filed Critical GANZHOU BRANCH JIANGXI TOBACCO CO
Priority to CN201710059739.3A priority Critical patent/CN106852301A/en
Publication of CN106852301A publication Critical patent/CN106852301A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a kind of the method that aphidius gifuensis over wintering is bred, including following steps are carried out using winter rape:(1) host plant winter rape is cultivated in warmhouse booth;(2) small-sized fly net construction;(3) cigarette aphid inoculation is bred;(4) aphidius gifuensis inoculation;(5) aphidius gifuensis are bred;(6) large-scale breeding.The present invention cultivates host plant rape in warmhouse booth, builds small-sized fly net, and makes full use of cigarette aphid to the taxis of yellow rape flower, breeds the parasitic aphidius gifuensis of cigarette aphid and survives the winter, it can be ensured that next year aphidius gifuensis high-volume is supplied.The present invention solves the problems, such as that aphidius gifuensis are difficult to over wintering and breed, and realizes aphidius gifuensis Ashmaed scale and breeds and promote.

Description

It is a kind of to carry out the method that aphidius gifuensis over wintering is bred using winter rape
Technical field
The present invention relates to the technical field of pest and disease damage biological control, and in particular to one kind carries out cigarette aphid cocoon using winter rape The method that honeybee over wintering is bred.
Background technology
Cigarette aphid, Classification system is MyzusPersicae (Sulzer), also known as black peach aphid is one of tobacco primary pest.Cigarette aphid Tobacco leaf, stalk and floral axis juice are inhaled with into, larvae aphid thorn, likes intensive on leaf back or lobus cardiacus, blade is aggrieved to be occurred taking off Color spot point, serious jaundice is crispaturaed, is deformed or withered.Tender stem, bennet are aggrieved in deformity, and capsule development is abnormal or withered;This Cucumber Mosaic Virus can also be propagated outward, and influence cigarette strain is grown or triggers generation and the prevalence of virosis, causes tobacco leaf to subtract Product degrades.
At present, the preventing and treating to cigarette aphid is main based on chemical pesticide.Due to the long-term use of chemical pesticide, in leaf tobacco production Occur in that 3R problems:Residual (residue), once again resistance (resistance), wildness (resurgence).To improve tobacco leaf peace Quan Xing, protecting ecology system, in recent years, biological control has turned into the developing direction of Pest Management of Tobacco.
Aphidius gifuensis (Aphidius gifuensis Ashmaed) belong to Hymenoptera Aphidiidae, are a kind of advantages of cigarette aphid Parasitic wasp, at present, the technology application of the anti-Tobacco Control aphid of aphidius gifuensis, prevention effect have largely been carried out in the province such as Yunnan, Guizhou, Jiangxi Significantly.
During the anti-Tobacco Control aphid technology application of aphidius gifuensis, it is a critically important job that aphidius gifuensis over wintering is bred Link.The host crop of existing over wintering generally selects tobacco seedlings, Turnip Sprouts or green vegetables seedling, a kind of host plant of long-term use, disease The original is accumulated, and causes host plant disease resistance reduction, increases cost input, is unfavorable for the development of aphidius gifuensis.To ensure cigarette aphid Cocoon honeybee over wintering is bred and is smoothed out, and need to seek to be adapted to the overwintering host plant of cigarette aphid breeding, increases overwintering host plant Diversity.Ganzhou City aphidius gifuensis cultivation base has carried out a large amount of sieves to the overwintering host plant of aphidius gifuensis host (cigarette aphid) Choosing, at present screening study goes out the overwintering host plant-rape for being adapted to cultivation cigarette aphid, rape to the over wintering of aphidius gifuensis, Cultivate, expand numerous with lot of superiority.Rape, is called pakchoi, bitter dish, category Cruciferae, brassica plant, cold-resistant, drought resisting, Anti-virus ability is strong.Flower is yellow, and cigarette aphid has taxis to yellow, has stronger freezing tolerance after cigarette aphid parasitism, while oily Dish has stronger anti-virus ability, is not susceptible to virosis, is aphidius gifuensis over wintering and the good material bred.
By literature search, have no that carrying out aphidius gifuensis over wintering using winter rape breeds identical report.
The content of the invention
In view of the shortcomings of the prior art, the present invention is intended to provide one kind carries out aphidius gifuensis over wintering using winter rape The method bred, solves numerous bee technology in the prior art and is difficult to carry out aphidius gifuensis over wintering to breed, cannot meet next year Vega puts the problem of honeybee needs.
To achieve these goals, the present invention is adopted the following technical scheme that:
It is a kind of to carry out the method that aphidius gifuensis over wintering is bred using winter rape, comprise the following steps:
Winter rape is cultivated in S1 warmhouse booths:In warmhouse booth, early October starts to be sowed in batches every 15d educates Seedling, sows 4~5 times altogether;
The small-sized fly net construction of S2:
Before rape is buddingged in the winter time, fly net is built to the winter rape in warmhouse booth, the top of the fly net is used Shading net covering, humiture in regulation and control net;
The inoculation of S3 cigarettes aphid is bred:
From the Rapeseed Field of field, 2~4 ages of collection are connect as cigarette aphid original seed not by parasitic aptery larvae aphid using putting naturally Method, being inoculated in the rape that first cultivates carries out cigarette aphid breeding;15~20d is cultivated, rape in spite of illness and cigarette aphid is rejected, by nothing In the rape that the cigarette aphid renewed vaccination of disease is cultivated to second batch;Repeat the During Detoxification 2~3 times, bred;Now greenhouse is big 10~30 DEG C of temperature of shed, humidity 50~80%, allow growth of rape while cigarette aphid amount reproduction;
S4 aphidius gifuensis are inoculated with:
When cigarette aphid is up to 2000~3000 heads/plant, is gathered from the Rapeseed Field of field and select healthy and strong, individuality than larger cigarette Aphid parasite deadlock aphid, in honeybee, aphid ratio 50~100, carries out connecing honeybee using connection is put naturally, after stiff aphid sprouts wings, allows aphidius gifuensis Naturally cigarette aphid is found parasitic;
S5 aphidius gifuensis are bred:
After aphidius gifuensis inoculation, 20~28 DEG C of temperature, humidity 70~80% in warmhouse booth are kept, into temperature during bee eclosion Degree control 20~25 DEG C, humidity 75~95%, cultivate 15~18d and form a large amount of aphidius gifuensis deadlock aphids, develop into aphidius gifuensis;
S6 expanding propagations:
2 months next years to April, the aphidius gifuensis that will be obtained in step S5, as the rear batch of aphidius gifuensis of rape inoculation, are pressed According to the method for step S4, carry out being connect in warmhouse booth honeybee again with expanding propagation, obtain being largely used to 4~June in vega bulk storage Aphidius gifuensis:In honeybee, aphid ratio 50~100, carry out connecing honeybee using connection is put naturally, allow aphidius gifuensis parasitism cigarette aphid.
It should be noted that partially precocious rape variety in winter rape selection.
It should be noted that in step S1, each sown area 3m2, to ensure to have enough winter rapes standby.
It should be noted that in the step S2, the fly net net on dismountable stainless steel steelframe, sunshade net It is covered in the top of the stainless steel steelframe;The a length of 1.0m of stainless steel steelframe, a width of 3.0m, a height of 3.0m, and surrounding use Screw assembling is fixed;The fly net is with 60 mesh fly nets.
It should be noted that in step s3, the condition that cigarette aphid breeds is:Cigarette aphid inoculation after 10~30 DEG C of temperature of shed, Humidity 50~80%, reaches individual plant cigarette aphid 2000~3000.
It should be noted that in step S5, the also purification and rejuvenation including aphidius gifuensis:Form a large amount of aphidius gifuensis deadlock aphids Afterwards, remove in spite of illness and be redirected the parasitic stiff aphid of raw honeybee, retain the healthy and strong stiff aphid without hyperparasite performance, develop into aphidius gifuensis.
The beneficial effects of the present invention are:The present invention cultivates host plant-winter rape in warmhouse booth, builds small-sized Fly net, and make full use of cigarette aphid to the taxis of yellow rape flower, breed the parasitic aphidius gifuensis of cigarette aphid and survive the winter, it can be ensured that be secondary Year aphidius gifuensis high-volume supply.The present invention solves the problems, such as that aphidius gifuensis are difficult to over wintering and breed, and realizes cigarette aphid cocoon Honeybee propagation in scale and popularization.
Brief description of the drawings
Fig. 1 is embodiments of the invention schematic flow sheet;
Fig. 2 is the structural representation of small-sized fly net.
Specific embodiment
Below with reference to accompanying drawing, the invention will be further described, it is necessary to explanation, the present embodiment is with this technology side Premised on case, detailed implementation method and specific operating process is given, but protection scope of the present invention is not limited to this reality Apply example.
As shown in figure 1, a kind of specific method step that the method that aphidius gifuensis over wintering is bred is carried out using winter rape It is rapid as follows:
Host plant-winter rape is cultivated in S1 warmhouse booths:
In warmhouse booth, early October starts to be sowed in batches every 15d, sows 4~5 times altogether, each sown area 3m2, to ensure to have enough host plant-rapes standby.
The small-sized fly net construction of S2:
Before rape is buddingged (early December), detachable small-sized fly net is built to rape in warmhouse booth.Such as Fig. 2 institutes Show, a length of 1.0m of dismountable stainless steel steelframe 1, a width of 3.0m, a height of 3.0m, surrounding is assembled with screw 2 and fixed, then with 60 Purpose fly net 4 is caught, and prevents wasp fly etc. from endangering;The top sunshade net 3 of stainless steel steelframe is covered, warm and humid in regulation and control net Degree.
The inoculation of S3 cigarettes aphid is bred:
From the Rapeseed Field of field, 2~4 ages of collection are connect as cigarette aphid original seed not by parasitic aptery larvae aphid using putting naturally Method, being inoculated in the rape that first cultivates carries out cigarette aphid breeding.15~20d is cultivated, rape in spite of illness and cigarette aphid is rejected, by nothing In the rape that the cigarette aphid renewed vaccination of disease is cultivated to second batch.Repeat the During Detoxification 2~3 times, bred.Now note temperature 10~30 DEG C of room canopy temperature, humidity 50~80%, allow growth of rape while cigarette aphid amount reproduction.
S4 aphidius gifuensis are inoculated with:
When cigarette aphid is up to 2000~3000 heads/plant, is gathered from the Rapeseed Field of field and select healthy and strong, individuality than larger cigarette Aphid parasite deadlock aphid, in honeybee, aphid ratio 50~100, carries out connecing honeybee using connection is put naturally, after stiff aphid sprouts wings, allows aphidius gifuensis Naturally cigarette aphid is found parasitic;
S5 aphidius gifuensis are bred:
After aphidius gifuensis inoculation, note keeping 20~28 DEG C of temperature, humidity 70~80% in warmhouse booth, into the bee eclosion phase Between 20~25 DEG C of temperature control, humidity 75~95%, cultivate 15~18d and form a large amount of aphidius gifuensis deadlock aphids.Then purified Rejuvenation, removes stiff aphid in spite of illness and is redirected the parasitic stiff aphid of raw honeybee, retains the healthy and strong stiff aphid without hyperparasite performance, develops into cigarette aphid Cocoon honeybee.
S6 expanding propagations:
In 2 months next years to April, the aphidius gifuensis in step S5 after purificating and rejuvenating for several times are used as rear batch of rape inoculation Aphidius gifuensis, to connect carry out again in honeybee method and honeybee is connect in warm canopy expand numerous according to step S4, obtaining being largely used to 4~June exists The aphidius gifuensis that vega is dissipated.
Carry out testing and achieving good result in Rvijin Jiangxi using the inventive method, as shown in table 1:
Table 1
For a person skilled in the art, technical scheme that can be according to more than and design, make various corresponding Change and deform, and all these changes and deformation should be construed as being included within the protection domain of the claims in the present invention.

Claims (6)

1. it is a kind of to carry out the method that aphidius gifuensis over wintering is bred using winter rape, it is characterised in that to comprise the following steps:
Winter rape is cultivated in S1 warmhouse booths:In warmhouse booth, early October starts every 15d seeding and seedling raisings in batches, altogether Sowing 4~5 times;
The small-sized fly net construction of S2:
Before rape is buddingged in the winter time, fly net is built to the winter rape in warmhouse booth, sunshade is used at the top of the fly net Net covering, humiture in regulation and control net;
The inoculation of S3 cigarettes aphid is bred:
From the Rapeseed Field of field, 2~4 ages of collection, using connection is put naturally, connect not by parasitic aptery larvae aphid as cigarette aphid original seed Plant carries out cigarette aphid breeding in first rape for cultivating;15~20d is cultivated, rape in spite of illness and cigarette aphid is rejected, will be disease-free In the rape that the renewed vaccination of cigarette aphid is cultivated to second batch;Repeat the During Detoxification 2~3 times, bred;Now in warmhouse booth 10~30 DEG C of temperature, humidity 50~80%, allow growth of rape while cigarette aphid amount reproduction;
S4 aphidius gifuensis are inoculated with:
When cigarette aphid is up to 2000~3000 heads/plant, is gathered from the Rapeseed Field of field and select healthy and strong, individuality than larger cigarette aphid cocoon Honeybee deadlock aphid, in honeybee, aphid ratio 50~100, carries out connecing honeybee using connection is put naturally, after stiff aphid sprouts wings, allows aphidius gifuensis nature Find cigarette aphid parasitic;
S5 aphidius gifuensis are bred:
After aphidius gifuensis inoculation, 20~28 DEG C of temperature, humidity 70~80% in warmhouse booth are kept, into temperature control during bee eclosion System 20~25 DEG C, humidity 75~95%, cultivate 15~18d and form a large amount of aphidius gifuensis deadlock aphids, develop into aphidius gifuensis;
S6 expanding propagations:
2 months next years to April, the aphidius gifuensis that will be obtained in step S5, as the rear batch of aphidius gifuensis of rape inoculation, according to step The method of rapid S4, carries out being connect in warmhouse booth again honeybee with expanding propagation, obtains being largely used to the cigarette that 4~June dissipates in vega Aphid parasite:In honeybee, aphid ratio 50~100, carry out connecing honeybee using connection is put naturally, allow aphidius gifuensis parasitism cigarette aphid.
2. it is a kind of as claimed in claim 1 to carry out the method that aphidius gifuensis over wintering is bred using winter rape, its feature It is, winter rape partially precocious rape variety in selecting.
3. it is according to claim 1 it is a kind of carry out the method that aphidius gifuensis over wintering is bred using winter rape, it is special Levy and be, in step S1, each sown area 3m2, to ensure to have enough winter rapes standby.
4. it is a kind of as claimed in claim 1 to carry out the method that aphidius gifuensis over wintering is bred using winter rape, its feature It is that in the step S2, on dismountable stainless steel steelframe, Shading net covering is in the stainless steel for the fly net net The top of steelframe;The a length of 1.0m of stainless steel steelframe, a width of 3.0m, a height of 3.0m, and surrounding assembles fixation with screw;It is described Fly net is with 60 mesh fly nets.
5. it is a kind of as claimed in claim 1 to carry out the method that aphidius gifuensis over wintering is bred using winter rape, its feature It is that in step s3, the condition that cigarette aphid breeds is:10~30 DEG C of temperature of shed, humidity 50~80% after the inoculation of cigarette aphid, reach Individual plant cigarette aphid 2000~3000.
6. it is a kind of as claimed in claim 1 to carry out the method that aphidius gifuensis over wintering is bred using winter rape, its feature It is, in step S5, the also purification and rejuvenation including aphidius gifuensis:After forming a large amount of aphidius gifuensis deadlock aphids, remove in spite of illness and weighed The parasitic stiff aphid of parasitic wasp, retains the healthy and strong stiff aphid without hyperparasite performance, develops into aphidius gifuensis.
CN201710059739.3A 2017-01-24 2017-01-24 It is a kind of to carry out the method that aphidius gifuensis over wintering is bred using winter rape Pending CN106852301A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107343441A (en) * 2017-06-27 2017-11-14 安徽科氏丛林有机农业有限公司 A kind of protecting method of rape

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GB2000007A (en) * 1977-06-27 1979-01-04 Koppert P Article and Method for Combatting Harmful Insects in Horticulture
CN103168751A (en) * 2013-04-07 2013-06-26 中国农业科学院植物保护研究所 Expanding propagation method for aphidius gifuensis ashmeads
CN103749139A (en) * 2014-01-22 2014-04-30 云南省烟草公司玉溪市公司 Biological control method of crop-rotation tobacco field myzus persicae
CN105230575A (en) * 2015-11-09 2016-01-13 云南省烟草公司玉溪市公司 Method for increasing lipaphis erysimi breeding efficiency
CN105519496A (en) * 2016-02-16 2016-04-27 弥渡格瑞生物科技有限责任公司 Natural diffusion myzus persicae vaccination method

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2000007A (en) * 1977-06-27 1979-01-04 Koppert P Article and Method for Combatting Harmful Insects in Horticulture
CN103168751A (en) * 2013-04-07 2013-06-26 中国农业科学院植物保护研究所 Expanding propagation method for aphidius gifuensis ashmeads
CN103749139A (en) * 2014-01-22 2014-04-30 云南省烟草公司玉溪市公司 Biological control method of crop-rotation tobacco field myzus persicae
CN105230575A (en) * 2015-11-09 2016-01-13 云南省烟草公司玉溪市公司 Method for increasing lipaphis erysimi breeding efficiency
CN105519496A (en) * 2016-02-16 2016-04-27 弥渡格瑞生物科技有限责任公司 Natural diffusion myzus persicae vaccination method

Non-Patent Citations (1)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107343441A (en) * 2017-06-27 2017-11-14 安徽科氏丛林有机农业有限公司 A kind of protecting method of rape

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Application publication date: 20170616