CN103168751B - Expanding propagation method for aphidius gifuensis ashmeads - Google Patents
Expanding propagation method for aphidius gifuensis ashmeads Download PDFInfo
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Abstract
The invention discloses an expanding propagation method for aphidius gifuensis ashmeads. The method comprises the following steps: inoculating aphidius gifuensis ashmead females which are about to spawn on rape seedlings with myzus persicaes, conducting aphidius gifuensis ashmead cultivation, collecting the aphidius gifuensis ashmeads, and achieving expanding propagation. According to the expanding propagation method for the aphidius gifuensis ashmeads, myzus persicaes serve as hosts of aphidius gifuensis ashmead propagation, optimized aphidius gifuensis ashmead inoculating ratio, optimized inoculating opportunity, aphidius gifuensis ashmead collecting are involved, a parasitic wasp expanding propagation technology of being high-efficiency, economical and easy and convenient to apply is formed through repeated exploration and improvement, and therefore the purposes of biological preventing and treating injurious insects are achieved.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of method expanding numerous aphidius gifuensis, particularly a kind of with the method for cigarette aphid numerous aphidius gifuensis for host expands.
Background technology
Aphid is one of destructive insect of most on the earth.The aphid had been found that at present always has 10 sections about 4400 kinds, and wherein nearly 250 kinds is endanger serious insect for agriculture and forestry and horticulture.Often be clustered in the positions such as blade, tender stem, bud, terminal bud, food water is sucked by thorn, make leaf-shrinkage, curling, lopsided, cause the mechanical damage of plant nutrition loss and organ, in addition, aphid can propagate viral diseases of plants, and the honeydew of its secretion can grow the causal organisms such as various plants fungus and bacterium, causes the Occurrence & epidemic of plant disease.Aphid also has extremely strong instinct of reproduction, the aphid of some kinds has unique and complex modes of reproduction, i.e. parthenogenesis, and such reproduction cycle is continued until whole summer, environmental condition just can breed for 1 generation in suitable every 5 days, within about 20-40 days, can breed many generations.Therefore a female worm can produce hundreds of millions of aphids in spring after hatching.Such as, it is female that cabbage aphid (Brevicoryne brassicae) can breed for 41 generations, if all survived, can reach 1.5 × 10
27individual offspring.
Because aphid is for the harm of plant particularly economic crops, people spend a large amount of manpower and materials to attempt to control causing harm of aphid.Wherein effective measures are a lot, such as, use the chemical insecticide of internal-suction type, just can kill aphid fast.In addition, occurring in nature has a large amount of Natural Enemies of Insects predations or parasitizes aphid, also has some bacteriums, fungi and virus infection aphid.The natural enemy insect kind of aphid is a lot, has ladybug, syrphus fly, aphid parasite, food aphid cecidomyiia, aphis lion, Chrysopa and spider etc.Wherein aphid parasite is the parasitic enemy insect of the special parasitic aphid of a class, and very remarkable to the natural contral effect of aphid, it plays irreplaceable important function in agriculture and forestry are produced.Utilize aphid parasite to prevent eliminating aphis and be conducive to reducing the use of chemical pesticide, protection of the environment, maintain the ecological balance and protection bio-diversity, significant to agricultural sustainable development implementation.
Aphidius gifuensis Aphidius gifuensis is strong with its fecundity, and natural parasitic rate is high, and the generation cycle is short, and strong adaptability is also easy to the merits such as artificial propagation and becomes one of natural enemy insect that in Aphidiidae, value is very high.This honeybee is mainly distributed in China's Mainland, Taiwan, Korea S, Japan waits east Asia area, stronger to the natural contral power of host aphid, its host range is extensive, can parasitic cigarette aphid, radish aphid, little English grain aphid, soybean aphid, cotten aphid and eggplant without net aphid etc., especially remarkable to the control efficiency of cigarette aphid.At vega, the parasitic rate of aphidius gifuensis to cigarette aphid is generally 20% ~ 60%, Gao Zheke and reaches 89.16%.In recent years, aphidius gifuensis receives both domestic and external showing great attention to, and it expands numerous obtaining with application and carries out widely, and achieves fabulous control effect in actual applications.
In view of the excellent biological and ecological methods to prevent plant disease, pests, and erosion ability of aphidius gifuensis, sum up the efficient multiplication technique of screening, a large amount of production natural enemy insect product, is significant for the aphid class pest in prevention and control China vegetables, gardening, agricultural, forestry.In recent years, we are in conjunction with aphidius gifuensis auxology, alimentology, physiology and to insect pest controlling merit rating, carry out the vast propagation technological invention research of aphidius gifuensis, around numerous honeybee host screening, best inoculation proportion, connect honeybee crucial opportunity, become the sport technique segments such as honeybee collection storage, through repeatedly exploring and improving, define efficient, economic, easy parasitic wasp multiplication technique, improve parasitic wasp output, the biocontrol efficacy facilitating aphid promotes.
In recent years, see the units such as Yunnan Academy of Tobacco Agricultural Science, Yunan Tobacco Company Yuxi Branch, Qujing City Co., Tobacco Co., Yunnan Prov., Yunnan Tobacco Co., Ltd., Dali Branch, Honghezhou Branch, Yunnan Tobacco Co., Ltd., Yunnan Provine Tobacco Science Inst. and expand numerous relevant patent of invention and utility model patent about aphidius gifuensis.Above-mentioned unit mainly adopts in small-sized temperature canopy, first plants tobacco, accesses cigarette aphid again, accesses aphidius gifuensis again, and reclaim the multiplication technique route of aphidius gifuensis adult.Such invention is mainly applicable to utilize tobacco in warm canopy, to expand numerous parasitic wasp for vector plant at south China (particularly Yunnan Province).
In the practice of expanding numerous aphidius gifuensis, we find that above-mentioned technology is unsuitable for the northern area of China, and main manifestations is: the first, the tobacco growing cycle is long, connecing aphid and often need more than the 90d time, needing a large amount of human and material resources from being seeded into; The second, tobacco growing needs larger space, and individual plant tobacco can grow to 2 meters high, needs the spacing of 1*1 rice to arrange tobacco cultivation, needs the numerous place of the expansion of larger area; 3rd, tobacco leaf has viscosity, is unfavorable for that parasitic wasp is collected; 4th, be gathered into honeybee, be unfavorable for product storage and transport.
In sum, be badly in need of one at present and can overcome above shortcoming, be suitable for the technology expanding numerous aphidius gifuensis in the northern area of China, thus meet the needs of cigarette aphid biological control in our northern agricultural production.
Summary of the invention
The object of this invention is to provide a kind of method expanding numerous aphidius gifuensis.
The method of the numerous aphidius gifuensis of expansion provided by the present invention, specifically can comprise the steps: to inoculate aphidius gifuensis queen bee to be laid eggs to the Brassica Napus Seedling with cigarette aphid, carries out honeybee and cultivates, collect aphidius gifuensis, realize expansion numerous.
In the process, described in the inoculation quantity of aphidius gifuensis queen bee to be laid eggs and the ratio of described cigarette aphid quantity be 1:130 to 1:190, as 1:130 to 1:160, or 1:160 to 1:190.
In one embodiment of the invention, the inoculation quantity of aphidius gifuensis queen bee to be laid eggs described in and the ratio of described cigarette aphid quantity are specially 1:130,1:160 or 1:190.
In the above-mentioned methods, the condition of culture carrying out honeybee cultivation described in is: daytime (6:00 to 20:00) temperature 25 ± 1 DEG C, night (20:00 is to 6:00 next day) temperature 20 ± 1 DEG C, relative moisture RH65% ~ 70%, photoperiod L: D=14h: 10h.
In the above-mentioned methods, the time was 4-14 days, as 4-8 days or 10-14 days.
Concrete, in the process, when described collection aphidius gifuensis is for collecting the stiff aphid of described aphidius gifuensis, described in carry out the incubation time that honeybee cultivates and can be 4-8 days (as 5 days); When described collection aphidius gifuensis becomes honeybee for the described aphidius gifuensis of collection, described carry out incubation time that honeybee cultivates can be 10-14 days (as 11 days) in certain temperature range, aphidius gifuensis is along with rising shortening growth cycle of temperature, and therefore, the breeding cycle also can correspondingly shorten.
In the above-mentioned methods, the described Brassica Napus Seedling with cigarette aphid meets following condition: with cigarette aphid as described in 3700-4200 head (concrete as 3704 or 4120) on Brassica Napus Seedling described in every strain.
In the present invention, the preparation method of the described plant seedlings with cigarette aphid comprises the steps: that inoculating female cigarette aphid to be laid eggs to described Brassica Napus Seedling becomes aphid, inoculum density is inoculated female cigarette aphid to be laid eggs as described in 50-70 head (as 50) for Brassica Napus Seedling described in every strain and is become aphid, cultivate (as cultivated 7d) with 3700-4200 head cigarette aphid (the cigarette aphid quantity on the blade newly grown in the training period is disregarded) to Brassica Napus Seedling as described in every strain, with the Brassica Napus Seedling of cigarette aphid described in obtaining.
In described method, described in inoculation when the female cigarette aphid laid eggs becomes aphid, described Brassica Napus Seedling is the Brassica Napus Seedling of 7-8 sheet true leaf (as 7 true leaves).Now, described Brassica Napus Seedling is specially growth 27-30, as grown the Brassica Napus Seedling of 27 days; The described growth Brassica Napus Seedling of 27-30 days is that rape seed after planting cultivates the Brassica Napus Seedling obtained for 27-30 days, planting seed be denoted as the 1st day day.
In said method, the described growth Brassica Napus Seedling of 27 days is according to the method preparation comprising following a and b step:
A, by the planting seed of rape, cultivate to height of seedling be 10cm;
The time of described cultivation is specially 14 days, is denoted as the 1st day sowing day;
B, step a gained Brassica Napus Seedling is transplanted in the nutritive cube containing culture matrix, continues to cultivate to the Brassica Napus Seedling obtained with 7-8 sheet true leaf;
Be specially in the above-mentioned nutritive cube (12cm × 12cm) step a gained Brassica Napus Seedling is transplanted to containing culture matrix and transplant according to the described Brassica Napus Seedling of 2, every alms bowl,
Above-mentioned culture matrix is specially the mixture that the peat composed of rotten mosses and vermiculite mix with volume ratio 2 ︰ 1;
The described time of continuing to cultivate is specially 13 days
The condition of various described cultivation is all specially day temperature 28 DEG C ± 1 DEG C, night 22 ± 1 DEG C, relative moisture 65%-70%, photoperiod L: D=14h: 10h.
In the process, described collection aphidius gifuensis specifically can be collects the stiff aphid of described aphidius gifuensis, also can be the described aphidius gifuensis of collection and becomes honeybee.
When collecting described aphidius gifuensis deadlock aphid, carry out according to the operation of following A; When collecting described aphidius gifuensis and becoming honeybee, carry out according to the operation of following B:
A by through described in go out honeybee cultivate after the Brassica Napus Seedling blade with stiff aphid in water, soak 10-15 minute, and shake the described stiff aphid that comes off simultaneously, collect described stiff aphid;
B is placed in through the Brassica Napus Seedling blade with stiff aphid gone out after honeybee cultivation the container closed openend by described, and described container is placed in collection beehive, light is provided above described collection beehive, after the stiff aphid of described aphidius gifuensis sprouts wings, open the opening of described container, utilize described aphidius gifuensis to become the phototaxis of honeybee, described aphidius gifuensis become honeybee automatically enter collection beehive complete collection.
In the above-mentioned methods, described rape is specially green bright and beautiful No. one of rape variety.
The method of the numerous aphidius gifuensis of expansion provided by the present invention, convenient, economical, be easy to normalizing operation, numerous aphidius gifuensis can be expanded by rapid, high volume, reach the object that insect biology is prevented.Advantage of the present invention is: (1) is with low cost: the seeding propagation link that technology adopts, and rape is general types, and expanding numerous matrix (vermiculite) can repeatedly use, only need through solar exposure.Nutritive cube, gauze etc. that each link adopts are all low value product, easily buy and can process voluntarily.(2) space availability ratio is high: this technology is not limit and expanded numerous space, greatly to expanding the production line of numerous natural enemy, little extremely easy greenhouse and plastic greenhouse, all can be used to expand numerous aphidius gifuensis natural enemy product.(3) batch production operation is convenient to: this technology relates to the links expanding numerous aphidius gifuensis, all intensively can operate, is convenient to factorial praluction.(4) stiff aphid and become honeybee can automatically collect, and can respectively for prevention and control object difference and collect stiff aphid, become honeybee, discharge fast, save a large amount of artificial material resources.
Embodiment
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
In following embodiment, photoperiod Lx:Dy represents light irradiation time x hour every day, dark duration y hour, x+y=24.
Cigarette aphid (Myzus persicae): be recorded in " once treasure. the general introduction of China cigarette aphid study on prevention. Agriculture of Anhui science, 2011,39 (2): 826-827. " in a literary composition, the public can obtain from Plant Protection institute, Chinese Academy of Agricultral Sciences.Environmental concerns 3d can breed for 1 generation.
Aphidius gifuensis (Aphidius gifuensis): be recorded in " Bi Zhangbao; Ji Zhengrui. aphidius gifuensis Aphidius gifuensis Ashmead biological study. Jouranl of Agricultural University of Hebei; 1993; 16 (2): 1-8. " in a literary composition, the public can obtain from Plant Protection institute, Chinese Academy of Agricultral Sciences.
For the demand that satisfied expansion is numerous, need set up the conservation population of cigarette aphid and aphidius gifuensis, the conservation environmental condition that this two population is suitable for is: day temperature 25 ± 1 DEG C, nocturnal temperature 20 ± 1 DEG C, relative moisture RH65% ~ 70%, photoperiod L: D=14h: 10h.Regularly carry out rejuvenation from field acquisition original inhabitants population.
Green bright and beautiful No. one of rape variety: can buy from Beijing middle peasant hundred standing grain development in science and technology Co., Ltd.
Embodiment 1, cigarette aphid is utilized to expand the method for numerous aphidius gifuensis
One, the cultivation of seedling
1, seedling Fruit variety
Through screening, determine to expand numerous the most suitable with rape.Kind is green bright and beautiful No. one.Select color just, particle large, full, regular shape, homogeneous seed.
2, seedling is numerous soon
Quick propagating and cultivating condition: daytime (6:00 to 20:00) temperature 28 ± 1 DEG C, night (20:00 is to 6:00 next day) temperature 22 ± 1 DEG C, relative moisture 65% ~ 70%, photoperiod L: D=14h: 10h.
1) vernalization sowing: indoor at the seedling fostering of closed isolation, composite soil (peat composed of rotten mosses and vermiculite mix with volume ratio 2 ︰ 1) is put in 72 hole seedling culture hole plates, be about 3/4 of every pore volume, then in seedling culture hole plate, be sprinkled into rape seed (about 4 ~ 5, every hole seed) and on seed, cover one deck Nutrition Soil (being advisable just not have rape seed), seedling culture hole plate is put into pallet and pours into suitable quantity of water by wetting for Nutrition Soil even (must guard against water can not be too much, prevents seed decay).
2) and time-division alms bowl: seed germination and height of seedling is that after about 10cm (sowing day is denoted as the 1st day by about 14d), Brassica campestris L seedling being moved on to diameter is in the nutritive cube of 9cm, with composite soil (peat composed of rotten mosses and vermiculite mix with volume ratio 2 ︰ 1) cultivation, 2, every alms bowl.
3) fast numerous guarantee: Brassica campestris L seedling can grow to 7 ~ 8 true leaves in 13d after point alms bowl.
4) leafy carrier connects worm: when Brassica campestris L seedling grows to 7 ~ 8 true leaves, namely can be used for next link.
Two, the expansion of host is numerous
1, host selects:
Select host---the cigarette aphid of aphidius gifuensis advantage parasitism.
2, conservation maintains: i.e. the foundation of conservation population and maintenance.
For the demand that satisfied expansion is numerous, need set up the conservation population of cigarette aphid, conservation environmental condition is: daytime (6:00 to 20:00) temperature 25 ± 1 DEG C, night (20:00 is to 6:00 next day) temperature 20 ± 1 DEG C, relative moisture RH65% ~ 70%, photoperiod L: D=14h: 10h.Regularly carry out rejuvenation from field acquisition original inhabitants population.
3, inoculate
1) selection of inoculation time
Test material: rape (a green bright and beautiful kind), cigarette aphid;
Test method: in the climatic cabinate (south of the River, Ningbo, RXZ-500C type) interior daytime (6:00 to 20:00) temperature 25 ± 1 DEG C, night (20:00 is to 6:00 next day) temperature 20 ± 1 DEG C, relative moisture RH65% ~ 70%, under photoperiod L: D=14h: 10h condition, cultivate the cigarette aphid of rape and later stage access.After rape true leaf fully launches, the true number of blade is selected to be that the Brassica Napus Seedling of the different phase of 2-10 is tested, every strain rape access aphid 50, again through 7 days continuous culture, by the aphid amount on counter random measurement 1 true leaf, estimation aphid total amount, and calculate single leaf and carry aphid amount.On the blade newly grown during this period, Aphed population is disregarded.Repeat 20 times.
Result of the test: as shown in table 1, along with the true number of blade of Brassica Napus Seedling increases, it is in rising trend that complete stool rape carries aphid total amount, and aphid amount raises trend significantly in advance, and stage keeps stable.Carry in aphid amount at mean univalent functions, fluctuating range is less, fluctuates between 410-530 head/leaf.Consider production cost, raise the efficiency, can adopt when 7-8 sheet true leaf fully launches on producing, access aphid, through 7d continuous culture, aphid total amount can reach 3700-4200 head, namely maintains 4000 heads/plant of rape levels, single leaf carries aphid amount and maintain about 500, more satisfactory.
Table 1 connects aphid time test result
2) selection of host's amount is connect
Test material: rape (a green bright and beautiful kind), cigarette aphid;
Test method: in the climatic cabinate (south of the River, Ningbo, RXZ-500C type) interior daytime (6:00 to 20:00) temperature 25 ± 1 DEG C, night (20:00 is to 6:00 next day) temperature 20 ± 1 DEG C, relative moisture RH65% ~ 70%, under photoperiod L: D=14h: 10h condition, cultivate the cigarette aphid of rape and later stage access.After rape the 7th true leaf fully launches, every strain rape access aphid, connect borer population amount be respectively every strain 10,30,50,70,90,110,150, then through 7 days continuous culture, calculate and always carry aphid amount.By the aphid amount on counter random measurement 1 true leaf, estimation aphid total amount, calculates single leaf and carries aphid amount, and observe aphid build.On the blade newly grown during this period, Aphed population is disregarded.Repeat 20 times.
Result of the test: connect aphid quantity Choice tests result as shown in table 2, therefrom visible, when to connect aphid amount be 50 heads/plant, after 7d continuous culture, aphid sum reaches 3980, and single leaf carries aphid amount 568.9; When connecing aphid amount and being 70 heads/plant, aphid sum reaches 4000, and single leaf carries aphid amount and is reached for 571.7, but aphid individuality diminishes, and aphid parasite has not been liked parasitism and laid eggs; Connect aphid amount when being elevated to 90 heads/plant further, aphid sum reaches 4200, and single leaf carries aphid amount and increases to 600.Connect aphid amount when raising again, aphid sum maintains the scale of 4200 heads/plant substantially, single leaf carries worm amount and then maintains higher level, except aphid build significantly diminishes, have also appeared a large amount of alatae, present aphid Population excessive, the nutrition supply of rape host is not enough, alatae borer population amount increases, and flight phenomenon of migrating occurs.Consider production cost, expand the factors such as numerous efficiency, production is determined with the borer population amount that connects of 50 heads/plant be good, also can suitably increase inoculation quantity, be advisable with 50-70 head/plant.
Table 2 connects aphid quantity Selection experiment result
4, batch expands numerous
At interval of within 2 weeks, cultivating seedling as stated above, saturatedly connecing worm, produce with the batch that it realizes relay, meet prevention and control requirement, ensure Product supply.
Three, numerous honeybee
1, the suitableeest determination connecing honeybee ratio
Test material: rape (a green bright and beautiful kind), cigarette aphid and aphidius gifuensis;
Test method: in the climatic cabinate (south of the River, Ningbo, RXZ-500C type) interior daytime (6:00 to 20:00) temperature 25 ± 1 DEG C, night (20:00 is to 6:00 next day) temperature 20 ± 1 DEG C, relative moisture RH65% ~ 70%, under photoperiod L: D=14h: 10h condition, cultivate cigarette aphid and the aphidius gifuensis of rape and later stage access.After rape the 7th true leaf fully launches, every strain rape access aphid 50, again through 7 days continuous culture, now aphis population quantity reaches 4000 heads/plant, the aphidius gifuensis queen bee just sprouted wings is placed in mixed population 1d, after completing mating, again in 1:100,1:130,1:160,1:190,1:220,1:250 honeybee aphid ratio access aphidius gifuensis queen bee (completed mating, stalwartness and wait to lay eggs), namely respectively every strain Brassica campestris L seedling access 40,31,25,21,18,16 aphidius gifuensis queen bees (completed mating, stalwartness and wait to lay eggs).The stiff aphid quantity of aphidius gifuensis is calculated after cultivating 5d.By the stiff aphid amount on counter random measurement 1 true leaf, estimation total amount, and calculate single honeybee contribution rate (single honeybee contribution rate=stiff aphid sum/connect honeybee number).On the blade newly grown during this period, stiff aphid number is disregarded.Repeat 20 times.
Result of the test: connect honeybee scale experimental result as table 3, therefrom the visible increase with connecing honeybee quantity, aphidius gifuensis stiff aphid quantity is in rising trend and tend to be steady gradually, and single honeybee contribution rate is then the downward trend again that first rises.This is mainly because aphidius gifuensis is in the behavior of parasitic aphid, there is terebra and sound out process, generally to select aphid terebra through repeated multiple times, after judging that this aphid meets filial generation nutritional need, just lay eggs in aphid body, if aphidius gifuensis density is too high, to aphid, terebra is frequent repeatedly, the mechanical damage formed will cause more cigarette aphid dead, and the aphidius gifuensis of having laid eggs in this aphid body can not complete growth course, have impact on the formation of stiff aphid.From stiff aphid quantitative index, honeybee aphid ratio is between 1:100-160, and stiff aphid quantity maintains the level of 900 heads/plant, reaches higher level; From the angle of single honeybee contribution rate, honeybee aphid ratio is the interval of 1:160-250, and single honeybee contribution rate maintains the higher level of 35 stiff aphid/honeybees.Comprehensive production is actual, when honeybee aphid ratio is 1:160, has both played the parasite efficiency of parasitic wasp the best, and the stiff aphid quantity of formation is also maximum, is Utopian inoculation proportion.
Table 3 best inoculation proportion screening test result
The result of comprehensive above each test, expanding numerous aphidius gifuensis best of breed is: vegetative period is that Brassica Napus Seedling (7-8 sheet true leaf)+every strain rape of 27d is inoculated 50 female cigarette aphids to be laid eggs and become aphid to cultivate 7d+ to connect honeybee and become honeybee than the female aphidius gifuensis to be laid eggs for 1:160.This combination can save numerous honeybee cost greatly, can promote large-scale breeding and even the merchandized handling of aphidius gifuensis.
2, the determination of breeding cycle
When indoor propagation aphidius gifuensis, from plantation host plant rape, inoculation host cigarette aphid, inoculation aphidius gifuensis to one-tenth bee eclosion, need about 44d altogether.From inoculation aphidius gifuensis to gathering in the crops into honeybee, whole numerous honeybee cycle is approximately 11d, refers to table 4.In certain temperature range, aphidius gifuensis is along with rising shortening growth cycle of temperature, and therefore, the breeding cycle also can correspondingly shorten.
The table 4 aphidius gifuensis production cycle
| Production procedure | Time (my god) |
| Cultivate Brassica campestris L seedling | 1～27 |
| Inoculation cigarette aphid | 28 |
| The Brassica campestris L seedling of cigarette aphid has been inoculated in management | 29-33 |
| Inoculation aphidius gifuensis | 34 |
| The growth of aphidius gifuensis | 35-43 |
| Collect aphidius gifuensis and become honeybee | 44 |
Four, automatically collecting
Product-collecting technology can be divided into two classes by the difference of collecting worm state: a class is for collecting stiff aphid, and a class is gathered into honeybee, takes different collection methods respectively.Short owing to being gathered into the honeybee survival life-span, be unfavorable for the storage of natural enemy insect product, packaging, long distance transportation, be also not easy to field adaptability, carry out the method for collecting so invention also provides for stiff aphid.
The method of collecting stiff aphid (carrying out for 4-8 days after connecing honeybee) is comparatively simple, is also convenient to make pupa card.Method collects semiarid rape leaf, and insert in the tank of covering gauze, inundation 10 ~ 15 minutes, period shakes gently, and the stiff aphid of aphidius gifuensis can come off from blade, and floats on upper strata, uses bamboo strainer to pull out.Collect the stiff visual market of aphid of gained to the required time of aphidius gifuensis product, take the circumstances into consideration to adjust temperature, to accelerate or delayed development progress, control the time that it turns into aphidius gifuensis.
As collected adult, aphidius gifuensis adult can be utilized to have assemble upward, the habit of the light that becomes, insert in small-sized cage by the stiff aphid sprouted wings soon together with rape leaf, cage is inverted funnel-form, and enclose with lighttight black cloth, opening is left in top.Before collecting, opening is tightened, insert in collection beehive, and in overhung incandescent lamp one.After a large amount of adult eclosion, can open incandescent lamp, utilize aphidius gifuensis to become the phototaxis of honeybee, make it to be gathered in opening part, untie the opening tightened in good time, aphidius gifuensis becomes honeybee automatically to enter collection beehive.
Claims (8)
1. expand a method for numerous aphidius gifuensis, comprise the steps: to inoculate aphidius gifuensis queen bee to be laid eggs to the Brassica Napus Seedling with cigarette aphid, carry out honeybee and cultivate, collect aphidius gifuensis, realize expansion numerous;
The described Brassica Napus Seedling with cigarette aphid meets following condition: with 3700-4200 described cigarette aphid on Brassica Napus Seedling described in every strain;
The inoculation quantity of aphidius gifuensis queen bee described to be laid eggs and the ratio of described cigarette aphid quantity are 1:130 to 1:190;
Expanding numerous condition is: daytime 6:00-20:00 temperature 25 ± 1 DEG C, 20:00-at night 6:00 next day temperature 20 ± 1 DEG C, relative moisture RH65% ~ 70%, photoperiod L: D=14h: 10h.
2. method according to claim 1, is characterized in that: in described method, described in carry out the incubation time that honeybee cultivates be 4-14 days.
3. method according to claim 1, it is characterized in that: in described method, the described Brassica Napus Seedling with cigarette aphid obtains according to the method comprised the steps: inoculate female cigarette aphid to be laid eggs to described Brassica Napus Seedling and become aphid, inoculum density is inoculated female cigarette aphid to be laid eggs described in 50-70 head for Brassica Napus Seedling described in every strain and is become aphid, be cultured to 3700-4200 head cigarette aphid on Brassica Napus Seedling described in every strain, with the Brassica Napus Seedling of cigarette aphid described in obtaining.
4. method according to claim 3, is characterized in that: in described method, and described in inoculation when the female cigarette aphid laid eggs becomes aphid, described Brassica Napus Seedling is the Brassica Napus Seedling of 7-8 sheet true leaf.
5. method according to claim 3, is characterized in that: in described method, and described in inoculation when the female cigarette aphid laid eggs becomes aphid, described Brassica Napus Seedling is the growth Brassica Napus Seedling of 27-30 days; The described growth Brassica Napus Seedling of 27-30 days is that rape seed after planting cultivates the Brassica Napus Seedling obtained for 27-30 days, planting seed be denoted as the 1st day day.
6. method according to claim 1, is characterized in that: in described method, and described collection aphidius gifuensis is for collecting the stiff aphid of aphidius gifuensis, or collection aphidius gifuensis becomes honeybee.
7. method according to claim 6, is characterized in that: the stiff aphid of the described aphidius gifuensis of described collection is following A; The described aphidius gifuensis of described collection becomes honeybee to be following B:
A by through described in go out honeybee cultivate after the Brassica Napus Seedling blade with stiff aphid in water, soak 10-15 minute, and shake the described stiff aphid that comes off simultaneously, collect described stiff aphid;
B is placed in through the Brassica Napus Seedling blade with stiff aphid gone out after honeybee cultivation the container closed openend by described, and described container is placed in collection beehive, light is provided above described collection beehive, after the stiff aphid of described aphidius gifuensis sprouts wings, open the opening of described container, utilize described aphidius gifuensis to become the phototaxis of honeybee, described aphidius gifuensis become honeybee automatically enter collection beehive complete collection.
8., according to described method arbitrary in claim 1-7, it is characterized in that: described rape is green bright and beautiful No. one of rape variety.
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| CN106852301A (en) * | 2017-01-24 | 2017-06-16 | 赣州市烟草科学研究所 | It is a kind of to carry out the method that aphidius gifuensis over wintering is bred using winter rape |
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