CN105165726B - A method of expanding numerous aphidius gifuensis using cuttage plant three-dimensional - Google Patents
A method of expanding numerous aphidius gifuensis using cuttage plant three-dimensional Download PDFInfo
- Publication number
- CN105165726B CN105165726B CN201510272588.0A CN201510272588A CN105165726B CN 105165726 B CN105165726 B CN 105165726B CN 201510272588 A CN201510272588 A CN 201510272588A CN 105165726 B CN105165726 B CN 105165726B
- Authority
- CN
- China
- Prior art keywords
- aphid
- cuttage
- bee
- numerous
- aphidius gifuensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The invention discloses a kind of method for expanding numerous aphidius gifuensis using cuttage plant three-dimensional, this method includes with the next stage: 1) cultivating host plant chrysanthemum Radix Notoginseng；2) the numerous aphid of aphid is connect；3) the numerous bee of bee is connect；4) stiff aphid production bee card is collected.The present invention is short with cycle time, demand space is small, and stiff aphid is easy to the characteristics of collecting.This method combination can greatly save numerous bee cost, can promote the large-scale breeding or even merchandized handling of aphidius gifuensis.
Description
Technical field
The present invention relates to a kind of methods for expanding numerous aphidius gifuensis using cuttage plant three-dimensional, and in particular to vector plant sieve
The methods of choosing, vector plant cuttage and quick-propagation, the numerous bee of module, stiff aphid collection combination.
Background technique
Cigarette aphid [Myzus persicae (Sulzer)] belongs to Homoptera, Aphidiadae.Also known as black peach aphid, it is commonly called as aphid, aphid.Cigarette aphid
It is distributed in all over the world, each cigarette district in China north and south generally occurs.Its plasmodium cynomolgi is miscellaneous, and the host plant of cigarette aphid reaches known to China
170 kinds or more.Reported host up to 50 more than 400 kinds of sections, predominantly Solanaceae in the world, Cruciferae, composite family and pulse family
Plant.The worm removes direct piercing and sucking plant juice, causes plant moisture and trophic disturbance outside atrophy, is also passed by the approach of piercing and sucking
A variety of virosis are broadcast, dark mildew is led to due to secretion honeydew, is an important pests on tobacco.
There are many Natural Enemies of cigarette aphid, common type have aphidius gifuensis, coccinella septempunctata, harmonia axyridia, Propylaea japonica, in
Magnificent Chrysopa, chrysopa septempunctata, beautiful Chrysopa etc..Aphidius gifuensis is strong with its fecundity, and natural parasitic rate is high, and the generation cycle is short, adaptable
And be easy for workers to the merits such as breeding become one of very high natural enemy insect of utility value in Aphidiidae (Zhao Wanyuan, 1980；
Xin is also fragrant, and 1986；Fukui et al.,1988；Bi Zhangbao, 1996；Chi and Su,2006).The bee is mainly distributed on China
Continent, Taiwan, South Korea, the east Asias such as Japan area (Chi and Su, 2006；Ohta and Ohtaishi, 2006), right
The natural contral power of host aphid is stronger, and host range is extensive, can parasitic cigarette aphid, radish aphid, small grain aphid, soybean aphid,
Cotten aphid and eggplant without net aphid etc. (Takada, 1976；Xin is also fragrant, and 1986；Nakata, 1995；Yamamoto,1997；Ohta et
al.,2001；Takada, 2002；Ohta and Ohtaishi, 2004), (Ren Guangwei especially significant to the control efficiency of cigarette aphid
Deng 2000).In tobacco field, aphidius gifuensis is usually 20%-60% to the parasitic rate of cigarette aphid, and high person is up to 89.16% (Chen Jiahua
Deng 1990；He Zhonglin, 1986).In recent years, aphidius gifuensis receives highest attention both domestic and external, expands numerous and application and obtains
It is extensive to carry out, and fabulous control effect is achieved in practical applications.In view of the excellent biological and ecological methods to prevent plant disease, pests, and erosion ability of the parasitic wasp, summarize
Screen efficient multiplication technique, mass production natural enemy insect product, for the aphid class in prevention and control China vegetables, gardening, agricultural, forestry
Pest is of great significance.
Expanded numerous aphidius gifuensis in the past, often using the side for first planting tobacco, accessing aphid again, then accessing and expand numerous natural enemy
Method.In recent years, Yunnan Academy of Tobacco Agricultural Science, Yunan Tobacco Company Yuxi Branch, Yunnan Province tobacco public affairs are seen
Take charge of Qujing company, Yunnan Tobacco Co., Ltd., Dali Branch, Honghezhou Branch, Yunnan Tobacco Co., Ltd., Yunnan Province tobacco science
The units such as research institute expand numerous relevant patent of invention and utility model patent about aphidius gifuensis.
Above-mentioned unit mainly uses in small-sized temperature canopy, first plants tobacco, accesses cigarette aphid again, accesses aphidius gifuensis again, and
Recycle the multiplication technique route of aphidius gifuensis adult.Such invention is primarily adapted for use in (especially Yunnan Province) utilization in south China
Tobacco is that vector plant expands numerous parasitic wasp in warm canopy.
In the practice for expanding numerous aphidius gifuensis, it has been found that above-mentioned technology has certain limitation, is mainly shown as: the
One, the tobacco growing period is long, connects aphid from being seeded into and generally requires 90d or more the time, needs a large amount of human and material resources；The
Two, tobacco growing needs larger space, and single plant tobacco can grow to 2 meters of height, needs 1 × 1 meter of spacing to arrange tobacco cultivation, needs
Want the numerous place of the expansion of larger area；Third, tobacco leaf have viscosity, are unfavorable for parasitic wasp collection；4th, the patent collect at
Bee is unfavorable for product storage and transport.
Summary of the invention
Aiming at the problems existing in the prior art, expand numerous aphidius gifuensis using cuttage plant three-dimensional the present invention provides a kind of
Method, choose chrysanthemum Radix Notoginseng as host plant, and by cottage method cultivation, carry out connecing the numerous aphid of aphid on chrysanthemum Radix Notoginseng, connect bee
Numerous bee collects stiff aphid production bee card, has that cycle time is short, demand space is small, and stiff aphid is easy to the characteristics of collecting.This method combination
Numerous bee cost can be greatlyd save, the large-scale breeding or even merchandized handling of aphidius gifuensis can be promoted.
The purpose of the present invention is achieved through the following technical solutions:
A method of expanding numerous aphidius gifuensis using cuttage plant three-dimensional, this method includes with the next stage:
1) host plant chrysanthemum Radix Notoginseng is cultivated；
2) the numerous aphid of aphid is connect；
3) the numerous bee of bee is connect；
4) stiff aphid production bee card is collected.
Further, further include the stage in the method: before described 1) stage, establishing cigarette aphid and aphidius gifuensis
Conservation population, and periodically rejuvenation is carried out from field acquisition original inhabitants population.
Further, the conservation environmental condition that the cigarette aphid and aphidius gifuensis population are suitable for are as follows: 25 ± 1 DEG C of day temperature, night
Between 20 ± 1 DEG C of temperature, relative humidity RH65%-70%, photoperiod L: D=14h: 10h, intensity of illumination 5000Lx.
Further, described the 1) in the stage, cultivates chrysanthemum Radix Notoginseng using cottage method, indoors throughout the year can cuttage.
Further, the time of the cuttage between December to the March of second year of current year by carrying out.
Further, described the 1), 2), 3), 4) in the stage, expand numerous frame using solid, be divided into several layers nursery inside frame body
Bed, nursery bed depth 20cm, every layer of seedling bed overhung LED light.
Further, the seedling medium that the seedling bed uses are as follows: using vermiculite, perlite, turfy soil as matrix, in seedling bed
The interior matrix for laying thickness 8-10cm, strikes off, carries out sterilization with liquor potassic permanganate.
Further, the cutting that the chrysanthemum Radix Notoginseng uses are as follows: take mature tender stem cuttage, cutting from healthy and strong chrysanthemum Radix Notoginseng mother's stem
Long 3-5 piece leaf plucks radical leaves and inserts in seedling bed, moisturizing of shading.
Further, condition is chosen in the cutting of the chrysanthemum Radix Notoginseng are as follows: cuttage selection grows 4-6 weeks branch in winter and early spring room
Item, Summer Indoor cuttage selection grow 3-5 weeks branch, and growth time is more than that the branch of 60d or more only takes the portion on its nearly top
Point；The branch lower end thickness of selection is greater than 0.3cm, and the spigot of cutting cuts flat with inclined-plane that is neat or being cut into 45 degree of angles, cutting with cutter
Length 8-10cm, upper end retains the blade of 1/4-1/3, remaining blade all cuts.
Further, the carbendazim that the cutting concentration got ready is 0.5% is first impregnated into 20-30min before the cuttage, taken out
It is rinsed well with clear water, the cuttage again after the moisture on its surface dries；First nursery bed substrate is ditched when cuttage, or with small wood
Rod pricks cave, and depth is 3-4cm, then cutting is put in, and righting is simultaneously filled out sand and pushed down, and spacing 5-7cm reserves the sky of root growth
Between；Multirow cuttage keeps line-spacing 8-10cm；Cuttage finishes water spray, makes loose matrix natural sediment, finally with transparent plastics
Membrane cover is tight, moisturizing.
Further, the numerous condition of the expansion of the cutting are as follows: temperature should keep 20-26 DEG C, and the relative humidity in bed should be kept
75%, temperature is excessively high it should be noted that ventilation, a small amount of water is sprayed every 1-2d, in the vitality and bed to keep cutting in cutting
Humidity；When the length for the root that cutting is grown reaches 5cm, water in suitable control seedling bed is reduced wet in seedling bed
Degree prevents the excessive excessive growth of root system, increases ventilation time and light application time, makes cutting gradually reform of nature environment.
Further, described the 2) in the stage, the access when chrysanthemum Radix Notoginseng of cuttage restores to grow 10 blades or 20d growth period
Aphid.
Further, monolithic blade face can negative worm 400, unit area larva number be 35/cm2。
Further, every plant of chrysanthemum Radix Notoginseng cigarette receiving aphid adult aphid 50, makes its oviposition, and after 7d, cigarette aphid quantity realizes that saturation type connects worm.
Further, described the is entered after 7d 3) stage, cigarette receiving aphid parasite continuously cultivates 25d.
Further, bee aphid ratio 1:150-200 when bee is met.
Further, sex ration when putting bee is 2: 1, daily 6000, continuous 5d；About 11d is completed at 28 DEG C of aphidius gifuensis
A generation, high temperature guarantee under nutritional condition female drone property than being about 1.2:1, and hatching rate 90%, aphidius gifuensis can expand numerous 2 generation after 25d.
Further, described the 4) in the stage, collects stiff aphid to numerous aphidius gifuensis is expanded, and make bee card.
Further, it is smeared on bee card two-sided using stiff aphid powered brush by under the aphidius gifuensis deadlock aphid brush in leaf of Radix gynurae segeti face
The stiff aphid of collection is adhered on double-sided adhesive by glue, and the bee card of 200 stiff aphids is made.
Further, it cultivates seedling respectively according to the above method at interval of 25d, connects worm, connects bee, realize the batch of anniversary relay
Formula production, meets prevention and control requirement, guarantees product supply.
It is had the advantage that using the method for expanding numerous aphidius gifuensis using cuttage plant three-dimensional of above-mentioned technical characteristic
The efficient multiplication technique of the aphidius gifuensis of this patent, it is convenient, economical, be easy to normalizing operation, can rapid, high volume expand it is numerous
Aphidius gifuensis achievees the purpose that prevent pest biology.
(1) quick and convenient: to take the mode of cuttage, dramatically reduce workload when vector plant breeding.Previous cigarette
Aphid parasite expansion is numerous will to plant tobacco, rape etc., take time and effort, the period is too long.The present invention is using vegetative manner, cuttage
Chrysanthemum Radix Notoginseng can be used to expand numerous aphidius gifuensis.
(2) low in cost: technology expands numerous link using each, and Gynura Cass frequently seen plants expand numerous matrix (vermiculite, pearl
Rock etc.) it is used multiple times, it need to be only exposed to sunlight, make the three-dimensional raw material for expanding numerous frame, easily buy and can voluntarily process.
(3) space utilization rate is high: the mode that this technology takes three-dimensional expansion numerous improves the single layer for expanding numerous aphidius gifuensis in the past
Plane formula (can only expand the plants such as numerous tobacco using ground) method, is greatly improved space utilization rate.All expand numerous link not
Limit expands numerous space can be used to expand numerous aphidius gifuensis day greatly to the production line, as low as easy greenhouse and plastic greenhouse for expanding numerous natural enemy
Enemy property product.
(4) operate convenient for batch production: this technology is related to expanding the links of numerous aphidius gifuensis, intensive can operate, just
In the factorial production.
(5) stiff aphid is collected quickly, convenient for the storage of aphidius gifuensis product, transport and release, saves a large amount of artificial material resources.
Detailed description of the invention
Fig. 1 is farrowing amount statistical chart of the cigarette aphid on different host plants；
Fig. 2 is hundred stiff aphid weight statistical charts that aphidius gifuensis is obtained on different host plants.
Specific embodiment
It is of the invention to reach the technical means and efficacy that predetermined technique purpose is taken further to illustrate, below in conjunction with
Attached drawing and preferred embodiment, it is as follows to structure of the invention, detailed description of the workflow.
Aphidius gifuensis (Aphidius gifuensis) of the present invention is strong with its fecundity, natural parasitic rate is high, generation
For the period it is short, it is adaptable and be easy for workers to breeding etc. merits become Aphidiidae in the highest natural enemy insect of utility value
One of.There is lot of advantages using this parasitic wasp control aphid, it is most important that chemical pesticide can be substituted, reach prevention and control aphid
The purpose of harm produces green agricultural product.In addition, the parasitic wasp there are also other advantages, specifically includes that (1) its annidation
By force, distributional region is wide, and each agriculture district such as China northeast, North China, northwest, Central China, south China, southwest can all survive；(2) it is applicable to
Multiple kinds of crops, fruit tree, veterinary antibiotics etc.；(3) it can the important agricultural that occur extensively of parasitic a variety of aphids, especially China
Aphid, such as cigarette aphid, green bugs, radish aphid；(4) its developmental rate is fast, and population rapidly can increase and colonize；(5)
Its pest control effect is good, can quickly and efficiently prevent causing harm for aphid；(6) its pupa and adult can be easy to use meat in field
Eye observes that insect product is easy storage, is received using easy by peasant in production.
The present invention expands numerous aphidius gifuensis, including following link: vector plant sieve using cuttage plant three-dimensional scale
The numerous bee of the fast numerous-module of choosing-vector plant-collection deadlock aphid.Technical solution is described in detail to each link below:
(1) vector plant screening technique
Chrysanthemum Radix Notoginseng [Gynura divavicata (L.) DC.] composite family Radix Notoginseng category, is called gynura divaricata, chrysanthemum Radix Notoginseng.Chrysanthemum three seventh is that
A kind of edible tender tip and tender leaf, famous-brand and high-quality special vegetable full of nutrition.Chrysanthemum Radix Notoginseng plant strain growth is vigorous, and branch power of regeneration is strong,
Resistance is strong, drought-enduring, heat-resisting, barren-resistant, pyrophilous moist climate, grows 20-30 DEG C of thermophilic, more resistance to shade.In thermophilic and sunlight
Under sufficient environment, fast growing is healthy and strong.The easily raw adventitious root in the stipes portion of chrysanthemum Radix Notoginseng, is suitable for cutting propagation, from healthy and strong female stem
Take mature tender stem cuttage, cutting long 10cm, 3-5 piece leaf plucks radical leaves and inserts in seedbed, moisturizing of shading, 10-15 days can be at
It is living, it is easy to take root with spring and autumn.
For cigarette aphid main parasitic on tobacco and crucifer, we have found that chrysanthemum Radix Notoginseng can expand numerous cigarette aphid in an experiment,
And then expand numerous aphidius gifuensis.Chrysanthemum Radix Notoginseng has this big advantage of cutting propagation, therefore predicts that it can substitute other common hosts and plant
Object such as tobacco etc. expands numerous aphidius gifuensis.The numerous effect of the influence and the expansion to aphidius gifuensis that chrysanthemum Radix Notoginseng develops cigarette aphid is all not
It appears in the newspapers.Therefore, tobacco, wheat, rape, eggplant, chrysanthemum Radix Notoginseng this 5 kinds of host plants are selected, the development of cigarette aphid on it is inquired into
With the ontogeny situation of characteristics and the aphidius gifuensis such as breeding, compare cigarette aphid survival rate and reproductive capacity on several host plants
Difference and different host plants effect numerous for the expansion of aphidius gifuensis.
Experiment 1: vector plant screening and adaptability evaluation test
1 materials and methods
1.1 material
1.1.1 test plant
Test plant is tobacco, wheat, rape, eggplant and the chrysanthemum Radix Notoginseng of Solanaceae.Seedling is cultivated in disk of nursing young plants in hothouses, to
When tobacco, rape, eggplant length to 3-4 piece true leaf, in transplanting to nutritive cube (diameter 9cm, high 10cm), using novel mixed fertilizer
Continue to cultivate, one plant of every basin.Wheat seeding is placed directly within seedlings nursing plate, no longer divides alms bowl.Chrysanthemum Radix Notoginseng is long-term cutting propagation, and interception growing way is good
Good 10cm tender stem is removed lower blade and transplanted to nutritive cube (diameter 9cm, high 10cm), continued using novel mixed fertilizer
It cultivates, it is stand-by after surviving.It places respectively and is used as test plant in dependent insect cage (40 × 40 × 50cm), plant (5-8 leaf is selected in test
Age) upper blade.
1.1.2 for trying cigarette aphid
Plant Protection institute, Chinese Academy of Agricultral Sciences, base laboratory, Langfang tobacco seedling green type cigarette aphid is picked up from, in greenhouse
After interior raising cigarette aphid number generation, the cigarette aphid of enough homogenizations is transferred on four kinds of host plants and continuously raises number generation, completely to it
After adaptation, cigarette aphid is developed to stand-by when 3-4 age.
1.1.3 for trying aphidius gifuensis
Aphidius gifuensis picks up from Plant Protection institute, Chinese Academy of Agricultral Sciences, base laboratory, Langfang tobacco seedling, for examination when
It is more than generation continuously to raise 10 using cigarette aphid as host in greenhouse.
1.2 test method
1.2.1 different host plants test the development duration of cigarette aphid and the influence of breeding
Using single head method for breeding: raising single head adult aphid with for the young leaflet tablet for trying plant seedling stage, remove adult aphid afterwards for 24 hours.It will
Vacuum side of blade is put into bottom upward and is covered in the transparent plastic culture dish (diameter 9cm, high 1cm) of wet filter paper, uses fur
1 age larvae aphid is transferred on blade by pen, 1, every ware, 30 repetitions of each host.Daily 8:00 and 20:00 respectively observes 1 time, in detail
The thin husking for recording cigarette aphid, farrowing amount, death condition, until all test worms are dead.Husking and new is rejected in observation every time
Larvae aphid is produced, every other day plus water and renews fresh leaves.
1.2.2 influence test of the different host plants to cigarette aphid reproductive speed
4 cigarette aphid adult aphids on 5 kinds of host plants are taken to connect on similar host plant respectively, for the ease of counting aphid amount,
Another 4 kinds of host plants other than wheat are removed into lobus cardiacus, only stay 3-4 piece leaf, and tight with dependent insect cage cover, observation 1 in every 2 days
It is secondary, 10d is observed continuously.Aphid number on every young plant is counted, every processing investigates 5 plants.
1.2.3 different host plants test the influence that aphidius gifuensis is developed
Aphidius gifuensis is discharged and supporting for 5 kinds of host plants is being housed by 1: 1 (female: male) pairing according to the bee aphid ratio of 1:250
In worm cage.It since occurring stiff aphid on plant, is chosen down, is packed into dactylethrae day by day.It takes and generates on different host plants
Aphidius gifuensis deadlock aphid after for 24 hours weighs hundred nose heave amounts.Continue to match at bee after emergence, connects and planted in the host for having cigarette aphid parasitism
On object, it is numerous to be placed in expansion in greenhouse.The stiff aphid that several aphidius gifuensis are acquired from the wheat in Langfang base experimental plot goes back to laboratory, makees
For control.
The calculating of 1.3 parameter and populations
With d (x) for unit interval, life table of the cigarette aphid on different host plants is constructed, according to following public affairs
Formula calculates each life table parameters:
Net breeding rate R0=∑ lxmx；
Average generation cycle T=∑ xlxmx/∑lxmx；
Intrinsic rate of increase rm=lnR0/T；
Finite rate of increase λ=erm；
Population doubling time t=ln2/rm=0.6931/rm
In formula: x is given age, d；lxFor the survival rate during x；mxIt is average per female litter size during x.
Because aphid multiplies the next generation in a manner of single-female generation, thus not design than this column.
1.4 data processing
Test data statistical analysis is all made of Excel2003 and SAS9.1 version, carries out ANOVA variance analysis, and difference is aobvious
The detection of work property uses Deng Kenshi duncan's new multiple range method.
2 results and analysis
Influence of the 2.1 different host plants to cigarette aphid development duration
The development duration of cigarette aphid on 15 kinds of host plants of table
| Test content | Chrysanthemum Radix Notoginseng | Tobacco | Rape | Eggplant | Wheat |
| 1 age nymph | 2.241±0.06a | 1.828±0.11b | 1.759±0.07b | 2.142±0.11a | 1.795±0.07b |
| 2 age nymphs | 1.893±0.05a | 1.917±0.10a | 1.854±0.05a | 2.012±0.32a | 1.864±0.05a |
| 3 age nymphs | 2.042±0.11a | 1.905±0.41a | 1.923±0.07ab | 1.711±0.05b | 1.886±0.08ab |
| 4 age nymphs | 1.750±0.08b | 1.838±0.21ab | 1.952±0.10ab | 1.824±0.06ab | 1.971±0.11a |
| The larvae aphid phase | 7.917±0.11a | 7.811±0.21b | 7.405±0.08b | 7.324±0.05b | 7.647±0.06ab |
| Reproduction early period | 1.000±0.19b | 0.7222±0.09c | 1.136±0.14b | 1.523±0.20a | 1.500±0.07a |
| Reproduction period | 13.00±0.19a | 13.091±0.12a | 9.933±0.10b | 12.801±0.22a | 13.786±0.13a |
| The reproduction later period | 1.357±0.09ab | 1.308±0.17b | 1.273±0.09b | 1.667±0.10a | 1.465±0.22a |
| Life of Adult | 15.143±0.12a | 14.308±0.13a | 14.60±0.11a | 11.60±0.10b | 13.223±0.73a |
| Generation time | 22.225±0.13b | 22.005±0.14b | 18.924±0.15c | 20.437±1.03b | 22.79±0.16a |
Note: data are average ± standard error in table；Different English lower cases after data of going together indicate that difference is aobvious
It writes (p < 0.05, Deng Kenshi duncan's new multiple range method).
As shown in Table 1, different host plants have a significant impact the development duration of cigarette aphid.On 5 kinds of host plants, 1 age
Nymphal period is considerably longer than other three kinds of hosts on chrysanthemum Radix Notoginseng (2.241d)；2 age nymphal periods differ on 5 kinds of host plants
Maximum is tobacco (1.917d) and rape (1.854d), and chrysanthemum Radix Notoginseng tends to be intermediate；3 age nymphal periods on chrysanthemum Radix Notoginseng up to
2.042d, most short on eggplant is 1.711d, differs 0.331d；Most short on 4 age nymphal period chrysanthemum Radix Notoginseng is 1.750d, and in wheat
It is comparatively long on (1.971d) and rape (1.952d)；Summarize the larvae aphid phase go through on phase chrysanthemum Radix Notoginseng longest (7.917d) with most
(7.324) differ 0.593d on short eggplant.It is larger that phase gap on 5 kinds of host plants is gone through in cigarette aphid reproduction early period, longest to be
Eggplant (1.5d), shortest is tobacco (0.722d)；Reproduction period goes through the phase in addition to most short on rape (9.933d), chrysanthemum Radix Notoginseng,
More than 13d on wheat and tobacco；The reproduction later period is 1.5d or so on 5 kinds of host plants；Life of Adult is on chrysanthemum Radix Notoginseng
Longest (15.143d), on eggplant most short (11.6d).
To sum up, generation time of the cigarette aphid on 5 kinds of host plants, most short on rape is 18.924d, eggplant about 20d, and its
Difference is not significant on his 3 kinds of host plants, is 22d or so.
Influence of the 2.2 different host plants to cigarette aphid reproductive capacity
Fig. 1 is farrowing amount statistical chart of the cigarette aphid on 5 kinds of different host plants.
Note: different English lower cases are marked in figure indicates significant difference (p < 0.05, Deng Kenshi duncan's new multiple range method).
As shown in Figure 1, influence significant difference of the different host plants to cigarette aphid farrowing amount.The single head on 5 kinds of host plants
The overall average farrowing amount of cigarette aphid is up to 63 on eggplant, is secondly 57.03 on rape, is 52.33 on chrysanthemum Radix Notoginseng,
There is no difference between on wheat and on tobacco, respectively 42.67 and 41.27.
It takes temperature to 5 kinds of vector plant odd-numbered day farrowing measurements bright, in the 16d for starting farrowing, cigarette aphid is on 5 kinds of host plants
Per day farrowing amount have a peak period, wherein chrysanthemum Radix Notoginseng is 3-10d, rape is that 4-13d, wheat are that 3-10d, eggplant are
3-9d, tobacco 4-12d.Cigarette aphid per day farrowing scale in peak period of farrowing on 5 kinds of host plants shows significant difference, chrysanthemum three
Seven be 5.095, and rape is 5.794, and wheat is 5.0131, eggplant is 4.806, tobacco is 4.615.It is comprehensively compared
Per day farrowing amount discovery in the time and farrowing peak period that peak period occurs of farrowing, chrysanthemum Radix Notoginseng all tend to median, oil
Dish is most suitable for cigarette aphid parasitism, and eggplant and tobacco slightly fall behind.
Influence of the 2.3 different host plants to cigarette aphid reproductive speed
Reproduction speed of the 2 cigarette aphid of table on 5 kinds of different host plants
| Different host plants | 1d | 3d | 5d | 7d | 9d | 11d |
| Chrysanthemum Radix Notoginseng | 4 adult aphids | 24 | 77 | 143 | 350 | 500 |
| Rape | 4 adult aphids | 22 | 66 | 220 | 420 | 580 |
| Eggplant | 4 adult aphids | 22 | 59 | 165 | 294 | 385 |
| Wheat | 4 adult aphids | 26 | 65 | 144 | 272 | 432 |
| Tobacco | 4 adult aphids | 35 | 64 | 145 | 370 | 464 |
Note: record data are all cigarette aphid numbers.
From in table 2 it can be seen that reproduction speed significant difference of the cigarette aphid on different host plants.Data after 2 days are shown
Tobacco is more suitable for the farrowing of cigarette aphid initial stage；It is then that chrysanthemum Radix Notoginseng is leading after 4 days, shows that the agreeable to the taste ability of cigarette aphid on it is stronger；6 days and
Data later all show that rape is the host plant most useful for cigarette aphid fast-propagation, and chrysanthemum Radix Notoginseng is compared with tobacco, breeding speed
Faster, eggplant is most slow for degree.
The influence that 2.4 different host plants develop aphidius gifuensis
Fig. 2 is hundred stiff aphid weight statistical charts that aphidius gifuensis is obtained on different host plants.
Note: different English lower cases are marked in figure indicates significant difference (p < 0.05, Deng Kenshi duncan's new multiple range method).
Figure it is seen that the influence significant difference that different host plants generates the development of aphidius gifuensis.On wheat
Stiff aphid it is obviously higher than the stiff aphid weight on other four kinds of host plants, have as many as twice with rape disparity.Followed by its
To the development of aphidius gifuensis, there is also notable differences between his 3 kinds of host plants, and the stiff aphid development mainly on rape is smaller,
Difference is not significant between aphidius gifuensis development on his three kinds of host plants.
2.5 major vital table parameter
Population life table parameters of the 3 cigarette aphid of table on different hosts
| Parameter | Chrysanthemum Radix Notoginseng | Wheat | Rape | Eggplant | Tobacco |
| Net breeding rate R0 | 52.334 | 69.267 | 49.132 | 42.668 | 41.262 |
| Finite rate of increase λ | 1.319 | 1.320 | 1.295 | 1.327 | 1.290 |
| Intrinsic rate of increase rm | 0.277 | 0.278 | 0.280 | 0.283 | 0.255 |
| Average generation cycle T/d | 14.311 | 14.930 | 14.331 | 13.278 | 14.599 |
| Population doubling time t/d | 2.506 | 2.497 | 2.609 | 2.452 | 2.720 |
As shown in Table 3, net breeding rate R0, maximum (69.267), followed by chrysanthemum Radix Notoginseng (52.334) on wheat, on tobacco
Minimum (41.262).Finite rate of increase λ is all larger than 1, shows that the population of cigarette aphid increases in geometric type, is ordered as eggplant by its value size
Sub- > wheat > chrysanthemum Radix Notoginseng > rape > tobacco, wherein chrysanthemum Radix Notoginseng and biennial radish only poor 0.001.Intrinsic rate of increase rm is indicated
Cigarette aphid is consistent with the trend of λ value to the fitness and palatability of host plant, shows under the same terms, cigarette aphid is easier to take
Eggplant, wheat and chrysanthemum Radix Notoginseng are eaten, the tobacco under the experiment condition is likely to be pest-resistant cultivar.Average generation cycle T is then eggplant
Most short (13.278), other 4 kinds of vector plants are all larger than 14d, and the population doubling time is in 2.5d or so.Overall merit, chrysanthemum three
Seven on parameters all in middle position, can be used as the common host plant of substitution and expand numerous cigarette aphid and aphidius gifuensis.
(2) vector plant Cultivating techniques
Pass through and carry out female root propagation, more hibernaculum breeding, cutting propagation effect rating test, it was demonstrated that can be in the way of cuttage
Realize that the scale of vector plant chrysanthemum Radix Notoginseng quickly produces.
Related multiplication technique are as follows:
(1) three-dimensional to expand numerous frame: to make the numerous bee frame of nursery of 160 × 60 × 180cm of shelf angle iron, be divided into inside frame body
Four layers, i.e., respectively away from ground 20,60,100,140cm punishment every.Every layer of separation overhung LED light, meets chrysanthemum Radix Notoginseng, cigarette
Aphid, aphidius gifuensis grow demand.The seedling bed of 150 × 50 × 20cm is made of sheet metal, intermediate every 50cm, which is done, to be separated, i.e., each
Seedling bed is divided into the small nursery section of 3 50 × 50cm.Every numerous bee frame of nursery places 4 seedling beds.
(2) using vermiculite, perlite, turfy soil as matrix, the base of thickness 8-10cm seedling medium: is laid in seedling bed
Matter strikes off, and carries out sterilization with 0.5% liquor potassic permanganate.
(3) cutting and method: taking mature tender stem cuttage from healthy and strong chrysanthemum Radix Notoginseng mother's stem, cutting long 10cm, 3-5 piece leaf,
Pluck radical leaves and insert in seedbed, each seedling bed can 150 plants of chrysanthemum Radix Notoginseng of cuttage, moisturizing of shading.
Cuttage should select the branch of growth 4-6 weeks in winter and early spring room, and summer cuttage should select to grow long 3-5 weeks branch
Item, growth time are more than that the branch of 60d or more can only take the part on its nearly top.The branch lower end thickness of selection should be as much as possible
It is meticulous to take root slow and lack greater than 0.3cm.The spigot of cutting cuts flat with inclined-plane that is neat or being cut into 450 jiaos, cutting with sharp cutter
Length be advisable (bud point, which can be plucked, to be plucked) with 8-10cm, upper end retains the blade of 1/4-1/3, remaining blade all cuts,
Nutritional ingredient in branch can be reduced by doing so consumes in large quantities, is also conducive to the differentiation of calli induction and root.
(4) cutting time: throughout the year can cuttage, but from the viewpoint of production, the time of cuttage can be by current year
December to the March of second year between carry out.The carbendazim that the cutting concentration got ready is 0.5% is first impregnated into 20- before cuttage
30min, taking-up are rinsed well with clear water, the cuttage again after the moisture on its surface dries.First slotting machine is ditched when cuttage, Huo Zheyong
Small wooden pricks cave, and depth is 3-4cm, then cutting is put in, and righting is simultaneously filled out sand and pushed down, and spacing 5-7cm guarantees root growth
There are enough spaces more.Multirow cuttage keeps line-spacing 8-10cm, and every seedling bed can 150 plants of cuttage chrysanthemum Radix Notoginseng.Cuttage finishes water spray,
Make loose matrix natural sediment, is finally covered tightly with transparent plastic foil, moisturizing.
(5) expand numerous management: temperature should keep 20-26 DEG C, and the relative humidity in bed should keep 75% or so, and temperature is excessively high to answer
Pay attention to ventilation, sprays a small amount of water in cutting every 1-2d, the humidity in vitality and bed to keep cutting.Normal item
5-7d initially forms callus under part, and gradually expands, and has small (indefinite) root from the edge of callus using 15d or so
Or central part differentiates and, more slightly the cutting of (0.5cm) can differentiate more than 10 item roots in 2-3d.When the length of root reaches
When 5cm, water in suitable control slotting machine reduces the humidity in bed, prevents the excessive excessive growth of root system, the common light of multi-pass wind is allowed to
Gradually reform of nature environment.
(3) saturation type connects the numerous bee technology of aphid, batch
(1) host selects:
Select host --- the cigarette aphid of aphidius gifuensis advantage parasitism.
(2) conservation maintains: i.e. the foundation and maintenance of conservation population.
Expand the needs of numerous to meet, the conservation population of cigarette aphid and aphidius gifuensis need to be established, the suitable conservation ring of this two population
Border condition are as follows: 25 ± 1 DEG C of day temperature, 20 ± 1 DEG C of nocturnal temperature, relative humidity RH65%-70%, photoperiod L: D=14h:
10h, intensity of illumination 5000Lx.Periodically rejuvenation is carried out from field acquisition original inhabitants population.
(3) it is inoculated with opportunity:
(about 20d growth period) access aphid when the chrysanthemum Radix Notoginseng of cuttage restores to grow 10 blades, monolithic blade face can at this time
Negative worm 400.Unit area larva number is 35/cm2Left and right, is optimal opportunity.
(4) saturation connects aphid:
Every plant chrysanthemum Radix Notoginseng cigarette receiving aphid adult aphid 50 or so, make its oviposition.After 7d, cigarette aphid quantity is reachable on every plant of chrysanthemum Radix Notoginseng
4000 or so, every seedling bed can numerous aphid 600,000, every totally 4 layers of the numerous bee frame of nursery, fertile aphid 2,400,000 or so, realize
Saturation type connects worm.
(5) cigarette aphid, aphidius gifuensis yield:
Greenhouse (200m2) can place and bring up 30, Miao Fan bee frame, aphid 72,000,000 or so can be bred.
Bee 30,000 (sex ration=2: 1, daily 6000, continuous 5d) are put, during which Aphed population can still increase, cigarette aphid cocoon
About 11d completes a generation at 28 DEG C of bee, and per female oviposition 230, high temperature guarantees that property is than being about that (queen bee is more by 1.2:1 under nutritional condition
It is more), hatching rate 90%, aphidius gifuensis can expand numerous 2 generation after 25d, and every plant of chrysanthemum Radix Notoginseng of stiff aphid amount about 2000, every greenhouse can give birth to
Produce stiff aphid 36,000,000 or so (2000 heads/plant × 150 plant/layer × 4 layer/frame × 30 framves).
Note: theoretically after the first generation queen bee amount up to 2,100,000 [=30000 × 2/3 (female ratio) × 200 × 90%
× 0.6 (offspring queen bee ratio)=2,160,000], second generation queen bee amount theoretical value up to 2.33 hundred million [2,160,000 × 200 × 90% ×
0.6], but actually since the death rate, developmental rate, nutrition supply, space limitation etc. influence, current multiplication technique cannot still reach
To the level.
(6) batch expands numerous:
It cultivates seedling respectively according to the above method at interval of 25d, connects worm, connects bee, realize the batch production of anniversary relay,
Meet prevention and control requirement, guarantees product supply.
(7) technical combinations:
By repetition test, summary expands numerous aphidius gifuensis optimal combination by vector plant of chrysanthemum Radix Notoginseng using cottage method
Are as follows:
The numerous bee frame of three-dimensional seedling raising: 4 layered tower stereoscopic frames, soilless substrate.
Cuttage chrysanthemum Radix Notoginseng: the long 10cm of chrysanthemum Radix Notoginseng cutting, depth are 3-4cm, spacing 5-7cm, line-spacing 8-10cm, every nursery
Bed can 150 plants of cuttage chrysanthemum Radix Notoginseng.
Environmental condition: 25 ± 1 DEG C of day temperature, 20 ± 1 DEG C of nocturnal temperature, relative humidity RH65%-70%, photoperiod L:
D=14h: 10h, intensity of illumination 5000Lx.
Expand numerous aphid parasite: 7d+ cigarette receiving aphid parasite 50,000 is cultivated in+50 cigarette aphids of chrysanthemum Radix Notoginseng/strain that growth period is 20d, continuously
Cultivate 25d.
Results of measuring is: in 50d in two stages, first is that the cutting propagation stage of vector plant chrysanthemum Radix Notoginseng, second is that cigarette aphid and
Aphidius gifuensis reproductive stage, the two stages can synchronize progress.In this way, every 25d is in 200m in production season2Greenhouse in, can
Expand numerous aphidius gifuensis 36,000,000.
Above-mentioned technical combinations can greatly save numerous bee cost, can promote large-scale breeding or even the commercialization of aphidius gifuensis
Production.
Experiment 2: expand numerous aphidius gifuensis using chrysanthemum Radix Notoginseng and test
1: determining the test for most preferably connecing aphid opportunity
Test method: each 1 alms bowl immigration of the chrysanthemum Radix Notoginseng in 12d, 16d, 20d, 24d, 28d growth period after cuttage is connect into worm cage respectively
In (30 × 30 × 30cm), and 50 cigarette aphids are accessed into cage, then will connect worm cage and be placed in growth cabinet.It is counted after 3 days
Each processing Aphed population, palatability of the evaluation cigarette aphid to different growing stages chrysanthemum Radix Notoginseng.Totally 5 repetitions.
Test result: cigarette aphid has significant difference to the chrysanthemum Radix Notoginseng hobby property of different growing stages, and all in all, cigarette aphid is more
Feeding 20d growth period blade is liked, quantity reaches 620 or so, is significantly higher than other processing；The chrysanthemum of 16d, 24d, 28d after cuttage
Sanchi leaf on piece is averaged, and to carry aphid amount minimum for aphid number leaf of Radix gynurae segeti piece after 300 or more, cuttage 12, has between each processing significant
Sex differernce.It has been observed that the leaf of Radix gynurae segeti on piece of cuttage 24d, 28d, aphid is concentrated mainly on middle and upper part blade, and radical leaves carry
Aphid amount is seldom.
In view of factors such as toxigenic capacities, in production with the chrysanthemum Radix Notoginseng of cuttage 20d be it is excellent.
Selectivity of the 4 cigarette aphid of table to different growing stages growth period chrysanthemum Radix Notoginseng
| Growth period | Total larva number (head) |
| 12 | 182±46d |
| 16 | 336±81c |
| 20 | 624±75a |
| 24 | 415±68b |
| 28 | 338±14c |
Note: data are mean+SD in table；Different lowercases indicate the significance of difference in 0.05 level
2: judgement most preferably connects the test of Aphed population
Test method: each connecing 1 plant of chrysanthemum Radix Notoginseng that worm cage (30 × 30 × 30cm) is put into after cuttage 20d, if 5 processing,
5 repetitions of every processing.10,30,50,70,90 cigarette aphids are accessed into cage respectively, each processing aphid will be observed after 7d
Aphid amount is storaged in quantity, estimation unit plane.
Test result: with increasing for access aphid, the raised trend of aphid total amount after 7d is generally presented.Comprehensive analysis
It has been shown that, connect aphid amount it is how low when, such as 10,30, and underuse chrysanthemum Radix Notoginseng effective blade and carry aphid space；When 50 cigarettes of access
When aphid adult, total aphid amount, unit plane stowage aphid amount can reach 4000,35/cm2Level, be not only avoided that intraspecific competition, but also
The cigarette aphid of a large amount of health can be obtained；When accessing 70,90 cigarette aphids, since insect density is excessively high, after 7d, Aphed population is not
It is significantly higher than 50.
Therefore, the most suitable quantity of every pot seedling access cigarette aphid adult is 50, and it is about 35/cm that aphid amount, which is storaged, in unit plane2's
Density.
5 cigarette aphid of table is most suitable to connect borer population amount
| Connect aphid amount (head) | Total aphid amount (head) after 7d | Aphid amount (head/cm is storaged in unit plane2) |
| 10 | 1310±60d | 19c |
| 30 | 2680±125c | 24b |
| 50 | 4020±120b | 35a |
| 70 | 4820±215ab | 39a |
| 90 | 5300±260a | 42a |
Note: data are mean+SD in table；Different lowercases indicate that the difference in 0.05 level is aobvious in table
Work property
3: determining the test for most preferably connecing bee quantity
Test method: on the basis of aforementioned 2 test result, each 1 alms bowl immigration of the chrysanthemum Radix Notoginseng of 20d after cuttage is connect into worm cage
In (30 × 30 × 30cm), and 50 cigarette aphids are accessed into cage, total aphid amount about 600 in each cage after 3d.It is connect respectively into 6 cages
Enter 2 (1: 300), 3 (1: 200), 4 (1: 150), 5 (1: 120), 10 (1: 60), 15 (1: 40) heads are healthy and strong and have
The aphidius gifuensis queen bee of oviposition experience takes out aphidius gifuensis afterwards for 24 hours, stiff aphid quantity is counted after 5d.Go out bee postscript to aphidius gifuensis
It records out bee number and produces female number.3 repetitions of every processing.
Test result: it as shown in table 3, connects bee ratio and significantly affects stiff aphid quantity, also there is certain shadow to offspring's female and male ratio
It rings, but offspring's hatching rate is influenced not significant.It is more appropriate in 1:150-200 than controlling that comprehensive analysis connects bee, is suitable for extensive expand
Numerous needs.Inoculation proportion is too low, causes space waste, and efficiency is lower；Bee is connect than excessively high, is unfavorable for stiff aphid and is formed, and offspring is male
Sex ratio increases, and is unfavorable for field control pest.
Table 6 connects influence of the bee quantity to stiff aphid and hatching aphidius gifuensis
| Connect bee amount (head) | Stiff aphid amount (head) after 5d | Offspring's hatching rate (%) | Offspring's sex ration |
| 2 | 310b | 95a | 1.0ab |
| 3 | 425a | 95a | 1.2a |
| 4 | 475a | 95a | 1.1a |
| 5 | 505a | 90a | 0.9b |
| 10 | 495a | 90a | 0.9b |
| 15 | 510a | 90a | 0.8bc |
Note: data are mean+SD in table；Different lowercases indicate that the difference in 0.05 level is aobvious in table
Work property
(4) intensive numerous bee, automatically collecting natural enemy product technology
(1) intensive numerous bee:
Based on the technical combinations that this patent is summarized, numerous bee cost can be significantly saved, the extensive numerous of aphidius gifuensis is promoted
It grows or even merchandized handling.In recent years, expand in the practice of numerous aphidius gifuensis, we arrange production by following expansion numerous periods: i.e. 1-
20 days, expand numerous cuttage chrysanthemum Radix Notoginseng；It the 21-27 days, accesses and expands numerous aphid；28-50d is accessed and is expanded numerous aphidius gifuensis.In life
Season is produced, 25 days are the period, and alternately each step is average in every 200m2Greenhouse in, numerous aphidius gifuensis 36,000,000 can be expanded
Head.
(2) stiff aphid production bee card is collected:
To collect its stiff aphid to numerous aphidius gifuensis is expanded, and make bee card convenient for storage, transport and release.Main process
Are as follows: using stiff aphid powered brush by under the aphidius gifuensis deadlock aphid brush in leaf of Radix gynurae segeti face, smeared with double-sided adhesive at suitable big on bee card
It is small, the stiff aphid of collection is adhered on double-sided adhesive, the bee card of 200 stiff aphids is made.
It is described above simply to illustrate that of the invention, it is understood that the present invention is not limited to the above embodiments, meets
The various variants of inventive concept are within the scope of the present invention.
Claims (15)
1. a kind of method for expanding numerous aphidius gifuensis using cuttage plant three-dimensional, which is characterized in that this method includes with the next stage:
1) host plant chrysanthemum Radix Notoginseng is cultivated；
2) the numerous aphid of aphid is connect；
3) the numerous bee of bee is connect；
4) stiff aphid production bee card is collected；
Described the 1) in the stage, cultivates chrysanthemum Radix Notoginseng using cottage method, indoors throughout the year can cuttage；
Before described 1) stage, the conservation population of cigarette aphid and aphidius gifuensis is established, and periodically from field acquisition original inhabitants population
Carry out rejuvenation；
The conservation environmental condition that the cigarette aphid and aphidius gifuensis population are suitable for are as follows: 25 ± 1 DEG C of day temperature, nocturnal temperature 20 ± 1
DEG C, relative humidity RH65%-70%, photoperiod L: D=14h: 10h, intensity of illumination 5000Lx；
Entering described the after 7d, 3) stage, cigarette receiving aphid parasite continuously cultivate 25d；
Sex ration when putting bee is 2: 1, daily 6000, continuous 5d；
About 11d completes a generation at 28 DEG C of aphidius gifuensis, and high temperature guarantees that female drone property is than being about 1.2:1, hatching rate under nutritional condition
Aphidius gifuensis can expand numerous 2 generation after 90%, 25d.
2. the method as described in claim 1, which is characterized in that the time of the cuttage is by the December of current year to the 3 of second year
It is carried out between month.
3. the method as described in claim 1, which is characterized in that described the 1), 2), 3), 4) in the stage, expanded using solid numerous
Frame is divided into several layers seedling bed, nursery bed depth 20cm, every layer of seedling bed overhung LED light inside frame body.
4. method as claimed in claim 3, which is characterized in that the seedling medium that the seedling bed uses are as follows: with vermiculite, pearl
Rock, turfy soil are matrix, and the matrix of thickness 8-10cm is laid in seedling bed, is struck off, and carry out sterilizing with liquor potassic permanganate and disappear
Poison.
5. method as claimed in claim 4, which is characterized in that the cutting that the chrysanthemum Radix Notoginseng uses are as follows: from healthy and strong chrysanthemum Radix Notoginseng
Mature tender stem cuttage is taken on female stem, the long 3-5 piece leaf of cutting plucks radical leaves and inserts in seedling bed, moisturizing of shading.
6. method as claimed in claim 5, which is characterized in that condition is chosen in the cutting of the chrysanthemum Radix Notoginseng are as follows: winter and early spring
Indoor cuttage selection grows 4-6 week branch, the branch of Summer Indoor cuttage selection growth 3-5 weeks, growth time be more than 60d with
On branch only take the part on its nearly top；
The branch lower end thickness of selection is greater than 0.3cm, and the spigot of cutting cuts flat with inclined-plane that is neat or being cut into 45 degree of angles, cutting with cutter
Length 8-10cm, upper end retains the blade of 1/4-1/3, remaining blade all cuts.
7. method as claimed in claim 5, which is characterized in that before the cuttage first by the cutting concentration got ready be 0.5%
Carbendazim impregnate 20-30min, taking-up rinsed well with clear water, the cuttage again after the moisture on its surface dries；
First nursery bed substrate is ditched when cuttage, or pricks cave with small wooden, depth is 3-4cm, then cutting is put in, helps
It just and fills out sand and pushes down, spacing 5-7cm reserves the space of root growth；
Multirow cuttage keeps line-spacing 8-10cm；
Cuttage finishes water spray, makes loose matrix natural sediment, is finally covered tightly with transparent plastic foil, moisturizing.
8. the method for claim 7, which is characterized in that the numerous condition of the expansion of the cutting are as follows: temperature should keep 20-26
DEG C, the relative humidity in bed should keep 75%, and temperature is excessively high to should be noted that ventilation, a small amount of water is sprayed in cutting every 1-2d, with
Keep the humidity in the vitality and bed of cutting；
When the length for the root that cutting is grown reaches 5cm, water in suitable control seedling bed reduces the humidity in seedling bed,
The excessive excessive growth of root system is prevented, increases ventilation time and light application time, makes cutting gradually reform of nature environment.
9. the method as described in claim 1, which is characterized in that described the 2) in the stage, and the chrysanthemum Radix Notoginseng to cuttage restores to grow
Aphid is accessed when 10 blades or 20d growth period.
10. method as claimed in claim 9, which is characterized in that monolithic blade face can negative worm 400, unit area larva number is
35/cm2。
11. method as claimed in claim 9, which is characterized in that every plant of chrysanthemum Radix Notoginseng cigarette receiving aphid adult aphid 50 makes its oviposition, 7d
Afterwards, cigarette aphid quantity realizes that saturation type connects worm.
12. the method as described in claim 1, which is characterized in that meet bee aphid ratio 1:150-200 when bee.
13. method as claimed in claim 9, which is characterized in that described the 4) in the stage, to expand numerous aphidius gifuensis collect it is stiff
Aphid, and make bee card.
14. method as claimed in claim 13, which is characterized in that utilize stiff aphid powered brush by the aphidius gifuensis in leaf of Radix gynurae segeti face
Under stiff aphid brush, double-sided adhesive is smeared on bee card, the stiff aphid of collection is adhered on double-sided adhesive, the bee card of 200 stiff aphids is made.
15. the method as described in claim 1, which is characterized in that at interval of 25d according to the above method respectively cultivate seedling, connect worm,
Bee is connect, the batch production of anniversary relay is realized, meets prevention and control requirement, guarantee product supply.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510272588.0A CN105165726B (en) | 2015-05-25 | 2015-05-25 | A method of expanding numerous aphidius gifuensis using cuttage plant three-dimensional |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510272588.0A CN105165726B (en) | 2015-05-25 | 2015-05-25 | A method of expanding numerous aphidius gifuensis using cuttage plant three-dimensional |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105165726A CN105165726A (en) | 2015-12-23 |
| CN105165726B true CN105165726B (en) | 2019-05-10 |
Family
ID=54888610
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510272588.0A Active CN105165726B (en) | 2015-05-25 | 2015-05-25 | A method of expanding numerous aphidius gifuensis using cuttage plant three-dimensional |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105165726B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172248B (en) * | 2016-07-21 | 2017-09-29 | 中国热带农业科学院环境与植物保护研究所 | A kind of expanding propagation method of Japan's food a red-spotted lizard aphid chalcid fly |
| CN107926862A (en) * | 2017-11-15 | 2018-04-20 | 宁波市农业科学研究院 | A kind of trichogramma method for releasing with Hosta ventricosa away from flower for carrier and nectar source |
| CN108207822B (en) * | 2018-03-12 | 2020-10-02 | 内蒙古农业大学 | Artificial mass rapid breeding technical method for Aphidius fusceolatus |
| CN109892132A (en) * | 2019-03-18 | 2019-06-18 | 中国农业科学院烟草研究所 | A kind of artificial natural enemy protection island |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168751A (en) * | 2013-04-07 | 2013-06-26 | 中国农业科学院植物保护研究所 | Expanding propagation method for aphidius gifuensis ashmeads |
| CN104585135A (en) * | 2014-12-21 | 2015-05-06 | 辽宁省烟草公司朝阳市公司 | Overwintering breeding method for aphidiusgifuensis in northern area |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664017B (en) * | 2009-09-30 | 2011-09-28 | 云南省烟草农业科学研究院 | Method for propagating sulzer braconid flies |
| CN103168752B (en) * | 2013-04-07 | 2015-04-15 | 中国农业科学院植物保护研究所 | Method for expanding propagation of aphidius gifuensises by using of schizaphis graminums |
-
2015
- 2015-05-25 CN CN201510272588.0A patent/CN105165726B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168751A (en) * | 2013-04-07 | 2013-06-26 | 中国农业科学院植物保护研究所 | Expanding propagation method for aphidius gifuensis ashmeads |
| CN104585135A (en) * | 2014-12-21 | 2015-05-06 | 辽宁省烟草公司朝阳市公司 | Overwintering breeding method for aphidiusgifuensis in northern area |
Non-Patent Citations (1)
| Title |
|---|
| "菊三七扦插繁殖技术和药用价值";孙伟;《中国野生植物资源》;20070630;第26卷(第3期);第68页右栏最后一段-第69页左栏第4段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105165726A (en) | 2015-12-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101390505B (en) | Encarsia Sophia artificial propagation production method | |
| Tsegaye | On indigenous production, genetic diversity and crop ecology of enset (Ensete ventricosum (Welw.) Cheesman) | |
| CN103703974A (en) | Method for cultivating sweet potatoes | |
| CN103155906A (en) | Aphidoletes aphidimyza mass reproduction technology and application technology | |
| CN105165726B (en) | A method of expanding numerous aphidius gifuensis using cuttage plant three-dimensional | |
| CN104585137A (en) | Method for preventing and curing tobacco pests by ladybird breeding via fennel aphids in field | |
| CN106689066A (en) | Method for quickly breeding aphidius gifuensis | |
| CN102524193A (en) | Large-scale raising method of dominant parasitoid wasps of liriomyza sativae | |
| CN103503836A (en) | Plutella xylostella cotesia rubecula artificial propagation production method | |
| CN103392674A (en) | Method for breeding aphidiidae through flue-cured tobacco seedling cultivation greenhouse | |
| Babytskiy et al. | New findings of pest sciarid species (Diptera, Sciaridae) in Ukraine, with the first record of Bradysia difformis | |
| Hutchings et al. | Glechoma hederacea L.(Nepeta glechoma Benth., N. hederacea (L.) Trev.) | |
| CN105210732B (en) | A method of utilizing predation acarid control tobacco-plant pest-insect | |
| CN103766324A (en) | Method for biologically preventing green plant bugs by peristenus spretus in winter jujube orchard | |
| CN103125246B (en) | Root limiting cultivating method of quickly achieving peach filial generation seed selection | |
| Elevitch et al. | Gliricidia sepium (gliricidia) | |
| CN102440182A (en) | Method of seed selection of Lonicera macranthoides Hand.-Mazz. honeysuckle flower variety capable of budding but not flowering | |
| Singh | Principles and techniques of silkworm seed production | |
| CN103168752B (en) | Method for expanding propagation of aphidius gifuensises by using of schizaphis graminums | |
| Ajayi et al. | Conservation status of Telfairia spp. in sub-Saharan Africa. | |
| Hinds et al. | Growth and yield of zucchini squash (Cucurbita pepo L.) as influenced by a sunn hemp living mulch | |
| CN111066732B (en) | Method for breeding orius sauteri | |
| Goel et al. | Oak tasar culture: aboriginal of Himalayas | |
| CN107242203A (en) | A kind of red method of utilization plant waste leaf indoor culture beans | |
| CN113557912A (en) | Method for interplanting epimedium sagittifolia and economic forest trees |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |