CN111066732B - Method for breeding orius sauteri - Google Patents
Method for breeding orius sauteri Download PDFInfo
- Publication number
- CN111066732B CN111066732B CN201911284236.1A CN201911284236A CN111066732B CN 111066732 B CN111066732 B CN 111066732B CN 201911284236 A CN201911284236 A CN 201911284236A CN 111066732 B CN111066732 B CN 111066732B
- Authority
- CN
- China
- Prior art keywords
- breeding
- feeding
- box
- eggs
- orius
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
Abstract
The invention relates to the field of plant protection and insect breeding technology, in particular to a method for breeding orius minutus by using armyworm eggs as feed.
Description
Technical Field
The invention belongs to the technical field of insect science, and particularly relates to a method for breeding orius sauteri.
Background
Orius sauteri (Poppius) belonging to family Hemiptera of family Oridonicaceae, Anthocidae, is an egg of many small arthropods (e.g., thrips, aphids, spider mites, whiteflies) and various lepidoptera insects (e.g., Heliothis gossypii, armyworms, etc.) and a natural enemy of their first-hatched larvae. Has the characteristics of early occurrence, large amount, long time, miscellaneous eating property, wide distribution, strong activity and the like. Therefore, the orius sauteri has extremely high application value as a natural predator insect of omnivory in a farmland ecosystem.
In order to improve the utilization value of the orius sauteri in the aspect of biological control, scholars at home and abroad research the aspects of life regularity, life habits, biological characteristics, growth and development of the orius sauteri, and particularly try to feed the orius sauteri by using plant source feeds such as artificial ooecium rubrum pupae, thrips, pest mites, aphids and the like, plant pollen, tender corns, beer yeast solution and the like in the aspect of the alternative feed of the orius sauteri, but the feeding effect of the orius sauteri is not ideal, and the large-scale feeding of the orius sauteri cannot be realized. In foreign countries, researchers have implemented large-scale breeding of orius parviflorus by using indian corn borer Plodia interpunctella eggs and mediterranean meal borer Ephestia kuehniella eggs as alternative feeds. Due to the propagation conditions and the limitations of host plants, the two lepidopteran insects do not form a breeding scale in China. The application of the orius minutus in preventing and controlling pests in China is relatively late, the technical research foundation in the aspects of feeding and releasing is still very weak, and no effective feed exists at present.
Aiming at various problems existing in the existing artificial feeding of orius sauteri, in order to better develop the orius sauteri and realize the large-scale feeding of the orius sauteri, the inventor provides a method for feeding the orius sauteri by taking armyworm eggs as feed through long-term experiments.
Disclosure of Invention
In order to solve the problems, the invention provides a method for breeding orius sauteri.
The technical scheme adopted by the invention is as follows:
a method for breeding orius sauteri adopts armyworm eggs as feed to breed the orius sauteri, and mainly comprises the following steps:
(1) selection of oviposition substrates: disinfecting fresh and complete kidney beans purchased in a supermarket by using alcohol on the surface, and putting the kidney beans in an insect breeding box for later use after the alcohol is completely volatilized;
(2) screening of orius sauteri species: breeding adult Oridous avidus collected from a cucumber field with myzus persicae in an insect breeding box to establish a stable population, screening 50 pairs of robust adults which are just emerged, enabling the adults to lay eggs after natural mating, and collecting eggs of the Oridous avidus as production insect seeds for later use;
(3) feeding orius miniatus nymphs: the orius minutus nymphs are raised in a bug breeding room with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D as armyworm eggs, the orius minutus nymphs are placed in a bug breeding box, and 5-10 pieces of fan-shaped filter paper are placed in the bug breeding box; each box contains about 500 nymphs, and the nymphs in the insect breeding boxes are consistent in age;
(4) feeding of orius sauteri adults: feeding adults in a phytotron with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D by using armyworm eggs, taking kidney beans as an egg laying substrate, putting adult orius minutus in an insect feeding box, and putting 5-10 pieces of fan-shaped filter paper in the insect feeding box, wherein each box contains about 500 adults;
(5) collecting the orius minutus eggs: and (3) moving out the kidney beans which have spawned every other day, transferring the kidney beans into an insect breeding box, placing the insect breeding box into an insect breeding chamber with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D for hatching, placing 3-5 kidney beans which have spawned in each box, and continuing to propagate.
The insect-culturing box in the steps (3) and (4) is a sealed plastic preservation box with the specification of 32cm multiplied by 22cm multiplied by 10cm, and the middle of the preservation box cover is sealed by a 100-mesh insect-proof net with the thickness of 15 cm multiplied by 10 cm; the filter paper is qualitative filter paper, is cut into strips, and the strips are folded into a fan shape and are placed in the east Asian orius minutus feeding box, so that the movement space is increased.
The armyworm eggs collected in the current day in the step (3) and the step (4) are used for feeding nymphs and adults of orius minutus, wherein egg strips are placed on the nymphs in an amount of 4 armyworm eggs per day, and egg strips are placed on the adults in an amount of 6 armyworm eggs per day.
The culture method of the myxobolus eggs comprises the following steps:
(1) collecting and feeding armyworm larvae: collecting armyworm larvae from corn, and feeding with artificial feed in an armyworm breeding room with temperature of 25 + -1 deg.C, relative humidity of 70 + -5% and photoperiod of 16L: 8D; breeding 1-3 instar larva in plastic basket, transferring into insect cage after 3 instar, and breeding until the larva is mature;
(2) culturing the armyworm pupae: transferring the mature nymphs into a tray paved with fine sandy soil with the thickness of 10cm, digging the nymphs out of the soil after 3 d, putting the nymphs into a pupation box for eclosion, and sealing the pupation box by using gauze for about 100 heads of each box;
(3) feeding adult myxozoa: feeding adult insects with 10% of honey water by volume ratio, feeding adult insects in an insect breeding cage, and replacing the honey water once a day;
(4) collecting the myxoworm eggs: randomly placing spawning ropes in an adult insect cage for raising adults in the spawning period, replacing the spawning ropes and honey water every day, collecting fresh armyworm eggs, and cutting the eggs into egg strips containing 10-20 eggs.
The size of the plastic basket in the step (1) is 20 cmX20 cm in diameter.
The step (3) of feeding with honey water is to drop honey water onto the absorbent cotton, the absorbent cotton is completely soaked, and the absorbent cotton is placed in a culture dish and then put into an adult feeding cage.
The invention has the beneficial effects that: the armyworm eggs utilized in the method can meet the nutritional requirements of the orius minutus in the whole growth period, the problems existing in the feeding of the orius minutus by using the flour mites, the aphids and the like as feeds are solved, the armyworm large-scale feeding technology is mature, the armyworm eggs are easy to obtain and store, the requirements on production environment are low and the like, the basis on the feeds is provided for the large-scale feeding of the orius minutus, and the feeding effect is good.
Detailed Description
A method for breeding orius sauteri adopts armyworm eggs as feed to breed the orius sauteri, and mainly comprises the following steps:
(1) selection of oviposition substrates: disinfecting fresh and complete kidney beans purchased in a supermarket by using alcohol on the surface, and putting the kidney beans in an insect breeding box for later use after the alcohol is completely volatilized;
(2) screening of orius sauteri species: breeding adult Oridous avidus collected from a cucumber field with myzus persicae in an insect breeding box to establish a stable population, screening 50 pairs of robust adults which are just emerged, enabling the adults to lay eggs after natural mating, and collecting eggs of the Oridous avidus as production insect seeds for later use;
(3) feeding orius miniatus nymphs: the orius minutus nymphs are raised in a bug breeding room with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D as armyworm eggs, the orius minutus nymphs are placed in a bug breeding box, and 5-10 pieces of fan-shaped filter paper are placed in the bug breeding box; each box contains about 500 nymphs, and the nymphs in the insect breeding boxes are consistent in age;
(4) feeding of orius sauteri adults: feeding adults in a phytotron with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D by using armyworm eggs, taking kidney beans as an egg laying substrate, putting adult orius minutus in an insect feeding box, and putting 5-10 pieces of fan-shaped filter paper in the insect feeding box, wherein each box contains about 500 adults;
(5) collecting the orius minutus eggs: and (3) moving out the kidney beans which have spawned every other day, transferring the kidney beans into an insect breeding box, placing the insect breeding box into an insect breeding chamber with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D for hatching, placing 3-5 kidney beans which have spawned in each box, and continuing to propagate.
The insect-culturing box in the steps (3) and (4) is a sealed plastic preservation box with the specification of 32cm multiplied by 22cm multiplied by 10cm, and the middle of the preservation box cover is sealed by a 100-mesh insect-proof net with the thickness of 15 cm multiplied by 10 cm; the filter paper is qualitative filter paper, is cut into strips, and the strips are folded into a fan shape and are placed in the east Asian orius minutus feeding box, so that the movement space is increased.
The armyworm eggs collected in the current day in the step (3) and the step (4) are used for feeding nymphs and adults of orius minutus, wherein egg strips are placed on the nymphs in an amount of 4 armyworm eggs per day, and egg strips are placed on the adults in an amount of 6 armyworm eggs per day.
When the armyworm eggs are adopted to feed the orius minutus, the collected armyworm eggs are used for feeding the nymphs and adults of the orius minutus; the design is mainly that the armyworm eggs collected on the same day have the most sufficient nutrients, and can well meet the growth and development nutritional requirements of the orius minutus.
The oviposition matrix used in the step (1) is the whole complete kidney bean, 1-year-old and 2-year-old orius minutus hardly eat, and the kidney bean pods are absorbed by the thorns to supplement water.
The orius sauteri originally adopted in the step (2) is collected from a cucumber field.
The culture method of the myxobolus eggs comprises the following steps:
(1) collecting and feeding armyworm larvae: collecting armyworm larvae from corn, and feeding with artificial feed in an armyworm breeding room with temperature of 25 + -1 deg.C, relative humidity of 70 + -5% and photoperiod of 16L: 8D; breeding 1-3 instar larva in plastic basket, transferring into insect cage after 3 instar, and breeding until the larva is mature;
(2) culturing the armyworm pupae: transferring the mature nymphs into a tray paved with fine sandy soil with the thickness of 10cm, digging the nymphs out of the soil after 3 d, putting the nymphs into a pupation box for eclosion, and sealing the pupation box by using gauze for about 100 heads of each box;
(3) feeding adult myxozoa: feeding adult insects with 10% of honey water by volume ratio, feeding adult insects in an insect breeding cage, and replacing the honey water once a day;
(4) collecting the myxoworm eggs: randomly placing spawning ropes in an adult insect cage for raising adults in the spawning period, replacing the spawning ropes and honey water every day, collecting fresh armyworm eggs, and cutting the eggs into egg strips containing 10-20 eggs.
The size of the plastic basket in the step (1) is 20cm multiplied by 20cm in diameter.
The step (3) of feeding the honey water refers to that the honey water is dripped on the absorbent cotton, the absorbent cotton is completely soaked, and the absorbent cotton is placed in a culture dish and is placed in an adult feeding cage.
The insect breeding cage used in the feeding and collecting of the myxomatosis eggs selects a 100-mesh insect-proof net to be sewn into a gauze with the specification of 60cm multiplied by 60cm, and takes a solid material as a frame.
The invention adopts armyworm eggs as feed to feed orius sauteri, and has the following beneficial effects:
1. the armyworm is used as a feed for a plurality of natural enemy insects, the requirement on the rising environment is low, the production cost is low, the growth and development are not influenced by seasons, the artificial feeding technology is mature, the mass production of armyworm eggs is easy to realize, and the armyworm egg feed can be a feed for a long time for raising orius minutus;
2. the armyworm eggs contain rich nutritional ingredients such as protein, amino acid and fatty acid, can meet the nutritional requirements of the orius minutus in the whole growth period, and realize large-scale breeding of the orius minutus.
In conclusion, the armyworm eggs utilized in the method can meet the nutritional requirements of the orius sauteri in the whole growth period, the problems existing in the feeding of the orius sauteri by utilizing the flour mites, the aphids and the like as the feed are solved, the characteristics that the armyworm large-scale feeding technology is mature, the armyworm eggs are easy to obtain and store, the requirement on the production environment is low and the like are utilized, the basis on the feed is provided for the large-scale feeding of the orius sauteri, and the feeding effect is good.
Example 1
Feeding orius miniatus nymphs with mythic eggs:
the nymph stage of the orius minutus bred by the myxomycete egg is 13.7d, and the survival rate of the orius minutus bred from the low age to the old age is as follows: nymphs at 1 st (94%), nymphs at 2 st (97%), nymphs at 3 st (96%), nymphs at 4 st (98%), nymphs at 5 th (99%).
Example 2
Feeding the adult orius sauteri with myxoid eggs:
the average life of the adult Ornitus parvifolius bred by the myxozoon ovum is 17.1d, and the average egg laying amount per female is 72.5.
Claims (5)
1. A method for breeding orius sauteri is characterized by comprising the following steps: the method for feeding orius sauteri by using the myxoworm eggs as the feed mainly comprises the following steps:
(1) selection of oviposition substrates: disinfecting fresh and complete kidney beans purchased in a supermarket by using alcohol on the surface, and putting the kidney beans in an insect breeding box for later use after the alcohol is completely volatilized;
(2) screening of orius sauteri species: breeding adult Oridous avidus collected from a cucumber field with myzus persicae in an insect breeding box to establish a stable population, screening 50 pairs of robust adults which are just emerged, enabling the adults to lay eggs after natural mating, and collecting eggs of the Oridous avidus as production insect seeds for later use;
(3) feeding orius miniatus nymphs: the orius minutus nymphs are raised in a bug breeding room with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D as armyworm eggs, the orius minutus nymphs are placed in a bug breeding box, and 5-10 pieces of fan-shaped filter paper are placed in the bug breeding box; each box contains about 500 nymphs, and the nymphs in the insect breeding boxes are consistent in age;
(4) feeding of orius sauteri adults: feeding adults in a phytotron with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D by using armyworm eggs, taking kidney beans as an egg laying substrate, putting adult orius minutus in an insect feeding box, and putting 5-10 pieces of fan-shaped filter paper in the insect feeding box, wherein each box contains about 500 adults;
(5) collecting the orius minutus eggs: moving out the kidney beans which have spawned every other day, transferring the kidney beans into an insect breeding box, putting the insect breeding box into an insect breeding chamber with the temperature of 25 +/-1 ℃, the relative humidity of 70 +/-5% and the photoperiod of 16L:8D for hatching, putting 3-5 kidney beans which have spawned in each box, and continuing to propagate;
the insect-culturing box in the steps (3) and (4) is a sealed plastic preservation box with the specification of 32cm multiplied by 22cm multiplied by 10cm, and the middle of the preservation box cover is sealed by a 100-mesh insect-proof net with the thickness of 15 cm multiplied by 10 cm; the filter paper is qualitative filter paper, is cut into strips, and the strips are folded into a fan shape and are placed in the east Asian orius minutus feeding box, so that the movement space is increased.
2. The method for breeding orius sauteri according to claim 1, wherein the method comprises the following steps: the armyworm eggs collected in the current day in the step (3) and the step (4) are used for feeding nymphs and adults of orius minutus, wherein egg strips are placed on the nymphs in an amount of 4 armyworm eggs per day, and egg strips are placed on the adults in an amount of 6 armyworm eggs per day.
3. The method for breeding orius sauteri according to claim 1, wherein the method comprises the following steps: the culture method of the myxobolus eggs comprises the following steps:
(1) collecting and feeding armyworm larvae: collecting armyworm larvae from corn, and feeding with artificial feed in an armyworm breeding room with temperature of 25 + -1 deg.C, relative humidity of 70 + -5% and photoperiod of 16L: 8D; breeding 1-3 instar larva in plastic basket, transferring into insect cage after 3 instar, and breeding until the larva is mature;
(2) culturing the armyworm pupae: transferring the mature nymphs into a tray paved with fine sandy soil with the thickness of 10cm, digging the nymphs out of the soil after 3 d, putting the nymphs into a pupation box for eclosion, and sealing the pupation box by using gauze for about 100 heads of each box;
(3) feeding adult myxozoa: feeding adult insects with 10% of honey water by volume ratio, feeding adult insects in an insect breeding cage, and replacing the honey water once a day;
(4) collecting the myxoworm eggs: randomly placing spawning ropes in an adult insect cage for raising adults in the spawning period, replacing the spawning ropes and honey water every day, collecting fresh armyworm eggs, and cutting the eggs into egg strips containing 10-20 eggs.
4. The method for breeding orius sauteri according to claim 3, wherein the method comprises the following steps: the size of the plastic basket in the step (1) is 20 cmX20 cm in diameter.
5. The method for breeding orius sauteri according to claim 3, wherein the method comprises the following steps: the step (3) of feeding with honey water is to drop honey water onto the absorbent cotton, the absorbent cotton is completely soaked, and the absorbent cotton is placed in a culture dish and then put into an adult feeding cage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911284236.1A CN111066732B (en) | 2019-12-13 | 2019-12-13 | Method for breeding orius sauteri |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911284236.1A CN111066732B (en) | 2019-12-13 | 2019-12-13 | Method for breeding orius sauteri |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111066732A CN111066732A (en) | 2020-04-28 |
| CN111066732B true CN111066732B (en) | 2021-12-28 |
Family
ID=70314508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911284236.1A Active CN111066732B (en) | 2019-12-13 | 2019-12-13 | Method for breeding orius sauteri |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111066732B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112997972B (en) * | 2021-03-30 | 2022-09-23 | 中国烟草总公司河南省公司 | Ornitus avidus egg preservation method suitable for large-scale breeding |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5799607A (en) * | 1996-08-06 | 1998-09-01 | The United States Of America As Represented By The Secretary Of Agriculture | Culture medium for parasitic and predaceous insects |
| CN1273027A (en) * | 2000-06-15 | 2000-11-15 | 河北省农林科学院植物保护研究所 | Artificial reproduction method for Microplitis mediator (Haliday) |
| CN105075992A (en) * | 2015-07-07 | 2015-11-25 | 华中农业大学 | Method for raising natural enemy insect southern minute pirate bug indoor in great deal and raising box |
| CN106689063A (en) * | 2016-12-07 | 2017-05-24 | 中国科学院动物研究所 | Artificial breeding production method of trichogramma japonicum |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11253069A (en) * | 1998-03-13 | 1999-09-21 | Sumitomo Chem Co Ltd | Amblyseius womersleyi schicha free from short-day reproductive diapause, its production and vermin controlling method using the insect |
| CN104938429B (en) * | 2015-06-30 | 2017-04-05 | 山东省农业科学院植物保护研究所 | A kind of method for raising Orius |
| CN107279475A (en) * | 2017-08-10 | 2017-10-24 | 中国农业科学院植物保护研究所 | A kind of method using the prey artificial feeding predatism stinkbug kept in cold storage |
| CN107581160A (en) * | 2017-09-30 | 2018-01-16 | 贵州省烟草公司遵义市公司绥阳县分公司 | A kind of artificial scale breeding method of mythimna separata |
-
2019
- 2019-12-13 CN CN201911284236.1A patent/CN111066732B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5799607A (en) * | 1996-08-06 | 1998-09-01 | The United States Of America As Represented By The Secretary Of Agriculture | Culture medium for parasitic and predaceous insects |
| CN1273027A (en) * | 2000-06-15 | 2000-11-15 | 河北省农林科学院植物保护研究所 | Artificial reproduction method for Microplitis mediator (Haliday) |
| CN105075992A (en) * | 2015-07-07 | 2015-11-25 | 华中农业大学 | Method for raising natural enemy insect southern minute pirate bug indoor in great deal and raising box |
| CN106689063A (en) * | 2016-12-07 | 2017-05-24 | 中国科学院动物研究所 | Artificial breeding production method of trichogramma japonicum |
Non-Patent Citations (3)
| Title |
|---|
| 东亚小花蝽对草地贪夜蛾幼虫的捕食能力评价;代晓彦等;《中国生物防治学报》;20191031;第35卷(第5期);第704-708页 * |
| 小花蝽的生物学特性与防治棉红蜘蛛的初步试验;邱益三等;《昆虫天敌》;19800731(第2期);第40-47页 * |
| 烟草病虫害绿色防控技术研究进展;邱睿等;《河南农业科学》;20161130;第45卷(第11期);第8-13页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111066732A (en) | 2020-04-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101796940A (en) | Super flour weevil culture technology | |
| CN103168751A (en) | Expanding propagation method for aphidius gifuensis ashmeads | |
| CN102870742A (en) | Large-scale artificial feeding method for eretmocerus hayati | |
| CN110679553B (en) | Large-scale propagation method for Bactrocera cucurbitae larva parasitic wasp Choisy | |
| CN109769755A (en) | A kind of simple and fast propagation method of spotted wing drosophila pupa parasitic wasp hair angle clavicorn serphid | |
| CN110367205A (en) | A method of beautiful Chrysopa is raised using pea tail reparing aphid | |
| CN106879557A (en) | A kind of method for raising black striped plant bug | |
| CN110447604B (en) | Method for improving feeding effect of arma chinensis by adding alternative host plant | |
| Zheng et al. | Development and reproduction of the hoverfly Eupeodes corollae (Diptera: Syrphidae) | |
| CN105165726B (en) | A method of expanding numerous aphidius gifuensis using cuttage plant three-dimensional | |
| CN108013209B (en) | Artificial fermented feed for feeding larvae of Tirathaba rufivena | |
| CN111066732B (en) | Method for breeding orius sauteri | |
| CN111802332B (en) | Method for feeding aphid-eating gall midge by using mixed population of various aphids on barley | |
| CN111543396B (en) | Indoor large-scale breeding method for small flies and pupa terracotta warriors | |
| CN106900657A (en) | A kind of method that use live body host plant expands numerous black striped plant bug | |
| CN107182943A (en) | A kind of method of indoor mass rearing Bradysia fungus gnat | |
| CN107242203A (en) | A kind of red method of utilization plant waste leaf indoor culture beans | |
| CN112471082A (en) | Broad-band optimized aphid fly breeding method | |
| CN107581163B (en) | Method for feeding cryptopterus gracilis by using tetranychus cinnabarinus | |
| CN111034684A (en) | Semitrella semipenguii seed conservation and rejuvenation breeding method | |
| CN106472431B (en) | A kind of collection method of Serangium japonicum pupa and the artificial large-scale breeding method of Serangium japonicum | |
| CN106212397B (en) | A kind of artificial fecundation method of aphidius gifuensis | |
| CN116982681B (en) | Spodoptera frugiperda artificial feed and preparation method thereof | |
| CN114176048B (en) | Method for breeding black oophagous trichogrammae of tung tree looper | |
| CN102144618B (en) | Technology for artificially feeding euploea core on scale alternately in two hosts |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |