CN110367205A - A method of beautiful Chrysopa is raised using pea tail reparing aphid - Google Patents
A method of beautiful Chrysopa is raised using pea tail reparing aphid Download PDFInfo
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- CN110367205A CN110367205A CN201910652561.2A CN201910652561A CN110367205A CN 110367205 A CN110367205 A CN 110367205A CN 201910652561 A CN201910652561 A CN 201910652561A CN 110367205 A CN110367205 A CN 110367205A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- Environmental Sciences (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The present invention relates to a kind of method for raising beautiful Chrysopa using pea tail reparing aphid, this method raises beautiful Chrysopa by prey of pea tail reparing aphid；The following steps are included: beautiful Chrysopa ovum collecting and hatching, beautiful Chrysopa larva group breeding, mature larva concentration pupate to form beautiful Chrysopa cocoon, beautiful Chrysopa adult eclosion and raising.The present invention realizes high-quality and efficient, easy economic, the standardization large-scale production throughout the year of aphid and Chrysopa, it can be used for preventing and treating a variety of aphids, aleyrodid, coccid, harmful mite and the agriculture and forestry injurious insects such as Lepidoptera ovum and low instar larvae, meet the biological controls demand such as facilities vegetable, crop field, orchard and flowers.
Description
Technical field
The invention belongs to the technical field of biological control of agricultural pests more particularly to a kind of utilization pea tail reparing aphid raising are beautiful
The method of Chrysopa.
Background technique
Aphid is the Severe pests in the production of world's agriculture and forestry, blazons monoid for the world, widely distributed in China, wherein with
The distribution of the provinces and cities such as Liaoning, Hebei, Gansu, Xinjiang, Beijing, Yunnan and Taiwan is more intensive.Host range is wide for aphid, number occurs
Amount is big, reproduction speed is fast, generation overlap is serious, can endanger plant by modes such as feeding, honeydew pollution, transmitted virus, make
, wilting slow at plant strain growth, and induce a variety of virosis and black points etc., plant organ's damage and nutrition loss are caused,
It can lead to plant death when serious, and cause the generation and prevalence of plant disease, make agricultural production by great economic loss.
For a long time, the prevention and treatment of aphid relies primarily on absorbability chemical insecticide, but the long-term excessive use of pesticide, leads to the anti-medicine of aphid
Property enhancing, Pesticide use amount persistently increases, and also produces numerous food product safety problem.Therefore, the green bio for carrying out aphid is anti-
Control, especially the ecological agriculture, organic agriculture, nuisanceless green agricultural product production in, pass through natural enemy insect etc. control aphid danger
Evil is not only advantageous to reduce the usage amount of chemical pesticide, protects environment, maintains the ecological balance and protection bio-diversity, and
To agricultural sustainable development is promoted, economic benefit and the ecological benefits for improving agricultural production are significant.
There are many natural enemy insect type of aphid, including Chrysopa, ladybug, wasp fly, aphid parasite etc..Wherein, Chrysopa is prevention and treatment
The important predator of one kind of aphid, widely distributed, many kinds of, larva and most of adult can prey on aphid, and
Predatory number is big, the predation period is long, reproductive capacity is high, has to a variety of aphids such as black peach aphid, soybean aphid, acyrthosiphum pisim, wheat aphid, radish aphid aobvious
Write control effect, in addition, Chrysopa can also prevention and control crop field, orchard, gardening and the indoor aleyrodid of temperature, thrips, tetranychid, coccid and
Lepidoptera pest etc. plays irreplaceable important function in agriculture and forestry production.
Beautiful Chrysopa Chrysopa formosa is a kind of excellent predatory natural enemy insect, because its recipe is wide, heavy, production
Ovum amount is high, the service life is long, is easy for workers to the advantages that breeding, becomes one of very high type of utility value in chrysopidae.The Chrysopa master
It is distributed in Europe and Asia, it is widely distributed in China, belong to local sociales.The larva and adult of beautiful Chrysopa can catch
Food can control pea tail reparing aphid, black peach aphid, soybean aphid, Cereal aphid etc. and aleyrodid, coccid, thrips, mite class, Lepidoptera
The pests such as ovum and low instar larvae are caused harm, the significant effect in the prevention and treatment of the pests such as field crop, fruits and vegetables, flowers.In view of beautiful Chrysopa
Excellent biological and ecological methods to prevent plant disease, pests, and erosion ability, research and develop the efficient multiplication technique of standardization of the Chrysopa, Chrysopa product be mass produced, to the agriculture of prevention and control China
Various pests in forestry are of great significance.But it is reported both at home and abroad about the research of Chrysopa large-scale breeding technology at present
Seldom, each sport technique segment for being related to Chrysopa production such as substitutes prey screening, rearing conditions and method and still needs to Improvement, because
This, it is the problem of producing upper urgent need to resolve that how save the cost, which largely expands numerous Chrysopa,.
Summary of the invention
In view of the problems of prior art, the present invention provides a kind of side that beautiful Chrysopa is raised using pea tail reparing aphid
Method, by optimization aphid, Chrysopa breeding apparatus, condition and method etc., the standardization anniversary for realizing aphid and Chrysopa is largely given birth to
It produces, meets Field information demand.
The technical scheme to solve the above technical problems is that
The present invention provides a kind of method for raising beautiful Chrysopa using pea tail reparing aphid, and this method is using pea tail reparing aphid as prey
Raise beautiful Chrysopa；Beautiful Chrysopa is divided into beautiful Chrysopa ovum, beautiful Chrysopa larva, prepupa, beautiful Chrysopa cocoon in beautiful Chrysopa cocoon according to growth phase
Interior pupa, beautiful Chrysopa adult；Beautiful Chrysopa method for breeding is the following steps are included: beautiful Chrysopa ovum collecting and hatching, beautiful Chrysopa larva group
Raising, mature larva concentration pupate to form beautiful Chrysopa cocoon (successively development forms prepupa and pupa in cocoon), beautiful Chrysopa adult eclosion
And raising.
The beneficial effects of the present invention are: the present invention, which uses pea tail reparing aphid to raise beautiful Chrysopa for prey, has expansion numerous period
Short, production is not subject to seasonal restrictions, and space utilization rate is high, and yield is big, the easy quick, standard criterion of operating process, low in cost etc.
Advantage.To realize the high-quality and efficient long-term production of beautiful Chrysopa, commercially producing and being widely applied for beautiful Chrysopa is promoted.It is excellent
Selection of land, under the conditions of 26 ± 1 DEG C, 16L:8D (daylight 16 hours, night is 8 hours dark), RH70%, with pea tail reparing aphid
Beautiful Chrysopa, which is raised, for prey is conducive to the production of beautiful Chrysopa using the rearing conditions.
Further, beautiful Chrysopa ovum collecting and the method for hatching the following steps are included: 26 ± 1 DEG C, under the conditions of 16L:8D, will be at
Worm pea tail reparing aphid even generation raising, establishes and stablizes population；From new emerging adult lays eggs day, daily timed collection is newly laid eggs,
Ovum grain is placed in bottom to be covered in the box body of raising box of folded filter paper item, according to every 6000cm3Raising box body accumulate and set 80-
The ratio of 100 ovum places beautiful Chrysopa ovum, and the box cover for raising box is equipped with through-hole and is covered with nylon gauze, and nylon gauze has saturating
Stomata (structure can be as shown in Figure 1)；Hatching culture later, condition are 26 ± 1 DEG C, 16L:8D, RH70 ± 5%；It is 4-5 days general
Chrysopa ovum is largely hatched as 1 instar larvae, from hatching day to 1 instar larvae, the daily enough aphids of timing feeding, averagely every larva afterwards
30-80 aphids of feeding daily.
Beneficial effect using the above scheme is: the setting of folded filter paper item can prevent Chrysopa larva from killing each other.It is logical
Air circulation can be guaranteed by crossing air hole and nylon gauze covering, be conducive to beautiful Chrysopa ovum raising.Rigid larvae appetite is small,
Feeding suitable aphid can satisfy growth and development needs.
Further, the method for beautiful Chrysopa larva group breeding is the following steps are included: the daily every average feeding of 2 instar larvaes
100-150 aphids, 3 instar larvaes daily every averagely feeds 120-200 aphids；And guarantee feeding environment cleaning.
Beneficial effect using the above scheme is: with the growth and development of 1 instar larvae, appetite is gradually increased, and is needed before
The feeding situation of larva on the one increases aphid scale of feeding.2-3 instar larvae appetite explodes, after the enough band aphid semen viciae fabae seedlings of daily iron supplement,
The residues such as the residual branch of plant, the aphid corpse in raising box need to be cleared up in time, are guaranteed the cleaning of feeding environment, are prevented humidity in box
Excessively high generation mould.Under above-mentioned rearing conditions, be conducive to larva growth, being developed to 3 age mature larvas from 1 instar larvae only needs 8-10
It.
Further, mature larva, which is concentrated, pupates to form the method for beautiful Chrysopa cocoon the following steps are included: guaranteeing nutrition, to 3 ages
Larva first passes through the prepupa stage in cocoon and is developed to pupa again, judges that the standard pupated is when the visible black husking in cocoon end
When, prepupa develops pupa, which about needs 5-8 days or so；After mature larva starts to cocoon, collect day by day newly formed it is beautiful
Chrysopa cocoon is relayed in new raising box, and is marked and collected date and quantity, for product packaging or expands numerous use.
Further, beautiful Chrysopa adult eclosion and the method for raising the following steps are included: 26 ± 1 DEG C, under the conditions of 16L:8D, will
The beautiful Chrysopa cocoon newly collected, which is placed in dependent insect cage, carries out concentration emergence, according to every 216000cm3The dependent insect cage of volume places 400-
The ratio of 500 cocoons places beautiful Chrysopa cocoon；Beautiful Chrysopa starts to turn into adult after pupating for 8-10 days or so, and new emerging adult is through filling
After dividing feeding, need can lay eggs within 4-6 days；Basin band aphid semen viciae fabae seedling is placed in rearging cage for Adult worms producting eggs, institute's outdoor rearing of tussah silkworm bean seedlings are 10-
12 ages in days carry aphid 50-80 head with every young plant, transfer them in new rearging cage after collecting enough beautiful Chrysopa ovum to egg hatching
Or production ovum card is collected for ovum grain, while replacing new band aphid semen viciae fabae seedling for Adult worms producting eggs.
Beneficial effect using the above scheme is: adult has phototaxis, and general happiness is at the top of dependent insect cage at close to sources
Aggregation.Basin band aphid semen viciae fabae seedling is placed in rearging cage for Adult worms producting eggs, institute's outdoor rearing of tussah silkworm bean seedlings are 10-12 age in days, be conducive to Chrysopa at
Worm inhabites, supplements the nutrients and lays eggs；It carries aphid amount is advisable for aphid 50-80 with every young plant load, and Chrysopa does not like when load aphid amount is excessive
It lays eggs thereon.
A kind of method for breeding of aphid, comprising the following steps: (1) semen viciae fabae is selected to plant as the host for expanding numerous pea tail reparing aphid
Object carries out nursery；(2) aphid is inoculated with and expands numerous.
The beneficial effects of the present invention are: realizing high-quality and efficient pea tail reparing aphid, easy economy, standard by the above method
Change large-scale production throughout the year.Semen viciae fabae is selected to be conducive to the production of raising pea tail reparing aphid as the host plant for expanding numerous pea tail reparing aphid
Amount.
Based on the above technical solution, the present invention can also be improved as follows.
Further, in step (1), selecting kind is the semen viciae fabae of natural king as host plant, the method for nursery include with
Lower step: it 25 ± 1 DEG C, under the conditions of photoperiod 16L:8D, the vermiculite of sterilization treatment is put into seedling-growing container, screens big full
Broad Bean Seeds are uniformly sprinkling upon on vermiculite, according to every 600cm2The ratio nursery of 200-220 semen viciae fabaes is sowed, and on Broad Bean Seeds
Evenly laid out one layer of vermiculite (there just be not Broad Bean Seeds to be advisable with vermiculite), there just was not Broad Bean Seeds to be advisable with vermiculite；Watering is to protect
Holding vermiculite surface layer and moistening is advisable, and cultivates.
Beneficial effect using the above scheme is: inventor selects kind to be used as the semen viciae fabae of natural king and posts by screening
Main plant, the Broad Bean Seeds of the kind have many advantages, such as that full, germination percentage is high, emergence is neat, aphid is fond of, it is high to carry aphid amount, are
The good host plant for expanding numerous pea tail reparing aphid, advantageously ensures that the yield of pea tail reparing aphid.In step (1), suitable temperature
And illumination condition is conducive to Broad Bean Seeds germination fastly, sprout Qi Erzhuan, and seedling branch is early, fast growing is vigorous.Suitably broadcast
Kind of grain number is conducive to balanced nutritious, guarantees the nutrient supply of every seed, keeps the development of semen viciae fabae seedling strong, increase branch amount and blade
Number.
Further, step (2) aphid is inoculated with and expands numerous the following steps are included: in 25 ± 1 DEG C, photoperiod 16L:8D condition
Under, it carries out connecing aphid for the first time when semen viciae fabae seedling plant height reaches 7-9cm after semen viciae fabae after planting 7-8 days, connecing aphid amount is every young plant 4-6 aphids
Worm；If aphid amount is on the low side, may be selected to be connect aphid for the second time within 2-3 days after connecing aphid for the first time, connecing aphid amount is every young plant 10-15 head
Aphid；15-18 days, aphid was covered with semen viciae fabae seedling stalk and leaf front and back sides, at this time the load aphid amount about 300-500 of every plant of semen viciae fabae seedling
Head, about 60000-70000 aphid of each seedling-growing container, during which, watering.
Beneficial effect using the above scheme is: in step (2), by connecing aphid for the first time, generating semen viciae fabae seedling to aphid
The Resistant reaction of worm.Aphid is connect for the second time, it is therefore an objective to be increased aphid inoculation radix, be promoted the rapid, high volume of aphid to expand numerous.Selection
Aphid opportunity suitably is connect, the growth and development for being conducive to balance semen viciae fabae seedling and aphid will lead to semen viciae fabae seedling if connecing aphid too early
Slow growth is even wilted death, aphid due to subalimentation can not mass propagation, if connecing aphid too late will lead to aphid not
The problems such as it is poor to like feeding aging semen viciae fabae seedling, escape, colonization, population can not be established.Selection suitably connects aphid amount and is conducive to
The nutrition supply for guaranteeing semen viciae fabae seedling maintains single plant load aphid amount, aphid Individual Size in OK range, the numerous efficiency of raising expansion,
Save production cost.If connecing aphid amount excessively and will lead to semen viciae fabae seedling single plant carries that aphid amount is excessively high, and density is excessive, aphid individual becomes
Small, alatae quantity increases, the problems such as fleeing of migrating；If it is low to connect the very few aphis population radix that will lead to of aphid amount, semen viciae fabae seedling
Grow it is too fast, produce aphid total amount it is few, aphid expand numerous low efficiency the problems such as.During the growth and development of semen viciae fabae seedling and connecing aphid, according to interior
The wet degree of vermiculite in temperature, humidity condition and seedling-growing container is watered to semen viciae fabae seedling in time, in case water shortage influences semen viciae fabae Miao Sheng
Long and aphid is expanded numerous.
It further, further include that step (3) aphid batch expands numerous step, operating method are as follows: every 3-5 days are a batch, weight
Multiple above-mentioned nursery connects aphid, the numerous operation of aphid expansion.
Beneficial effect using the above scheme is: the setting of step (3) may be implemented continuity batch expand it is numerous, for expand it is numerous
Natural enemy insect provides sufficient stable aphid supply amount.
The present invention provides a kind of for raising the device of beautiful Chrysopa, including box body and box cover；Box body top opening, in box body
Portion is provided with folded filter paper item；Box cover covers at the top of box body, and box cover is provided with through-hole, and yarn is covered at lead to the hole site
Net, the gauze have air hole.
Beneficial effect using the above scheme is: folded filter paper item is conducive to Chrysopa larva and inhabites, and prevents larva certainly mutually residual
It kills, cocooing to pupate for larva provides place, and the gauze with air hole is conducive to ventilation, prevents humidity mistake in raising box
It is big to generate mould, influence Chrysopa growth and development.
The present invention also provides a kind of beautiful Chrysopa packing method, beautiful Chrysopa is divided into beautiful Chrysopa ovum, beautiful Chrysopa according to growth phase
Prepupa in larva, beautiful Chrysopa cocoon, pupa, beautiful Chrysopa adult in beautiful Chrysopa cocoon；Manner of packing is selected according to the growth phase of beautiful Chrysopa；
Beautiful Chrysopa ovum is packed using three-dimensional paper cassette, and beautiful Chrysopa larva uses bottle packaging, and beautiful Chrysopa cocoon (such as: it is pre- in beautiful Chrysopa cocoon
Pupa in pupa, beautiful Chrysopa cocoon) using three-dimensional paper card or bottle packaging, beautiful Chrysopa adult is using box-packaged.
Beneficial effect using the above scheme is: suitable manner of packing is selected according to the different growth phase of beautiful Chrysopa,
Be conducive to the survival and breeding of beautiful Chrysopa, guarantee product quality, is produced into convenient for product storage, transport and release application, reduction
This.
Further, beautiful Chrysopa ovum is using the method for three-dimensional paper cassette packaging the following steps are included: by beautiful Chrysopa ovum from egg pedicle
On cut, collect, beautiful Chrysopa ovum grain is gently swept to be pasted with double faced adhesive tape can doubling be fitted into the three-dimensional paper card of ridge
Two sides, according to every 12cm2The ratio that double-sided adhesive places ovum grain number 800-1000 is packed, and each solid paper card ovum grain is total
Number is 1600-2000.
Beneficial effect using the above scheme is: is conducive to save packaging space using the method that three-dimensional paper cassette is packed,
It is easy to transport and hang release, protection ovum grain is injury-free, guarantees ovum survival and development.The ovum grain number of ovum card is 1600-2000
Grain is conducive to make full use of space, reduces packing cost, if ovum grain number is excessively easy to cause crowded, influences hatching rate and deposits
Problem living, if ovum grain number is very few to be easy to cause waste packaging space problem.
When packaging, every 12cm2Ovum card places ovum grain number 800-1000, and exploration discovery in research is compared using the ratio
Properly, if ovum grain number is less than 800, ovum card area is not fully utilized, and causes space waste, and every ovum card is theoretically maximum
Capacity is 1200 ovum, but actual verification is found, because ovum grain size has differences, 12cm2When ovum card accommodates 1000 ovum
Space is made full use of, and can guarantee that every ovum stabilization is pasted on ovum card.
Further, beautiful Chrysopa larva uses bottle packaging method the following steps are included: selection development age consistent beautiful grass
Sandfly larva is packed, and beautiful Chrysopa larva is placed in the transparent plastic bottle filled with buckwheat shell or rice shell, is set among bottle cap
There is air hole and covered with nylon grenadine, 1 instar larvae presses every 400cm3Bottle body accumulate set the ratio of 1 instar larvae of 400-500 head into
Row packaging, 2 instar larvaes press every 400cm3Bottle body, which accumulates, to be set the ratio of 2 instar larvae of 200-300 head and is packed, and 3 instar larvaes are by every
400cm3Bottle body, which accumulates, to be set the ratio of 3 instar larvae of 100-200 head and is packed.
Beneficial effect using the above scheme is: it is convenient using bottle packaging method simple economy, be conducive to larva storage,
Transport and field adaptability protect larva from damage.It is equipped with air hole among bottle cap and is covered with nylon grenadine and is conducive to ventilation
Ventilation guarantees the breathing of Chrysopa larva.Suitable packaging larva number is conducive to make full use of space, prevents larva from killing each other.
When beautiful Chrysopa was in for 1 instar larvae phase, individual it is smaller, packed bulk density less than 500, survival rate 90% with
On, but when packed bulk density is 600, autotomy rate increases, and survival rate is decreased obviously.But when packed bulk density is 300, Packaging Bottle
Space is underutilized, so the convenient packed bulk density of 1 instar larvae is every bottle of 400-500 head.When beautiful Chrysopa was in for 2 ages
When larval phase, increase with packed bulk density, the decline of larva average viability, when density is increased to 400 every bottle, survival rate only has
79.08%.When beautiful Chrysopa was in for 3 instar larvae phase, individual is larger, and autotomy rate increases, when packed bulk density 100,200,300
Survival rate is respectively 94%, 92% and 82.78%.
Inventor has carried out a large amount of screening experiment, has filtered out convenient packed bulk density of each age, i.e., by every
400cm3Bottle body product can place 1 instar larvae 400-500 head, 2 instar larvae 200-300 heads, and 3 instar larvaes 100-200 are packed.
Packaged beautiful Chrysopa larva can transport directly to field adaptability application.
Further, beautiful Chrysopa cocoon is using the method for three-dimensional paper card packaging the following steps are included: beautiful Chrysopa cocoon is collected, by beautiful grass
Sandfly cocoon is gently swept on the three-dimensional paper card two sides for being pasted with double-sided adhesive, and cocoon card is fabricated to, by every 12cm2Cocoon card places 50-70 cocoons
Ratio packed；Beautiful Chrysopa cocoon is using the method for bottle packaging the following steps are included: being placed in beautiful Chrysopa cocoon filled with buckwheat
In the transparent plastic bottle of wheat or rice shell, by every 400cm3Bottle body, which accumulates, to be set the ratios of 500-1000 cocoons and is packed.
Beneficial effect using the above scheme is: it is convenient using above-mentioned manner of packing simple economy, be conducive to save the cost,
Convenient for product storage, transport and release application, the suitable cocoon amount that fills is conducive to make full use of space, guarantees the quality of cocoon, prevents
Transition is crowded to cause cocoon to damage.
Beautiful Chrysopa cocoon averagely long 4.3mm, wide 3.5mm, according to cocoon size and cocoon card area, theoretically every 12cm2Cocoon card can
Accommodate 60-90 cocoons.During virtual package, cocoon is pasted on paper card at random, quantity causes packaging space when being less than 50
When wasting, and packing cocoon number higher than 70, the normal emergence of pupa is influenced, adult eclosion rate average out to 85.7% compares illumination and shows and drops
It is low.Therefore, every 12cm2Cocoon card place 50-70 cocoons it is convenient, manufactured cocoon card be uniformly placed into packing box (long 20cm, it is wide
10cm, high 8cm) in, every box places 40 cocoon cards.Or cocoon is placed in filled with buckwheat or rice by larva bottle packaging method
In the transparent plastic bottle (high 12cm, diameter 6.5cm) of shell, calculated according to cocoon volume and bottle body product, every 400cm3Bottle volume theory
On can accommodate 1500 cocoons, but in order to prevent transport in collision cause cocoon to damage, appropriate cushion, i.e. buckwheat need to be filled in bottle
Or rice shell is to reducing collisions, under the premise of making full use of bottle space, 500-1000 cocoons of every bottle of placement are convenient, it is ensured that
The high viability of cocoon.Packaged beautiful Chrysopa cocoon can transport directly to field adaptability application, or be placed in 7-10 DEG C of term refrigeration storage
It deposits.
Further, beautiful Chrysopa adult uses box-packaged method the following steps are included: the beautiful Chrysopa adult newly to sprout wings, collection
In be collected into transparent vessel, be uniformly provided with 15-20 air hole on container lid, the ox-hide of decussation placed in container
Paper fills the ratio of 100-150 adults as isolation, according to each container 1000mL to pack adult.
Beneficial effect using the above scheme is: appropriate number of air hole is conducive to ventilation, guarantee beautiful Chrysopa at
The normal survival of worm, setting isolation are beneficial to prevent beautiful Chrysopa and kill each other, and suitably dress worm amount is beneficial to prevent crowded, make grass
Sandfly is freely movable, and can make full use of packaging space.
Each 1000mL can fill 100-150 adults, and when density is lower than 100 adults, space cannot be sufficiently sharp in bottle
With, when being higher than 150, adult density is excessive, and it is easy to cause adult wing to damage, flight performance decline, answering after reducing release
Use effect.Packaged beautiful Chrysopa adult can transport directly to field adaptability application.
The present invention also provides a kind of beautiful Chrysopa storage method, storage method includes term refrigeration storage and/or long-term diapause
Storage.
Beneficial effect using the above scheme is:, can when the product quantity of beautiful Chrysopa is significantly larger than practical application request
Extra beautiful Chrysopa is subjected to term refrigeration storage or long-term diapause storage according to actual needs in the form of cocoon.
Further, the condition of term refrigeration storage is 8-10 DEG C, longest Storage period 30-60 days.
Further, when term refrigeration stores, the beautiful Chrysopa cocoon cocoond 8 days is put into insect box, places ratio are as follows: is every
The quantity that the insect box of 800mL places cocoon is 2000-3000 head.
Use the above method has the beneficial effect that the beautiful Chrysopa by normal development is stored with the state term refrigeration of cocoon, can
It is on-demand, adult is turned into the rear beautiful Chrysopa energy short time under the conditions of refrigeration terminates to be transferred to normal development, is met regular
Interval continous way expands numerous production requirement, or lays in a large amount of beautiful Chrysopa products in a short time and carry out concentrating release application.
Further, it after the method for long-term diapause storage is the following steps are included: newly emerging adult starts oviposition, collects 24 hours
Interior new oviposition is placed in larva raising box, and 26 ± 1 DEG C, cultivate under the conditions of 16L:8D, RH70%, after egg hatching, raising children
Worm is relayed when larvae development to 1 latter stage in age and induces diapause under the conditions of 20 DEG C, 8L:16D, RH70%, daily basis
The appetite and developmental state of beautiful Chrysopa feed aphid in time, after beautiful Chrysopa mature larva starts to cocoon, collect new formed day by day
Cocoon, be first placed at 10 DEG C 2 days, transfer to 4-6 DEG C, humidity 60-70%, dark condition storage.
Further, when new oviposition is placed in larva raising box, according to every 6000cm3Raising box body accumulate and set ovum grain 80-
100 ratios place ovum grain.
Have the beneficial effect that inventor has found that the density of different ovum grains has pupa pick-up rate under study for action using the above method
It significantly affects, with the increase of larva raising density, the individual amount cocoond that can normally pupate is gradually reduced.Ovum granule density is excessively high
When, larva is killed each other seriously, and pupa pick-up rate reduces.Space utilization rate and pupa pick-up rate factor are comprehensively considered, using above-mentioned ratio
Example is optimal proportion, if feeding quantity is less, space utilization rate is lower, and feeding quantity increase will lead to overcrowding, makes children
Worm autotomy rate increases, and the cocoon number finally obtained also declines.
Long-term diapause storage method of the present invention can make beautiful Chrysopa cocoon safe storage 60-360 days or even 1 year or more,
Shelf life is set to improve 6-10 times, eclosion rate, can using this method still up to 82% or more after termination of diapause for the cocoon of storage
Storage period and the storage level for significantly improving beautiful Chrysopa product promote the merchandized handling of beautiful Chrysopa and are widely applied for a long time.
Detailed description of the invention
Fig. 1 is of the present invention for raising, storing the structure drawing of device of beautiful Chrysopa, in Fig. 1, portion representated by each label
Part is listed as follows: 1, box body；2, box cover, 3, folded filter paper item；4, gauze.
Fig. 2 is that numerous flow chart is expanded in the scale of beautiful Chrysopa of the present invention.
Specific embodiment
The principle and features of the present invention will be described below with reference to the accompanying drawings, and the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the invention.
The present invention provides a kind of pea tail reparing aphid and the extensive method for breeding of beautiful Chrysopa, as shown in Fig. 2, this method packet
It includes following steps: 1) aphid standardization batch production: pea tail reparing aphid is bred using semen viciae fabae seedling as host plant, every 3-5 days
Breed a batch；The condition of culture of aphid and semen viciae fabae seedling are as follows: 25 ± 1 DEG C of temperature, relative humidity RH60%-70%, the photoperiod
L:D=16:8 (daylight 16 hours, night is 8 hours dark), control light source are 40W fluorescent lamp, fluorescent lamp and bean seedlings top
Distance be 30-40cm.2) beautiful Chrysopa large-scale breeding: the condition of culture of beautiful Chrysopa is 26 ± 1 DEG C, 16L:8D, RH70 ±
5%, beautiful Chrysopa is raised by prey of pea tail reparing aphid, breeding process is divided into the collection of beautiful Chrysopa ovum and hatching, larva is concentrated to raise
Feeding, mature larva concentration pupates and collects beautiful Chrysopa cocoon, adult group breeding etc., can carry out according to actual needs field adaptability and answer
With；The concentration hatching of ovum is that beautiful Chrysopa is produced to ovum collecting interior for 24 hours in transparent plastic box, is placed in incubator and is incubated
Change.After larva raising and pupating refers to that egg hatching is larva, enough band aphid semen viciae fabae seedlings are added in raising box daily, until larva
It pupates and cocoons.Adult group breeding, which refers to, is put in dependent insect cage in-group raising, pangamy oviposition for beautiful Chrysopa cocoon concentration.This hair
Bright high-quality and efficient, easy economic, the standardization large-scale production throughout the year for realizing aphid and Chrysopa, can be used for preventing and treating a variety of aphids
Worm, aleyrodid, coccid, harmful mite and the agriculture and forestry injurious insects such as Lepidoptera ovum and low instar larvae, meet facilities vegetable, crop field, orchard and flower
The biological control demand of grass etc..
It is described further below by specific embodiment.
In embodiment, pea tail reparing aphid picks up from Yunnan Province, field, Kunming pea field.Beautiful Chrysopa picks up from Scientia Agricultura Sinica
Institute's Plant Protection Institute Langfang scientific base Cotton Fields.All can be the public obtain for repeating embodiment of the present invention.
The screening of the beautiful Chrysopa of embodiment 1 substitution prey
Influence of the 1.1 different Aphid Species to beautiful Chrysopa growth and development
26 ± 1 DEG C indoors, under the conditions of photoperiod 16L:8D (daylight 16 hours, night dark 8 hours), survey respectively
Determine the influence of pea tail reparing aphid, soybean aphid Aphis glycines and black peach aphid Myzus persicae to beautiful Chrysopa growth and development,
Filter out the Aphid Species of the beautiful Chrysopa of suitable vast propagation.
Experimental method are as follows: 26 DEG C, the first oviposition (in for 24 hours) of beautiful Chrysopa is collected under the conditions of 16L:8D, ovum grain is placed in bottom
Tiling has in the raising box of corrugation filter paper item, and every processing is repeated 3 times, 60 ovum of every repetition.Ovum is placed in above-mentioned experimental station manage bar
It is incubated under part, after egg hatching, adds enough aphid feedings daily, until larva cocoons, it is daily to collect cocoon and place it in training
It supports in ware (9 × 1.5cm).The cocoon collected under every treatment conditions dissection when 5 age in days, with electronic balance (Sartor ius,
BP211D, Germany) fresh weight (FM) that weighs naked prepupa, record respectively the beautiful Chrysopa of three kinds of aphids feeding from egg development to adult institute
The time needed and adult eclosion rate.Each processing is repeated 3 times, and 30 cocoons are at least observed in every repetition.
The analysis of 1.2 data
In experiment all data normality D ' Agostino&Pearson Omnibus Methods of Normality Test (α=
0.05) it tests.The comparison of beautiful Chrysopa prepupa fresh weight, development duration and adult eclosion rate uses One-Way ANOVA, difference
Significance test uses Tukey multiple comparison graph.All data analyses are in statistical analysis software GraphPad Prism V7.0
It completes.
1.3 experimental result
The results are shown in Table 1 for influence of the different Aphid Species to beautiful Chrysopa growth and development, as seen from table, feeds different aphids
The development duration of beautiful Chrysopa prepupa fresh weight, ovum to adult is had a significant impact, but adult eclosion rate is had no significant effect.Feed pea
The beautiful Chrysopa cocoon that beans repair tail aphid group is significantly higher than fed soy aphid group again, but and feeds black peach aphid group without significant difference；It is repaired with pea
Tail aphid feeding individual, development duration be significantly it is short, the death rate of larva and pupa is lower and adult eclosion rate is higher.Therefore,
Pea tail reparing aphid can be used as preferably substitution prey for expanding rich and flowery Chrysopa.
Influence of the different Aphid Species of table 1 to beautiful Chrysopa growth and development
| Aphid Species | Prepupa fresh weight (mg) | Ovum-Adult Development goes through phase (day) | Adult eclosion rate (%) |
| Pea tail reparing aphid | 12.46±0.07a | 24.75±0.97b | 90.1±3.44 |
| Soybean aphid | 11.91±0.22b | 25.18±0.93a | 86.1±6.91 |
| Black peach aphid | 12.35±0.09a | 25.13±1.21a | 84.4±8.85 |
The large-scaled propugation of 2 pea tail reparing aphid of embodiment
2.1 host plants are cultivated
It is screened, selects the semen viciae fabae that kind is natural king as the host plant for expanding numerous pea tail reparing aphid, the silkworm of the kind
Beans seed is full, germination percentage is high, emergence is neat, aphid is fond of, carries aphid amount height, is the good host plant for expanding numerous pea tail reparing aphid
Object.
Raising aphid on a large scale using semen viciae fabae seedling, specific step is as follows: 25 ± 1 DEG C indoors, photoperiod 16L:8D it is (white
Its illumination 16 hours, night are 8 hours dark) under the conditions of, sterilization treatment is put into 24 hole seedling-growing containers (30 × 20 × 10cm)
Vermiculite, vermiculite thickness about 2.5cm, vermiculite is flattened, and makes its filling slightly consolidation, is screened a big full Broad Bean Seeds and is uniformly sprinkling upon
On vermiculite, every basin is according to every 600cm2The ratio of 200-220 semen viciae fabaes of sowing carries out nursery, and evenly laid out on Broad Bean Seeds
The vermiculite (just not having Broad Bean Seeds to be advisable with vermiculite) of one thickness about 2cm.Seedling-growing container is placed in plastic pallet (34 × 26 × 3cm)
It inside pours into suitable quantity of water and vermiculite wetting uniformly (is watered unsuitable excessive, prevent seed decay), finally by seedling-growing container together with pallet one
It rises to be placed on multilayer feeding worm frame (1.5 × 0.5 × 3.0m) and cultivate, is poured on a small quantity as one sees fit before the budding of semen viciae fabae seedling according to vermiculite humidity
Water, to keep vermiculite surface layer is wet to be advisable.
2.2 aphids are inoculated with and expand numerous
25 ± 1 DEG C indoors, under the conditions of photoperiod 16L:8D, semen viciae fabae after planting about needs emerge for 4-6 days, semen viciae fabae after 7-8 days
It carries out connecing aphid (pea tail reparing aphid) for the first time when seedling plant height reaches 7-9cm, connecing aphid amount is every young plant 4-6 aphids, produces semen viciae fabae seedling
The raw Resistant reaction to aphid；If aphid amount is on the low side after connecing aphid for the first time, it may be selected to carry out the when 2-3 days after connecing aphid for the first time
Secondary to connect aphid, connecing aphid amount is every young plant 10-15 aphids；15 days or so, aphid can be covered with semen viciae fabae seedling stalk and leaf is positive and negative
Face, at this time the load aphid amount about 300-500 head of every plant of semen viciae fabae seedling, about 60000-70000 aphid of each seedling-growing container.The growth of semen viciae fabae seedling
During developing and connecing aphid, according to the wet degree of vermiculite in room temperature, humidity condition and seedling-growing container, poured in time to semen viciae fabae seedling
Water, in case water shortage influences the growth of semen viciae fabae seedling and aphid expansion is numerous.
2.3 aphids batch expands numerous
Every 3-5 days are a batch, repeat above-mentioned nursery, connect aphid, the numerous operation of aphid expansion, it may be assumed that first nursery 3 days laggard
It is numerous to realize that continuity batch expands with above-mentioned 2.1 and 2.2 steps, mentions to expand numerous natural enemy insect for row second batch nursery, subsequent operation
For sufficient stable aphid supply amount.
The scale of the beautiful Chrysopa of embodiment 3 expands numerous
Influence of 3.1 different temperatures to beautiful Chrysopa growth and development
Expand numerous preference temperature for the beautiful Chrysopa scale of determination, under the conditions of measuring photoperiod 16L:8D respectively, 20 DEG C of temperature, 24
DEG C, 26 DEG C, 30 DEG C to beautiful Chrysopa development duration, percentage of pupation, adult eclosion rate and cocoon weight influences.
Experimental method are as follows: 26 ± 1 DEG C, under the conditions of 16L:8D, collect the first oviposition (in for 24 hours) of beautiful Chrysopa, ovum grain is placed in
Bottom tiling has in the raising box of corrugation filter paper item, and every processing is repeated 3 times, 60 ovum of every repetition.Ovum is placed at above-mentioned temperature
It is incubated under the conditions of reason, after egg hatching, adds enough aphid feedings daily, until larva cocoons, it is daily to collect cocoon and set
In culture dish (9 × 1.5cm).Respectively record condition of different temperatures under beautiful Chrysopa from egg development to adult needed for the time, at
Worm eclosion rate, and the prepupa fresh weight for 5 ages in days of cocooing.Data processing method is the same as 1.2.
Table 2 the experimental results showed that, beautiful Chrysopa can complete growth and development between 20-30 DEG C, and temperature is pre- to beautiful Chrysopa
Pupa fresh weight, development duration and adult eclosion rate have a significant impact.It being increased with temperature, the development duration of beautiful Chrysopa significantly shortens,
But more than after optimum range, pre- pupal weight and adult eclosion rate decline temperature；The development duration of beautiful Chrysopa obviously prolongs under low temperature
Long, egg hatching rate reduces, and larval mortality increases, and the individual ratio that can be developed to adult reduces.In summary index is it is found that suitable
The temperature range of suitable beautiful Chrysopa growth and development, wherein 26 DEG C are convenient temperature, may be selected in the temperature between 26-30 DEG C
Raise beautiful Chrysopa on a large scale down.
Influence of the different temperatures to beautiful Chrysopa growth and development under the conditions of 2 16L:8D of table
Influence of the 3.2 different larva raising density to beautiful Chrysopa growth and development
For the best stocking density of the beautiful Chrysopa larva of determination, each raising box is respectively set, and (volume of raising box is
6000cm3) 40,60,80,100,120 five density of ovum are placed, observe the egg hatching of beautiful Chrysopa under the conditions of different stocking densities
The development duration of rate, pupa pick-up rate and larva, specifies influence of the stocking density to beautiful Chrysopa growth and development, and be preferably suitable for out is close
Spend range.
Experimental method are as follows: 26 ± 1 DEG C, under the conditions of 16L:8D, collect the first oviposition (in for 24 hours) of beautiful Chrysopa, ovum grain is placed in
Bottom tiling has in the raising box of corrugation filter paper item, places according to 40,60,80,100,120 ovum of every box, every processing repeats 3
It is secondary.Ovum is placed under above-mentioned temperature treatment conditions and is incubated for, after egg hatching, enough aphid feedings are added daily, until larva knot
Cocoon, it is daily to collect cocoon and place it in culture dish (9 × 1.5cm).The ovum of beautiful Chrysopa is incubated under the conditions of record different densities respectively
Rate, pupa pick-up rate.Data processing method is the same as 1.2.
Influence of the different stocking densities of table 3 to beautiful Chrysopa larva growth and development
The above results show under different stocking densities between the egg hatching rate of beautiful Chrysopa without significant difference, each treatment conditions
Lower 96% or more ovum can normal incubation.But different densities have a significant impact pupa pick-up rate, with larva raising density
Increase, the individual amount cocoond that can normally pupate is gradually reduced.When stocking density is lower than 100/box, pupa pick-up rate between each processing
Difference is not significant；But when the stocking density of every box is more than 100, larva is killed each other seriously, and pupa pick-up rate is less than 75%.It is comprehensive
Considering space utilization rate and pupa pick-up rate factor, it is preferable density that every box, which raises 80-100 larvas, if feeding quantity is less,
Space utilization rate is lower, feeding quantity increase will lead to it is overcrowding, make larva autotomy rate increase, the cocoon number finally obtained also under
Drop.
The management and hatching of 3.3 beautiful Chrysopa ovum
Under the conditions of 26 ± 1 DEG C indoor, 16L:8D, adult is placed in dependent insect cage (60 × 60 × 60cm) and uses pea tail reparing aphid
Even generation raising establishes and stablizes population.From new emerging adult lays eggs day, daily timed collection is newly laid eggs, and ovum grain is placed in bottom
Be covered with folded filter paper item (long 30cm × wide 1.5cm, prevent Chrysopa larva from killing each other) raising box (30cm × 20cm ×
In 10cm).As shown in Figure 1, raising box includes box body 1 and box cover 2；1 top opening of box body, box body 1 are internally provided with folding filter
Paper slip 3；Box cover 2 covers at 1 top of box body；A rectangular through holes (20cm × 12cm) is cut off among raising box box cover 2 and with 60
The gauze 4 of mesh nylon material covers, and the gauze 4 has air hole, guarantees air circulation.Every box places 80-100 ovum, will
Above-mentioned raising box, which is placed in concentrate in incubator to ovum, hatches, and incubator condition setting is 26 ± 1 DEG C, 16L:8D, RH70 ± 5%.
It is 1 instar larvae that Chrysopa ovum, which is largely hatched, generally after 4-6 days, from hatching day to 1 instar larvae, the daily enough aphids of timing feeding, just
Larvae appetite is small, and average every larva feeds 30-80 aphids daily can meet growth and development demand.
3.4 beautiful Chrysopa larva group breedings
With the growth and development of 1 instar larvae, appetite is gradually increased, and need to increase aphid according to the feeding situation of proxima luce (prox. luc) larva
Scale of feeding.General 2 instar larvae daily every averagely feeds 100-150 aphids, and 3 instar larvaes daily every averagely feeds 120-
200 larvas.2-3 instar larvae appetite explodes, and after the enough band aphid semen viciae fabae seedlings of daily iron supplement, need to clear up the plant in raising box in time
The residues such as residual branch, aphid corpse, guarantee the cleaning of feeding environment, prevent the excessively high generation mould of humidity in box.In above-mentioned raising
Under the conditions of, being developed to 3 age mature larvas from 1 instar larvae about needs 8-10 days.
3.5 mature larvas concentration is pupated
In dark brown to grey black, feeding is reduced and slow in action 3 age mature larvas, after obtaining enough nutrition, that is, is sought
Look for shelter to cocoon, generally can in the groove of folded filter paper item 3, withered branches and leaves and raise the bottom of box and cocoon, 3 ages
Larva first passes through the prepupa stage in cocoon and is developed to pupa again, judges that the standard pupated is when the visible black husking in cocoon end
When, prepupa develops pupa, which about needs 5-8 days or so.After mature larva starts to cocoon, day by day collects and newly to be formed
Cocoon is relayed in new raising box, and is marked and collected date and quantity, for product packaging or expands numerous use.
3.6 adult eclosions and ovum grain are collected
Under the conditions of 26 ± 1 DEG C indoor, 16L:8D, the beautiful Chrysopa cocoon newly collected is placed in dependent insect cage (60 × 60 × 60cm)
Concentration emergence is carried out, every cage places 400-500 cocoons.Beautiful Chrysopa starts to turn into adult after pupating for 8-10 days or so, new to sprout wings
Adult about needs can lay eggs within 4-6 days after abundant feeding.Adult has phototaxis, and close to sources is leaned in general happiness at the top of dependent insect cage
Place's aggregation.Oviposition medium is placed in rearging cage and concentrates oviposition for beautiful Chrysopa, while being placed basin band aphid semen viciae fabae seedling and being supplemented for adult
Nutrition is inhabited and is laid eggs, and institute's outdoor rearing of tussah silkworm bean seedlings are 10-12 age in days, and carrying aphid amount should not be excessive, carries aphid 50-80 head with every young plant
It is advisable, Chrysopa does not like to lay eggs on it when load aphid amount is excessive.After collecting a large amount of ovum grains on medium of laying eggs in rearging cage, by its turn
It moves to egg hatching in new rearging cage or collects production ovum card for ovum grain, while replacing new oviposition medium and being produced for adult
Ovum.
According to production requirement, under the premise of guaranteeing that beautiful Chrysopa routinely expands numerous, the beautiful Chrysopa ovum of production, each instar larvae,
Prevention and treatment after cocoon and adult can be packaged, for a variety of aphids in field, harmful mite, thrips, coccid and lepidoptera pest etc..When
When the product quantity of beautiful Chrysopa is significantly larger than practical application request, extra beautiful Chrysopa can be subjected to term refrigeration in the form of cocoon
Storage or long-term diapause storage, discharge application again when needed.
In conclusion the invention has the following advantages that
1. preferred substitution prey pea tail reparing aphid and its most suitable host plant are developed with the pea tail reparing aphid that semen viciae fabae seedling is bred
Neatly, quantity is big, the breeding cycle is short, at low cost, realizes the standardized production of aphid.
2. aphid and beautiful Chrysopa can be expanded numerous in the anniversary, production is not subject to seasonal restrictions, and can meet annual application demand, sufficiently
Numerous worm facility and space is utilized, has saved cost.
Aphid provided by the present invention, Chrysopa artificial fecundation method realize the standardization large-scale production of aphid, Chrysopa,
Multiplication technique standard criterion, economical and efficient realize the high-quality and efficient long-term production of beautiful Chrysopa, promote the commercialization of beautiful Chrysopa
Production and extensive use.
Inventor combines ecology, nutritional development, physiology and its predation pest controlling merit rating of beautiful Chrysopa etc., carries out
The vast propagation technical research of beautiful Chrysopa.It cultivates, connect around aphid and the screening of numerous aphid host plant, host plant standardization
The key technologies such as aphid key opportunity, the best stocking density of Chrysopa, rearing conditions through test of many times and are improved, are had developed a set of
Efficiently, easy, economic beautiful Chrysopa multiplication technique, substantially increases the yield of Chrysopa, realizes the anniversary scale of Chrysopa product
It is numerous to change expansion, the biological control demand of a variety of agriculture and forestry injurious insects based on aphid can be met, the production to the natural enemy insects such as Chrysopa are promoted
Industry metaplasia produces and large area release application is of great significance.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of method for raising beautiful Chrysopa using pea tail reparing aphid, which is characterized in that raised using pea tail reparing aphid as prey beautiful
Chrysopa；Beautiful Chrysopa method for breeding is the following steps are included: beautiful Chrysopa ovum collecting and hatching, beautiful Chrysopa larva group breeding, aging children
Worm, which is concentrated, to pupate to form beautiful Chrysopa cocoon, beautiful Chrysopa adult eclosion and raising.
2. the method for raising beautiful Chrysopa using pea tail reparing aphid according to claim 1, which is characterized in that beautiful Chrysopa ovum collecting
With the method for hatching the following steps are included: 26 ± 1 DEG C, under the conditions of 16L:8D, adult pea tail reparing aphid even generation raising is established
Stablize population；From new emerging adult lays eggs day, daily timed collection is newly laid eggs, and ovum grain is placed in bottom and is covered with folded filter paper item
Raising box box body in, according to every 6000cm3Raising box body accumulate and set the ratio of 80-100 ovum and place beautiful Chrysopa ovum, raise
The box cover for supporting box is equipped with through-hole and is covered with nylon gauze, and nylon gauze has air hole；Later hatching culture, condition be 26 ±
1 DEG C, 16L:8D, RH70 ± 5%；It is 1 instar larvae that Chrysopa ovum, which is largely hatched, generally after 4-5 days, from hatching day to 1 instar larvae, often
Day enough aphids of timing feeding, average every larva feed 30-80 aphids daily.
3. the method for raising beautiful Chrysopa using pea tail reparing aphid according to claim 1, which is characterized in that beautiful Chrysopa larva group
The method of body raising is the following steps are included: 2 instar larvaes daily every averagely feeds 100-150 aphids, 3 instar larvaes every head daily
Averagely feed 120-200 aphids；And guarantee feeding environment cleaning.
4. the method for raising beautiful Chrysopa using pea tail reparing aphid according to claim 1, which is characterized in that mature larva is concentrated
It pupates to form the method for beautiful Chrysopa cocoon the following steps are included: guaranteeing nutrition, first passes through the prepupa stage in cocoon to 3 instar larvaes and send out again
It educates to pupa, judges that the standard pupated is when the visible black husking in cocoon end, prepupa develops pupa, which needs 5-8
It；After mature larva starts to cocoon, the beautiful Chrysopa cocoon newly formed is collected day by day, is relayed in new raising box, and mark receipts
Collect date and quantity, for product packaging or expands numerous use.
5. any one of -4 method for raising beautiful Chrysopa using pea tail reparing aphid according to claim 1, which is characterized in that beautiful grass
Sandfly adult eclosion and the method for raising the following steps are included: 26 ± 1 DEG C, under the conditions of 16L:8D, the beautiful Chrysopa cocoon newly collected is set
Concentration emergence is carried out in dependent insect cage, according to every 216000cm3The ratio that the dependent insect cage of volume places 400-500 cocoons is placed beautiful
Chrysopa cocoon；Beautiful Chrysopa starts to turn into adult after pupating for 8-10 days, and new emerging adult needs can produce within 4-6 days after abundant feeding
Ovum；Band aphid semen viciae fabae seedling is placed in rearging cage for Adult worms producting eggs, institute's outdoor rearing of tussah silkworm bean seedlings are 10-12 age in days, carry aphid 50-80 with every young plant
Head transfers them to egg hatching in new rearging cage after collecting enough beautiful Chrysopa ovum or collects production ovum for ovum grain
Card, while new band aphid semen viciae fabae seedling is replaced for Adult worms producting eggs.
6. any one of -4 method for raising beautiful Chrysopa using pea tail reparing aphid according to claim 1, which is characterized in that pea
The method for breeding of tail aphid is repaired the following steps are included: (1) selects semen viciae fabae to be educated as the host plant of the numerous pea tail reparing aphid of expansion
Seedling；(2) aphid is inoculated with and expands numerous.
7. the method for raising beautiful Chrysopa using pea tail reparing aphid according to claim 6, which is characterized in that in step (1), choosing
Selecting kind is the semen viciae fabae of natural king as host plant, and the method for nursery is the following steps are included: 25 ± 1 DEG C, photoperiod 16L:8D
Under the conditions of, it is put into the vermiculite of sterilization treatment into seedling-growing container, screens a big full Broad Bean Seeds and is uniformly sprinkling upon on vermiculite, according to
Every 600cm2The ratio nursery of 200-220 semen viciae fabaes, and evenly laid out one layer of vermiculite on Broad Bean Seeds are sowed, is not had just with vermiculite
Crossing Broad Bean Seeds is advisable；Watering is cultivated with keeping vermiculite surface layer is wet to be advisable.
8. the method for raising beautiful Chrysopa using pea tail reparing aphid according to claim 6, which is characterized in that step (2) aphid
It is inoculated with and expands numerous the following steps are included: under the conditions of 25 ± 1 DEG C, photoperiod 16L:8D, semen viciae fabae seedling strain after semen viciae fabae after planting 7-8 days
It carries out connecing aphid for the first time when up to 7-9cm, connecing aphid amount is every young plant 4-6 aphids；If aphid amount is on the low side, after connecing aphid for the first time
Selection in 2-3 days is connect aphid for the second time, and connecing aphid amount is every young plant 10-15 aphids；15-18 days, aphid was covered with semen viciae fabae seedling stalk
And leaf front and back sides, the load aphid amount 300-500 head of every plant of semen viciae fabae seedling at this time, each seedling-growing container 60000-70000 aphids, the phase
Between, watering.
9. the method for raising beautiful Chrysopa using pea tail reparing aphid according to claim 6, which is characterized in that further include step
(3) aphid batch expands numerous step, operating method are as follows: every 3-5 days are a batch, repeat above-mentioned nursery, connect aphid, the numerous behaviour of aphid expansion
Make.
10. a kind of for raising the device of beautiful Chrysopa, which is characterized in that including box body and box cover；Box body top opening, in box body
Portion is provided with folded filter paper item；Box cover covers at the top of box body, and box cover is provided with through-hole, and yarn is covered at lead to the hole site
Net, the gauze have air hole.
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