CN111758672A - Group breeding method for feeding spodoptera frugiperda larvae with young corn shoots - Google Patents
Group breeding method for feeding spodoptera frugiperda larvae with young corn shoots Download PDFInfo
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- CN111758672A CN111758672A CN202010685276.3A CN202010685276A CN111758672A CN 111758672 A CN111758672 A CN 111758672A CN 202010685276 A CN202010685276 A CN 202010685276A CN 111758672 A CN111758672 A CN 111758672A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a group breeding method for feeding spodoptera frugiperda larvae by using young corn shoots, which is characterized in that fine sand is paved at the bottom of a breeding box, different tissue parts of the young corn shoots, such as young corn shoot bracts, young corn shoot cores, young corn shoots and the like, are taken as food materials, and are alternately arranged on the surface of a sand layer to form a multi-layer stacked structure for breeding the spodoptera frugiperda larvae, and pupae is screened from sand and eggs are collected from adults to finish the growth generation of the spodoptera frugiperda. Its operation flow is simple, safety and reliability, make full use of the characteristics of the different component parts of young maize as the foodstuff, built the surface in narrow and small raising box and piled up, form wide range stack structure, keep apart grassland spodoptera frugiperda larva can kill the action that produces of mutual contact effectively, and utilize sand dry raising box class internal environment and pile to bury simultaneously and form suitable humidity and do benefit to the function that the grassland spodoptera pupate, the pupa of collecting is concentrated in the later stage of being convenient for to pupate in the sand to old and mature larva, formed the group ization raising method, and save the manual work.
Description
Technical Field
The invention belongs to the field of biotechnology of agriculture and forestry, relates to a method for artificially feeding spodoptera frugiperda, and particularly relates to a method for feeding spodoptera frugiperda larvae with young corn shoots for group feeding.
Background
Corn is a gramineous crop, belongs to an annual herbaceous plant, is a food resource on which human beings live, is also an important cereal crop in China, and has the total yield second to that of rice. In recent years, corn has been developed into important food, feed and industrial raw crop in China. The development scale of corn production becomes the important factor for determining the development of the animal husbandry and the corn processing industry in left and right China, and is an important component of national food safety. Spodoptera frugiperda, commonly known as Fall armyworm (Fall armyworm), belongs to the genus Trichoplusia in the family Spodoptera, native to the tropical and subtropical regions of the America, and is a major dangerous migratory insect pest that seriously threatens agricultural production. In 2016, 1 month, the fall armyworm inhabiting the continental america was brought by commercial and trade shipments to nigeria on the west coast of africa, and subsequently only 2 years of time was spent panning 44 countries and regions in the south of sahara, which had a great impact on food safety in africa. Since 7 months in 2018, Spodoptera frugiperda migrated across the sea into also and India, gradually spreading deep throughout southeast Asian countries.
In 11 days in 1 month and 11 months in 2019, Spodoptera frugiperda is found as a pest corn for the first time in Jiangcheng county of Puer city in Yunnan province and is gradually spread to 26 provinces in China at present, and the corn generating area in 2019 is about 106.5 kilohm2And causes harm to other crops such as 14 crops of sugarcane, sorghum, millet, wheat and the like. In the rural area of agriculture, the emergence situation of the spodoptera frugiperda is strictly concerned when the spodoptera frugiperda epidemic situation is outbreak in Asian countries and regions, and the monitoring, prevention and control work of the spodoptera frugiperda is highly regarded. The spodoptera frugiperda monitoring and early warning work is arranged and deployed in 12 months in 2018, the national agriculture technology center issues ' notification about the work of preventing spodoptera frugiperda from invading harm ' (Chinese plant protection and journal information, 2019) in 2019 in 1 month, and ' national spodoptera frugiperda prevention and control work is formally issued in 6 months in 2019 by the rural ministry of agricultureThe scheme carefully guides and deploys the Spodoptera frugiperda prevention and control target tasks, monitoring and prevention and control measures and other aspects in China, ensures that the Spodoptera frugiperda is not damaged by migration on a large scale in a main corn production area, ensures that the corns are in a continuous disaster with a small area, and furthest lightens the overall target tasks of disaster loss.
With the continuous deepening of spodoptera frugiperda scientific research and the development of prevention and control technology, a large number of consistently developed and physiologically-drawn insect sources are needed and are limited by seasons and growth cycles of host plants, especially, the spodoptera frugiperda larvae can be killed each other or bite pupae frequently at higher density, and the situation that the larvae bite pupae is caused, so that the feeding mode of a single larva is time-consuming and labor-consuming, and the residual foodstuff is also wasted, therefore, an optimized feeding method and a flow are urgently needed to group-feed the spodoptera frugiperda larvae, and a large number of physiologically-drawn insect sources are provided for carrying out scientific researches on spodoptera frugiperda such as biology, ecology, physiology and the like and carrying out natural enemy parasitic wasp expansion in future.
Disclosure of Invention
The invention aims to provide a groupware feeding method for feeding spodoptera frugiperda larvae by using young corn shoots, which has the advantages of simple operation process, safety and reliability, fully utilizes the characteristics that different components of the young corn shoots are used as foodstuff, builds surface accumulation in a narrow feeding box, forms a wide stacking structure, effectively isolates the killing behavior generated by the mutual contact of the spodoptera frugiperda larvae, utilizes the environment in the sand drying feeding box to form suitable humidity beneficial to the pupation of the spodoptera frugiperda and provides a pupation place, and pupation of old and mature larvae in sand is convenient for later-stage centralized collection of pupation, thereby forming a groupware feeding method and saving labor; provides a large number of uniformly developed insect sources for testing for further expanding the research and application of the techniques such as the biological ecology, physiology, toxicology, biological control and the like of the spodoptera frugiperda in the future.
In order to achieve the purpose, the technical scheme of the invention is as follows: provides a grouinization feeding method for feeding spodoptera frugiperda larvae with young corn shoots, which comprises the following steps:
(1) preparing a Spodoptera frugiperda larva breeding box:
(1-1) paving sand at the bottom of the box;
(1-2) stacking the corn shoot bracts, the corn shoot cores and the corn stigma on the upper layer of the sand to form a multilayer three-dimensional space structure;
(1-3) placing corn stigma and young baby corn cores on the uppermost layer, and using the young baby corn stalks for feeding Spodoptera frugiperda of the low age;
(2) the larva breeding process:
(2-1) placing corn seedlings or kraft paper with spodoptera frugiperda egg masses on the corn silk and young baby corn shoot cores on the surface layer, and taking the young baby corn silk and young baby corn shoot cores from hatched larvae;
(2-2) periodically replacing fresh baby corn after the larvae grow to 4 th instar, cleaning insect dung, and simultaneously keeping a stacked structure among baby corn bracts, baby corn cores and corn stigma so as to avoid mutual killing behavior caused by mutual contact of the larvae after 4 th instar and enable the pupated aged larvae to enter sand to pupate;
(3) collecting pupae, raising imagoes and storing eggs:
(3-1) after the aged larvae pupate in sand, screening out the aged larvae from the sand by using a sample separation screen, putting the aged larvae into a glass bottle with a paper towel at the bottom pad of the glass bottle, tying a nylon net on the bottle mouth by using a rubber band, and standing at room temperature until pupae eclosion;
(3-2) putting the eclosion imagoes into an insect raising cage, and placing corn seedlings or kraft paper with edges torn into strips for the imagoes to lay eggs;
(3-3) replacing cotton balls and collecting egg blocks every day, wherein the relative humidity in a room where the insect cage is located is not lower than 70%;
(3-4) putting the collected spodoptera frugiperda egg blocks into a refrigerator with the temperature of 5-10 ℃ for storage for 1-5 days, and taking out the spodoptera frugiperda egg blocks and putting the spodoptera frugiperda egg blocks into a feeding box when the spodoptera frugiperda egg blocks are used.
Further, in the step (1-1), a ventilation net opening which occupies an area of the box cover and is welded by an iron wire net not less than 1/2 is further arranged on the box cover, sand in the box is paved to be 2-4 cm thick, and the sand is sterilized by dry heating at 120 ℃ for 2 h.
Further, in the step (2-1), a paper sheet, preferably printing paper, for preventing larvae from escaping is placed under the cover of the box.
Furthermore, in the step (2), after the dispersed larvae grow to 4 years old after eating the corn shoot cores and the bracts, the larvae are timely separated from the rearing boxes for treatment when the population density is more than 5.5 heads/L.
Further, in the step (2-2), fresh young corn shoots are replaced for 2-5 days in the breeding process.
Further, in the step (3-1), the sample separating sieve is 8-15 meshes, and the nylon net is 100-300 meshes.
Further, in the step (3-2), eclosion adults are put into an insect cage of a nylon mesh cloth of 100-200 meshes, corn seedlings or kraft paper with edges torn into strips are placed for spawning, and cotton balls dipped with 10% of honey water are hung for supplementing nutrition.
Further, in the step (3-4), the collected spodoptera frugiperda egg blocks are stored in a refrigerator at 5 ℃ for 1-5 days.
Furthermore, the young corn shoots are fruits which are picked early for harvesting large and full corns, or are picked early for specially producing the young corn shoots and cannot be picked all first until the corns grow.
Furthermore, the indoor temperature of the feeding box and the insect cage is 20-30 ℃, and the photoperiod L: D is 12-16 h: 12-8 h.
The sand in the box body can absorb the overhigh humidity caused by the excreted insect feces of the spodoptera frugiperda larvae after eating the corn shoots and the vapor exhaled by the stomata of the body wall of the middle-aged and old larvae, and the old larvae can pupate in the box body.
Furthermore, the indoor humidity is not enough, and a humidifier can be placed near the insect cage to improve the humidity of the local space.
Furthermore, the breeding box and the glass bottle are scalded or soaked by boiled water to eliminate the pollution of microorganisms.
The invention relates to a group breeding method for feeding spodoptera frugiperda larvae by using young corn shoots, which has the following beneficial effects:
1. the green corn earworm feed disclosed by the invention fully utilizes the bamboo shoot core, the bract, the corn stigma and the like of the young corn earworm, can be used as foodstuff to feed spodoptera frugiperda larvae, and can also utilize the cylindrical supporting effect of the bamboo shoot core to separate different components to form a stacked structure with a wide surface area, so that more space is created for the spodoptera frugiperda larvae, the phenomenon that feeding efficiency is reduced because the larvae can be killed after being contacted with one another is avoided, and large-scale feeding is realized.
2. The invention utilizes the advantages of wide source range of the young corn, easy long-term cold storage and the like, and avoids the defects that the corn leaves are easy to rot, lose water and need to be frequently replaced in the process of feeding the spodoptera frugiperda. And the young corn shoots are used as commodities, have a plurality of market supply channels, are convenient for logistics transportation and have remarkable advantages.
3. The sand layer is used as a pupation and pupation storage place for Spodoptera frugiperda, so that the humidity in the breeding box is reduced, pathogenic microorganisms are not easy to generate, the larvae can be isolated from pre-pupation and can be killed and eaten, meanwhile, the concentrated collection of pupation through a sand sieving mode is facilitated, the operation process is simple, and the method is reliable.
4. The method for breeding Spodoptera frugiperda in a group manner is formed by the whole step flow, large-scale subculture of Spodoptera frugiperda can be realized, breeding food is easy to obtain, and a physiological striking insect source is provided for scientific researches on biology, ecology, physiology and the like of Spodoptera frugiperda and propagation of natural enemy parasitic wasps in future.
Detailed Description
Example 1
The invention relates to a group breeding method for feeding spodoptera frugiperda larvae with young corn shoots, which comprises the following steps:
(1) preparing a Spodoptera frugiperda larva breeding box:
a. preparing a sterilized feeding box with the size of 40 multiplied by 28 multiplied by 16cm, wherein the box cover is provided with an opening with the length of 25cm and the width of 15cm, a wire mesh with 120 meshes is welded at the opening, and sand with the thickness of 2cm and which is sterilized by dry heat at the high temperature of 120 ℃ for 2 hours is laid at the bottom of the box;
b. taking a small amount of fresh corn spears, dispersing the fresh corn spears into corn spear bracts, corn spear cores and corn stigma, mutually spacing the corn spear bracts, the corn spear cores and the corn stigma by utilizing the cylindrical supporting effect of the corn spear cores, stacking the corn spear bracts, the corn spear cores, the corn stigma and the like on sand (for example, the corn spear cores, the corn spear bracts, the corn spear cores, the corn stigma and the like are arranged from bottom to top), and breaking off the slightly larger corn spear cores to form a stacking structure with a wide surface;
c. corn silk and tender bamboo shoot core are put in the uppermost layer and are used for feeding by the low-age larvae of Spodoptera frugiperda.
(2) The larva breeding process:
a. putting kraft paper containing 150 spodoptera littoralis eggs on corn stigma and young baby corn cores on the surface layer, feeding the young baby corn stigma with the hatched larvae, and adding a paper sheet under the box cover to prevent the larvae from escaping;
b. after the dispersed larvae eat the corn bamboo shoot cores, the corn bamboo shoot silks and the bracts and grow to 4 years old, when the insect population density is 5.5 heads/L, timely carrying out separation breeding box treatment;
c. in the breeding process, fresh young corn shoots are replaced for 3 days, insect dung is cleaned, meanwhile, a stacked structure between young corn shoots cores and bracts (or corn leaves) is kept, mutual killing caused by mutual contact of larvae after 4 years old is avoided, and pupated aged larvae enter sand to pupate;
(3) collecting pupae, raising imagoes and storing eggs:
a. after the aged larvae pupate in sand, screening out the aged larvae from the sand by using a 10-mesh sample separation screen, putting the aged larvae into a glass bottle with a paper towel at the bottom pad of the glass bottle, tying a nylon net with 100 meshes at the bottle mouth by using a rubber band, and standing at room temperature until pupae eclosion;
b. putting the eclosion imagoes into an insect cage with the thickness of 30 multiplied by 30cm and made of a nylon mesh cloth with the size of 100 meshes, placing corn seedlings for the spawning of the corn seedlings, and hanging cotton balls dipped with 10% of honey water for the nutrition supplement of the cotton balls;
c. cotton balls are replaced and egg blocks are collected every day, and the indoor relative humidity of the insect cage is about 80%, so that eclosion and egg laying of spodoptera frugiperda adults are facilitated in the indoor environment;
d. and (3) storing the collected spodoptera frugiperda egg blocks in a refrigerator at 5 ℃ for 2 days, and taking out the collected spodoptera frugiperda egg blocks when the spodoptera frugiperda egg blocks are used, and placing the spodoptera frugiperda egg blocks in a feeding box.
Example 2
The invention relates to a group breeding method for feeding spodoptera frugiperda larvae with young corn shoots, which comprises the following steps:
(1) preparing a Spodoptera frugiperda larva breeding box:
a. preparing a sterilized feeding box with the size of 40 multiplied by 28 multiplied by 16cm, wherein the box cover is provided with an opening with the length of 25cm and the width of 15cm, a wire mesh with 120 meshes is welded at the opening, and sand with the thickness of 4cm and which is sterilized by dry heat at high temperature of 120 ℃ for 2 hours is laid at the inner bottom of the box;
b. taking a small amount of fresh corn bamboo shoots, dispersing the fresh corn bamboo shoots into corn bamboo shoot bracts, corn bamboo shoot cores, corn stigma and other parts, mutually spacing the corn bamboo shoot bracts, the corn bamboo shoot cores and the corn stigma by utilizing the cylindrical supporting effect of the corn bamboo shoot cores, stacking the corn bamboo shoot bracts, the corn bamboo shoot cores, the corn stigma, the corn bamboo shoot cores, the corn bamboo shoot bracts and the like from bottom to top on sand, breaking off the corn bamboo shoot cores slightly, and forming a stacking structure with a wide surface;
c. corn silk and tender bamboo shoot core are put in the uppermost layer and are used for feeding by the low-age larvae of Spodoptera frugiperda.
(2) The larva breeding process:
a. putting kraft paper containing 150 spodoptera littoralis eggs on corn stigma and young baby corn cores on the surface layer, feeding the young baby corn stigma with the hatched larvae, and adding a paper sheet under the box cover to prevent the larvae from escaping;
b. after the dispersed larvae eat corn shoot cores and bracts and develop to 4 years old, when the population density is 5.5 heads/L, timely separating and feeding the larvae into a box for treatment;
c. in the breeding process, fresh young corn shoots are replaced and cleaned for 5 days, meanwhile, a stacked structure between young corn shoots cores and bracts (or corn leaves) is kept, mutual killing caused by mutual contact of larvae after 4 years old is avoided, and pupated aged larvae enter sand to pupate;
(3) collecting pupae, raising imagoes and storing eggs:
a. after the aged larvae pupate in sand, screening out the aged larvae from the sand by using a 10-mesh sample separation screen, putting the aged larvae into a glass bottle with a paper towel at the bottom pad of the glass bottle, tying a nylon net with 300 meshes at the bottle mouth by using a rubber band, and standing at room temperature until pupae eclosion;
b. putting eclosion imagoes into an insect cage made of 150-mesh nylon mesh cloth, placing kraft paper with the edge torn into a strip shape for spawning, and hanging cotton balls dipped with 10% honey water for supplementing nutrition;
c. replacing cotton balls and collecting egg blocks every day, wherein the indoor relative humidity of the insect cage is 90%, so that eclosion and egg laying of the spodoptera frugiperda adults are facilitated in the indoor environment;
d. and (3) storing the collected spodoptera frugiperda egg blocks in a refrigerator at 10 ℃ for 5 days, and taking out the collected spodoptera frugiperda egg blocks and placing the spodoptera frugiperda egg blocks in a feeding box when the spodoptera frugiperda egg blocks are used.
Example 3
The invention relates to a group breeding method for feeding spodoptera frugiperda larvae with young corn shoots, which comprises the following steps:
(1) preparing a Spodoptera frugiperda larva breeding box:
a. preparing a sterilized feeding box with the size of 40 multiplied by 28 multiplied by 16cm, wherein the box cover is provided with an opening with the length of 25cm and the width of 15cm, a wire mesh with 120 meshes is welded at the opening, and sand with the thickness of 3cm and which is sterilized by dry heat at high temperature of 120 ℃ for 3 hours is laid at the inner bottom of the box;
b. taking a small amount of fresh corn shoots, dispersing the fresh corn shoots into corn shoot bracts, corn shoot cores, corn stigma and other parts, mutually spacing the corn shoot bracts, the corn shoot cores and the corn stigma by utilizing the cylindrical supporting effect of the corn shoot cores, and stacking the corn shoot bracts, the corn shoot cores and the corn stigma on sand (such as the corn shoot bracts, the corn shoot cores, the corn stigma and the like from bottom to top), wherein the slightly larger corn shoot cores can be broken off by hand, so that a stacking structure with a wide surface is formed;
c. corn silk and tender bamboo shoot core are put in the uppermost layer and are used for feeding by the low-age larvae of Spodoptera frugiperda.
(2) The larva breeding process:
a. putting kraft paper containing 200 spodoptera littoralis eggs on corn stigma and young baby corn cores on the surface layer, feeding the young baby corn stigma with the hatched larvae, and adding a paper sheet under the box cover to prevent the larvae from escaping;
b. after the dispersed larvae eat corn shoot cores and bracts and develop to 4 years old, when the population density is 5.5 heads/L, timely separating and feeding the larvae into a box for treatment;
c. in the breeding process, fresh young corn shoots are replaced for 4 days, insect dung is cleaned, meanwhile, a stacked structure between young corn shoots cores and bracts (or corn leaves) is kept, mutual killing caused by mutual contact of larvae after 4 years old is avoided, and pupated aged larvae enter sand to pupate;
(3) collecting pupae, raising imagoes and storing eggs:
a. after the aged larvae pupate in sand, screening out the aged larvae from the sand by using a 10-mesh sample separation screen, putting the aged larvae into a glass bottle with a paper towel at the bottom pad of the glass bottle, tying a nylon net with 200 meshes at the bottle mouth by using a rubber band, and standing at room temperature until pupae eclosion;
b. putting eclosion imagoes into an insect cage made of 200-mesh nylon mesh cloth, placing kraft paper with the edge torn into a strip shape for spawning, and hanging cotton balls dipped with 10% honey water for supplementing nutrition;
c. replacing cotton balls and collecting egg blocks every day, wherein the indoor relative humidity of the insect cage is 70%, so that eclosion and egg laying of the spodoptera frugiperda imagoes are facilitated in the indoor environment;
d. and (3) storing the collected spodoptera frugiperda egg blocks in a refrigerator at 5 ℃ for 3 days, and taking out the collected spodoptera frugiperda egg blocks when in use and placing the spodoptera frugiperda egg blocks in a feeding box.
Control group 1
The procedure was the same as in example 1 of the present invention except that sand was not laid on the bottom of the insect-raising box.
Control group 2
The procedure was the same as in example 1 except that the young corn shoots were processed differently (after the young corn shoots were merely broken apart, laid flat, not stacked, and raised).
Comparison results
Calculated using the following formula:
the hatching rate (number of newly hatched larvae/total number of eggs tested) × 100%
The survival rate of the larva is (the number of the aged larva/the total number of the tested larva) multiplied by 100 percent
The pupation rate is (pupation number/tested larva number) x 100%
The eclosion rate is (eclosion insect number/pupa number) multiplied by 100 percent
TABLE 1 comparison of biological parameters of different herding feeding regimes for Spodoptera frugiperda
| Feeding mode | Egg hatchability/% | Survival rate of larvae/%) | Pupation Rate/% | Eclosion Rate/% |
| Inventive example 1 | 95.24±0.58a | 61.94±3.37a | 94.25±1.78a | 87.59±2.68a |
| Inventive example 2 | 94.35±0.65a | 59.92±3.45a | 92.91±1.34a | 85.49±1.46a |
| Inventive example 3 | 94.47±0.46a | 63.15±2.43a | 95.37±2.36a | 83.53±2.45a |
| ControlGroup 1 | 93.67±0.88a | 25.54±4.33b | 69.02±4.35b | 63.96±1.14b |
| Control group 2 | 94.00±0.36a | 29.67±3.39b | 95.12±1.53a | 85.52±1.12a |
As can be seen from table 1: using the method of the present invention (examples 1-3), i.e., a breeding method using young corn shoots as a foodstuff, adjusting humidity of an insect rearing box with sand and providing a pupation place, the egg hatchability was 95.24%, 94.35% and 94.47%, respectively; larva survival rates were 61.94%, 59.92% and 63.15%, respectively; pupation rates are 94.25%, 92.91% and 95.37% respectively; the emergence rates were 87.59%, 85.49% and 83.53%, respectively, and the larva survival rate, pupation rate and emergence rate were significantly higher than those of control group 1 (no sand as the base). Particularly, in the aspect of larva survival rate, the control group 1 is only about 25.54% and is far lower than that of the embodiment 1-3, which shows that the breeding mode that sand is laid at the bottom of the insect box and the young corn shoots are added (the young corn shoots are broken open to enable the young corn shoot cores and the young corn shoot bracts to be in a stacked structure) to serve as foodstuff can obviously improve the survival rate of the spodoptera frugiperda larvae, reduce the mutual killing risk, and realize the grouped breeding because the humidity in the insect box is too high and the larvae are infected with diseases and die. In addition, the eclosion rate of the examples 1 to 3 is significantly higher than that of the control group 1 (63.96%), which indicates that the sandy soil plays a good role in protecting the pupa at the later stage and improves the eclosion rate of the spodoptera frugiperda imagoes. The survival rate of larvae in the control group 2 is significantly lower than that in examples 1-3, which shows that the stacked structure of baby corn shoots can effectively reduce mutual killing of larvae and significantly improve the survival rate of spodoptera frugiperda larvae. Therefore, the multilayer stacked young corn cobs can be used for feeding spodoptera frugiperda larvae after a three-dimensional space structure is built, and the spodoptera frugiperda is fed in a large amount by using a method for collecting pupae by using sand, so that a large amount of uniformly-developed insect sources for the application of control technologies such as breeding natural enemy parasitic wasps and the like is provided for developing the biological ecology of the spodoptera frugiperda.
The above disclosure is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the scope of the present invention, therefore, the present invention is not limited by the appended claims.
Claims (10)
1. A grouinization feeding method for feeding spodoptera frugiperda larvae by using young corn shoots is characterized by comprising the following steps:
(1) preparing a Spodoptera frugiperda larva breeding box:
(1-1) paving sand at the bottom of the box;
(1-2) stacking the corn shoot bracts, the corn shoot cores and the corn stigma on the upper layer of the sand to form a multilayer three-dimensional space structure;
(1-3) placing corn stigma and young baby corn cores on the uppermost layer, and using the young baby corn stalks for feeding Spodoptera frugiperda of the low age;
(2) the larva breeding process:
(2-1) placing corn seedlings or kraft paper with spodoptera frugiperda egg masses on the corn silk and young baby corn cores on the surface layer;
(2-2) periodically replacing fresh baby corn after the larvae grow to 4 th instar, cleaning insect dung, simultaneously keeping a stacked structure among baby corn bracts, baby corn cores and corn stigma, avoiding mutual killing behavior caused by mutual contact of the larvae after 4 th instar, and enabling the aged pupated larvae to enter sand to pupate;
(3) collecting pupae, raising imagoes and storing eggs:
(3-1) after the aged larvae pupate in sand, screening out the aged larvae from the sand by using a sample separation screen, putting the aged larvae into a bottle, and standing at room temperature until pupae feather;
(3-2) putting the eclosion imagoes into an insect raising cage, and placing corn seedlings or kraft paper with edges torn into strips for the imagoes to lay eggs;
(3-3) replacing cotton balls and collecting egg blocks every day, wherein the relative humidity in a room where the insect cage is located is not lower than 70%;
(3-4) putting the collected spodoptera frugiperda egg blocks into a refrigerator with the temperature of 5-10 ℃ for storage for 1-5 days, and taking out the spodoptera frugiperda egg blocks and putting the spodoptera frugiperda egg blocks into a feeding box when the spodoptera frugiperda egg blocks are used.
2. The method for grouinized rearing of spodoptera frugiperda larvae with young corn shoots as claimed in claim 1, wherein the method comprises the steps of: in the step (1-1), a ventilation net opening is further formed in the box cover, sand in the box is paved to be 2-4 cm thick, and the sand is subjected to high-temperature dry heat sterilization.
3. The method for grouinized rearing of spodoptera frugiperda larvae with young corn shoots as claimed in claim 1, wherein the method comprises the steps of: in the step (2-1), a paper sheet for preventing larvae from escaping is placed under the box cover.
4. The method for grouinized rearing of spodoptera frugiperda larvae with young corn shoots as claimed in claim 1, wherein the method comprises the steps of: in the step (2), after the dispersed larvae take corn shoot cores and bracts to grow to 4 years old, when the population density is more than 5.5 heads/L, the larvae should be separated from the rearing boxes in time.
5. The method for grouinized rearing of spodoptera frugiperda larvae with young corn shoots as claimed in claim 1, wherein the method comprises the steps of: in the step (2-2), fresh young corn shoots are replaced for 2-5 days in the breeding process.
6. The method for grouinized rearing of spodoptera frugiperda larvae with young corn shoots as claimed in claim 1, wherein the method comprises the steps of: in the step (3-1), the sample separating sieve is 8-15 meshes, and the nylon net is 100-300 meshes.
7. The method for grouinized rearing of spodoptera frugiperda larvae with young corn shoots as claimed in claim 1, wherein the method comprises the steps of: and (3-2) putting the eclosion imagoes into an insect cage of a nylon mesh cloth of 100-200 meshes, placing corn seedlings or kraft paper with the edges torn into strips for the imagoes to lay eggs, and hanging cotton balls dipped with 10% honey water for the imagoes to supplement nutrition.
8. The method for grouinized rearing of spodoptera frugiperda larvae with young corn shoots as claimed in claim 1, wherein the method comprises the steps of: and (3-4) storing the collected spodoptera frugiperda egg blocks in a refrigerator at 5 ℃ for 1-5 days.
9. The method for grouinized rearing of spodoptera frugiperda larvae with young corn shoots as claimed in claim 1, wherein the method comprises the steps of: the young corn shoots are young corn shoots which are picked early for harvesting big and full corns, or the young corn shoots are fruits which are specially produced and cannot be picked all first until corns grow.
10. The method for grouinized rearing of spodoptera frugiperda larvae with young corn shoots as claimed in claim 1, wherein the method comprises the steps of: the indoor temperature of the feeding box and the insect cage is 20-30 ℃, and the photoperiod L: D is 12-16 h: 12-8 h.
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