TW564158B - Techniques for mass rearing of insects - Google Patents

Abstract

The present invention relates to a method for mass rearing of insects required in biologically preventing pests, particularly a method for mass rearing of insects which are natural enemies of and feeding on Aenaria lewisi. The method is consisted of a step of growing insect eggs and worms, a step of collecting adult worms, and a step of spawning and collecting eggs. Through the method, mass rearing can be carried out indoors with stable temperature and lighting. Using the biological natures of the insects on lighting, preying for food, and falling, a suitable environmental medium is provided for the insects in order to carry out a high density breeding and obtain eggs. The results indicate that a sufficient quantity of insects can be reared to perform biological prevention of pests while reducing the problems of pests such as thrips in the farm. Meanwhile, the problems of residual herbicide and the herbicide resistance of pests derived from an overuse of herbicide can be prevented.

Description

564158 _ Case No. 90112359_ Years and months Amendment _ V. Description of the invention (1) [Background of invention] A large number of natural enemy insects are released in the field to suppress the pest density, which is a commonly used biological control method for plant protection in modern agriculture. Pest control in this way can reduce farmers' losses, avoid the use of highly toxic pesticides, avoid environmental pollution, and help maintain a sustainable ecological environment. However, for such biological control, there must be a large number of natural enemy insects for use, and the production cost must be low to meet the economic benefits of agricultural cultivation. For example, thrips is a very serious pest that harms crops. The larvae and adult's sucking and spawning cause direct damage to plants, and they spread vector plant diseases, causing great economic losses. The use of general chemical agents to remove insects does not cost a lot, and has ineffective effects and resistance problems. It is difficult to control and biological control is an important control method. Among the adopted natural enemies, insect predators are the largest and most valued. There are many predatory species in this family Hemiptera, A nth occ eridae, Orius and Miridae, C ampy 1 omma, etc. Small insects such as horses, whiteflies, aphids, leaf shouts, and eggs of moths and beetles. There are many species with strong predation ability, which can be used in agriculture for biological control. Existing technologies have a predatory nature when collectively feeding this natural enemy insect, which is prone to the phenomenon of insects eating each other, which makes it difficult to reproduce in large numbers. As an example, thrips are used for field biological control. It takes about 10,000 predatory stink bugs per sub-land and cast more than four times to achieve the biological control effect. The estimated cost is based on the current US natural enemy production company such as ARBIC0. It costs only NT $ 200,000 for an adult insect at US $ 76.85, and the cost is very high.

Page 5 564158 _ Case No. 90112359 _ Revised Year of the Month _ V. Description of the Invention (2) Jiabaofu, Fenipni, etc. are likely to cause environmental pollution and cause poison to non-control target organisms. Long-term application and resistance The problem of pesticides and pesticide residues in agricultural products will also occur; therefore, it is the object of the present invention to seek a pest control method that has economic benefits and meets environmental protection requirements. [Summary of the Invention] The present invention relates to a method for breeding insects, which is used to propagate natural enemy insects required for the biological control of pests in modern agricultural plant protection, and particularly relates to a method for breeding predatory stink bugs as natural enemy insects. Flower stink bugs and blind stink bugs in predatory stink bugs are omnivorous predatory insects, and can be reared with small insects, such as aphids, thrips, whitefly, leaf pupae, moth eggs, and even pollen from plants such as pine and melon It can be used as its food. Adults lay their eggs on young plant parts, such as sprouts, young leaves, buds and other materials, can be used as their spawning medium. The life cycle and spawning can be completed indoors, but the development and reproduction rate is generally low, and the production cost is high, which is difficult to meet the economic requirements of biological control. Predatory stink bugs in Taiwan's melon gardens and eggplant gardens are common. Although they are small and have large predation, they inhabit a variety of plants and prey on small insects such as thrips, aphids, whitefly, and spider mites. Among them, they are best at predating thrips. For example, during the whole period of nymphs and adult worms in the southern small black stink bug, about 2 3 ~ 3 0 0 thrips, or 5 0-6 0 0 leaves are predatory, which is excellent in biological control effect. Natural enemy choice. This species of stink bug is native to Taiwan, mainland China, southern Japan and other places, and is very suitable as a species for Taiwan ’s climate environment for predatory thrips biological control. However, the predatory nature of this natural enemy insect makes it easy for insects to eat each other when they are collectively raised, and it is difficult to reproduce in large numbers, resulting in high breeding costs.

Page 6 564158 _Case No.% 112359_ Year Month Amendment _ V. Description of the invention (3) Excessive use makes it expensive to use. There are not enough incentives for farmers to adopt it, and the economic benefits are insufficient, which is inconsistent with biological control requirements. After the inventor's experimental research, a method of breeding insects in large numbers, especially the method of predatory stink bug reproduction; mainly includes three steps: the development of eggs and nymphs, the collection of adults, the laying of adults, and the collection of eggs. To increase the survival density of the worm in a specific space, and use fresh green pea seedlings and red lines to treat the never-hatched mealworm moth eggs, which can breed large numbers of predatory stink bugs in a room with stable temperature and light. The development of nymphs is a method of making many discontinuous spaces for nymphs to hide by using shredded paper strips and foamed plastic mesh sheets in the gauze bag to avoid the self-harm behavior caused by predation and achieve high-density breeding purposes; Feed on the eggs of the moth moth and the green pea seedlings, and then use the biological characteristics of light and falling to collect the insects externally; then place the adults into the plastic bag with the green pea seedlings as the oviposition medium to obtain the eggs. The nymphs hatched from the eggs can be used for biological control. The whole process of the insect raising method is simple and easy to operate, and the insect body develops rapidly. This insect raising technology improves the survival density of the insect in a specific space, the self-injury rate of the insect body is low, the reproduction and survival rate is high, the adult life is long, and the egg production is increased. It is in line with the economic benefits of feeding the worm in large quantities, suitable for biological control and reducing pest problems such as thrips in the field; generally easy to obtain materials and low cost, including reused fruit packaging nets and paper, and all worm containers can be recycled Re-use is in line with environmental protection principles. In the process, biological characteristics are often used to reduce the labor required for operation, so it can meet economic requirements. If the cost of production is analyzed, only 1,000 seedlings are needed for the production of 1,000 spring elephant eggs. The consumables of the white spot moth eggs, plus the electricity cost of feeding equipment and indoor reproduction control light and temperature, and

Page 7 564158 _Case No. 90112359_ Revised Year of the Month _ V. Description of the invention (4) The estimated labor cost is only about 50 yuan, which is far lower than the cost of biological control in the past, which is about the use of chemical killing. The cost of insecticides is completely free of pollution and pesticide residue problems, so it has a very high industrial use value, which is a newly developed technology for this type of natural enemy insect breeding. The basic conditions of the insects, such as the feed, the oviposition medium, the appropriate temperature for the insects, and the time of light, have been tested by the inventors and are the most suitable conditions for the development and reproduction of the insects. Adjust the survival density of the worm in a specific space, starting from 600-9 0 stink bug eggs per worm-raising net bag, the emergence rate of adult worms can reach more than 80%, and the self-harm rate of the worm body is Shredded paper and foamed plastic meshes make discontinuous space for hiding, which is reduced to meet the economic benefits of large numbers of worms. In addition, try to use materials that are generally easy to obtain, including the use of free fruit packaging plastic mesh and used paper to save costs. All insect culture containers, such as net bags and plastic bags, can be recycled, cleaned and reused, which is in line with environmental protection principles. It is an economical method of raising insects by collecting mature adult insects from the insect breeding bag using adult biological characteristics, saving operation labor, and being suitable for mass production and mass reproduction. The following examples are used to further illustrate the present invention, but these embodiments can only be used as examples rather than limiting the method adopted by the present invention, nor should they limit the content of the present invention. [Explanation of the preferred embodiment] As shown in the first figure, the method for raising the insects mainly includes three steps: the development of eggs and nymphs, the collection of adults, the laying of adults, and the collection of eggs. Reproduction, the development of nymphs uses a yarn

Page 8 564158 Case No. 90112359 Amendment V. Description of the Invention (6) Transparent plastic bag (2 1) with straight vent (2 1 1) built-in green pea seedlings, and eggs containing eggworm moth eggs, interface (2 2) In the insect net bag (Π) as an adult collecting bag, the plastic bag side faces the light source (2 3), and the insect net bag is covered with a black cloth (2 4) to create different light and dark environments, using predatory stink bugs For the biological characteristics of insect phototaxis, the adult (25) will automatically move into the plastic bag, and an adult collecting method can be achieved without extra manpower. Adults are collected every day for 3 consecutive days. Shake the sieve to collect the remaining 4 to 5th (that is, the 15th to 16th grown adults) in the net bag. For example, 2 0 pairs of white hair bean seedlings (1 powdery pupae bag. The sieve is separated and the moth eggs are increased. The number of seedlings can be considered. The number of enemy insects can be multiplied by three: the fourth figure of the adult, with a large foam plastic net 5) 30g,-moth eggs, insects (25) that is bean seedlings and Chengjia containing India. .. Collected about the breeding methods of the breeding party and the larvae of the larvae, and collected the eggs and collected the eggs. The collected predatory stinkbug adults were collected from 4 0 (about transparent with micro vents (32)). Plastic bag (31), built-in sheet 3 layers (1 3), each two layers sandwich the ovipositing medium green skin two layers a total of 60 g, and the infrared treatment will never be ridged 0.3g eggs, two layers A total of 0.6g, as eggs, will lay eggs (17) on green bean sprouts (15), and then use networms, bean sprouts are replaced every 2-3 days and egg collection is completed, 2g, and processed by infrared Seven times of never hatching powdery maggots, use a dissecting microscope to inspect the adult when every 100 eggs of beans are reduced, and use the maximum economic worm egg output to develop the artificial control method for large-scale reproduction of biological control methods, especially It is a method for predatory stink bugs as natural enemy insects, for biological control of thrips crops in the field, using natural enemies to play a role in controlling pests, and improving control effectiveness

Page 10 564158 _Case No. 90112359_ Year Month Amendment _ V. Description of the invention (7) Fruits, reduce field medication. However, the above description is only one possible embodiment of the present invention. For example, any equivalent structural change made by using the description of the present invention or the scope of patent application should be included in the patent scope of the present invention.

Page 11 564158 Case No. 90112359 Revised map of the month and day of the worm and the eggs into the middle of China and France Fandan Lidan Lidan Lidan Lidan greatly large insects insects insects insects Kunkun Shikun Kun elephants like real elephants Chun Chun and Chun Chun Chun Chun Schematic sexual applicator food feeding food feeding And catch and 1 kind of step species, seed set, set one, one set, one set, one set _ said :: hair: step: step diagram of a single chart diagram ^ picture one two insects three episodes four prints Soudi [Illustration of the drawing number] Insect net bag (11) Beam mouth (12) White foamed plastic mesh (1 3) Shredded paper (1 4) Green bean sprouts (1 5) White spot moth egg eggs (1 6) Predatory stinkbug eggs (1 7) Predatory stinkbug nymphs (1 8) Light source (1 9) Transparent plastic bag (2 1) Ventilation holes (2 11) Mesh bag and plastic bag interface (2 2) Light source (2 3) black cloth (2 4) adult (2 5)

Page 12 564158 Case No. 90112359_Year Month and Day Amendment Brief Description of Drawings Transparent Plastic Bag (3 1) Micro Air Vent (3 2) Page 13

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Claims (1)

564158 _Case No. 90112359_ Years and months of amendments_ 6. Application for patent scope 1. An insect breeding method for mass reproduction of insects, which includes I. Eggs and nymphs development steps: (1). Will contain predatory stinkbug eggs Green bean sprouts are placed in white light-transmitting insect net bags for breeding; (2). White foam plastic nets are placed on the upper and lower layers of the insect net bags, and shredded paper is placed in the middle to create a hiding space to avoid predatory self-harm. Behavior, improve survival rate, and maintain a certain humidity in the insect culture bag to keep the bean sprouts from spoiling; (3). Put the green bean sprouts between the shredded paper and the mesh and the eggs of the white spotted moth that will never hatch after infrared treatment. Feed, and then put it in the mouth; (4). Every 2-3 days, add seedlings in the upper and lower layers of the bag and incubate the eggs of the white moth moth that will never hatch after infrared treatment to maintain the development of nymphs; Π. Collecting steps for adults : (1). Transparent green plastic bags with vent holes and built-in green bean sprouts, and eggs of powdery moth moth that will never hatch after infrared treatment are connected to insect net bags as adult collection bags; (2). Face the adult collecting bag towards the light source, The insect net bag is covered with a black cloth to create different light and dark environments; (3). The nymphs will become adults from the 12th day, and will automatically move to the adult collection bag with phototaxis biological characteristics; (4). Collect adult worms from the adult worm collection bag for 3 consecutive days to 4-5th (that is, the 1st 5th to 16th of the growth), and shake the sieve to collect the remaining Page 14