KR20190027226A - Artificial egg bag for rearing Oorencyrtus nezarae - Google Patents

Abstract

The present invention relates to an artificial egg bag for artificially breeding Ooencyrtus nezarae. In particular, the artificial egg bag of the present invention is characterized by the following: (i) the artificial egg bag has a sealed bag form, wherein two sheets of polypropylene films are attached to each other around edges thereof; (ii) artificial eggs, each having a semi-spherical form sized to a diameter of 2.0 to 2.5 mm and a height of 2.0 to 2.5 mm, are formed on an upper end of one sheet of the polypropylene films; (iii) artificial cultures containing haemolymph of larvae of Antheraea pernyi are distributed in an amount of 3 to 5 μl in each artificial egg; and (iii) surfaces of the artificial eggs are coated with abdominal extracts of soldier bugs. The artificial egg bag of the present invention may be used to artificially breed Ooencyrtus nezarae in large batches, thus sparing efforts put into naturally breeding Ooencyrtus nezarae using the eggs of soldier bugs as actual natural host eggs. Also, using the artificial egg bags in artificially breeding Ooencyrtus nezarae may serve to practically control Riptortus pedestris, which is a key pest of legumes and fruit trees.

Description

{Artificial egg bag for rearing Oorencyrtus nezarae}

The present invention relates to artificial pouches for artificial breeding of O. vulnificus . More specifically, the present invention relates to artificial pouches for raising artificial poultry without using natural poultry eggs as natural host eggs, Artificial pouch.

The dominant species of Riptortus pedestris , which causes the greatest damage in domestic soybean crops and edible meats , is low mobility and low efficacy using medicines (Lim, UT 2013. Korean J. Appl. Entomol. 52 (4 ): 437-448).

Ooencyrtus nezarae is a pest of beans, crops and fruits (persimmon, apple, pear, etc.): Riptortus pedestris , Anacanthocoris concoloratus , A. sordidus , ( Eg , Chauliops fallax , Cletus punctiger , C. rusticus , Dolycoris baccarum , Eysarcoris guttiger , Homoeocerus unipunctatus , H. marginiventris, ), Leptocorisa chinensis , Megacopta punctatissimum , Nezara antennata , Nezara viridula , Piezodorus hybneri , P. guildinii , and Plautia stali ) Egg parasitoid (Lim, UT 2013. Korean J. Appl. Entomol. 52 (4): 437-448).

Ooencyrtus nezarae has been studied in Japan and Korea as an important nematode controlling the population density of the backbone ants, and emphasis has been placed on the control of the backbone antlers (Takasu, K Paol, C.-H., et al., 2007a., J. Appl. Entomol. 46 (2): 275-7, 280, Paik, C.-H., et al., 2007b, Korean J. Appl. Entomol. 46 (2): 281-286; Alim, Md. Entomology 13: 139-143).

Hop test of species belonging to the genus al wasps the Ooencyrtus belonging to the jombeol vitro (in vitro) from the result of raising the wasps in Ooencyrtus pityocampae of Thaumetopoea pityocampa feat nabangryu one kinds of a paper, Italy on artificial breeding using artificial Al and artificial media (5). In this study, it was found that the number of adult adults was significantly higher than that of the control group (Masutti et al., 1993, Entomophaga. 38 (3): 327), and the domestication of Lymantria dispar , Ooencyrtus kuvanae (Lee, H.-P., et al., 1994, Korean J. Entomol. 24 (4): 311-316).

On the other hand, in Japan, eggs of egg-laying eggs that were scattered on the eggs of the back leg ants were taken out and grown in artificial medium in the test tube. As a result, milk (cow milk) 25%, chicken egg yolk 25% (1), and the final survival rate was 72.5% (Takasu, K. et al., 1992, Appl. Entomol. Zool. : 171-173).

However, there is no report yet on the artificially propagating technology of artificially propagating eggs.

Accordingly, the present invention can reduce the effort of raising parasitic nematodes of the back leg ants, using a natural host, and can be applied to practical control purposes by artificially raising nematodes in large quantities The inventors of the present invention have completed the present invention by developing artificial eggs capable of artificially cultivating artificial larvae of the eggs in the absence of the artificial eggs of the back leg ants.

Accordingly, a technical problem to be solved by the present invention is to provide artificial eggs that can be artificially mass- cultured without using natural host eggs, which is a parasitic nematode of omnivores ( Oorencyrtus nezarae ).

Another object of the present invention is to provide a method for producing artificial eggs.

In order to solve the above technical problems, the present invention provides an artificial egg bag for artificial breeding of Oerencyrtus nezarae , comprising: (i) a double-layered polypropylene film having edges sealed together to form a bag- (ii) a semi-spherical artificial egg having a diameter of 2.0 to 2.5 mm and a height of 2.0 to 2.5 mm is formed on the top of a single-layer polypropylene film of the polypropylene film, and (iii) An artificial medium containing an artificial egg Antheraea pernyi pupa haemolymph is dispensed in an amount of 3 to 5 μl, (iv) the artificial egg is coated with the abdomen extract of a yellowing substance It provides an artificial egg bag for artificial breeding of the eggs.

According to another aspect of the present invention, there is provided a method of manufacturing an artificial egg bag for artificial breeding of an egg hopper, comprising the steps of:

(S1) forming hemispheric artificial eggs having a diameter of 2.0 to 2.5 mm and a height of 2.0 to 2.5 mm at the top of a single-layer polypropylene film;

(S2) dividing the artificial egg into an artificial medium containing an Antheraea pernyi papillary haemolymph in an amount of 3 to 5 μl;

(S3) folding the single-ply polypropylene film with the artificial eggs in half to engage the edges or overlapping the other single-ply polypropylene film to engage and seal the edges to form a bag-form ; And

(S4) a step of applying abdomen extract of yellowing material to the surface of the artificial egg.

According to one embodiment of the present invention, the nodule is a natural host of Ooencyrtus nezarae , such as Riptortus pedestris , Anacanthocoris concoloratus , A. sordidus , Chauliops fallax , Cletus punctiger , C. rusticus , Dolycoris baccarum , Eysarcoris guttiger , Homoeocerus unipunctatus , H. marginiventris , Leptocorisa chinensis , Megacopta punctatissimum , Nezara antennata , Nezara viridula , Piezodorus hybneri , P. guildinii , and brown wing nematode , Plautia stali ).

According to a preferred embodiment of the present invention, the thickness of the polypropylene film constituting the artificial nail bag for artificial breeding of the eggs is 0.3 to 0.5 mm, preferably 0.4 mm, The artificial egg can be formed without tearing the film when the egg is formed, the hemispherical shape of the artificial egg can be maintained as it is, and any thickness can be used as long as the eggshell can be scattered.

The artificial egg may be made to have a size similar to that of the nymph or a natural host of the nymphs or slightly larger than those of the nymphs, and may be manufactured to a size such that the artificial medium can be dispensed in an amount of 3 to 5 μl. For example, it is possible to manufacture the bobbin having a diameter of 2.0 to 2.5 mm and a height of 2.0 to 2.5 mm, preferably 2.2 mm in diameter and 2.2 mm in height (see FIG. 7).

According to a preferred embodiment of the present invention, hemispherical artificial eggs having the above-mentioned size can be made by using an iron rod with a rounded end on a polypropylene film.

According to a preferred embodiment of the present invention, the artificial eggs formed on the polypropylene film of the artificial egg bag for artificial feeding of the egg hopper can be formed at intervals enough to scatter eggs without interfering with each other, For example, 4 to 6 mm, preferably 5 mm, on the polypropylene film.

The bag-shaped artificial egg bag of the present invention is obtained by folding a single-layered polypropylene film having the artificial egg in half in order to engage the edges or overlapping the other single-layered polypropylene film, bag-form. (C) 5 cm, a length (d) 2 cm, and a height (e) of 0.5 cm in the width (c) of 4 to 6 cm, the length (d) of 1 to 3 cm and the height (See FIG. 7), but there is no particular limitation thereto. At this time, artificial eggs of 10 to 14, preferably 12 artificial eggs may be formed on the polypropylene of one bag-shaped artificial nest.

In the present invention, microbial contamination such as fungi from the outside can be prevented by sealing the artificial egg bag in a bag-shaped form, and the artificial medium dispensed to the artificial egg can be prevented from being dried by the outside air.

According to a preferred embodiment of the present invention, an artificial medium for artificial breeding of eggs is an agar medium containing Antheraea pernyi pupa extract as an effective ingredient as haemolymph, specifically Antheraea pernyi It is characterized by containing pupae haemolymph, chicken yolk, infant formula, Neisenheimer salt solution, penicillin and streptomycin.

According to a preferred embodiment of the present invention, an artificial medium for artificial rearing of eggs is distributed in an amount of 3 to 5 μl, preferably 4 μl, per artificial egg, This is the amount required to scatter up to 7 eggs. When eggs are fed at an amount of less than 3 μl, it is not sufficient for eggs to be scattered. When the eggs are fed at an amount exceeding 5 μl, not.

According to a preferred embodiment of the present invention, it is possible to scatter eggshell eggs by applying an abdominal extract of a yolk to a artificial egg without using eggs of an egg yolk, which is a natural host of the eggshell, and the abdomen extract of the yellow- Can be removed by immersing in the organic solvent for 60 to 80 hours. The organic solvent may be methanol, ethanol, propanol or butanol, preferably ethanol.

According to one specific embodiment of the present invention, artificial spawning of an egg-hopper beetle can be induced by inoculating an eggshell beetle larvae artificially foddered to artificially raise an egg beetle.

According to one specific embodiment of the present invention, in a bag-shaped artificial egg bag for artificial feeding of egg hopper bees, 15 to 25 eggs of 4 to 6 days old alopecia areophilized per two artificial egg bags, preferably And 20 mice are inoculated.

According to one specific embodiment of the present invention, eggshell eggs inoculated into artificial egg bags can be developed into adult eggs via artificial eggs by scattering eggs and larvae and pupa stages. Specifically, the average number of eggshell eggs scattered in artificial egg bags was 185 per artificial egg bags, and the average recovery rate of adult eggs was 46.1%, and development period ranged from eggs to larvae It took 16-20 days from work, egg to pupa, and it took 7 days on average. In addition, sex ratio of male adults harvested from artificial eggs was 1: 1.64 in male and female was almost twice as many as male.

As a result of the above artificial raising using the artificial pouch for artificial breeding of the beetle according to the present invention, the development was delayed about 3-5 days compared to the natural breeding, and the emergence rate was 46.1% But it is meaningful to be able to artificially nurture the parasitic nematode, which is a parasitic nematode, without using the nematode eggs as natural host eggs. Therefore, it is expected that artificial rearing of the eggs of artificial eggs using these artificial eggs will be enough to be used as biological control agents in agricultural fields in the future.

Meanwhile, artificially scattered eggshell eggs in artificial egg bags according to the present invention can be alienated into adult eggs through larval and pupa stages in artificial egg bags. When artificial eggs are grown into adults, they can be used to open or seal the sealed part of the artificial egg bag, spraying artificial reared artisanal beetles with soybeans, crops, It can be used as a biological control source of phosphorus, which is a pest of persimmon.

As described above, by using the artificial egg bag according to the present invention, it is possible to reduce a lot of efforts for natural breeding by using artificial largemouth eggs as natural host eggs by artificially cultivating oerencyrtus nezarae . In addition, it is expected that artificial eggs will be used as a practical control agent for artificially infested birds, which are the most damaging to beans, crops, and oranges.

Fig. 1 is a photograph showing the breeding of a sawtooth leg ( Riptortus pedestris ) in a soybean plant.
Fig. 2 is a picture showing the breeding of an oarencyrtus nezarae .
Fig. 3 is a photograph showing the process of using a natural host egg, Riptortus pedestris egg, for natural spawning of Oorencyrtus nezarae .
FIG. 4 is a graph showing the scattering ability of eggshell eggs using natural host eggs. FIG.
FIG. 5 is a graph showing the cumulative number of eggs scattered in the natural host eggs according to the scattering time. FIG.
FIG. 6 is a schematic view showing a process for producing artificial egg bags for artificial breeding of Oerencyrtus nezarae .
Fig. 7 is a schematic illustration of artificial egg bags prepared for artificial breeding of Oorencyrtus nezarae .
Fig. 8 is a photograph showing the process of pupaing in the spawning of an artificial egg ( Oorencyrtus nezarae ) using artificial eggs.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Example 1 Experimental Insect Breeding

The top leg ants, which are the hosts of the eggshells, are used as food plants in the forest (75 ± 75x75cm) at the feeding room (26 ± 1 ℃, 35-40% RH, L16: D8) The feed was fed while being fed as a feed source, and the host eggs were obtained. Fig. 1 is a photograph showing the appearance of a sawtooth leg ( Riptortus < RTI ID = 0.0 > pedestris ).

On the other hand, eggs were collected from the eggs of the back leg ants in the cultivation area around Sunchon University in Jeollanam - do, Jeollanam - do. (25 ± 1 ° C, 45% RH, L16) in a plastic square cage (7 × 7 × 10 cm) treated with a manganese sachet on top of the top cover and inoculated with eggshells, : D8), the densities required for the experiment were maintained throughout the incubation. Fig. 2 is a picture showing the breeding of an oarencyrtus nezarae .

Reference Example 1: Scattering measurement using a natural host egg of a beetle

In order to investigate the egg laying ability of natural host eggs, Riptortus pedestris eggs (natural host eggs) were collected and stored frozen (4 ℃) for up to 2 days. (25 ± 1 ° C, 45% RH, L 16: D 8) were fed with 10 female oocytes ( Oorencyrtus nezarae ) within a given hour.

Fig. 3 is a photograph showing the process of using a natural host egg, Riptortus pedestris , for natural spawning of Oorencyrtus nezarae . Here, A is a picture of a natural host egg (n = 100) of a sawtooth antler for use in natural spawning, and B is a photograph of an egg hopper using natural host eggs for spawning.

Natural host egg female cock gun Sex ratio
(♀: ♂) n = 10 2.90 ± 1.45 1.60 + 1.07 4.50 ± 2.07
(0 - 7) 1.81: 1

As shown in Table 1, the number of laying eggs per natural host egg of the back leg ants was about 4.50 ± 2.07, up to 7 eggs per egg, 1: 1.81, and the female was twice as much alienated.

In order to investigate the egg laying ability of natural host eggs, we collected the eggs from the back leg ants (natural host eggs) and frozen for up to 30 days (4 ℃) 10 eggs were given to the female, and 100 eggs were spawning every day.

FIG. 4 is a graph showing the scattering ability of eggshell eggs using natural host eggs, and FIG. 5 is a graph showing the cumulative eggshell count of eggshell eggs obtained from natural host eggs according to scattering time. As shown here, spawning occurred until the 18th day after parasitic administration, and 65% of the total number of eggs was scattered within one week, and the eggs were scattered the most during 5-6 days. A total of 254 adults (85 males and 169 females) were alive in the breeding, and the sex ratio was 1: 1.99. Cumulative number of eggs laid per female was 25.4 on average.

<Example 1> Breeding of an egg-hopper beetle using artificial eggs

(1) Artificial medium composition

The composition of the artificial medium used in this experiment are described in: gypsy seen hop jombeol (Ooencyrtus kuvanae) described in (Lee, H.-P., et al, 1994, Korean J. Entomol 24 (4).. 311-316) Antheraea pernyi pupa was used as a haemolymph according to the composition shown in Table 2 based on the contents of the medium used in artificial breeding.

As a hemolymph, the pupae were treated with water at 60 ℃ for 4 minutes and used. The compound nutrients required for the larval development were used for the infant formula.

ingredient weight (%) Antheraea pernyi (haemolymph) 40 Egg yolk (Chicken yolk) 20 Infant formula 30 Neisenheimer salt solution 10 Penicillin 400 units / ml Streptomycin 400 units / ml

(2) Manufacture of artificial eggs

FIG. 6 is a schematic view showing a process for producing artificial egg bags for artificial breeding of Oerencyrtus nezarae . (10) is an artificial egg, (11) is an artificial medium, (101) is another single-layer polypropylene film, (12) is a sealed one (102) is an artificial hand pocket.

Fig. 7 is a schematic illustration of artificial egg bags prepared for artificial breeding of Oorencyrtus nezarae . Here, a represents the hemisphere height of the artificial egg, and b represents the diameter of the artificial egg.

6 and 7, hemispherical artificial eggs (2.2 mm × H 2.2 mm) 10 are formed on a polypropylene film 100 having a thickness of 0.4 mm by using an iron rod having a rounded end, And 12 artificial eggs (10) were formed on one polypropylene film (100) at intervals of 5 mm. One artificial alginate artificial medium (11) was dispensed in 4 mu l each. In order to supply the required oxygen for the development of the eggs and to prevent contamination of microorganisms such as fungi from the outside, the bag-type is sealed (12) with a size of 5cm x 2cm x 0.5cm in height using a vinyl adhesive . All processes for making artificial pouches were carried out in a clean bench to prevent contamination of germs.

(3) Extraction and application of scattering inducer

In order to induce the egg-laying larvae to scatter in the artificial eggs, 100 stomachs (about 3.7 g) of the dorsal flank of the dorsal flank were removed and placed in 2,000 쨉 l of ethanol as an organic solvent. After 72 hours of immersion, The artificial eggs formed on the propylene film were applied to each artificial egg surface with a capillary pipette at a volume equivalent to one egg equivalent.

(4) Spawning of eggshells using artificial eggs

Four days, five days, and six days old twenty eggshells of each artificial egg bag produced in the bag-form artificial egg bags prepared above were exposed for 1 day (24 hours) The egg production rate was measured.

Females Back-form
(12 artificial eggs) Scattered eggs
/ Back-form Scattered eggs
/ Artificial eggs Fourth adult (20) Primary 92 7.67 ± 6.85 Secondary 106 8.33 + - 5.83 5th adult (20) Primary 108 9.00 5.12 Secondary 46 3.83 + - 4.43 6th adult (20) Primary 117 9.75 + - 6.55 Secondary 86 7.17 ± 6.89 Average 6 92.5 7.71 6.12

As shown in Table 3, the number of eggs scattered in the artificial eggs was 92.5 per bag-shaped artificial pouch and 7.71 in average per artificial egg.

The number of eggs scattered in one artificial egg was 1.7 times larger than the number of eggs scattered in natural host eggs compared with the natural egg scattering of Reference Example 1. The size of artificial eggs (2.2 mm × H 2.2 mm) It is thought to have been scattered a lot because it is larger than the egg size of natural host (about 1.4 mm).

Fig. 8 is a photograph showing the process of pupaing in the spawning of an artificial egg ( Oorencyrtus nezarae ) using artificial eggs. Here, A is a photograph of a spawning egg in an artificial egg, B is a photograph showing an egg stalk protruding from a polypropylene film of an egg shell and artificial egg shell, And D is a photograph showing the pupa stage.

As can be seen, the egg was a typical beetle egg with a noticeably protruding sack from the artificial egg (Fig. 8B). It remains attached to the back end of the egg shell during the life of the larva, and the produced sludge accumulates here. Upon reaching the dedicated phase, the meconium is discharged into several brown-orange pellets and the insect turns yellowish white (FIG. 8C). The molted pupa is initially yellowish, and later turns black (FIG. 8D).

(5) Development of egg-hopper beetle using artificial egg bag

Four days, five days, and six days after the emergence of two bag-shaped artificial pouches each having the artificial eggs prepared were exposed for 20 days each for 1 day (24 hours) After egg laying, the number of artificial egg per year, number of developed larval water, rate of solubilization, rate of adult emergence and sex ratio were investigated. The results are shown in Table 4 below.

Females An egg scattered in artificial eggs Yoo
(%) From eggs to larvae (days) Number of pupae (%) From eggs to puppies Duration (days) Adult Number
(%) Sex ratio
(♀: ♂) Fourth adult (20) 198 128
(64.6%) 9 - 13 104
(52.5%) 16 - 20 96
(48.5%) 63: 33 (1.91: 1) 5th day adult (20) 154 110
(71.4%) 9 - 13 79
(51.3%) 16 - 20 58
(37.7%) 35: 23 (1.52: 1) 6th day adult (20) 203 130
(64.0%) 9 - 13 109
(53.7%) 16 - 20 102 (50.2%) 61: 41 (1.49: 1) Average 185 122.7 (66.3%) 9 - 13 97.3 (52.6%) 16 - 20 85.3 (46.1%) 1.64: 1

As shown in Table 4, the average number of eggs scattered in artificial eggs was 185 per two bag-shaped eggs, and the recovery rate was 46.1% as an adult by hatching. The development period was 9-13 days from egg to larva To pupa was 16-20 days, and the period of pupa was 7 days on average. The sex ratio of adults harvested from artificial eggs was 1: 1.64, which was almost similar to the sex ratio of 1: 1.81 in the natural host eggs of Reference Example 1.

In this connection, the developmental period of the eggs in the natural host (sawtooth ankle) was studied in the literature (Takasu, K. et al., 1992, Appl. Entomol. Zool. 27 (1): 171-173) The results of this study are as follows: It takes 13-15 days from eggs to fables in the egg period of 2 days, 4-5 days of the larval stage, 7-8 days of the pupa, and the emergence rate is 96.3% To falconry takes about 16-20 days, so the development of about 3-5 days is delayed, and the allegorization rate is 46.1%, which is about half the level of natural host.

<Example 2> Measurement of scattering effect according to hemolymph type of artificial medium

In order to measure the scattering effect according to the type of haemolymph of the artificial medium, the pupa of Antheraea pernyi , the chrysalis of Caligula japonica , the brown dwarf larva, After culturing the medium, the number of eggs and their growth pattern were compared.

The chrysanthemum chinensis was collected in Gangwon province for two years in 2015 and 2016. During the period, chrysanthemums were collected and refrigerated, and hemolymphs were extracted and used. In the Laos market, Extracts were taken and used. Tenebrio molitor species larvae were easily available because they were kept as edible insects. Sowing larvae were used in laboratory.

After extracting hemolymph from each insect, the artificial pouch was prepared as the main component of the artificial medium in the same manner as in Example 1, and then 10 eggs were given for 5 days to lay eggs for breeding Respectively. The results are shown in Table 5 below.

Haemolymph Scattered eggs Yoo
(%) From eggs to larvae (days) Number of pupae (%) From egg to pupa
Duration (days) Adult Number
(%) Chrysalis of the chestnut Sanewan moth ( Caligula japonica ) 75 57 (76.0%) 21.40 ± 1.20 5 (6.7%) 6.8 ± 0.74 5
(6.7%) Antheraea moth ( Antheraea pernyi ) Pupa 113 65 (57.5%) 10.44 + - 3.37 18 (15.9) 5.5 ± 0.95 18 (15.9%) Brown duck ( Tenebrio molitor ) 3 0 ※ Not hatched Ripper ( Riptortus pedestris ) Species larva 0 - ※ No scattering observed

As a result, as shown in Table 5, no spawning induction reaction was observed in the artificial egg bag using hemolymph extracted from the adult larva of the back leg antler, and in the artificial egg bag using hemolymph extracted from the brown goat egg larva Spawning was done on some eggs, but development did not progress any more. In the early experiments, the artificial pouches from the chimpanzee Sambroot moth and Sambrooki pupa were recovered from the eggs. However, in the case of the chestnut moth, the egg - larvae period was considerably long and the solubilization rate was considerably high fell. In the case of the moth, the larval period was shortened to some extent and the solubilization rate was improved.

&Lt; Example 3 > Comparison of vitality of eggshells raised in natural host eggs and artificial eggs

The length (㎛) of hind leg calf nodes was measured and compared with 20 birds each using a dissecting microscope (100X) in order to compare the vitality of the eggshells raised in natural host eggs and artificial eggs. In addition, it was confirmed that adult eggs that were alienated from artificial eggs were inoculated again into natural host eggs and artificial eggs to spawn vigorous eggs. The results are shown in Table 6 below.

Number female Male Artificial egg 20 278.75 ± 38.21a 262.15 + - 31.28a Natural host egg 20 317.50 ± 42.57a 255.50 + - 34.79a

As shown in Table 6, the length of females alienated from the artificial eggs was slightly shorter than that of the algae of the natural host ( R. pedestris ), but no significant difference was observed , And there was almost no difference in the eggs of artificial or natural host in males. As a result of inoculation of females grown in artificial eggs again on natural host eggs and artificial eggs, live eggs were scattered and new adults could be obtained from them. Therefore, it is considered that the vitality of the adults developed in the artificial eggs is not different from the vitality of the adults developed in the natural host eggs.

As a result, this experiment has provided a basis for artificially raising alpine beetles, which are useful birds of parasitoids, as a useful source of nodules. Can be reduced. In addition, it is expected that artificial eggs will be used as a practical control agent for artisanal beetle, which is the biggest damage to beans, crops, and oranges.

100: one layer of polypropylene film
101: Another layer of polypropylene film
102: artificial egg pocket
10: Artificial eggs
11: Artificial badge
12: Sealing

Claims (8)

An artificial nail for artificial breeding of O. vulgaris , comprising: (i) a two-ply polypropylene film having edges sealed to form a sealed bag-form; (ii) A semi-spherical artificial egg having a diameter of 2.0 to 2.5 mm and a height of 2.0 to 2.5 mm is formed on the top of a single-layer polypropylene film of the film, (iii) an artificial egg of Antheraea pernyi (iv) an artificial medium containing an artificial medium containing haemolymph is dispensed in an amount of 3 to 5 μl, and (iv) an artificial egg is coated on the surface of the artificial egg with an abdomen extract of artemisia. An egg pocket. The method according to claim 1,
The above-mentioned barnyardgrass is selected from the group consisting of Riptortus pedestris , Anacanthocoris concoloratus , A. sordidus , Chauliops fallax , Cletus punctiger , C. rusticus , Dolycoris baccarum , Eysarcoris guttiger , Homoeocerus unipunctatus , H. marginiventris , Leptocorisa chinensis , Megacopta punctatissimum , Nezara antennata, ), southern pulsaek norinjae (Nezara viridula), horizontal lines norinjae (Piezodorus hybneri), pulsaek norinjae (P. guildinii) and brown wing norinjae (Plautia stali), the eggs hop jombeol, characterized in that one or more selected from the group consisting of artificial norinjaeryu Artificial pouch for breeding. The method according to claim 1,
Wherein the polypropylene film has a thickness of 0.3 to 0.5 mm. The method according to claim 1,
Characterized in that the artificial eggs are formed on the polypropylene film at intervals of 4 to 6 mm, in order to artificially cultivate the eggs. The method according to claim 1,
Wherein said artificial medium comprises antheraea pernyi pupa haemolymph, chicken yolk, infant formula, Neisenheimer salt solution, penicillin and Streptomycin. Artificial egg pouch for artificial breeding of characteristic Oorencyrtus nezarae . The method according to claim 1,
Characterized in that the abdomen extract of the yellow marrow is taken out of the abdomen of the yellow marrow females and is extracted by immersing in an organic solvent for 60 to 80 hours so as to artificially cultivate an egg hopper. The method according to claim 1,
Characterized in that the bag-form is 4 to 6 cm wide, 1 to 3 cm long and 0.2 to 1 cm high. A method for manufacturing an artificial egg bag for artificial breeding of an egg hopper according to any one of claims 1 to 7, comprising the steps of:
(S1) forming hemispheric artificial eggs having a diameter of 2.0 to 2.5 mm and a height of 2.0 to 2.5 mm at the top of one layer of the polypropylene film;
(S2) dividing the artificial egg into an artificial medium containing an Antheraea pernyi papillary haemolymph in an amount of 3 to 5 μl;
(S3) folding the single-ply polypropylene film with the artificial eggs in half to engage the edges or overlapping the other single-ply polypropylene film to engage and seal the edges to form a bag-form ; And
(S4) a step of applying abdomen extract of yellowing material to the surface of the artificial egg.

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