KR102086772B1 - A method for artificial rearing Oorencyrtus nezarae - Google Patents

Abstract

The present invention provides a method of manufacturing the artificial medium containing Al hop jombeol relates to a method for the artificial breeding (Oorencyrtus nezarae), (S1) sannue moth (Antheraea pernyi) pupa extract haemolymph (haemolymph); (S2) forming artificial eggs on one layer of polypropylene film; (S3) dispensing the artificial medium prepared in the step (S1) to the artificial egg formed in the step (S2); (S4) In step (S3), the artificial medium is divided into a single layer of polypropylene film formed with artificial eggs dispensed in half to engage the edges or to overlap the edges and overlap with the other one layer of polypropylene film bag ( manufacturing an artificial egg bag made of a form of a bag); (S5) applying the abdominal extract of the stink bug on the surface of the artificial egg of the step (S2); And (S6) according to the present invention comprising the step of inoculating and spawning female antler zombie hatching in the artificial eggs of the step (S5), a lot of effort that is consumed in the natural breeding using the stink bug eggs as actual natural host eggs Can be reduced. In addition, it is expected to be used as a practical control agent for the sawtooth ant loin norin, which causes the greatest damage in legumes and fruit trees by artificial breeding worms using these artificial egg bags.

Description

A method for artificial rearing Oorencyrtus nezarae}

The present invention relates to a method of artificial breeding of worms ( Oorencyrtus nezarae ), more specifically a method of mass artificial breeding using artificial eggs of parasitic eggs of stink bugs without using stink eggs as natural host eggs. It is about.

Riptortus pedestris , which causes the most damage in domestic legumes and orchards among stink bugs, has a high mobility and is less effective to control with drugs (Lim, UT 2013. Korean J. Appl. Entomol. 52 (4 ): 437-448).

The horned bee ( Ooencyrtus nezarae ) is a pest of legumes and fruit trees (sweet persimmons, apples, pears, etc.) and stink bugs: Riptortus pedestris , Anacanthocoris concoloratus , A. sordidus , and crab sting . ( Chauliops fallax ), Clevis punctiger , C. rusticus , Alkali vulgaris ( Dolycoris baccarum ), Eysarcoris guttiger , Homoeocerus unipunctatus , Mung bean halibutris ( ris. ), Leptocorisa chinensis , Sugarless alpine material ( Megacopta punctatissimum ), Grass-colored yellow leaf ( Nezara antennata ), Southern grass-colored yellow leaf ( Nezara viridula ), Piezodorus hybneri , P. guildinii , Brown-winged green leaf ( Plautia stali) (Eg, parasitoid) (Lim, UT 2013. Korean J. Appl. Entomol. 52 (4): 437-448).

Ooencyrtus nezarae has been studied in Japan and Korea as an important natural enemy of controlling the population density of Sawtooth ant-Hingerwood , and the use of this species has emphasized the control of Sawtooth- Herton Sterling (Takasu, K). et al., 1992, Appl. Entomol.Zoopl. 27 (1): 171-173; Paik, C.-H., et al., 2007a.Korean J. Appl.Entomol.46 (2): 275- 280; Paik, C.-H., et al., 2007b, Korean J. Appl. Entomol. 46 (2): 281-286; Alim, Md. A., et al., 2010, Journal of Asia-Pacific Entomology 13: 139-143).

Hop test of species belonging to the genus al wasps the Ooencyrtus belonging to the jombeol vitro (in vitro) from the result of raising the wasps in Ooencyrtus pityocampae of Thaumetopoea pityocampa feat nabangryu one kinds of a paper, Italy on artificial breeding using artificial Al and artificial media Five adults were obtained (Masutti et al., 1993, Entomophaga. 38 (3): 327), and domestically, Ooencyrtus kuvanae , a parasitic rod of Lymantria dispar , was the best. An adult allegory rate of 42.1% was obtained (Lee, H.-P., et al., 1994, Korean J. Entomol. 24 (4): 311-316).

On the other hand, in Japan, the eggs of egg-laying bees scattered on the eggs of Saw-legged hen, and raised by using artificial media in vitro, milk milk (25%), egg yolk (chicken egg yolk) 25% 1% larval survival rate and 82.5% larval survival rate were obtained in a medium containing 50% silkworm hemolymph (Takasu, K. et al., 1992, Appl. Entomol. Zool. 27 (1)). : 171-173).

However, there are no reports on the technique for artificially multiplying the swarm beetle.

Therefore, in the present invention, to reduce the amount of effort consumed in the breeding of parasitic eggworm beetle of the stink bug using a natural host, to develop a method that can be applied to the actual control purposes by artificial breeding eggworm beetle in large quantities The present invention was completed by developing a method for artificially raising large numbers of worms without using stink bug eggs.

Therefore, the technical problem to be solved in the present invention is to provide a method for artificially raising a large number of parasitic worms ( Oorencyrtus nezarae ) of stink bugs without using natural host eggs.

In order to solve the above technical problem, the present invention provides a method for artificial breeding worm beetle ( Oorencyrtus nezarae ) characterized in that it comprises the following steps:

(S1) the step of producing an artificial medium containing sannue moth (Antheraea pernyi) pupa extract haemolymph (haemolymph);

(S2) forming artificial eggs on one layer of polypropylene film;

(S3) dispensing the artificial medium prepared in the step (S1) to the artificial egg formed in the step (S2);

(S4) In step (S3), the artificial medium is divided into a single layer of polypropylene film formed with artificial eggs dispensed in half to engage the edges or to overlap the edges and overlap with the other one layer of polypropylene film bag ( manufacturing an artificial egg bag made of a form of a bag);

(S5) applying the abdominal extract of the stink bug on the surface of the artificial egg of the step (S2); And

(S6) inoculated and spawned by inoculation females hatched in artificial eggs of the step (S5).

According to one embodiment, the norinjaeryu al hop jombeol top bridge ant waist norinjae (Riptortus pedestris) as the nature of the host (Ooencyrtus nezarae), Anacanthocoris concoloratus, ground cherry waist norinjae (A. sordidus), genun norinjae (Chauliops fallax ), Rural Cleaver ( Cletus punctiger ), C. rusticus , Alcoccal beetle ( Dolycoris baccarum ), Spotted Round Stinger ( Eysarcoris guttiger ), Homoeocerus unipunctatus , Mungbean Herring Stink ( H. marginiventris ) Leptocorisa chinensis ( Leptocorisa chinensis ), Sugarless Alolin ( Megacopta punctatissimum ), Grass-colored Stink bug ( Nezara antennata ), Southern grass-colored Stink bug ( Nezara viridula ), Horizontal stink bug ( Pezodorus hybneri ), Full- color stink bug ( P. guildinii ) and brown wing stink. Plautia stali ).

According to one embodiment of the present invention, the bag-form artificial egg bag 102 is illustrated as an example in FIG. 6, (i) of a single layer of polypropylene film 100. A hemispherical artificial egg 10 having a diameter of 2.0 to 2.5 mm and a height of 2.0 to 2.5 mm is formed at the top, (ii) the artificial medium 11 is dispensed to the artificial egg, and (iii) the artificial egg is The formed one-ply polypropylene film can be folded in half to engage the edges or overlapped with the other one-ply polypropylene film 101 to engage the edges and seal 12 to produce a bag-form. .

The thickness of the polypropylene film is 0.3 to 0.5mm, preferably 0.4mm, there is no particular limitation to this but can form artificial eggs without tearing the film when forming the artificial eggs in polypropylene, hemispherical shape of the artificial eggs Any thickness can be used as long as it can be maintained and the spawning bee is a thickness capable of spawning.

The artificial egg may be similar to the egg size of the hemipteran, which is a natural host of worms, or be made slightly larger than the egg of the hemipteran. For example, the diameter (b) 2.0 to 2.5mm and the height (a) 2.0 to 2.5mm size, preferably can be manufactured with a diameter of 2.2mm and a height of 2.2mm (see Fig. 7).

According to a preferred embodiment of the present invention, it is possible to make a hemispherical artificial egg having the size using a round rod on the polypropylene film.

The artificial eggs formed on the polypropylene film of the artificial egg bag may be formed at intervals such that the egg beetle can spawn without interfering with each other, for example, 4 to 6 mm, preferably 5 mm on the polypropylene film. It can be formed at intervals between.

The bag-shaped artificial egg bag may be folded in half to form a single polypropylene film in which the artificial egg is formed to engage the edges, or to overlap and seal the edges by overlapping with a single layer of polypropylene film. form) Specifically, the size of the width (c) 4-6 cm, the length (d) 1-3 cm and the height (e) 0.2-1 cm, preferably the width (c) 5 cm, the length (d) 2 cm and the height (e) 0.5 cm It may be size (see FIG. 7), but there is no particular limitation thereto. At this time, 10 to 14, preferably 12 artificial eggs may be formed on the polypropylene of one bag-shaped artificial egg bag.

In the present invention, by sealing the artificial egg bag in the form of a bag-shaped can prevent microbial contamination such as mold from the outside, it is possible to prevent the artificial medium dispensed in the artificial egg is dried by the outside air.

According to a preferred embodiment of the present invention, the artificial medium for artificial breeding worm beetle is a medium containing an anraea pernyi pupa extract as an active ingredient as hemolymph (haemolymph), specifically, a wild silkworm moth ( Antheraea pernyi) ) Pupa haemolymph, egg yolk (chicken yolk), infant formula (Infant formula), Neisenheimer salt solution, penicillin (Penicillin) and Streptomycin (Streptomycin) is characterized by.

According to a preferred embodiment of the present invention, the artificial medium for artificial breeding of swarm beetles is dispensed in an amount of 3 to 5 [mu] l, preferably 4 [mu] l per artificial egg, the amount of which is divided into The spawning beetle eggs are insufficient for spawning if less than 3 μl is used for spawning up to 7 eggs. If the amount is more than 5 μl, the amount of medium is higher than that of artificial eggs. not.

According to a preferred embodiment of the present invention, the egg of the sting beetle can be laid by applying the abdominal extract of the stink bug to the artificial eggs without using the egg of the stink bug which is a natural host of the sting beetle, and the abdominal extract of the stink bug is stink bug female The abdomen of the can be removed and extracted by dipping in an organic solvent for 60 to 80 hours. In this case, examples of the organic solvent include methanol, ethanol, propanol or butanol, and preferably ethanol.

According to one specific embodiment of the present invention, artificial spawning of an egg beetle female may be induced by inoculating an alleged egg beetle female into an artificial egg for artificially breeding an egg beetle.

According to one specific embodiment of the present invention, 15 to 25 female larvae females, preferably 4 to 6 days old, per two artificial egg bags in a bag-shaped artificial roe bag for artificial breeding worms, preferably Crab is characterized by inoculating 20 animals.

According to one specific embodiment of the present invention, female larvae beetles inoculated in artificial roe bags can spawn larvae eggs using artificial eggs and develop into adults through larval and pupa stages. Specifically, the number of eggs spawned in artificial egg bags averaged 185 eggs per two artificial egg bags, and the average recovery rate from adult hatching to adult development was 46.1%, and the growth period was 9-13 from eggs to larvae. It took 16-20 days from egg, pupa to egg, and it seems that pupa takes 7 days on average. In addition, the ratio of adult adults harvested from artificial eggs was 1: 1.64 for males, which was about twice as high as for males.

As a result of artificial breeding using the artificial egg bag for artificial breeding of the larvae beetle according to the present invention as described above, the development was delayed about 3-5 days compared to the natural breeding, and the allegory rate was 46.1%, which is half that of the natural host. Although it appears to be at a level, there is a significant meaning that it is possible to artificially breed its parasitic egg beetle without using egg of stink bug as a natural host. Therefore, artificial breeding of worms using the artificial egg bag is expected to be fully used as a biological control agent in agricultural fields in the future.

On the other hand, the egg spawning beetle artificially scattered in the artificial egg bag may be hatched into the adult through the larva and pupa stage in the artificial egg bag. When the larvae are allegedly adult in the artificial egg sacs, the legumes are sprayed by spraying the artificial insects on the legumes and fruit trees to be applied by opening or cutting the sealed part of the polypropylene film of the artificial egg sacs. The population density of stink bugs can be controlled by using them as biological control agents for stink bugs.

In this way, according to the present invention can be reared in large quantities artificially without using the sting beetle ( Oorencyrtus nezarae ) as a natural host, it can reduce a lot of effort consumed in the natural breeding. In addition, it is expected that the artificial insects can be used as a practical control agent for stink bugs, which are the most damaging pests in legumes and fruit trees, by artificial breeding of worms using these artificial breeding methods.

FIG. 1 is a photograph of breeding ant taurus ( Riptortus pedestris ) in soybean plants.
Figure 2 is a photograph of breeding worms ( Oorencyrtus nezarae ).
Figure 3 is a photograph showing the process of using a natural host egg, Riptortus pedestris eggs for natural spawning of the horned zombie ( Oorencyrtus nezarae ).
Figure 4 is a graph measuring the spawning capacity of the egg worms using natural host eggs.
Figure 5 is a graph showing the cumulative spawning number of the larvae beetle in natural host eggs according to the spawning time.
Figure 6 is a schematic diagram of a process for producing an artificial egg bag for artificial breeding Oorencyrtus nezarae .
Figure 7 is a schematic of the artificial egg bag prepared for artificial breeding Oorencyrtus nezarae .
Figure 8 is a photograph showing the pupa process in the spawning of Oorencyrtus nezarae using artificial eggs.

Hereinafter, examples and the like will be described in detail to help the understanding of the present invention. However, embodiments according to the present invention can be modified in many different forms, the scope of the invention should not be construed as limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Example 1 Experimental Insect Breeding

Saw-legged antler, which is a host of swarm beetle, uses kidney beans as feeding plants in breeding ground (75x75x75cm) in breeding room (26 ± 1 ℃, 35-40% RH, L16: D8) The host eggs were secured by feeding the guinea pigs while being fed with a juice source. FIG. 1 is a photograph of breeding ant taurus ( Riptortus pedestris ) in soybean plants.

On the other hand, the worms were collected from the parasitic eggs by collecting eggs of sawtooth ants and yellow linings from the farmland around Suncheon University in Suncheon-si, Jeollanam-do. The top lid was rounded with a plastic mesh cage (7x7x10cm) treated with a mesh cloth and inoculated with the larvae of the larvae of the host ants, and then inoculated with the adult larvae insects. In D8), paw cultivation continued to maintain the density required for the experiment. Figure 2 is a photograph of breeding worms ( Oorencyrtus nezarae ).

Reference Example 1 Measurement of Spawning Using Natural Host Eggs of Egg Beetles

In order to determine the spawning capacity of eggs in natural hosts, Riptortus pedestris eggs (natural host eggs) were collected and frozen for up to two days (4 ℃), and then allegorized 12 Ten females of Oorencyrtus nezarae were administered within hours to spawn 100 natural host eggs daily (25 ± 1 ° C, 45% RH, L 16: D 8).

Figure 3 is a photograph showing the process of using a natural host egg, Riptortus pedestris for natural spawning of the horned zombie ( Oorencyrtus nezarae ). Here, A is a photograph of the spit ants, yellow sting, natural host eggs (n = 100) for use in natural spawning, B is a photograph of the larvae beetle using the natural host eggs for spawning.

Natural host egg female cock gun Sex ratio
(♀: ♂) n = 10 2.90 ± 1.45 1.60 ± 1.07 4.50 ± 2.07
(0-7) 1.81: 1

As shown in Table 1, as a result of examining the number of eggs spawning eggs per natural host egg of the sawtooth ants, the average adult was 4.50 ± 2.07 eggs per adult eggs, the sex ratio is about The female was more than twice as good at 1: 1.81.

In addition, in order to determine the spawning ability of egg hornet beetle in natural host eggs, the eggs were harvested for up to 30 days, frozen and stored for up to 30 days (4 ℃), and then within 12 hours of allegory. Ten female hog bee bees were administered to spawn 100 natural host eggs daily.

Figure 4 is a graph measuring the spawning capacity of the egg worms using natural host eggs, Figure 5 is a graph showing the cumulative spawning number of egg worms in natural host eggs according to the spawning time. As shown here, spawning was carried out up to 18 days after the administration of parasitic bees, and 65% of the total spawning eggs were spawned within one week, and spawned most on days 5-6. As a result of breeding, a total of 254 adults (85 males and 169 females) were allegorized and their ratio was 1: 1.99. In addition, the cumulative number of eggs per female was found to be 25.4 on average.

Example 1 Breeding of Egg Roast Beetle Using Artificial Eggs

(1) Artificial medium composition

The composition of the artificial medium used in this experiment was described in Lee, H.-P., et al., 1994, Korean J. Entomol. 24 (4): 311-316, Ooencyrtus kuvanae . Based on the contents of the medium composition used in artificial breeding was prepared in the composition shown in Table 2, but was used as a hemolymph ( haemolymph ) pupa ( Antheraea pernyi ) pupa.

As a hemolymph, wild silkworm moth chrysalis was extracted and used for 4 minutes after treatment in water at 60 ° C. The complex nutrients necessary for the development of larvae were used for infant formula.

ingredient weight (%) Antheraea pernyi pupa haemolymph 40 Egg yolk (Chicken yolk) 20 Infant formula 30 Neisenheimer Salt Solution 10 Penicillin 400 units / ml Streptomycin 400 units / ml

(2) artificial egg bag manufacture

Figure 6 is a schematic of the process of manufacturing an artificial egg bag for artificial breeding Oorencyrtus nezarae . Here, (100) is one-ply polypropylene film, (10) is artificial egg, (11) is artificial medium, (101) is another one-ply polypropylene film, and (12) is sealed Form, where 102 is an artificial poultry bag.

Figure 7 is a schematic of the artificial egg bag prepared for artificial breeding Oorencyrtus nezarae . Here, a represents the hemisphere height of the artificial egg, and b represents the diameter of the artificial egg.

Referring to Figures 6 and 7, to make a hemispherical artificial egg (2.2mm × H 2.2mm) (10) by using a round rod iron rod on a 0.4mm thick polypropylene film 100, artificial The spacing between the eggs was 5 mm, so that 12 artificial eggs 10 were formed on one polypropylene film 100. 4 μl of artificial medium (11) was dispensed per artificial egg. In order to supply the oxygen required for the development of the swarm beetle, and to prevent microbial contamination such as mold from the outside, the bag-type is sealed with a vinyl adhesive having a size of 5 cm x 2 cm x 0.5 cm in height. Made in form. All processes for making artificial egg bags were done in a clean bench to prevent contamination of germs.

(3) Scattering Induction Substance Extraction and Application

In order to induce the adult larvae to spawn in artificial eggs, 100 females (about 3.7g) of the anthill antler norin were removed and put in 2,000 µl of ethanol, an organic solvent, and deposited for 72 hours, followed by extraction. An artificial egg formed on a propylene film was applied to each artificial egg surface at a capacity equivalent to one egg (egg equivalent) using a capillary pipet.

(4) Scattering Egg Root Beetles Using Artificial Eggs

20 female eggs of 4th, 5th, and 6th day of each beetle per 2 bags of bag-form artificial eggs formed were exposed for 1 day (24 hours). The scattering rate was measured.

Squirrel female Bag-shape
(12 artificial eggs) Scattered eggs
/ Bag-shape Scattered eggs
/ Artificial eggs Day 4 adults (20) Primary 92 7.67 ± 6.85 Secondary 106 8.33 ± 5.83 Day 5 Adult (20) Primary 108 9.00 ± 5.12 Secondary 46 3.83 ± 4.43 Day 6 adults (20) Primary 117 9.75 ± 6.55 Secondary 86 7.17 ± 6.89 Average 6 92.5 7.71 ± 6.12

As shown in Table 3, the number of eggs scattered from the artificial eggs averaged 92.5 per bag-shaped artificial egg bag, and average 7.71 eggs per artificial egg.

The number of eggs scattered in one artificial egg was 1.7 times more scattered than 4.5 eggs scattered in the eggs of natural host compared to the natural spawning of the reference example 1, which means that the size of the artificial eggs (2.2 mm × H 2.2 mm) It is thought to be more scattered because it is larger than the egg size (about 1.4 mm) of natural hosts.

Figure 8 is a photograph showing the pupa process in the spawning of Oorencyrtus nezarae using artificial eggs. Here, A is a photograph of scattering in artificial eggs of egg worms, B is a photograph showing an egg stalk protruding from the polypropylene film of egg worms and shells of artificial eggs, C is a pupae stage Is a picture showing the stool, and D is a picture showing the pupa stage.

As seen here, the eggs were the eggs of a typical hog bee with a sack prominently protruding from the artificial egg (FIG. 8B). During the life of the larvae it remains attached to the back end of the egg shell, and the produced sludge accumulates here. Upon reaching the stage of degeneration, the urine is discharged into several brown-orange pellets and the insect turns yellowish white (Figure 8C). Chrysalis formed by molting first grayish yellow and later turn black (FIG. 8D).

(5) Development of Egg Roast Beetle Using Artificial Egg Bag

For each day, two female eggs of bag-form artificial bag formed with the above-described artificial eggs were exposed to 20 female eggs of 4th, 5th, and 6th day, respectively, for 1 day (24 hours). After spawning, the number of eggs per egg, the number of larvae developed, solubilization rate, allegory rate, and sex ratio of the adult eggs were examined. The results are shown in Table 4 below.

Squirrel female Eggs spawned from artificial eggs Larva
(%) Number of days from egg to larva Chrysalis (%) Egg to chrysalis (days) Imago
(%) Sex ratio
(♀: ♂) Day 4 Adults (20) 198 128
(64.6%) 9-13 104
(52.5%) 16-20 96
(48.5%) 63: 33 (1.91: 1) Day 5 Adults (20) 154 110
(71.4%) 9-13 79
(51.3%) 16-20 58
(37.7%) 35: 23 (1.52: 1) Day 6 Adults (20) 203 130
(64.0%) 9-13 109
(53.7%) 16-20 102 (50.2%) 61: 41 (1.49: 1) Average 185 122.7 (66.3%) 9-13 97.3 (52.6%) 16-20 85.3 (46.1%) 1.64: 1

As shown in Table 4, the number of eggs scattered in artificial eggs was an average of 185 eggs per two bag-forms, and they hatched and recovered as adults. The average recovery rate was 46.1%, and the growth period was 9-13 days from eggs to larvae. It takes 16-20 days from pupa to pupa, and the average pupa period is 7 days. In addition, the adult ratio of the adult harvested from the artificial eggs was 1: 1.64, which was almost similar to the ratio of 1: 1.81 in the natural host eggs of Reference Example 1.

In this regard, Takasu, K. et al., 1992, Appl. Entomol. Zool. 27 (1): 171-173, described the developmental period of swarm beetles in natural host The results of this study are 2 days of egg period, 4-5 days of larval period, 7-8 days of pupal period, and it takes 13-15 days from egg to allegory, and it is reported that the allegory rate is 96.3%. It took 16-20 days from to fable, which delayed the development of about 3-5 days, and the fable rate was 46.1%, which is about half that of the natural host.

Example 2 Measurement of Scattering Effect According to Hemolymph Type of Artificial Medium

In order to measure the spawning effect according to the types of haemolymph of artificial media, the larvae of the Antheraea pernyi , the chrysalis of the Caligula japonica , the brown-winged larvae, and the larvae of the stinging ants After the extraction and the composition of the medium, the number of spawning and growth patterns for them were compared.

The pupa of chestnut silkworm moth has occurred in Gangwon-do area for two years in 2015 and 2016. During that period, the pupa was collected and stored in cold condition, and then hemolymph was extracted and used. The extracts were taken and used, and brown caterpillars ( Tenebrio molitor ) species were easily purchased because they were raised as edible insects, and the species of insects, which were growing in the laboratory, were used.

After extracting hemolymph from each insect, artificial artificial pouches were prepared in the same manner as in Example 1, using the main ingredient of artificial medium, and then 10 female larvae were administered for 5 days to receive spawning for breeding. It was. The results are shown in Table 5 below.

Haemolymph Scattered Egg Larva
(%) Number of days from egg to larva Chrysalis (%) From egg to pupa
Duration (days) Imago
(%) Chrysalis of Chestnut Silkworm Moth ( Caligula japonica ) 75 57 (76.0%) 21.40 ± 1.20 5 (6.7%) 6.8 ± 0.74 5
(6.7%) Antheraea pernyi pupa 113 65 (57.5%) 10.44 ± 3.37 18 (15.9) 5.5 ± 0.95 18 (15.9%) Brown larva ( Tenebrio molitor ) species 3 0 ※ not hatched Larval Larva ( Riptortus pedestris ) 0 - ※ No scattering is observed

As a result, as shown in Table 5, even in the artificial egg bag using hemolymph extracted from the seedling larvae of the sawtooth ants, the spawning induction reaction was not seen, but in the artificial egg bag using the hemolymph extracted from the brown larvae Eggs were spawned in some eggs, but no further development was made. In the initial experiments, adult eggs were recovered from eggs by using hemolymph extracted from chestnut silkworm moth and chrysalis chrysalis pupa. fell. In case of wild silkworm moth, the larval period of eggs was shortened to some extent, and the solubility was improved.

Example 3 Comparison of Vitality of Egg Beetles Raised in Natural and Artificial Eggs

In order to compare the vitality of the adult larvae beetle reared in natural host eggs and artificial eggs, the length of the hind leg calf for 20 males and females was measured using a dissecting microscope (100X). In addition, it was confirmed that the adult eggs, which were alleged in the artificial eggs, were inoculated again into the eggs and artificial eggs of the natural host to spawn vital eggs. The results are shown in Table 6 below.

Number female Male Artificial eggs 20 278.75 ± 38.21a 262.15 ± 31.28a Natural host egg 20 317.50 ± 42.57a 255.50 ± 34.79a

As shown in Table 6 above, the female length, which was alleged in artificial eggs, was slightly shorter than that in natural eggs ( R. pedestris ), but no significant difference was found. In males, there were almost no differences in artificial eggs or natural eggs. In addition, as a result of inoculating female adult eggs, which were alleged in artificial eggs, into eggs and artificial eggs of natural host, the eggs were spawned with vitality, and new adults could be obtained from them. Therefore, it is thought that the vitality of adult adults developed from artificial eggs does not differ from the vitality of adult adults developed from eggs of natural host.

As a result, this experiment laid the groundwork for artificially raising large numbers of useful algae bee natural beetle beetle of stink bugs. Can be reduced. In addition, it is expected that the artificial eggs can be used as a practical control agent for the sawtooth ant loin norin, which causes the greatest damage in legumes and fruit trees by artificial breeding worms using these artificial eggs.

100: 1 layer of polypropylene film
101: another layer of polypropylene film
102: artificial egg bag
10: artificial egg
11: artificial medium
12: sealing

Claims (9)

A method of artificial breeding Oorencyrtus nezarae characterized in that it comprises the following steps:
(S1) Antheraea pernyi pupa extract containing haemolymph, egg yolk, infant formula, Neilsenheimer salt solution, penicillin and streptoto Preparing an artificial medium consisting of mycin (Streptomycin);
(S2) forming artificial eggs on one layer of polypropylene film;
(S3) dispensing the artificial medium prepared in the step (S1) to the artificial egg formed in the step (S2);
(S4) In step (S3), one layer of polypropylene film having artificial eggs dispensed with artificial medium is folded in half to engage the edges, or the other one layer of polypropylene film is overlapped with the edges to seal and seal the bag ( manufacturing an artificial egg bag made of a form of a bag);
(S5) Riptortus pedestris Peel off the abdomen of females and immersed in an organic solvent for 60 to 80 hours to apply the extracted extract to the artificial egg surface of the step (S2); And
(S6) inoculated and spawned by inoculation females hatched in artificial eggs of the step (S5). delete delete The method of claim 1,
Method of artificial breeding worms beetle, characterized in that the thickness of the polypropylene film in the step (S2) is 0.3 to 0.5mm. The method of claim 1,
In the step (S2) artificial eggs are artificially breeding worms, characterized in that formed on the polypropylene film at intervals of 4 to 6mm. The method of claim 1,
Method of artificial breeding worms beetle, characterized in that in the step (S3) the artificial medium is dispensed in an amount of 3 to 5 μl per artificial egg. The method of claim 1,
In the step (S4), the bag-shape is a method for artificial breeding larva zombie, characterized in that the size of 4 to 6cm in width, 1 to 3cm in height and 0.2 to 1cm in height. delete The method of claim 1,
Method of artificial breeding larvae beetle, characterized in that inoculated 15 to 25 female larvae beetle 4 days to 6 days old hatching per two artificial eggs in the artificial egg in step (S6).

Images